CN104886038A - Method for producing animal specimen - Google Patents
Method for producing animal specimen Download PDFInfo
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- CN104886038A CN104886038A CN201510307690.XA CN201510307690A CN104886038A CN 104886038 A CN104886038 A CN 104886038A CN 201510307690 A CN201510307690 A CN 201510307690A CN 104886038 A CN104886038 A CN 104886038A
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Abstract
The invention particularly relates to a method for producing an animal specimen. In order to reduce the production cost of the animal specimen, simplify production steps, reduce the production difficulty and reduce the damages to specimen producers and users, caused by substances including formalin, arsenic and the like which are used in an existing animal specimen production method, the animal specimen is produced by adopting a cyanoacrylate binding agent in a sealed manner based on the advantages of the cyanoacrylate binding agent of quick drying, strong binding property and the like; and the product is used for repairing crushed animal specimen limbs, sticking animal bones and imitating biological phenomena of the living nature. Compared with traditional animal stripping specimen production and animal immersing specimen production, the method has very great advantages in the aspects of production cost, operation steps, safety, and preservation and utilization of the specimen; and the method can relate to the field which cannot be reached by an existing specimen production method, namely the production of compound type specimens.
Description
Technical field
The present invention relates to a kind of method making zoological specimens, be specifically related to a kind of method that cyanoacrylate binding agent makes zoological specimens.
Background technology
Sample is the firsthand information of bioscience research, is the optimal material being engaged in practical teaching, and for student, sample is the best instrument cultivated observation ability, ability of pinpointing the problems and obtain new knowledge.Existing Slide processing, particularly zoological specimens preparation method, the noxious materials such as most employing formalin, phenol, arsenic carry out anticorrosion, and these medicaments all can produce injury to a certain degree to preparation of specimen person and sample user, and preparation method is complicated, operating process technical requirement is high, cost of manufacture is high, this all limits the scope of application and the practical value of sample.
Summary of the invention
For the problems referred to above, the invention provides a kind of method making zoological specimens, the method can improve holding time and the preservation effect of sample greatly, reduce the cost of preparation of specimen, simplify the step of preparation of specimen, eliminate in preparation of specimen process the injury using chemical reagent to preparation of specimen person and user, make common zoological specimens fan oneself can produce zoological specimens, enriched the teaching material of teacher.
For solving the problem, the present invention is achieved through the following technical solutions:
Design a kind of method making zoological specimens, comprise immersion, dehydration, sealing successively and preserve step, adopt cyanoacrylate binding agent to seal.
For the animal that cranial cavity is larger, first use syringe to cranium intracavitary administration cyanoacrylate binding agent, consumption is about 1/4th of cranial capacity, by cyanoacrylate binding agent, animal body surface is coated onto uniformly for the animal without fur, thickness 2 ~ 3 millimeters, joint for insect can increase usage amount, and is undertaken bonding by the limbs cyanoacrylate binding agent be damaged.For the animal having fur, first fur and health are peeled off, after dewatering completely, first animal body surface and inner chamber is evenly applied to cyanoacrylate binding agent, and it is inner cavity filled with cotton-wool, with cyanoacrylate binding agent cotton-wool soaked into completely and glues and conciliate plane place, then with cyanoacrylate binding agent by animal skin and animal body sticky with to together with.
For
onstate the method making zoological specimens, described soaking step is as follows: described soaking step is as follows: get animal remains, retain its complete corpse trunk, and thrust the pin hole of some (effect of pin hole is: be more conducive to alcohol and enter in spoil in alcohol-pickled process at its back, the drying of more favourable corpse when drying, more be conducive to binding agent when using the sealing of cyanoacrylate binding agent and immerse corpse inside, the quantity of pin hole is according to animal body size, be as the criterion not affect outward appearance), then 18 ~ 30 hours are immersed in alcohol, preferred volume concentration is 95% alcohol water blend
,described dehydration is as follows: take out spoil after soaking, and arrange corpse form, and fix its form, placement dries in the sun, described preservation step is as follows: after spoil dewaters completely, adopts cyanoacrylate binding agent to seal it, described preservation step is as follows: naturally to be dried or air-dry by the spoil after sealing, preserves.
For
onstate the method making zoological specimens, described soaking step is as follows: be soaked in by animal skeleton in alcohol, takes out after 30 ~ 40 hours, changes alcohol, again soaks 42 ~ 50 hours; Described dehydration is as follows: dry under the animal skeleton taken out after soaking is placed in sunlight; Described sealing step is as follows: after dewatering, animal skeleton is according to its physiological structure, arranges form, adopts cyanoacrylate binding agent animal skeleton to seal; Described preservation step is as follows: under the animal skeleton after sealing is placed in sunlight, make adhesive cures, preserve.
For
onstate the method making zoological specimens, described cyanoacrylate binding agent is a-cyanoacrylate class binding agent.
For
onstate the method making zoological specimens, the active ingredient of described cyanoacrylate binding agent is ECA.
For
onstate the method making zoological specimens, described cyanoacrylate binding agent is 101 glue or 502 glue, all commercially available, also can prepare voluntarily.
the present invention has following actively useful effect:
Sudden reaction can be there is in the present invention's cyanoacrylate binding agent used under the catalysis of minor amount of water in atmosphere, rapid solidification and adherend is cemented, inventor passes through lot of experiments, adopt a kind of mode being different from conventional method completely to make zoological specimens, to make up the various defects existing for prior art.The method can improve holding time and the preservation effect of sample greatly, reduce the cost of preparation of specimen, simplify the step of preparation of specimen, eliminate in preparation of specimen process the injury using existing chemical reagent to preparation of specimen person and user, make common zoological specimens fan oneself can produce zoological specimens, enrich the teaching material of teacher.
Embodiment
Below in conjunction with specific embodiment, the specific embodiment of the present invention is described, but following examples are only used to describe the present invention in detail, and limit the scope of the invention never in any form.Raw material involved in following embodiment are commercially available if no special instructions, and involved detection method is conventional method if no special instructions.
embodiment 1
Make a method for dinodon rufozonatum snake sample, comprise the following steps:
(1) soak: get dinodon rufozonatum snake remains, retain snake bone, skin and whole muscle, utilize and separate plane pin thrusts some pin hole at its back, the size of pin hole is good not affect outward appearance, and being then immersed in volumetric concentration is in 95% alcohol 24 hours;
(2) dehydration: take out dinodon rufozonatum snake corpse after soaking, arranges corpse form, and also fixes its form with solution plane pin, and placement dries in the sun;
(3) seal: after dinodon rufozonatum snake corpse dewaters completely, utilize 502 glue to seal.
(4) preserve: the snake after sealing is placed in ventilation and dries, can preserve.
The making of the individual sample of dinodon rufozonatum snake saves the skin of snake, bone and whole muscle, improves the firmness degree of snake sample individuality simultaneously, substantially increases the holding time of sample, decrease the destruction of chemical reagent to animal body.
Relatively can find by different specimens preparation method, utilize cyanoacrylate binding agent to make zoological specimens and no matter in cost of manufacture, operating procedure, safety, preservation and use, all there is very large advantage, in table 1 than making such as the stripping of traditional animal sample, animal immersed specimen etc.
Table 1 technology contrast table (poisonless snakes for 1.5 meters)
101,502 glue all belong to the glue of cyanoacrylate ester class, under the catalysis of minor amount of water in atmosphere, sudden reaction can occur, rapid solidification and being cemented by adherend.Because be colourless transparent liquid before its reaction, there is stronger permeability, stable in properties after reaction solidification, nontoxic, so become the good reagent of sealing zoological specimens.
embodiment 2
The preparation method of a kind of compound sample (imitating masked civet putrefaction process), comprises the following steps:
(1) soak: masked civet bone is soaked in alcohol, take out after 36 hours, change alcohol, brewed 48 hours again; Yellow mealworm after complete death, click beetle, earwig are placed in alcohol 12 hours;
(2) dewater: under the masked civet bone after immersion and yellow mealworm, click beetle, earwig etc. are placed in sunlight, dry dehydration; Dry ground is dewatered in the sun completely, grind up powder;
(3) seal: by the bone of masked civet according to its physiological structure, arrange form in cartridge bottom, and arrange the thickness of dry ground according to the form that sample is placed; Respectively the insect that the masked civet bone arranged and participation rot is sealed with 101 glue, and inlay with the insect such as yellow mealworm, click beetle, earwig participating in corpse putrefaction process;
(4) preserve: make glue curing in the sun, and make regular check in the following period of time in future, observe whether there is native seepage, seal with 101 if any native seepage, as without native seepage, namely complete.
Imitate the making of masked civet putrefaction process sample, be intended to certain biological phenomenon in natural imitation circle, may be used for after making in the middle of practical teaching process, because draw materials conveniently, make simple, there is very strong practicality.Illustrate and utilize cyanoacrylate binding agent not only can make single individual sample, and can certain phenomenon relevant with biology of natural imitation circle, set foot in the field that Slide processing till now cannot relate to, i.e. the making of compound sample.
For dinodon rufozonatum snake, now use the preparation method of skinned-specimen as follows:
(1)material processed
Surveying record is carried out to the size at each position of snake body, plots profile sketch, indicate body colour when living and common form, the foundation as measurement data and when inserting prosthese;
(2)peeling fleshing
Snakehead base portion is tied up a soft rope, hang on his thing, cutting snakeskin about 5 ~ 10cm(otch length from head veutro 3cm place with scalpel, determine according to the size of snake body), then, with cutter separating cut place skin and flesh, and cut off backbone with bone shears from upper cut, a body is separated.Carefully skin is turned up again, drop-down, while make skin and flesh be separated with cutter.Now when carpel is torn and scale comes off, and cause sample mistake shape.Shell the intestines to noting retaining certain length during anus hole, in case skin is torn, shell to tail end always.From rope, skin is taken off again, and open rope and short the rod of binding head, then peel head skin to rhynchodaenm edge, reject the muscle in remaining backbone and head, dig up eyeball and brain.If poisonous snake, cut anti-poison fang endogenous toxic material liquid and hurt sb.'s feelings, application gauze encases head, slowly peels off head skin.In addition for the female snake having ovum in abdomen, when peeling is to belly, ovum hand should be extruded carefully from bottom to top one by one, otherwise subcutaneous stripping is difficult, and causes skin and tear, scale comes off;
(3)the making of prosthese and filling
Select the iron wire of suitable thickness according to the size of snake body, get the iron wire being longer than snake body 10cm length, be wrapped with bamboo silk or cotton above, and fixing with fine rule binding, and thickness length is equal, thin-to-thick with measured snake body, and change is evenly.Then intracutaneous is loaded.Before filling out prosthese, first preservative treatment, to snakeskin, skull and spinal bone being smeared preservative (arsenic cream or other anticorrosion medicines), the cotton scribbling preservative is loaded in cranial cavity, head all rejecting muscle place, all loads a certain amount of cotton, and is turned back by head skin cylinder.
By ready-made prosthese from tail is most advanced and sophisticated, varus snakeskin cylinder careful on one side, while load prosthese until incision, the prosthese other end is longer than the iron wire of snake body, be fixed on vertebrae and skull, and insert appropriate cotton with tweezers, dissecting needle to vertebra surrounding carefully, make a body thickness suitably and coincide with prosthese.Then use wax wire (look consistent with snake abdomen fish) to sew up the incision carefully, degree of tightness is moderate, and interior thing does not leak.Then load onto ocular prosthesis according to the size of eye, and according to exact shape (body the is slightly triangular in shape) shaping of snake, its thickness is changed evenly.
Above embodiment is described in detail the present invention, but, person of ordinary skill in the field can understand, under the prerequisite not departing from present inventive concept, each design parameter in above-described embodiment can also be changed, form multiple specific embodiment, be common excursion of the present invention, describe in detail no longer one by one at this.
Claims (6)
1. make a method for zoological specimens, comprise immersion, dehydration, sealing successively and preserve step, it is characterized in that: adopt cyanoacrylate binding agent to seal.
2. make the method for zoological specimens according to claim 1, it is characterized in that: described soaking step is as follows: get animal remains, retain its complete corpse trunk, and thrust the pin hole of some at its back, to be then immersed in alcohol 18 ~ 30 hours
;described dehydration is as follows: take out spoil after soaking, and arrange corpse form, and fix its form, placement dries in the sun; Described preservation step is as follows: after spoil dewaters completely, adopts cyanoacrylate binding agent to seal it; Described preservation step is as follows: naturally to be dried or air-dry by the spoil after sealing, preserves.
3. make the method for zoological specimens according to claim 1, it is characterized in that: described soaking step is as follows: animal skeleton is soaked in alcohol, take out after 30 ~ 40 hours, change alcohol, again soak 42 ~ 50 hours; Described dehydration is as follows: dry under the animal skeleton taken out after soaking is placed in sunlight; Described sealing step is as follows: after dewatering, animal skeleton is according to its physiological structure, arranges form, adopts cyanoacrylate binding agent animal skeleton to seal; Described preservation step is as follows: under the animal skeleton after sealing is placed in sunlight, make adhesive cures, preserve.
4. make the method for zoological specimens according to claim 1, it is characterized in that, described cyanoacrylate binding agent is a-cyanoacrylate class binding agent.
5. make the method for zoological specimens according to claim 4, it is characterized in that, the active ingredient of described cyanoacrylate binding agent is ECA.
6. make the method for zoological specimens according to claim 4, it is characterized in that, described cyanoacrylate binding agent is 101 glue or 502 glue.
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| CN201510307690.XA CN104886038B (en) | 2015-06-08 | 2015-06-08 | The method for making zoological specimens |
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| CN201510307690.XA CN104886038B (en) | 2015-06-08 | 2015-06-08 | The method for making zoological specimens |
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| CN104886038B CN104886038B (en) | 2017-06-16 |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105632313A (en) * | 2016-04-01 | 2016-06-01 | 昆山周庄鸿峰生命奥秘博物馆有限公司 | Preparation method of lizard specimen |
| CN106070183A (en) * | 2016-08-25 | 2016-11-09 | 卞陆杰 | The manufacture method of zoological specimens |
| CN106257567A (en) * | 2016-03-11 | 2016-12-28 | 宁夏大学 | Lumbricus section and manufacture method and application thereof for microsurgery training |
| CN106689114A (en) * | 2017-01-05 | 2017-05-24 | 卞陆杰 | Method for making stuffed animal specimen of hairless vertebrate and injector adopted by same |
| CN107183002A (en) * | 2017-05-10 | 2017-09-22 | 广西柳州中嘉知识产权服务有限公司 | Butterfly specimen preparation method |
| CN109094980A (en) * | 2018-10-09 | 2018-12-28 | 青岛农业大学 | A kind of porous glue bottle of Teflon and its application method |
| CN110476949A (en) * | 2019-08-22 | 2019-11-22 | 云南省热带作物科学研究所 | A kind of Slide processing of Africangiant snail |
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| CN103430938A (en) * | 2013-08-30 | 2013-12-11 | 王荣林 | Preparation method of frog cardiovascular and saccus lymphaticus lumen colored specimen |
| CN103525119A (en) * | 2013-10-24 | 2014-01-22 | 江苏省家禽科学研究所 | Cartilage staining solution, bone staining method and method for preparing embryonic bone specimen by using bone staining method |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106257567A (en) * | 2016-03-11 | 2016-12-28 | 宁夏大学 | Lumbricus section and manufacture method and application thereof for microsurgery training |
| CN106257567B (en) * | 2016-03-11 | 2020-04-10 | 宁夏大学 | Earthworm section for microsurgery training, and manufacturing method and application thereof |
| CN105632313A (en) * | 2016-04-01 | 2016-06-01 | 昆山周庄鸿峰生命奥秘博物馆有限公司 | Preparation method of lizard specimen |
| CN106070183A (en) * | 2016-08-25 | 2016-11-09 | 卞陆杰 | The manufacture method of zoological specimens |
| CN106689114A (en) * | 2017-01-05 | 2017-05-24 | 卞陆杰 | Method for making stuffed animal specimen of hairless vertebrate and injector adopted by same |
| CN107183002A (en) * | 2017-05-10 | 2017-09-22 | 广西柳州中嘉知识产权服务有限公司 | Butterfly specimen preparation method |
| CN109094980A (en) * | 2018-10-09 | 2018-12-28 | 青岛农业大学 | A kind of porous glue bottle of Teflon and its application method |
| CN110476949A (en) * | 2019-08-22 | 2019-11-22 | 云南省热带作物科学研究所 | A kind of Slide processing of Africangiant snail |
| CN110476949B (en) * | 2019-08-22 | 2021-09-14 | 云南省热带作物科学研究所 | Preparation method of specimen of African big snail |
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| CN104886038B (en) | 2017-06-16 |
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