CN101463318B - Technique for processing substitute of culture dish for plant tissue culture - Google Patents
Technique for processing substitute of culture dish for plant tissue culture Download PDFInfo
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- CN101463318B CN101463318B CN 200910064008 CN200910064008A CN101463318B CN 101463318 B CN101463318 B CN 101463318B CN 200910064008 CN200910064008 CN 200910064008 CN 200910064008 A CN200910064008 A CN 200910064008A CN 101463318 B CN101463318 B CN 101463318B
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- cotton
- plant tissue
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- tissue culture
- culture dish
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Abstract
The invention belongs to the technical field of the plant tissue breeding, in particular to disclosing the processing technique of a substitute of a culture dish for the plant tissue breeding. The course of working is as follows: (1) a cotton fabric or a medical gauze is taken and is tightly sealed; (2) when the pressure is 1.1-1.2kg/cm<3>, the temperature is 121-126 DEG C and the sterilization is 0.5-2 hours; (3) after sterilization, the temperature is kept within 90-150 DEG C, the cotton fabric or the medical gauze can be used directly for inoculation after drying for 0.5-2 hours. Compared with the prior art, the invention is original; in the invention, the cotton or the medical gauge with low price and extensive source is successfully in place of traditional culture dishes, dishes, stainless steel trays and the like after being processed by the special technique and used for inoculation medium, which both saves the time, increases the work efficiency and indirectly reduces the cost.
Description
Technical field
The invention belongs to field of plant tissue culture technique, be specifically related to the complete processing that the surrogate of culture dish is used in a kind of plant tissue culture.
Background technology
Plant tissue culture technique is single because of its research material source, clone heredity, the background unanimity, economical convenient, the efficient height, condition is controlled, error is little, growth is fast, the cycle is short, repeatability is strong, anniversary test or production can in a planned way be turned out the good seed of some amount uniform quality in a short time, thereby caused extensive attention both domestic and external.Plant tissue culture technique is very general now, has become one of main means that famous-brand and high-quality plant factorization grows seedlings.And the culture dish of inoculation usefulness is the sterile carrier of tissue culture on the worktable that sterilizable material separates, cutting is used.At present, main in the tissue culture inoculation these are inoculated vessel otherwise are easy to fragmentation with inoculation culture ware, plate, stainless steel plate, otherwise its area is limited, and the time spent is frequently changed, and has had a strong impact on speed, has improved cost.
Summary of the invention
For overcoming the deficiencies in the prior art part, the object of the present invention is to provide a kind of plant tissue culture complete processing of the surrogate of culture dish.
For achieving the above object, the technical scheme that the present invention takes is as follows: a kind of plant tissue culture complete processing of the surrogate of culture dish, and its course of processing is as follows:
(1) get a cotton or hospital gauze, and with its tight sealing;
(2) carry out autoclaving, treat that pressure is 1.1～1.2kg/cm
3, when temperature is 121～126 ℃, sterilization 0.5～2h;
(3) after the sterilization, temperature remains on 90～150 ℃, can use for inoculation behind oven dry 0.5～2h.
Further, when cotton or hospital gauze suffer unexpected artificial destruction, be unlikely and cause the wasting of resources, the described course of processing (1) is operated according to the following procedure: get a cotton or hospital gauze, cotton or hospital gauze are cut into long 0.8m, wide 0.45m, fold growth 0.8m, wide 0.15m again; Again both sides continuously in the middle of folding to 3 times, be converted into long 0.09m, the rectangle of wide 0.15m; With its tight sealing.
As optimization, pressure is 1.15kg/cm in the described course of processing (2)
3, when temperature was 123 ℃, disinfecting time was 1h.
Further optimize, after the sterilization, when temperature remained on 110 ℃, oven dry 1h can use for inoculation in the described course of processing (3).
As preferably, the cotton described in the described course of processing (1) or hospital gauze are cotton or the hospital gauze of quality tight type.
When tight sealing cotton or hospital gauze, with newspaper that its sealing is tight earlier, mouth is tightened with cotton rope in the back in the described course of processing (1).
A kind of plant tissue culture of handling through complete processing of the present invention is with the using method of the surrogate of culture dish: on Bechtop, the cotton of newspaper bag or hospital gauze uncovered newspaper gently with sterile tweezers raise, press from both sides out cotton or hospital gauze, open one side and reach about 0.2m, alternative culture dish of the prior art and so on is as the female bottle of inoculating surfaces inoculation material; And then start cotton or the hospital gauze of using, at this moment the length direction of the cotton of doubling prestack or hospital gauze about two correct and constitute two present inoculating surfaces that reach about 0.2m, can inoculate female bottle of material again; And then start the cotton of using, and an inoculating surfaces that reaches about 0.2m is respectively arranged about the middle position of doubling, can inoculate female bottle material again; With the another side of crossing of translating into, also have three inoculating surfaces that reach about 0.2m, such bag cotton or hospital gauze can be inoculated several bottles of female bottle materials, and the quantity of the female bottle of concrete inoculation is different and different because of amount of capacity, the built-in material concentration of female bottle.
With respect to prior art, it is one new that the present invention creates, cotton or hospital gauze with low price, wide material sources, through special processing technology of the present invention, successfully substitute traditional culture dish, plate, stainless steel plate etc., used for inoculation medium, both saved the space, improve working efficiency, reduced cost again indirectly.
Embodiment
Below the present invention is described in further detail with specific embodiment, but its protection domain is not limited thereto:
Embodiment 1
A kind of plant tissue culture complete processing of the surrogate of culture dish, its course of processing is as follows:
(1) get quality cotton closely, cotton is cut into long 0.8m, wide 0.45m folds growth 0.8m, wide 0.15m again; Middle to folding to both sides again, be converted into long 0.4m, wide 0.15m; Middle to folding to both sides again, be converted into long 0.2m, wide 0.15m; Middle to folding to both sides again, be converted into long 0.09m, the rectangle of wide 0.15m; With after folding cotton tight with the newspaper sealing, tighten mouth with cotton rope;
(2) put the sterilizing-bucket of high-pressure steam sterilizing pan into, in outer pot, add water, open purging valve, boil to a large amount of hot steams ejections and close again, treat that pressure reaches 1.1kg/cm
3, when temperature is 121 ℃, sterilization 2h;
(3) put drying baker into after the sterilization, temperature remains on 90 ℃, can use for inoculation behind the oven dry 2h.
Embodiment 2
A kind of plant tissue culture complete processing of the surrogate of culture dish, its course of processing is as follows:
(1) get quality cotton closely, cotton is cut into long 0.8m, wide 0.45m folds growth 0.8m, wide 0.15m again; Middle to folding to both sides again, be converted into long 0.4m, wide 0.15m; Middle to folding to both sides again, be converted into long 0.2m, wide 0.15m; Middle to folding to both sides again, be converted into long 0.09m, the rectangle of wide 0.15m; With after folding cotton tight with the newspaper sealing, tighten mouth with cotton rope;
(2) put the sterilizing-bucket of high-pressure steam sterilizing pan into, in outer pot, add water, open purging valve, boil to a large amount of hot steams ejections and close again, treat that pressure reaches 1.2kg/cm
3, when temperature is 126 ℃, sterilization 0.5h;
(3) put drying baker into after the sterilization, temperature remains on 150 ℃, can use for inoculation behind the oven dry 0.5h.
Embodiment 3
A kind of plant tissue culture complete processing of the surrogate of culture dish, its course of processing is as follows:
(1) get quality cotton closely, cotton is cut into long 0.8m, wide 0.45m folds growth 0.8m, wide 0.15m again; Middle to folding to both sides again, be converted into long 0.4m, wide 0.15m; Middle to folding to both sides again, be converted into long 0.2m, wide 0.15m; Middle to folding to both sides again, be converted into long 0.09m, the rectangle of wide 0.15m; With after folding cotton tight with the newspaper sealing, tighten mouth with cotton rope;
(2) put the sterilizing-bucket of high-pressure steam sterilizing pan into, in outer pot, add water, open purging valve, boil to a large amount of hot steams ejections and close again, treat that pressure reaches 1.15kg/cm
3, when temperature is 123 ℃, sterilization 1h;
(3) put drying baker into after the sterilization, temperature remains on 110 ℃, can use for inoculation behind the oven dry 1h.
Embodiment 4
With quality hospital gauze replacement closely cotton, other is with embodiment 3.
The various embodiments described above processing and plant tissue culture with surrogate---the using method of cotton or hospital gauze: on Bechtop of culture dish, the cotton of newspaper bag or hospital gauze uncovered newspaper gently with sterile tweezers raise, press from both sides out cotton or hospital gauze, open one side and reach about 0.2m, the female bottle of inoculation material; And then start cotton or the hospital gauze of using, can inoculate female bottle material again; With the another side of crossing of translating into, can inoculate female bottle material again; Such bag cotton or hospital gauze can be inoculated several bottles of female bottle materials, and the quantity of the female bottle of concrete inoculation is different and different because of the amount of capacity of female bottle, the female bottle of interior dress concentration of material.
More than each embodiment all are preferred implementations of the present invention.Be noted that to those skilled in the art at this; on the spiritual basis that does not break away from complete processing of the present invention; obviously be easy to technical scheme of the present invention is done some little improvement; such as replace cotton of the present invention or hospital gauze with cotton yarn; or on the folding method of cotton or hospital gauze, change slightly; such as the length that only changes cotton or hospital gauze and width; or change folding method; even not folding; these all should be considered as the equivalent technologies feature, all should be within protection scope of the present invention.
Claims (5)
1. a plant tissue culture is characterized in that the course of processing is as follows with the complete processing of the surrogate of culture dish:
(1) get a cotton or hospital gauze, cotton or hospital gauze are cut into long 0.8m, wide 0.45m folds growth 0.8m, wide 0.15m again; Again both sides continuously in the middle of folding to 3 times, be converted into long 0.09m, the rectangle of wide 0.15m; With its tight sealing;
(2) carry out autoclaving, treat that pressure is 1.1～1.2kg/cm
3, when temperature is 121～126 ℃, sterilization 0.5～2h;
(3) after the sterilization, temperature remains on 90～150 ℃, can use for inoculation behind oven dry 0.5～2h.
2. a kind of plant tissue culture as claimed in claim 1 is characterized in that with the complete processing of the surrogate of culture dish pressure is 1.15kg/cm in the described course of processing (2)
3, when temperature was 123 ℃, disinfecting time was 1h.
3. a kind of plant tissue culture as claimed in claim 2 is with the complete processing of the surrogate of culture dish, it is characterized in that in the described course of processing (3) that after the sterilization, when temperature remained on 110 ℃, oven dry 1h can be for the inoculation use.
4. use the complete processing of the surrogate of culture dish as any described a kind of plant tissue culture of claim 1～3, it is characterized in that the cotton described in the described course of processing (1) or hospital gauze are cotton or the hospital gauze of quality tight type.
5. a kind of plant tissue culture as claimed in claim 4 is with the complete processing of the surrogate of culture dish, and when it is characterized in that in the described course of processing (1) tight sealing cotton or hospital gauze, with newspaper that its sealing is tight earlier, mouth is tightened with cotton rope in the back.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200910064008 CN101463318B (en) | 2009-01-05 | 2009-01-05 | Technique for processing substitute of culture dish for plant tissue culture |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200910064008 CN101463318B (en) | 2009-01-05 | 2009-01-05 | Technique for processing substitute of culture dish for plant tissue culture |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101463318A CN101463318A (en) | 2009-06-24 |
| CN101463318B true CN101463318B (en) | 2013-07-10 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200910064008 Expired - Fee Related CN101463318B (en) | 2009-01-05 | 2009-01-05 | Technique for processing substitute of culture dish for plant tissue culture |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1084009A (en) * | 1992-08-05 | 1994-03-23 | 丹·麦克勒 | Be used to cultivate plant, particularly the method for tissue culture plant precursor and container thereof |
| CN2265069Y (en) * | 1996-03-21 | 1997-10-22 | 中国科学院化工冶金研究所 | Multi-layer rotary sieve plate type fog state flow plant organic cultivation reactor |
| CN1331283A (en) * | 2000-06-29 | 2002-01-16 | 核工业北京地质研究院 | MF violet-glass tissue culture bottle |
| WO2003052047A1 (en) * | 2001-12-19 | 2003-06-26 | Natural Implant | Device for active phase cell or tissue sampling and uses thereof |
-
2009
- 2009-01-05 CN CN 200910064008 patent/CN101463318B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1084009A (en) * | 1992-08-05 | 1994-03-23 | 丹·麦克勒 | Be used to cultivate plant, particularly the method for tissue culture plant precursor and container thereof |
| CN2265069Y (en) * | 1996-03-21 | 1997-10-22 | 中国科学院化工冶金研究所 | Multi-layer rotary sieve plate type fog state flow plant organic cultivation reactor |
| CN1331283A (en) * | 2000-06-29 | 2002-01-16 | 核工业北京地质研究院 | MF violet-glass tissue culture bottle |
| WO2003052047A1 (en) * | 2001-12-19 | 2003-06-26 | Natural Implant | Device for active phase cell or tissue sampling and uses thereof |
Non-Patent Citations (1)
| Title |
|---|
| 范胜兰.植物组织培养(五).《辽宁农业科学》.1980,(第03期),56-61. * |
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| CN101463318A (en) | 2009-06-24 |
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Granted publication date: 20130710 Termination date: 20140105 |