CN103385174A - Tissue culture and inoculation method for dendrobium officinale - Google Patents
Tissue culture and inoculation method for dendrobium officinale Download PDFInfo
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- CN103385174A CN103385174A CN2013103086353A CN201310308635A CN103385174A CN 103385174 A CN103385174 A CN 103385174A CN 2013103086353 A CN2013103086353 A CN 2013103086353A CN 201310308635 A CN201310308635 A CN 201310308635A CN 103385174 A CN103385174 A CN 103385174A
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Abstract
The invention discloses a tissue culture and inoculation method for dendrobium officinale. Firstly, dendrobium officinale plantlets, which are cultured in a culture bottle, are placed on a sheet of inoculation paper. Then, the dendrobium officinale plantlets on the inoculation paper are inoculated to a culture medium to be cultured. The sheet of the inoculation paper is discarded after the inoculation of the dendrobium officinale plantlets on the inoculation paper is finished, and changed into new sheets of the innovation paper to repeat the above steps. The inoculation paper is a paper sheet and is disinfected in advance. Compared with the prior art, the method adopts the paper sheet as a tray in place of an original stainless steel tray, the cost of the paper sheet is lower than the cost of the stainless steel tray and the paper sheet take less space, thereby reducing operation space greatly. The paper sheet after being used can be sold as waste products, thereby omitting an original cleaning procedure, and saving energy consumption and time for the original cleaning procedure. The number of the paper sheet is far larger than the number of the stainless steel tray in the same sterilization device space, thereby reducing the number of the sterilization devices, and reducing the usage cost of the devices, and therefore energy consumption during sterilization is reduced and the production cost is reduced.
Description
Technical field
The invention belongs to dendrobium candidum and cultivate technical field, particularly relate to a kind of candidum tissue culturing inoculation method.
Background technology
at present, the candidum tissue culturing inoculation method of prior art is when inoculation, and the dendrobium candidum seedling that needs first will cultivate is placed in the stainless steel pallet, again the dendrobium candidum seedling in the stainless steel pallet is inoculated in medium and cultivates subsequently, but because a stainless steel pallet uses once, just can reuse after need to again sterilizing, being about to one bottle of dendrobium candidum seedling is placed in a stainless steel pallet, after dendrobium candidum seedling inoculation in the stainless steel pallet is completed, the stainless steel pallet that must more renew when preparing next bottle of inoculation dendrobium candidum seedling, stainless steel pallet after using simultaneously must clean and carry out could continuing to use after high-temperature sterilization, this just causes in seeded process, need a large amount of stainless steel pallets, usually each inoculation workman consumption of a day is nearly hundred, so just need larger space to deposit the stainless steel pallet, operating space is larger, simultaneously also need many sterilizing equipment decontaminating apparatuss supporting, this has just increased the energy consumption in production cost and production process, and then cause production cost higher.
Summary of the invention
The present invention is directed to above problem provides a kind of required operating space less, and the low and energy consumption of production cost is lower candidum tissue culturing inoculation method also.
The present invention overcomes the above problems technical scheme used: a kind of candidum tissue culturing inoculation method, first with one, cultivating the dendrobium candidum seedling that cultivates in bottle is placed on an inoculating paper, again the dendrobium candidum seedling on inoculating paper is inoculated in medium and cultivates subsequently, after dendrobium candidum seedling on inoculating paper has been inoculated, used inoculating paper is thrown away, the inoculating paper that more renews repeats above-mentioned steps; Described inoculating paper is paper, and inoculating paper is in advance through sterilization.
after adopting above method, compared with prior art, the present invention is owing to using paper to be used as pallet, replaced original stainless steel pallet, the cost of paper is lower than the stainless steel pallet, the space that takies is also less, and then significantly reduce operating space, and also can work as waste product after paper uses sells, saved the program of original cleaning, the required energy consumption of original cleaning and time have been saved, and the quantity of paper will be much larger than the quantity of stainless steel pallet in the space of identical sterilizing installation, this just can reduce the quantity of sterilizing installation, reduction equipment use cost, therefore energy consumption relative when sterilizing is also just less, production cost is also just lower.Therefore it is less that the present invention has required operating space, and the low and energy consumption of production cost is lower characteristics also.
As preferably, described inoculating paper is discarded paper; Described inoculating paper is old and useless newspaper, and the cost of discarded paper is lower, and then can further reduce production cost of the present invention.
as preferably, described inoculating paper disinfecting process is placed in cutlery box and builds lid for the inoculating paper that first will cut in advance is stacked, again cutlery box is put into plastic packaging bag, bloat subsequently the air in plastic packaging bag and plastic packaging bag is sealed, then integral body is put in cloth bag again, and with rope, the cloth sack is tightened, last integral body is put into sterilizing getting final product in autoclave, cutlery box is common family expenses cutlery box, after inoculating paper coats like this, can directly by original sterilizing parameter, be undertaken sterilizing in autoclave, need not testing in addition to obtain sterilizing parameter, therefore disinfecting action is easy, need not train in addition the workman.
As preferably, described cloth bag is cotton bag, and rope is cotton cord, can not bond together with plastic packaging bag when cotton bag is sterilizing in autoclave, and reusable; Cotton cord not there will be bonding situation yet when sterilizing simultaneously, is convenient to reuse.
Embodiment
Below in conjunction with specific embodiment, the present invention is described further.
Embodiment 1
A kind of candidum tissue culturing inoculation method, first with one, cultivating the dendrobium candidum seedling that cultivates in bottle is placed on an inoculating paper, again the dendrobium candidum seedling on inoculating paper is inoculated in medium and cultivates subsequently, after dendrobium candidum seedling on inoculating paper has been inoculated, used inoculating paper is thrown away, normally put into prepositioned garbage can, so that the waste recovery purchase, the inoculating paper that more renews repeats above-mentioned steps; Described inoculating paper is paper, and inoculating paper is in advance through sterilization; All the other NM steps are all identical with the inoculation method of prior art.
Described inoculating paper is discarded paper.
Described inoculating paper is old and useless newspaper.
described inoculating paper disinfecting process is placed in cutlery box and builds lid for the inoculating paper that first will cut in advance is stacked, usually cutlery box is common commercially available Domestic plastic cutlery box, the wide * height of long * is 20cm*12cm*6cm, inoculating paper is cut to rectangle usually, requirement can be put into cutlery box and be got final product, again cutlery box is put into plastic packaging bag, bloat subsequently the air in plastic packaging bag and plastic packaging bag is sealed, be that the bottom of both hands along cutlery box from plastic packaging bag pushed subsequently the sealing of plastic packaging bag opening part is got final product to opening part, sealing machine is the sealing machine of commercially available routine, then integral body is put in cloth bag again, and with rope, the cloth sack is tightened, last integral body is put into sterilizing getting final product in autoclave, autoclave is identical with the autoclave of prior art.
Described cloth bag is cotton bag, and rope is cotton cord; Cotton bag and cotton cord are commercially available prod.
Embodiment 2
A kind of candidum tissue culturing inoculation method, first with one, cultivating the dendrobium candidum seedling that cultivates in bottle is placed on an inoculating paper, again the dendrobium candidum seedling on inoculating paper is inoculated in medium and cultivates subsequently, after dendrobium candidum seedling on inoculating paper has been inoculated, used inoculating paper is thrown away, normally put into prepositioned garbage can, so that the waste recovery purchase, the inoculating paper that more renews repeats above-mentioned steps; Described inoculating paper is paper, and inoculating paper is in advance through sterilization; All the other NM steps are all identical with the inoculation method of prior art.
Described inoculating paper is discarded paper (this example is the discarded examination paper of school).
described inoculating paper disinfecting process is placed in cutlery box and builds lid for the inoculating paper that first will cut in advance is stacked, usually cutlery box is common commercially available Domestic plastic cutlery box, the wide * height of long * is 20cm*12cm*6cm, inoculating paper is cut to rectangle usually, requirement can be put into cutlery box and be got final product, again cutlery box is put into plastic packaging bag, bloat subsequently the air in plastic packaging bag and plastic packaging bag is sealed, be that the bottom of both hands along cutlery box from plastic packaging bag pushed subsequently the sealing of plastic packaging bag opening part is got final product to opening part, sealing machine is the sealing machine of commercially available routine, then integral body is put in cloth bag again, and with rope, the cloth sack is tightened, last integral body is put into sterilizing getting final product in autoclave, autoclave is identical with the autoclave of prior art.
Described cloth bag is cotton bag, and rope is cotton cord.
Embodiment 3
A kind of candidum tissue culturing inoculation method, first with one, cultivating the dendrobium candidum seedling that cultivates in bottle is placed on an inoculating paper, again the dendrobium candidum seedling on inoculating paper is inoculated in medium and cultivates subsequently, after dendrobium candidum seedling on inoculating paper has been inoculated, used inoculating paper is thrown away, normally put into prepositioned garbage can, so that the waste recovery purchase, the inoculating paper that more renews repeats above-mentioned steps; Described inoculating paper is paper, and inoculating paper is in advance through sterilization; All the other NM steps are all identical with the inoculation method of prior art.
Described inoculating paper is discarded paper (this example is discarded books or homework book).
described inoculating paper disinfecting process is placed in cutlery box and builds lid for the inoculating paper that first will cut in advance is stacked, usually cutlery box is common commercially available Domestic plastic cutlery box, the wide * height of long * is 20cm*12cm*6cm, inoculating paper is cut to rectangle usually, requirement can be put into cutlery box and be got final product, again cutlery box is put into plastic packaging bag, bloat subsequently the air in plastic packaging bag and plastic packaging bag is sealed, be that the bottom of both hands along cutlery box from plastic packaging bag pushed subsequently the sealing of plastic packaging bag opening part is got final product to opening part, sealing machine is the sealing machine of commercially available routine, then integral body is put in cloth bag again, and with rope, the cloth sack is tightened, last integral body is put into sterilizing getting final product in autoclave, autoclave is identical with the autoclave of prior art.
Described cloth bag is cotton bag, and rope is cotton cord.
Above embodiment is only preferred embodiment of the present invention, and the present invention is not limited only to above embodiment and has also allowed other structural change, allly changes in rights to independence claimed range of the present invention, all belongs to protection domain of the present invention.
Claims (5)
1. candidum tissue culturing inoculation method, it is characterized in that: first with one, cultivate the dendrobium candidum seedling that cultivates in bottle and be placed on an inoculating paper, again the dendrobium candidum seedling on inoculating paper is inoculated in medium and cultivates subsequently, after dendrobium candidum seedling on inoculating paper has been inoculated, used inoculating paper is thrown away, the inoculating paper that more renews repeats above-mentioned steps; Described inoculating paper is paper, and inoculating paper is in advance through sterilization.
2. candidum tissue culturing inoculation method according to claim 1 is characterized in that: described inoculating paper is discarded paper.
3. candidum tissue culturing inoculation method according to claim 2, it is characterized in that: described inoculating paper is old and useless newspaper.
4. candidum tissue culturing inoculation method according to claim 1, it is characterized in that: described inoculating paper disinfecting process is placed in cutlery box and builds lid for the inoculating paper that first will cut in advance is stacked, again cutlery box is put into plastic packaging bag, bloat subsequently the air in plastic packaging bag and plastic packaging bag is sealed, then integral body is put in cloth bag again, and with rope, the cloth sack is tightened, last integral body is put into sterilizing getting final product in autoclave.
5. candidum tissue culturing inoculation method according to claim 4, it is characterized in that: described cloth bag is cotton bag, rope is cotton cord.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310308635.3A CN103385174B (en) | 2013-07-17 | 2013-07-17 | Candidum tissue culturing inoculation method |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201310308635.3A CN103385174B (en) | 2013-07-17 | 2013-07-17 | Candidum tissue culturing inoculation method |
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| CN103385174A true CN103385174A (en) | 2013-11-13 |
| CN103385174B CN103385174B (en) | 2015-11-18 |
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| CN201310308635.3A Expired - Fee Related CN103385174B (en) | 2013-07-17 | 2013-07-17 | Candidum tissue culturing inoculation method |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104957046A (en) * | 2015-08-04 | 2015-10-07 | 韶关车八岭农业科技有限公司 | Tissue rapid propagation cultivation method for dendrobium officinale |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4855236A (en) * | 1983-07-26 | 1989-08-08 | P. B. Ind. Plant Biotech Industries Ltd. | Process for plant tissue culture propagation |
| CN1631109A (en) * | 2004-12-29 | 2005-06-29 | 中国科学院华南植物园 | High quality germchit tissure culturing and rapid breeding method of Dendrobium sp. |
| CN101558740A (en) * | 2009-05-21 | 2009-10-21 | 上海汤振生物技术有限公司 | Method for hormone-free scale culture and production of dendrobium officinale embryo dry product |
| CN102835318A (en) * | 2012-10-08 | 2012-12-26 | 荆树汉 | Technology for rapid sugar-free micropropagation of dendrobium officinale kimura et migo under non-aseptic condition |
-
2013
- 2013-07-17 CN CN201310308635.3A patent/CN103385174B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4855236A (en) * | 1983-07-26 | 1989-08-08 | P. B. Ind. Plant Biotech Industries Ltd. | Process for plant tissue culture propagation |
| CN1631109A (en) * | 2004-12-29 | 2005-06-29 | 中国科学院华南植物园 | High quality germchit tissure culturing and rapid breeding method of Dendrobium sp. |
| CN101558740A (en) * | 2009-05-21 | 2009-10-21 | 上海汤振生物技术有限公司 | Method for hormone-free scale culture and production of dendrobium officinale embryo dry product |
| CN102835318A (en) * | 2012-10-08 | 2012-12-26 | 荆树汉 | Technology for rapid sugar-free micropropagation of dendrobium officinale kimura et migo under non-aseptic condition |
Non-Patent Citations (1)
| Title |
|---|
| 浙江省质量技术监督局: "植物种苗组培快繁技术规程:浙江省地方标准DB33/T 752-2009", 《食品伙伴网》, 10 August 2009 (2009-08-10), pages 13 - 1 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104957046A (en) * | 2015-08-04 | 2015-10-07 | 韶关车八岭农业科技有限公司 | Tissue rapid propagation cultivation method for dendrobium officinale |
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| CN103385174B (en) | 2015-11-18 |
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