CN104826673A - 写入式二维微流控液滴阵列化装置、用途及其使用方法 - Google Patents
写入式二维微流控液滴阵列化装置、用途及其使用方法 Download PDFInfo
- Publication number
- CN104826673A CN104826673A CN201510113161.6A CN201510113161A CN104826673A CN 104826673 A CN104826673 A CN 104826673A CN 201510113161 A CN201510113161 A CN 201510113161A CN 104826673 A CN104826673 A CN 104826673A
- Authority
- CN
- China
- Prior art keywords
- drop
- write
- array
- droplet array
- attachment device
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 title claims abstract description 57
- 230000033001 locomotion Effects 0.000 claims abstract description 34
- 239000007788 liquid Substances 0.000 claims abstract description 13
- 238000003860 storage Methods 0.000 claims abstract description 11
- 238000004445 quantitative analysis Methods 0.000 claims abstract description 3
- 230000015572 biosynthetic process Effects 0.000 claims description 37
- 238000013519 translation Methods 0.000 claims description 18
- 239000002480 mineral oil Substances 0.000 claims description 14
- 235000010446 mineral oil Nutrition 0.000 claims description 14
- 238000002444 silanisation Methods 0.000 claims description 14
- 238000012216 screening Methods 0.000 claims description 11
- 230000008878 coupling Effects 0.000 claims description 9
- 238000010168 coupling process Methods 0.000 claims description 9
- 238000005859 coupling reaction Methods 0.000 claims description 9
- 238000002360 preparation method Methods 0.000 claims description 5
- 230000003287 optical effect Effects 0.000 claims description 2
- 241000894006 Bacteria Species 0.000 abstract description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 12
- 238000006243 chemical reaction Methods 0.000 abstract description 10
- 230000012010 growth Effects 0.000 abstract description 10
- 238000012544 monitoring process Methods 0.000 abstract description 6
- 239000013078 crystal Substances 0.000 abstract description 3
- 238000004113 cell culture Methods 0.000 abstract description 2
- 238000007847 digital PCR Methods 0.000 abstract description 2
- 238000002955 isolation Methods 0.000 abstract 1
- 239000008346 aqueous phase Substances 0.000 description 40
- 239000012071 phase Substances 0.000 description 35
- 239000003921 oil Substances 0.000 description 23
- 238000004458 analytical method Methods 0.000 description 19
- 238000012360 testing method Methods 0.000 description 14
- 230000008569 process Effects 0.000 description 13
- 239000004809 Teflon Substances 0.000 description 12
- 229920006362 Teflon® Polymers 0.000 description 12
- 238000001514 detection method Methods 0.000 description 12
- 230000008859 change Effects 0.000 description 11
- 238000013461 design Methods 0.000 description 11
- 239000000203 mixture Substances 0.000 description 11
- 241000588724 Escherichia coli Species 0.000 description 10
- 238000005520 cutting process Methods 0.000 description 10
- 238000000605 extraction Methods 0.000 description 10
- 238000002347 injection Methods 0.000 description 10
- 239000007924 injection Substances 0.000 description 10
- 230000003115 biocidal effect Effects 0.000 description 9
- 238000005516 engineering process Methods 0.000 description 8
- 239000001963 growth medium Substances 0.000 description 8
- 239000000243 solution Substances 0.000 description 8
- 238000010586 diagram Methods 0.000 description 7
- 239000000284 extract Substances 0.000 description 7
- 238000000926 separation method Methods 0.000 description 7
- 230000003321 amplification Effects 0.000 description 6
- 230000008901 benefit Effects 0.000 description 6
- 210000004027 cell Anatomy 0.000 description 6
- 239000003153 chemical reaction reagent Substances 0.000 description 6
- 238000000520 microinjection Methods 0.000 description 6
- 238000012986 modification Methods 0.000 description 6
- 230000004048 modification Effects 0.000 description 6
- 238000003199 nucleic acid amplification method Methods 0.000 description 6
- 230000001105 regulatory effect Effects 0.000 description 6
- 108010040201 Polymyxins Proteins 0.000 description 5
- 230000001580 bacterial effect Effects 0.000 description 5
- 230000004907 flux Effects 0.000 description 5
- 238000012545 processing Methods 0.000 description 5
- JQXXHWHPUNPDRT-WLSIYKJHSA-N rifampicin Chemical compound O([C@](C1=O)(C)O/C=C/[C@@H]([C@H]([C@@H](OC(C)=O)[C@H](C)[C@H](O)[C@H](C)[C@@H](O)[C@@H](C)\C=C\C=C(C)/C(=O)NC=2C(O)=C3C([O-])=C4C)C)OC)C4=C1C3=C(O)C=2\C=N\N1CC[NH+](C)CC1 JQXXHWHPUNPDRT-WLSIYKJHSA-N 0.000 description 5
- 229960001225 rifampicin Drugs 0.000 description 5
- 239000004793 Polystyrene Substances 0.000 description 4
- 238000003491 array Methods 0.000 description 4
- 230000001276 controlling effect Effects 0.000 description 4
- 238000009826 distribution Methods 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 239000012530 fluid Substances 0.000 description 4
- 238000007689 inspection Methods 0.000 description 4
- 239000011159 matrix material Substances 0.000 description 4
- 238000002156 mixing Methods 0.000 description 4
- 230000036961 partial effect Effects 0.000 description 4
- 229920002223 polystyrene Polymers 0.000 description 4
- TUGGFUSCPLPUFY-UHFFFAOYSA-N 3-triethoxysilylpropan-1-amine Chemical group CCO[Si](OCC)(OCC)CCCN.CCO[Si](OCC)(OCC)CCCN TUGGFUSCPLPUFY-UHFFFAOYSA-N 0.000 description 3
- 108010043121 Green Fluorescent Proteins Proteins 0.000 description 3
- XIURVHNZVLADCM-IUODEOHRSA-N cefalotin Chemical compound N([C@H]1[C@@H]2N(C1=O)C(=C(CS2)COC(=O)C)C(O)=O)C(=O)CC1=CC=CS1 XIURVHNZVLADCM-IUODEOHRSA-N 0.000 description 3
- 229960000603 cefalotin Drugs 0.000 description 3
- 238000011161 development Methods 0.000 description 3
- 230000018109 developmental process Effects 0.000 description 3
- 238000006073 displacement reaction Methods 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 239000005090 green fluorescent protein Substances 0.000 description 3
- 238000003384 imaging method Methods 0.000 description 3
- 102000039446 nucleic acids Human genes 0.000 description 3
- 108020004707 nucleic acids Proteins 0.000 description 3
- 150000007523 nucleic acids Chemical class 0.000 description 3
- 238000004321 preservation Methods 0.000 description 3
- 108010054624 red fluorescent protein Proteins 0.000 description 3
- 239000004094 surface-active agent Substances 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 102000004144 Green Fluorescent Proteins Human genes 0.000 description 2
- 238000012408 PCR amplification Methods 0.000 description 2
- BLRPTPMANUNPDV-UHFFFAOYSA-N Silane Chemical compound [SiH4] BLRPTPMANUNPDV-UHFFFAOYSA-N 0.000 description 2
- 238000002679 ablation Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 238000012742 biochemical analysis Methods 0.000 description 2
- 238000004590 computer program Methods 0.000 description 2
- 239000000470 constituent Substances 0.000 description 2
- 238000013499 data model Methods 0.000 description 2
- 239000004205 dimethyl polysiloxane Substances 0.000 description 2
- 235000013870 dimethyl polysiloxane Nutrition 0.000 description 2
- 230000002401 inhibitory effect Effects 0.000 description 2
- 239000010410 layer Substances 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- CXQXSVUQTKDNFP-UHFFFAOYSA-N octamethyltrisiloxane Chemical compound C[Si](C)(C)O[Si](C)(C)O[Si](C)(C)C CXQXSVUQTKDNFP-UHFFFAOYSA-N 0.000 description 2
- 230000002093 peripheral effect Effects 0.000 description 2
- 238000004987 plasma desorption mass spectroscopy Methods 0.000 description 2
- 239000004033 plastic Substances 0.000 description 2
- 229920000435 poly(dimethylsiloxane) Polymers 0.000 description 2
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 238000007790 scraping Methods 0.000 description 2
- 229910000077 silane Inorganic materials 0.000 description 2
- 239000002356 single layer Substances 0.000 description 2
- 238000004381 surface treatment Methods 0.000 description 2
- 230000005514 two-phase flow Effects 0.000 description 2
- 240000001439 Opuntia Species 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 150000001335 aliphatic alkanes Chemical class 0.000 description 1
- 238000000137 annealing Methods 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 230000004087 circulation Effects 0.000 description 1
- 238000005253 cladding Methods 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000003759 clinical diagnosis Methods 0.000 description 1
- 238000010367 cloning Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000004132 cross linking Methods 0.000 description 1
- 210000004748 cultured cell Anatomy 0.000 description 1
- 230000001186 cumulative effect Effects 0.000 description 1
- 125000004122 cyclic group Chemical group 0.000 description 1
- 150000001924 cycloalkanes Chemical class 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000005137 deposition process Methods 0.000 description 1
- 238000003113 dilution method Methods 0.000 description 1
- YQGOWXYZDLJBFL-UHFFFAOYSA-N dimethoxysilane Chemical compound CO[SiH2]OC YQGOWXYZDLJBFL-UHFFFAOYSA-N 0.000 description 1
- 231100000673 dose–response relationship Toxicity 0.000 description 1
- 238000011304 droplet digital PCR Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000007877 drug screening Methods 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 239000000839 emulsion Substances 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 238000000105 evaporative light scattering detection Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 238000000799 fluorescence microscopy Methods 0.000 description 1
- 239000007850 fluorescent dye Substances 0.000 description 1
- 230000009036 growth inhibition Effects 0.000 description 1
- 230000002209 hydrophobic effect Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 230000004807 localization Effects 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000001819 mass spectrum Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000001869 matrix assisted laser desorption--ionisation mass spectrum Methods 0.000 description 1
- 238000001840 matrix-assisted laser desorption--ionisation time-of-flight mass spectrometry Methods 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- 238000001259 photo etching Methods 0.000 description 1
- 238000003752 polymerase chain reaction Methods 0.000 description 1
- 238000003825 pressing Methods 0.000 description 1
- 238000003672 processing method Methods 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 238000010926 purge Methods 0.000 description 1
- 238000011002 quantification Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 229920006395 saturated elastomer Polymers 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 238000002174 soft lithography Methods 0.000 description 1
- 238000007711 solidification Methods 0.000 description 1
- 230000008023 solidification Effects 0.000 description 1
- 238000003892 spreading Methods 0.000 description 1
- 230000007480 spreading Effects 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
- 239000012089 stop solution Substances 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- 230000036962 time dependent Effects 0.000 description 1
- 230000025366 tissue development Effects 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
Abstract
Description
Claims (14)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201510113161.6A CN104826673B (zh) | 2015-03-16 | 2015-03-16 | 写入式二维微流控液滴阵列化装置、用途及其使用方法 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201510113161.6A CN104826673B (zh) | 2015-03-16 | 2015-03-16 | 写入式二维微流控液滴阵列化装置、用途及其使用方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN104826673A true CN104826673A (zh) | 2015-08-12 |
CN104826673B CN104826673B (zh) | 2017-06-23 |
Family
ID=53805081
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201510113161.6A Active CN104826673B (zh) | 2015-03-16 | 2015-03-16 | 写入式二维微流控液滴阵列化装置、用途及其使用方法 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN104826673B (zh) |
Cited By (14)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106635796A (zh) * | 2015-10-30 | 2017-05-10 | 中国科学院微生物研究所 | 用于细胞固着培养的装置及固着培养方法 |
CN106841151A (zh) * | 2017-03-20 | 2017-06-13 | 广东顺德工业设计研究院(广东顺德创新设计研究院) | 微流控芯片及其微流道结构和液态微滴的检测方法 |
CN107475074A (zh) * | 2017-09-12 | 2017-12-15 | 深圳市尚维高科有限公司 | 微流控pcr芯片 |
CN108680970A (zh) * | 2018-03-26 | 2018-10-19 | 上海理工大学 | 一种微透镜阵列及其微流控芯片的制作方法及系统 |
CN108949496A (zh) * | 2017-05-18 | 2018-12-07 | 中国科学院大连化学物理研究所 | 一种基于液滴微流控芯片的单细胞分离方法 |
CN109308712A (zh) * | 2017-07-26 | 2019-02-05 | 清华大学 | 一种从液滴流视频中计算液滴运动频率的方法 |
EP3482828A1 (en) * | 2017-11-08 | 2019-05-15 | Oxford University Innovation Ltd. | Methods and apparatus for adding or removing material from a microfluidic arrangement |
CN110292962A (zh) * | 2019-07-12 | 2019-10-01 | 湖南圣湘生物科技有限公司 | 液体吸取控制方法、装置、设备、系统及存储介质 |
CN110295109A (zh) * | 2019-07-08 | 2019-10-01 | 中国科学院深圳先进技术研究院 | 基于微流控液滴打印系统的数字pcr检测方法及应用 |
CN111135883A (zh) * | 2019-12-31 | 2020-05-12 | 中山大学 | 一种用于筛选晶体生成条件的超高通量平台及筛选方法 |
CN112138734A (zh) * | 2020-09-26 | 2020-12-29 | 宁波华仪宁创智能科技有限公司 | 液滴的产生方法及装置 |
CN112909215A (zh) * | 2021-01-29 | 2021-06-04 | 中国科学院长春光学精密机械与物理研究所 | 量子点色转换阵列的制备方法 |
CN112999997A (zh) * | 2021-02-20 | 2021-06-22 | 嘉兴学院 | 一种实现液滴运动路径可控的装置及方法 |
WO2021120451A1 (zh) * | 2019-12-17 | 2021-06-24 | 北京大学 | 一种有机质谱流式细胞分析技术 |
Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1997044134A1 (en) * | 1996-05-17 | 1997-11-27 | Incyte Pharmaceuticals, Inc. | Jet droplet device and method |
US6110426A (en) * | 1994-06-17 | 2000-08-29 | The Board Of Trustees Of The Leland Stanford Junior University | Methods for fabricating microarrays of biological samples |
US20050156056A1 (en) * | 2002-10-15 | 2005-07-21 | Larson Bradley J. | Methods and apparata for precisely dispensing microvolumes of fluids |
CN1986229A (zh) * | 2005-12-23 | 2007-06-27 | 章维一 | 基因芯片点样喷头 |
CN103217324A (zh) * | 2013-04-10 | 2013-07-24 | 上海裕隆生物科技有限公司 | 一种生物芯片点样仪 |
CN103954786A (zh) * | 2014-04-21 | 2014-07-30 | 浙江大学 | 一种半接触式的油下液滴连续点样和加液方法 |
-
2015
- 2015-03-16 CN CN201510113161.6A patent/CN104826673B/zh active Active
Patent Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6110426A (en) * | 1994-06-17 | 2000-08-29 | The Board Of Trustees Of The Leland Stanford Junior University | Methods for fabricating microarrays of biological samples |
WO1997044134A1 (en) * | 1996-05-17 | 1997-11-27 | Incyte Pharmaceuticals, Inc. | Jet droplet device and method |
US20050156056A1 (en) * | 2002-10-15 | 2005-07-21 | Larson Bradley J. | Methods and apparata for precisely dispensing microvolumes of fluids |
CN1986229A (zh) * | 2005-12-23 | 2007-06-27 | 章维一 | 基因芯片点样喷头 |
CN103217324A (zh) * | 2013-04-10 | 2013-07-24 | 上海裕隆生物科技有限公司 | 一种生物芯片点样仪 |
CN103954786A (zh) * | 2014-04-21 | 2014-07-30 | 浙江大学 | 一种半接触式的油下液滴连续点样和加液方法 |
Cited By (23)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106635796B (zh) * | 2015-10-30 | 2019-08-30 | 中国科学院微生物研究所 | 用于细胞固着培养的装置及固着培养方法 |
CN106635796A (zh) * | 2015-10-30 | 2017-05-10 | 中国科学院微生物研究所 | 用于细胞固着培养的装置及固着培养方法 |
CN106841151A (zh) * | 2017-03-20 | 2017-06-13 | 广东顺德工业设计研究院(广东顺德创新设计研究院) | 微流控芯片及其微流道结构和液态微滴的检测方法 |
CN108949496A (zh) * | 2017-05-18 | 2018-12-07 | 中国科学院大连化学物理研究所 | 一种基于液滴微流控芯片的单细胞分离方法 |
CN109308712B (zh) * | 2017-07-26 | 2021-10-26 | 清华大学 | 一种从液滴流视频中计算液滴运动频率的方法 |
CN109308712A (zh) * | 2017-07-26 | 2019-02-05 | 清华大学 | 一种从液滴流视频中计算液滴运动频率的方法 |
CN107475074A (zh) * | 2017-09-12 | 2017-12-15 | 深圳市尚维高科有限公司 | 微流控pcr芯片 |
CN107475074B (zh) * | 2017-09-12 | 2024-04-05 | 深圳市尚维高科有限公司 | 微流控pcr芯片 |
EP3482828A1 (en) * | 2017-11-08 | 2019-05-15 | Oxford University Innovation Ltd. | Methods and apparatus for adding or removing material from a microfluidic arrangement |
WO2019091753A1 (en) * | 2017-11-08 | 2019-05-16 | Oxford University Innovation Limited | Methods and apparatus for adding or removing material from a microfluidic arrangement |
CN108680970A (zh) * | 2018-03-26 | 2018-10-19 | 上海理工大学 | 一种微透镜阵列及其微流控芯片的制作方法及系统 |
CN108680970B (zh) * | 2018-03-26 | 2019-12-06 | 上海理工大学 | 一种微透镜阵列及其微流控芯片的制作方法及系统 |
CN110295109A (zh) * | 2019-07-08 | 2019-10-01 | 中国科学院深圳先进技术研究院 | 基于微流控液滴打印系统的数字pcr检测方法及应用 |
CN110292962B (zh) * | 2019-07-12 | 2023-04-07 | 圣湘生物科技股份有限公司 | 液体吸取控制方法、装置、设备、系统及存储介质 |
CN110292962A (zh) * | 2019-07-12 | 2019-10-01 | 湖南圣湘生物科技有限公司 | 液体吸取控制方法、装置、设备、系统及存储介质 |
WO2021120451A1 (zh) * | 2019-12-17 | 2021-06-24 | 北京大学 | 一种有机质谱流式细胞分析技术 |
WO2021135090A1 (zh) * | 2019-12-31 | 2021-07-08 | 中山大学 | 一种用于筛选晶体生成条件的超高通量平台及筛选方法 |
CN111135883A (zh) * | 2019-12-31 | 2020-05-12 | 中山大学 | 一种用于筛选晶体生成条件的超高通量平台及筛选方法 |
CN111135883B (zh) * | 2019-12-31 | 2024-01-02 | 中山大学 | 一种用于筛选晶体生成条件的超高通量平台及筛选方法 |
CN112138734A (zh) * | 2020-09-26 | 2020-12-29 | 宁波华仪宁创智能科技有限公司 | 液滴的产生方法及装置 |
CN112909215A (zh) * | 2021-01-29 | 2021-06-04 | 中国科学院长春光学精密机械与物理研究所 | 量子点色转换阵列的制备方法 |
CN112999997A (zh) * | 2021-02-20 | 2021-06-22 | 嘉兴学院 | 一种实现液滴运动路径可控的装置及方法 |
CN112999997B (zh) * | 2021-02-20 | 2022-04-15 | 嘉兴学院 | 一种实现液滴运动路径可控的装置及方法 |
Also Published As
Publication number | Publication date |
---|---|
CN104826673B (zh) | 2017-06-23 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN104826673B (zh) | 写入式二维微流控液滴阵列化装置、用途及其使用方法 | |
CN103954786B (zh) | 一种半接触式的油下液滴连续点样方法 | |
CN105164246B (zh) | 用于分析定义的多细胞组合的方法和设备 | |
Wang et al. | Digital microfluidics: A promising technique for biochemical applications | |
WO2016078339A1 (zh) | 微液滴生成装置、系统、方法及单细胞/单分子分析装置 | |
US9804185B2 (en) | Application method for automatic micro droplet array screening system with picoliter scale precision | |
EP2621631B1 (en) | Microfluidic device for production and collection of droplets of a fluid | |
Kaigala et al. | Microfluidics in the “open space” for performing localized chemistry on biological interfaces | |
JP2021176312A (ja) | 細胞を単離し分析するためのシステムおよび方法 | |
WO2021041709A1 (en) | Methods and systems for droplet manipulation | |
CA2808118C (en) | Composite liquid cells | |
CN110579616B (zh) | 一种微液滴处理装置及其使用方法 | |
US20070036690A1 (en) | Inlet channel volume in a reactor | |
CN107513495A (zh) | 用于核酸检测的多通道微滴检测芯片 | |
CN104492508A (zh) | 一种基于液体残留的超微量液滴操控装置及方法 | |
US11745183B2 (en) | Microtiter plate and uses thereof | |
CN1330154A (zh) | 细胞微阵列芯片及其制备方法 | |
JP2021513363A (ja) | 灌流可能なバイオリアクタ | |
CN104497099A (zh) | 一种气相扩散型结晶芯片及使用方法 | |
JP4423150B2 (ja) | 液滴サイズの制御装置ならびに制御方法 | |
JP4679847B2 (ja) | 細胞の解析方法 | |
Zhang et al. | Bent-capillary-centrifugal-driven monodisperse droplet generator with its application for digital LAMP assay | |
CN110577888B (zh) | 一种微流控芯片及包括其的系统 | |
CN207362225U (zh) | 用于核酸检测的多通道微滴检测芯片 | |
CN106256436A (zh) | 气体间隔式防液滴蒸发的微流控芯片装置及方法 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
EXSB | Decision made by sipo to initiate substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
GR01 | Patent grant | ||
GR01 | Patent grant | ||
TR01 | Transfer of patent right | ||
TR01 | Transfer of patent right |
Effective date of registration: 20200529 Address after: 100176 Daxing District Beijing economic and Technological Development Zone, Zhong Sheng Road, No. 2 hospital Patentee after: HOLLYSYS AUTOMATION TECHNOLOGIES Ltd. Address before: 100101 No. 1, West Beichen Road, Beijing, Chaoyang District, 3 Patentee before: INSTITUTE OF MICROBIOLOGY, CHINESE ACADEMY OF SCIENCES |
|
TR01 | Transfer of patent right |
Effective date of registration: 20200728 Address after: 100085 Zone B, Zhongguancun Biomedical Park, No. 5 Pioneer Road, Haidian District, Beijing Patentee after: Beijing Da Microbiology Technology Co.,Ltd. Address before: 100176 Daxing District Beijing economic and Technological Development Zone, Zhong Sheng Road, No. 2 hospital Patentee before: HOLLYSYS AUTOMATION TECHNOLOGIES Ltd. |
|
TR01 | Transfer of patent right | ||
TR01 | Transfer of patent right |
Effective date of registration: 20231007 Address after: 611731 building 4, No.8, Anhe 2nd Road, hi tech Zone, Chengdu, Sichuan Province Patentee after: MACCURA MEDICAL ELECTRONICS Co.,Ltd. Address before: 100085 106, area B, Zhongguancun biomedical park, No.5 Kaifa Road, Haidian District, Beijing Patentee before: Beijing Da Microbiology Technology Co.,Ltd. |
|
TR01 | Transfer of patent right | ||
TR01 | Transfer of patent right |
Effective date of registration: 20231219 Address after: 611731, 16 Chengdu, hi tech Zone, Sichuan, China Patentee after: MACCURA BIOTECHNOLOGY Co.,Ltd. Address before: 611731 building 4, No.8, Anhe 2nd Road, hi tech Zone, Chengdu, Sichuan Province Patentee before: MACCURA MEDICAL ELECTRONICS Co.,Ltd. |
|
TR01 | Transfer of patent right |