CN104944535A

CN104944535A - Method for inhibiting growth of chlorella

Info

Publication number: CN104944535A
Application number: CN201510298256.XA
Authority: CN
Inventors: 周泉城; 冯传兴; 刘娜; 张光耀; 常璇; 杨晓玉; 李凡; 步雷雷
Original assignee: Shandong University of Technology
Current assignee: Shandong University of Technology
Priority date: 2015-06-03
Filing date: 2015-06-03
Publication date: 2015-09-30

Abstract

The invention discloses the application of pectase and (2, 4, 6-trihydroxyphenyl)-4-(4-hydroxyphenyl) pyrazoles in inhibiting the growth of chlorella. The pectase is a common enzyme in the food field and can be applied to fruit and vegetable juice clarifying agents. The (2, 4, 6-trihydroxyphenyl)-4-(4-hydroxyphenyl) pyrazoles can promote the activity of the pectase. Other organisms in the environment are not influenced at all. The method is characterized in that after a certain stage of growth, the chlorella is put into the water. During the initial growth process, the growth of chlorella is inhibited, so that the density of chlorella is kept at a relatively low value. Therefore, the outburst of water blooms is effectively prevented. The method has the advantages of safety, high efficiency, degradation and the like and can be widely applied to natural or landscape waters.

Description

Suppress a kind of method of chlorella growth

Technical field

The invention belongs to biology, environmental area, particularly a kind of method suppressing chlorella growth.The invention belongs to ecological algae control technical field, relate to a kind of polygalacturonase and one and there is the compound (2 promoting pectinase activity, 4,6-trihydroxy-phenyl)-4-(4-hydroxy phenyl) application of pyrazoles in suppression algal grown, their synergy is utilized to carry out control algae, cause damage by the suppression that grows algae chlorophyll and DNA, effectively can control the speed of growth and the quality of chlorella, suppress the outburst of wawter bloom.

Background technology

Chlorella is a kind of eucaryon unicellular algae, also be that the first is by micro-algae of artificial culture, there is extremely abundant balanced nutritive ingredient and excellent medical care effect, and be widely used in the aspect such as production of sewage disposal and protective foods, aquaculture bait, livestock feed additive, be described as medicine algae.

In recent years, along with the rapid emergence of algae bio technology, because structure is simple, it is rapid, nutritious to grow, edible again, thus chlorella is produced medicine by as bio-reactor.But its speed of growth is asynchronous with the requirement of bio-reactor rapidly; reaction system is caused to play best effect; and; too fast growth causes wawter bloom; water ecosystem is destroyed; the Algae toxins that algae produces seriously jeopardizes water ecosystem and human-body safety, how effectively to control the algal grown in eutrophication water, prevents wawter bloom from becoming Ecology and protects one of major issue can not avoided with pollution waters restoration.

Existing algae control technology can be divided into physics, chemistry and biological three classes.Wherein physical removal mainly comprises the method such as machinery or artificial salvagings, clay flocculation, be applicable to algal tufa should acute removing, but workload is large, cost is high, also faces the further disposal to collection frond, is unsuitable for the elimination of big area wawter bloom; Chemistry kills algae and mainly applies a certain amount of chemical agent to wawter bloom waters, these class methods are easy to operate, instant effect, but effect duration is short, while killing algae, also other biological produces and poisons in right water body, residual medicament and degradation production thereof inevitably cause secondary pollution, can not big area continue to use; Biological control technology is mainly by being positioned at biology the ingesting or utilizing the flocculation of microorganism to algal tufa or the cracking to frustule to reach the object eliminating algal tufa algal tufa of food chain upstream, but for poisonous algal tufa, be faced with toxin in the biomagnification of food chain or a large amount of releases of toxin in water body, while control algae, also can bring new problems of ecological security.Just make frond grow when how to make algal tufa also not occur and keep suppressing, make algal populations remain on lower density state, be only the key point controlling algal bloom.

To solve the problems of the technologies described above, the technical solution that the present invention proposes is: when wawter bloom frond biomass reaches threshold level, start the material-(2 adding crude substance polygalacturonase He there is pectinase activity promoter action, 4,6-trihydroxy-phenyl)-4-(4-hydroxy phenyl) pyrazoles, just make when rapid growth of chlorella or wawter bloom also do not occur frond grow and keep suppressing, make Measures of Algae in Water Body population remain on lower density state, can not produce other biological in water body simultaneously and poison.

Summary of the invention

The object of the present invention is to provide a kind of polygalacturonase and material-(2,4,6-trihydroxy-the phenyl)-4-(4-hydroxy phenyl with pectinase activity promoter action) application of pyrazoles in suppression chlorella growth and wawter bloom.

To achieve the above object, the present invention adopts following technical measures in application process:

1., when chlorella algae liquid absorbancy reaches 0.25, start to add polygalacturonase and (2,4,6-trihydroxy-phenyl)-4-(4-hydroxy phenyl) pyrazoles;

2. polygalacturonase adds dosage is 640 mg/L;

3. (2,4,6-trihydroxy-phenyl)-4-(4-hydroxy phenyl) to add dosage be 32-96 mg/L to pyrazoles;

4. the input cycle is 9 days.

According to above-mentioned addition manner, chlorella is maintained at low density state, effectively controls Fast-propagation and the outburst preventing wawter bloom.

The mechanism of action of algae-inhibiting agent of the present invention is: polygalacturonase has frustule structure and has significant damaging effect, and the chlorophyll in energy fast degradation frustule, and cause frustule Lipid peroxidation metabolism thus accumulate excessive MDA, thus play the effect suppressing bead algal grown.And (2,4,6-trihydroxy-phenyl)-4-(4-hydroxy phenyl) pyrazoles can promote the activity of polygalacturonase therefore to improve the growth inhibitory effect of polygalacturonase to chlorella.

Compared with prior art, the present invention has the following advantages:

1, polygalacturonase belongs to the product of natural biology, and ecological security is good, can not cause murder by poisoning to other biological in water body;

2, (2,4,6-trihydroxy-phenyl)-4-(4-hydroxy phenyl) pyrazoles can be degraded in water body, compared with the algicides such as copper sulfate, can not cause secondary pollution;

3, polygalacturonase and (2,4,6-trihydroxy-phenyl)-4-(4-hydroxy phenyl) pyrazoles compared with other allelochemicals with algistatic activity found, have the advantages that algistatic activity is stronger.

Accompanying drawing explanation

Fig. 1 is a kind of polygalacturonase and (2,4,6-trihydroxy-phenyl)-4-(4-hydroxy phenyl) chlorella growth situation schematic diagram under pyrazoles process

Embodiment

(2,4,6-trihydroxy-phenyl) used in the present invention-4-(4-hydroxy phenyl) pyrazoles by commercial synthesis (purity is analytical pure), add directly to water body together with polygalacturonase.For a better understanding of the present invention, illustrate content of the present invention further below in conjunction with embodiment, but content of the present invention is not only confined to the following examples.

Embodiment 1:

High-density culture algal inhibition is tested:

Experiment is carried out in 250mL Erlenmeyer flask, being inoculated in 100mL nutrient solution, making its initial algae liquid absorbancy reach 0.25 by being in vigorous long-term chlorella.If experimental group and control group (without algae-inhibiting agent), polygalacturonase and (2 is added to experimental group, 4,6-trihydroxy-phenyl)-4-(4-hydroxy phenyl) pyrazoles, wherein polygalacturonase adds dosage is 640 mg/L, (2,4,6-trihydroxy-phenyl)-4-(4-hydroxy phenyl) to add dosage be 32mg/L to pyrazoles, add frequency for once, add up the growing state of algae subsequently every day.Add polygalacturonase and (2,4,6-trihydroxy-phenyl)-4-(4-hydroxy phenyl) after pyrazoles, chlorella growth is suppressed after 9 days in cultivation, and growth inhibition ratio reaches 25.4%(Fig. 1).

Embodiment 2:

High-density culture algal inhibition is tested:

Experiment is carried out in 250mL Erlenmeyer flask, being inoculated in 100mL nutrient solution, making its initial algae liquid absorbancy reach 0.25 by being in vigorous long-term chlorella.If experimental group and control group (without algae-inhibiting agent), three parallel, polygalacturonase and (2,4,6-trihydroxy-phenyl)-4-(4-hydroxy phenyl is added to experimental group) pyrazoles, wherein polygalacturonase adds dosage is 640 mg/L, (2,4,6-trihydroxy-phenyl)-4-(4-hydroxy phenyl) to add dosage be 96 mg/L to pyrazoles, adding frequency is once add up the growing state of algae subsequently every day.Add polygalacturonase and (2,4,6-trihydroxy-phenyl)-4-(4-hydroxy phenyl) after pyrazoles, chlorella growth is suppressed after 9 days in cultivation, and growth inhibition ratio reaches 44.3%(Fig. 1).

Claims

1. polygalacturonase suppresses the application in algal grown, it is characterized in that:

(1) when chlorella algae liquid absorbancy reaches 0.25, start to add polygalacturonase and (2,4,6-trihydroxy-phenyl)-4-(4-hydroxy phenyl) pyrazoles;

(2) polygalacturonase adds dosage is 640 mg/L;

(3) (2,4,6-trihydroxy-phenyl)-4-(4-hydroxy phenyl) to add dosage be 32-96 mg/L to pyrazoles

(4) the input cycle is 9 days.