CN104800881B - Biological fiber film and its preparing method - Google Patents
Biological fiber film and its preparing method Download PDFInfo
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- CN104800881B CN104800881B CN201510046087.0A CN201510046087A CN104800881B CN 104800881 B CN104800881 B CN 104800881B CN 201510046087 A CN201510046087 A CN 201510046087A CN 104800881 B CN104800881 B CN 104800881B
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- membrane
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- biological fibers
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- 239000000835 fiber Substances 0.000 title claims abstract description 113
- 238000000034 method Methods 0.000 title description 3
- 239000012528 membrane Substances 0.000 claims abstract description 64
- 239000010410 layer Substances 0.000 claims abstract description 32
- 241000894006 Bacteria Species 0.000 claims abstract description 8
- 241000032681 Gluconacetobacter Species 0.000 claims abstract description 8
- 239000000203 mixture Substances 0.000 claims description 14
- 239000003814 drug Substances 0.000 claims description 12
- 239000011229 interlayer Substances 0.000 claims description 11
- 235000015097 nutrients Nutrition 0.000 claims description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 8
- 239000001888 Peptone Substances 0.000 claims description 6
- 108010080698 Peptones Proteins 0.000 claims description 6
- 235000019319 peptone Nutrition 0.000 claims description 6
- 108090000790 Enzymes Proteins 0.000 claims description 5
- 102000004190 Enzymes Human genes 0.000 claims description 5
- 239000003102 growth factor Substances 0.000 claims description 5
- 229920001817 Agar Polymers 0.000 claims description 3
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 claims description 3
- 229930195725 Mannitol Natural products 0.000 claims description 3
- 239000008272 agar Substances 0.000 claims description 3
- 239000000594 mannitol Substances 0.000 claims description 3
- 235000010355 mannitol Nutrition 0.000 claims description 3
- 230000002087 whitening effect Effects 0.000 claims description 3
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims 1
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 claims 1
- 210000000988 bone and bone Anatomy 0.000 claims 1
- 238000002360 preparation method Methods 0.000 abstract description 7
- 230000014759 maintenance of location Effects 0.000 abstract description 2
- 239000002344 surface layer Substances 0.000 abstract 2
- 239000004480 active ingredient Substances 0.000 abstract 1
- 230000001815 facial effect Effects 0.000 description 12
- 230000000694 effects Effects 0.000 description 9
- 238000012360 testing method Methods 0.000 description 9
- 229940041514 candida albicans extract Drugs 0.000 description 5
- 239000004744 fabric Substances 0.000 description 5
- 239000007788 liquid Substances 0.000 description 5
- 239000012138 yeast extract Substances 0.000 description 5
- 206010052428 Wound Diseases 0.000 description 4
- 208000027418 Wounds and injury Diseases 0.000 description 4
- 230000003321 amplification Effects 0.000 description 4
- 239000004745 nonwoven fabric Substances 0.000 description 4
- 238000003199 nucleic acid amplification method Methods 0.000 description 4
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 3
- 229910052799 carbon Inorganic materials 0.000 description 3
- 239000006071 cream Substances 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 239000003292 glue Substances 0.000 description 3
- 238000012423 maintenance Methods 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 230000035699 permeability Effects 0.000 description 3
- 229920000742 Cotton Polymers 0.000 description 2
- 238000005266 casting Methods 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 229910052500 inorganic mineral Inorganic materials 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 239000011707 mineral Substances 0.000 description 2
- 206010013786 Dry skin Diseases 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 206010048038 Wound infection Diseases 0.000 description 1
- 235000010489 acacia gum Nutrition 0.000 description 1
- 239000001785 acacia senegal l. willd gum Substances 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 230000003796 beauty Effects 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 230000037336 dry skin Effects 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 238000005213 imbibition Methods 0.000 description 1
- 230000008595 infiltration Effects 0.000 description 1
- 238000001764 infiltration Methods 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000003020 moisturizing effect Effects 0.000 description 1
- 239000003921 oil Substances 0.000 description 1
- 239000003755 preservative agent Substances 0.000 description 1
- 230000002335 preservative effect Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000012797 qualification Methods 0.000 description 1
- 230000001568 sexual effect Effects 0.000 description 1
- 239000002356 single layer Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 239000000341 volatile oil Substances 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- 238000004804 winding Methods 0.000 description 1
Abstract
The invention discloses a biological fiber membrane and a preparation method thereof. Wherein the biofabric membrane is formed by a bacterium of the genus gluconacetobacter and has: first and second opposing skin layers; and a three-dimensional net structure combined between the first surface layer and the second surface layer, so as to improve the moisture retention of the dressing and carry more active ingredients. The invention also provides a preparation method of the biological fiber membrane.
Description
Technical field
The present invention is related to a kind of membrane of biological fibers, in more detail, is related to a kind of biology sticked on skin fine
Tie up film.
Background technology
In current medical field, cotton pad or gauze, but such casting product antibacterial are selected in the dressing looked after in wound mostly
Property it is not good, wound infection probability is high, easily produces the phenomenon of sticky wound, and when the dressing is changed, with lacking for being difficult to remove
Point.
Replace gauze and cotton pad followed by with adhesive-bonded fabric dressing, there is preferably imbibition nothing more than the dressing of non-woven fabrics kenel
Ability and the characteristic that moist environment help wound reparation can be provided, however, when the liquid or moisture content that non-woven fabrics is drawn gradually lose
After going, the problem of wound is sticky is also easily produced.
On the other hand, modern more payes attention to cosmetology in addition to basic living demand, and the especially maintenance to face is even more
The demand emphasis of beauty industry, therefore develop a variety of mask products.The facial mask species of prior art is various, such as mud cream type face
Film, tear stripping type facial mask and demihull shape facial mask etc..
Though mud cream type facial mask is containing maintenance composition or mineral matter, having applied after facial mask needs to rinse, and maintenance composition hardly possible is by skin
It is real to absorb, and due to containing more mineral matter, and more preservative must be added to prevent bacterium in the mud cream of moistening
Growth.It is macromolecule glue, water and alcohol to tear stripping type facial mask main component, and by raising skin temperature, promotes skin blood to follow
Ring, but need to wait until that facial mask drying can just be removed due to tearing stripping type facial mask, remove process and probably sensitiveness skin is damaged, and should
It is to ask to dry and without moisturizing ingredient to tear in stripping type facial mask, for dry skin, is also not very applicable.As for demihull shape facial mask
The individual layer lamellar body of the Essence with effect is mainly adsorbed with, and various skin quality can be maintained by adjusting component, although
Apply and be not required to rinse, but do not had cleaning effect.It is made more than above-mentioned demihull shape facial mask using single-layer nonwoven.When user uses suction
When taking the non-woven fabrics of Essence, often because of considering of quickly volatilizing of moisture in non-woven fabrics, and the higher Essence of concentration,
Waste is not only produced, while the problem of can not still solving moisture evaporation.
Therefore, still a need to develop a kind of novel casting product.
The content of the invention
In view of the missing of above-mentioned known techniques, the present invention provides a kind of membrane of biological fibers, the bacterium belonged to by gluconacetobacter
Formed, and the membrane of biological fibers has：The first relative top layer and the second top layer and it is incorporated into first top layer and the second table
Space network between layer, and density of the density less than first top layer and the second top layer of the space network.
In a specific embodiment, the space network is made up of the plural biological fiber.
Furthermore, the space network has plural number key fiber parallel to each other and is interwoven in adjacent wantonly two backbone
The interlayer fiber of fiber.Key fiber and the interlayer fiber is all biological fiber, and the diameter of the key fiber is more than or equal to
The diameter of the interlayer fiber.
In still another embodiment, described membrane of biological fibers also includes active component or medicine.The active component can
For NMF, whitening composition, crease-resistant composition, exfoliating composition, growth factor (growth factors) or enzyme (enzymes).
The present invention also provides a kind of preparation method of membrane of biological fibers, including：One container for being equipped with nutrient solution is provided, wherein,
There is the nutrient solution weight point ratio to be 5:1:1 to 4:1:1 carbon source, peptone and yeast extract；And in the nutrient solution
The bacterium of middle culture gluconacetobacter category 24 to 96 hours.
The bacterium that is belonged to by gluconacetobacter of membrane of biological fibers of the present invention is formed, and the first top layer of the membrane of biological fibers and the
There is space network between two top layers, the moisture retention of the dressing is thereby lifted, and more active components can be carried.
Brief description of the drawings
Fig. 1 is the structural representation of the membrane of biological fibers of the present invention；
Fig. 2A is sweep electron microscope (SEM) photo for the space network for showing membrane of biological fibers of the present invention,
Fig. 2 B are the side view photo for showing membrane of biological fibers of the present invention；
Fig. 3 A and 3B are respectively the SEM photograph and traditional biological fiber of 500 times of amplifications of display membrane of biological fibers of the present invention
The SEM photograph of 500 times of amplifications of film；And
Fig. 4 is the testing permeability result for showing membrane of biological fibers of the present invention and commercially available traditional biological tunica fibrosa, wherein, figure
4 (a) is the test result of membrane of biological fibers of the present invention, and Fig. 4 (b) is the test result of commercially available traditional biological tunica fibrosa.
Symbol description
1 membrane of biological fibers
The top layers of 10a first
The top layers of 10b second
101 space networks
101a backbone's fibers
101b interlayer fibers
12 fibers.
Embodiment
Illustrate embodiments of the present invention below by way of particular specific embodiment, art personnel can be by this explanation
Content disclosed in book understands other advantages and effect of the present invention easily.
It should be clear that structure, ratio, size depicted in this specification institute accompanying drawings etc., only to coordinate specification to be taken off
The content shown, so that art personnel are understood with reading, is not limited to enforceable qualifications of the invention, therefore not
Has technical essential meaning, the modification of any structure, the change of proportionate relationship or the adjustment of size are not influenceing institute of the present invention
Under effect of energy generation and the purpose that can reach, it all should still fall the scope that can cover in disclosed technology contents
It is interior.Meanwhile, cited such as " first ", " second " in this specification, " on " and " one " term, it is merely convenient to the bright of narration
, and be not used to limit enforceable scope of the invention, being altered or modified for its relativeness is changing technology contents without essence
Under, when being also considered as enforceable category of the invention.
Term " parallel " herein refers to plural key fiber in same direction, such as membrane of biological fibers length direction or width
Spend the form of direction extension.
The present invention provides a kind of membrane of biological fibers, and the bacterium belonged to by gluconacetobacter is formed, and the membrane of biological fibers has：Phase
To the first top layer and the second top layer and space network, be incorporated between first top layer and the second top layer, and this is vertical
The density of body network structure is less than the density on first top layer and the second top layer.
The membrane of biological fibers of the present invention refers to by the culture of microorganism and winner.Present invention discover that being belonged to by the gluconacetobacter
Bacterium in mannitol, peptone (peptone), yeast extract (yeast extract) and the nutrient solution of agar shape
There is the biological fiber that plural stereoscopic three-dimensional winding is combined into the membrane of biological fibers side.
In the specific embodiment of production membrane of biological fibers, started by the fermented and cultured of strain, there is provided culture first
Liquid, the nutrient solution is placed in container, and nutrient solution composition include selected from animal glue, Arabic gum, agar glue, etc. known group
Point, but the nutrient solution should also have carbon source, such as mannitol, glucose or its combination, other compositions such as peptone and yeast
Extract, and the weight point ratio of carbon source, peptone and yeast extract can be 5:1:1 to 4:1:Between 1.Then control culture
The pH-value of liquid is between acidity, such as pH value 0.5 to 6, and original microorganism concentration range can be controlled in 102To 105Individual/ml,
In 25 to 28 DEG C of quiescent cultures 24 to 96 hours, due to the container of flat can be used, therefore described space network was flat
It is flat membranaceous, afterwards, treat to take out for 24 hours to 72 hours, produce the membrane of biological fibers of the present invention.
After testing, the thickness of the membrane of biological fibers is at least 20 μm, such as 20 to 30 μm or such as 20 to 26 μm or 24 to
26 μm, and the diameter of the biological fiber of membrane of biological fibers of the present invention about 20 to 100nm.Also, the per unit face of the membrane of biological fibers
Product biological fiber amount is 0.005 to 0.008g/cm2。
As shown in figure 1, the membrane of biological fibers 1 of the present invention includes relative the first top layer 10a and the second top layer 10b；And
Space network 101, is incorporated between the first top layer 10a and the second top layer 10b.
In addition, referring to Fig. 2A, it shows that the second top layer 10b is extended with space network 101, with reference in a cloth film
On fiber 12.
As illustrated, the space network 101, the fiber 12 of the cloth film is extended to from second top layer 10b, and this is vertical
Body network structure 101 is made up of plural biological fiber.Specifically, the space network 101 has plural number parallel to each other
Key fiber 101a and be interwoven in adjacent wantonly two key fiber 101a interlayer fiber 101b, in this way, in the horizontal direction
And Vertical Square connects up the adjacent wantonly two key fiber 101a, to form 3 D stereo network structure 101.And the key fiber
101a and interlayer fiber 101b is all the biological fiber, and as illustrated, the diameter of the key fiber 101a is more than or equal to this
Interlayer fiber 101b diameter.
Refer to Fig. 2 B, its show membrane of biological fibers 1 side view photo, wherein, the membrane of biological fibers 1 also have plural number that
This key fiber 101a that is parallel or extending in the length direction of membrane of biological fibers 1 or width and with the key fiber
The interlayer fiber 101b that 101a interweaves, in this embodiment, the thickness of the membrane of biological fibers 1 is 20 to 30 μm.Furthermore, such as figure institute
Show, the density of the space network is less than the density on the top layer of membrane of biological fibers 1 two, and active component or medicine are adsorbable in this
Between two top layers, the effect of Evening Primerose Oil.
In addition, film body is temporarily or permanently combined using base material in the preparation of the membrane of biological fibers, nevertheless,
The step of such a preparation method can omit the space network of the foregoing shape of overturning flat in a reservoir.For example, in Fig. 2A
Embodiment in, using cloth film as base material.
Fig. 3 A and 3B are referred to, SEM photograph and the tradition life of 500 times of amplifications of membrane of biological fibers of the present invention is respectively shown
The SEM photograph of 500 times of amplifications of fibres film.
As shown in Figure 3A, membrane of biological fibers surface of the present invention is flat, and the bar shown in figure is the cloth below film body
The fiber of film base material, therefore membrane of biological fibers surface of the present invention is very flat.But the traditional biological tunica fibrosa surface shown in Fig. 3 B is then
With many fold.Therefore, the adhesion of the membrane of biological fibers and skin is not good, so that influence sense of touch, causes medicine or work
The problem of sexual element absorbs not good.
Biological fiber amount and the water content test of the per unit area of membrane of biological fibers of the present invention
Take membrane of biological fibers of the present invention, and cut into 5cm x 5cm size after totally 16 samples, 10 points are dried in 60 DEG C
Clock, weighs dry kind of the sample of drying.With the respectively sample dry weight divided by the sample area, the per unit of the membrane of biological fibers is measured
Area biological fiber amount is 0.005 to 0.008g/cm2。
Those samples are soaked in water 5 minutes again, to weigh the weight in wet base of sample, the water content of per unit area are measured
When, calculated according to following formula：
The water content of per unit area=(weight in wet base-dry kind)/sample area
After testing, the per unit area water content of membrane of biological fibers of the present invention is 0.06 to 0.14g/cm2。
In comparison, the biological fiber amount of the per unit area of commercially available traditional biological tunica fibrosa generally only up to 0.001g/
cm2, and water content only up to 0.04g/cm2.Therefore membrane of biological fibers of the present invention can carry more moisture or active component, have
More moistening effect.
The testing permeability of membrane of biological fibers of the present invention
Membrane of biological fibers of the present invention and commercially available traditional biological tunica fibrosa are lain against on a ground respectively, then in the present invention
The dyed Essence of equivalent is added dropwise in membrane of biological fibers and commercially available traditional biological tunica fibrosa, waits 10 seconds, and visually observation should
The infiltration situation of ground.
As shown in figure 4, Fig. 4 (a) is the test result of membrane of biological fibers of the present invention, Fig. 4 (b) is commercially available traditional biological fiber
The test result of film, wherein, commercially available traditional biological tunica fibrosa is when being just added dropwise Essence, and its Essence diffusion effect is not good, etc.
Essence effectively can not be infiltrated into ground after natural after 10 seconds, but membrane of biological fibers of the present invention then has preferably elite
Liquid diffusivity and significant permeability.
In summary, membrane of biological fibers of the invention is particularly used as facial mask and used for sticking on skin, therefore the biology
Tunica fibrosa may also include active component or medicine., should by the active component or medicine input before the fermented and cultured of strain ends
On space network, the preparation of membrane of biological fibers to be done, the active component or medicine are to be included in film body, the activity
The example of composition include NMF, whitening composition, crease-resistant composition, exfoliating composition, growth factor (growth factors) or
Enzyme (enzymes).Medicine can be massage and drug, its can be paste or liquid, such as massage or relax pressure essential oil, due to the present invention
Membrane of biological fibers has space network, therefore, and active component or medicine will not be exposed easily, but the biology of the present invention is fine
After dimension film is attached on body surface or skin, applies pressure to membrane of biological fibers through manual massage or other contact devices, that is, contribute to
The release of active component or medicine, furthermore, the mode that also can pass through heating helps the release of active component or medicine.
Principle and its effect of the above-described embodiment to the illustrative present invention, not for the limitation present invention.It is any
Art personnel can modify under the spirit and scope without prejudice to the present invention to above-described embodiment.Therefore this hair
Bright rights protection scope, should be as listed by claims.
Claims (6)
1. a kind of membrane of biological fibers, the bacterium belonged to by gluconacetobacter is formed, and the membrane of biological fibers has：
Relative the first top layer and the second top layer；And
Space network, is incorporated between first top layer and the second top layer, and the density of the space network is less than this
First top layer and the density on the second top layer, wherein, the space network has plural number key fiber parallel to each other and friendship
The plural interlayer fiber of the adjacent wantonly two key fiber is woven in, key fiber and the interlayer fiber is all biological fiber, and the bone
The diameter of dry fibers is more than or equal to the diameter of the interlayer fiber, and wherein, the thickness of the membrane of biological fibers is 20 to 30 μm, should
The per unit area biological fiber amount of membrane of biological fibers is 0.005 to 0.008g/cm2, the biological fiber a diameter of 20 to
100nm, and the per unit area water content of the membrane of biological fibers is 0.06 to 0.14g/cm2。
2. membrane of biological fibers as claimed in claim 1, it is characterised in that the space network is by plural biological fiber institute structure
Into.
3. membrane of biological fibers as claimed in claim 1, it is characterised in that the key fiber is in the membrane of biological fibers length direction
Or width extension.
4. membrane of biological fibers as claimed in claim 1, in addition to active component or medicine.
5. membrane of biological fibers as claimed in claim 4, it is characterised in that the active component is NMF, whitening composition, crease-resistant
Composition, exfoliating composition, growth factor or enzyme.
6. membrane of biological fibers as claimed in claim 1, the bacterium belonged to by gluconacetobacter is with mannitol, peptone, yeast
Formed in the nutrient solution of extract and agar.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| TW103103467 | 2014-01-29 | ||
| TW103103467 | 2014-01-29 |
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| Publication Number | Publication Date |
|---|---|
| CN104800881A CN104800881A (en) | 2015-07-29 |
| CN104800881B true CN104800881B (en) | 2017-11-03 |
Family
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| Application Number | Title | Priority Date | Filing Date |
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| CN201510046087.0A Active CN104800881B (en) | 2014-01-29 | 2015-01-29 | Biological fiber film and its preparing method |
Country Status (2)
| Country | Link |
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| CN (1) | CN104800881B (en) |
| TW (1) | TWI653060B (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2017113262A1 (en) * | 2015-12-31 | 2017-07-06 | 奈菲儿生医股份有限公司 | Antioxidative biocellulose and manufacturing method thereof |
| TWI663987B (en) * | 2016-05-17 | 2019-07-01 | 大江生醫股份有限公司 | Method for producing functional bio-cellulose membrane and the produced mask thereof |
| CN107854453A (en) * | 2017-12-18 | 2018-03-30 | 济南格莱威医疗科技有限公司 | A kind of medical aquogel eyeshield dressing |
| TWI692524B (en) * | 2018-12-28 | 2020-05-01 | 嬌朋生技股份有限公司 | Cultivated material composition |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101591626B (en) | 2009-06-30 | 2011-04-27 | 南开大学 | Strain of gluconacetobacter and application thereof |
| CN102405293A (en) * | 2010-07-09 | 2012-04-04 | 奈菲儿国际股份有限公司 | Membrane of biological fibers, and use thereof |
| CN103233050B (en) | 2013-04-22 | 2015-04-08 | 东华大学 | Bacterial cellulose membrane with gradient structure and preparation method thereof |
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2015
- 2015-01-28 TW TW104102811A patent/TWI653060B/en active
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| TW201529101A (en) | 2015-08-01 |
| TWI653060B (en) | 2019-03-11 |
| CN104800881A (en) | 2015-07-29 |
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Effective date of registration: 20230811 Address after: Chinese Taiwan New Taipei City District five shares of five power seven Road 57 six floor Patentee after: Jiaopeng Biotechnology Co.,Ltd. Patentee after: Hongqing Huajingxi Biotechnology (Guangzhou) Co.,Ltd. Address before: Chinese Taiwan New Taipei City District five shares of five power seven Road 57 six floor Patentee before: Jiaopeng Biotechnology Co.,Ltd. |