CN111816344B - Device for simultaneously manipulating low-refractive-index particles in multiple Rayleigh regions and achieving high capture efficiency - Google Patents
Device for simultaneously manipulating low-refractive-index particles in multiple Rayleigh regions and achieving high capture efficiency Download PDFInfo
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- CN111816344B CN111816344B CN202010627958.9A CN202010627958A CN111816344B CN 111816344 B CN111816344 B CN 111816344B CN 202010627958 A CN202010627958 A CN 202010627958A CN 111816344 B CN111816344 B CN 111816344B
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Abstract
The invention discloses a device for simultaneously manipulating low-refractive-index particles in multiple Rayleigh regions and having high capture efficiency. Light beams emitted by the laser enter the transmission-type spatial light modulator after being expanded by the beam expander group, are incident on the bicolor beam splitter through the well position adjusting mirror, are reflected to the high-focusing objective lens and then output focused light, and the focused light is incident into the sample chamber to form a light trap and irradiates a sample in the sample chamber; a plurality of particles are arranged in the sample chamber, the illumination of the illuminating lamp penetrates through the sample chamber to irradiate the focus of the high-focus objective lens, and scattered light generated by the sample in the sample chamber irradiated by the focused light returns to form an image on the target surface of the CCD detector. The invention controls the transmission-type spatial light modulator to generate sine-modulated Gaussian beams of the multi-position light trap to obtain new light intensity distribution, stably captures particles with high refractive index and low refractive index in the range of a focus area, can simultaneously realize independent control on multiple particles, and improves the gradient force of the light trap by selecting the sine modulation coefficient g, thereby improving the capture efficiency.
Description
Technical Field
The invention relates to a novel optical tweezers capturing device belonging to the technical field of optical capturing and far-field control particles, provides a device capable of simultaneously and independently capturing particles with low refractive index and improving capturing efficiency, can be applied to the fields of cell microbiology and nanoparticle assembly, and belongs to the field of optical tweezers application.
Background
The purpose of creating an optical tweezers device is to create a high gradient optical field that can capture particles and then transport the particles by relative motion between the particles and the environment in which the light is located. Scientists have begun to generate great interest in the wide application of optical trapping technology in the field of micro-nano scale potential excellence and cell biology. The trapping properties of a light beam are related to its optical field. The optical tweezers field used in general is gaussian distributed, the cross section of the light beam is solid, and the intensity of the cross section is gaussian distributed gradually decreasing from the center to the edge. The Gaussian beam cannot capture particles with high refractive index and low refractive index at the same time, and only one or two particles can be captured at one time due to the fact that only one beam focusing center is provided.
The novel optical tweezers provided by the invention are provided with four independent Gaussian-distributed light beam focusing centers, and can capture a plurality of particles with high refractive indexes and low refractive indexes at the same time. The Gaussian beam light intensity has spatial three-dimensional symmetrical distribution, and is a typical light source with a gradient light field.
The laser beam focused by the lens exerts a force on the particles near the focal point thereof. The force of the particles in the optical trap is the result of the interaction between the laser and the particles, when the particles in the optical trap are nanoparticles in a Rayleigh region, i.e. the radius of the particles is far less than the wavelength, the nanoparticles are approximated to a dipole according to Rayleigh approximation, are subjected to a gradient force action in a non-uniform electromagnetic field, and are also subjected to a scattering force on the nanoparticles according to a scattering cross section. The scattering force pushes the particles away along the propagation direction of the light; the gradient force pushes the particle along the gradient direction of the light field to the position where the light field gradient is strongest. Because the particles are small, the gravity and the buoyancy are weak, but the particles are also collided by solution molecules, so that the brownian motion of the particles is severe, and the light trap is difficult to bind the particles to influence the capture of the particles. The necessary condition for optical tweezers to be formed is that the gradient force generated by the laser is greater than the scattering and brownian forces, and therefore the particles can be trapped near the center of the focused spot. The larger the gradient force generated by the laser, the deeper the potential well formed, and the more beneficial it is for trapping particles.
The multi-position optical trap refers to optical tweezers capable of forming a plurality of optical traps at one time and simultaneously capturing and manipulating a plurality of particles. Compared with single-beam optical tweezers which can capture only one particle at a time, the multi-beam optical traps can not only simultaneously generate a plurality of optical traps, but also control the arrangement positions of the optical traps in real time, and greatly improve the experimental efficiency. The multi-position optical trap generation method comprises a multi-beam interference method, a time division multiplexing method and spatial modulation optical tweezers. The multi-beam interference method can only generate a symmetrical structure, but can only realize two-dimensional capture, and the axial scattering force of the method needs to be overcome by other factors. Time division multiplexing can also produce multiple optical traps, but such multiple traps are a time-combining effect. The position of the laser can be changed very quickly in a short time by using an acousto-optic modulator or a scanning galvanometer, so that particles at different positions can experience an optical potential well in a time-averaging sense. But neither system based on these two scanning devices can produce a three-dimensional array of optical traps. The spatial modulation optical tweezers can modulate the amplitude or the phase of a light beam, can realize the manipulation modes of dynamic capture, optical transport, photoinduced stretching and the like of a three-dimensional space of a plurality of particles, and greatly expands the functionality and the application field of optical micro manipulation. The existing spatial modulation optical tweezers are not provided with a formula which is determined that a plurality of independent light intensity distribution centers exist on the same plane, so that the practical use is difficult.
Disclosure of Invention
The invention aims to overcome the problem that the existing single-beam optical tweezers technology cannot simultaneously and independently capture a plurality of particles with different low refractive indexes on the same plane, provides a device and a processing mode for simultaneously manipulating the particles with low refractive indexes in a Rayleigh region and improving the capturing efficiency, and can realize the capturing of the plurality of particles and simultaneously manipulate each particle by modulating the amplitude or the phase of a light beam through a spatial light modulator.
The technical scheme of the invention is as follows:
the invention comprises a laser, a beam expanding lens group, a transmission type spatial light modulator, a graphic controller, a well position adjusting lens, a bicolor beam splitter, a high-focusing objective lens, a sample chamber, an illuminating lamp and a CCD detector; the optical tweezers comprise two parts, one part is that light beams emitted by a laser enter a transmission type spatial light modulator after being expanded by a beam expanding lens group, the control end of the transmission type spatial light modulator is electrically connected with a graphic controller and is loaded and controlled by the graphic controller, light emitted from the transmission type spatial light modulator enters a bicolor beam splitter through a trap position adjusting mirror to be reflected, the light is reflected to a high-focusing objective through the bicolor beam splitter to output focusing light, and the focusing light enters a sample chamber to form an optical trap and irradiates a sample in the sample chamber; the illuminating lamp and the high-focusing objective lens are respectively arranged at two symmetrical sides of the sample chamber, the other path is an imaging light path, a plurality of particles are arranged in the sample chamber, the focus of the high-focusing objective lens is positioned below the sample chamber, the illuminating lamp illuminates the focus of the high-focusing objective lens after penetrating through the sample chamber, the particles are captured by a light trap at the focus of the high-focusing objective lens after falling from the sample chamber, scattered light generated by a sample in the sample chamber after being illuminated by the focused light is collected and returned by the high-focusing objective lens, the scattered light penetrates through the high-focusing objective lens and is transmitted by the dichroic beam splitter to be imaged on a target surface of the CCD detector, the CCD detector is connected with a computer, and the CCD detector transmits acquired image signals to the computer.
The invention adopts the graphic controller to carry out sine factor phase information modulation on the transmission type space light, thereby obtaining the required light beam to realize the manipulation of a plurality of Rayleigh region low refractive index particles and high capture efficiency, and improving the gradient force of the light trap through the change of the sine modulation coefficient.
The illuminating lamp, the sample chamber, the high-focus objective lens, the bicolor beam splitter and the CCD detector are sequentially arranged from top to bottom.
The spatial light modulator is a transmission-type spatial light modulator, a preset phase information graph is loaded on the transmission-type spatial light modulator through a graphic controller to realize sinusoidal phase modulation of a light beam, the light beam reflected and emitted by a two-color beam splitter is taken as an incident Gaussian light beam, the incident Gaussian light beam is changed into a light beam with four light intensity distribution centers after being subjected to sinusoidal phase modulation through the transmission-type spatial light modulator and taken as a sinusoidal modulation Gaussian light beam, and each light intensity distribution center and the vicinity thereof are taken as a light trap, so that the four light intensity distribution centers respectively and correspondingly form four independent light traps;
the method comprises the following steps of establishing a three-dimensional Cartesian coordinate system by taking the optical axis direction of a transmission type spatial light modulator as the z-axis direction, wherein the x axis and the y axis are mutually vertical and are vertical to the z axis, and a preset phase information graph is formed by setting a pixel plane of the transmission type spatial light modulator according to a transmission coefficient T of sinusoidal phase modulation calculated and obtained by the following formula:
wherein g is a sinusoidal modulation coefficient, i represents an imaginary number, x and y represent coordinate positions of a single pixel in the transmissive spatial light modulator on the x axis and the y axis, e represents a natural constant, and w represents a natural constant 0 Is incident Gaussian lightBeam waist radius of the beam. By reducing the sinusoidal modulation factor g by one order of magnitude, the optical trap gradient force can be improved by three orders of magnitude.
In the invention, the sine modulation coefficient g is reduced by one order of magnitude, the gradient force of the optical trap can be improved by three orders of magnitude, and the corresponding capture efficiency is also improved, so that the capture efficiency is greatly improved by adjusting the sine modulation coefficient g.
The trap position adjusting lens moves along the optical axis to further change the distance between the trap position adjusting lens and the high-focus objective lens along the optical axis, and the divergence angle of the light beam incident to the high-focus objective lens is changed to change the focus position of the high-focus objective lens, so that the purpose of adjusting the trap position is achieved.
Specifically, the distance R from the light intensity distribution center to the intersection point between the optical axis and the pixel plane of the transmissive spatial light modulator is changed by adjusting the sinusoidal modulation coefficient g of the transmissive spatial light modulator, and the specific changing method formula is as follows:
wherein a new sinusoidal modulation factor g corresponds to a new distance R.
The numerical aperture of the high-focusing objective lens is not less than 0.85.
In the present invention, the high refractive index fine particles are fine particles having a refractive index higher than that of water, and the low refractive index fine particles are fine particles having a refractive index lower than that of water.
The method of the invention controls the transmission type spatial light modulator to generate sine-modulated Gaussian beams of the multi-position light trap, obtains new light intensity distribution for capturing particles after passing through the Coriolis transformation and the lens system, can stably capture the particles with high refractive index and low refractive index in the range of a focus area, and can simultaneously realize independent control of multiple particles. The invention can improve the gradient force of the optical trap by selecting the sinusoidal modulation coefficient g, thereby improving the capture efficiency.
In specific implementation, when the size of the nanoparticles manipulated by the optical tweezers is far smaller than the wavelength of the trapping laser, a plurality of nanoparticles can be trapped in the optical trap to be assembled, and the assembly mode is called as optical assembly. When the optical trap is closed, the nanoparticles are separated from each other due to factors such as mutual repulsion and brownian motion.
The invention has the beneficial effects that:
1. the invention has strong expansibility.
The environment of the particles proposed by the invention is an aqueous solution, and the refractive index of water is 1.33. For medium nano particles of various materials and sizes, the method can realize stable optical control, and only needs to simply adjust focusing conditions according to different conditions.
2. The invention has strong functionality. Unlike a conventional gaussian beam, there is only one focus center. Often only one high index particle is trapped in the center of focus. The method and the device for simultaneously manipulating the high-refractive-index particles and the low-refractive-index particles in the Rayleigh region and improving the capturing efficiency have four light intensity distribution centers, greatly improve the number of the captured particles, simultaneously improve the gradient force of two orders of magnitude and three orders of magnitude by reducing the sinusoidal modulation coefficient g, and realize the stable capturing of a plurality of high-refractive-index particles and low-refractive-index particles.
3. The method for generating the sine modulation light beam is simple to operate. The sine factor phase information modulation is carried out on the transmission type space light by adopting the graphic controller, so that the required light beam is obtained.
4. The invention can capture the particles with high refractive index and low refractive index by measuring and observing, shooting the capture condition of the particles by a CCD detector, and acquiring and processing the graphic information of the light-operated particles by a computer to obtain an accurate result.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings used in the embodiments or the prior art descriptions will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings without creative efforts.
FIG. 1 is a schematic view of the structure of the apparatus of the present invention.
Fig. 2 transversal gradient force diagram of a captured light field at sinusoidal modulation coefficient g =1 of the present invention.
Fig. 3 longitudinal gradient force diagram of captured light field at sinusoidal modulation factor g =1 of the present invention.
Fig. 4 is a scattering diagram of a captured light field at a sinusoidal modulation coefficient g =1 in accordance with the present invention.
Figure 5 is a graph of the change in longitudinal gradient force, scattering force, transverse gradient force and brownian force with trapped particle size for a sinusoidal modulation factor of g =1 according to the invention.
FIG. 6 is a graph of the lateral gradient force of the present invention as a function of the sinusoidal modulation factor g.
In the figure: the system comprises a laser 1, a beam expander 2, a beam expander set 3, a transmission type spatial light modulator 4, a graphic controller 5, a trap position adjusting mirror 6, a dichroic beam splitter 7, a high-focus objective lens 8, a sample chamber 9, a lighting lamp and a computer 10.
Detailed Description
To make the objects, technical solutions and advantages of the present application more clear, the present invention is further described below with reference to the accompanying drawings and examples, and embodiments of the present invention include, but are not limited to, the following examples. All other embodiments that can be obtained by a person skilled in the art without any inventive step based on the embodiments of the present application belong to the protection scope of the present application.
The invention is further described below with reference to the accompanying drawings.
As shown in fig. 1, the device for implementing the method includes a laser 1, a beam expander set 2, a transmissive spatial light modulator 3, a graphic controller 4, a well position adjusting mirror 5, a dichroic beam splitter 6, a high-focus objective lens 7, a sample chamber 8, an illuminating lamp 9 and a CCD detector 10; the illuminating lamp 9, the sample chamber 8, the high-focus objective lens 7, the dichroic beam splitter 6 and the CCD detector 10 are sequentially arranged from top to bottom. The optical tweezers comprise two parts, one part is that light beams emitted by a laser 1 are expanded by a beam expander group 2 and then enter a transmission-type spatial light modulator 3, the control end of the transmission-type spatial light modulator 3 is electrically connected with a graphic controller 4 and is controlled by the loading of the graphic controller 4, the light emitted from the transmission-type spatial light modulator 3 is incident on a bicolor beam splitter 6 through a well position adjusting mirror 5 to be reflected, the light is output after being reflected to a high-focus objective lens 7 through the bicolor beam splitter 6, and the focused light is incident into a sample chamber 8 to form an optical trap and irradiates a sample in the sample chamber 8; the illuminating lamp 9 and the high-focusing objective lens 7 are respectively arranged at two symmetrical sides of the sample chamber 8, the other path is an imaging light path, a plurality of particles are arranged in the sample chamber 8, the focus of the high-focusing objective lens 7 is positioned below the sample chamber 8, the illuminating lamp 9 illuminates the focus of the high-focusing objective lens 7 after penetrating through the sample chamber 8, the particles are captured by a light trap at the focus of the high-focusing objective lens 7 after falling from the sample chamber 8, scattered light generated by the sample in the sample chamber 8 after being illuminated by the focused light is collected and returned by the high-focusing objective lens 7, the scattered light penetrates through the high-focusing objective lens 7 and is transmitted by the dichroic beam splitter 6 to be imaged on a target surface of the CCD detector 10, the CCD detector 10 is connected with a computer, and the CCD detector 10 transmits the acquired image signal to the computer.
The invention adopts the graphic controller 4 to carry out sine factor phase information modulation on the transmission-type space light, thereby obtaining the required light beam to realize the manipulation of a plurality of Rayleigh region low refractive index particles and high capture efficiency, and improving the gradient force of the optical trap through the change of a sine modulation coefficient.
The spatial light modulator is a transmission-type spatial light modulator, a preset phase information graph is loaded on the transmission-type spatial light modulator 3 by a graphic controller 4 to realize sinusoidal phase modulation of a light beam, the light beam reflected and emitted by a two-color beam splitter 6 is taken as an incident Gaussian light beam, the incident Gaussian light beam is changed into a light beam with four light intensity distribution centers after being subjected to sinusoidal phase modulation by the transmission-type spatial light modulator 3 and taken as a sinusoidal modulation Gaussian light beam, and each light intensity distribution center and the vicinity thereof are taken as a light trap, so that the four light intensity distribution centers respectively and correspondingly form four independent light traps.
The method comprises the following steps of establishing a three-dimensional Cartesian coordinate system by taking the optical axis direction of a transmission type spatial light modulator 3 as the z-axis direction, wherein the x axis and the y axis are mutually vertical and are vertical to the z axis, and a preset phase information graph is formed by setting a pixel plane of the transmission type spatial light modulator 3 according to a transmission coefficient T obtained by calculating according to the following formula:
wherein g is a sinusoidal modulation coefficient, i represents an imaginary number, x and y represent coordinate positions of a single pixel in the transmissive spatial light modulator 3 on an x axis and a y axis, e represents a natural constant, and w represents a natural constant 0 The waist radius of the incident gaussian beam. By reducing the sinusoidal modulation factor g by one order of magnitude, the optical trap gradient force can be improved by three orders of magnitude.
In the invention, the sine modulation coefficient g is reduced by one order of magnitude, the gradient force of the optical trap can be improved by three orders of magnitude, and the corresponding capture efficiency is also improved, so that the capture efficiency is greatly improved by adjusting the sine modulation coefficient g.
The trap position adjusting mirror 5 moves along the optical axis to further change the distance between the trap position adjusting mirror and the high-focus objective lens 7 along the optical axis, and the divergence angle of the light beam incident to the high-focus objective lens 7 is changed to change the focus position of the high-focus objective lens 7, so that the purpose of adjusting the trap position is achieved.
Specifically, the distance R from the light intensity distribution center to the intersection point between the optical axis and the pixel plane of the transmissive spatial light modulator 3 is changed by adjusting the sinusoidal modulation coefficient g of the transmissive spatial light modulator 3, and the specific changing method formula is as follows:
wherein a new sinusoidal modulation factor g corresponds to a new distance R.
In the specific implementation of the present invention, the electric field distribution E of the sine-modulated gaussian beam passing through the liquid crystal spatial light modulator at the transmission distance z =0 (i.e. at the pixel plane of the liquid crystal spatial light modulator) is:
wherein E (x 1, y1, 0) represents the electric field of the pixel at the x1, y1 coordinate position in the liquid crystal type spatial light modulator 4, E 0 Is the initial electric field strength.
The light intensity focused at the optical trap is obtained from the electric field:
wherein n is m Is the refractive index of the environment in which the particles are located, c is the speed of light, ε 0 Is the dielectric constant in vacuum.
The following optical trapping forces were then calculated:
gradient force:
scattering force:
brown force:
wherein Cpr is a radiation pressure section coefficient, a is a particle radius, eta is a viscosity coefficient of an environment, kB is a Boltzmann constant, T is an environment temperature, nr is a relative refractive index, namely a refractive index of a particle to be captured is higher than a refractive index of the environment, and k represents a wave number of the laser.
A specific distribution of gradient forces, scattering forces, brownian forces in the radial direction and in the axial direction is thereby obtained. By comparing the gradient force and the scattering force, the magnitude of the brownian force and the two conditions for stable capture. And further, the invention is determined to be scientific and feasible by specific implementation of analysis processing.
The embodiment of the invention and the implementation process thereof are as follows:
hereinafter, taking the example that a sample stage is immersed in water, and air bubbles and polystyrene spheres with the radius of 20 nanometers are placed on the sample stage, the method provided by the patent can realize stable three-dimensional capture by combining with the attached drawings.
Air bubbles (refractive index 1) and polystyrene beads (refractive index 1.59) were immersed in water (refractive index 1.33), the focal length of the high focus objective lens was 5 mm, the incident optical power of the laser was 100 mw, and the wavelength of the 1064 nm infrared light generated optical power was sufficient to support stable optical manipulation.
The particles on the sample stage are separated from the sample stage under external vibration and fall into an optical field formed by the high-focusing objective lens, and when gradient force borne by the particles is balanced with three forces, namely particle gravity, scattering force and Brown force, the particles stably stay in the optical field and cannot continuously fall. The microparticle weight was four orders of magnitude less than the gradient force.
Fig. 2 shows the gradient force of the optical field in the transverse direction of the air bubble and polystyrene bead, respectively, and nr is the relative refractive index, i.e. the refractive index of the particle to be trapped is higher than that of water.
FIG. 3 shows the gradient force of the light field applied to the air bubbles and polystyrene beads in the longitudinal direction, respectively. Comparing fig. 2 and 3, we have found that the particles are subjected to a gradient force in the transverse direction which is greater than the longitudinal gradient force.
Fig. 4 shows the scattering force of the light field in the longitudinal direction of the air bubble and polystyrene bead, respectively, and nr is the relative refractive index, i.e. the refractive index of the particle to be trapped is higher than that of water. Comparing fig. 4 and fig. 3, it can be seen that the longitudinal gradient force is an order of magnitude greater than the longitudinal scattering force.
Fig. 5 shows the change of transverse gradient force, longitudinal scattering force and brownian force experienced by the particle as the radius of the particle is changed. It can be seen that at less than 5 nm, the brownian force of the particle is less than the gradient force of the particle.
Fig. 6 shows the distribution of the transverse gradient force as a function of the sinusoidal modulation factor g. The sinusoidal modulation coefficient g is reduced by one order of magnitude, and the gradient force of the optical trap can be improved by three orders of magnitude.
It can be seen from the above experiments that the gradient force generated by the laser in the technical scheme is greater than the scattering force and the brownian force to form the optical tweezers, so that the particles can be captured near the center of the focused light spot. The gradient force generated by the laser energy is larger, which is beneficial to capturing particles.
Claims (4)
1. A device for simultaneously manipulating a plurality of rayleigh regions of low refractive index particles with high trapping efficiency, comprising: the device comprises a laser (1), a beam expander set (2), a transmission type spatial light modulator (3), a graphic controller (4), a well position adjusting mirror (5), a bicolor beam splitter (6), a high-focus objective lens (7), a sample chamber (8), an illuminating lamp (9) and a CCD detector (10); light beams emitted by a laser (1) are expanded by a beam expander group (2) and then enter a transmission-type spatial light modulator (3), light emitted from the transmission-type spatial light modulator (3) is incident on a dichroic beam splitter (6) through a trap position adjusting mirror (5) to be reflected, the light is reflected to a high-focus objective lens (7) through the dichroic beam splitter (6) to be output as focused light, and the focused light is incident into a sample chamber (8) to form a light trap and irradiates a sample in the sample chamber (8); the illuminating lamp (9) and the high-focus objective lens (7) are respectively arranged at two symmetrical sides of the sample chamber (8), a plurality of particles are arranged in the sample chamber (8), the focus of the high-focus objective lens (7) is positioned below the sample chamber (8), the illuminating lamp (9) illuminates the focus of the high-focus objective lens (7) after penetrating through the sample chamber (8), scattered light generated by the sample in the sample chamber (8) after being illuminated by the focused light is collected and returned by the high-focus objective lens (7), and is transmitted by the dichroic beam splitter (6) to be imaged on the target surface of the CCD detector (10) after penetrating through the high-focus objective lens (7), and the CCD detector (10) is connected with a computer;
the preset phase information graph is loaded on the transmission type spatial light modulator (3) by the graph controller (4) to realize sinusoidal phase modulation of the light beam, the light beam reflected and emitted by the two-color beam splitter (6) is used as an incident Gaussian light beam, the incident Gaussian light beam is changed into a light beam with four light intensity distribution centers after being subjected to sinusoidal phase modulation by the transmission type spatial light modulator (3) and is used as a sinusoidal modulation Gaussian light beam, and each light intensity distribution center and the vicinity thereof are used as a light trap, so that the four light intensity distribution centers respectively and correspondingly form four independent light traps;
the method comprises the following steps of establishing a three-dimensional Cartesian coordinate system by taking the optical axis direction of a transmission type spatial light modulator (3) as the z-axis direction, wherein the x-axis and the y-axis are mutually vertical and are vertical to the z-axis, and a preset phase information graph is formed by setting a pixel plane of the transmission type spatial light modulator (3) by calculating and obtaining a transmission coefficient T of sine phase modulation according to the following formula:
wherein g is a sinusoidal modulation coefficient, i represents an imaginary number, x and y represent coordinate positions of a single pixel in the transmissive spatial light modulator (3) on an x axis and a y axis, e represents a natural constant, w 0 Is the beam waist radius of the incident gaussian beam.
2. The device of claim 1, wherein the device is capable of simultaneously manipulating a plurality of rayleigh regions with low refractive index particles and high trapping efficiency, and comprises: the illuminating lamp (9), the sample chamber (8), the high-focus objective lens (7), the dichroic beam splitter (6) and the CCD detector (10) are sequentially arranged from top to bottom.
3. The device of claim 1 for simultaneously manipulating multiple rayleigh regions low index particles with high capture efficiency, wherein: the trap position adjusting lens (5) moves along the optical axis to further change the distance between the trap position adjusting lens and the high-focus objective lens (7) along the optical axis, and the divergence angle of the light beam incident to the high-focus objective lens (7) is changed to change the focal position of the high-focus objective lens (7), so that the purpose of adjusting the trap position is achieved.
4. The device of claim 1 for simultaneously manipulating multiple rayleigh regions low index particles with high capture efficiency, wherein: the numerical aperture of the high-focusing objective lens (7) is not less than 0.85.
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