CN104823859B - Resource utilization method of the bloom blue algae in blackgum tissue culture - Google Patents
Resource utilization method of the bloom blue algae in blackgum tissue culture Download PDFInfo
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- CN104823859B CN104823859B CN201510268741.2A CN201510268741A CN104823859B CN 104823859 B CN104823859 B CN 104823859B CN 201510268741 A CN201510268741 A CN 201510268741A CN 104823859 B CN104823859 B CN 104823859B
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Abstract
The invention belongs to biological technical field, and in particular to a kind of resource utilization method of bloom blue algae in blackgum tissue culture.The method comprises the steps that (1) is just for inducing culture：First is 1/2WPM+6 BA0.3～0.8mg/L+IBA0.02～0.05mg/L+3～5% bloom blue algae algae powder+white sugar 20g/L for inducing culture；(2) subculture multiplication culture：Subculture multiplication medium is 3/4WPM+6 BA0.8～1.2mg/L+IBA0.05～0.1mg/L+3～5% bloom blue algae algae powder+white sugar 20g/L；(3) tissue culture seedling rooting：Root media is 2/5WPM+NAA0.1～0.3mg/L+IAA0.1～0.3mg/L+1～2% bloom blue algae algae powder+white sugar 15g/L；(4) domestication and transplanting of test tube seedling.The present invention establishes a cheap efficient blackgum tissue culture rapid propagation system, can shorten cycle duration 10%, and cost-effective 25%, the recycling for bloom blue algae provides a kind of disposal new method of high added value.
Description
Technical field
The present invention relates to a kind of method for plant tissue culture, and in particular to a kind of bloom blue algae is in blackgum tissue culture
Resource utilization method, belongs to biological technical field.
Background technology
Blackgum (Nyssa sylvatica) belongs to deciduous tree for Nyssaceae tupelo, up to 30 meters, the wealthy round ovum of tree crown
Shape, branch horizontal stretching, autumn has set in, and blade switchs to scarlet, extremely beautiful.This kind of original North America, heliophilous species, light, it is water-fast
Wet, cold-resistant, strong adaptability, fast-growing, antipollution, wind resistance, preferably make shade tree, shade tree, landscape tree and windbreak seeds etc., if with
Semen Ginkgo, sweetgum, Aesculus chinensis Bunge etc. plant, and can form colorful autumnal scenery.By blackgum tissue culture, not only can overcome
Defect of the existing reproduction technique as existing for seminal propagation, expands its breeding coefficient, realizes biological control, and can protect
On the basis of depositing its original merit, the quick breeding and strong sprout production of blackgum seedling are realized.
In plant tissue culture industrial seedling rearing, how to reduce culture medium expense especially plant growth regulating substance expense and be typically
The key of Plant tissue culture seedling cost is reduced, if can transform by culture medium, culture medium cost has both been reduced, test tube seedling can be promoted again
Propagation and growth, then can significantly improve the economic benefit of industrial seedling rearing.
With the development of the growth and industrial and agricultural production of population, a large amount of industrial and agricultural wastewaters and sanitary sewage enter water body, make
Water body eutrophication degree aggravates, and its result causes lake, river, planktonic algae especially cyanophyceae amount reproduction growth in pond
And there is wawter bloom.When wawter bloom occurs, the water surface is covered by thick aeruginouss water bloom.In the process that frond mortality is decomposed
In, stench, destruction landscape are not only distributed, while drinking water of the various Algae toxins discharged after frustule rupture to humans and animals
Safety constitutes a serious threat.Cyanophyceae can efficient absorption and dissolve substantial amounts of nitrogen, phosphorus and organic pollution in water body, directly by its dozen
It is to remove the nutrients such as water body nitrogen phosphorus, reduce water body eutrophication degree and protect water ecological setting most straight to drag for the removal water surface
Connect, most safe, maximally effective emergent and administering method, but if the cyanophyceae after salvaging timely and effectively cannot be disposed, with
Meaning is stacked, and not only can rot smelly generation secondary pollution because of cyanophyceae, it is also possible to as the cyanophyceae rotted makes nutrient by runoff
It is again introduced into water body.By the feasibility study utilized to blue algae resource, it is intended to search out a kind of effective cyanophyceae utilization ways,
The secondary pollution for dragging for algae not only can be reduced, which can also be made to turn waste into wealth, turned harm into good.
It is reported that Japan to blue algae resource by the use of measure be mainly using algae as organic fertilizer, its fertilizer efficiency is better than
General chemical fertilizer, nitrogen, phosphorus, potassium content are above the vegetalitas organic fertilizer such as soybean cake, Herba Astragali Melilotoidis (Herba Astragali Sinici), and without to crop and people in cyanophyceae
The harmful heavy metal of body, will not contaminated soil after use.The application contains phycocyanin, cell polysaccharide and pigment from bloom blue algae
Set out etc. abundant nutrition composition, the utilization rate of nutrient substance is improve by autoclaving, reduce the harm of cyanophycean toxin, from
And improve the tissue-culturing rapid propagation benefit of blackgum.
The content of the invention
The purpose of the present invention is to overcome the deficiencies in the prior art, there is provided a kind of money of bloom blue algae in blackgum tissue culture
Source Application way, to set up a cheap efficient blackgum tissue culture rapid propagation system.
The present invention solves the scheme of the technical problem：A kind of resource profit of bloom blue algae in blackgum tissue culture
With method, comprise the steps,
(1) just for inducing culture
Bloom blue algae algae powder is after autoclaving as composition just for inducing culture；For inducing culture it is just：
1/2WPM+6-BA 0.3～0.8mg/L+IBA, 0.02～0.05mg/L+3～5% bloom blue algae algae powder+white sugar 20g/L+ agar
5～8g/L, pH5.8；Explant is obtained from healthy and strong blackgum plant, explant is inoculated in just generation induction Jing after routine disinfection
In culture medium；Cultivation temperature is 25 ± 2 DEG C, and intensity of illumination is 1500～2000Lx, and light dark period ratio is 12h:12h；
(2) subculture multiplication culture
Composition of the bloom blue algae algae powder after autoclaving as subculture multiplication medium；Subculture multiplication medium is：
3/4WPM+6-BA 0.8～1.2mg/L+IBA, 0.05～0.1mg/L+3～5% bloom blue algae algae powder+white sugar 20g/L+ agar 5
～8g/L, pH5.8；By the stem section or stem apex aseptic inoculation of the blackgum aseptic seedling for just obtaining for inducing culture in subculture multiplication
Cultivated in culture medium, cultivation temperature is 25 ± 2 DEG C, intensity of illumination is 1500～2000Lx, and light dark period ratio is 12h:
12h；
(3) tissue culture seedling rooting
Composition of the bloom blue algae algae powder after autoclaving as root media；Root media is：2/5WPM+
NAA 0.1～0.3mg/L+IAA, 0.1～0.3mg/L+1～2% bloom blue algae algae powder+white sugar 15g/L+ 5～8g/L of agar,
pH5.8；The aseptic Multiple Buds of blackgum that enrichment culture is obtained are cut into individual plant to be inoculated on root media, cultivation temperature is
25 ± 2 DEG C, intensity of illumination is 1500～2000Lx, and light dark period ratio is 12h:12h.
(4) domestication and transplanting of test tube seedling
Blackgum rooting tube plantlet is moved to vinyl house from culturing room to place after 4～7d, bottle cap 3～4d of seedling exercising is removed,
Then blackgum seedling is taken out from bottle, seedling replanting is entered in the cultivation matrix of hole tray, overlay film 5～7d of moisturizing, therebetween often
It is ventilated, suitably adds a cover sunshade net depending on intensity of illumination, removes plastic sheeting Small plastic shed after one week completely, sprays 1000 times of liquid
Thiophanate methyl, hereafter carry out normal cultivation management.
3～5% bloom blue algae algae powder refer to addition 30～50g bloom blue algae algae powder in every liter of culture medium；1～2% wawter bloom
Blue algae powder refers to addition 10～20g bloom blue algae algae powder in every liter of culture medium.Bloom blue algae is gathered from Kunming Dianchi lake, with No. 25
Plankton net harvests Dian Chi bloom blue algae, dries acquisition bloom blue algae algae powder.
Explant in the step (1) is blackgum band axillary bud semi-lignified stem section.
After in the step (4), blackgum rooting tube plantlet is taken out from bottle, first with 0.1% carbendazim to its root at
Reason 10s, then seedling replanting is entered in the cultivation matrix of hole tray.
In the step (4), ventilated frequency is for twice daily, every time 10～15min.
The present invention establishes a cheap efficient blackgum tissue culture rapid propagation system, can shorten cycle duration 10%, section
About cost 25%, the recycling for bloom blue algae provide a kind of disposal new method of high added value.
Specific embodiment
Embodiment 1
A kind of resource utilization method of bloom blue algae in blackgum tissue culture, comprises the steps,
1. just for inducing culture
Bloom blue algae algae powder is after autoclaving as composition just for inducing culture；For inducing culture it is just：
1/2WPM+6-BA 0.3～0.8mg/L+IBA, 0.02～0.05mg/L+3～5% bloom blue algae algae powder+white sugar 20g/L+ agar
5～8g/L, pH5.8；Blackgum band axillary bud semi-lignified stem section explant, explant are obtained from healthy and strong blackgum plant
It is inoculated in Jing after routine disinfection just on inducing culture；Cultivation temperature is 25 ± 2 DEG C, and intensity of illumination is 1500～2000Lx,
Light dark period ratio is 12h:12h；
2. subculture multiplication culture
Composition of the bloom blue algae algae powder after autoclaving as subculture multiplication medium；Subculture multiplication medium is：
3/4WPM+6-BA 0.8～1.2mg/L+IBA, 0.05～0.1mg/L+3～5% bloom blue algae algae powder+white sugar 20g/L+ agar 5
～8g/L, pH5.8；By the stem section or stem apex aseptic inoculation of the blackgum aseptic seedling for just obtaining for inducing culture in subculture multiplication
Cultivated in culture medium, cultivation temperature is 25 ± 2 DEG C, intensity of illumination is 1500～2000Lx, and light dark period ratio is 12h:
12h；
3. tissue culture seedling rooting
Composition of the bloom blue algae algae powder after autoclaving as root media；Root media is：2/5WPM+
NAA 0.1～0.3mg/L+IAA, 0.1～0.3mg/L+1～2% bloom blue algae algae powder+white sugar 15g/L+ 5～8g/L of agar,
pH5.8；The aseptic Multiple Buds of blackgum that enrichment culture is obtained are cut into individual plant to be inoculated on root media, cultivation temperature is
25 ± 2 DEG C, intensity of illumination is 1500～2000Lx, and light dark period ratio is 12h:12h；
4. the domestication and transplanting of test tube seedling
Blackgum rooting tube plantlet is moved to vinyl house from culturing room to place after 4～7d, bottle cap 3～4d of seedling exercising is removed,
Then blackgum seedling is taken out from bottle, gently clean culture medium with water, blackgum seedling root to be planted is used into 0.1%
After carbendazim processes 10s, seedling replanting is entered in the cultivation matrix of hole tray, overlay film 5～7d of moisturizing, therebetween daily ventilated 10
～15min twice, suitably adds a cover sunshade net depending on intensity of illumination, removes plastic sheeting Small plastic shed completely, spray 1000 times after one week
The thiophanate methyl of liquid, hereafter carries out normal cultivation management.
The present invention establishes a cheap efficient blackgum tissue culture rapid propagation system, can shorten cycle duration 10%, section
About cost 25%.
Claims (3)
1. resource utilization method of a kind of bloom blue algae in blackgum tissue culture, it is characterised in that：Comprise the steps,
(1) just for inducing culture
Bloom blue algae algae powder is after autoclaving as composition just for inducing culture；For inducing culture it is just：1/
2WPM+6-BA 0.3～0.8mg/L+IBA, 0.02～0.05mg/L+3～5% bloom blue algae algae powder+white sugar 20g/L+ agar 5
～8g/L, pH5.8；Explant is obtained from healthy and strong blackgum plant, explant is blackgum band axillary bud semi-lignified stem
Section, explant are inoculated in Jing after routine disinfection just on inducing culture；Cultivation temperature is 25 ± 2 DEG C, and intensity of illumination is 1500
～2000Lx, light dark period ratio are 12h:12h；
(2) subculture multiplication culture
Composition of the bloom blue algae algae powder after autoclaving as subculture multiplication medium；Subculture multiplication medium is：3/
4WPM+6-BA 0.8～1.2mg/L+IBA, 0.05～0.1mg/L+3～5% bloom blue algae algae powder+white sugar 20g/L+ agar 5～
8g/L, pH5.8；The stem section or stem apex aseptic inoculation of the blackgum aseptic seedling for just obtaining for inducing culture are trained in subculture multiplication
Cultivated on foster base, cultivation temperature is 25 ± 2 DEG C, intensity of illumination is 1500～2000Lx, and light dark period ratio is 12h:12h；
(3) tissue culture seedling rooting
Composition of the bloom blue algae algae powder after autoclaving as root media；Root media is：2/5WPM+NAA
0.1～0.3mg/L+IAA, 0.1～0.3mg/L+1～2% bloom blue algae algae powder+white sugar 15g/L+ 5～8g/L of agar, pH5.8；
The aseptic Multiple Buds of blackgum that enrichment culture is obtained are cut into individual plant to be inoculated on root media, cultivation temperature is 25 ± 2
DEG C, intensity of illumination is 1500～2000Lx, and light dark period ratio is 12h:12h；
(4) domestication and transplanting of test tube seedling
Blackgum rooting tube plantlet is moved to vinyl house from culturing room to place after 4～7d, bottle cap 3～4d of seedling exercising is removed, then
Blackgum seedling is taken out from bottle, seedling replanting is entered in the cultivation matrix of hole tray, overlay film 5～7d of moisturizing, it is logical daily therebetween
Wind is breathed freely, and suitably adds a cover sunshade net depending on intensity of illumination, removes plastic sheeting Small plastic shed completely, spray the first of 1000 times of liquid after one week
Base thiophanate, hereafter carries out normal cultivation management.
2. resource utilization method of the bloom blue algae as claimed in claim 1 in blackgum tissue culture, it is characterised in that：Institute
State after blackgum rooting tube plantlet in step (4) taken out from bottle, 10s is processed to its root with 0.1% carbendazim first, then will
Seedling replanting enters in the cultivation matrix of hole tray.
3. resource utilization method of the bloom blue algae as claimed in claim 1 in blackgum tissue culture, it is characterised in that：Institute
Ventilated frequency in step (4) is stated for twice daily, every time 10～15min.
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| CN103155799A (en) * | 2011-12-16 | 2013-06-19 | 詹文圆 | Method for producing high-quality needle mushroom culture medium by using blue-green algae, corncobs and broad leaf tree sawdust to serve as main raw materials |
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| JP4532706B2 (en) * | 2000-09-08 | 2010-08-25 | 花王株式会社 | Cosmetics |
| US7528232B2 (en) * | 2005-05-20 | 2009-05-05 | The University Of Kentucky Research Foundation | Utility of phylloplanins as antibiotics, selective fungicides and for enhancing microbial resistance in crop plants |
| CN104016768B (en) * | 2014-05-07 | 2016-03-30 | 合肥福泉现代农业科技有限公司 | A kind of corn cob is mushroom culture medium of raw material and preparation method thereof |
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| CN103155799A (en) * | 2011-12-16 | 2013-06-19 | 詹文圆 | Method for producing high-quality needle mushroom culture medium by using blue-green algae, corncobs and broad leaf tree sawdust to serve as main raw materials |
| CN103155802A (en) * | 2011-12-16 | 2013-06-19 | 詹文圆 | Method for producing high-quality straw rotting edible mushroom concentration culture medium by using detoxification blue-green algae dry powder and super absorbent resin to serve as main raw materials |
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