CN101773080A - Biological method for inducing polyploid shellfish - Google Patents
Biological method for inducing polyploid shellfish Download PDFInfo
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- CN101773080A CN101773080A CN201010044829A CN201010044829A CN101773080A CN 101773080 A CN101773080 A CN 101773080A CN 201010044829 A CN201010044829 A CN 201010044829A CN 201010044829 A CN201010044829 A CN 201010044829A CN 101773080 A CN101773080 A CN 101773080A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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Abstract
The invention discloses a biological method for inducing polyploid shellfish. Because pro-shell culture in the invention is relatively easy, the survival capability is quite strong, the biological method of the invention is the same with the operation of common group male abalone fertilization and the seedling culture method of common abalone with hybrid triploid female abalone as the female parent, and the triploid rate is higher. Therefore, the biological induction method can more easily obtain an enough number of pro-shell, save largely save medicine, greatly reduce the production cost, has low technical requirement and stable induction effect, and is more suitable to be applied in polyploid scale seedling culture production.
Description
Technical field
The invention discloses a kind of biological method of inducing polyploid shellfish, this method is a kind of abductive approach that uses when inducing polyploid shellfish such as abalone, oyster, scallop in scale, belongs to the multiploid induction technical field.
Background technology
The shellfish triploid that the artificial induction produces has individual big, remarkable economical proterties such as growth is fast, fertility is low, strong stress resistance, meat are good, can improve its commodity value greatly.The abductive approach that uses in the polyploid research at present has three kinds: the physical method that adopts the temperature difference, hydrostatic pressing etc. to handle; Use the chemical method of cytochalasin B (CB), colchicine, 6-dimethylaminopurine drug treating such as (6-DMAP); Induce the tetraploid of generation and common diploid hybrid to obtain triploid biological method.Physical method equipment is simple, cheap, can effectively save derived cost, induces the unsettled difficult problem of effect but fail to solve always, can't use in large-scale production.Chemical method induces effect more stable, especially handle best with cytochalasin B (CB), CB handle to the cell of different times all effectively and pair cell nuclear and organelle influence less, having long action time, inductivity height, advantage that abnormal rate is low, is utilization method the most widely in the present Study on Inducing Polyploid.But all need use expensive medicine owing to grow seedlings at every turn, and there is potential harm in health, the cost of putting in the actual production can be very high.Biological method generally is to produce 100% triploid with tetraploid and diploid hybrid, and is efficient, safe, easy, can large-scale production use, but the tetraploid of cultivating survival at present also has very big difficulty, does not also have the practical application meaning.So above-mentioned POLYPLOID INDUCEMENT method all can't really satisfy the scale polyploid shellfish and grow seedlings and produce requirement inducing aspect effect and the cost.
Summary of the invention
The object of the present invention is to provide a kind of grasp simple to operate, easy, cost biological method low, inducing polyploid shellfish in the scale polyploid is grown seedlings production.
For achieving the above object, solution of the present invention is:
The present invention is a kind of biological method of inducing polyploid shellfish, its concrete operations step is as follows: (1) for the fertilized egg of the male Bao hybridization of the female Bao of first colony and second colony, is that release that the cytochalasin B (CB) of 0.70mg/L-1.00mg/L suppresses its second polar body obtains to hybridize triploid and tests colony with concentration; (2) after hybridization triploid test colony cultivates and forms, identify one by one, through being accredited as triploid female Bao (promptly hybridizing the female Bao of triploid) as maternal and be fertilized direct induced triploid with second colony male Bao Zuowei male parent with a times body analyzer.
The female Bao of described first colony is the female Bao of haliotis discus hannai Ino Dalian colony.
The male Bao of described second colony is the male Bao of dish Bao Ri in-group.
The present invention be a kind of with two different inter-subspecies hybrids in the same species the fertilized egg treated with medicaments and the female triploid of the hybridization colony that will cultivate as female parent, be fertilized as male parent with the male of ordinary group, thus the method for direct induced triploid.
After adopting such scheme, the present invention has the following advantages:
(1) because using, the present invention induce the female Bao of hybridization triploid of acquisition as female parent; obtain the female Bao of tetraploid and compare with inducing as female parent; induce difficulty greatly to reduce, and triploid survival rate is much higher than tetraploid, the close shellfish that can more easily obtain sufficient amount is used for scale breeding production.
(2) since the present invention hybridize triploid induce to handle handle identically with common triploid inducing, and the female Bao of hybridization triploid hybridizes with the male Bao of dish Bao Ri in-group, need not any processing and can directly produce triploid.All need to induce to handle during this and common diploid male and female parent shellfish induced triploid at every turn and compare, can reduce production costs greatly, raise the efficiency.
(3) because the present invention uses the female Bao of hybridization triploid to be fertilized the method for operating of induced triploid the same with the common production method of growing seedlings as maternal with the male Bao Zuowei male parent of dish Bao Ri in-group, general seedling growing process workman just can grasp, so operability aborning is stronger.
(4) because the present invention uses the female Bao of hybridization triploid to come direct induced triploid as maternal with the male Bao Zuowei male parent of dish Bao Ri in-group, can obtain higher relatively triploid rate, and effect stability, be more suitable for utilization in scale breeding is produced.
In sum, the present invention compares with the polyploid shellfish abductive approach of present use, and is simple to operate, can save high amount of drug, greatly reduces production cost, can be directly and induce triploid effectively, help the polyploid shellfish large-scale promotion application.
The present invention is further illustrated below in conjunction with specific embodiment.
Embodiment
The present invention be a kind of with two different inter-subspecies hybrids in the same species the fertilized egg treated with medicaments and the female Bao of hybridization triploid that will cultivate as female parent, be fertilized as male parent with the male of ordinary group, thus the biological method of direct induced triploid.
Its concrete operations step is as follows:
(1) for the fertilized egg of the female Bao of haliotis discus hannai Ino Dalian colony with the male Bao hybridization of dish Bao Ri in-group, with concentration is the female Bao of described hybridization triploid, promptly under water temperature 23-25 ℃, collect and contain 400,000-500,000 fertilized eggs--the fertilized egg of ovum of the female Bao of haliotis discus hannai Ino Dalian colony and the sperm after fertilization of the male Bao of dish Bao Ri in-group is in the 3L plastic containers, the release induced triploid that suppresses the fertilized egg second polar body with cytochalasin B (CB), aqueous solution final volume is 2L, cytochalasin B (CB) concentration is 0.70mg/L-1.00mg/L, induces and handles 10 minutes.After inducing end, wash ovum 2 times, each about 15 minutes with the aqueous solution that contains 0.1% (V/V) methyl-sulfoxide (DMSO).
(2) fertilized egg is cultivated, treat that Bao Miao forms when shell is about 4.0-5.0cm, detect one by one respectively, identify and filter out to such an extent that hybridization triploid Bao continues to cultivate with a times body analyzer; When the sexual gland outward appearance can obviously be differentiated the male and female sex, filter out the female Bao of hybridization triploid once more, continue to cultivate as maternal, and be fertilized, directly induced triploid with the male Bao Zuowei male parent of dish Bao Ri in-group.
Claims (3)
1. the biological method of an inducing polyploid shellfish, it is characterized in that: its concrete operations step is as follows: (1) for the fertilized egg of the male Bao hybridization of the female Bao of first colony and second colony, is that release that the cytochalasin B (CB) of 0.70mg/L-1.00mg/L suppresses its second polar body obtains to hybridize triploid and tests colony with concentration; (2) after hybridization triploid test colony cultivates and forms, identify one by one, through being accredited as triploid female Bao as maternal and be fertilized direct induced triploid with second colony male Bao Zuowei male parent with a times body analyzer.
2. the biological method of inducing polyploid shellfish according to claim 1, it is characterized in that: the female Bao of described first colony is the female Bao of haliotis discus hannai Ino Dalian colony.
3. the biological method of inducing polyploid shellfish according to claim 1 is characterized in that: the male Bao of described second colony is the male Bao of dish Bao Ri in-group.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201010044829A CN101773080A (en) | 2010-01-05 | 2010-01-05 | Biological method for inducing polyploid shellfish |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201010044829A CN101773080A (en) | 2010-01-05 | 2010-01-05 | Biological method for inducing polyploid shellfish |
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| CN101773080A true CN101773080A (en) | 2010-07-14 |
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| CN201010044829A Pending CN101773080A (en) | 2010-01-05 | 2010-01-05 | Biological method for inducing polyploid shellfish |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101627073B1 (en) * | 2015-09-25 | 2016-06-02 | 제주특별자치도(제주특별자치도해양수산연구원장) | Manufacturing method for hybrids of abalone |
| CN106135123A (en) * | 2016-06-30 | 2016-11-23 | 湖南文理学院 | The efficient breeding method of Trionyx sinensis Wiegmann triploid pure lines |
| CN110178799A (en) * | 2019-06-21 | 2019-08-30 | 厦门大学 | A kind of abductive approach of abalone triploid |
-
2010
- 2010-01-05 CN CN201010044829A patent/CN101773080A/en active Pending
Non-Patent Citations (4)
| Title |
|---|
| 严正凛等: "细胞松弛素B诱导盘鲍三倍体的研究", 《台湾海峡》 * |
| 叶玉珍等: "人工复合三倍体鲤的形态学特征及繁殖方式", 《中国水产科学》 * |
| 杨威: "鲍的多倍体育种技术", 《中国海洋报》 * |
| 蔡明夷等: "杂色鲍♀×盘鲍♂杂交受精的细胞学研究", 《厦门大学学报（自然科学版）》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101627073B1 (en) * | 2015-09-25 | 2016-06-02 | 제주특별자치도(제주특별자치도해양수산연구원장) | Manufacturing method for hybrids of abalone |
| CN106135123A (en) * | 2016-06-30 | 2016-11-23 | 湖南文理学院 | The efficient breeding method of Trionyx sinensis Wiegmann triploid pure lines |
| CN110178799A (en) * | 2019-06-21 | 2019-08-30 | 厦门大学 | A kind of abductive approach of abalone triploid |
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Application publication date: 20100714 |