CN104940307A - Traditional Chinese medicine composition for preventing and treating honeybee microsporidiosis and use thereof - Google Patents
Traditional Chinese medicine composition for preventing and treating honeybee microsporidiosis and use thereof Download PDFInfo
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- CN104940307A CN104940307A CN201510302650.6A CN201510302650A CN104940307A CN 104940307 A CN104940307 A CN 104940307A CN 201510302650 A CN201510302650 A CN 201510302650A CN 104940307 A CN104940307 A CN 104940307A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
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Abstract
The invention provides a traditional Chinese medicine composition for preventing and treating honeybee microsporidiosis. The traditional Chinese medicine composition comprises polygonum cuspidatum and szechwan chinaberry fruit aqueous extract products according to a volume ratio of 1: 1. The traditional Chinese medicine composition and sugar syrup with a concentration of 60-70% by mass are processed to form medicine-containing syrup with a concentration of 4-13% according to a certain volume ratio, and the medicine-containing syrup is freely eaten by honeybees suffered from honeybee microsporidiosis. Through action on honeybees and microsporidia, the traditional Chinese medicine composition can prevent and treat honeybee microsporidiosis. The traditional Chinese medicine composition reduces toxicity of szechwan chinaberry fruit in a single agent, has parasite expelling effects of szechwan chinaberry fruit and anti-inflammation and detumescence effects of polygonum cuspidatum and has basically no toxicity. The traditional Chinese medicine composition utilizes easily available medical raw materials with a wide source, can be prepared simply and has a low cost. The traditional Chinese medicine composition does not produce drug residue on honeybee products and the environment and does not pollute human and animals and the environment. The traditional Chinese medicine composition can be used in drugs for prevention and treatment on honeybee microsporidiosis.
Description
Technical field
The invention belongs to extracting method and the technical field of traditional Chinese medicine preparation of Chinese medicine.Be specifically related to a kind ofly prevent and treat Chinese medicine composition of Nosema apis parasitosis and its production and use.
Background technology
Nosema apis parasitosis, also known as " nosema disease ", is the common infectious disease of the digestive tract of a kind of adult honeybee that caused by Nosema apis worm.Nosema apis worm, mainly by infecting adult bees intestinal epithelial cell, destroys the normal stool function of Apis intestinal, reduces the immunocompetence of Apis, changes the collection behavior of Apis, shortens the life-span of Apis.Bee colony ill slight time, symptom is not obvious, usually out in the cold.And once serious, the collapse of whole bee colony even may be caused.The microsporidian of Apis mainly contain (west) Nosema apis worm (
nosema apis) and Eastern bee microsporidian (
nosema ceranae).In recent years large quantity research find Eastern bee microsporidian within the scope of world's bee-keeping a lot of area replace (west) Nosema apis worm gradually, become one of important disease endangering global apiculture.We also show the investigation of Apis mellifera field surely, Zhejiang Province, and the Nosema apis parasitosis caused by Eastern bee microsporidian is widely current, and some areas are in a bad way.
Amebacilin is the medicine uniquely effectively can preventing and treating Nosema apis parasitosis at present.As a kind of antibiotic, the life-time service of Amebacilin in bee colony can cause the drug residue problem of bee product.Existing large quantity research shows that Amebacilin can produce certain side effect to health in disease such as treatment mankind's encephalitis microsporidiosis and acquired immune deficiency syndrome (AIDS) etc.Therefore Amebacilin only allows registration to use in the minority bee-keeping country such as the U.S., Canada at present, and European Union member countries then prohibit the use this medicine.The measure of current China bee-keeping beekeeper in control Nosema apis parasitosis is mainly fed sour feedstuff, but poor effect.Along with growth in the living standard, people are also more and more higher to healthy, safety, free of contamination bee product pursuit.Therefore develop effectively preventing and treating Nosema apis parasitosis, seem very necessary to people and animals and comparatively safe, the unlikely residual natural plant extracts of environment.
Polygoni cuspidati,radix, property is flat, micro-hardship, nontoxic.Return liver, gallbladder, lung meridian.Rhizoma Polygoni Cuspidati has the effects such as blood circulation promoting and blood stasis dispelling, removing toxic substances and promoting subsidence of swelling, antiinflammatory, antibacterial and antiviral.Rhizoma Polygoni Cuspidati water decoction for oral administration, has the effect of hemostasis to intestinal bleeding.Its main component is emodin, chrysophanol, polidatin, aminoacid and the chemical composition such as polysaccharide and condensed tannin.
Fructus Toosendan is clinical common medicine." the contained Fructus Toosendan of Chinese Pharmacopoeia 2005 version is the dry mature fruit of Meliaceae plant Fructus Toosendan, and nature and flavor bitter cold is slightly poisonous.Return liver, small intestinal, urinary bladder channel, have soothing the liverly to expel the heat-evil, the effect such as promoting the circulation of QI to relieve pain, parasite killing.Be mainly used in the breast side of body, colic, abdominal pain due to worm stagnation etc. clinically, be also used for various diseases associated with inflammation with other medicines compatibility.
Summary of the invention
The object of this invention is to provide a kind of Chinese medicine composition preventing and treating Nosema apis parasitosis, be made up of the water extract equal-volume of Rhizoma Polygoni Cuspidati and Fructus Toosendan.
Pharmaceutical composition of the present invention is prepared by following steps:
(1) Rhizoma Polygoni Cuspidati extracting solution preparation: after the Rhizoma Polygoni Cuspidati getting 10 g soaks 2 h in the cold water of 100 ml, adopts decocting method to be heated to boil after 10-20 min, gets filtrate, then add the water of 100 ml in residue, reheating to boiling, getting filtrate.Merge twice filtrate.Cryopreservation filtrate.
(2) Fructus Toosendan extracting solution preparation: after the Fructus Toosendan of 10 g soaks 2 h in the cold water of 100 ml, adopts decocting method to be heated to boil after 10-20 min, gets filtrate.In residue, add the water of 100 ml again, reheating to boiling, getting filtrate.Merge twice filtrate.Cryopreservation filtrate.
(3) by Rhizoma Polygoni Cuspidati extracting solution and the mixing of Fructus Toosendan extracting solution equal-volume, to obtain final product.
Another object of the present invention is to provide the application of described pharmaceutical composition in the medicine of preparation control Nosema apis parasitosis.Realize especially by following steps: the sucrose syrup being 60-70% by compatibe drug compositions and mass percent concentration is mixed with the medicated syrup that concentration is 4%-13% by volume, allow the Apis infecting microsporidian freely take food.Act on Apis and microsporidian, thus reach the effect of control Nosema apis parasitosis.
The present invention has following characteristics: (1) reduces the toxicity of single dose Chinese medicine Fructus Toosendan, and given full play to effect of Fructus Toosendan anthelminthic effect and Rhizoma Polygoni Cuspidati anti-inflammation detumescence, toxicity is zero substantially; (2) medicine wide material sources, be easy to get, produce simple, cost is low; (3) there is not the drug residue problem to bee product and environment, to people and animals and environment " no pollution ".
Detailed description of the invention
The present invention is further described in conjunction with the embodiments.
embodiment 1
Take the Rhizoma Polygoni Cuspidati of 10 g after drying at room temperature and Fructus Toosendan respectively, add the cold water of 100 ml respectively, by two kinds of complete submergences of Chinese crude drug material, after left at room temperature 2 h, after first intense fire boils the micro-10-20 min that boils of slow fire again, cross leaching filtrate.In the residue after filtration, add the cold water of 100 ml again, repeat above fried step, get filtrate, merge twice filtrate, low temperature seal is preserved.The sucrose syrup being 60% with mass percent concentration after Rhizoma Polygoni Cuspidati water extract and Fructus Toosendan water extract equal-volume compatibility is mixed with the medicated syrup that concentration is 4% by volume, allows the Apis infecting microsporidian freely take food.
Result: the 7th d and the 13rd d after infection, the microsporidian spore number in the infected Apis body of medicated syrup of feeding be respectively feed not medicated syrup infected Apis 50% and 80%.13rd d after infection, the infected bee survival of dispenser group is 93%, and the infected bee survival of medicine feed group is not 50%.
embodiment 2
Take the Rhizoma Polygoni Cuspidati of 10 g after drying at room temperature and Fructus Toosendan respectively, add the cold water of 100 ml respectively, by two kinds of complete submergences of Chinese crude drug material, after left at room temperature 2 h, after first intense fire boils the micro-10-20 min that boils of slow fire again, cross leaching filtrate.In the residue after filtration, add the cold water of 100 ml again, repeat above fried step, get filtrate, merge twice filtrate, low temperature seal is preserved.The sucrose syrup being 60% with mass percent concentration after Rhizoma Polygoni Cuspidati water extract and Fructus Toosendan water extract equal-volume compatibility is mixed with the medicated syrup that concentration is 7% by volume, allows the Apis infecting microsporidian freely take food.
Result: the 7th d and the 13rd d after infection, the microsporidian spore number of feeding in the infected Apis body of medicated syrup be respectively feed not medicated syrup infected Apis 33.5% and 35.4%, 13rd d after infection, the infected bee survival of dispenser group is 95%, and the infected bee survival of medicine feed group is not 50%.
embodiment 3
Take the Rhizoma Polygoni Cuspidati of 10 g after drying at room temperature and Fructus Toosendan respectively, add the cold water of 100 ml respectively, by two kinds of complete submergences of Chinese crude drug material, after left at room temperature 2 h, after first intense fire boils the micro-40-60 min that boils of slow fire again, cross leaching filtrate.In the residue after filtration, add the cold water of 100 ml again, repeat above fried step, get filtrate, merge twice filtrate, low temperature seal is preserved.The sucrose syrup being 60% with mass percent concentration after Rhizoma Polygoni Cuspidati water extract and Fructus Toosendan water extract equal-volume compatibility is mixed with the medicated syrup that concentration is 13% by volume, allows the Apis infecting microsporidian freely take food.
Result: the 7th d and the 13rd d after infection, the microsporidian spore number in the infected Apis body of medicated syrup of feeding be respectively feed not medicated syrup infected Apis 40% and 50%.13rd d after infection, the infected bee survival of dispenser group is 70%, and the infected bee survival of medicine feed group is not 50%.
embodiment 4
Take the Rhizoma Polygoni Cuspidati of 10 g after drying at room temperature and Fructus Toosendan respectively, add the cold water of 100 ml respectively, by two kinds of complete submergences of Chinese crude drug material, after left at room temperature 2 h, after first intense fire boils the micro-10-20 min that boils of slow fire again, cross leaching filtrate.In the residue after filtration, add the cold water of 100 ml again, repeat above fried step, get filtrate, merge twice filtrate, low temperature seal is preserved.The sucrose syrup being 70% with mass percent concentration after Rhizoma Polygoni Cuspidati water extract and Fructus Toosendan water extract equal-volume compatibility is mixed with the medicated syrup that concentration is 7% by volume, allows the Apis infecting microsporidian freely take food.
Result: the 7th d and the 13rd d after infection, the microsporidian spore number of feeding in the infected Apis body of medicated syrup be respectively feed not medicated syrup infected Apis 35% and 40%, 13rd d after infection, the infected bee survival of dispenser group is 95%, and the infected bee survival of medicine feed group is not 50%.
Claims (4)
1. prevent and treat a Chinese medicine composition for Nosema apis parasitosis, it is characterized in that, be made up of the water extract equal-volume of Rhizoma Polygoni Cuspidati and Fructus Toosendan.
2. a kind of Chinese medicine composition preventing and treating Nosema apis parasitosis according to claim 1, be is characterized in that, prepared by following steps:
(1) Rhizoma Polygoni Cuspidati extracting solution preparation: the Rhizoma Polygoni Cuspidati getting 10 g soaked after 2 hours in the cold water of 100 ml, after employing decocting method is heated to boil 10-20 minute, gets filtrate, in residue, add the water of 100 ml again, reheating to boiling, getting filtrate, merge twice filtrate, cryopreservation filtrate;
(2) Fructus Toosendan extracting solution preparation: the Fructus Toosendan of 10 g soaked after 2 hours in the cold water of 100 ml, after employing decocting method is heated to boil 10-20 minute, gets filtrate, in residue, add the water of 100 ml again, reheating to boiling, getting filtrate, merge twice filtrate, cryopreservation filtrate;
(3) by Rhizoma Polygoni Cuspidati extracting solution and the mixing of Fructus Toosendan extracting solution equal-volume, to obtain final product.
3. the application of pharmaceutical composition according to claim 1 in the medicine of preparation control Nosema apis parasitosis.
4. application according to claim 3, it is characterized in that, realized by following steps: the sucrose syrup being 60-70% by described pharmaceutical composition and mass percent concentration is mixed with the medicated syrup that concentration is 4%-13% by volume, allow the Apis infecting microsporidian freely take food.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105998147A (en) * | 2016-05-27 | 2016-10-12 | 骆开友 | Composition for preventing and treating Malpighian tube amoebiasis of bees |
| CN106176886A (en) * | 2016-07-13 | 2016-12-07 | 熊明雷 | A kind of compositions for preventing and treating amebiasis of bee |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101156940A (en) * | 2007-09-12 | 2008-04-09 | 严必福 | Sporozoan cleaning fish medicine |
| CN101357194A (en) * | 2008-09-11 | 2009-02-04 | 吴国良 | Traditional Chinese medicine for treating Pafeng disease |
| CN103656020A (en) * | 2012-09-24 | 2014-03-26 | 天津瑞贝特科技发展有限公司 | Traditional Chinese medicine composition for treating encephalitozoon intestinalis of freshwater fish and preparation method thereof |
-
2015
- 2015-06-05 CN CN201510302650.6A patent/CN104940307B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101156940A (en) * | 2007-09-12 | 2008-04-09 | 严必福 | Sporozoan cleaning fish medicine |
| CN101357194A (en) * | 2008-09-11 | 2009-02-04 | 吴国良 | Traditional Chinese medicine for treating Pafeng disease |
| CN103656020A (en) * | 2012-09-24 | 2014-03-26 | 天津瑞贝特科技发展有限公司 | Traditional Chinese medicine composition for treating encephalitozoon intestinalis of freshwater fish and preparation method thereof |
Non-Patent Citations (2)
| Title |
|---|
| 胡元亮: "《中兽医学》", 31 December 2006 * |
| 陈世壁等: "《程序化养蜂法》", 30 June 2010 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105998147A (en) * | 2016-05-27 | 2016-10-12 | 骆开友 | Composition for preventing and treating Malpighian tube amoebiasis of bees |
| CN106176886A (en) * | 2016-07-13 | 2016-12-07 | 熊明雷 | A kind of compositions for preventing and treating amebiasis of bee |
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| CN104940307B (en) | 2018-04-17 |
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