CN104830852B - 一种检测hla‑b*15:02等位基因的多重实时荧光pcr方法 - Google Patents
一种检测hla‑b*15:02等位基因的多重实时荧光pcr方法 Download PDFInfo
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CN106520971A (zh) * | 2016-11-25 | 2017-03-22 | 武汉海吉力生物科技有限公司 | 用于快速检测hla‑b*1502等位基因的核酸、试剂盒及方法 |
CN106755530B (zh) * | 2017-02-24 | 2021-01-01 | 陕西佰美基因股份有限公司 | 一种检测hla-a*31:01等位基因的mgb探针实时荧光pcr方法及其引物探针组合 |
CN109055366A (zh) * | 2018-09-05 | 2018-12-21 | 武汉康录生物技术股份有限公司 | 一种人类哮喘风险基因多态性检测试剂盒及其制备方法和应用 |
CN112458156A (zh) * | 2020-12-07 | 2021-03-09 | 西北大学 | 一种检测hla-b*15:02等位基因的荧光pcr方法及特异性引物探针组合 |
CN113151435A (zh) * | 2020-12-16 | 2021-07-23 | 杭州百迈生物股份有限公司 | 一种定性检测hla-b*1502等位基因的试剂盒及方法 |
CN112538527B (zh) * | 2020-12-25 | 2023-11-03 | 郑州安图生物工程股份有限公司 | 用于检测人类白细胞抗原b位点1502基因的引物和探针组合及试剂盒 |
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Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101353698A (zh) * | 2008-03-21 | 2009-01-28 | 广州医学院第二附属医院 | 检测抗癫痫药物过敏反应相关抗原基因型的试剂及临床应用方法 |
CN102296109A (zh) * | 2011-06-30 | 2011-12-28 | 北京思尔成生物技术有限公司 | HLA-B*1502基因的SYBR Green I检测方法及其专用引物与试剂盒 |
CN103820543A (zh) * | 2014-01-27 | 2014-05-28 | 希斯奇生物医药(上海)有限公司 | Hla-b*15:02等位基因检测方法及其试剂盒 |
CN103114130B (zh) * | 2012-11-29 | 2014-07-09 | 瀚吉康生物科技(北京)有限公司 | 检测人白细胞抗原hla-b*1502 基因型的试剂盒及方法 |
CN104450914A (zh) * | 2014-12-10 | 2015-03-25 | 苏州旷远生物分子技术有限公司 | 用于检测人hla-b*1502基因的引物、探针、荧光pcr试剂盒和方法 |
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CN101353698A (zh) * | 2008-03-21 | 2009-01-28 | 广州医学院第二附属医院 | 检测抗癫痫药物过敏反应相关抗原基因型的试剂及临床应用方法 |
CN102296109A (zh) * | 2011-06-30 | 2011-12-28 | 北京思尔成生物技术有限公司 | HLA-B*1502基因的SYBR Green I检测方法及其专用引物与试剂盒 |
CN103114130B (zh) * | 2012-11-29 | 2014-07-09 | 瀚吉康生物科技(北京)有限公司 | 检测人白细胞抗原hla-b*1502 基因型的试剂盒及方法 |
CN103820543A (zh) * | 2014-01-27 | 2014-05-28 | 希斯奇生物医药(上海)有限公司 | Hla-b*15:02等位基因检测方法及其试剂盒 |
CN104450914A (zh) * | 2014-12-10 | 2015-03-25 | 苏州旷远生物分子技术有限公司 | 用于检测人hla-b*1502基因的引物、探针、荧光pcr试剂盒和方法 |
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Denomination of invention: A multiplex real-time fluorescent PCR method for detecting HLA-B * 15:02 alleles Effective date of registration: 20200609 Granted publication date: 20180406 Pledgee: Xi'an Science and Technology Financial Service Center Co.,Ltd. Pledgor: SHAANXI LIFEGEN Co.,Ltd. Registration number: Y2020610000063 |
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Denomination of invention: A multiplex real-time fluorescent PCR method for detecting HLA-B * 15:02 alleles Effective date of registration: 20210819 Granted publication date: 20180406 Pledgee: Bank of Chongqing Co.,Ltd. Xi'an Qujiang New Area sub branch Pledgor: SHAANXI LIFEGEN Co.,Ltd. Registration number: Y2021980007995 |
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Date of cancellation: 20221122 Granted publication date: 20180406 Pledgee: Bank of Chongqing Co.,Ltd. Xi'an Qujiang New Area sub branch Pledgor: SHAANXI LIFEGEN Co.,Ltd. Registration number: Y2021980007995 |