CN104825622A - Application of traditional Chinese medicine composition in preparation of drug used for resisting influenza A (H3N2) virus - Google Patents

Abstract

The invention discloses application of a traditional Chinese medicine composition in preparation of a drug used for resisting the influenza A (H3N2) virus. The traditional Chinese medicine composition is composed of fructus forsythia, honeysuckle flower, ephedra stem, bitter apricot kernel and the like, has wide-spectrum antiviral effect and can effectively kill viruses, bring down a fever and diminish inflammation; and experiments prove that the traditional Chinese medicine composition is definitely capable of resisting the influenza A (H3N2) virus.

Description

The application of a kind of Chinese medicine composition in the medicine of the anti-A type H3N2 influenza virus of preparation

Technical field

The present invention relates to a kind of novelty teabag of Chinese medicine composition, particularly, relate to the application of a kind of Chinese medicine composition in the medicine of the anti-A type H3N2 influenza virus of preparation, belong to application in TCM field.

Background technology

Influenza A virus H3N2 hypotype, it is the one of influenza A virus, it is the respiratory system disease caused by myxovirus, can by the respiratory infectious of many animals, patient's multilist reveals the symptom of common influenza, occur diarrhoea and vomiting sometimes, severe one can secondary pneumonia and respiratory failure, even death.

At present, prevention A type H3N2 influenza virus, mainly vaccination; For treatment influenza virus also not effective especially medicine, mainly recommend oseltamivir phosphate, trade name oseltamivir phosphate capsule, another name tamiflu, Tamiflu, every capsules is containing 98.5 milligrams of oseltamivir phosphates (being equivalent to the Oseltamivir of 75 milligrams).Company of Roche Group of Switzerland produces, and within 1999, in U.S.'s listing, the second half of 2002 is in Discussion on Chinese Listed.Oseltamivir phosphate capsule is the activity by suppressing neuraminidase, plays and suppresses virus at human body internal diffusion, be included in the effect of respiratory system diffusion, thus break viral infection cyclic process.When neuraminidase is suppressed, virus just cannot walk out the cell protecting them, and dies subsequently, again cannot spread and infect other cell of human body.Neuraminidase all exists on A type, Influenza B virus, so the case that Oseltamivir infects A type and Influenza B virus is all effective, medicine substantially reduce the number the virus of disseminating out by sneeze, but can not be prevented completely.

Summary of the invention

The present invention relates to a kind of novelty teabag of Chinese medicine composition, particularly, relate to the application of a kind of Chinese medicine composition in the medicine of the anti-A type H3N2 influenza virus of preparation.

Chinese medicine of the present invention can be had the Chinese medicine of same or similar effect to replace, and these medical materials all can be concocted according to " national Chinese medicine processing specification " or " Chinese medicine voluminous dictionary ".

The object of the invention is to provide the application of a kind of Chinese medicine composition in the medicine of the anti-A type H3N2 influenza virus of preparation treatment, and described Chinese medicine composition is made up of the crude drug of following weight portion:

Fructus Forsythiae 240-280 Flos Lonicerae 240-280 Radix Isatidis 240-280 Radix Et Rhizoma Rhei 45-55

Herba Pogostemonis 70-90 Rhizoma Dryopteris Crassirhizomatis 240-280 Radix Rhodiolae 70-90 Mentholum 6-8

Herba Ephedrae 70-90 Semen Armeniacae Amarum 70-90 Herba Houttuyniae 240-280 Radix Glycyrrhizae 70-90

Gypsum Fibrosum 240-280.

Preferably, this Chinese medicine composition is made up of the crude drug of following weight portion:

Fructus Forsythiae 240 Flos Lonicerae 280 Radix Isatidis 240 Radix Et Rhizoma Rhei 55 Herba Pogostemonis 70

Rhizoma Dryopteris Crassirhizomatis 280 Radix Rhodiolae 90 Mentholum 8 Herba Ephedrae 70 Semen Armeniacae Amarum 90

Herba Houttuyniae 240 Radix Glycyrrhizae 90 Gypsum Fibrosum 240.

Or:

Fructus Forsythiae 280 Flos Lonicerae 240 Radix Isatidis 280 Radix Et Rhizoma Rhei 45 Herba Pogostemonis 90

Rhizoma Dryopteris Crassirhizomatis 240 Radix Rhodiolae 70 Mentholum 6 Herba Ephedrae 90 Semen Armeniacae Amarum 70

Herba Houttuyniae 280 Radix Glycyrrhizae 70 Gypsum Fibrosum 280.

Or:

Fructus Forsythiae 255 Flos Lonicerae 255 Radix Isatidis 255 Radix Et Rhizoma Rhei 51 Herba Pogostemonis 85

Rhizoma Dryopteris Crassirhizomatis 255 Radix Rhodiolae 85 Mentholum 7.5 Herba Ephedrae 85 Semen Armeniacae Amarum 85

Herba Houttuyniae 255 Radix Glycyrrhizae 85 Gypsum Fibrosum 255.

Preferentially, in the raw materials used medicine of described Chinese medicine composition, Herba Ephedrae is Herba ephedrae, and Semen Armeniacae Amarum is Semen Armeniacae Amarum (parched).

The active component that present invention also offers described Chinese medicine composition is made up of following steps:

(1) take Chinese crude drug according to crude drug part by weight, clean;

(2) Herba Pogostemonis is cataclasm, adds 8-10 times amount water extraction volatile oil, carries the 4 hours time of oil, collects volatile oil, for subsequent use; After extracting liquid filtering, residue discards, and filtrate is for subsequent use;

(3) Fructus Forsythiae, Herba Ephedrae, Herba Houttuyniae, Radix Et Rhizoma Rhei, with the ethanol extraction 2 times of 6-10 times amount 50-90%, each 1-3 hour, extracting solution merges and filters, and reclaim ethanol, filtrate is for subsequent use;

(4) Flos Lonicerae, Gypsum Fibrosum, Radix Isatidis, Rhizoma Dryopteris Crassirhizomatis, Radix Glycyrrhizae, Radix Rhodiolae, adding 7-11 times amount soak by water to boiling, adding Semen Armeniacae Amarum, decoct 2 times, each 0.5-2.5 hour, extracting solution merges filtration, filtrate after oil carried by gained filtrate and step (2) Herba Pogostemonis merges, be condensed into 60 DEG C time measure relative density be the clear paste of 1.10-1.15, add ethanol, reconcile to determining alcohol be 70%, cold preservation is placed, filter, reclaim ethanol to without alcohol taste, obtain clear paste for subsequent use;

(5) step (4) gained gained clear paste and step (3) gained alcohol extract are merged, being concentrated into and measuring relative density 60 DEG C time is the clear paste of 1.15-1.20, and dry, get dry extract powder, for subsequent use;

Step (5) gained dried cream powder, step (2) gained volatile oil and Mentholum form the active component of this Chinese medicine composition jointly.

The dosage form of medicine of the present invention is capsule, tablet, powder, oral liquid, pill, tincture, syrup, suppository, gel, spray or injection.

For enabling above-mentioned dosage form realize, the acceptable adjuvant of pharmacy need be added when preparing these dosage forms, such as: filler, disintegrating agent, lubricant, suspending agent, binding agent, sweeting agent, correctives, antiseptic, substrate etc.Filler comprises: starch, pregelatinized Starch, lactose, mannitol, chitin, microcrystalline Cellulose, sucrose etc.; Disintegrating agent comprises: starch, pregelatinized Starch, microcrystalline Cellulose, carboxymethyl starch sodium, crospolyvinylpyrrolidone, low-substituted hydroxypropyl cellulose, cross-linking sodium carboxymethyl cellulose etc.; Lubricant comprises: magnesium stearate, sodium lauryl sulphate, Pulvis Talci, silicon dioxide etc.; Suspending agent comprises: polyvinylpyrrolidone, microcrystalline Cellulose, sucrose, agar, hydroxypropyl emthylcellulose etc.; Binding agent comprises, starch slurry, polyvinylpyrrolidone, hydroxypropyl emthylcellulose etc.; Sweeting agent comprises: saccharin sodium, Aspartane, sucrose, cyclamate, enoxolone etc.; Correctives comprises: sweeting agent and various essence; Antiseptic comprises: parabens, benzoic acid, sodium benzoate, sorbic acid and its esters, benzalkonium bromide, acetic acid chloroethene are fixed, Folium eucalypti globueli (Eucalyptus globulus Labill.) wet goods; Substrate comprises: PEG6000, PEG4000, insect wax etc. (Fan Biting " pharmacy of Chinese materia medica ", Shanghai Science Press. December the 1st edition in 1997. the adjuvant that each dosage form is recorded).

The wherein preparation method of capsule is made up of following steps:

(1) take Chinese crude drug according to crude drug part by weight, clean;

(2) Herba Pogostemonis is cataclasm, adds 5-8 times amount water extraction volatile oil, carries the 4 hours time of oil, collects volatile oil, for subsequent use; After extracting liquid filtering, residue discards, and filtrate is for subsequent use;

(3) Fructus Forsythiae, Herba Ephedrae, Herba Houttuyniae, Radix Et Rhizoma Rhei, with the ethanol extraction 2 times of 6-10 times amount 50-90%, each 1-3 hour, extracting solution merges and filters, and reclaim ethanol, filtrate is for subsequent use;

(4) Flos Lonicerae, Gypsum Fibrosum, Radix Isatidis, Rhizoma Dryopteris Crassirhizomatis, Radix Glycyrrhizae, Radix Rhodiolae, adding 7-11 times amount soak by water to boiling, adding Semen Armeniacae Amarum, decoct 2 times, each 0.5-2.5 hour, extracting solution merges filtration, filtrate after oil carried by gained filtrate and step (2) Herba Pogostemonis merges, be condensed into 60 DEG C time measure relative density be the clear paste of 1.10-1.15, add ethanol, reconcile to determining alcohol be 70%, cold preservation is placed, filter, reclaim ethanol to without alcohol taste, obtain clear paste for subsequent use;

(5) step (4) gained gained clear paste and step (3) gained alcohol extract are merged, being concentrated into and measuring relative density 60 DEG C time is the clear paste of 1.15-1.20, and dry, get dry extract powder, for subsequent use;

(6) step (5) gained dried cream powder is added suitable pharmaceutically acceptable adjuvant to granulate;

(7) Mentholum, step (2) gained volatile oil are added dissolve with ethanol, spray into step (6) gained granule, airtight, mixing, incapsulates, to obtain final product.

The wherein preparation method of granule is made up of following steps:

(1) take Chinese crude drug according to crude drug part by weight, clean, cataclasm as one sees fit;

(2) Herba Pogostemonis is cataclasm, adds 5-8 times amount water extraction volatile oil, carries the 4 hours time of oil, collects volatile oil, for subsequent use; After extracting liquid filtering, residue discards, and filtrate is for subsequent use;

(3) Fructus Forsythiae, Herba Ephedrae, Herba Houttuyniae, Radix Et Rhizoma Rhei, with the ethanol extraction 2 times of 6-10 times amount 50-90%, each 1-3 hour, extracting solution merges and filters, and reclaim ethanol, filtrate is for subsequent use;

(4) Flos Lonicerae, Gypsum Fibrosum, Radix Isatidis, Rhizoma Dryopteris Crassirhizomatis, Radix Glycyrrhizae, Radix Rhodiolae, adding 7-11 times amount soak by water to boiling, adding Semen Armeniacae Amarum, decoct 2 times, each 0.5-2.5 hour, extracting solution merges filtration, filtrate after oil carried by gained filtrate and step (2) Herba Pogostemonis merges, be condensed into 60 DEG C time measure relative density be the clear paste of 1.10-1.15, add ethanol, reconcile to determining alcohol be 70%, cold preservation is placed, filter, reclaim ethanol to without alcohol taste, obtain clear paste for subsequent use;

(5) step (4) gained gained clear paste and step (3) gained alcohol extract are merged, being concentrated into and measuring relative density 60 DEG C time is the clear paste of 1.15-1.20, and dry, get dry extract powder, for subsequent use;

(6) step (5) gained dried cream powder is added suitable pharmaceutically acceptable adjuvant to granulate;

(7) Mentholum, step (2) gained volatile oil are added dissolve with ethanol, spray into step (6) gained granule, airtight, mixing, pack, to obtain final product.

The preparation method of preferred granule is:

(1) take Chinese crude drug according to crude drug part by weight, clean, cataclasm as one sees fit;

(2) Herba Pogostemonis is cataclasm, adds 6 times amount water extraction volatile oil, carries the 4 hours time of oil, collects volatile oil, for subsequent use; After extracting liquid filtering, residue discards, and filtrate is for subsequent use;

(3) Fructus Forsythiae, Herba Ephedrae, Herba Houttuyniae, Radix Et Rhizoma Rhei, by the ethanol extraction 2 times of 8 times amount 70%, 2 hours first times, second time 1.5 hours, extracting solution merges and filters, and reclaim ethanol, filtrate is for subsequent use;

(4) Flos Lonicerae, Gypsum Fibrosum, Radix Isatidis, Rhizoma Dryopteris Crassirhizomatis, Radix Glycyrrhizae, Radix Rhodiolae, adds 9 times amount soak by water to boiling, and adds Semen Armeniacae Amarum, decocts 2 times, 1.5 hours first times, second time 1 hour, extracting solution merges filtration, filter liquor after oil carried by gained filtrate and step (2) Herba Pogostemonis merges, being condensed into and measuring relative density 60 DEG C time is the clear paste of 1.10-1.15, and add 95% ethanol, being adjusted to determining alcohol is 70%, cold preservation is placed, filter, reclaim ethanol to without alcohol taste, obtain clear paste filtrate;

(5) step (4) gained clear paste filtrate and step (3) gained alcohol extract are merged, being concentrated into and measuring relative density 60 DEG C time is the clear paste of 1.15-1.20, for subsequent use;

(6) step (5) gained clear paste is added suitable pharmaceutically acceptable adjuvant to granulate;

(7) Mentholum, step (2) gained volatile oil are added dissolve with ethanol, spray into step (6) gained granule, airtight, mixing, pack, to obtain final product.

Other dosage forms of medicine of the present invention are in proportion after weighting raw materials, adopt conventional preparation method preparation, such as, the preparation technology that Fan Biting " pharmacy of Chinese materia medica " (Shanghai Science Press 1997 December the 1st edition) records, makes the acceptable regular dosage form of pharmaceutics.

Pharmaceutical composition of the present invention confirms by experiment, and the compositions only within the scope of each flavour of a drug of the present invention all has clear and definite antivirus action to A type H3N2 influenza virus, surpasses compositions not this effect formed outside going beyond the scope.

Experimental example:

For the curative effect of the anti-A type H3N2 influenza virus of proved invention medicine, with the capsule (hereinafter referred to as medicine of the present invention) obtained by embodiment 1, carry out following research:

Part I medicine of the present invention causes the research of the improvement result of injury of lung to mouse infection H3N2 influenza virus:

1. experiment material

1.1 medicine medicine of the present invention (LH) (lot number: 20120628) and the side of tearing open (comprise MAXINGSHIGAN TANG (MXSGTT, Ma Xing Shi Gan Tang), Lonicerae and Forsythiae Powder (YP, Yinqiao Powder), exceed compositions (see embodiment 4) and the Radix Et Rhizoma Rhei (RO of medicine scope of the present invention, Rheum Officinale)) be dried cream powder, effluent north Shijiazhuang Yiling Pharmaceutical Co., Ltd provides.Positive control agents Ribavirin available from Sigma (St. Louis, MO).

1.2 animal SPF level BALB/C mice, body weight 14 ~ 15g, purchased from Guangdong Province's Experimental Animal Center (credit number: SCXK(Guangdong) 2008-0002).

1.3 cells and Strain Testis et Pentis Canis epithelial cell (MDCK) draw from American classic culture collecting center (ATCC, American Type Culture Collection), adopt the DMEM(Dulbecco's Modi ed Eagle's Medium containing 10% hyclone and 1% mycillin) culture fluid cultivates.A type H3N2 influenza virus A ichi strain (A/Aichi/2/68, H3N2) purchased from ATCC, with virus infected mice, collect lung tissue suspension repeatedly to go down to posterity acquisition mice, inoculation 9 ~ 11 age in days chick embryo allantoic cavities before using, cultivate 2 days for 37 DEG C, collect allantoic fluid for subsequent use.According to the method for Reed-Muench at mouse assay LD50(50% fatal dose).

2. experimental technique

2.1 animal groupings

Mice is divided into 8 groups at random, comprise Normal group, virus control group, ribavirin 75mg/kg/ days dosage group, LH 1300 and 650mg/kg/ days dosage groups, MXSGT 120mg/kg/ days dosage groups, YP 490mg/kg/ days dosage groups, contrast groups (exceeding the compositions of medicine scope of the present invention) 1200mg/kg/ days dosage group and RO 30mg/kg/ days dosage groups, often organize 10, be female.

2.2 Lung Exponent and Pneumovirinae titer determination

Each group of mice is under ether light anesthesia, and with the H3N2 influenza virus H3N2 allantoic fluid collunarium infecting mouse of 5 LD50, every only 50 μ L, Normal group instills physiological saline solution with method.Mouse infection virus is after 2 hours, and medicine group every gastric infusion 0.2mL, Normal group and virus control group give 0.2mL normal saline, every day 1 time, successive administration 5 days.

Infect latter 5th day, often group anesthesia execution 3 mices, solution takes lung, be that the DMEM of 1:10 carries out homogenate to lung tissue with w/v, centrifugal 10 minutes of 10000 rpm/min, collect supernatant, adopt TCID50 method to measure virus titer on mdck cell, calculate TCID50 value with Reed-Muench method.

Often organize the anesthesia in the 7th day after infection of remaining mice to put to death, anatomic observation pulmonary lesion, and weigh in, lung weight, measure its Lung Exponent (Lung Exponent=lung heavy (g)/body weight (g) × 100).

2.3 pathologic inspections

After above-mentioned lung is weighed, fix with 4% paraformaldehyde, routine drawn materials, dewater, paraffin embedding, section (thick about 4 ~ 6 μm), with hematoxylin-eosin (HE) dyeing, the change of light Microscopic observation lung tissue.

2.4 statistical procedures

Experimental data with ± SD represents, Lung Exponent and Pneumovirinae titre adopt variance analysis t inspection, and p < 0.05 is significant difference, and p < 0.01 and p < 0.001 is significant differences.

3. result

3.1 medicines increase mouse lung index and the inhibitory action of lung tissue virus multiplication

H3N2 influenza infection mice is after 7 days, and the Lung Exponent of Normal group and ribavirin group compares with virus control group, has significant differences (p < 0.001), shows modeling success (table 1).The Lung Exponent caused after 1300 and the 650mg/kg/ days dosage groups of LH can reduce mouse infection virus increases, compared with virus control group, there is significant differences (p < 0.01) (table 1), the difference of other medicines group Lung Exponent and virus control group is all not obvious, not statistically significant (p > 0.05) (table 1).In addition, the lung tissue virus titer of 1300 and the 650mg/kg/ days dosage group mices of LH is also significantly declined (p < 0.05), and other medicines group to the virus multiplication of mouse lung tissue without obvious inhibitory action (p > 0.05) (table 1).

Table 1 medicine is on the impact of H3N2 influenza infection mouse lung exponential sum lung tissue virus multiplication

Compare with virus control group, * p ﹤ 0.05, * * p ﹤ 0.01, * * * p ﹤ 0.001.

3.2 drug on viral infecting mouses cause the improvement result of pneumonia

Each group of mouse lung tissue pathological change result as shown in Figure 1, Normal group (Fig. 1-A) mouse lung tissue alveolar wall structural integrity, alveolar normal in size, without inflammatory cell infiltration; In virus control group (Fig. 1-B), pathologic change shows as significant bronchus and interstitial pneumonia, adjoint lymphocyte and monocyte infiltration widely.Ribavirin positive controls (Fig. 1-C) shows alveolar wall and thickens not obvious, and inflammatory reaction is comparatively light, and inflammatory exudate is less.Compared with viral group, 1300 and the 650mg/kg/ days dosage group lesions visible range shorter of LH, lesion degree alleviates to some extent, but still visible pulmonary branches trachea and interstitial pneumonia (Fig. 1-D, E).Other medicines group extent of disease and degree be virus control group no significant difference (Fig. 1-F ~ I) all comparatively.

4. conclusion

Medicine of the present invention can alleviate H3N2 influenza virus A ichi strain (A/Aichi/2/68, H3N2) and infect the increase of mouse lung index and inflammatory pulmonary damage that cause, and effectively suppresses lung tissue virus multiplication.

The protective effect that Part II medicine of the present invention is died to H3N2 influenza infection mouse lethal

1. experiment material

1.1 medicine

See Part I 1.1.

1.2 animal

See Part I 1.2.

1.3 Strain

See Part I 1.3.

2. experimental technique

2.1 animal groupings

See Part I 2.1.

The lethal protective effect of dying of 2.2 drug on viral infecting mouse

Each group of mice collunarium infects the influenza virus liquid of 5 LD50, and every only 50 μ L, normal control instills physiological saline solution with method.Infect latter 2 hours every gastric infusion 0.2mL, every day 1 time, continuous 5 days.Normal group and virus control group pour into normal saline with method, observe mouse invasion symptom, body weight, time-to-live and survival number day by day, Continuous Observation 15 days.To infect in 24 hours and dead be considered as unusual death.Calculating mean survival time (my god) and mortality risk ratio.

3. statistical procedures

This part experimental data statistics adopts Graphpad software.Describe survival curve with Kaplan-Meier method, analyze with Log-Rank Test (log-rank).Mean survival time adopts variance analysis t inspection.Mortality risk ratio (Hazard ratio) adopts Manel-Haenzel method to calculate, significance analysis is carried out by Gehan-Breslow-Wilcoxin, p < 0.05 is significant difference, and p < 0.01 and p < 0.001 is significant differences.

4. result

After virus the 2nd day, mice started to occur weight loss virus control group, and hair is in disorder, become thin and the phenomenon such as poor appetite, then developed into rapid breathing gradually, and ventral breathing and uniting such as to roll up at the symptom, and survival rate is 20%(table 2).Ribavirin group mice experiences of short duration weight loss, and after infecting, the 5th day body weight is gone up gradually, and survival rate is 100%(p<0.001) (table 2).Within 2nd day, there is weight loss after infection in other medicines treatment group mice; bottom out in 7th day; survival rate is 10% ~ 30%(p> 0.05), show medicine of the present invention and the side of tearing open to mouse infection influenza virus without dead protective effect (table 2).But, 1300 and the 650 mg/kg/ days dosage group of LH can the survival natural law (p<0.05) (table 2) of significant prolongation mice, and wherein 1300 mg/kg/ days dosage groups also significantly reduce dead mouse risk (p<0.05) (table 2).

Table 2 medicine is to the dead protective effect a infecting H3N2 influenza virus mice

^a compare with virus control group, * p﹤ 0.05, * * p﹤ 0.01, * * * p﹤ 0.001.

^b infect the Average Survival natural law of mice dead in latter 15 days.

 

5. conclusion

Medicine of the present invention can extend the Average Survival natural law of H3N2 influenza virus A ichi strain (A/Aichi/2/68, H3N2) infecting mouse, and reduces its mortality risk.

Accompanying drawing explanation

(A) Fig. 1 is the pathologic finding figure of Mus lung after Drug therapy, (A) normal control, (B) virus control, (C) ribavirin (75mg/kg/ days), (D) LH(1300mg/kg/ days), (E) LH (650mg/kg/ days), (F) MXSGT(120mg/kg/ days), (G) YP(490mg/kg/ days), (H) contrast groups (1200mg/kg/ days), (I) RO(30mg/kg/ days);

Fig. 2 is mice average weight every day variation diagram.

Detailed description of the invention

Embodiment 1:

Crude drug formula is:

Fructus Forsythiae 255 g Flos Lonicerae 255 g Radix Isatidis 255 g Radix Et Rhizoma Rhei 51 g Herba Pogostemonis 85 g

Rhizoma Dryopteris Crassirhizomatis 255 g Radix Rhodiolae 85 g Mentholum 7.5 g Herba ephedrae 85 g

Semen Armeniacae Amarum (parched) 85 g Herba Houttuyniae 255 g Radix Glycyrrhizae 85 g Gypsum Fibrosum 255 g

Preparation method is:

(1) take Chinese crude drug according to above-mentioned prescription, clean, cataclasm as one sees fit;

(2) Herba Pogostemonis is cataclasm, adds 6 times amount water extraction volatile oil, carries the 4 hours time of oil, collects volatile oil, for subsequent use; After extracting liquid filtering, residue discards, and filtrate is for subsequent use;

(3) Fructus Forsythiae, Herba Ephedrae, Herba Houttuyniae, Radix Et Rhizoma Rhei, by the ethanol extraction 2 times of 8 times amount 70%, 2 hours first times, second time 1.5 hours, extracting solution merges and filters, and reclaim ethanol, filtrate is for subsequent use;

(4) Flos Lonicerae, Gypsum Fibrosum, Radix Isatidis, Rhizoma Dryopteris Crassirhizomatis, Radix Glycyrrhizae, Radix Rhodiolae, add 9 times amount soak by water to boiling, add Semen Armeniacae Amarum and decoct 2 times, 1.5 hours first times, second time 1 hour, extracting solution merges filtration, filtrate after oil carried by gained filtrate and step (2) Herba Pogostemonis merges, being condensed into and measuring relative density 60 DEG C time is the clear paste of 1.10, add ethanol, being adjusted to determining alcohol is 70%, cold preservation places 24 hours, filter, reclaim ethanol extremely without alcohol taste, gained filtrate and step (3) gained alcohol extract merge, being concentrated into and measuring relative density 60 DEG C time is the clear paste of 1.15, spraying dry, get dry extract powder, for subsequent use,

(5) step (4) gained dried cream powder is added starch 138 grams, use 85% alcohol granulation;

(6) Mentholum, step (2) gained volatile oil are added dissolve with ethanol, spray into step (5) gained granule, airtight, mixing, loads 1000 capsules, to obtain final product.

Embodiment 2:

Crude drug formula is:

Fructus Forsythiae 280g Flos Lonicerae 240g Radix Isatidis 280g Radix Et Rhizoma Rhei 45g Herba Pogostemonis 90g Rhizoma Dryopteris Crassirhizomatis 240g Radix Rhodiolae 70g Mentholum 6g Herba Ephedrae 90g Semen Armeniacae Amarum 70g Herba Houttuyniae 280 g Radix Glycyrrhizae 70g Gypsum Fibrosum 280 g

Preparation method is:

(1) take Chinese crude drug according to above-mentioned prescription, clean, cataclasm as one sees fit;

(2) Herba Pogostemonis is cataclasm, adds 5 times amount water extraction volatile oil, carries the 4 hours time of oil, collects volatile oil, for subsequent use; After extracting liquid filtering, residue discards, and filtrate is for subsequent use;

(3) Fructus Forsythiae, Herba Ephedrae, Herba Houttuyniae, Radix Et Rhizoma Rhei, by the ethanol extraction 2 times of 6 times amount 50%, 3 hours first times, second time 1 hour, extracting solution merges and filters, and reclaim ethanol, filtrate is for subsequent use;

(4) Flos Lonicerae, Gypsum Fibrosum, Radix Isatidis, Rhizoma Dryopteris Crassirhizomatis, Radix Glycyrrhizae, Radix Rhodiolae, add 7 times amount soak by water to boiling, add Semen Armeniacae Amarum and decoct 2 times, 0.5 hour first time, second time 2.5 hours, extracting solution merges filtration, filtrate after oil carried by gained filtrate and step (2) Herba Pogostemonis merges, being condensed into and measuring relative density 60 DEG C time is the clear paste of 1.15, add ethanol, being adjusted to determining alcohol is 70%, cold preservation places 24 hours, filter, reclaim ethanol extremely without alcohol taste, gained filtrate and step (3) gained alcohol extract merge, being concentrated into and measuring relative density 60 DEG C time is the clear paste of 1.20, spraying dry, get dry extract powder, for subsequent use,

(5) step (4) gained dried cream powder is added starch 134 grams, use 85% alcohol granulation;

(6) Mentholum, step (2) gained volatile oil are added dissolve with ethanol, spray into step (5) gained granule, formulation method is made tablet and be get final product routinely.

Embodiment 3:

Crude drug formula is:

Fructus Forsythiae 240 g Flos Lonicerae 280 g Radix Isatidis 240 g Radix Et Rhizoma Rhei 55 g Herba Pogostemonis 70 g Rhizoma Dryopteris Crassirhizomatis 280 g Radix Rhodiolae 90 g Mentholum 8 g Herba Ephedrae 70 g Semen Armeniacae Amarum 90 g Herba Houttuyniae 240 g Radix Glycyrrhizae 90 g Gypsum Fibrosum 240 g.

Preparation method is:

(1) take Chinese crude drug according to above-mentioned prescription, clean, cataclasm as one sees fit;

(2) Herba Pogostemonis is cataclasm, adds 10 times amount water extraction volatile oil, carries the 4 hours time of oil, collects volatile oil, for subsequent use; After extracting liquid filtering, residue discards, and filtrate is for subsequent use;

(3) Fructus Forsythiae, Herba Ephedrae, Herba Houttuyniae, Radix Et Rhizoma Rhei, by the ethanol extraction 2 times of 10 times amount 90%, 1 hour first time, second time 3 hours, extracting solution merges and filters, and reclaim ethanol, filtrate is for subsequent use;

(4) Flos Lonicerae, Gypsum Fibrosum, Radix Isatidis, Rhizoma Dryopteris Crassirhizomatis, Radix Glycyrrhizae, Radix Rhodiolae, add 11 times amount soak by water to boiling, add Semen Armeniacae Amarum and decoct 2 times, 2.5 hours first times, second time 0.5 hour, extracting solution merges filtration, filtrate after oil carried by gained filtrate and step (2) Herba Pogostemonis merges, being condensed into and measuring relative density 60 DEG C time is the clear paste of 1.12, add ethanol, being adjusted to determining alcohol is 70%, cold preservation places 24 hours, filter, reclaim ethanol extremely without alcohol taste, gained filtrate and step (3) gained alcohol extract merge, being concentrated into and measuring relative density 60 DEG C time is the clear paste of 1.16, spraying dry, get dry extract powder, for subsequent use,

(5) Mentholum, step (2) gained volatile oil are added dissolve with ethanol, spray into step (4) gained dried cream powder, formulation method is made pill and be get final product routinely.

Embodiment 4:

Crude drug formula is:

Fructus Forsythiae 170g Flos Lonicerae 170g Herba Ephedrae (processed) 57g Semen Armeniacae Amarum (parched) 57g

Gypsum Fibrosum 170g Radix Isatidis 170g Rhizoma Dryopteris Crassirhizomatis 170g Herba Houttuyniae 170g

Herba Pogostemonis 57g Radix Et Rhizoma Rhei 34g Radix Rhodiolae 57g Mentholum 5.0g

Radix Glycyrrhizae 57g

Preparation method:

(1) extraction process:

(1) take Chinese crude drug according to above-mentioned recipe quantity, clean, cataclasm as one sees fit;

(2) Herba Pogostemonis adds 6 times amount water extraction volatile oil, and carry oil time 4h, collect volatile oil, oil yield is 0.33 ± 0.05%, and for subsequent use after extracting liquid filtering, residue discards;

(3) Fructus Forsythiae, Herba Ephedrae (processed), Herba Houttuyniae, Radix Et Rhizoma Rhei, with the ethanol extraction 2 times of 8 times amount 70%, 2 hours first times, second time 1.5 hours, extracting liquid filtering, filtrate merges, and reclaims ethanol to without alcohol taste, for subsequent use;

(4) Flos Lonicerae, Semen Armeniacae Amarum (parched), Gypsum Fibrosum, Radix Isatidis, Rhizoma Dryopteris Crassirhizomatis, Radix Glycyrrhizae, Radix Rhodiolae, add 9 times amount soak by water to boiling, add Semen Armeniacae Amarum (parched), decoct 2 times, 1.5 hours first times, second time 1 hour, extracting liquid filtering, filtrate merge add simultaneously step (2) Herba Pogostemonis carry oil after aqueous solution, being condensed into 60 DEG C of mensuration relative densities is 1.10 clear paste, add 95% ethanol, limit edged stirs, to determining alcohol 70%, cold preservation places 24 hours, filter, filtrate recycling ethanol is extremely without alcohol taste, merge with alcohol extract, being concentrated into and being condensed into 60 DEG C of mensuration relative densities is 1.20 clear paste, for subsequent use,

(2) preparation process:

(5) pharmaceutical formulation is: step (4) gained thick paste 335.5g Mentholum 5g

Step (2) gained patchouli oil 0.2ml Icing Sugar 342.5g dextrin 514.0g

(6) granulate: by Icing Sugar and dextrin mix homogeneously, make binding agent soft material with clear paste, 14 eye mesh screens are granulated, 60-65 DEG C of oven dry, 10 eye mesh screen granulate;

(7) subpackage: sift out fine powder appropriate, Mentholum, Herba Pogostemonis Volatile oil are added ethanol in proper amount, dissolves, sprays in fine powder, mix homogeneously, and mix homogeneously with granule, airtight half an hour, and pack, obtaining final product, above prescription can be made into granule 1000g.

Claims (9)

1. the application of Chinese medicine composition in the medicine of the anti-A type H3N2 influenza virus of preparation, is characterized in that being made up of the crude drug of following weight portion:

Fructus Forsythiae 240-280 Flos Lonicerae 240-280 Radix Isatidis 240-280 Radix Et Rhizoma Rhei 45-55

Herba Pogostemonis 70-90 Rhizoma Dryopteris Crassirhizomatis 240-280 Radix Rhodiolae 70-90 Mentholum 6-8

Herba Ephedrae 70-90 Semen Armeniacae Amarum 70-90 Herba Houttuyniae 240-280 Radix Glycyrrhizae 70-90

Gypsum Fibrosum 240-280.

2. application according to claim 1, is characterized in that being made up of the crude drug of following weight portion:

Fructus Forsythiae 240 Flos Lonicerae 280 Radix Isatidis 240 Radix Et Rhizoma Rhei 55 Herba Pogostemonis 70

Rhizoma Dryopteris Crassirhizomatis 280 Radix Rhodiolae 90 Mentholum 8 Herba Ephedrae 70 Semen Armeniacae Amarum 90

Herba Houttuyniae 240 Radix Glycyrrhizae 90 Gypsum Fibrosum 240.

3. application according to claim 1, is characterized in that being made up of the crude drug of following weight portion:

Fructus Forsythiae 280 Flos Lonicerae 240 Radix Isatidis 280 Radix Et Rhizoma Rhei 45 Herba Pogostemonis 90

Rhizoma Dryopteris Crassirhizomatis 240 Radix Rhodiolae 70 Mentholum 6 Herba Ephedrae 90 Semen Armeniacae Amarum 70

Herba Houttuyniae 280 Radix Glycyrrhizae 70 Gypsum Fibrosum 280.

4. application according to claim 1, is characterized in that being made up of the crude drug of following weight portion:

Fructus Forsythiae 255 Flos Lonicerae 255 Radix Isatidis 255 Radix Et Rhizoma Rhei 51 Herba Pogostemonis 85

Rhizoma Dryopteris Crassirhizomatis 255 Radix Rhodiolae 85 Mentholum 7.5 Herba Ephedrae 85 Semen Armeniacae Amarum 85

Herba Houttuyniae 255 Radix Glycyrrhizae 85 Gypsum Fibrosum 255.

5. the application according to any one of claim 1-4, is characterized in that the active component of described Chinese medicine composition is made up of following steps:

(1) take Chinese crude drug according to crude drug part by weight, clean;

(2) Herba Pogostemonis is cataclasm, adds 8-10 times amount water extraction volatile oil, carries the 4 hours time of oil, collects volatile oil, for subsequent use; After extracting liquid filtering, residue discards, and filtrate is for subsequent use;

(3) Fructus Forsythiae, Herba Ephedrae, Herba Houttuyniae, Radix Et Rhizoma Rhei, with the ethanol extraction 2 times of 6-10 times amount 50-90%, each 1-3 hour, extracting solution merges and filters, and reclaim ethanol, filtrate is for subsequent use;

(4) Flos Lonicerae, Gypsum Fibrosum, Radix Isatidis, Rhizoma Dryopteris Crassirhizomatis, Radix Glycyrrhizae, Radix Rhodiolae, adding 7-11 times amount soak by water to boiling, adding Semen Armeniacae Amarum, decoct 2 times, each 0.5-2.5 hour, extracting solution merges filtration, filtrate after oil carried by gained filtrate and step (2) Herba Pogostemonis merges, be condensed into 60 DEG C time measure relative density be the clear paste of 1.10-1.15, add ethanol, reconcile to determining alcohol be 70%, cold preservation is placed, filter, reclaim ethanol to without alcohol taste, obtain clear paste for subsequent use;

(5) step (4) gained gained clear paste and step (3) gained alcohol extract are merged, being concentrated into and measuring relative density 60 DEG C time is the clear paste of 1.15-1.20, and dry, get dry extract powder, for subsequent use;

Step (5) gained dried cream powder, step (2) gained volatile oil and Mentholum form the active component of this Chinese medicine composition jointly.

6. the application according to any one of claim 1-4, is characterized in that described pharmaceutical dosage form is capsule, tablet, powder, granule, oral liquid, pill, tincture, syrup, suppository, gel, spray or injection.

7. application according to claim 6, is characterized in that the preparation method of medicine capsule is made up of following steps:

(1) take Chinese crude drug according to crude drug part by weight, clean;

(2) Herba Pogostemonis is cataclasm, adds 5-8 times amount water extraction volatile oil, carries the 4 hours time of oil, collects volatile oil, for subsequent use; After extracting liquid filtering, residue discards, and filtrate is for subsequent use;

(3) Fructus Forsythiae, Herba Ephedrae, Herba Houttuyniae, Radix Et Rhizoma Rhei, with the ethanol extraction 2 times of 6-10 times amount 50-90%, each 1-3 hour, extracting solution merges and filters, and reclaim ethanol, filtrate is for subsequent use;

(4) Flos Lonicerae, Gypsum Fibrosum, Radix Isatidis, Rhizoma Dryopteris Crassirhizomatis, Radix Glycyrrhizae, Radix Rhodiolae, adding 7-11 times amount soak by water to boiling, adding Semen Armeniacae Amarum, decoct 2 times, each 0.5-2.5 hour, extracting solution merges filtration, filtrate after oil carried by gained filtrate and step (2) Herba Pogostemonis merges, be condensed into 60 DEG C time measure relative density be the clear paste of 1.10-1.15, add ethanol, reconcile to determining alcohol be 70%, cold preservation is placed, filter, reclaim ethanol to without alcohol taste, obtain clear paste for subsequent use;

(5) step (4) gained gained clear paste and step (3) gained alcohol extract are merged, being concentrated into and measuring relative density 60 DEG C time is the clear paste of 1.15-1.20, and dry, get dry extract powder, for subsequent use;

(6) step (5) gained dried cream powder is added suitable pharmaceutically acceptable adjuvant to granulate;

(7) Mentholum, step (2) gained volatile oil are added dissolve with ethanol, spray into step (6) gained granule, airtight, mixing, incapsulates, to obtain final product.

8. application according to claim 6, is characterized in that the preparation method of medicinal granule is made up of following steps:

(1) take Chinese crude drug according to crude drug part by weight, clean, cataclasm as one sees fit;

(2) Herba Pogostemonis is cataclasm, adds 5-8 times amount water extraction volatile oil, carries the 4 hours time of oil, collects volatile oil, for subsequent use; After extracting liquid filtering, residue discards, and filtrate is for subsequent use;

(3) Fructus Forsythiae, Herba Ephedrae, Herba Houttuyniae, Radix Et Rhizoma Rhei, with the ethanol extraction 2 times of 6-10 times amount 50-90%, each 1-3 hour, extracting solution merges and filters, and reclaim ethanol, filtrate is for subsequent use;

(4) Flos Lonicerae, Gypsum Fibrosum, Radix Isatidis, Rhizoma Dryopteris Crassirhizomatis, Radix Glycyrrhizae, Radix Rhodiolae, adding 7-11 times amount soak by water to boiling, adding Semen Armeniacae Amarum, decoct 2 times, each 0.5-2.5 hour, extracting solution merges filtration, filtrate after oil carried by gained filtrate and step (2) Herba Pogostemonis merges, be condensed into 60 DEG C time measure relative density be the clear paste of 1.10-1.15, add ethanol, reconcile to determining alcohol be 70%, cold preservation is placed, filter, reclaim ethanol to without alcohol taste, obtain clear paste for subsequent use;

(5) step (4) gained gained clear paste and step (3) gained alcohol extract are merged, being concentrated into and measuring relative density 60 DEG C time is the clear paste of 1.15-1.20, and dry, get dry extract powder, for subsequent use;

(6) step (5) gained dried cream powder is added suitable pharmaceutically acceptable adjuvant to granulate;

(7) Mentholum, step (2) gained volatile oil are added dissolve with ethanol, spray into step (6) gained granule, airtight, mixing, pack, to obtain final product.

9. application according to claim 8, is characterized in that the preparation method of medicinal granule is made up of following steps:

(1) take Chinese crude drug according to crude drug part by weight, clean, cataclasm as one sees fit;

(2) Herba Pogostemonis is cataclasm, adds 6 times amount water extraction volatile oil, carries the 4 hours time of oil, collects volatile oil, for subsequent use; After extracting liquid filtering, residue discards, and filtrate is for subsequent use;

(3) Fructus Forsythiae, Herba Ephedrae, Herba Houttuyniae, Radix Et Rhizoma Rhei, by the ethanol extraction 2 times of 8 times amount 70%, 2 hours first times, second time 1.5 hours, extracting solution merges and filters, and reclaim ethanol, filtrate is for subsequent use;

(4) Flos Lonicerae, Gypsum Fibrosum, Radix Isatidis, Rhizoma Dryopteris Crassirhizomatis, Radix Glycyrrhizae, Radix Rhodiolae, adds 9 times amount soak by water to boiling, and adds Semen Armeniacae Amarum, decocts 2 times, 1.5 hours first times, second time 1 hour, extracting solution merges filtration, filter liquor after oil carried by gained filtrate and step (2) Herba Pogostemonis merges, being condensed into and measuring relative density 60 DEG C time is the clear paste of 1.10-1.15, and add 95% ethanol, being adjusted to determining alcohol is 70%, cold preservation is placed, filter, reclaim ethanol to without alcohol taste, obtain clear paste filtrate;

(5) step (4) gained clear paste filtrate and step (3) gained alcohol extract are merged, being concentrated into and measuring relative density 60 DEG C time is the clear paste of 1.15-1.20, for subsequent use;

(6) step (5) gained clear paste is added suitable pharmaceutically acceptable adjuvant to granulate;

(7) Mentholum, step (2) gained volatile oil are added dissolve with ethanol, spray into step (6) gained granule, airtight, mixing, pack, to obtain final product.