CN104920868B - Epigallo-catechin gallate (EGCG)（EGCG）Application in terms of pig feed additive is prepared - Google Patents
Epigallo-catechin gallate (EGCG)（EGCG）Application in terms of pig feed additive is prepared Download PDFInfo
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- CN104920868B CN104920868B CN201510314609.0A CN201510314609A CN104920868B CN 104920868 B CN104920868 B CN 104920868B CN 201510314609 A CN201510314609 A CN 201510314609A CN 104920868 B CN104920868 B CN 104920868B
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Abstract
The invention discloses Epigallo-catechin gallate (EGCG)（EGCG）As or prepare the pannage of the application and preparation in pig feed additive.Specially EGCG is applied to prepare the feed addictive for reducing Animal muscles I types Muscle fiber density or reducing the ratio of red meat in Animal muscles.Present invention discover that EGCG is added in pannage can reduce skeletal muscle I fiber type ratios, the ratio of red meat in Animal muscles is reduced, improves the health of meat.And, the pannage that with the addition of EGCG is a kind of safe feed, it is not contained to swinery growth and the poisonous and hazardous material of swinery health, EGCG additive amounts are less, it can be directly added into premix, adding procedure is convenient and efficient, and preparation method is simple, there is no especial equipment requirements, there is good popularizing application prospect.
Description
Technical field
The invention belongs to field of feed additive technology.More particularly, to Epigallo-catechin gallate (EGCG)
（EGCG）Application in terms of pig feed additive is prepared.
Background technology
Feed addictive is for some special requirements, and what is artificially added in various cooperations or mixed fodder has not
With the material of purposes, it is the nutrition improved with fortified feed that it, which is acted on, promotes pig growth and development, prevention disease, improves feed
Utilization rate and the quality of meat product etc..With animal nutrition, physiology, thremmatology, biochemistry, biotechnology, pharmacology
The development of the subjects such as, microbiology, present feed addictive fused each door subject and multiple technologies, its function,
Species and application have obtained huge expansion.
On the other hand, with the improvement of living standards, consumer has pork health the requirement of higher, feed is passed through
Additive approach, can change the type of meat fiber, reduce the ratio of red meat, so as to improve the health of meat.Therefore, develop
Study that new feed addictive is extremely important, animal is effectively improved while the quality safety of livestock products is ensured to ensure to realize
The health of meat.
Epigallo-catechin gallate (EGCG)（Epigallocatechin gallate, EGCG）It is most to have in tea polyphenols
The active ingredient of effect, belongs to catechin.EGCG has antibacterial, antiviral, anti-oxidant, anti arteriosclerosis, antithrombus formation, anti-blood
Pipe hyperplasia, anti-inflammatory and the effect such as antitumor.The correlative study that Animal muscles fiber type influences still is belonged in relation to EGCG at present
Blank, no correlation technique report.
The content of the invention
The technical problem to be solved in the present invention is the pig feed additive for overcoming the shortcomings of existing improvement pork health, carry
For applications of the EGCG in terms of the feed addictive for changing Animal muscles fiber type is prepared.Present invention discover that EGCG is added into pig
In feed, the ratio of Animal muscles I type muscle fibres can be reduced, reduces the feed addictive of the content of red meat, is one kind of EGCG
New application, has widened the application range of EGCG.
The object of the present invention is to provide Epigallo-catechin gallate (EGCG)（EGCG）Preparing pig feed additive side
The application in face.
It is a further object to provide a kind of new pannage for including EGCG.
It is a still further object of the present invention to provide the preparation method of the new pannage comprising EGCG.
Above-mentioned purpose of the present invention is achieved through the following technical solutions：
Epigallo-catechin gallate (EGCG)（EGCG）As pig feed additive or preparing pig feed additive
The application of aspect.
Specifically applied to the feed addictive for preparing change Animal muscles fiber type.
More specifically, being the feed addictive for being applied to prepare the ratio for reducing Animal muscles I type muscle fibres, and apply
In the feed addictive for preparing the ratio of red meat in reduction Animal muscles.
The present invention has found by a large amount of Animal experiments and molecular biology test, is added in the basal diet of pig
EGCG, can significantly reduce the ratio of Animal muscles I type muscle fibres, reduce the content of red meat.
In addition, EGCG can be applied to prepare the preparation that can reduce MyHCI protein expressions, can be reduced applied to preparing
The preparation of the expression of MyHC I mRNA, applied to prepare can reduce mitochondria synthesis related gene PGC-1 α, mtTFA and
The preparation of the protein expression of NRF-1.
A kind of new pannage, including EGCG and conventional feedstuff.Preferably, the EGCG containing in pannage
Amount is：0.1~2kg/ tons of pannages.
It is highly preferred that contents of the EGCG in pannage is：0.1~0.5kg/ tons of pannages.
Most preferably, contents of the EGCG in pannage is：0.5kg/ tons of pannages.
As one kind can embodiment, the pannage includes EGCG, feed premix raw material and complete feed raw material；Or
Include EGCG, absorption carrier and complete feed raw material.
Wherein, the feed premix raw material is preferably vitamin or mineral substance premix with reference to routine；The full price is raised
It is preferably corn, dregs of beans, fish meal or other unconventional cake rations with reference to routine to expect raw material；The absorption carrier can be ability
The common carrier in domain, is preferably wheat bran, corn flour or mountain flour etc..
As it is a kind of preferably can embodiment, the pannage is by EGCG that mass ratio is 0.01~0.05%, 4% feeding
Material premix raw material and 95.95~95.99% complete feed raw material composition, or by mass ratio be 0.01~0.05% EGCG,
4% absorption carrier and 95.95~95.99% complete feed raw material composition.
The preparation method of above-mentioned pannage includes the following steps：
S1. according to mass ratio, EGCG and feed premix raw material are carried out being mixed to get premix, or EGCG and absorption are carried
Body carries out being mixed to get pre-mixing agent；
S2. the step S1 premixes being mixed to get or pre-mixing agent are mixed with complete feed raw material, obtains the pig
Feed.
Wherein, step S1 can use conventional small-sized mixing apparatus to be mixed；Described in step S2；Premix or pre-mixing agent
With complete feed raw material mix and can use conventional large-scale co-grinding equipment.
The invention has the advantages that：
（1）The present invention provides a kind of important new opplication of EGCG.The first public EGCG of the present invention can be significantly reduced
The ratio of Animal muscles I type muscle fibres, reduces the content of red meat, can be used as or prepare a kind of pig feed additive, to improving pig
Meat health is of great significance.
（2）The present invention provides a kind of new pannage, the pannage that with the addition of EGCG is a kind of safe feed, it is not
Containing to swinery growth and the poisonous and hazardous material of swinery health.Compared with general feed addictive, it can not only be reduced
The ratio of Animal muscles I type muscle fibres, reduces the content of red meat, improves the health of pork, and EGCG is from Chinese green tea
A kind of composition of middle extraction, it is the main activity and water-soluble components of green tea, is the highest component of content in catechin.EGCG
With very strong antioxidation activity, antioxidation activity is at least ascorbic more than 100 times, is 25 times of vitamin E, can
Protect cell and DNA undermined, adverse effect will not be produced to people and surrounding environment.
（3）For EGCG disclosed by the invention as main feed additive, additive amount is less, can be directly added into premix,
Adding procedure is convenient and efficient, and preparation method is simple, without especial equipment requirements, has good popularizing application prospect.
Brief description of the drawings
Fig. 1 is influences of the EGCG to pig longissimus dorsi muscle proportion of fibers.
Fig. 2 is the influence that EGCG expresses pig longissimus dorsi muscle myoglobulin heavy chain.
Fig. 3 is influences of the EGCG to pig longissimus dorsi muscle mitochondria biogenesis related gene expression.
Embodiment
The present invention is further illustrated below in conjunction with Figure of description and specific embodiment, but embodiment is not to the present invention
Limit in any form.Unless stated otherwise, the reagent of the invention used, method and apparatus routinely try for the art
Agent, method and apparatus.
Unless stated otherwise, following embodiments agents useful for same and material are purchased in market.
Embodiment 1 prepares the pannage for including EGCG
1st, prepare
（1）Conventional small-sized mixing apparatus can be used, EGCG and feed premix raw material are carried out being mixed to get premix, or
Carry out being mixed and made into pre-mixing agent with carrier.
The feed premix raw material is vitamin or mineral substance premix；The carrier is wheat bran or corn flour etc.；It is described
Feed premixes the dosage of raw material and carrier with reference to conventional.
（2）Using conventional large-scale co-grinding equipment, by step（1）The premix being mixed to get or pre-mixing agent and full price
Feedstuff is mixed, and complete feed is made.
The complete feed raw material is corn, dregs of beans, fish meal or the other unconventional cake ration crushed.
2nd, pannage manufactured in the present embodiment, each formula percentage composition are as shown in table 1：
Table 1
| Constituent content | Formula 1 | Formula 2 | Formula 3 |
| EGCG（%/ton） | 0.01 | 0.025 | 0.05 |
| Premix（%/ton） | 4 | 4 | 4 |
| Complete feed（%/ton） | 95.99 | 95.975 | 95.95 |
Influences of 2 EGCG of embodiment to fattening pig muscle fiber types
Carried out using the formula 2 of embodiment 1 and formula 3 as pannage and tested
1st, in order to study influences of the EGCG to Animal muscles fiber type, the miscellaneous fattening pig 180 of 70 kilograms or so ternarys is selected
Head, approaches, the ratio that male and female is fifty-fifty is randomly divided into 3 groups, every group of 6 repetitions according to weight, each to repeat 10.
Test group：The complete feeds for adding formula 2 and formula 3 respectively in basal diet are fed respectively.
Control group, feeds basal diet.
2nd, experimental result
（1）At the end of feeding experiment, pig 18 of the selection in average weight or so（It is each to repeat one）, butcher, adopt
Collect longissimus dorsi muscle.EGCG is detected to different muscle fiber typeses using ATP enzyme decoration method（I types, IIa types and IIb types）The shadow of ratio
Ring, as a result as shown in Figure 1.
From attached drawing 1, compared with control group, 0.025% EGCG groups and 0.05% EGCG groups can reduce to a certain extent
The ratio of I types muscle fibre parting in longissimus dorsi muscle.
（2）EGCG is detected to different type myoglobulin heavy chain using quantitative PCR method and western blot methods at the same time
（MyHCI and MyHCII）MRNA and protein expression influence, as a result as shown in Figure 2.
It was found from attached drawing 2A, 0.025% EGCG and 0.05% EGCG can significantly reduce the protein expression of MyHCI, and right
The protein expression of MyHCII does not make significant difference.Meanwhile 0.025%, 0.05% EGCG can also significantly reduce MyHC I mRNA's
Expression（Fig. 2 B）.
（3）Finally, the influence that EGCG expresses mitochondria synthesis related gene is also have detected, as a result as shown in Figure 3.
Mitochondria synthesis related gene PGC-1 can be significantly reduced by adding EGCG/ tons of 0.5Kg it was found from attached drawing 3, in daily ration
The expression of α, mtTFA and NRF-1 albumen；It is synthesis related to significantly reduce mitochondria by EGCG/ tons of 0.25Kg of addition in daily ration
Because of the expression of PGC-1 α and mtTFA albumen.
In conclusion result of the test shows, the additive of EGCG, addition comprising EGCG or cooperation are added in the daily ration of pig
Feeding includes the feed of EGCG, can reduce I type muscle fiber typeses by reducing the expression of Muscle Mitochondria synthetic gene
Ratio, reduces red meat content, improves meat health.
Claims (3)
1. Epigallo-catechin gallate (EGCG) EGCG is as pig feed additive or in terms of pig feed additive is prepared
Application, it is characterised in that applied to preparing the ratio for reducing Animal muscles I type muscle fibres and reduce red meat in Animal muscles
The feed addictive of ratio；The feed addictive, which refers to that Epigallo-catechin gallate (EGCG) EGCG is used to prepare, to drop
Low MyHCI protein expressions, reduce mitochondria synthesis related gene PGC-1 α, mtTFA and NRF-1 protein expression preparation.
2. application according to claim 1, it is characterised in that by EGCG of the mass ratio for 0.01~0.05%, 4% feed
Raw material and 95.95~95.99% complete feed raw material composition pannage are premixed, or by mass ratio is 0.01~0.05%
EGCG, 4% absorption carrier and 95.95~95.99% complete feed raw material composition pannage；The feed premix raw material is dimension
Raw element or mineral substance premix；The complete feed raw material is corn, dregs of beans, fish meal or other unconventional cake rations；The suction
Appendix body is wheat bran, corn flour or mountain flour.
3. application according to claim 1, it is characterised in that pannage is prepared by the following method：
S1. according to mass ratio, EGCG and feed premix raw material be mixed to get premix, or by EGCG and absorption carrier into
Row is mixed to get pre-mixing agent；
S2. the step S1 premixes being mixed to get or pre-mixing agent are mixed with complete feed raw material, obtains the pannage.
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| CN201510314609.0A CN104920868B (en) | 2015-06-10 | 2015-06-10 | Epigallo-catechin gallate (EGCG)（EGCG）Application in terms of pig feed additive is prepared |
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| CN108208479B (en) * | 2018-01-18 | 2021-05-28 | 安徽农业大学 | Pure artificial feed suitable for feeding and experimental analysis of tea geometrid larvae |
| CN114304442A (en) * | 2022-01-12 | 2022-04-12 | 安徽农业大学 | Protein-saving fish feed and preparation and application thereof |
| CN114431340A (en) * | 2022-01-26 | 2022-05-06 | 广州微特加生物工程有限公司 | Application of catechin in preparation of animal feed additive and feed thereof |
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| CN101167719A (en) * | 2007-11-02 | 2008-04-30 | 广东省实验动物监测所 | Gallo catechin and gallate microcapsule granule and preparation technique thereof |
| CN101370555A (en) * | 2005-06-07 | 2009-02-18 | 帝斯曼知识产权资产管理有限公司 | Novel use of (-)-epigallocatechin gallate |
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| CN101370555A (en) * | 2005-06-07 | 2009-02-18 | 帝斯曼知识产权资产管理有限公司 | Novel use of (-)-epigallocatechin gallate |
| CN101167719A (en) * | 2007-11-02 | 2008-04-30 | 广东省实验动物监测所 | Gallo catechin and gallate microcapsule granule and preparation technique thereof |
| CN101715878A (en) * | 2009-11-24 | 2010-06-02 | 长沙树人牧业科技有限公司 | Functional feed for preventing PSE pork and application thereof |
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