CN104950119A - Integrated immune reaction plate - Google Patents
Integrated immune reaction plate Download PDFInfo
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- CN104950119A CN104950119A CN201510307143.1A CN201510307143A CN104950119A CN 104950119 A CN104950119 A CN 104950119A CN 201510307143 A CN201510307143 A CN 201510307143A CN 104950119 A CN104950119 A CN 104950119A
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- China
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- reaction
- sample cup
- reaction disk
- disk body
- reaction plate
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Abstract
The invention discloses an integrated immune reaction plate which comprises a reaction plate body, a sample cup and a reaction plate cover, wherein the sample cup is arranged in the reaction plate body; the reaction plate cover is connected with the reaction plate body; the sample cup is positioned between the reaction plate body and the reaction plate cover. In actual use, samples are added into the reaction plate, the reaction plate is placed into a special detecting instrument, the instrument drives the reaction plate to do centrifugal movement, under the action of centrifugal force, the samples in the sample cup enter reaction cavities distributed on the periphery of the reaction plate, a dry-type reagent sealed in the reaction cavities in advance is mixed with the samples and reacts with the samples, and finally report results of the reaction cavities corresponding to projects can be obtained through the detecting instrument. According to the reaction plate, under the condition of needing no other auxiliary materials, detection on various human immune indexes can be automatically finished, the labor is greatly reduced, and errors due to misoperation are correspondingly reduced; all reactions are conducted in the reaction plate, so that pollution to the environment can be avoided.
Description
Technical field
The present invention relates to a kind of immunity analysis instrument reaction tray, specifically relating to a kind of integrated immune reaction tray completed when not needing other any auxiliary material the every immune index detection of human body.
Background technology
In field of immunoassay detection, domestic and international existing routine techniques, normally reagent separate cold storage is deposited, utilized the equipment such as the pipettor of specialty will refrigerate reagent sucking-off during use by professional, and then the sample that this refrigeration reagent injects cuvette to be detected and cuvette is fully reacted, finally again by its situation of change of optical system detection and its content of mensuration or composition.
But traditional filling reagent and collecting sample need to use pipettor, transfer pipet lamp apparatus, generally need the liquid shifting equipment of two or more to complete testing process during detection, there is preliminary work spended time length, complex operation, be not easy to shortcomings such as carrying, easily pollute and make mistakes.
Therefore, need to propose a kind of novel immunity analysis instrument reaction tray.
Summary of the invention
Goal of the invention: in order to overcome the deficiencies in the prior art, the invention provides a kind of when not needing other any auxiliary material for automatic immunity detect integrated immune reaction tray.
Technical scheme: for achieving the above object, the invention provides a kind of integrated immune reaction tray, comprise reaction disk body, sample cup and reaction disk cover, described sample cup is located in described reaction disk body, described reaction disk cover is connected with described reaction disk body, described sample cup is between described reaction disk body and described reaction disk cover, described reaction disk body comprises immunization program reaction chamber successively from outside to inner side, Sample Buffer chamber and sample cup storage chamber, described sample cup storage chamber bottom is provided with turning axle fixed orifice, heat-sealing film bearing is provided with below described sample cup storage chamber, described sample cup storage chamber both sides are respectively equipped with pilot hole, described sample cup comprises sample cup and the heat-sealing film for sealing described sample cup rim of a cup, dilution and magnetic bead is provided with in described sample cup, folded part is provided with above described heat-sealing film, described sample cup is placed in described sample cup storage chamber and the bottom of described folded part and described heat-sealing film bearing are connected, described reaction disk cover comprises adding mouth, be positioned at the register pin of described adding mouth side and be positioned at the exhausr port of described register pin side, described register pin and described pilot hole are connected and described reaction disk cover are connected with described reaction disk body.
Further, the external side of described reaction tray is evenly provided with several immunization program reaction chambers for one week, described immunization program reaction chamber is all connected with described Sample Buffer chamber by kapillary, and described immunization program reaction chamber inside is equipped with the dry type reagent corresponding with each immunization program.
Further, described reaction disk body also comprises zero point correction position, and described zero point correction position is between any two described immunization program reaction chambers.
Further, described reaction disk body adopts circular disk body and described immunization program reaction chamber to adopt circular cavity structure.
Further, the number of described immunization program reaction chamber is no less than 10.
Further, the number of described immunization program reaction chamber is 12.
Further, described folded part length 2 ~ 4cm larger than the length of described sample cup.
Further, described reaction disk cover and described reaction disk body are by being welded to connect.
Further, described reaction disk cover is connected by ultrasonic welding process with described reaction disk body.
Further, the volume of described sample cup storage chamber is greater than the volume of described sample cup.
Beneficial effect: compared with the prior art, the advantage had is in the present invention:
1, the present invention when not needing other any auxiliary material, can complete and detecting the every immune indexes of human body, greatly reducing labour automatically, also correspondingly decreases the error produced because of misoperation;
2, responding in the present invention is all carry out in reaction tray, can not to environment, and each immunization program reacts the detection that also can not have influence on other.
Accompanying drawing explanation
Fig. 1 is the part drawing of immunity analysis instrument reaction tray of the present invention.
Fig. 2 is the part drawing of reaction disk body of the present invention.
Fig. 3 is the part drawing of sample cup of the present invention.
Fig. 4 is the part drawing of reaction disk cover of the present invention.
Embodiment
Below in conjunction with embodiment, the present invention is further described.
With reference to Fig. 1, a kind of integrated immune reaction tray provided by the invention, comprise reaction disk cover 3, reaction disk body 1 and one is deposited in described reaction disk body 1, dilution and magnetic bead are equipped with in inside sample cup 2, described sample cup 2 is connected i.e. described sample cup 2 and deposits in described reaction disk body 1 with described reaction disk body 1, described reaction disk cover 3 is connected with described reaction disk body 1, in the space that described sample cup 2 forms between described reaction disk body 1 and described reaction disk cover 3;
With reference to Fig. 2, state reaction disk body 1 and adopt circular configuration, described reaction disk body 1 comprises immunization program reaction chamber 11 successively from outside to inner side, Sample Buffer chamber 12 and sample cup storage chamber 16, within outside described reaction disk body 1 one week, be evenly provided with several immunization program reaction chambers 11, the number of described immunization program reaction chamber 11 is no less than 10, in embodiments of the present invention, the number of described immunization program reaction chamber 11 is 12, described immunization program reaction chamber 11 all adopts circular cavity structure in addition, described reaction disk body 1 also comprises zero point correction position 18, described zero point correction position 18 is between any two described immunization program reaction chambers 11, the dry type reagent corresponding with immunization program is equipped with in each immunization program reaction chamber 11, so just there are the 12 kind dry type reagent corresponding with 12 kinds of immunization program, described immunization program reaction chamber 11 is all connected with described Sample Buffer chamber 12 by kapillary 13, described kapillary 13 is vertical and described immunization program reaction chamber 11 and described Sample Buffer chamber 12 respectively, the number of described kapillary 13 is identical with the number of described immunization program reaction chamber 11, be 12, described Sample Buffer chamber 12 is circular groove, diluted sample or other materials can be placed in inside, the bottom of described sample cup storage chamber 16 is provided with turning axle fixed orifice 15, the below of described sample cup storage chamber 16 is provided with heat-sealing film bearing 14, described sample cup storage chamber 16 both sides are respectively equipped with pilot hole 17,
With reference to Fig. 3, described sample cup 2 comprises sample cup 22 and the heat-sealing film 21 for sealing described sample cup 22 rim of a cup, dilution and magnetic bead is provided with in described sample cup 22, the manufacturing process of described sample cup 2 is injected by quantitative dilution in described sample cup 22, add magnetic bead subsequently to stir, described heat-sealing film 21 is finally used to be sealed by the rim of a cup of described sample cup 22, folded part 23 is provided with above described heat-sealing film 21, described sample cup 22 is placed in described sample cup storage chamber 16, again the bottom of described folded part 23 and described heat-sealing film bearing 14 are connected, be fixed on by the bottom of described reverse 23 like this in the heat-sealing film bearing 14 of reaction disk body 1, the volume of described sample cup storage chamber 16 is greater than the volume of described sample cup 2, make described sample cup 2 fully can move in sample cup storage part like this, length 2 ~ the 4cm larger than the length of described sample cup 22 of wherein said folded part 23, folded part refers to and is folded by certain length one end of heat-sealing film, because remaining on, the length of described folded part can ensure that heat-sealing film can not avulsed minimum length just,
With reference to Fig. 4, described reaction disk cover 3 comprises adding mouth 31, be positioned at the register pin 32 of described adding mouth 31 side and be positioned at the exhausr port 33 of described register pin 32 side, described reaction disk cover 3 comprises two adding mouths 31, lay respectively at two register pins 32 of two adding mouth sides and lay respectively at two exhausr ports 33 of two register pin sides, described register pin 32 is connected with described pilot hole 17, by described register pin 32 and being connected of described pilot hole 17, described reaction disk cover 3 is located mutually with described reaction disk body 1, last again by between described reaction disk cover 3 and described reaction disk body 1 by being welded to connect, described reaction disk cover 3 is connected by ultrasonic welding process with described reaction disk body 1, described sample cup 2 is just in time between described reaction disk cover 3 and described reaction disk body 1, two adding mouths 31 on described reaction disk cover 3 are positioned at above the rim of a cup of described sample cup.
The using method of the described integrated immune reaction tray that the present invention proposes:
First sample to be tested is joined in sample cup 2 by adding mouth 31;
Then the described integrated immune reaction tray that the present invention proposes is put into special detecting instrument, start detection instrument, detecting instrument can drive the magnetic bead in sample cup 2 to rotate, and the dilution in sample cup 2 and the sample to be tested that just added fully are mixed;
The turning axle of then special detecting instrument can pass turning axle fixed orifice 15, thus is fixed in special detecting instrument by whole immune response dish, and immune response dish can rotate along with the rotation of turning axle;
Under the effect of the centrifugal force brought at High Rotation Speed, volume due to sample cup storage chamber 16 is greater than the volume of sample cup 2, make sample cup 2 fully can move in sample cup storage part like this, therefore sample cup 2 can away from rotation center, the pulling force that the folded part 23 of heat-sealing film 21 is certain because High Rotation Speed has simultaneously, therefore folded part 23 bottom (namely one end of heat-sealing film folds by certain length) can be torn, because remaining on, reaction tray can not ensured that heat-sealing film can not avulsed minimum length and can not being torn by the length of folded part under service condition just, the diluted sample that mixes (namely dilution and the sample to be tested that just added fully mix after material) enter in Sample Buffer chamber 12 under the influence of centrifugal force, because immunization program reaction chamber 11 is all connected with Sample Buffer chamber 12 by kapillary 13, so last diluted sample all enters into immunization program reaction chamber 11 by kapillary 13, because the diluted sample volume in Sample Buffer chamber 12 is greater than the summation of all immunization program reaction chamber 11 volumes, therefore can ensure that all immunization program reaction chambers 11 kinds can both be full of diluted sample, the number of immunization program reaction chamber 11 is 12 in embodiments of the present invention, the dry type reagent corresponding with immunization program is equipped with in 12 immunization program reaction chambers, so just there are the 12 kind dry type reagent corresponding with 12 kinds of immunization program,
Find the zero point correction position 18 of immune response dish kind finally by the sensor in special detecting instrument, successively reaction liquid in each immunization program reaction chamber 11 is detected subsequently, obtain the concrete report of each immunization program.
The above is only the preferred embodiment of the present invention; be noted that for those skilled in the art; under the premise without departing from the principles of the invention, can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.
Claims (10)
1. an integrated immune reaction tray, it is characterized in that: comprise reaction disk body (1), sample cup (2) and reaction disk cover (3), described sample cup (2) is located in described reaction disk body (1), described reaction disk cover (3) is connected with described reaction disk body (1), described sample cup (2) is positioned between described reaction disk body (1) and described reaction disk cover (3), described reaction disk body (1) comprises immunization program reaction chamber (11) successively from outside to inner side, Sample Buffer chamber (12) and sample cup storage chamber (16), described sample cup storage chamber (16) bottom is provided with turning axle fixed orifice (15), described sample cup storage chamber (16) below is provided with heat-sealing film bearing (14), described sample cup storage chamber (16) both sides are respectively equipped with pilot hole (17), described sample cup (2) comprises sample cup (22) and the heat-sealing film (21) for sealing described sample cup (22) rim of a cup, dilution and magnetic bead is provided with in described sample cup (22), described heat-sealing film (21) top is provided with folded part (23), described sample cup (22) is placed in described sample cup storage chamber (16) and the bottom of described folded part (23) and described heat-sealing film bearing (14) are connected, described reaction disk cover (3) comprises adding mouth (31), be positioned at the register pin (32) of described adding mouth (31) side and be positioned at the exhausr port (33) of described register pin (32) side, described register pin (32) and described pilot hole (17) are connected and described reaction disk cover (3) are connected with described reaction disk body (1).
2. integrated immune reaction tray according to claim 1, it is characterized in that: described reaction disk body (1) outside is evenly provided with several immunization program reaction chambers (11) for one week, described immunization program reaction chamber (11) is all connected with described Sample Buffer chamber (12) by kapillary (13), and described immunization program reaction chamber (11) inside is equipped with the dry type reagent corresponding with each immunization program.
3. integrated immune reaction tray according to claim 2, it is characterized in that: described reaction disk body (1) also comprises zero point correction position (18), described zero point correction position (18) is positioned between any two described immunization program reaction chambers (11).
4. integrated immune reaction tray according to claim 1 and 2, is characterized in that: described reaction disk body (1) adopts circular disk body and described immunization program reaction chamber (11) to adopt circular cavity structure.
5. integrated immune reaction tray according to claim 2, is characterized in that: the number of described immunization program reaction chamber (11) is no less than 10.
6. integrated immune reaction tray according to claim 5, is characterized in that: the number of described immunization program reaction chamber (11) is 12.
7. integrated immune reaction tray according to claim 1, is characterized in that: described folded part (23) length 2 ~ 4cm larger than the length of described sample cup (22).
8. integrated immune reaction tray according to claim 1, is characterized in that: described reaction disk cover (3) and described reaction disk body (1) are by being welded to connect.
9. integrated immune reaction tray according to claim 8, is characterized in that: described reaction disk cover (3) is connected by ultrasonic welding process with described reaction disk body (1).
10. integrated immune reaction tray according to claim 1, is characterized in that: the volume of described sample cup storage chamber (16) is greater than the volume of described sample cup (2).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510307143.1A CN104950119A (en) | 2015-06-04 | 2015-06-04 | Integrated immune reaction plate |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510307143.1A CN104950119A (en) | 2015-06-04 | 2015-06-04 | Integrated immune reaction plate |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104950119A true CN104950119A (en) | 2015-09-30 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510307143.1A Pending CN104950119A (en) | 2015-06-04 | 2015-06-04 | Integrated immune reaction plate |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114236129A (en) * | 2021-12-20 | 2022-03-25 | 江苏集萃纳米应用技术研究所有限公司 | In-vitro immunodiagnosis reagent background luminescence processing system |
-
2015
- 2015-06-04 CN CN201510307143.1A patent/CN104950119A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114236129A (en) * | 2021-12-20 | 2022-03-25 | 江苏集萃纳米应用技术研究所有限公司 | In-vitro immunodiagnosis reagent background luminescence processing system |
| CN114236129B (en) * | 2021-12-20 | 2022-09-23 | 江苏集萃中科纳米科技有限公司 | In-vitro immunodiagnosis reagent background luminescence processing system |
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Application publication date: 20150930 |
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| WD01 | Invention patent application deemed withdrawn after publication |