CN104957046A - Tissue rapid propagation cultivation method for dendrobium officinale - Google Patents
Tissue rapid propagation cultivation method for dendrobium officinale Download PDFInfo
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- CN104957046A CN104957046A CN201510469667.0A CN201510469667A CN104957046A CN 104957046 A CN104957046 A CN 104957046A CN 201510469667 A CN201510469667 A CN 201510469667A CN 104957046 A CN104957046 A CN 104957046A
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Abstract
The invention relates to a tissue rapid propagation cultivation method for dendrobium officinale. The tissue rapid propagation cultivation method comprises the following steps: (1) turning on an ultraviolet lamp to sterilize a cultivating table; soaking a sterilized towel in a bromogeramine disinfectant, and wiping the cultivating table by using the towel after soaking for disinfecting the cultivating table; carrying out high temperature sterilization on tweezers frame by adopting an alcohol lamp; preparing materials needed for inoculation, namely, paper, a culture medium and mother seedlings; taking out a mother seedling bottle, disinfecting the mouth of the mother seedling bottle by using the alcohol lamp, taking out the mother seedlings by using tweezers, and placing the mother seedlings on inoculation paper; and separating the mother seedlings on the inoculation paper by using another pair of tweezers, inoculating, and culturing in a seedling culturing room. With the adoption of the tissue rapid propagation cultivation method, the rapid propagation purpose is achieved, the survival rate of seedlings is improved, the yield of dendrobium officinale is increased, and the tissue rapid propagation cultivation method has the characteristics of strong applicability, simple technology and good cultivation effect and is capable of improving the survival rate of the seedlings.
Description
Technical field
The invention belongs to dendrobium candidum Cultivating techniques field, relate to a kind of dendrobium candidum tissue method of quick propagating and cultivating.
Background technology
Dendrobium candidum has another name called: ribbed hedyotis herb, Yunnan iron sheet, be mainly distributed in the ground such as Chinese Anhui, Zhejiang, Fujian.Its stem is used as medicine, and belongs to the yin tonics in tonic: reinforcing stomach reg fluid, nourishing Yin and clearing heat, be first of China nine immortal grass, and the survival rate of existing field dendrobium candidum is low, if extensive cultivation can cause great economic loss.
Summary of the invention
For overcoming above-mentioned technical disadvantages, the invention provides
a kind ofdendrobium candidum tissue method of quick propagating and cultivating, it can realize artificial culture, can ensure the survival rate of dendrobium candidum simultaneously.
The present invention solves the technical method that its technical problem adopts:
a kind ofdendrobium candidum tissue method of quick propagating and cultivating, its incubation step is:
The first step: open uviol lamp and sterilizing is carried out to culture table, closed uviol lamp and open exhausting and sterilizer after 15 minutes, the peculiar smell of generation is taken away;
Second step: use the bromogeramine bacterium thimerosal of the weight ratio 1:7 of bromogeramine bacterium disinfectant and water to wash one's hands, and soak sterilized towel scrubber culture table with the bromogeramine bacterium thimerosal that the weight ratio of bromogeramine bacterium disinfectant and water is 1:2 and carry out disinfection;
3rd step: the take the photograph subrack right with alcolhol burner carries out high-temperature sterilization, the taking the photograph son and after vaccinating lancet reaches assigned temperature, dried in the air and taking the photograph in subrack, can use after cooling of device subject to sterilization;
4th step: get out inoculation material requested and paper, medium, female seedling, prepares to start inoculation;
5th step: take out female seedling bottle, and bottleneck alcolhol burner is carried out disinfection, sterilization two circle, twist-off closure is sterilized two circles again, takes out female seedling be placed on inoculating paper with tweezers;
6th step: use another to take the photograph son and divide seedling to the female Miao Jinhang on inoculating paper, according to length, female seedling is divided into one, two, three seedling, take out new medium, open female seedling bottle bottleneck, change and to take the photograph on new medium that female seedling to receive in female seedling bottle by son that (three seedlings (more than 1.5cm) put 25 strains, secondary seedling (below 1.5cm) puts 22 strains, one time seedling (female seedling ball) puts 18 strains), put into training seedling room after inoculation and cultivate.
The invention has the beneficial effects as follows: the object that can reach Fast-propagation, improve the survival rate of seedling, also accelerate the output of dendrobium candidum simultaneously, have that applicability is strong, concise in technology, tell on, improve the features such as survival rate.
Embodiment
Below in conjunction with embodiment, the present invention is further described.
a kind ofdendrobium candidum tissue method of quick propagating and cultivating, its incubation step is:
The first step: open uviol lamp and sterilizing is carried out to culture table, closed uviol lamp and open exhausting and sterilizer after 15 minutes, the peculiar smell of generation is taken away;
Second step: use the bromogeramine bacterium thimerosal of the weight ratio 1:7 of bromogeramine bacterium disinfectant and water to wash one's hands, and soak sterilized towel scrubber culture table with the bromogeramine bacterium thimerosal that the weight ratio of bromogeramine bacterium disinfectant and water is 1:2 and carry out disinfection;
3rd step: the take the photograph subrack right with alcolhol burner carries out high-temperature sterilization, the taking the photograph son and after vaccinating lancet reaches assigned temperature, dried in the air and taking the photograph in subrack, can use after cooling of device subject to sterilization;
4th step: get out inoculation material requested and paper, medium, female seedling, prepares to start inoculation;
5th step: take out female seedling bottle, and bottleneck alcolhol burner is carried out disinfection, sterilization two circle, twist-off closure is sterilized two circles again, takes out female seedling be placed on inoculating paper with tweezers;
6th step: use another to take the photograph son and divide seedling to the female Miao Jinhang on inoculating paper, according to length, female seedling is divided into one, two, three seedling, take out new medium, open female seedling bottle bottleneck, change and to take the photograph on new medium that female seedling to receive in female seedling bottle by son that (three seedlings (more than 1.5cm) put 25 strains, secondary seedling (below 1.5cm) puts 22 strains, one time seedling (female seedling ball) puts 18 strains), put into training seedling room after inoculation and cultivate.
The present invention can reach the object of Fast-propagation, improves the survival rate of seedling, also accelerates the output of dendrobium candidum simultaneously, has that applicability is strong, concise in technology, tells on, improves the features such as survival rate.
Claims (1)
1.
a kind ofdendrobium candidum tissue method of quick propagating and cultivating, is characterized in that incubation step is:
The first step: open uviol lamp and sterilizing is carried out to culture table, closed uviol lamp and open exhausting and sterilizer after 15 minutes, the peculiar smell of generation is taken away;
Second step: use the bromogeramine bacterium thimerosal of the weight ratio 1:7 of bromogeramine bacterium disinfectant and water to wash one's hands, and soak sterilized towel scrubber culture table with the bromogeramine bacterium thimerosal that the weight ratio of bromogeramine bacterium disinfectant and water is 1:2 and carry out disinfection;
3rd step: the take the photograph subrack right with alcolhol burner carries out high-temperature sterilization, the taking the photograph son and after vaccinating lancet reaches assigned temperature, dried in the air and taking the photograph in subrack, can use after cooling of device subject to sterilization;
4th step: get out inoculation material requested and paper, medium, female seedling, prepares to start inoculation;
5th step: take out female seedling bottle, and bottleneck alcolhol burner is carried out disinfection, sterilization two circle, twist-off closure is sterilized two circles again, takes out female seedling be placed on inoculating paper with tweezers;
6th step: use another to take the photograph son and divide seedling to the female Miao Jinhang on inoculating paper, according to length, female seedling is divided into one, two, three seedling, take out new medium, open female seedling bottle bottleneck, change and to take the photograph on new medium that female seedling to receive in female seedling bottle by son that (three seedlings (more than 1.5cm) put 25 strains, secondary seedling (below 1.5cm) puts 22 strains, one time seedling (female seedling ball) puts 18 strains), put into training seedling room after inoculation and cultivate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510469667.0A CN104957046A (en) | 2015-08-04 | 2015-08-04 | Tissue rapid propagation cultivation method for dendrobium officinale |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510469667.0A CN104957046A (en) | 2015-08-04 | 2015-08-04 | Tissue rapid propagation cultivation method for dendrobium officinale |
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| CN104957046A true CN104957046A (en) | 2015-10-07 |
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| CN201510469667.0A Pending CN104957046A (en) | 2015-08-04 | 2015-08-04 | Tissue rapid propagation cultivation method for dendrobium officinale |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8383881B2 (en) * | 2008-01-08 | 2013-02-26 | National University Of Kaohsiung | Method for producing polyploid plants of orchids |
| CN103385174A (en) * | 2013-07-17 | 2013-11-13 | 宁波神乙草生物科技有限公司 | Tissue culture and inoculation method for dendrobium officinale |
| CN104025929A (en) * | 2014-06-24 | 2014-09-10 | 韶关车八岭农业科技有限公司 | Dendrobium officinale culture system |
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2015
- 2015-08-04 CN CN201510469667.0A patent/CN104957046A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8383881B2 (en) * | 2008-01-08 | 2013-02-26 | National University Of Kaohsiung | Method for producing polyploid plants of orchids |
| CN103385174A (en) * | 2013-07-17 | 2013-11-13 | 宁波神乙草生物科技有限公司 | Tissue culture and inoculation method for dendrobium officinale |
| CN104025929A (en) * | 2014-06-24 | 2014-09-10 | 韶关车八岭农业科技有限公司 | Dendrobium officinale culture system |
Non-Patent Citations (2)
| Title |
|---|
| RAVINDRA B. MALABADI ET.AL.,: "Micropropagation of Dendrobium nobile from shoot tip sections", 《JOURNAL OF PLANT PHYSIOLOGY》 * |
| 张桂海等: "铁皮石斛组培快繁降低污染率的关键措施", 《河北农业科技》 * |
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Application publication date: 20151007 |