CN104885974B - A kind of extra large crabbing waterless keep-alive method - Google Patents
A kind of extra large crabbing waterless keep-alive method Download PDFInfo
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- CN104885974B CN104885974B CN201410824329.XA CN201410824329A CN104885974B CN 104885974 B CN104885974 B CN 104885974B CN 201410824329 A CN201410824329 A CN 201410824329A CN 104885974 B CN104885974 B CN 104885974B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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Abstract
The invention discloses a kind of extra large crabbing waterless keep-alive method, comprise the following steps:(1)Store up:After big chela plastic ties or the rubber band binding of the work sea crab of capture, it is placed in the seawater added with live keeping agent and temporarily supports 1 ~ 2 day;(2)It is classified anesthesia by freezing:Work sea crab after first storing up is placed in 1 ~ 2min of immersion in 7 ~ 8 DEG C of seawater, places into 1 ~ 2min of immersion in 1 ~ 2 DEG C of seawater;(3)Packaging:First in packing case bottom layer overlay oxygenation agent, then a waterishlogged sponge is put on oxygenation agent, last layer work sea crab is arranged on sponge, a waterishlogged sponge is put again, arrange one layer of sea crab living, put and fill two layers of swimming crab in a waterishlogged sponge, packing box altogether, packing case is simultaneously placed in preservation under 2 ~ 5 DEG C of environment by last joint sealing.The present invention is easy to operate, with low cost, can effectively improve extra large crabbing survival rate and time-to-live, and extra large crabbing long range, long-time On Transportaion of Live are realized with anhydrous packaged form, and remarkable in economical benefits is adapted to popularization and application.
Description
Technical field
The present invention relates to aquatic products circulation technology field, more particularly, to a kind of extra large crabbing waterless keep-alive method.
Background technology
Extra large crabbing, it is nutritious due to unique flavor such as swimming crab, mud crab, Sea crab, it is deep by consumer's
Welcome, the huge market demand.But extra large crabbing after capture due to environmental change, the time-to-live is short, easily dead rotten, mouthfeel
It is deteriorated with local flavor, it is edible to be substantially reduced with economic worth.Therefore, extend the time-to-live of extra large crabbing, just can significantly improve sea
The edible and economic worth of crabbing.
A kind of three wart is disclosed in Authorization Notice No. CN102084836B, authorized announcement date 2012.12.19 Chinese patent
Swimming crab comprises the following steps from water preserving active method:(1) purified in Portunus trituberculatus Miers seawater;Portunus trituberculatus Miers is put again
Enter in 2 ~ 5 DEG C of seawater and soak 3 ~ 5 minutes, be at containing 1 ‰ stable type in narcosis, 2 ~ 5 DEG C described of seawater
Vitamin C;(2) moisturizing material is put in 2 ~ 5 DEG C of seawater containing 1 ‰ stable vitamin C and is dipped to saturation;(3) exist
Oxygenation agent is first put into cavity in the middle of packing box, one layer of moisturizing material is then laid above oxygenation agent;In moisturizing material
Portunus trituberculatus Miers is placed in top, places one layer of moisturizing material i.e. second layer moisturizing material again above Portunus trituberculatus Miers, has placed
Cheng Hou, packing box lid is covered.The preservation method has the following disadvantages:Directly swimming crab is placed in 2 ~ 5 DEG C of seawater, temperature
Difference is too big, and crab does not adapt to unexpected environment, can produce disconnected foot or even dead, reduce survival rate;Added in seawater ascorbic
Vitamin C can only alleviate the stress reaction of crab body, between crab picks up from water and packed, easily cause crab hypoxia death;Increase
Oxygen agent is SODIUM PERCARBONATE, and SODIUM PERCARBONATE stability is poor, is easily decomposed during transport and use so that active o content drops
Low, SODIUM PERCARBONATE discharges the excessive velocities of oxygen in addition, causes to waste.
The content of the invention
The present invention is that there is provided one kind behaviour in order to solve the above mentioned problem present in the extra large crabbing keepalive method of prior art
Facilitate, it is with low cost, extra large crabbing survival rate and time-to-live can be effectively improved, with anhydrous packaged form realize extra large crabbing length away from
From the extra large crabbing waterless keep-alive method of, long-time On Transportaion of Live.
To achieve these goals, the present invention uses following technical scheme:
A kind of extra large crabbing waterless keep-alive method, comprises the following steps:
(1)Store up:After big chela plastic ties or the rubber band binding of the work sea crab of capture, it is placed in added with live keeping agent
Temporarily supported 1 ~ 2 day in seawater.The big chela of sea crab living is tied up to avoid mutually crushing between crab body, caused death, while
It is easy to the artificial operation in rear stage;The purpose stored up is to make the excreta in crab body emptying body, it is to avoid crab body discharge row during preservation
Thing is let out, microorganism amount reproduction is caused, the survival rate of crab body is reduced.
(2)It is classified anesthesia by freezing:Work sea crab after first storing up is placed in 1 ~ 2min of immersion in 7 ~ 8 DEG C of seawater, places into 1
1 ~ 2min is soaked in ~ 2 DEG C of seawater.Using the method for classification anesthesia by freezing, the temperature difference is small, can be prevented effectively from when anaesthetizing crab body because of temperature
Difference is excessive and produces disconnected foot or even dead phenomenon.
(3)Packaging:First in packing case bottom layer overlay oxygenation agent, a waterishlogged sea is then put on oxygenation agent
Silk floss, arranges last layer work sea crab on sponge, then puts a waterishlogged sponge, arranges one layer of sea crab living, puts a waterishlogged sea
Two layers of work sea crab is filled in silk floss, packing box altogether, packing case is simultaneously placed in preservation under 2 ~ 5 DEG C of environment by last joint sealing.Oxygenation agent is put
In the bottom, oxygen just can be slowly released when the water in sponge is contacted with oxygenation agent, it is ensured that sufficient oxygen supply, extend crab
The keep-alive time of body.
Preferably, step(1)In, the addition of live keeping agent is the 3 ~ 4% of seawer quality.
Preferably, the live keeping agent is made up of the component of following mass percent:5 ~ 10% magnesium sulfate, 1.5 ~ 3.5% chlorine
Change potassium, 2.5 ~ 6.5% gypsum, 7.5 ~ 15% Rosmarinic acids, 0.0025 ~ 0.0065% aldosterone, surplus is water.Guarantor in the present invention
Agent living is the key point for extending the crab body time-to-live, and wherein magnesium sulfate is used to promote crab body to discharge excreta;Potassium chloride is used to tie up
Crab body intracellular osmotic pressure and acid-base balance are held, to extend the time-to-live;Gypsum can suppress nerve to a certain extent stress be anti-
Should, vasopermeability is reduced, so as to suppress the activity of crab;Rosmarinic acid can adjust the activity of various metabolic enzymes in crab body, drop
Low consumption oxygen amount, increases the activity of antioxidase, so that improve crab picks up the adaptability stimulated during packaging anoxic from water, carries
High-survival rate;Aldosterone can suppress the metabolism of crab body, improve the adaptability of crab environment to external world, contribute to extension to survive
Time.Live keeping agent scientific formulation in the present invention is reasonable, by the coordinated between each component, when can extend the survival of crab
Between.
Preferably, step(3)In, the laying depth of the oxygenation agent is 1 ~ 2cm.
Preferably, the oxygenation agent is made by the following method:On the basis of oxygenation agent gross mass, by 5 ~ 10% vermiculites
Powder, 10 ~ 15% microcrystalline celluloses, 2 ~ 5% low-substituted hydroxypropyl celluloses, 2 ~ 5% lactose, surplus is the quality percentage of calper calcium peroxide
Content proportioning weighs each component, and each component is well mixed rear tabletted.Oxygenation agent is another pass of the present invention
Reaction generates oxygen and calcium hydroxide after key point, wherein calper calcium peroxide are contacted with the water in sponge, and the calcium hydroxide generated can
Absorb the carbon dioxide that crab body breathing is produced, it is to avoid space oxygen concentration is too low in packing box causes anoxic;Have in vermiculite power
Thousands of outer pores, with good absorption, sustained release and humidification, can slowly be gradually introduced oxygenation agent by outside moisture
Inside so as to ensure oxygenation agent can slow and thoroughly disintegration, and vermiculite power can adsorb the oxygen of generation so as to which effectively control increases
The oxygen rate of release of oxygen agent is to improve the utilization rate of oxygen;Microcrystalline cellulose plays a part of binding agent;Low substituted hydroxy-propyl
Cellulose plays a part of disintegrant;It with the addition of vermiculite power in the present invention, but vermiculite power poor compressibility, it is impossible to direct tablet compressing,
Lactose compressibility is good, therefore lactose is added in the present invention not only efficiently solves vermiculite power and can not directly carry out asking for compressing tablet
Topic, moreover it is possible to improve the compacted density of oxygenation agent, and make it that the oxygenation agent surface finishing being pressed into is attractive in appearance.
Therefore, the present invention has the advantages that:
(1)To crab body using classification anesthesia by freezing, the temperature difference is small, is produced when can be prevented effectively from anesthesia crab body because of excessive temperature differentials
Disconnected foot or even dead phenomenon;
(2)Live keeping agent is with the addition of when storing up in the seawater, and discloses the formula of live keeping agent, passes through each component in live keeping agent
Between coordinated, the time-to-live of crab can be extended;
(3)Optimize the formula of oxygenation agent and disclose the preparation method of oxygenation agent, can be disintegrated completely in guarantee oxygenation agent
On the premise of, the rate of release of oxygen is effectively controlled, so as to improve the utilization rate and using effect of oxygenation agent;
(4)It is easy to operate, it is with low cost, extra large crabbing survival rate and time-to-live can be greatly improved, with anhydrous packaged form
Realize extra large crabbing long range, long-time On Transportaion of Live, remarkable in economical benefits.
Embodiment
Below by embodiment, the present invention will be further described.
In the present invention, if not refering in particular to, all percentages are unit of weight, and all devices and raw material can be purchased from market
Or the industry it is conventional, the method in following embodiments unless otherwise instructed, is this area conventional method.
Embodiment 1
(1)Store up:By the work sea crab of capture(Portunus trituberculatus Miers)Big chela bundled with plastic ties after, be placed in the promising sea of addition
Temporarily supported 1 day in the seawater of the live keeping agent of water quality 4%, live keeping agent is made up of the component of following mass percent:10% magnesium sulfate,
3.5% potassium chloride, 6.5% gypsum, 15% Rosmarinic acid, 0.0065% aldosterone, surplus is water.
(2)It is classified anesthesia by freezing:Work sea crab after first storing up, which is placed in 8 DEG C of seawater, soaks 2min, places into 2 DEG C
2min is soaked in seawater.
(3)Packaging:First in the oxygenation agent that packing case bottom layer overlay thickness is 2cm, one is then put on oxygenation agent
Waterishlogged sponge is opened, last layer work sea crab is arranged on sponge, then puts a waterishlogged sponge, one layer of sea crab living is arranged, puts one
Open and fill two layers of Portunus trituberculatus Miers in waterishlogged sponge, packing box altogether, packing case is simultaneously placed under 5 DEG C of environment and preserved by last joint sealing
, wherein oxygenation agent is made by the following method:On the basis of oxygenation agent gross mass, by 10% vermiculite power, 15% microcrystalline cellulose
Element, 5% low-substituted hydroxypropyl cellulose, 5% lactose, surplus weighs each component for the weight/mass percentage composition proportioning of calper calcium peroxide, will
It is tabletted after each component is well mixed.
Embodiment 2
(1)Store up:By the work sea crab of capture(Sea crab)Big chela bundled with rubber band after, be placed in the promising seawer quality 3% of addition
Live keeping agent seawater in temporarily support 2 days, live keeping agent is made up of the component of following mass percent:5% magnesium sulfate, 1.5% potassium chloride,
2.5% gypsum, 7.5% Rosmarinic acid, 0.0025% aldosterone, surplus is water.
(2)It is classified anesthesia by freezing:Work sea crab after first storing up, which is placed in 7 DEG C of seawater, soaks 1min, places into 1 DEG C
1min is soaked in seawater.
(3)Packaging:First in the oxygenation agent that packing case bottom layer overlay thickness is 1cm, one is then put on oxygenation agent
Waterishlogged sponge is opened, last layer work sea crab is arranged on sponge, then puts a waterishlogged sponge, one layer of sea crab living is arranged, puts one
Open and fill two layers of Sea crab in waterishlogged sponge, packing box altogether, packing case is simultaneously placed under 2 DEG C of environment and preserved by last joint sealing,
Wherein oxygenation agent is made by the following method:On the basis of oxygenation agent gross mass, by 5% vermiculite power, 15% microcrystalline cellulose, 5% is low
Replace hydroxypropyl cellulose, 5% lactose, surplus weighs each component for the weight/mass percentage composition proportioning of calper calcium peroxide, each component is mixed
Close uniform rear tabletted.
Embodiment 3
(1)Store up:By the work sea crab of capture(Mud crab)Big chela bundled with plastic ties after, be placed in the promising seawater of addition
Temporarily supported 1.5 days in the seawater of the live keeping agent of quality 3.5%, live keeping agent is made up of the component of following mass percent:6% magnesium sulfate,
3% potassium chloride, 4% gypsum, 10% Rosmarinic acid, 0.005% aldosterone, surplus is water.
(2)It is classified anesthesia by freezing:Work sea crab after first storing up, which is placed in 7.5 DEG C of seawater, soaks 1.5min, places into
1.5min is soaked in 1.5 DEG C of seawater.
(3)Packaging:First in the oxygenation agent that packing case bottom layer overlay thickness is 1.2cm, then put on oxygenation agent
One waterishlogged sponge, arranges last layer work sea crab on sponge, then puts a waterishlogged sponge, arranges one layer of sea crab living, puts
Two layers of mud crab is filled in one waterishlogged sponge, packing box altogether, packing case is simultaneously placed in preservation under 3 DEG C of environment by last joint sealing
, wherein oxygenation agent is made by the following method:On the basis of oxygenation agent gross mass, by 6% vermiculite power, 12% microcrystalline cellulose
Element, 3% low-substituted hydroxypropyl cellulose, 3% lactose, surplus weighs each component for the weight/mass percentage composition proportioning of calper calcium peroxide, will
It is tabletted after each component is well mixed.
The present invention is easy to operate, with low cost, keep-alive is carried out to extra large crabbing using the method for the present invention, during extra large crabbing survival
Between up to more than 72 hours(2 ~ 5 DEG C of temperature), survival rate is more than 95% after keep-alive 72 hours, and the keep-alive time greatly prolongs, with anhydrous
Packaged form realizes sea crab long range living, long-time On Transportaion of Live, and remarkable in economical benefits is adapted to popularization and application.
Embodiment described above is a kind of preferably scheme of the present invention, not makees any formal to the present invention
Limitation, also has other variants and remodeling on the premise of without departing from the technical scheme described in claim.
Claims (2)
1. a kind of extra large crabbing waterless keep-alive method, it is characterised in that comprise the following steps:
(1)Store up:After big chela plastic ties or the rubber band binding of the work sea crab of capture, the seawater added with live keeping agent is placed in
In temporarily support 1 ~ 2 day, the addition of live keeping agent is the 3 ~ 4% of seawer quality, and live keeping agent is made up of the component of following mass percent:5
~ 10% magnesium sulfate, 1.5 ~ 3.5% potassium chloride, 2.5 ~ 6.5% gypsum, 7.5 ~ 15% Rosmarinic acids, 0.0025 ~ 0.0065% aldosterone,
Surplus is water;
(2)It is classified anesthesia by freezing:Work sea crab after first storing up is placed in 1 ~ 2min of immersion in 7 ~ 8 DEG C of seawater, places into 1 ~ 2 DEG C
Seawater in soak 1 ~ 2min;
(3)Packaging:First in packing case bottom layer overlay oxygenation agent, a waterishlogged sponge is then put on oxygenation agent,
Last layer work sea crab is arranged on sponge, then puts a waterishlogged sponge, one layer of sea crab living is arranged, puts a waterishlogged sponge,
Fill two layers of work sea crab in packing box altogether, last joint sealing and packing case is placed under 2 ~ 5 DEG C of environment is preserved, oxygenation agent by with
Lower section method is made:On the basis of oxygenation agent gross mass, by 5 ~ 10% vermiculite powers, 10 ~ 15% microcrystalline celluloses, 2 ~ 5% low-substituted hydroxypropyls
Base cellulose, 2 ~ 5% lactose, surplus weighs each component for the weight/mass percentage composition proportioning of calper calcium peroxide, and each component is well mixed
It is tabletted afterwards.
2. a kind of extra large crabbing waterless keep-alive method according to claim 1, it is characterised in that step(3)In, the oxygenation
The laying depth of agent is 1 ~ 2cm.
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Families Citing this family (6)
Publication number | Priority date | Publication date | Assignee | Title |
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CN105706983A (en) * | 2016-03-16 | 2016-06-29 | 上海海洋大学 | Waterless keep-alive transportation method of live shrimps |
CN107223616A (en) * | 2016-03-23 | 2017-10-03 | 射阳县泰和水产品有限公司 | A kind of waterless keep-alive method of Portunus trituberculatus Miers |
CN105941596A (en) * | 2016-05-18 | 2016-09-21 | 青岛博智汇力生物科技有限公司 | Aquatic product temporary rearing and living keeping agent capable of removing heavy metal, use method and application |
CN106106320B (en) * | 2016-08-01 | 2019-03-05 | 江苏大学 | A kind of oxygen slow release device for sturgeon low temperature On Transportaion of Live device |
CN106804516A (en) * | 2016-12-21 | 2017-06-09 | 浙江海洋大学 | A kind of method for improving the black crab long-distance transport survival rate of Burma |
CN107996479A (en) * | 2017-12-04 | 2018-05-08 | 浙江海洋大学 | A kind of fresh-keeping and alive-keeping method of swimming crab |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1261514A (en) * | 1998-12-25 | 2000-08-02 | 三共株式会社 | Agricultural chemical composition for water surface |
CN1596631A (en) * | 2004-07-21 | 2005-03-23 | 张南伟 | Granular oxygenation agent for fish and shrimp and its preparation method |
CN201557462U (en) * | 2009-09-16 | 2010-08-25 | 许庆华 | Oxygenated live lobster transport case |
CN102084836A (en) * | 2010-11-12 | 2011-06-08 | 伊焱垚 | Method for refreshing water-detacted live portunus tritubercularus |
CN102531072A (en) * | 2010-12-09 | 2012-07-04 | 王海炜 | Oxygen increasing agent |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR200444126Y1 (en) * | 2007-06-20 | 2009-04-17 | 이재우 | Raising Safeguard Equipment A Crab Safeguard |
-
2014
- 2014-12-26 CN CN201410824329.XA patent/CN104885974B/en active Active
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1261514A (en) * | 1998-12-25 | 2000-08-02 | 三共株式会社 | Agricultural chemical composition for water surface |
CN1596631A (en) * | 2004-07-21 | 2005-03-23 | 张南伟 | Granular oxygenation agent for fish and shrimp and its preparation method |
CN201557462U (en) * | 2009-09-16 | 2010-08-25 | 许庆华 | Oxygenated live lobster transport case |
CN102084836A (en) * | 2010-11-12 | 2011-06-08 | 伊焱垚 | Method for refreshing water-detacted live portunus tritubercularus |
CN102531072A (en) * | 2010-12-09 | 2012-07-04 | 王海炜 | Oxygen increasing agent |
Non-Patent Citations (2)
Title |
---|
"三疣梭子蟹低温保活";林国雄等;《海洋渔业》;19840430(第2期);第30页 * |
"蟹虾低温存活保活剂的制作与使用";傅生;《吉林农业》;20060430(第8期);第61-62页 * |
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