CN104906163B - Yellow bur reality and its hypoglycemic purposes of extract - Google Patents
Yellow bur reality and its hypoglycemic purposes of extract Download PDFInfo
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- CN104906163B CN104906163B CN201510323281.9A CN201510323281A CN104906163B CN 104906163 B CN104906163 B CN 104906163B CN 201510323281 A CN201510323281 A CN 201510323281A CN 104906163 B CN104906163 B CN 104906163B
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Abstract
The present invention provides the purposes that yellow bur is real or its extract is in preparing hypoglycemic drug, health products or food.Present invention research is found, yellow bur has good function of blood sugar reduction in fact, amount of insulin can be increased, its hypoglycemic effect is suitable with one line chemicals glibenclamide of diabetes, but different, yellow bur material object will not lead to hepar damnification, good security, the scope of application is wider, is more suitable for treating and preventing food, health products or the drug of diabetes.
Description
Technical field
The invention belongs to field of health care products, and in particular to the new medical use of yellow bur reality and its extract.
Technical background
According to statistics, the number of patients of China's diabetes has had reached 92,400,000 at present, occupies (Yang W, et first of the whole world
Al.N Engl J Med, 2010,362 (12)：1090-1101).Up to 173,400,000,000 yuan every year of diabetes mellitus in China medical expense, sugar
Urine disease caused by direct medical expenses accounted for Chinese medical total expenses 13% (Alcorn T, et al.Lancet,
2012,379 (9833)：2227-2228).It can be seen that diabetes not only seriously endanger the health of the people, but also it is national band
Carry out heavy financial burden.Thus, prevention diabetes are very urgent.
Huang thorn, the straight fringe barberry Berberis dasystachya Maxim. of Berberidaceae plant are (black with thorn, sea-buckthorn in vain
Thorn) equally it is the medicinal and edible traditional wild acinus of the ethnic groups such as illiteracy, Tibetan, Uygur, it is referred to as " thorn of Qinghai three ".《It is green
The common Chinese herbal medicine handbook in sea》In Cortex berberidis dasystachyae (i.e. root skin) is documented：Cortex berberidis dasystachyae is that dicotyledon medicine Berberidaceae is planted
The stem skin of the straight fringe barberry of object.Brief description has also been made in its fruit medicine function, has and treats abdominal pain, indigestion, abdominal distension,
The functions such as dysentery.
It yet there are no to yellow bur hypoglycemic report in fact.
Invention content
The purpose of the present invention is to provide yellow bur reality and its new applications of extract.
Specifically, the present invention provides yellow bur realities or its extract to prepare hypoglycemic drug, health products or food
In purposes.
Further, the drug, health products or food are to prevent or treat I types, type-2 diabetes mellitus or/and diabetes simultaneously
Send out drug, health products or the food of disease.
Further, the drug, health products or food are to prevent or treatment liver function is normal or the medicine of impaired subjects
Object, health products or food.
The present invention is equally suitable for liver function damage glycosuria the study found that yellow bur will not lead to liver function damage in fact
The treatment of patient.
Wherein, the drug, health products or food are drug, health products or the food for improving insulin level.
Further, the yellow thorn fruit extract is the water extract of yellow bur reality.According to yellow bur it is real and it is water-soluble at
Point --- polysaccharide all this results with hypoglycemic effect can estimate without doubt, and the water extract of yellow bur reality also has
Hypoglycemic effect.
Wherein, the yellow thorn is the straight fringe barberry Berberis dasystachya Maxim. of Berberidaceae plant.
Wherein, the drug, health products or food are peroral dosage form；Further, the peroral dosage form is selected from tablet, glue
Wafer, pill, granule, powder, extract or oral solution.
The present invention can increase amount of insulin the study found that yellow bur reality and its extract have good function of blood sugar reduction,
Its hypoglycemic effect and one line chemicals glibenclamide of diabetes are suitable but different, and yellow bur material object will not be led
Cause hepar damnification, good security, the scope of application is wider, be more suitable for treating and preventing the food of diabetes, health products or
Drug.
Specific implementation mode
The pharmacodynamic study of the yellow bur reality of 1 present invention of embodiment
1) experimental drug：It takes yellow bur real, directly crushes that obtain fruit powder spare after dry.Dosage based on rat body weight,
Gavage low dosage, high dose are respectively 0.4g/kg, 1.6g/kg (with yellow bur powder dosage).
2) animal：Four week old Kunming rats, male, regular grade, 135g are provided by Gansu Chinese of Traditional Chinese Medicine.In in laboratory
It is placed in raise in mouse cage and be tested after a week (per 8, cage, 22 ± 1 DEG C of temperature, humidity 42% ± 2%).It is divided into five groups：Point
Not Wei blank group (intravenous injection the non-modeling of citrate buffer solution, ultra-pure water gavage), (tail vein injection STZ modelings surpass model group
Pure water gavage), positive drug group (tail vein injection STZ modelings, glibenclamide 20mg/kg gavages), yellow bur powder low dose group (tail
It is injected intravenously STZ modelings, yellow bur powder gavage 0.4g/kg), yellow bur powder high dose group (tail vein injection STZ modelings, Huang thorn
Fruit powder gavage 1.6g/kg).Modeling method：Fasting 16h in advance then carries out it STZ (citrate buffer solution preparation) tail vein
60mg/kg is injected, after injection, surveys within 72 hours its blood glucose, testing model success or not.
3) experiment reagent：95% medicinal alcohol, Nanjing Ning Shi chemical reagent Co., Ltd；Physiological saline, Shandong all medicine together
Industry Co., Ltd；Cholesterol, triglyceride, high-density lipoprotein, low-density lipoprotein, glutamic-oxalacetic transaminease, glutamic-pyruvic transaminase, fat
Albumen esterase, liver esterase kit, bio tech ltd is built up in Nanjing.
4) laboratory apparatus：Bio-Rad680 type microplate reader, Bio Rad Laboratories；DS-1 type tissue Syrup-homogenizing instruments, Shanghai are just intelligent
Trade Co., Ltd.；TGL-16G-A type tube centrifuges, Anting Scientific Instrument Factory, Shanghai；The visible uv-spectrophotometrics of UV-722N
Meter, Shanghai Ni Ke Co., Ltds；HD type thermostat water baths, Constant Temp. Instrument Factory, Liaoyang.
5) experimentation：Healthy SD rat 40 is taken, 5 groups is randomly divided by weight, is divided into blank group, model group, the positive
Medicine group and low, high dose group.Blank group and the daily gavage of model group give distilled water；The daily gavage of positive drug group gives Ge Lieben
Urea 1 time；Yellow bur powder is given to the daily gavage of test medicine group 1 time, and six groups are continuously given 28d；Meanwhile it being measured every 7 days
Its insulin and blood glucose；After last gives tested material, after anesthesia, blood, acquired blood centrifuging and taking supernatant, for examining are taken through arteria carotis
Survey blood lipids index and glutamic-oxalacetic transaminease, glutamic-pyruvic transaminase.To its death, rat liver tissue is taken, table is washed away with physiological saline
Face blood stains, are blotted with filter paper, and tissue Syrup-homogenizing instrument is homogenized, and is collected in homogenate to centrifuge tube, and 10min is centrifuged with 3000rpm,
Aspirate supernatant carries out lipoproteinesterase and liver esterase determination of activity.
6) yellow bur powder reduces the measurement and result for suffering from the blood glucose in diabetes rat body
Influence (Mean ± SD) of the yellow bur powder of table 1 to the STZ rat blood sugars induced
Note：With normal group comparative groupaP ＜ 0.05；Compared at modulebP ＜ 0.05；
Influence (Mean ± SD) of the yellow bur powder of table 2 to the STZ rat insulins induced
Note：With normal group comparative groupaP ＜ 0.05；Compared at modulebP ＜ 0.05；
By table 1,2 it is found that yellow bur can effectively reduce STZ blood glucose in diabetic rats in fact, amount of insulin is increased, and act on
It is suitable with positive drug, show that yellow bur can be used for the treatment to diabetes in fact.
7) yellow bur powder reduces the measurement and result for suffering from the blood fat in diabetes rat body
The diabetes of STZ inductions, can make rat Islet cells that karyopycnosis and hyaloid lesion occur.Its lipid metaboli with
Get muddled.The lipid metaboli of rat gets muddled simultaneously, and related blood lipids index and the relevant enzymatic activity of lipid metaboli also occur disorderly
Disorderly.
Rat Cholesterol TC, triglycerides TG, the high-density lipoprotein HDL that the yellow bur powder of table 3 induces STZ, low-density
The influence (Mean ± SD) of lipoprotein LDL
Note：Compared with normal groupaP ＜ 0.05；Compared at modulebP ＜ 0.05
As shown in Table 3, the yellow bur powder of the present invention is low, high dose group is to the STZ diabetes rats induced cholesterol, glycerine
Three esters, low-density lipoprotein, which have, adjusts reduction effect, to high-density lipoprotein by adjusting effect of increasing.And it is closed in dosage effect
System, high dose group and the significant difference of model group (p ＜ 0.05).Meanwhile experiment shows the effect dative row of high dose group originally
The effect of urea is suitable, and glibenclamide has body certain side effect as chemically synthesized drug, therefore, it is suggested that adopting
It can be made of the i.e. yellow bur powder of natural plants and substitute health products use.
HL is that have phosphorus A1 and triglyceride hydrolytic enzyme activities by one kind that hepatic parenchymal cells synthesize, to plasma lipid transport
The extracellular protein to play an important role, be primarily involved in HDL-C reconstruct and chylomicron remains, low-density lipoprotein metabolism and
The antiport of cholesterol.HL vigor is abnormal to have higher correlation with atherosclerosis and diabetes.LPL is lipoprotein generation
The key enzyme thanked is key enzyme of the hydrolysis rich in triglyceride in three casein of glycerine.Its major function is hydrolysis CM and VLDL
In triglyceride be glycerine and aliphatic acid.Lipoproteinesterase LPL and liver esterase HL can decompose triglycerides TG and decompose
For glycerine and free fatty FFA, fat can be calculated separately out by measuring the free fatty that its decomposition generates using copper reagent
The activity of albumen esterase and liver esterase.
With ALT contents under the stimulation of some drugs, internal hepatic tissue receives adipocyte and invades profit AST, influences correlation
Hepatocyte enzyme activity, and under normal circumstances, as Simvastatin and glibenclamide etc. can all have a certain impact to hepatic tissue.
Rat lipoproteinesterase LPL that the yellow bur powder of table 4 induces STZ, liver esterase HL, glutamic-oxalacetic transaminease AST, paddy third
The influence (Mean ± SD) of transaminase ALT
Note：Compared with normal groupaP ＜ 0.05；Compared at modulebP ＜ 0.05；Compared with normal groupcP ＜ 0.05.
Yellow bur powder can be such that HL the and LPL activity of experimental rat increases, and LPL is to remove the speed limit rich in TG lipoprotein
Enzyme, to play its important function in lipoprotein cycle.The HL activity of diabetes rat can be made to increase simultaneously, to logical
HL is crossed as ligand, promotes liver cell intake cholesterol and chylomicron remnant, reduces total cholesterol and triglyceride in blood plasma
Concentration, and then have the function that adjust body disorders of lipid metabolism.
And glibenclamide positive drug group is compared for AST with ALT activity, there is different degrees of reduction, illustrates positive drug
Group for liver by damaging action, and yellow bur powder to liver then without damaging action, and there are apparent differences.
The yellow bur reality polysaccharide for reducing blood sugar effect experiment of embodiment 2
1) experimental drug：Yellow bur reality polysaccharide, is prepared by the following method：
A, yellow bur reality degreasing：Take the fruit 400g drying of ripe yellow thorn (i.e. straight fringe barberry), grinding and sieving；Take Huang
Bur mixes with petroleum ether in fact, and ultrasonic extraction after mixing is centrifuged off extracting solution, and residue filter volatilizes petroleum ether, obtains grease removal
Yellow bur reality residue；
B, yellow bur removes monosaccharide in fact：Grease removal Huang bur reality residue is mixed with ethyl alcohol, and the concentration of alcohol is 70~85%
V/v, refluxing extraction are centrifuged off extracting solution, ethyl alcohol are volatilized after residue filter, obtain and have removed monosaccharide Huang bur reality residue；
C, yellow thorn Thick many candies extraction：It is mixed monosaccharide Huang bur reality residue has been removed obtained by step B with pure water, Microwave-assisted Extraction
It takes, centrifugation obtains extracting solution, is discarded after residue filter；Extracting solution is concentrated to give Thick many candies extracting solution；
D, the purifying of yellow thorn Thick many candies：H is used in Thick many candies extracting solution successively2O2, decolourized, Savege methods remove egg
White matter, then dialyse and remove 3500Da or less small-molecule substances, the polysaccharide Aqueous extracts purified；
E, the preparation of yellow bur reality polysaccharide：It is molten that ethyl alcohol is added in purified polysaccharide Aqueous extracts concentrate obtained by step D
Liquid makes ethyl alcohol final concentration reach 75%v/v, amounts to 2.06g after gained precipitation is dry to get to yellow bur reality Polysaccharide B DP.
Anthrone-sulfuricacid method measurement is carried out to yellow bur reality polysaccharide prepared by the method for the present invention, wherein polyoses content is 93%
±2w/w.For dosage based on rat body weight, gavage low dosage, middle dosage, high dose are respectively 75mg/kg, 150mg/kg,
300mg/kg (to prepare in terms of gained polysaccharide quality)
2) animal：Four week old Kunming rats, male, regular grade, 135g are provided by Gansu Chinese of Traditional Chinese Medicine.In in laboratory
It is placed in raise in mouse cage and be tested after a week (per 8, cage, 22 ± 1 DEG C of temperature, humidity 42% ± 2%).It is divided into six groups：Point
Not Wei blank group (intravenous injection the non-modeling of citrate buffer solution, ultra-pure water gavage), (tail vein injection STZ modelings surpass model group
Pure water gavage), positive drug group (tail vein injection STZ modelings, glibenclamide 20mg/kg gavages), yellow bur reality polysaccharide low dosage
Group BDP-L (tail vein injection STZ modelings, yellow bur reality polysaccharide gavage 75mg/kg), yellow bur reality polysaccharide middle dose group BDP-M
(tail vein injection STZ modelings, yellow bur reality polysaccharide gavage 150mg/kg), yellow bur reality polysaccharide high dose group BDP-H (tail veins
Inject STZ modelings, yellow bur reality polysaccharide gavage 300mg/kg).Modeling method：Fasting 16h in advance then carries out STZ (lemons to it
Lemon acid buffer is prepared) tail vein injection 60mg/kg, after injection, surveys its blood glucose, testing model success or not for 72 hours.
3) experiment reagent：95% medicinal alcohol, Nanjing Ning Shi chemical reagent Co., Ltd；Physiological saline, Shandong all medicine together
Industry Co., Ltd；Superoxide dismutase (SOD), catalase (CAT)/glutathione peroxidase (GSH-PX), third
Bio tech ltd is built up in the Nanjing dialdehyde (MDA).
4) laboratory apparatus：Bio-Rad680 type microplate reader, Bio Rad Laboratories；DS-1 type tissue Syrup-homogenizing instruments, Shanghai are just intelligent
Trade Co., Ltd.；TGL-16G-A type tube centrifuges, Anting Scientific Instrument Factory, Shanghai；The visible uv-spectrophotometrics of UV-722N
Meter, Shanghai Ni Ke Co., Ltds；HD type thermostat water baths, Constant Temp. Instrument Factory, Liaoyang.
5) experimentation：Healthy SD rat 48 is taken, 6 groups is randomly divided by weight, is divided into blank group, model group, the positive
Medicine group and low, in, high dose group.Blank group and the daily gavage of model group give distilled water；The daily gavage of positive drug group gives lattice
Arrange this urea 1 time；Yellow bur reality polysaccharide is given to the daily gavage of test medicine group 1 time, and six groups are continuously given 28d, wherein every group
It keeps surveying a blood glucose every 7 days, and records；After last gives tested material, after anesthesia, blood is taken through arteria carotis, acquired blood from
The heart takes supernatant, for detecting last blood glucose and insulin.
6) yellow bur reality polysaccharide reduces the measurement and result of the blood glucose, insulin suffered from diabetes rat body
Influence (Mean ± SD) of the yellow bur reality polysaccharide of table 5 to the STZ rat blood sugars induced
Note：Compared with normal groupaP ＜ 0.05；Compared at modulebP ＜ 0.05；
Influence (Mean ± SD) of the yellow bur powder of table 6 to the STZ rat insulins induced
Note：Compared with normal groupaP ＜ 0.05；Compared at modulebP ＜ 0.05；
By table 5,6 it is found that present invention Huang each dosage group of bur reality polysaccharide has the blood glucose of the STZ diabetes rats induced
Reduction acts on, and adjusts effect of increasing to insulin, weight tool, and be in dose-effect relationship, high dose group has significantly with model group
Sex differernce (p ＜ 0.05).Meanwhile experiment shows that the effect of high dose group is suitable with the effect of glibenclamide, and glibenclamide is made
For chemically synthesized drug, there is certain side effect to body, therefore, it is suggested that real using the yellow bur extracted in natural plants
Polysaccharide can do alternative medicine use.
Experiment conclusion：Gavage is carried out to the diabetes rat that STZ is induced using yellow bur reality polysaccharide, suffers from diabetes rat
Blood glucose has reduction effect, adjusts effect of increasing to insulin, weight tool, and be in dose-effect relationship, high dose group and model
The significant difference (p ＜ 0.05) of group.Polysaccharide is soluble in hot water, is all had according to the real and yellow bur reality polysaccharide of yellow bur hypoglycemic
This result of effect can estimate without doubt, and the water extract of yellow bur reality also has hypoglycemic effect.
Claims (5)
- The purposes that 1. yellow bur is real or its extract is as sole active raw material in preparing hypoglycemic drug；The Huang, which pierces, is The straight fringe barberry Berberis dasystachya Maxim. of Berberidaceae plant；The yellow thorn fruit extract is that yellow bur is real Water extract.
- 2. purposes according to claim 1, it is characterised in that：The drug is to prevent or treat I types, type-2 diabetes mellitus Or/and the drug of diabetic complication.
- 3. purposes according to claim 1 or 2, it is characterised in that：The drug is the drug for improving insulin level.
- 4. purposes according to claim 1 or 2, it is characterised in that：The drug is peroral dosage form.
- 5. the purposes described in claim 4, it is characterised in that：The peroral dosage form be selected from tablet, capsule, pill, granule, Powder, extract or oral solution.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1260131A (en) * | 1999-09-20 | 2000-07-19 | 青海省青藏高原生物制品有限公司 | Reddish beautycherry jam |
| CN1362070A (en) * | 2000-12-30 | 2002-08-07 | 中国科学院西北高原生物研究所 | Composite medicine for treating hyperlipemia |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP5309292B2 (en) * | 2010-09-27 | 2013-10-09 | 沖縄県 | Lipase inhibitor |
-
2015
- 2015-06-09 CN CN201510323281.9A patent/CN104906163B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1260131A (en) * | 1999-09-20 | 2000-07-19 | 青海省青藏高原生物制品有限公司 | Reddish beautycherry jam |
| CN1362070A (en) * | 2000-12-30 | 2002-08-07 | 中国科学院西北高原生物研究所 | Composite medicine for treating hyperlipemia |
Non-Patent Citations (2)
| Title |
|---|
| 红珍珠降糖胶囊中微量元素营养研究;索有瑞等;《广东微量元素科学》;20001231;第7卷(第8期);第47-50页，尤其是第47页正文第1段，第48页倒数第2段及第49页倒数第2段 * |
| 红珍珠降糖胶囊功效成分多糖的分析;李天才等;《成都中医药大学学报》;20010630;第24卷(第2期);第38-39页，尤其是第38页左栏第1-2段及右栏第2段 * |
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