CN103385174B - Candidum tissue culturing inoculation method - Google Patents
Candidum tissue culturing inoculation method Download PDFInfo
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- CN103385174B CN103385174B CN201310308635.3A CN201310308635A CN103385174B CN 103385174 B CN103385174 B CN 103385174B CN 201310308635 A CN201310308635 A CN 201310308635A CN 103385174 B CN103385174 B CN 103385174B
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Abstract
A kind of candidum tissue culturing inoculation method, first cultivating one the dendrobium candidum seedling cultivated in bottle is placed on an inoculating paper, again the dendrobium candidum seedling on inoculating paper is inoculated in medium subsequently and cultivates, used inoculating paper is thrown away after having inoculated by the dendrobium candidum seedling on inoculating paper, and the inoculating paper more renewed repeats above-mentioned steps, described inoculating paper is paper, and inoculating paper is in advance through sterilization, compared with prior art, the present invention is used as pallet owing to using paper, instead of original stainless steel pallet, then the cost of paper is lower than stainless steel pallet, the space taken is also less, and then significantly reduce operating space, and paper also can work as waste product sale after using, eliminate the program of original cleaning, save the energy consumption needed for original cleaning and time, and the quantity of paper will much larger than the quantity of stainless steel pallet in the space of identical sterilizing installation, this just can reduce the quantity of sterilizing installation, reduce equipment use cost, therefore energy consumption relative during sterilizing is also just less, production cost is also just lower.
Description
Technical field
The invention belongs to dendrobium candidum Cultivating techniques field, particularly relate to a kind of candidum tissue culturing inoculation method.
Background technology
At present, the candidum tissue culturing inoculation method of prior art, when inoculating, needs first to be placed in stainless steel pallet by the dendrobium candidum seedling cultivated, is inoculated in medium by the dendrobium candidum seedling in stainless steel pallet more subsequently and cultivates, but because a stainless steel pallet uses once, just can reuse after needing again to sterilize, be placed in a stainless steel pallet by one bottle of dendrobium candidum seedling, after the dendrobium candidum seedling in stainless steel pallet has been inoculated, the stainless steel pallet that must more renew when preparing inoculation next bottle of dendrobium candidum seedling, stainless steel pallet after simultaneously using must clean and could continue use after carrying out high-temperature sterilization, this just causes in seeded process, need a large amount of stainless steel pallets, usual each inoculation workman consumption of a day reaches hundred, so just larger space is needed to deposit stainless steel pallet, operating space is larger, also need multiple stage sterilizing equipment decontaminating apparatus supporting simultaneously, this adds increased the energy consumption in production cost and production process, and then cause production cost higher.
Summary of the invention
The present invention is directed to above problem provides a kind of required operating space less, the candidum tissue culturing inoculation method that production cost is lower and energy consumption is also lower.
The present invention overcomes the above problems technical scheme used: a kind of candidum tissue culturing inoculation method, first cultivating one the dendrobium candidum seedling cultivated in bottle is placed on an inoculating paper, again the dendrobium candidum seedling on inoculating paper is inoculated in medium subsequently and cultivates, used inoculating paper is thrown away after having inoculated by the dendrobium candidum seedling on inoculating paper, and the inoculating paper more renewed repeats above-mentioned steps; Described inoculating paper is paper, and inoculating paper is in advance through sterilization.
After adopting above method, compared with prior art, the present invention is used as pallet owing to using paper, instead of original stainless steel pallet, then the cost of paper is lower than stainless steel pallet, the space taken is also less, and then significantly reduce operating space, and paper also can work as waste product sale after using, eliminate the program of original cleaning, save the energy consumption needed for original cleaning and time, and the quantity of paper will much larger than the quantity of stainless steel pallet in the space of identical sterilizing installation, this just can reduce the quantity of sterilizing installation, reduce equipment use cost, therefore energy consumption relative during sterilizing is also just less, production cost is also just lower.Therefore to have required operating space less in the present invention, the feature that production cost is lower and energy consumption is also lower.
As preferably, described inoculating paper is discarded paper; Described inoculating paper is old and useless newspaper, then the cost of discarded paper is lower, and then can reduce production cost of the present invention further.
As preferably, described inoculating paper disinfecting process is first to be placed on stacked for the inoculating paper cut in advance in cutlery box and to build lid, again cutlery box is put into plastic packaging bag, bloat the air in plastic packaging bag subsequently and plastic packaging bag is sealed, then entirety is put in cloth bag again, and with rope, cloth sack is tightened, last entirety puts into sterilizing in autoclave, cutlery box is common family expenses cutlery box, then inoculating paper coated like this after, directly sterilizing can be carried out by original sterilizing parameter in autoclave, without the need to carrying out experiment in addition to obtain sterilizing parameter, therefore disinfecting action is easy, workman need not be trained in addition.
As preferably, described cloth bag is cotton bag, and rope is cotton cord, then cotton bag can not bond together with plastic packaging bag during sterilizing in autoclave, and reusable; Cotton cord also there will not be bonding situation when sterilizing simultaneously, is convenient to reuse.
Embodiment
Below in conjunction with specific embodiment, the present invention is described further.
Embodiment 1
A kind of candidum tissue culturing inoculation method, first cultivating one the dendrobium candidum seedling cultivated in bottle is placed on an inoculating paper, again the dendrobium candidum seedling on inoculating paper is inoculated in medium subsequently and cultivates, used inoculating paper is thrown away after having inoculated by the dendrobium candidum seedling on inoculating paper, normally put into prepositioned garbage can, so that waste recovery is purchased, the inoculating paper more renewed repeats above-mentioned steps; Described inoculating paper is paper, and inoculating paper is in advance through sterilization; All the other NM steps are all identical with the inoculation method of prior art.
Described inoculating paper is discarded paper.
Described inoculating paper is old and useless newspaper.
Described inoculating paper disinfecting process is first to be placed on stacked for the inoculating paper cut in advance in cutlery box and to build lid, usual cutlery box is common commercially available Domestic plastic cutlery box, the wide * height of long * is 20cm*12cm*6cm, inoculating paper is cut to rectangle usually, requirement can put into cutlery box, again cutlery box is put into plastic packaging bag, bloat the air in plastic packaging bag subsequently and plastic packaging bag is sealed, namely both hands are pushed along cutlery box from the bottom of plastic packaging bag to opening part and are sealed by plastic packaging bag opening part subsequently, sealing machine is the sealing machine of commercially available routine, then entirety is put in cloth bag again, and with rope, cloth sack is tightened, last entirety puts into sterilizing in autoclave, autoclave is identical with the autoclave of prior art.
Described cloth bag is cotton bag, and rope is cotton cord; Cotton bag and cotton cord are commercially available prod.
Embodiment 2
A kind of candidum tissue culturing inoculation method, first cultivating one the dendrobium candidum seedling cultivated in bottle is placed on an inoculating paper, again the dendrobium candidum seedling on inoculating paper is inoculated in medium subsequently and cultivates, used inoculating paper is thrown away after having inoculated by the dendrobium candidum seedling on inoculating paper, normally put into prepositioned garbage can, so that waste recovery is purchased, the inoculating paper more renewed repeats above-mentioned steps; Described inoculating paper is paper, and inoculating paper is in advance through sterilization; All the other NM steps are all identical with the inoculation method of prior art.
Described inoculating paper is discarded paper (examination paper that this example is discarded for school).
Described inoculating paper disinfecting process is first to be placed on stacked for the inoculating paper cut in advance in cutlery box and to build lid, usual cutlery box is common commercially available Domestic plastic cutlery box, the wide * height of long * is 20cm*12cm*6cm, inoculating paper is cut to rectangle usually, requirement can put into cutlery box, again cutlery box is put into plastic packaging bag, bloat the air in plastic packaging bag subsequently and plastic packaging bag is sealed, namely both hands are pushed along cutlery box from the bottom of plastic packaging bag to opening part and are sealed by plastic packaging bag opening part subsequently, sealing machine is the sealing machine of commercially available routine, then entirety is put in cloth bag again, and with rope, cloth sack is tightened, last entirety puts into sterilizing in autoclave, autoclave is identical with the autoclave of prior art.
Described cloth bag is cotton bag, and rope is cotton cord.
Embodiment 3
A kind of candidum tissue culturing inoculation method, first cultivating one the dendrobium candidum seedling cultivated in bottle is placed on an inoculating paper, again the dendrobium candidum seedling on inoculating paper is inoculated in medium subsequently and cultivates, used inoculating paper is thrown away after having inoculated by the dendrobium candidum seedling on inoculating paper, normally put into prepositioned garbage can, so that waste recovery is purchased, the inoculating paper more renewed repeats above-mentioned steps; Described inoculating paper is paper, and inoculating paper is in advance through sterilization; All the other NM steps are all identical with the inoculation method of prior art.
Described inoculating paper is discarded paper (this example is discarded books or homework book).
Described inoculating paper disinfecting process is first to be placed on stacked for the inoculating paper cut in advance in cutlery box and to build lid, usual cutlery box is common commercially available Domestic plastic cutlery box, the wide * height of long * is 20cm*12cm*6cm, inoculating paper is cut to rectangle usually, requirement can put into cutlery box, again cutlery box is put into plastic packaging bag, bloat the air in plastic packaging bag subsequently and plastic packaging bag is sealed, namely both hands are pushed along cutlery box from the bottom of plastic packaging bag to opening part and are sealed by plastic packaging bag opening part subsequently, sealing machine is the sealing machine of commercially available routine, then entirety is put in cloth bag again, and with rope, cloth sack is tightened, last entirety puts into sterilizing in autoclave, autoclave is identical with the autoclave of prior art.
Described cloth bag is cotton bag, and rope is cotton cord.
Above embodiment is only preferred embodiment of the present invention, the present invention is not limited only to above embodiment and has also allowed other structure to change, and allly changes in rights to independence claimed range of the present invention, all belongs to scope.
Claims (3)
1. the inoculation method of a candidum tissue culturing, it is characterized in that: first cultivate one the dendrobium candidum seedling cultivated in bottle and be placed on an inoculating paper, again the dendrobium candidum seedling on inoculating paper is inoculated in medium subsequently and cultivates, used inoculating paper is thrown away after having inoculated by the dendrobium candidum seedling on inoculating paper, and the inoculating paper more renewed repeats above-mentioned steps; Described inoculating paper is paper, and inoculating paper is in advance through sterilization; Described inoculating paper is discarded paper; Described inoculating paper disinfecting process is first to be placed on stacked for the inoculating paper cut in advance in cutlery box and to build lid, again cutlery box is put into plastic packaging bag, bloat the air in plastic packaging bag subsequently and plastic packaging bag is sealed, then entirety is put in cloth bag again, and with rope, cloth sack is tightened, last entirety puts into sterilizing in autoclave.
2. the inoculation method of candidum tissue culturing according to claim 1, is characterized in that: described inoculating paper is old and useless newspaper.
3. the inoculation method of candidum tissue culturing according to claim 1, is characterized in that: described cloth bag is cotton bag, and rope is cotton cord.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201310308635.3A CN103385174B (en) | 2013-07-17 | 2013-07-17 | Candidum tissue culturing inoculation method |
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| CN201310308635.3A CN103385174B (en) | 2013-07-17 | 2013-07-17 | Candidum tissue culturing inoculation method |
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| CN103385174A CN103385174A (en) | 2013-11-13 |
| CN103385174B true CN103385174B (en) | 2015-11-18 |
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| CN104957046A (en) * | 2015-08-04 | 2015-10-07 | 韶关车八岭农业科技有限公司 | Tissue rapid propagation cultivation method for dendrobium officinale |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4855236A (en) * | 1983-07-26 | 1989-08-08 | P. B. Ind. Plant Biotech Industries Ltd. | Process for plant tissue culture propagation |
| CN1631109A (en) * | 2004-12-29 | 2005-06-29 | 中国科学院华南植物园 | High quality germchit tissure culturing and rapid breeding method of Dendrobium sp. |
| CN101558740A (en) * | 2009-05-21 | 2009-10-21 | 上海汤振生物技术有限公司 | Method for hormone-free scale culture and production of dendrobium officinale embryo dry product |
| CN102835318A (en) * | 2012-10-08 | 2012-12-26 | 荆树汉 | Technology for rapid sugar-free micropropagation of dendrobium officinale kimura et migo under non-aseptic condition |
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2013
- 2013-07-17 CN CN201310308635.3A patent/CN103385174B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4855236A (en) * | 1983-07-26 | 1989-08-08 | P. B. Ind. Plant Biotech Industries Ltd. | Process for plant tissue culture propagation |
| CN1631109A (en) * | 2004-12-29 | 2005-06-29 | 中国科学院华南植物园 | High quality germchit tissure culturing and rapid breeding method of Dendrobium sp. |
| CN101558740A (en) * | 2009-05-21 | 2009-10-21 | 上海汤振生物技术有限公司 | Method for hormone-free scale culture and production of dendrobium officinale embryo dry product |
| CN102835318A (en) * | 2012-10-08 | 2012-12-26 | 荆树汉 | Technology for rapid sugar-free micropropagation of dendrobium officinale kimura et migo under non-aseptic condition |
Non-Patent Citations (1)
| Title |
|---|
| 植物种苗组培快繁技术规程:浙江省地方标准DB33/T 752-2009;浙江省质量技术监督局;《食品伙伴网》;20090810;第13.1节 * |
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