CN104940666B - A kind of natural drug composition and preparation method with resisting oxygen lack - Google Patents
A kind of natural drug composition and preparation method with resisting oxygen lack Download PDFInfo
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Abstract
A kind of natural drug composition and preparation method with resisting oxygen lack, pharmaceutical composition therein include the parts by weight of general ginsenoside 5 20, the parts by weight of Ampelopsis grossedentata total flavone 10 40, the parts by weight of bamboo extractive 15 45.Preparation process is：Ginseng crude drug extracts extract by the large pore resin absorption columns of D 101 with alcohol reflux, reclaims ethanol in eluent, dry general ginsenoside.Ampelopsis grossdentata medicinal material alcohol reflux extracts extract by the large pore resin absorption columns of AB 8, reclaims ethanol in eluent, dry Ampelopsis grossedentata total flavone.Leaf of bamboo medicinal material water extraction, filtrate is isolated, filtrate concentration, dries, obtains bamboo extractive.The positive effect of the present invention is a kind of natural drug composition with resisting oxygen lack extracted from natural plants.The characteristics of embodying Chinese medicine " Mutiple Targets, manifold effect ", there is the effect treated both principal and secondary aspect of disease, have significant breakthrough from efficacy and saferry and improve.
Description
Technical field
The invention belongs to pharmaceutical technology field, and in particular to a kind of natural drug composition and system with resisting oxygen lack
Preparation Method
Technical background
The essential condition that oxygen is the mankind and many biologies are depended on for existence.Oxygen needed for body vital movement can not obtain abundance
Supply state when then be referred to as hypoxemia or anoxic.Anoxic will cause organism metabolism, function, even morphosis that abnormal change occurs
Become.Serious or long term hypoxia can bring serious harm to body, can finally cause the important organs such as the body heart, brain to be supplied due to energy
Should be insufficient and dead.Oxygen and hypoxemia are the important topics of life science basic theories.The formation of hypoxemia can be divided into three classes：The first kind
It is the reduction of external environment oxygen content, preventing normal physiological activity process, such as plateau, diving and aviation are special from absorbing enough oxygen
Anoxic caused by environment；Second class refers to, because disease etc. causes extraneous normal oxygen amount to substantially arrive in body, cause the heart, brain
With the anoxic of respiratory system etc.；3rd class is zmount of oxygen consumption needed for body activities, has exceeded the physiology ability of mobilization, causes relative oxygen
Supply deficiency, it is common in strenuous exercise and the amount of transfiniting work.Long-term hypoxemia is the important hidden danger being detrimental to health, and severe patient is very
Extremely can threat to life, may occur in which Pulmonary Diseases (pneumonia, pulmonary edema etc.), thorax illness (pleural effusion, pneumothorax etc.) and other one
The ventilation of lung caused by a little diseases (such as hypothyroidism, metabolic alkalosis etc.) and (or) dysfunction of ventilation, therefore,
The medicine or health food for studying resist oxygen lack have wide market development prospect.
Meanwhile being open to traffic recently as Qinghai-Tibet Railway, the quantity for the visitor that enters into Tibet is constantly soaring, and acute high altitude reaction turns into
One big obstacle of tourist industry.Meanwhile with the continuous propulsion of Tibetan area economy construction, the crowd's needs for work of more and more entering into Tibet
Altitude acclimatization.In addition, the soldier on also a large amount of army sides, lives in high altitude localities throughout the year, there is an urgent need to anti anoxia and antifatigue
Functional food, aid in it to adapt to the severe extreme climate in plateau as early as possible.So exploitation is high-quality, efficiently has resist oxygen lack
The functional food of effect has very wide market prospects.
The present invention is exactly a kind of natural drug group with oxygen lack resistant function that will be found or extract from natural plants
Compound.Said composition is to embody Chinese medicine " Mutiple Targets, manifold effect " into composition of natural products by active ingredient innovative combination
Feature, there is the effect treated both principal and secondary aspect of disease, to have significant breakthrough from efficacy and saferry and improve.
General ginsenoside is extracted Araliaceae ginseng Panax ginseng C.A.Mey. root, purifying, processing
Manufactured total saposins.With improve immunity of organisms, improve cardiovascular and cerebrovascular blood supply insufficiency, regulation central nervous system, it is antifatigue,
Anti-aging etc. acts on.Rivers and mountains reports general ginsenoside by suppressing high concentration of glutamate and K+Caused calcium concentration increases,
Increase membrane fluidity, remove free radical, extend the time-to-live of aging nerve cell, anti-Neurons Against Cerebral Ischemia oedema, anti-apoptotic
Etc. the anti-ischemic injuries effect (Xuzhou Medical College's journal, the phase of volume 2003,23 the 3rd, 209) that approach improves nerve cell.Dong Li Weis
Reporting general ginsenoside has oxygen lack resistant function, can be obviously prolonged the time-to-live of small white mouse under the conditions of low pressure or normobaric hypoxia,
The increase of red blood cell and content of hemoglobin under anaerobic condition, thus can improve body hypoxia endurance (Chinese public health,
The phase of volume 2006,22 the 2nd, 198).
Ampelopsis grossdentata is the new resource food of national regulation.Ampelopsis grossedentata total flavone is planted from Vitaceae Ampelopsis
Extraction purification in thing ampelopsis grossdentata (vine tea) Ampelopsis Grossedenta (Hand-Mazz.) W.T.Wa and obtain.Aobvious tooth
The content of general flavone is up to 43.4%-45.52% in porcelain ampelopsis young stem and leaf.Chromocor compound species in ampelopsis grossdentata is more
Sample, there are dihydromyricetin, Quercetin, texifolin, Kaempferol, ampelopsis grossdentata element, myricetrin, hesperetin, apiolin etc..Its
Middle dihydromyricetin is the main active in ampelopsis grossdentata.Research shows, Ampelopsis grossedentata total flavone small toxicity, mouse
Gastric infusion, maximal tolerance dose are 22.5g/kg (Zhong Zhengxian, the experimental study of total Flavone of Ampelopsis grossedentata from Guangxi in Liver Protection, Guangxi section
Learn, the 2002, the 1st phase, 57), and there is extensive physiologically active, its antioxidation can scavenging activated oxygen, machine can be suppressed
Internal oxidative damage, the adjustment effect of immunologic function can adjust human immunologic function；Blood pressure and blood lipoid effect is reduced to can be used for preventing
Control the diseases such as diabetes, atherosclerosis, high fat of blood；There is the effect such as protect liver, antithrombotic, antiviral, anti-inflammatory simultaneously.
Bamboo-leaves flavones are that grass family Phyllostachys plant Ph. meyeri (Phyllostachys meyeri) is extracted, concentrate, be pure
Change obtained general flavone.Main component is orientoside, Lutonaretin, Vitexina and Saponaretin etc..Except with Green Tea Extract, anti-
Outside the effect of oxidation, anti-inflammatory, enhancing are immune, anti-aging, also with significant resist oxygen lack, antifatigue and anti-stress effect.Shen
Positive pharmaceutical university pays system research (new Chinese medicine and clinical pharmacology, 2005,16 (2) of the progress such as spring dawn：100-102；It is middle into
Medicine, 2006,28 (4)：558-560；Modern food and medicine magazine, 2006,16 (1)：18-21；Research and development of natural products,
2006,18：214-218；Traditional Chinese Medicine University Of Guangzhou's journal, 2006,23 (6)：492-495；Combination of Chinese tradiational and Western medicine cardiovascular and cerebrovascular diseases are miscellaneous
Will, 2007,5 (2)：125-126) show, the mouse survival time that bamboo-leaves flavones can significantly extend under the conditions of normobaric hypoxia, significantly
Improve the anti-anoxia ability of whole animal；To acute ischemia, anoxic cardiac muscle produce obvious protective function, its mechanism of action and its
Activities of antioxidant enzymes, reduction myocardial oxygen consumption, reduction myocardial infarct size are relevant in confrontation radical damage, reinforcement.
Before the present invention completes, the combination of general ginsenoside, Ampelopsis grossedentata total flavone, bamboo extractive composition is not found also
Thing has the report of oxygen lack resistant function.
The content of the invention
It is an object of the invention to provide a kind of natural drug composition and preparation method with oxygen lack resistant function,
The activity (having treatment, health-care efficacy) that is included of the natural drug composition with oxygen lack resistant function of the present invention into
Divide and each component content ratio is：General ginsenoside 5-20 parts by weight, Ampelopsis grossedentata total flavone 10-40 parts by weight, the leaf of bamboo carry
Take thing 15-45 parts by weight.
The active component of the invention included and optimum amount ratio are：The parts by weight of general ginsenoside 8, ampelopsis grossdentata are total
The parts by weight of flavones 20, the parts by weight of bamboo extractive 30.
Instructions of taking and dose：Orally, it is grown up 1-2g/ days.
The preparation process of the natural drug composition with resisting oxygen lack of the present invention is：
1 takes ginseng crude drug to be extracted 2-3 times with 50-95% alcohol refluxs, each 2-3 hours, filtering, filtrate recycling ethanol,
Ethanol extract, ethanol extract by the D-101 large pore resin absorption columns anticipated, be first washed with deionized water it is de-,
Eluent is discarded, after with 50-90% ethanol elutions, eluent is collected, reclaims ethanol, dry, obtain general ginsenoside；
2 take ampelopsis grossdentata medicinal material 50-90% alcohol refluxs to extract 2-3 times, each 2-3 hours, filtering, filtrate recovery
Ethanol, obtains ethanol extract, and ethanol extract is first washed with deionized water by the AB-8 large pore resin absorption columns anticipated
It is de-, discard eluent, then with 10-25% ethanol elutions, discard eluent, after with 60-90% ethanol elutions, collect eluent,
Ethanol is reclaimed, dries, obtains Ampelopsis grossedentata total flavone；
3 take the water of 5-9 times of leaf of bamboo medicinal material to extract 2-3 times, each 1-2 hours, filtering, filtrate concentration, dry, obtain the leaf of bamboo
Extract；
4 Ampelopsis grossedentata total flavones for taking the general ginsenoside 50-200 parts by weight of step 1 gained, taking step 2 gained
100-400 parts by weight, the bamboo extractive 150-450 for taking step 3 gained, it is well mixed, obtains with the natural of resisting oxygen lack
Pharmaceutical composition；Appropriate amount of auxiliary materials is added, granule, tablet, soft sweets or other traditional oral preparations are made.
The present invention from effective substance, by repetition test, selected ginseng, ampelopsis grossdentata, leaf of bamboo etc. three
Available for health food and the raw material of new resource food as defined in kind of the Ministry of Public Health, using the general ginsenoside therefrom extracted respectively,
Ampelopsis grossedentata total flavone, bamboo extractive prescription, so the composition is very safe, anoxic is favorably improved for preparing
The health food or medicine of tolerance, it is without any side effects.When each raw material for choosing the present invention is combined collocation, fully
The effect of having played each of which, and through reasonably combined generation trade-off effect.Except the anti-ischemic of consideration, anoxic thing in composition
Matter basis is outer, and more outstanding contributions of the invention are to consider because ischemic, anoxic answer acute inflammation to caused by cardiovascular and cerebrovascular tissue
The prevention and treatment that disease is oozed out.First by the Ampelopsis grossedentata total flavone with anti-inflammatory with anti-ischemic, anti anoxia, antifatigue
The general ginsenoside and bamboo extractive prescription of strengthen immunity, this composition have the single target spot of anti anoxia ischemic with the market
Western medicine have it is obvious different；Simultaneously with present it is existing be favorably improved anoxia endurance Chinese patent drug compared with, it is active
More precisely, effective substance becomes apparent from, and composition has obvious characteristic, and this is the outstanding contributions of the present invention.
General ginsenoside have vasodilator, anti anoxia, cardioprotection ischemical reperfusion injury, protection cerebral ischemia,
Antifatigue and help etc. acts on；Ampelopsis grossedentata total flavone has a cardiac nutrition, expansion blood vessel, it is impervious go out inflammation, it is anti-oxidant, change
Become the effect of hemorheology index；Bamboo extractive has Green Tea Extract, anti-oxidant, antifatigue, to Hypoxia and ischemia cardiac muscle
Cell shields.These chemical compositions mutually cooperate with, the effect of Mutiple Targets, multipath, fundamentally adjust human organism comprehensively
Hypoxia-bearing capability, improve physical condition.The present invention passes through the protective effect united to anaerobic condition hyponeural system, cardiovascular system
Protective effect, the influence of human body energy metabolism of system, by improving antioxidant ability of organism, pair to hypoxia inducible Apoptosis
Anti- effect, reach and be favorably improved body anoxia endurance.With obvious innovative and technological progress.The present invention another
Contribution is, the present composition more single general ginsenoside, Ampelopsis grossedentata total flavone and the leaf of bamboo are proved through pharmacodynamic experiment
Extract has the activity of stronger anti anoxia, embodies Chinese medicine compound prescription and the synergy feature of effective substance.
To realize above technical scheme, the content of total saposins, the content of general flavone determine by the following method：
The content assaying method of total saposins
The preparation precision of reference substance solution weighs ginsenoside Rb1 reference substance 10.0mg, puts in 10mL measuring bottles, adds anhydrous second
Alcohol dissolves and is diluted to scale, shakes up, produces.
The preparation of need testing solution takes test sample 50.0mg, puts in 50mL measuring bottles, adds absolute ethyl alcohol to dissolve and is diluted to quarter
Degree, shakes up, produces.
Determination method precision measures reference substance solution and each 50 μ l of need testing solution, puts respectively in tool plug test tube, with anhydrous second
Alcohol is blank, is evaporated respectively；It is each to add 5% vanillic aldehyde-glacial acetic acid solution 0.2ml, perchloric acid 0.8ml, it is closed, shake up rearmounted
20 minutes in 70 DEG C of water-baths, taking-up is cooled to room temperature, adds ethyl acetate 5ml, after shaking up, according to AAS (《Chinese Pharmacopoeia》
The one annex V B of version in 2000), determine trap respectively at wavelength 560nm, calculate.
Determination Method of Flavone Content
The preparation of reference substance solution：Precision weighs control substance of Rutin 10.0mg, puts in 50mL measuring bottles, adds absolute ethyl alcohol to dissolve
And scale is diluted to, shake up, produce.
The preparation of standard curve：Reference substance 10mg is taken, solution of every 1mL containing 0.2mg is configured to absolute ethyl alcohol, takes successively
0.1mL, 0.2mL, 0.4mL, 0.8mL, 1.6mL are placed in 10mL volumetric flasks, and precision adds 1%AlCl3Solution 3mL, use are anhydrous
Ethanol constant volume, shake up rear room temperature and place 30min, with line blank, trap is surveyed at wavelength 370nm, using content as abscissa, inhale
Receipts degree is that ordinate draws standard curve.
The preparation of need testing solution takes test sample 10.0mg, puts in 50mL measuring bottles, adds absolute ethyl alcohol to dissolve and is diluted to quarter
Degree, shakes up, produces.
Determination method precision measures need testing solution 0.3mL, puts in 10mL measuring bottles, and precision adds 1%AlCl3Solution 3mL, use
Absolute ethyl alcohol constant volume, shake up rear room temperature and place 30min, with line blank.According to AAS, determined respectively at wavelength 370nm
Trap, calculate.
The general ginsenoside, Ampelopsis grossedentata total flavone and the leaf of bamboo that another object of the present invention there is provided said extracted are total
The composition of flavones composition is favorably improved the application in the medicine, health food and food of anoxia endurance in preparation.
Because present invention firstly discloses the group being made up of general ginsenoside, Ampelopsis grossedentata total flavone and bamboo extractive
Compound has the function that to be favorably improved anoxia endurance, therefore, by this composition individually or with other active constituents or auxiliary material
Medicament is made in cooperation, as long as the medicament is used to be favorably improved anoxia endurance, belongs to protection scope of the present invention.This hair
Bright composition is respectively provided with the effect of resist oxygen lack when any formulation is made.)
Composition (abbreviation composition, the example being made up of general ginsenoside, Ampelopsis grossedentata total flavone and bamboo extractive
4 are prepared) there is resisting oxygen lack, it is confirmed by following pharmacodynamic experiment.
The various raw materials that the present invention needs provide by Jilin Modern Chinese Medicine Engineering Research Center Co., Ltd..Nitrous acid
Sodium analyzes pure, soda lime, and lactic acid assay kit builds up Bioengineering Research Institute by Nanjing and produced, lot number 20130802.Sun
Property reference substance medicine rhodiola root ginseng Qi capsule (lot number：20131201, Sichuan Mai Liruo developments in science and technology Co., Ltd).
ICR mouse 160,18~22g of body weight, the high-new Experimental Animal Center in Changchun provide, experimental animal production licence
Number it is 2013-0010.
Each experiment takes mouse 80, is divided into 8 groups, every group 10.If 6 test groups, 1 blank control group and 1 sun
Property control group.The dosage of present composition test group is respectively 50mg/kg, 100mg/kg, 200mg/kg；General ginsenoside,
The dosage of Ampelopsis grossedentata total flavone and bamboo extractive is 200mg/kg；Blank control group gives distilled water；Positive control
Group dosage is 300mg/kg.Each group gavage is tested to sample, 1 time a day, continuous 30d, gavage volume is 0.1ml/10g body weight.
1st, influence of this composition to the mouse oxygen deficit tolerance time-to-live
The 1h after last gives sample, each group mouse is respectively put into the 250ml port grinding bottles for fill 5g soda limes (every bottle
1), seal, stopped with breathing as dead indication, observe the mouse survival time.Experimental result is shown in Table 1.
Influence of 1 composition of table to the mouse oxygen deficit tolerance time-to-live
Compared with blank control group, * P ＜ 0.05, * * P ＜ 0.01
As shown in Table 1, basic, normal, high three dosage group of the present composition can extend the time-to-live of mouse, and normal pressure is resistance to
The survival time under hypoxic condition is compared with blank control group, and high, middle dose group has significant difference.And during high dose group mouse survival
Between be longer than low dosage and middle dose group mouse.Experimental data shows simultaneously, and the present composition is high, the more single ginseng of middle dose group
Total saposins, Ampelopsis grossedentata total flavone and bamboo-leaves flavones have stronger pharmacological activity.
2nd, influence of the present composition to the mouse natrium nitrosum poisoning time-to-live
The 1h after last gives sample, each group animal are dynamic by 240mg/kg body weight doses intraperitoneal injection natrium nitrosum, observation
The thing time-to-live.Experimental result is shown in Table 2.
Influence of the present composition of table 2 to the mouse natrium nitrosum poisoning time-to-live
Compared with blank control group, * P ＜ 0.05, * * P ＜ 0.01
As shown in Table 2, compared with blank control group, 3 dosage groups of the present composition can be obviously prolonged mouse nitrous
Sour sodium is poisoned the time-to-live, has significant difference.Present composition height, the more single general ginsenoside of middle dose group, aobvious tooth
Ampelosis general flavone and bamboo extractive have stronger activity.
3rd, influence of the present composition to Acute cerebral ischemia in mice the survival time under hypoxic condition
The 1h after last gives sample, each group animal, by only breaking end, are recorded after mouse breaks end extremely by stopwatch immediately from neck
Dehisce dwell time of breathing.Experimental result is shown in Table 3.
Influence of the present composition of table 3 to Acute cerebral ischemia in mice the survival time under hypoxic condition
Compared with blank control group, * P ＜ 0.05, * * P ＜ 0.01
As shown in Table 3, present composition height, middle dose group can be obviously prolonged the survival of Acute cerebral ischemia in mice anoxic
Time.Present composition height, the more single general ginsenoside of middle dose group, Ampelopsis grossedentata total flavone and bamboo extractive have
Stronger activity.
4th, influence of the present composition to blood lactase acid after mouse movement
Take a blood sample, then do not born a heavy burden after temperature is 30 DEG C of water went swimming 10min after mouse last gives sample 30min
Taking-up is taken a blood sample again immediately, finally the 3rd blood sampling of rest 20min.By the Serum lactic acid content at 3 time points of kit measurement, and
Lactic acid TG-AUC is calculated as follows.Experimental result is shown in Table 4.
Blood lactase acid TG-AUC=5 × (before swimming after blood lactase acid value+3 × swimming 0min blood lactase acid value+2 × swimming
Rest 20min blood lactase acid value afterwards)
Influence of the present composition of table 4 to blood lactase acid after mouse movement
Compared with blank control group, * P ＜ 0.05, * * P ＜ 0.01
As shown in Table 4, present composition height, middle dose group can obviously reduce blood lactase acid TG-AUC after mouse movement
Content.So as to improve the energy supply of body glycometabolism system level, the ratio of protein metabolism energy supply is reduced, so as to improve anti anoxia
Ability.Meanwhile test data shows the more single general ginsenoside of the present composition, Ampelopsis grossedentata total flavone and leaf of bamboo extraction
Thing has stronger activity.
Embodiment
Embodiment 1 (granule)
1st, ginseng 10kg is taken, is extracted 2 times, 2 hours every time, filtered, filtrate recycling ethanol with 10 times of 60% alcohol refluxs of amount,
Ethanol extract is obtained, every 1ml medicinal materials containing 0.8g is added water to, stirs evenly, by the D-101 macroporous resin columns anticipated, first use
Deionized water elutes 5 times of column volumes, discards eluent, after with 70% ethanol elution, 4 times of column volumes, collects eluent, reclaims second
Alcohol, dry, obtain general ginsenoside.
With ginsenoside Rb1For reference substance, using determined by ultraviolet spectrophotometry, the content of total saposins is 83.9%.
2nd, ampelopsis grossdentata 20kg is taken, is extracted 2 times, 3 hours every time, filtered, filtrate recycling ethanol with 70% alcohol reflux,
Ethanol extract is obtained, every 1ml medicinal materials containing 1.0g is added water to, stirs evenly, by the AB-8 macroporous resin columns anticipated, first spend
3 times of column volumes of ion water elution, discard eluent, then with 20% ethanol elution, 3 times of column volumes, discard eluent, after with 80%
5 times of column volumes of ethanol elution, eluent is collected, reclaim ethanol, dried, obtain Ampelopsis grossedentata total flavone；Using rutin as reference substance,
Using determined by ultraviolet spectrophotometry, the content of general flavone is 85.7%.
3rd, leaf of bamboo 20kg, the leaf of bamboo are extracted 2-3 times with 5-9 times of water, each 1-2 hours, filtering, filtrate concentration, are dried, are obtained
Bamboo extractive；
4th, the general ginsenoside 50g that step 1 obtains is weighed, step 2 obtains Ampelopsis grossedentata total flavone 400g, and step 3 obtains
To bamboo leave flavone 150g, appropriate medical starch, it is well mixed, pelletizes, dry, whole grain, loads capsule, be made 1000, i.e.,
.
Embodiment 2 (tablet)
General ginsenoside, Ampelopsis grossedentata total flavone, the extracting method of bamboo leave flavone are the same as embodiment 1.
Weigh general ginsenoside 100g, Ampelopsis grossedentata total flavone 100g, bamboo leave flavone 450g, appropriate medical starch,
It is well mixed, pelletize, dry, whole grain, tabletting, be made 1000, produce.Orally, twice daily, 1 to 2 tablets once.
Embodiment 3 (soft sweets formulation)
1st, the extracting method of general ginsenoside, Ampelopsis grossedentata total flavone, bamboo leave flavone is the same as embodiment 1.
2nd, by glucose 2000g, white granulated sugar 150g, citric acid 80g, sodium citrate 8g, converted starch 80g, it is added to 1 times
Measure in water, heating makes it all dissolve, and crosses 100 mesh sieves, standby；By gelatin 800g, it is added in 8 times of amount water, heating makes its complete
Portion dissolves, and is mixed with the solution after above-mentioned sieving, adds general ginsenoside 8g, Ampelopsis grossedentata total flavone 20g, bamboo extractive
30g, heating boil, and let cool, with 1:1 converted starch and the mixture of peanut oil make releasing agent, the coating release agent on grinding tool, pour
Note, dry 1 hour, be stripped at 40 DEG C, packing, produce 1000 soft sweets.Chewed in oral cavity, 3 times a day, each 1-2 blocks.
Claims (3)
- A kind of 1. pharmaceutical composition with resisting oxygen lack, it is characterised in that the active component of the pharmaceutical composition and respectively into Point content ratio be：General ginsenoside 5-20 parts by weight, Ampelopsis grossedentata total flavone 10-40 parts by weight, bamboo extractive 15- 45 parts by weight.
- A kind of 2. pharmaceutical composition with resisting oxygen lack according to claim 1, it is characterised in that the pharmaceutical composition Active component and the content ratio of each composition be：The parts by weight of general ginsenoside 8, the parts by weight of Ampelopsis grossedentata total flavone 20, bamboo The parts by weight of leaf extract 30.
- 3. a kind of a kind of preparation method of the pharmaceutical composition with resisting oxygen lack as claimed in claim 1 or 2, its step It is：(1) take ginseng crude drug to be extracted 2-3 times with 50-95% alcohol refluxs, each 2-3 hours, filtering, filtrate recycling ethanol, obtain Ethanol extract, ethanol extract are first washed with deionized water de- by the D-101 large pore resin absorption columns anticipated, abandoned Eluent is removed, after with 50-90% ethanol elutions, eluent is collected, reclaims ethanol, dry, obtain general ginsenoside；(2) ampelopsis grossdentata medicinal material 50-90% alcohol refluxs are taken to extract 2-3 times, each 2-3 hours, filtering, filtrate recovery second Alcohol, obtains ethanol extract, and ethanol extract is first washed with deionized water by the AB-8 large pore resin absorption columns anticipated It is de-, discard eluent, then with 10-25% ethanol elutions, discard eluent, after with 60-90% ethanol elutions, collect eluent, Ethanol is reclaimed, dries, obtains Ampelopsis grossedentata total flavone；(3) take the water of 5-9 times of leaf of bamboo medicinal material to extract 2-3 times, each 1-2 hours, filtering, filtrate concentration, dry, obtain the leaf of bamboo and carry Take thing；(4) the general ginsenoside 5-20 parts by weight obtained by step (1) are taken, take Ampelopsis grossedentata total flavone 10- obtained by step (2) 40 parts by weight, bamboo extractive 15-45 parts by weight obtained by step (3) are taken, be well mixed, obtain with the natural of resisting oxygen lack Pharmaceutical composition；Appropriate amount of auxiliary materials is added, granule, tablet, soft sweets or other traditional oral preparations are made.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102210670A (en) * | 2011-04-08 | 2011-10-12 | 中国人民解放军总后勤部卫生部药品仪器检验所 | Use of flavonol compound in preparation of antihypoxic medicines or foods |
| CN103750143A (en) * | 2014-01-21 | 2014-04-30 | 中国人民解放军第三军医大学 | Anti-hypoxia and anti-fatigue energy composition and application thereof |
-
2015
- 2015-03-06 CN CN201510099231.7A patent/CN104940666B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102210670A (en) * | 2011-04-08 | 2011-10-12 | 中国人民解放军总后勤部卫生部药品仪器检验所 | Use of flavonol compound in preparation of antihypoxic medicines or foods |
| CN103750143A (en) * | 2014-01-21 | 2014-04-30 | 中国人民解放军第三军医大学 | Anti-hypoxia and anti-fatigue energy composition and application thereof |
Non-Patent Citations (2)
| Title |
|---|
| 人参总皂苷的耐缺氧效应;王涛等;《中国营养学会特殊营养第五届学术会议论文摘要汇编》;20020901;第77页倒数第2-3段 * |
| 竹叶提取物的抗缺氧作用;付晓春等;《中药新药与临床药理》;20050430;第16卷(第2期);第100页"摘要"，第102页右栏第1-2段 * |
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