CN104900290B - Device and method for realizing photoinduced rotation based on double-beam light trap - Google Patents
Device and method for realizing photoinduced rotation based on double-beam light trap Download PDFInfo
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Abstract
The invention relates to a device and method for realizing photoinduced rotation based on double-beam light trap, belonging to the technical field of optical micro-control systems. The device is composed of two light beam adjusting systems, a sample cell, an illumination system and a rotating speed measuring system. The two light beam adjusting systems respectively generate leftward-rotation circularly polarized light and rightward-rotation circularly polarized light which are the same in power but different in wavelengths, the two beams of circularly polarized light are input at the two sides of the sample cell and aligned to capture and rotate particles of birefringence material, the illumination system under the sample cell provides illumination, and the rotating speed measuring system over the sample cell is used to observe and measure the rotating speed of the particles of the birefringence material. The device and method have the advantages of higher control precision, higher rotating speed of the particles and higher stability.
Description
Technical field
The invention belongs to optical micro-manipulation systems technology field, particularly to one kind, photic rotation is realized based on dual-beam ligh trap
The device and method turning.
Background technology
The angular motion of meso-scale particulate is one of basic problem in physics and hydrodynamics, controls meso-scale
The angular motion of particulate is for the essence of material property in the research of biological cell, large biological molecule and nano-motor and microscopic fields
Really measurement has very important application.And the particulate under this yardstick is difficult to realize the manipulation of angular motion with Measure macro.
Photic rotation is that the meso-scale particulate growing up on the basis of optical tweezer angularly manipulates technology, and it utilizes luminous power
Or moment realize particulate rotation control.It is known that photon also has angular momentum in addition to carrying linear momentum, light is through birefringence
After rate particulate, the change of photon angular momentum can pass to particulate and produce rotation.R.A.Beth in 1936 hanged using filament half
Wave plate experimentally observed photic rotation phenomenon for the first time, and hereafter people are exploring the method for photic rotation always and its answering
With.The appearance of optical tweezer promotes the development of photic spin theory and technology, because optical tweezer can realize the position of meso-scale particulate
Put control, and it can be made no longer to be subject to the effect of other machinery frictional force so that photic rotation is experimentally easier to realize.Due to light
On the basis of cause is rotated in the manipulation of optical tweezer original three-dimensional position, increased the manipulation to the angular dimension of captured particulate, in nanometer
Science and technology and technical field of biology have a good application prospect, and are widely used in micromechanics motor, the stablizing of mcroorganism molecule
Observe and the many aspects such as manipulation, measurement of the coefficient of viscosity.
Study and apply more photic spinning solution mainly to include angular momentum TRANSFER METHOD, dual-beam misalignment method, light at present
Field control method, rotation spot method, light field scattering force promote class windmill particulate rotary process etc..Its essence is the birefringence using particulate
Characteristic, absorption characteristic or its special shape capture spin angular momentaum, orbital angular momentum or the linear momentum of laser to change, to reach
Promote the purpose of particulate rotation using light field.Realize photic rotation currently with dual-beam ligh trap and be all based on dual-beam misalignment
Method.
The photic rotation of dual-beam misalignment method refers to lose when two bundle capture laser transmit in opposite directions and exist in dual-beam ligh trap
On time, there is the effect of moment in trapping stiffness to captured particulate, so that particulate is rotated.Dual-beam misalignment method is to being steered particulate
Material, shape all unrestricted, be with a wide range of applications, but the method have the disadvantages that：(1) misalignment distance is difficult to control
System, when misalignment apart from excessive when it is impossible to stably capture particulate, when misalignment apart from too small when, rotary speed very little；(2) the method
Photic rotation is realized it is impossible to the high speed realizing particulate stably rotates by the transmission of linear momentum；(3) optical imaging techniques do is utilized to measure
Particulate rotating speed, certainty of measurement is relatively low.
Spin angular momentaum TRANSFER METHOD utilizes the photic rotation realized that interacts of circularly polarized light and birefringence particulate, permissible
Obtain very high rotating speed, and it is also more convenient to produce the mode of rotation, has a good application prospect.Currently with spin angular motion
Amount TRANSFER METHOD is realized photic rotation and is all based on single beam gradient force ligh trap (optical tweezer) system, has as a drawback that：(1) single beam
Photic rotation technique needs the laser beam of high order focusing to form larger gradient force, easily damages active somatic cell, is unfavorable for living
Somatic rotation manipulation；(2) operating distance of the photic rotation technique of single beam is limited by condenser lens focal length, effective working distance
From short, operation inconvenience；(3) rotary speed measurement is low with control accuracy.
Content of the invention
The principle of the present invention is as follows：The present invention is based on dual-beam ligh trap, realizes being situated between using spin angular momentaum TRANSFER METHOD and sees chi
The high speed of degree particulate stably rotates.Captured particulate is the birefringent material particulate of meso-scale, and birefringent material refers to make
There is birefringence and resolve into the crystalline material of the two bundle polarised lights in different polarization direction, including quartz, calcite, red treasured in light wave
Stone etc..Two bundle capture laser are respectively left circularly polarized light and the right-circularly polarized light of different wave length, when two bundle circularly polarized lights are made
During for birefringent material particulate, the spin angular momentaum of photon itself changes, according to the law of conservation of momentum, the changing of angular momentum
Change passes to birefringent material particulate makes particulate rotate.
The technical solution used in the present invention is：A kind of device realizing photic rotation based on dual-beam ligh trap, this device by
Two sets of beam adjustment system, sample cell, illuminator and five parts of speed measuring system form, wherein two sets light beam regulation systems
System produces the left-handed and right-circularly polarized light that power is identical, wavelength is different respectively, and described two bundle circularly polarized lights are from transparent sample pond
Both sides input and are aligned, and penetrate particles of material for capture and rotating birefringence, utilize illuminator to provide illumination below sample cell,
And observed and measured the rotary speed of birefringent material particulate above sample cell using speed measuring system.
Wherein beam adjustment system is made up of laser instrument, λ/2 wave plate, polarizing beam splitter mirror and λ/4 wave plate, laser emitting
Continuous laser forms linearly polarized light, laser emitting laser in two sets of beam adjustment system after λ/2 wave plate and polarizing beam splitter mirror
Wavelength should difference, the optical axis adjusting described λ/2 wave plates is used for changing the polarization state inciding polarizing beam splitter mirror laser, thus
Reach the purpose adjusting polarizing beam splitter mirror outgoing linearly polarized light light intensity, described linearly polarized light through λ/4 wave plate, about adjusting
The optical axis of λ/4 wave plate described in two sets of beam adjustment system make its angle with described linearly polarized light polarization direction be respectively ±
45 °, so that shoot laser is changed into left-handed and right-circularly polarized light respectively, described left-handed and right-circularly polarized light are input to sample
As capture laser in pond, birefringent material particulate is captured and is rotated control.
Why left and right two bundle capture laser is respectively left-handed and right-circularly polarized light, and this is by be captured and rotating birefringence
The relation of the direction of rotation of particles of material and circularly polarized light polarization direction of penetrating determines, left-handed can lead respectively with right-circularly polarized light
Cause the clockwise of birefringent material particulate and rotate counterclockwise, utilize left-handed and right-circularly polarized light therefore in dual-beam ligh trap
Simultaneously rotating birefringence penetrates the purpose that particles of material can reach raising particulate rotary speed for capture.
Sample cell is the transparent glass container being mounted with birefringent material particulate, and illuminator by LED/light source and focuses on thoroughly
Microscope group becomes, and positioned at the lower section of sample cell, its Main Function is to provide illumination for sample cell.
Speed measuring system by object lens, double-colored phase optical filtering, ccd image sensor, λ/4 wave plate, polarizing beam splitter mirror, two
Lens and two photodetector compositions, collect capture light through birefringent material particulate using the object lens above sample cell
The scattered beam being formed after scattering and the illuminating bundle after illuminator passes through sample cell, and will using double-colored phase optical filtering
Separately, wherein illuminating bundle is input in the ccd image sensor above object lens so that right for scattered beam and illuminating bundle
The capture of birefringent material particulate and rotary course are observed, and scattering envelope contains left-handed and right-circularly polarized light, wherein left-handed
Change with the rotation of birefringent material particulate with right-circularly polarized light proportion, scattered light is after double-colored phase optical filtering
The direction of propagation turn 90 degrees partially, through λ/4 wave plate, left-handed and right-circularly polarized light is changed into the orthogonal line in two bundle polarization directions
Polarised light, recycles polarizing beam splitter mirror by the two orthogonal linearly polarized lights in bundle polarization direction separately, is changed into polarization direction constant
But the mutually perpendicular two bunch polarised lights in the direction of propagation, every bunch polarised light, all after lens focus, uses photodetector respectively
Detected, the light intensity signal that two photodetectors are detected is divided by, the period of change of gained signal is exactly birefringence material
The rotation period of material particulate, the rotating speed of birefringent material particulate is exactly the inverse of its rotation period.
The present invention also provide a kind of the photic method rotating and measuring its rotating speed is realized based on dual-beam ligh trap, the method
Comprise the following steps that：
Step one, assemble and aforementioned the device of photic rotation realized based on dual-beam ligh trap and adjusts two sets of left and right light beams tune
Section system is so that two sets of beam adjustment system distinguish the left-handed and right-circularly polarized lights that emergent power is identical, wavelength is different；
Step 2, by two bundle circularly polarized lights be input in sample cell and be aligned, capture and rotating birefringence penetrate particulate, and by
Ccd image sensor in beam lighting system and speed measuring system is observed；
Step 3, using speed measuring system measure particulate rotary speed：The ratio of two photodetector light intensity signals
Period of change be the rotation period of birefringent material particulate, the rotating speed of birefringent material particulate is exactly falling of its rotation period
Number；
Step 4, the light intensity changing two bundle capture light using two sets of beam adjustment system and polarization state, thus change micro-
The position of grain and rotary speed, and the situation of change of the rotary speed of particulate is observed using speed measuring system.
The wavelength of two bundle capture laser is all near infrared band, and this is because near-infrared is to the capture rate seeing particulate that is situated between
Preferably, but simultaneously the wavelength of two bundle capture light should difference because can interfere when two bundle capture optical wavelength are identical, from
And affect the capture of particulate.
The rotary speed of particulate changes with the light intensity magnitude of two bundle capture light and the change of polarization state, it is possible to use light
Bundle regulating system changes light intensity and the polarization state of capture light, to realize the precision control to particulate rotary speed.Additionally, also may be used
With the relative size by changing two bundle capture light light intensity, control movement on optical axis for the particulate.
Compared with rotation photic with dual-beam misalignment method, advantage of the invention is that：
1. the present invention realizes photic rotation using spin angular momentaum TRANSFER METHOD, can be by changing the power of two bundle capture light
To control particulate rotating speed with polarization state, control accuracy is higher.
2. the present invention realizes the photic rotation of particulate using the transmission of spin angular momentaum, and the rotating speed of particulate faster, and is stablized
Property is more preferable.
3. the present invention is sent out with particulate rotation by measuring left-handed in Particle Scattering light and right-circularly polarized light proportion
Raw cyclically-varying measurement particulate rotating speed, the precision of tachometric survey is higher.
Compared with rotation photic with single beam spin angular momentaum TRANSFER METHOD, advantage of the invention is that：
1. the present invention realizes photic rotation using dual-beam optical trapping technique, mainly realizes particulate using the scattering force of capture light
Capture and rotation it is not necessary to high order focusing process is carried out to laser beam, not easy damaged active somatic cell.
2. the present invention realizes photic rotation using dual-beam optical trapping technique, can be by changing the power ratio of two bundle capture light
Captured particulate is controlled to move on optical axis, effective working distance is long, simple to operation.
3. the photic rotation technique based on dual-beam ligh trap, on the basis of original single beam controls, can control simultaneously
The power of two bundle capture light and polarization state, control accuracy is high, and is conducive to the measurement of particulate rotary speed.
Brief description
Fig. 1 is the structure drawing of device realizing photic rotation based on dual-beam ligh trap.
Specific embodiment
The present invention is further illustrated below in conjunction with the accompanying drawings, but should not therefore limit the scope of the invention.
With reference to Fig. 1, the device realizing photic rotation based on dual-beam ligh trap of the present invention, by a beam adjustment system
100th, No. two beam adjustment system 200, illuminator 300, sample cell 400 and speed measuring system 500 form.Two sets of light beams are adjusted
Section system 100, the 200 left-handed and right-circularly polarized light that power output is equal, wavelength is different respectively, by this left-handed and dextrorotation of two bundles
Circularly polarized light is input in sample cell 400 and is aligned as capture light, penetrates particles of material for capture and rotating birefringence.In sample
Utilize illuminator 300 to provide illumination below pond, and observed using speed measuring system 500 above sample cell and measure
The rotary speed of particulate.
A described beam adjustment system 100 is by laser instrument 101, λ/2 wave plate 102, polarizing beam splitter mirror 103, λ/4 wave plate
104 compositions.The capture laser of laser instrument 101 transmitting forms linearly polarized light after λ/2 wave plate 102 and polarizing beam splitter mirror 103, adjusts
The optical axis of described λ/2 wave plate 102 is used for changing the polarization state inciding polarizing beam splitter mirror 103 laser, thus reaching regulation partially
The purpose of beam splitter 103 outgoing linearly polarized light light intensity of shaking, by the linearly polarized light of outgoing through λ/4 wave plate 104, adjusts λ/4 wave plate 104
Emergent light is made to be changed into left circularly polarized light.
Described No. two beam adjustment system 200 are by laser instrument 201, λ/2 wave plate 202, polarizing beam splitter mirror 203, λ/4 wave plate
204 compositions.The device of No. two beam adjustment system 200 and a beam adjustment system 100 are identical, but laser instrument 201 outgoing swashs
Optical wavelength should be different from the shoot laser wavelength of laser instrument 101 in a beam adjustment system 100, in case two bundle capture light occur
The capture of interference effect birefringent material particulate, and its emergent light is right-circularly polarized light.Adjust two bundle capture luminous powers equal,
Two bundle capture optical registrations injecting are used for capture in sample cell 400 and rotating birefringence penetrates particles of material.
Described illuminator 300 is made up of LED/light source 301 and lens 302, and its Main Function is to provide for sample cell 400
Illumination.Sample cell 400 is the glass container being mounted with birefringence particulate 401.
Described speed measuring system 500 is by object lens 501, double-colored phase optical filtering 502, ccd image sensor 503, λ/4 wave plate
504, polarizing beam splitter mirror 505, lens 506, photodetector 507, lens 508 and photodetector 509 form.Birefringent material
The scattered light of particulate and illuminating bundle are divided into two bundles by double-colored phase optical filtering 502 after object lens 501 collection, wherein a branch of for illumination
Light, its transmission direction is constant, is input in ccd image sensor 503 to facilitate capture and the rotation to birefringent material particulate
Process is observed, another restraint as scattered light, its transmission direction injects λ/4 wave plate 504 after partially turning 90 degrees, scattering envelope contains left-handed
And right-circularly polarized light, when birefringent material particulate 401 rotates, the left-handed and right-circularly polarized light that scattered light is comprised
Shared ratio also changes.It is orthogonal that left-handed and right-circularly polarized light is changed into polarization direction after λ/4 wave plate 504 respectively
Linearly polarized light, inject polarizing beam splitter mirror 505, polarizing beam splitter mirror 505 by mutually perpendicular for polarization direction linearly polarized light separately, its
In a branch of linearly polarized light direction of propagation constant, be input to after lens 508 in photodetector 509, another bunch polarised light
The direction of propagation is input in photodetector 507 after partially turning 90 degrees after lens 506, and photodetector 507,509 detects
The ratio of light intensity signal can represent left-handed in scattered light and right-circularly polarized light light intensity ratio, by left-handed and right-circularly polarized light institute
The generating period change with the rotation of birefringent material particulate of accounting example, the period of change of the ratio of described light intensity signal is double
The rotation period of refractive material particulate, the rotating speed of birefringent material particulate is exactly the inverse of its rotation period.
Claims (3)
1. a kind of based on dual-beam ligh trap realize photic rotation device it is characterised in that：This device is by two sets of light beam regulation systems
System, sample cell, illuminator and five parts of speed measuring system form, and wherein two sets beam adjustment system produce power respectively
The different left-handed and right-circularly polarized light of identical, wavelength, described two bundle circularly polarized lights input and are aligned from transparent sample pond both sides,
Penetrate particles of material for capture and rotating birefringence, utilize illuminator to provide illumination below sample cell, and above sample cell
Observed and measured the rotary speed of birefringent material particulate using speed measuring system；
Wherein beam adjustment system is made up of laser instrument, λ/2 wave plate, polarizing beam splitter mirror and λ/4 wave plate, laser emitting continuous
Laser forms linearly polarized light after λ/2 wave plate and polarizing beam splitter mirror, described linearly polarized light through λ/4 wave plate, about adjusting
The optical axis of λ/4 wave plate described in two sets of beam adjustment system make its angle with described linearly polarized light polarization direction be respectively ±
45 °, so that shoot laser is changed into left-handed and right-circularly polarized light respectively, described left-handed and right-circularly polarized light are input to sample
As capture laser in pond, birefringent material particulate is captured and is rotated control；
Sample cell is the transparent glass container being mounted with birefringent material particulate, and illuminator is by LED/light source and focus lens group
Become, positioned at the lower section of sample cell, its Main Function is to provide illumination for sample cell；
Speed measuring system is by object lens, double-colored phase optical filtering, ccd image sensor, λ/4 wave plate, polarizing beam splitter mirror, two lens
Form with two photodetectors, collect capture light through birefringent material Particle Scattering using the object lens above sample cell
The scattered beam being formed afterwards and the illuminating bundle after illuminator passes through sample cell, and will be scattered using double-colored phase optical filtering
Separately, wherein illuminating bundle is input in the ccd image sensor above object lens so as to two-fold for light beam and illuminating bundle
Penetrate the capture of particles of material and rotary course is observed, scattering envelope contains left-handed and right-circularly polarized light, and scattered light is through double-colored
After phase optical filtering, the direction of propagation turn 90 degrees partially, through λ/4 wave plate, left-handed and right-circularly polarized light is changed into two bundle polarization direction phases
Mutually vertical linearly polarized light, recycles polarizing beam splitter mirror by the two orthogonal linearly polarized lights in bundle polarization direction separately, is changed into inclined
Shake that direction is constant but the mutually perpendicular two bunch polarised lights in the direction of propagation, every bunch polarised light, all after lens focus, is used respectively
Photodetector is detected, and the light intensity signal that two photodetectors are detected is divided by, and the period of change of gained signal is just
It is the rotation period of birefringent material particulate, the rotating speed of birefringent material particulate is exactly the inverse of its rotation period.
2. a kind of as claimed in claim 1 based on dual-beam ligh trap realize photic rotation device it is characterised in that：Two sets of light beams
The wavelength of regulating system output two bundle capture laser is all near infrared band.
3. a kind of the photic method rotating and measuring its rotating speed is realized using device as claimed in claim 1 it is characterised in that should
The comprising the following steps that of method：
Step one, assemble and as claimed in claim 1 the device of photic rotation is realized based on dual-beam ligh trap and adjust left and right two
Set beam adjustment system is so that two sets of beam adjustment system left-handed and dextrorotation circles that emergent power is identical, wavelength is different respectively are inclined
Shake light；
Step 2, two bundle circularly polarized lights are input in sample cell and are aligned, capture rotating birefringence penetrates particulate, and by light beam
Ccd image sensor in illuminator and speed measuring system is observed；
Step 3, using speed measuring system measure particulate rotary speed：The change of the ratio of two photodetector light intensity signals
The change cycle is the rotation period of birefringent material particulate, and the rotating speed of birefringent material particulate is exactly the inverse of its rotation period；
Step 4, the light intensity changing two bundle capture light using two sets of beam adjustment system and polarization state, thus change particulate
Position and rotary speed, and the situation of change of the rotary speed of particulate is observed using speed measuring system.
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| CN105469847A (en) * | 2015-11-29 | 2016-04-06 | 中国人民解放军国防科学技术大学 | Device for realizing light-induced track rotation based on double-light beam misalignment method and method |
| CN106908945B (en) * | 2016-05-05 | 2019-10-08 | 中国计量大学 | A kind of dual-beam optical tweezer based on optical modulator |
| CN106238928B (en) * | 2016-07-28 | 2017-09-29 | 孝感市森茂激光数控设备有限公司 | Laser probe is coaxial above and below laser cutting machine, elevating adjusting device and method |
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| CN106653136B (en) * | 2016-11-02 | 2017-12-29 | 中国人民解放军国防科学技术大学 | The method for demarcating particles position detector in dual-beam optical trap system |
| CN107607047B (en) * | 2017-09-14 | 2019-08-06 | 中国人民解放军国防科技大学 | Method for correcting misalignment of axis of image sensor in double-beam optical trap |
| CN108801602B (en) * | 2018-06-20 | 2020-04-17 | 中国人民解放军国防科技大学 | Device and method for reducing low-frequency noise in dual-beam optical trap system |
| CN110672465A (en) * | 2019-10-23 | 2020-01-10 | 中国人民解放军国防科技大学 | Device and method for measuring viscosity coefficient of micro-area space liquid by utilizing photoinduced orbital rotation technology |
| CN111061064B (en) * | 2019-12-30 | 2020-12-15 | 浙江大学 | Double-beam optical trap beam auxiliary alignment device and method |
| CN111816344B (en) * | 2020-07-01 | 2022-10-25 | 浙江大学 | Device for simultaneously manipulating low-refractive-index particles in multiple Rayleigh regions and achieving high capture efficiency |
| CN111899907B (en) * | 2020-08-10 | 2022-12-27 | 上海大学 | Conveying device |
| CN112730334B (en) * | 2020-12-23 | 2024-03-22 | 之江实验室 | Nanoparticle identification device and method based on electric dipole rotation scattered light detection |
| CN113502223A (en) * | 2021-07-12 | 2021-10-15 | 桂林电子科技大学 | Active optical control method and device for living body single cell rotation angle |
| CN113533177B (en) * | 2021-07-14 | 2022-05-20 | 清华大学 | Method for high-speed operation and detection of living cells |
| CN113804414B (en) * | 2021-09-18 | 2024-05-24 | 中国人民解放军国防科技大学 | Device and method for precisely aligning light beams of double-beam optical trap |
| CN117253644B (en) * | 2023-11-20 | 2024-02-20 | 之江实验室 | Double-beam vacuum optical tweezers system for researching photoinduction coupling interaction |
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| US7961318B2 (en) * | 2006-06-28 | 2011-06-14 | President And Fellows Of Harvard College | Circular birefringence refractometer: method and apparatus for measuring optical activity |
| CN101650166B (en) * | 2008-08-15 | 2012-04-11 | 上海理工大学 | Laser interference system used for measuring micro roll angle |
| CN103226248A (en) * | 2013-05-21 | 2013-07-31 | 上海理工大学 | Method for controlling focus rotation by utilizing orthogonal polarized beam |
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