CN104944536A - Method for inhibiting growth of chlorella - Google Patents

Method for inhibiting growth of chlorella Download PDF

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Publication number
CN104944536A
CN104944536A CN201510298352.4A CN201510298352A CN104944536A CN 104944536 A CN104944536 A CN 104944536A CN 201510298352 A CN201510298352 A CN 201510298352A CN 104944536 A CN104944536 A CN 104944536A
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China
Prior art keywords
chlorella
pyrazoles
phenyl
growth
algae
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Pending
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CN201510298352.4A
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Chinese (zh)
Inventor
周泉城
刘娜
冯传兴
张光耀
杨晓玉
常璇
李凡
步雷雷
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Shandong University of Technology
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Shandong University of Technology
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Priority to CN201510298352.4A priority Critical patent/CN104944536A/en
Publication of CN104944536A publication Critical patent/CN104944536A/en
Pending legal-status Critical Current

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Abstract

The invention discloses the application of (2, 4, 6-trihydroxyphenyl)-4-(4-hydroxyphenyl) pyrazoles in inhibiting the algae growth. The (2, 4, 6-trihydroxyphenyl)-4-(4-hydroxyphenyl) pyrazoles can promote the activity of the pectase. Other organisms in the environment are not influenced at all. The method is characterized in that after a certain stage of growth, the chlorella is put into the water. During the initial growth process, the growth of chlorella is inhibited, so that the density of chlorella is kept at a relatively low value. Therefore, the outburst of water blooms is effectively prevented. The method has the advantages of safety, high efficiency, degradation and the like and can be widely applied to natural or landscape waters.

Description

A kind of method suppressing chlorella growth
Technical field
The invention belongs to biology, environmental area, particularly a kind of method suppressing chlorella growth.The invention belongs to ecological algae control technical field, relate to (2,4,6-trihydroxy-phenyl)-4-(4-hydroxy phenyl) application of pyrazoles in suppression algal grown, utilize it to carry out control algae, by promoting the activity of the polygalacturonase that may contain in algae, suppressing algae chlorophyll to grow and causing the damage of DNA, effectively can control the speed of growth and the quality of chlorella, suppress the outburst of wawter bloom.
Background technology
Chlorella is a kind of eucaryon unicellular algae, also be that the first is by micro-algae of artificial culture, there is extremely abundant balanced nutritive ingredient and excellent medical care effect, and be widely used in the aspect such as production of sewage disposal and protective foods, aquaculture bait, livestock feed additive, be described as medicine algae.
In recent years, along with the rapid emergence of algae bio technology, because structure is simple, it is rapid, nutritious to grow, edible again, thus chlorella is produced medicine by as bio-reactor.But its speed of growth is asynchronous with the requirement of bio-reactor rapidly; reaction system is caused to play best effect; and; too fast growth causes wawter bloom; water ecosystem is destroyed; the Algae toxins that algae produces seriously jeopardizes water ecosystem and human-body safety, how effectively to control the algal grown in eutrophication water, prevents wawter bloom from becoming Ecology and protects one of major issue can not avoided with pollution waters restoration.
Existing algae control technology can be divided into physics, chemistry and biological three classes.Wherein physical removal mainly comprises the method such as machinery or artificial salvagings, clay flocculation, be applicable to algal tufa should acute removing, but workload is large, cost is high, also faces the further disposal to collection frond, is unsuitable for the elimination of big area wawter bloom; Chemistry kills algae and mainly applies a certain amount of chemical agent to wawter bloom waters, these class methods are easy to operate, instant effect, but effect duration is short, while killing algae, also other biological produces and poisons in right water body, residual medicament and degradation production thereof inevitably cause secondary pollution, can not big area continue to use; Biological control technology is mainly by being positioned at biology the ingesting or utilizing the flocculation of microorganism to algal tufa or the cracking to frustule to reach the object eliminating algal tufa algal tufa of food chain upstream, but for poisonous algal tufa, be faced with toxin in the biomagnification of food chain or a large amount of releases of toxin in water body, while control algae, also can bring new problems of ecological security.Just make frond grow when how to make algal tufa also not occur and keep suppressing, make algal populations remain on lower density state, be only the key point controlling algal bloom.
In order to solve the problems of the technologies described above, the technical solution that the present invention proposes is: when wawter bloom frond biomass reaches threshold level, start to add (2,4,6-trihydroxy-phenyl)-4-(4-hydroxy phenyl) pyrazoles, just make when rapid growth of chlorella or wawter bloom also do not occur frond grow and keep suppressing, make Measures of Algae in Water Body population remain on lower density state, can not produce other biological in water body simultaneously and poison.
Summary of the invention
The object of the present invention is to provide one (2,4,6-trihydroxy-phenyl)-4-(4-hydroxy phenyl) application of pyrazoles in suppression chlorella growth and wawter bloom.
To achieve the above object, the present invention adopts following technical measures in application process:
1., when chlorella algae liquid absorbancy reaches 0.25, start to add (2,4,6-trihydroxy-phenyl)-4-(4-hydroxy phenyl) pyrazoles;
2.(2,4,6-trihydroxy-phenyl)-4-(4-hydroxy phenyl) to add dosage be 32-64 mg/L to pyrazoles;
3. the input cycle is 9 days.
According to above-mentioned addition manner, chlorella is maintained at low density state, effectively controls Fast-propagation and the outburst preventing wawter bloom.
The mechanism of action of algae-inhibiting agent of the present invention is: (2,4,6-trihydroxy-phenyl)-4-(4-hydroxy phenyl) pyrazoles has and promotes the character of pectinase activity, can improve the growth inhibitory effect of polygalacturonase to chlorella.And polygalacturonase in chlorella, may be contained, and polygalacturonase has significant damaging effect to frustule structure, and the chlorophyll in energy fast degradation frustule, and cause frustule Lipid peroxidation metabolism thus accumulate excessive MDA, thus add (2,4,6-trihydroxy-phenyl)-4-(4-hydroxy phenyl) pyrazoles can play the effect suppressing bead algal grown.
Compared with prior art, the present invention has the following advantages:
1.(2,4,6-trihydroxy-phenyl)-4-(4-hydroxy phenyl) pyrazoles can be degraded in water body, compared with the algicides such as copper sulfate, can not cause secondary pollution;
2.(2,4,6-trihydroxy-phenyl)-4-(4-hydroxy phenyl) pyrazoles compared with other allelochemicals with algistatic activity found, have the advantages that algistatic activity is stronger.
Accompanying drawing explanation
Fig. 1 is (2,4,6-trihydroxy-phenyl)-4-(4-hydroxy phenyl) chlorella growth situation schematic diagram under pyrazoles process.
Embodiment
(2,4,6-trihydroxy-phenyl) used in the present invention-4-(4-hydroxy phenyl) pyrazoles by commercial synthesis (purity is analytical pure), add directly to water body.For a better understanding of the present invention, illustrate content of the present invention further below in conjunction with embodiment, but content of the present invention is not only confined to the following examples.
Embodiment 1:
High-density culture algal inhibition is tested:
Experiment is carried out in 250mL Erlenmeyer flask, being inoculated in 100mL nutrient solution, making its initial algae liquid absorbancy reach 0.25 by being in vigorous long-term chlorella.If experimental group and control group (without algae-inhibiting agent), (2 are added to experimental group, 4,6-trihydroxy-phenyl)-4-(4-hydroxy phenyl) pyrazoles, wherein (2,4,6-trihydroxy-phenyl)-4-(4-hydroxy phenyl) to add dosage be 32mg/L to pyrazoles, adding frequency is once add up the growing state of algae subsequently every day.Add (2,4,6-trihydroxy-phenyl)-4-(4-hydroxy phenyl) after pyrazoles, chlorella growth is suppressed after 9 days in cultivation, and growth inhibition ratio reaches 76.3%(Fig. 1).
Embodiment 2:
High-density culture algal inhibition is tested:
Experiment is carried out in 250mL Erlenmeyer flask, being inoculated in 100mL nutrient solution, making its initial algae liquid absorbancy reach 0.25 by being in vigorous long-term chlorella.If experimental group and control group (without algae-inhibiting agent), three parallel, (2 are added to experimental group, 4,6-trihydroxy-phenyl)-4-(4-hydroxy phenyl) pyrazoles, wherein (2,4,6-trihydroxy-phenyl)-4-(4-hydroxy phenyl) to add dosage be 64 mg/L to pyrazoles, add frequency for once, add up the growing state of algae subsequently every day.Add (2,4,6-trihydroxy-phenyl)-4-(4-hydroxy phenyl) after pyrazoles, chlorella growth is suppressed after 9 days in cultivation, and growth inhibition ratio reaches 74.9%(Fig. 1).

Claims (1)

1. one kind (2,4,6-trihydroxy-phenyl)-4-(4-hydroxy phenyl) application of pyrazoles in suppression algal grown, it is characterized in that:
(1) when chlorella algae liquid absorbancy reaches 0.25, start to add (2,4,6-trihydroxy-phenyl)-4-(4-hydroxy phenyl) pyrazoles;
(2) adding dosage is 32-64mg/L;
(3) the input cycle is 9 days.
CN201510298352.4A 2015-06-03 2015-06-03 Method for inhibiting growth of chlorella Pending CN104944536A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105062946A (en) * 2015-07-27 2015-11-18 山东理工大学 Method for promoting chlorella growth

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1980000082A1 (en) * 1978-06-21 1980-01-24 Otsuka Pharma Co Ltd A-d-galacturonide derivatives and late blight-controlling agent
CN101289246A (en) * 2008-05-20 2008-10-22 云南大学 Applications of pectic enzyme for restraining algal tufa and method
CN101795563A (en) * 2005-12-23 2010-08-04 巴斯夫欧洲公司 The method of control pasture and water
CN103431476A (en) * 2013-09-06 2013-12-11 山东理工大学 Preparation method of clear fresh nectarine juice beverage

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1980000082A1 (en) * 1978-06-21 1980-01-24 Otsuka Pharma Co Ltd A-d-galacturonide derivatives and late blight-controlling agent
CN101795563A (en) * 2005-12-23 2010-08-04 巴斯夫欧洲公司 The method of control pasture and water
CN101289246A (en) * 2008-05-20 2008-10-22 云南大学 Applications of pectic enzyme for restraining algal tufa and method
CN103431476A (en) * 2013-09-06 2013-12-11 山东理工大学 Preparation method of clear fresh nectarine juice beverage

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105062946A (en) * 2015-07-27 2015-11-18 山东理工大学 Method for promoting chlorella growth

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