CN104971080B - Yellow bur reality polysaccharide improves the purposes of disorders of lipid metabolism - Google Patents
Yellow bur reality polysaccharide improves the purposes of disorders of lipid metabolism Download PDFInfo
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Abstract
The present invention provides purposes of the yellow bur reality polysaccharide in the drug, health care product or food that preparation improves disorders of lipid metabolism.The present invention can reduce cholesterol (TC), triglycerides (TG), low-density lipoprotein (LDL), increasing high density lipoprotein (HDL) studies have shown that yellow bur reality polysaccharide has the function of improvement disorders of lipid metabolism；And the relevant enzymatic activity of lipid metaboli can be increased and increase lipoproteinesterase and liver esterase activity, it can reduce glutamic-oxalacetic transaminease and gpt activity simultaneously, it is not damaged to hepatic tissue, it can be as drug, health care product or the food of the improvement disorders of lipid metabolism of good security.
Description
Technical field
The invention belongs to field of health care products, and in particular to yellow bur reality polysaccharide is in the drug that preparation improves disorders of lipid metabolism
Purposes.
Technical background
The metabolism of lipid include lipid small intestine it is digested, absorb, blood circulation is entered by lymphatic system and (is turned by lipoprotein
Fortune), it is converted through liver, is stored in adipose tissue, utilized when needing by tissue.The main function of lipid in vivo is that oxidation supplies
Can, adipose tissue is the energy warehouse of body, fat can also cooperate with skin, bone, muscle protection internal organ, prevent body temperature distribute and
Help the absorption of liposoluble vitamin in food.Phosphatide is the important feature ingredient of all cell membranes, and cholesterol is cholic acid and class
The precursor of steroid hormone (cortex hormone of aadrenaline and gonadal hormone).Lipid metabolism by heredity, neurohumor, hormone, enzyme and
The adjusting of the histoorgans such as liver.When these are because being known as abnormal, disorders of lipid metabolism (also referred to as disorders of lipid metabolism) can be caused
With the pathophysiological change in relation to organ, as hyperlipoprotememia, lipoidosis and its caused by clinical syndrome, obesity
Disease, ketoacidosis, fatty liver and scleredema neonatorum etc..
Huang thorn, the straight fringe barberry Berberis dasystachya Maxim. of Berberidaceae plant are (black with white thorn, sea-buckthorn
Thorn) it is equally traditional wild acinus that the ethnic groups such as illiteracy, hiding, Uygur are medicinal and eat, it is referred to as " thorn of Qinghai three ".It is " green
The common Chinese herbal medicine handbook in sea " in Cortex berberidis dasystachyae (i.e. root skin) is documented: Cortex berberidis dasystachyae is that dicotyledon medicine Berberidaceae is planted
The stem skin of the straight fringe barberry of object.Brief description has also been made in its fruit medicine function, has and treats abdominal pain, indigestion, abdominal distension,
The functions such as dysentery.
It yet there are no the report for improving dyslipidemias to yellow bur reality polysaccharide.
Summary of the invention
The purpose of the present invention is to provide the new applications of yellow bur reality polysaccharide.
Specifically, the present invention provides yellow bur reality polysaccharide improves drug, health care product or the food of disorders of lipid metabolism in preparation
Purposes in product.
Further, the drug, health care product or food are drug, the health care product for improving diabetic's disorders of lipid metabolism
Or food, it can be used to treatment hyperlipidemia with diabetes.
Wherein, the improvement disorders of lipid metabolism, which refers to, reduces diabetic's cholesterol, triglyceride or/and low density lipoprotein
Albumen, increasing high density lipoprotein.
Wherein, the diabetes are I type or type-2 diabetes mellitus.
Further, the diabetic is the normal or impaired patient of liver function.The research of the invention finds that dative arranges
Unlike the chemical drugs such as this urea, yellow bur reality polysaccharide is harmless to liver function, therefore, yellow bur reality polysaccharide can be used for liver function
The treatment of energy impaired subjects.
Wherein, the drug, health care product or food are peroral dosage form；Further, the peroral dosage form is selected from tablet, glue
Wafer, pill, granule, powder, extract or oral solution.
Huang bur reality polysaccharide of the present invention, is the straight fringe barberry Berberis dasystachya of Berberidaceae plant
Maxim. the extract of fruit, polysaccharide is main component in extract.Measurement shows extract in the specific embodiment of the invention
Middle polyoses content is 93 ± 2%w/w, and the purity of polysaccharide is higher, carries out biological activity test, result with the polysaccharide under this purity
The bioactivity that can really reflect yellow bur reality polysaccharide efficiently avoids other compositions and does to active polysaccharide measurement
It disturbs.On the basis of known polysaccharide bioactivity, content of the polysaccharide in extract is even reduced, as long as appropriate adjust is extracted
Object dosage can equally play the same or similar bioactivity so that polysaccharide reaches corresponding action concentration.Therefore, originally
Invention limits, and polyoses content should be 60~95%w/w in extract.
Further, polyoses content is 80~95%w/w in extract, is further 90~95%w/w.
Wherein, the yellow bur reality polysaccharide is prepared using water extraction and alcohol precipitation method.
Further, in alcohol precipitation step, ethyl alcohol final concentration reaches 60~85%v/v, is further 70~80%
V/v, preferably 75 ± 2%v/v.
Further, the water extraction and alcohol precipitation method concrete operations are as follows: after yellow bur reality degreasing, removing monosaccharide, extracting in water,
Aqueous extracts decoloration, removing protein, dialysis are except after small molecule, addition ethyl alcohol to alcohol content reaches 60~85%v/v, stands, to be precipitated
Completely, precipitating is taken, is dried to get yellow bur reality polysaccharide is arrived.
Further, dialyse removing small molecule molecular weight in 3500Da or less.
Further, degreasing concrete operations are as follows: after yellow bur is crushed in fact, sieving, use petroleum ether or ether for
Extraction solvent, the residue filter after extraction, volatilizes solvent, obtains degreasing Huang bur reality residue.
Further, go the concrete operations of monosaccharide as follows: gained degreasing Huang bur reality residue, with 70~85%v/v
Ethyl alcohol extracts, and the residue after extraction volatilizes solvent to get monosaccharide Huang bur reality residue has been removed；Preferred concentration of alcohol is 80%v/
v。
Further, dialysis is except ethyl alcohol is added after small molecule to alcohol content to 70~80%v/v, preferably 75 ± 2%
v/v。
By above-mentioned purification step, the purity of yellow bur reality polysaccharide can effectively improve.Certainly, if it is only necessary to prepare
The lower yellow bur reality polysaccharide of purity, can also omit one or more in other purification steps in addition to alcohol precipitation.
In addition, the decolorization for polysaccharide, usually (such as using ion-exchange (such as DEAE- cellulose), oxidizing process
H2O2), absorption method (such as active carbon), in actual use can according to self-demand, in conjunction with later period polysaccharide yield and
Purity selectes suitable discoloration method, selects H in a specific embodiment of the invention2O2Decoloration, but this is not limited to this hair
It is bright to use such decoloration mode.
For removing protein, common method has trichloroacetic acid method, hydrochloric acid method, Sevage method (also on the books for Sevag
Method) etc., removing of being suitble to can be selected in conjunction with the yield and purity of later period polysaccharide according to self-demand in actual use
Protein process selects Sevage method in a specific embodiment of the invention, but this is not limited to the present invention and can only use
Such removing protein mode.
Wherein, the yellow thorn is the straight fringe barberry Berberis dasystachya Maxim. of Berberidaceae plant.
The present invention can reduce cholesterol studies have shown that yellow bur reality polysaccharide has the function of improvement disorders of lipid metabolism
(TC), triglycerides (TG), low-density lipoprotein (LDL), increasing high density lipoprotein (HDL)；And lipid metaboli phase can be increased
The enzymatic activity of pass increases lipoproteinesterase and liver esterase activity, while can reduce glutamic-oxalacetic transaminease and glutamic-pyruvic transaminase work
Property, it is not damaged to hepatic tissue, it can be as drug, health care product or the food of the improvement disorders of lipid metabolism of good security.
Specific embodiment
Huang bur reality polysaccharide used in the specific embodiment of the invention is using conventional Polyose extraction, way of purification preparation
It obtains, concrete operations are as follows in the present invention:
Embodiment 1: the preparation of yellow bur reality polysaccharide
1) the fruit 400g drying of mature yellow thorn (i.e. straight fringe barberry), grinding and sieving yellow bur reality degreasing: are taken；Take Huang
Bur mixes with petroleum ether in fact, and ultrasonic extraction after mixing is centrifuged off extracting solution, and residue filter volatilizes petroleum ether, obtains grease removal
Yellow bur reality residue；
2) yellow bur removes monosaccharide in fact: grease removal Huang bur reality residue is mixed with ethyl alcohol, and the concentration of alcohol is 70~85%
V/v, refluxing extraction are centrifuged off extracting solution, volatilize ethyl alcohol after residue filter, obtain and have removed monosaccharide Huang bur reality residue；
3) yellow thorn Thick many candies extract: it is mixed monosaccharide Huang bur reality residue has been removed obtained by step 2) with pure water, it is microwave-assisted
It extracts, centrifugation obtains extracting solution, discards after residue filter；Extracting solution is concentrated to get Thick many candies extracting solution；
4) H successively the purifying of yellow thorn Thick many candies: is used in Thick many candies extracting solution2O2, decolourized, Savege method removes egg
White matter, then dialyse and remove 3500Da or less small-molecule substance, the polysaccharide Aqueous extracts purified；
5) it is molten that ethyl alcohol the preparation of yellow bur reality polysaccharide: is added in the resulting purified polysaccharide Aqueous extracts concentrate of step 4)
Liquid makes ethyl alcohol final concentration reach 75%v/v, amounts to 2.06g after gained precipitating is dry to get to Huang bur reality Polysaccharide B DP.
Embodiment 2: the preparation of yellow bur reality polysaccharide
1) the fruit 400g drying of mature yellow thorn (i.e. straight fringe barberry), grinding and sieving yellow bur reality degreasing: are taken；Take Huang
Bur mixes with petroleum ether in fact, and refluxing extraction is centrifuged off extracting solution, and residue filter volatilizes petroleum ether, obtains grease removal Huang bur
Real residue；
2) yellow bur removes monosaccharide in fact: grease removal Huang bur reality residue is mixed with ethyl alcohol, and the concentration of alcohol is 70~85%
V/v, ultrasonic extraction are centrifuged off extracting solution, volatilize ethyl alcohol after residue filter, obtain and have removed monosaccharide Huang bur reality residue；
3) yellow thorn Thick many candies extract: it is mixed monosaccharide Huang bur reality residue has been removed obtained by step 2) with pure water, temperature extraction takes,
Centrifugation obtains extracting solution, discards after residue filter；Extracting solution is concentrated to get Thick many candies extracting solution；
4) H successively the purifying of yellow thorn Thick many candies: is used in Thick many candies extracting solution2O2, decolourized, Savege method removes egg
White matter, then dialyse and remove 3500Da or less small-molecule substance, the polysaccharide Aqueous extracts purified；5) system of yellow bur reality polysaccharide
It is standby: ethanol solution is added in the resulting purified polysaccharide Aqueous extracts concentrate of step 4), ethyl alcohol final concentration is made to reach 80%v/
V, gained precipitating is dry to amount to 1.355g to get to Huang bur reality Polysaccharide B DP.
Embodiment 3: the preparation of yellow bur reality polysaccharide
1) the fruit 400g drying of mature yellow thorn (i.e. straight fringe barberry), grinding and sieving yellow bur reality degreasing: are taken；Take Huang
Bur mixes with petroleum ether in fact, it is ultrasonic after refluxing extraction again, be centrifuged off extracting solution, residue filter volatilizes petroleum ether, obtains and has removed
Rouge Huang bur reality residue；
2) yellow bur removes monosaccharide in fact: grease removal Huang bur reality residue is mixed with ethyl alcohol, and the concentration of alcohol is 70~85%
V/v, ultrasonic extraction are centrifuged off extracting solution, volatilize ethyl alcohol after residue filter, obtain and have removed monosaccharide Huang bur reality residue；
3) yellow thorn Thick many candies extract: it is mixed monosaccharide Huang bur reality residue has been removed obtained by step 2) with pure water, refluxing extraction,
Centrifugation obtains extracting solution, discards after residue filter；Extracting solution is concentrated to get Thick many candies extracting solution；
4) H successively the purifying of yellow thorn Thick many candies: is used in Thick many candies extracting solution2O2, decolourized, Savege method removes egg
White matter, then dialyse and remove 3500Da or less small-molecule substance, the polysaccharide Aqueous extracts purified；
5) it is molten that ethyl alcohol the preparation of yellow bur reality polysaccharide: is added in the resulting purified polysaccharide Aqueous extracts concentrate of step 4)
Liquid makes ethyl alcohol final concentration reach 70%v/v, and gained precipitating is washed 3 times with 20mL dehydrated alcohol, and the sediment after washing carries out
Spray drying, so that moisture content of material, which is down to 10%, amounts to 1.67g hereinafter, obtaining yellow bur reality Polysaccharide B DP.
Anthrone-sulfuricacid method measurement is carried out to the yellow bur reality polysaccharide of the method for the present invention preparation, wherein polyoses content is 93%
±2w/w。
The present invention yellow bur reality polysaccharide dosage used in the following embodiment, with the calculating of polyoses extract gross mass.
The yellow bur reality polysaccharide of embodiment 4 improves disorders of lipid metabolism effect experiment
1) experimental drug: yellow bur reality polysaccharide, for present invention preparation gained, as white powder solid.Dosage is by big
Mouse batheroom scale, stomach-filling low dosage, middle dosage, high dose are respectively 75mg/kg, 150mg/kg, 300mg/kg,
2) animal: four week old Kunming rats, male, regular grade, 135g are provided by Gansu Chinese of Traditional Chinese Medicine.In in laboratory
It is placed in raise in mouse cage and is tested (8, every cage, 22 ± 1 DEG C of temperature, humidity 42% ± 2%) after a week.It is divided into six groups: point
Not Wei blank group (intravenous injection the non-modeling of citrate buffer solution, ultrapure water stomach-filling), (tail vein injection STZ modeling surpasses model group
Pure water stomach-filling), positive drug group (tail vein injection STZ modeling, glibenclamide 20mg/kg stomach-filling), yellow bur reality polysaccharide low dosage
Group (tail vein injection STZ modeling, yellow bur reality polysaccharide stomach-filling 75mg/kg), yellow bur reality polysaccharide middle dose group (tail vein injection
STZ modeling, yellow bur reality polysaccharide stomach-filling 150mg/kg), yellow bur reality polysaccharide high dose group (tail vein injection STZ modeling, Huang thorn
Fruit polysaccharide stomach-filling 300mg/kg).Modeling method: fasting 16h in advance then carries out STZ (citrate buffer solution preparation) to it
Tail vein injection 60mg/kg after injection, surveys the indexs such as its blood glucose, blood lipid, testing model success or not for 72 hours.
3) experiment reagent: 95% medicinal alcohol, Nanjing Ning Shi chemical reagent Co., Ltd；Physiological saline, Shandong all medicine together
Industry Co., Ltd；Cholesterol, triglyceride, high-density lipoprotein, density lipoprotein, glutamic-oxalacetic transaminease, glutamic-pyruvic transaminase, rouge
Albumen esterase, liver esterase kit, Biotechnology Co., Ltd is built up in Nanjing.
(4) laboratory apparatus: Bio-Rad680 type microplate reader, Bio Rad Laboratories；DS-1 type tissue Syrup-homogenizing instrument, Shanghai are just intelligent
Trade Co., Ltd.；TGL-16G-A type tube centrifuge, Anting Scientific Instrument Factory, Shanghai；The visible uv-spectrophotometric of UV-722N
Meter, Shanghai Ni Ke Co., Ltd；HD type thermostat water bath, Constant Temp. Instrument Factory, Liaoyang.
(5) experimentation: taking healthy SD rat 48, is randomly divided into 6 groups by weight, is divided into blank group, model group, the positive
Medicine group and low, in, high dose group.Distilled water is given in blank group and the daily stomach-filling of model group；Lattice are given in the daily stomach-filling of positive drug group
It arranges this urea 1 time；It is given yellow bur reality polysaccharide 1 time to the daily stomach-filling of test medicine group, six groups are continuously given 28d；Last is given
After tested material, after anesthesia, blood is taken through arteria carotis, acquired blood centrifuging and taking supernatant turns for detecting blood glucose, blood lipids index and millet straw
Adnosine deaminase, glutamic-pyruvic transaminase.To its death, rat liver tissue is taken, surface blood stains is washed away with physiological saline, is blotted with filter paper,
Tissue Syrup-homogenizing instrument is homogenized, and collects homogenate into centrifuge tube, is centrifuged 10min with 3000rpm, Aspirate supernatant carries out rouge
Albumen esterase and liver esterase determination of activity.
(6) the intracorporal blood glucose of diabetes rat, lipid determination result are suffered from yellow bur reality polysaccharide reduction
The diabetes of STZ induction can make rat Islet cells occur karyopycnosis and hyaloid lesion, lipid metaboli with
Get muddled.The lipid metaboli of rat gets muddled simultaneously, and related blood lipids index and the relevant enzymatic activity of lipid metaboli also occur disorderly
Disorderly.The present invention is simultaneously determined blood lipid and blood glucose, as a result part table 1,2.
Influence (Mean ± SD) of the yellow bur reality polysaccharide of table 1 to the STZ rat blood sugar induced
Note: compared with normal groupaP < 0.05；Compared at mould groupbP < 0.05；
Rat Cholesterol TC, the triglycerides TG, high-density lipoprotein that the yellow bur reality polysaccharide component of table 2 induces STZ
The influence (Mean ± SD) of HDL, low-density lipoprotein LDL
Note: compared with normal groupaP < 0.05；Compared at mould groupbP < 0.05
By table 1,2 it is found that in above-mentioned experiment STZ induce rat blood sugar, blood lipid increase, show above-mentioned experiment modeling at
Function.Huang each dosage group of bur reality polysaccharide of the invention is to the cholesterol of the STZ disorders of lipid metabolism rat induced, triglycerides, low close
Degree lipoprotein, which has, adjusts reduction effect, has adjusting effect of increasing to high-density lipoprotein, and be in dose-effect relationship, high dose
Group has significant difference (p < 0.05) with model group.Meanwhile experiment shows the effect of high dose group and the effect of glibenclamide
Quite, and glibenclamide has certain side effect to body as chemically synthesized drug, therefore, it is suggested that being planted using natural
The yellow bur reality polysaccharide extracted in object can do alternative medicine use.
(7) to the influence of relevant enzyme
Liver esterase HL is that the one kind synthesized by hepatic parenchymal cells has phosphorus A1 and triglyceride hydrolytic enzyme activities, to plasma adiponectin
Matter transports the extracellular protein to play an important role, is primarily involved in the reconstruct of HDL-C and the generation of chylomicron remains, low-density lipoprotein
It thanks and the antiport of cholesterol.HL vigor is abnormal to have higher correlation with atherosclerosis and diabetes.Lipoprotein
Esterase LPL is the synthesis of the parenchymas such as fat cell, cardiac muscle cell, Skeletal Muscle Cell, mammary glandular cell and macrophage and divides
A kind of glycoprotein secreted.Active LPL exists in the form of homodimer, passes through electrostatic attraction and capillary endothelial cell surface
Polysaccharide combine, heparin can promote the LPL of this combining form to be released into blood, and its activity can be improved.LPL is lipoprotein generation
The key enzyme thanked is key enzyme of the hydrolysis rich in triglyceride in three casein of glycerol.Its major function is hydrolysis CM and VLDL
In triglyceride be glycerol and fatty acid.Lipoproteinesterase LPL and liver esterase HL can decompose triglycerides TG and decompose
For glycerol and free fatty acid FFA, rouge can be calculated separately out by measuring its free fatty acid for decomposing generation using copper reagent
The activity of albumen esterase and liver esterase.
Under the stimulation of some drugs, internal hepatic tissue is invaded profit by fat cell, influences correlation for AST and ALT content
Hepatocyte enzyme activity, and under normal circumstances, as Simvastatin and glibenclamide etc. can all have a certain impact to hepatic tissue.
Rat lipoproteinesterase LPL, the liver esterase HL, glutamic-oxalacetic transaminease that the yellow bur reality polysaccharide component of table 3 induces STZ
The influence (Mean ± SD) of AST, glutamic-pyruvic transaminase ALT
Note: compared with normal groupaP < 0.05；Compared at mould groupbP < 0.05；Compared with normal groupcP < 0.05.
Yellow bur reality polysaccharide can be such that HL the and LPL activity of experimental rat increases, and LPL is to remove the limit for being rich in TG lipoprotein
Fast enzyme, to play its important function in lipoprotein circulation.The HL activity of diabetes rat can be made to increase simultaneously, thus
By HL as ligand, promotes liver cell intake cholesterol and chylomicron remnant, reduce total cholesterol and glycerol three in blood plasma
Vinegar concentration, and then have the function that adjust body disorders of lipid metabolism.
And glibenclamide positive drug group is compared for AST with ALT activity, there is different degrees of reduction, illustrates positive drug
Group has damage liver, and yellow bur reality polysaccharide to liver then without damaging action, and there are apparent differences.
Experiment conclusion: blood lipids index and the esterase active of the diabetes rat through STZ induction are it is demonstrated experimentally that yellow bur is real more
Sugar has very strong improvement disorders of lipid metabolism activity.The diabetes that Huang each dosage group of bur reality polysaccharide of the invention induces STZ are big
Cholesterol, triglycerides, the low-density lipoprotein of mouse, which have, adjusts reduction effect, is made to high-density lipoprotein by adjusting to rise
With.And be in dose-effect relationship, high dose group and model group have significant difference (p < 0.05)；Yellow bur reality polysaccharide can make
HL the and LPL activity for suffering from the liver of diabetes rat increases, and is in dose-effect relationship, and high dose group has conspicuousness poor with model group
Different (p < 0.05)；Meanwhile it is demonstrated experimentally that AST with ALT activity compares glibenclamide positive drug group, there is different degrees of drop
It is low, illustrate positive drug group for liver by damaging action, and yellow bur reality polysaccharide to liver then without damaging action.Therefore, yellow
Bur reality polysaccharide can be applied to improve food, health care product or the drug of disorders of lipid metabolism effect.
Claims (17)
1. purposes of the yellow bur reality polysaccharide as sole active agent in the drug or health care product that preparation improves disorders of lipid metabolism；
The yellow thorn is the straight fringe barberry Berberis dasystachya Maxim. of Berberidaceae plant.
2. purposes according to claim 1, it is characterised in that: the drug or health care product are to improve diabetic's rouge generation
Thank the drug or health care product of disorder.
3. purposes according to claim 1, it is characterised in that: the improvement disorders of lipid metabolism, which refers to, reduces diabetic
Cholesterol, triglyceride or/and low-density lipoprotein, increasing high density lipoprotein.
4. purposes according to claim 2, it is characterised in that: the diabetes are I type or type-2 diabetes mellitus.
5. purposes according to claim 2, it is characterised in that: the diabetic is the normal or impaired trouble of liver function
Person.
6. purposes described in any one according to claim 1~5, it is characterised in that: the drug or health care product are oral agents
Type.
7. purposes according to claim 6, it is characterised in that: the peroral dosage form be selected from tablet, capsule, pill,
Granula, powder, extract or oral solution.
8. purposes according to claim 1, it is characterised in that: purity of polysaccharide 60-95%w/w.
9. purposes according to claim 8, it is characterised in that: purity of polysaccharide is 80~95%w/w.
10. purposes according to claim 9, it is characterised in that: purity of polysaccharide is 90~95%w/w.
11. according to claim 1~5, purposes described in 7~9 any one, it is characterised in that: the Huang bur reality polysaccharide is adopted
It is prepared with water extraction and alcohol precipitation method.
12. purposes according to claim 11, it is characterised in that: the water extraction and alcohol precipitation method concrete operations are as follows: Huang is pierced
Fruit degreasing, after removing monosaccharide, extracting in water, Aqueous extracts decoloration, removing protein, dialysis are except after small molecule, being added ethyl alcohol to alcohol content
Reach 60~85%v/v, stands, it is to be precipitated to take precipitating completely, it is dry to get to Huang bur reality polysaccharide.
13. purposes according to claim 12, it is characterised in that: the small molecule molecular weight for removing of dialysing 3500Da with
Under.
14. purposes according to claim 12 or 13, it is characterised in that: ethyl alcohol is added to alcohol content after removing small molecule in dialysis
To 70~80%v/v.
15. purposes according to claim 14, it is characterised in that: dialysis is except ethyl alcohol is added after small molecule to alcohol content to 75
± 2%v/v.
16. purposes according to claim 12, it is characterised in that: degreasing concrete operations are as follows: after yellow bur is crushed in fact,
Sieving, uses petroleum ether or ether for Extraction solvent, the residue filter after extraction volatilizes solvent, and it is residual in fact to obtain degreasing Huang bur
Slag；
Go the concrete operations of monosaccharide as follows: gained degreasing Huang bur reality residue, with the extraction of 70~85%v/v ethyl alcohol, after extraction
Residue volatilize solvent to get monosaccharide Huang bur reality residue has been removed.
17. purposes according to claim 16, it is characterised in that: go in the concrete operations of monosaccharide, concentration of alcohol be 80 ±
2%v/v.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1362070A (en) * | 2000-12-30 | 2002-08-07 | 中国科学院西北高原生物研究所 | Composite medicine for treating hyperlipemia |
| WO2012093973A2 (en) * | 2011-01-06 | 2012-07-12 | Mahmut Bilgic | Stable acarbose formulations |
-
2015
- 2015-06-09 CN CN201510323285.7A patent/CN104971080B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1362070A (en) * | 2000-12-30 | 2002-08-07 | 中国科学院西北高原生物研究所 | Composite medicine for treating hyperlipemia |
| WO2012093973A2 (en) * | 2011-01-06 | 2012-07-12 | Mahmut Bilgic | Stable acarbose formulations |
Non-Patent Citations (2)
| Title |
|---|
| 红珍珠降糖胶囊功效成分多糖的分析;李天才等;《成都中医药大学学报》;20010630;第24卷(第2期);第38-39页，尤其是第38页左栏第1-2段 * |
| 红珍珠降糖胶囊的开发研究;索有瑞等;《青海科技》;20011231(第1期);第23-27页，尤其是第23页正文第1段 * |
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