CN104940666A - Pharmaceutical composition having anti-hypoxia effect and preparation method - Google Patents
Pharmaceutical composition having anti-hypoxia effect and preparation method Download PDFInfo
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Abstract
The invention relates to a natural pharmaceutical composition having an anti-hypoxia effect and a preparation method, wherein the pharmaceutical composition contains the following components in parts by weight: 5-20 parts of total saponins of ginseng, 10-40 parts of general flavones of ampelopsis grossedentata, and 15-45 parts of bamboo extract. The preparation method comprises the following steps of: extracting sinseng by using ethanol refluxing, enabling the extract to pass through a D-101 macro-porous absorption resin column, recycling ethanol in eluant, drying to obtain the total saponins of ginseng, extracting ampelopsis grossedentata by using ethanol refluxing, enabling the extract to pass through a AB-8 macro-porous absorption resin column, recycling ethanol in eluant, drying to obtain the general flavones of ampelopsis grossedentata, aqueously extracting bamboos, separating out filtrate, concentrating the filtrate, and drying to obtain the bamboo extract. The active effect in the invention is that the natural pharmaceutical composition having the anti-hypoxia effect is extracted from plant extracts; the characteristics of being multiple in target point and effect in traditional Chinese medicines are embodied; the effects of treating both symptoms and root causes can be realized; and the natural pharmaceutical composition has obvious breakthrough and improvement in terms of curative effect and safety.
Description
Technical field
The invention belongs to medical art, be specifically related to a kind of natural drug composition and the preparation method with resisting oxygen lack.
Technical background
Oxygen is the essential condition that the mankind and many biologies are depended on for existence.Oxygen needed for body vital movement is then called hypoxia or anoxia when can not obtain the state of sufficient supply.Anoxia will cause organism metabolism, function, even morphosis generation abnormal change.Serious or long term hypoxia can bring serious harm to body, finally can cause the important organs such as the body heart, brain due to energy under-supply and dead.Oxygen and hypoxia are the important topics of life sciences basic theories.The formation of hypoxia can be divided three classes: the first kind is that external environment oxygen content reduces, and makes normal physiological activity process can not absorb enough oxygen, as the anoxia that the special environments such as plateau, diving and aviation cause; Equations of The Second Kind refers to because disease etc. causes extraneous normal oxygen amount fully not arrive in body, causes the anoxia of the heart, brain and respiratory system etc.; 3rd class is body activities requisite oxygen consumption, has exceeded the physiology ability of mobilization, causes relative oxygen supply not enough, is common in strenuous exercise and the amount of transfiniting work.Long-term hypoxia is the important hidden danger be detrimental to health, severe patient even can threat to life, Pulmonary Diseases (pneumonia, pulmonary edema etc.) can be there is, the ventilation of thorax illness (hydrothorax, pneumothorax etc.) and the lung caused by other some diseases (as hypothyroidism, metabolic alkalosis etc.) and (or) dysfunction of ventilation, therefore, medicine or the health food of studying anoxia enduring have wide market development prospect.
Meanwhile, being open to traffic in recent years along with Qinghai-Tibet Railway, the quantity of the visitor that enters into Tibet constantly rises, and acute high altitude reaction becomes a large obstacle of tourist industry.Meanwhile, the continuous propelling of building along with Tibetan area economy, the crowd of work of more and more entering into Tibet needs altitude acclimatization.In addition, also have the soldier on a large amount of army limit, live in high altitude localities throughout the year, in the urgent need to the functional food of anoxia enduring and resisting fatigue, auxiliary its adapts to the severe extreme climate in plateau as early as possible.So exploitation high-quality, the functional food efficiently with resisting oxygen lack have very wide market prospect.
The present invention is exactly by a kind of natural drug composition with resisting oxygen lack found from natural plants or extract.Said composition becomes composition of natural products by effective ingredient innovative combination, embodies the feature of Chinese medicine " Mutiple Targets, manifold effect ", have the effect for the treatment of both the principal and secondary aspects of a disease, to there being significant breakthrough and raising from efficacy and saferry.
Radix Ginseng total saponins is Araliaceae Radix Ginseng
panax ginsengthe root of C.A .Mey. is through extraction, purification, the total saponins be processed into.There is raising immunity of organisms, improve cardiovascular and cerebrovascular vessel blood supply insufficiency, regulate the effects such as central nervous system, resisting fatigue, slow down aging.Rivers and mountains reports Radix Ginseng total saponins by suppressing high concentration of glutamate and K
+the calcium concentration caused increases, and increases membrane fluidity, scavenging free radicals, extend the time-to-live of old and feeble neurocyte, ischemia resisting associated with hydrocephalus, the approach such as anti-apoptotic improve ischemia resisting damaging action (Xuzhou Medical College's journal of neurocyte, 2003,23 volume the 3rd phases, 209).Dong Li reports Radix Ginseng total saponins Wei resisting oxygen lack, obviously can extend the time-to-live of white mice under low pressure or normobaric hypoxia condition, the increase of erythrocyte and content of hemoglobin under anaerobic condition, so body hypoxia endurance (Chinese public health can be improved, 2006,22 volumes the 2nd phase, 198).
Ampelopsis grossedentata is the new resource food of national regulation.Ampelopsis grossedentata total flavone is from Vitaceae ampelopsis Ampelopsis grossedentata (Ampelopsis grossedentata)
ampelopsis Grossedenta(Hand-Mazz.) extraction purification and obtaining in W.T.Wa.In Ampelopsis grossedentata young stem and leaf, the content of total flavones is up to 43.4%-45.52%.Chromocor compound wide variety in Ampelopsis grossedentata, has dihydromyricetin, Quercetin, Taxifolin, kaempferol, Ampelopsis grossedentata element, myricetrin, hesperetin, apiolin etc.Wherein dihydromyricetin is the main active in Ampelopsis grossedentata.Research shows, Ampelopsis grossedentata total flavone toxicity is little, mouse stomach administration, maximum tolerated dose is that 22.5g/kg(clock is just virtuous, the experimentation of total Flavone of Ampelopsis grossedentata from Guangxi in Liver Protection, Guangxi science, 2002,1st phase, 57), and have physiologically active widely, its antioxidation can scavenging activated oxygen, the oxidative damage in body can be suppressed, the regulating action scalable human immunologic function of immunologic function; Reduce blood pressure and blood lipoid effect to can be used for preventing and treating the diseases such as diabetes, atherosclerosis, hyperlipidemia; There is the effects such as hepatoprotective, antithrombotic, antiviral, antiinflammatory simultaneously.
Folium Bambosae flavone be grass family Phyllostachys plant Ph. meyeri (
phyllostachys meyeri) through the total flavones extracting, concentrate, purification obtains.Main component is orientin, Lutonaretin, apigenin-8-C-glucoside and Saponaretin etc.Except there is effect of free radical resisting, antioxidation, antiinflammatory, enhancing immunity, slow down aging, also there is significant anoxia enduring, resisting fatigue and anti-stress effect.Shenyang Pharmaceutical University pays the systematic study (new Chinese medicine and clinical pharmacology, 2005,16(2) that dawn, spring etc. carried out: 100-102; Chinese patent medicine, 2006,28(4): 558-560; Modern food and medicine magazine, 2006,16(1): 18-21; Research and development of natural products, 2006,18: 214-218; Traditional Chinese Medicine University Of Guangzhou's journal, 2006,23(6): 492-495; Combination of Chinese and Western medicine cardiovascular and cerebrovascular disease magazine, 2007,5(2): 125-126) show, the mouse survival time under Folium Bambosae flavone energy significant prolongation normobaric hypoxia condition, significantly improve the hypoxia-bearing capability of whole animal; Obvious protective effect is produced to the cardiac muscle of acute ischemia, anoxia, its mechanism of action with activities of antioxidant enzymes in its antagonism radical damage, reinforcement, reduce myocardial oxygen consumption, to reduce myocardial infarct size relevant.
Before the present invention completes, also do not find that compositions that Radix Ginseng total saponins, Ampelopsis grossedentata total flavone, Folium Bambosae extract form has the report of resisting oxygen lack.
Summary of the invention
The object of this invention is to provide a kind of natural drug composition and the preparation method with resisting oxygen lack,
Activity (having treatment, the health-care effect) composition that the natural drug composition with resisting oxygen lack of the present invention comprises and each component content ratio are: Radix Ginseng total saponins 5-20 weight portion, Ampelopsis grossedentata total flavone 10-40 weight portion, Folium Bambosae extract 15-45 weight portion.
The active component that the present invention comprises and optimum amount ratio are: Radix Ginseng total saponins 8 weight portion, Ampelopsis grossedentata total flavone 20 weight portion, Folium Bambosae extract 30 weight portion.
Instructions of taking and dose: oral, adult's 1-2g/ day.
The preparation process with the natural drug composition of resisting oxygen lack of the present invention is:
1 gets ginseng crude drug with 50-95% alcohol reflux 2-3 time, and each 2-3 hour, filters, filtrate recycling ethanol, obtain ethanol extraction, ethanol extraction, by the D-101 macroporous adsorptive resins of anticipating, first uses deionized water eluting, discard eluent, continue and use 50-90% ethanol elution, collect eluent, reclaim ethanol, drying, obtains Radix Ginseng total saponins;
2 get Ampelopsis grossedentata medical material 50-90% alcohol reflux 2-3 time, each 2-3 hour, filter, filtrate recycling ethanol, obtains ethanol extraction, the AB-8 macroporous adsorptive resins of ethanol extraction by anticipating, first use deionized water eluting, discard eluent, then use 10-25% ethanol elution, discard eluent, continue and use 60-90% ethanol elution, collect eluent, reclaim ethanol, drying, obtains Ampelopsis grossedentata total flavone;
3 get Folium Bambusae medical material 5-9 water extraction doubly 2-3 time, each 1-2 hour, and filter, filtrate concentrates, dry, obtains Folium Bambosae extract;
The 4 Radix Ginseng total saponins 50-200 weight portions getting step 1 gained, get the Ampelopsis grossedentata total flavone 100-400 weight portion of step 2 gained, get the Folium Bambosae extract 150-450 of step 3 gained, mix homogeneously, must have the natural drug composition of resisting oxygen lack; Add appropriate amount of auxiliary materials, make granule, tablet, soft sweet or other traditional oral preparations.
The present invention is from effective substance, through repetition test, select the raw material that can be used for health food and new resource food of three kinds of Ministry of Public Health regulations such as Radix Ginseng, Ampelopsis grossedentata, Folium Bambusae, utilize the Radix Ginseng total saponins, Ampelopsis grossedentata total flavone, the Folium Bambosae extract prescription that therefrom extract respectively, so this prescription composition is very safe, for the preparation of contributing to the health food or the medicine that improve anoxia endurance, without any side effects.When choosing each raw material of the present invention and carrying out combination collocation, give full play to their respective effects, and through reasonably combined generation trade-off effect.Except consideration ischemia resisting, except anoxia material base, the present invention more outstanding contributions is the prevention and therapy of answering acute inflammatory seep that considers because ischemia, anoxia cause to cardiovascular and cerebrovascular vessel tissue on prescription composition.First by the Ampelopsis grossedentata total flavone with antiinflammatory with there is ischemia resisting, anoxia enduring, the Radix Ginseng total saponins of antifatigue and increasing immune power and Folium Bambosae extract prescription, this compositions has significantly different from Western medicine market with the single target spot of anoxia enduring ischemia; Simultaneously contribute to improving compared with anoxia endurance Chinese patent medicine with existing at present, have activity more definite, effective substance is clearer, and prescription forms and has obvious characteristic, and this is outstanding contributions of the present invention.
Radix Ginseng total saponins has the effects such as vasodilator, anoxia enduring, cardioprotection ischemical reperfusion injury, protection cerebral ischemia, resisting fatigue and tonification; Ampelopsis grossedentata total flavone has cardiac nutrition, the effect of blood vessel dilating, exudation inflammation, antioxidation, change hemorheology index; Folium Bambosae extract has free radical resisting, antioxidation, resisting fatigue, shields to the myocardial cell of Hypoxia and ischemia.These chemical compositions are collaborative, Mutiple Targets, multipath effect mutually, fundamentally mediator's body's hypoxia tolerance comprehensively, improves physical condition.The present invention is by the protective effect on anaerobic condition hyponeural system system, the protective effect of cardiovascular system, the impact of human body energy metabolism; by improving antioxidant ability of organism, to the apoptotic antagonism of hypoxia inducible, reaching and contributing to improving body anoxia endurance.There is obvious novelty and technological progress.Another contribution of the present invention is, proves that the more single Radix Ginseng total saponins of the present composition, Ampelopsis grossedentata total flavone and Folium Bambosae extract have the activity of stronger anoxia enduring, embody the synergism feature of Chinese medicine compound and effective substance through pharmacodynamic experiment.
For realizing above technical scheme, the content of total saponins, the content of total flavones measure by the following method:
The content assaying method of total saponins
The preparation precision of reference substance solution takes ginsenoside Rb1's reference substance 10.0mg, puts in 10mL measuring bottle, adds anhydrous alcohol solution and be diluted to scale, shaking up, to obtain final product.
Test sample 50.0mg is got in the preparation of need testing solution, puts in 50mL measuring bottle, adds anhydrous alcohol solution and is diluted to scale, shaking up, to obtain final product.
Algoscopy precision measures reference substance solution and each 50 μ l of need testing solution, and putting in tool plug test tube respectively, take dehydrated alcohol as blank, respectively evaporate to dryness; Respectively add 5% vanillin-glacial acetic acid solution 0.2ml, perchloric acid 0.8ml, airtight, to shake up in rearmounted 70 DEG C of water-baths 20 minutes, taking-up is chilled to room temperature, add ethyl acetate 5ml, after shaking up, according to spectrophotography (" Chinese Pharmacopoeia " version in 2000 annex V B), measure trap respectively at wavelength 560nm place, calculate.
Determination Method of Flavone Content
The preparation of reference substance solution: precision takes control substance of Rutin 10.0mg, puts in 50mL measuring bottle, adds anhydrous alcohol solution and is diluted to scale, shaking up, to obtain final product.
The preparation of standard curve: get reference substance 10mg, be mixed with the solution of every 1mL containing 0.2mg with dehydrated alcohol, get 0.1mL, 0.2 mL successively, 0.4 mL, 0.8 mL, 1.6mL are placed in 10mL volumetric flask, precision adds 1%AlCl
3solution 3mL, uses dehydrated alcohol standardize solution, and shake up rear room temperature and place 30min, retinue is blank, and survey trap in wavelength 370nm place, take content as abscissa, trap is vertical coordinate drawing standard curve.
Test sample 10.0mg is got in the preparation of need testing solution, puts in 50mL measuring bottle, adds anhydrous alcohol solution and is diluted to scale, shaking up, to obtain final product.
Algoscopy precision measures need testing solution 0.3mL, and put in 10mL measuring bottle, precision adds 1%AlCl
3solution 3mL, uses dehydrated alcohol standardize solution, and shake up rear room temperature and place 30min, retinue is blank.According to spectrophotography, measure trap respectively at wavelength 370nm place, calculate.
The compositions that another object of the present invention there is provided the Radix Ginseng total saponins of said extracted, Ampelopsis grossedentata total flavone and Folium Bambusae total flavones composition contributes to improving the application in the medicine of anoxia endurance, health food and food in preparation.Because the present invention makes public for the first time the compositions be made up of Radix Ginseng total saponins, Ampelopsis grossedentata total flavone and Folium Bambosae extract, there is the effect contributing to improving anoxia endurance; therefore; by this compositions separately or coordinate with other active constituent or adjuvant and make medicament; as long as this medicament is used for contributing to improving anoxia endurance, all belong to protection scope of the present invention.Compositions of the present invention, when making any one dosage form, all has the effect of anoxia enduring.）
The compositions (be called for short compositions, example 4 is prepared and obtains) be made up of Radix Ginseng total saponins, Ampelopsis grossedentata total flavone and Folium Bambosae extract has resisting oxygen lack, is confirmed by following pharmacodynamic experiment.
The various raw materials that the present invention needs provide by Jilin Modern Chinese Medicine Engineering Research Center Co., Ltd..Sodium nitrite analytical pure, sodica calx, lactic acid assay kit builds up Bioengineering Research Institute by Nanjing and produces, and lot number is 20130802.Positive reference substance medicine Radix Rhodiolae ginseng Qi capsule (lot number: 20131201, Sichuan Mai Liruo development in science and technology Co., Ltd).
ICR mice 160, body weight 18 ~ 22g, the high-new Experimental Animal Center in Changchun provides, and laboratory animal production licence number is 2013-0010.
Mice 80 is got in each experiment, is divided into 8 groups, often organizes 10.If 6 test group, 1 blank group and 1 positive controls.The dosage of present composition test group is respectively 50mg/kg, 100mg/kg, 200mg/kg; The dosage of Radix Ginseng total saponins, Ampelopsis grossedentata total flavone and Folium Bambosae extract is 200mg/kg; Blank group gives distilled water; Positive controls dosage is 300mg/kg.Test each group of gavage to sample, every day 1 time, continuous 30d, gavage volume is 0.1ml/10g body weight.
1, this compositions is on the impact of mice oxygen deficit tolerance time-to-live
1h after last gives sample, puts into the 250ml port grinding bottle (1 every bottle) filling 5g sodica calx respectively, seals by each group of mice, with respiratory arrest for dead indication, observe the mouse survival time.Experimental result is in table 1.
This compositions of table 1 is on the impact of mice oxygen deficit tolerance time-to-live
Compare with blank group, * P < 0.05, * * P < 0.01
As shown in Table 1, the basic, normal, high three dosage groups of the present composition all can extend the time-to-live of mice, and the oxygen deficit tolerance time-to-live is compared with blank group, and high, middle dosage group has significant difference.And the high dose group mouse survival time is longer than low dosage and middle dosage group mice.Experimental data shows simultaneously, and the more single Radix Ginseng total saponins of high, the middle dosage group of the present composition, Ampelopsis grossedentata total flavone and Folium Bambosae flavone have stronger pharmacologically active.
2, the present composition is on the impact of mice sodium nitrite poisoning time-to-live
1 h after last gives sample, each treated animal presses 240mg/kg body weight dose lumbar injection sodium nitrite, observes animal survival time.Experimental result is in table 2.
Table 2 present composition is on the impact of mice sodium nitrite poisoning time-to-live
Compare with blank group, * P < 0.05, * * P < 0.01
As shown in Table 2, compared with blank group, the present composition 3 dosage groups all obviously can extend the mice sodium nitrite poisoning time-to-live, have significant difference.The more single Radix Ginseng total saponins of high, the middle dosage group of the present composition, Ampelopsis grossedentata total flavone and Folium Bambosae extract have stronger activity.
3, the present composition is on the impact of Acute cerebral ischemia in mice the survival time under hypoxic condition
1h after last gives sample, each treated animal from cervical region by only breaking end, immediately by stopwatch record mice broken end after to dwell time of breathing of dehiscing.Experimental result is in table 3.
Table 3 present composition is on the impact of Acute cerebral ischemia in mice the survival time under hypoxic condition
Compare with blank group, * P < 0.05, * * P < 0.01
From table 3, high, the middle dosage group of the present composition can obviously extend Acute cerebral ischemia in mice the survival time under hypoxic condition.The more single Radix Ginseng total saponins of high, the middle dosage group of the present composition, Ampelopsis grossedentata total flavone and Folium Bambosae extract have stronger activity.
4, the present composition is on the impact of blood lactase acid after mouse movement
Take a blood sample after mice last gives sample 30min, then do not bear a heavy burden to take out after temperature is the water went swimming 10min of 30 DEG C and again take a blood sample immediately, finally rest 20min the 3rd blood sampling.By the Serum lactic acid content of kit measurement 3 time points, and be calculated as follows lactic acid area under curve.Experimental result is in table 4.
Blood lactase acid area under curve=5 × (the blood lactase acid value of the rear rest 20min of blood lactase acid value+2 × swimming of the rear 0min of front blood lactase acid value+3 × swimming that swims)
Table 4 present composition is on the impact of blood lactase acid after mouse movement
Compare with blank group, * P < 0.05, * * P < 0.01
From table 4, high, the middle dosage group of the present composition obviously can reduce blood lactase acid area under curve content after mouse movement.Thus improve the energy supply level of body carbohydrate metabolism system, reduce the ratio of protein metabolism energy supply, thus improve hypoxia-bearing capability.Meanwhile, test data shows that the more single Radix Ginseng total saponins of the present composition, Ampelopsis grossedentata total flavone and Folium Bambosae extract have stronger activity.
Detailed description of the invention
Embodiment 1(granule)
1, Radix Ginseng 10kg is got, with 10 times amount 60% alcohol reflux 2 times, each 2 hours, filter, filtrate recycling ethanol, obtains ethanol extraction, add water to every 1ml containing 0.8g medical material, stir evenly, by the D-101 macroporous resin column of anticipating, first use deionized water eluting 5 times of column volumes, discard eluent, continue with 70% ethanol elution, 4 times of column volumes, collect eluent, reclaim ethanol, dry, obtain Radix Ginseng total saponins.
With ginsenoside Rb
1for reference substance, adopt determined by ultraviolet spectrophotometry, the content of total saponins is 83.9%.
2, Ampelopsis grossedentata 20kg is got, with 70% alcohol reflux 2 times, each 3 hours, filter, filtrate recycling ethanol, obtains ethanol extraction, add water to every 1ml containing 1.0g medical material, stir evenly, by the AB-8 macroporous resin column of anticipating, first use deionized water eluting 3 times of column volumes, discard eluent, then use 20% ethanol elution, 3 times of column volumes, discard eluent, continue with 80% ethanol elution, 5 times of column volumes, collect eluent, reclaim ethanol, dry, obtain Ampelopsis grossedentata total flavone; Be reference substance with rutin, utilize determined by ultraviolet spectrophotometry, the content of total flavones is 85.7%.
3, Folium Bambusae 20kg, Folium Bambusae 5-9 water extraction doubly 2-3 time, each 1-2 hour, filters, and filtrate concentrates, and drying, obtains Folium Bambosae extract;
4, take the Radix Ginseng total saponins 50g that step 1 obtains, step 2 obtains Ampelopsis grossedentata total flavone 400g, and step 3 obtains Folium Bambusae total flavones 150g, and medical starch is appropriate, mix homogeneously, granulates, and dry, granulate, incapsulates, and makes 1000, to obtain final product.
Embodiment 2 (tablet)
The extracting method of Radix Ginseng total saponins, Ampelopsis grossedentata total flavone, Folium Bambusae total flavones is with embodiment 1.
Take Radix Ginseng total saponins 100g, Ampelopsis grossedentata total flavone 100g, Folium Bambusae total flavones 450g, medical starch is appropriate, mix homogeneously, granulates, and dry, granulate, tabletting, makes 1000, to obtain final product.Oral, twice daily, each 1-2 sheet.
Embodiment 3(soft sweet dosage form)
1, the extracting method of Radix Ginseng total saponins, Ampelopsis grossedentata total flavone, Folium Bambusae total flavones is with embodiment 1.
2, by glucose 2000g, white sugar 150g, citric acid 80g, sodium citrate 8g, modified starch 80g, join in 1 times of water gaging, heating makes it all dissolve, and crosses 100 mesh sieves, for subsequent use; By gelatin 800g, join in 8 times of water gagings, heating makes it all dissolve, mix with the solution after above-mentioned sieving, add Radix Ginseng total saponins 8g, Ampelopsis grossedentata total flavone 20g, Folium Bambosae extract 30g, heating boils, let cool, releasing agent is made, coating release agent on grinding tool, cast with the modified starch of 1:1 and the mixture of Oleum Arachidis hypogaeae semen, drying 1 hour at 40 DEG C, the demoulding, subpackage, obtains 1000 soft sweets.Chew in oral cavity, every day 3 times, each 1-2 block.
Claims (3)
1. have a pharmaceutical composition for resisting oxygen lack, it is characterized in that, the content ratio of the active component comprised and each composition is: Radix Ginseng total saponins 5-20 weight portion, Ampelopsis grossedentata total flavone 10-40 weight portion, Folium Bambosae extract 15-45 weight portion.
2. a kind of pharmaceutical composition with resisting oxygen lack according to claim 1, it is characterized in that, the content ratio of the active component comprised and each composition is: Radix Ginseng total saponins 8 weight portion, Ampelopsis grossedentata total flavone 20 weight portion, Folium Bambosae extract 30 weight portion.
3. an a kind of preparation method with the pharmaceutical composition of resisting oxygen lack as claimed in claim 1 or 2, the steps include:
(1) get ginseng crude drug with 50-95% alcohol reflux 2-3 time, each 2-3 hour, filters, filtrate recycling ethanol, obtain ethanol extraction, ethanol extraction, by the D-101 macroporous adsorptive resins of anticipating, first uses deionized water eluting, discard eluent, continue and use 50-90% ethanol elution, collect eluent, reclaim ethanol, drying, obtains Radix Ginseng total saponins;
(2) get Ampelopsis grossedentata medical material 50-90% alcohol reflux 2-3 time, each 2-3 hour, filter, filtrate recycling ethanol, obtains ethanol extraction, the AB-8 macroporous adsorptive resins of ethanol extraction by anticipating, first use deionized water eluting, discard eluent, then use 10-25% ethanol elution, discard eluent, continue and use 60-90% ethanol elution, collect eluent, reclaim ethanol, drying, obtains Ampelopsis grossedentata total flavone;
(3) get Folium Bambusae medical material 5-9 water extraction doubly 2-3 time, each 1-2 hour, filter, filtrate concentrates, dry, obtains Folium Bambosae extract;
(4) the Radix Ginseng total saponins 5-20 weight portion getting step (1) gained, the Ampelopsis grossedentata total flavone 10-40 weight portion getting step (2) gained, the Folium Bambosae extract 15-45 of step (3) gained is got, mix homogeneously, must have the natural drug composition of resisting oxygen lack; Add appropriate amount of auxiliary materials, make granule, tablet, soft sweet or other traditional oral preparations.
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