JP6463644B2 - Biological fiber membrane and method for producing the same - Google Patents
Biological fiber membrane and method for producing the same Download PDFInfo
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- 239000000835 fiber Substances 0.000 title claims description 101
- 239000012528 membrane Substances 0.000 title claims description 81
- 238000004519 manufacturing process Methods 0.000 title claims description 6
- 239000002344 surface layer Substances 0.000 claims description 29
- 239000011176 biofiber Substances 0.000 claims description 17
- 239000004480 active ingredient Substances 0.000 claims description 15
- 239000004615 ingredient Substances 0.000 claims description 13
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- 229940079593 drug Drugs 0.000 claims description 12
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- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 claims description 4
- 229930195725 Mannitol Natural products 0.000 claims description 4
- 239000008272 agar Substances 0.000 claims description 4
- 238000012258 culturing Methods 0.000 claims description 4
- 239000000594 mannitol Substances 0.000 claims description 4
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- 108090000790 Enzymes Proteins 0.000 claims description 3
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- 239000003102 growth factor Substances 0.000 claims description 3
- 239000003906 humectant Substances 0.000 claims description 3
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- SKCKOFZKJLZSFA-UHFFFAOYSA-N L-Gulomethylit Natural products CC(O)C(O)C(O)C(O)CO SKCKOFZKJLZSFA-UHFFFAOYSA-N 0.000 claims 1
- WDJHALXBUFZDSR-UHFFFAOYSA-M acetoacetate Chemical compound CC(=O)CC([O-])=O WDJHALXBUFZDSR-UHFFFAOYSA-M 0.000 claims 1
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- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 108010010803 Gelatin Proteins 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 229920000084 Gum arabic Polymers 0.000 description 1
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- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
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Description
本発明は生物繊維膜に関し、詳しくは、皮膚に貼付する生物繊維膜に関する。 The present invention relates to a biofiber membrane, and more particularly to a biofiber membrane to be applied to the skin.
現在、医療分野において、創傷治療に用いるドレッシング材の多くはカット綿またはガーゼを使用するが、それらのドレッシング材製品の抗菌性が好ましくないため、傷口の感染確率が高く、傷口にくっ付くことが起こりやすく、且つ、ドレッシング材を交換する場合は、はずし難いという欠点を有する。 Currently, in the medical field, many dressings used for wound treatment use cut cotton or gauze. However, the antibacterial properties of these dressing products are not preferred, so the probability of infection of the wound is high and it may stick to the wound. It is easy to occur and has a drawback that it is difficult to remove the dressing material.
その後、不織布ドレッシング材がガーゼ及びカット綿に取って代わったが、不織布型のドレッシング材は優れた吸液機能及び湿潤環境を提供して傷口の修復に寄与する特性を有するが、不織布が吸収する液体または水分を漸次失った後、傷口にくっ付くことが起こりやすくなる。 Later, the non-woven dressing material replaced gauze and cut cotton, but the non-woven dressing material provides excellent liquid-absorbing function and moist environment to contribute to wound repair, but the non-woven fabric absorbs After gradual loss of liquid or moisture, it tends to stick to the wound.
一方、現代人は基本的な生活での必要性のほか、さらに美容やケアを重視し、特に顔面部のケアは美容産業の訴求ポイントであるため、多くのパック製品が開発されている。現有技術のパックの種類は様々で、例えば、クレイパック、ピールオフ型パック及びシートパック等が挙げられる。 On the other hand, modern people place emphasis on beauty and care in addition to their basic needs in life. Especially, facial care is an appealing point of the beauty industry, so many pack products have been developed. There are various types of packs of the existing technology, such as clay packs, peel-off type packs, and sheet packs.
クレイパックにはケア成分またはミネラルを含有するが、パックを使用した後は洗い流す必要があり、ケア成分が完全に皮膚に吸収されるのは困難であり、且つ、多くのミネラルを含むため、より多くの防腐剤を添加して細菌が湿潤なクレイで繁殖することを防止する必要がある。ピールオフ型パックの主要成分は、ポリマーゲル、水及びアルコールであり、表皮温度を上昇させることにより、皮膚の血液循環を促すが、ピールオフ型パックはパックが乾燥してから剥がさなければならないため、剥がす過程は敏感肌に損傷を与える恐れがあり、また当該ピールオフ型パックは乾燥を求めるため、保湿成分が添加されていないので、乾燥肌には向かない。また、シートパックは主に効果を有するエッセンスを吸着した単層シートであり、成分を調整することで各種の肌質をケアすることができ、使用した後に洗い流す必要はないが、洗浄効果を有しない。上記シートパックでは、多くは単層の不織布で作ったものを使用している。使用者がエッセンスを吸着した不織布を使用する場合、通常、不織布の中の水分が速やかに揮発することを考量して、濃度が高いエッセンスを使用することから、浪費に繋がるのみならず、水分が揮発する問題も解決できない。 Clay packs contain care ingredients or minerals, but they need to be washed away after using the pack, making it difficult for the care ingredients to be completely absorbed by the skin and contain more minerals. Many preservatives need to be added to prevent bacteria from growing on wet clay. The main components of the peel-off pack are polymer gel, water and alcohol, which promotes blood circulation in the skin by raising the epidermis temperature, but the peel-off pack must be peeled off after the pack has dried The process may damage sensitive skin, and the peel-off pack is not suitable for dry skin because it does not contain moisturizing ingredients because it requires dryness. The sheet pack is a single-layer sheet that absorbs the essence that is mainly effective, and can be used to care for various skin qualities by adjusting the ingredients. do not do. Many of the above-mentioned sheet packs are made of a single layer non-woven fabric. When a user uses a nonwoven fabric that has adsorbed essence, the moisture content in the nonwoven fabric is usually taken into consideration, and the essence with a high concentration is used. The problem of volatilization cannot be solved.
そのため、新たなドレッシング材製品を開発する必要がある。 Therefore, it is necessary to develop a new dressing product.
上記公知技術の欠陥に鑑みて、本発明は、グルコンアセトバクター属の菌により形成される生物繊維膜であって、対向する第一の表層及び第二の表層と、第一の表層と第二の表層の間に結合し、密度が第一の表層及び第二の表層の密度より小さい立体網状構造と、を含む生物繊維膜を提供する。 In view of the above-mentioned deficiencies in the known technology, the present invention is a biofiber membrane formed by a bacterium of the genus Gluconacetobacter, which is a first surface layer, a second surface layer, a first surface layer, and a second surface layer that face each other. And a three-dimensional network structure having a density smaller than that of the first surface layer and the second surface layer.
一つ具体的な実施例において、立体網状構造は複数の生物繊維からなる。 In one specific embodiment, the three-dimensional network is composed of a plurality of biological fibers.
また、立体網状構造は、互いに平行する複数の骨幹繊維、及び隣接する任意の二つの骨幹繊維に織り合わされる層間繊維を有する。骨幹繊維及び層間繊維はいずれも生物繊維であり、且つ、骨幹繊維の直径は層間繊維の直径以上である。 The three-dimensional network structure has a plurality of diaphysical fibers parallel to each other and interlayer fibers interwoven with any two adjacent diaphysical fibers. Both the diaphyseal fiber and the interlayer fiber are biological fibers, and the diameter of the diaphyseal fiber is equal to or greater than the diameter of the interlayer fiber.
さらに、一つ具体的な実施例において、前記生物繊維膜は、活性成分または薬物をさらに含む。活性成分は保湿剤、美白成分、しわ防止成分、角質除去成分、成長因子（ｇｒｏｗｔｈ ｆａｃｔｏｒｓ）または酵素（ｅｎｚｙｍｅｓ）であってもよい。 Furthermore, in one specific embodiment, the biofiber membrane further comprises an active ingredient or a drug. The active ingredient may be a humectant, a whitening ingredient, an anti-wrinkle ingredient, an exfoliating ingredient, a growth factor or an enzyme.
本発明は、さらに、重量部割合で５：１：１〜４：１：１の炭素源、ペプトン及び酵母エキストラクトを有する培養液を収容する容器を提供する工程と、培養液で、グルコンアセトバクター属の菌を、２４〜９６時間培養する工程と、を含む生物繊維膜の製造方法を提供する。 The present invention further includes a step of providing a container containing a culture solution having a carbon source of 5: 1: 1 to 4: 1: 1 by weight, peptone and yeast extract, And a method for producing a biofiber membrane, comprising culturing bacteria of the genus Bacter for 24 to 96 hours.
本発明の生物繊維膜は、グルコンアセトバクター属の菌により形成される生物繊維膜であって、生物繊維膜における第一の表層と第二の表層の間に立体網状構造を有することにより、ドレッシング材の保湿性を上昇させ、より多くの活性成分を持たせることができる。 The biological fiber membrane of the present invention is a biological fiber membrane formed by a bacterium of the genus Gluconacetobacter, and has a three-dimensional network structure between the first surface layer and the second surface layer in the biological fiber membrane, thereby enabling dressing. The moisture retention of the material can be increased and more active ingredients can be provided.
以下、特定の具体的な実施例で本発明の実施形態を説明し、当業者は本明細書が開示する内容から本発明の他の利点及び効果を容易に理解できる。 The embodiments of the present invention will be described below with specific specific examples, and those skilled in the art can easily understand other advantages and effects of the present invention from the contents disclosed in the present specification.
本明細書が図面で示す構造、割合、大きさ等は、いずれも明細書が開示する内容に合わせて、当業者の理解に供するものであり、本発明の実施できる限定条件を限定するものではなく、技術上の実質的な意味を有さず、本発明が奏しうる効果及び達成できる目的に影響しない限り、任意の構造の修正、割合関係の改変、または大きさの調整は、いずれも本発明が開示する技術内容の範囲内である。同時に、本明細書が記載する「第一」、「第二」、「上」及び「一」等の用語は、単に記載の便宜上用いるものに過ぎず、本発明の実施できる範囲を限定するものではなく、その相対関係の改変または調整は、実質的に技術内容を変更しない限り、本発明の実施できる範疇内である。 The structures, ratios, sizes, and the like shown in the drawings in this specification are all for the understanding of those skilled in the art in accordance with the contents disclosed in the specification, and are not intended to limit the limiting conditions in which the present invention can be implemented. Any modification of the structure, modification of the proportion, or adjustment of the size of the present invention is not required unless it has a technical meaning and does not affect the effects that the present invention can achieve and the purpose that can be achieved. It is within the scope of the technical content disclosed by the invention. At the same time, terms such as “first”, “second”, “upper”, and “one” described in this specification are merely used for convenience of description, and limit the scope in which the present invention can be implemented. However, the modification or adjustment of the relative relationship is within the range in which the present invention can be implemented unless the technical contents are substantially changed.
本明細書における技術用語である「平行」とは、複数の骨幹繊維が同じ方向、例えば、生物繊維膜の長さ方向または幅方向で伸びる形態である。 The term “parallel” as a technical term in the present specification is a form in which a plurality of diaphyseal fibers extend in the same direction, for example, the length direction or the width direction of a biofiber membrane.
本発明は、グルコンアセトバクター属の菌により形成される生物繊維膜であって、対向する第一の表層及び第二の表層と、第一の表層と第二の表層の間に結合し、密度が第一の表層及び第二の表層の密度より小さい立体網状構造と、を含む生物繊維膜を提供する。 The present invention is a biofiber membrane formed by a bacterium of the genus Gluconacetobacter, which binds between the first surface layer and the second surface layer facing each other, and between the first surface layer and the second surface layer, and has a density. And a three-dimensional network structure smaller than the density of the first surface layer and the second surface layer.
本発明の生物繊維膜は微生物の培養から得るものである。本発明は、グルコンアセトバクター属の菌により、マンニトール、ペプトン（ｐｅｐｔｏｎｅ）、酵母エキストラクト（ｙｅａｓｔ ｅｘｔｒａｃｔ）及び寒天を有する培養液の中で形成される生物繊維膜は、複数の立体三次元に絡め合って結合する生物繊維を有することを見出した。 The biological fiber membrane of the present invention is obtained from culturing microorganisms. In the present invention, a biofiber membrane formed in a culture solution containing mannitol, peptone, yeast extract, and agar by a bacterium of the genus Gluconacetobacter is entangled in a plurality of three-dimensional dimensions. It was found to have biological fibers that bind together.
生物繊維膜を生産する具体的な実施例においては、菌種の発酵培養から始まり、まず、培養液を提供し、培養液を容器に置き、且つ、培養液の成分はゼラチン、アラビアゴム、寒天等の公知組成から選ばれるものを含み、培養液はさらに炭素源（例えばマンニトール、グルコースまたはその組合せ等）、他の成分（例えばペプトン及び酵母エキストラクト）を有し、且つ、炭素源、ペプトン及び酵母エキストラクトの重量部割合は５：１：１〜４：１：１の間であってもよい。次に、培養液を酸性、例えばｐＨ値０．５〜６の間に調整し、且つ、開始微生物濃度範囲は１０２〜１０５菌数／ｍｌに調整し、２５〜２８℃で２４〜９６時間静置培養し、又、扁平状の容器を使用できるため、前記立体網状構造は扁平膜状であり、その後、２４時間〜７２時間で取り出し、本発明の生物繊維膜が得られた。 In a specific embodiment for producing a biological fiber membrane, starting from a fermentation culture of bacterial species, first, a culture solution is provided, the culture solution is placed in a container, and the components of the culture solution are gelatin, gum arabic, agar The culture medium further has a carbon source (for example, mannitol, glucose or a combination thereof), other components (for example, peptone and yeast extract), and the carbon source, peptone and The weight fraction of yeast extract may be between 5: 1: 1 and 4: 1: 1. Next, the culture solution is adjusted to acidity, for example, between pH values 0.5 to 6, and the starting microorganism concentration range is adjusted to 10 2 to 10 5 bacteria / ml, and 24 to 96 at 25 to 28 ° C. Since the culture was allowed to stand for a period of time and a flat container could be used, the three-dimensional network structure was a flat membrane, which was then taken out in 24 to 72 hours to obtain the biological fiber membrane of the present invention.
検出したところ、生物繊維膜の厚さは、少なくとも２０μｍ、例えば２０〜３０μｍ、または、例えば２０〜２６μｍまたは２４〜２６μｍであり、本発明の生物繊維膜の生物繊維の直径は、約２０〜１００ｎｍである。又、生物繊維膜の単位面積ごとの生物繊維量は０．００５〜０．００８ｇ／ｃｍ２である。 As detected, the thickness of the biofiber membrane is at least 20 μm, such as 20-30 μm, or such as 20-26 μm or 24-26 μm, and the biofiber diameter of the biofiber membrane of the present invention is about 20-100 nm. It is. Moreover, the amount of biological fibers per unit area of the biological fiber membrane is 0.005 to 0.008 g / cm 2 .
図１が示すように、本発明の生物繊維膜１は、対向する第一の表層１０ａ及び第二の表層１０ｂと、第一の表層１０ａと第二の表層１０ｂの間に結合する立体網状構造１０１を含む。 As shown in FIG. 1, the biological fiber membrane 1 of the present invention includes a first surface layer 10a and a second surface layer 10b facing each other, and a three-dimensional network structure bonded between the first surface layer 10a and the second surface layer 10b. 101.
なお、図２Ａに示すように、第二の表層１０ｂから立体網状構造１０１が伸びており、布膜である繊維１２の上で結合している。 In addition, as shown to FIG. 2A, the solid network structure 101 is extended from the 2nd surface layer 10b, and it has couple | bonded on the fiber 12 which is a cloth film.
図に示すように、立体網状構造１０１は、第二の表層１０ｂから布膜の繊維１２まで伸びて、且つ、立体網状構造１０１は複数の生物繊維からなる。具体的には、立体網状構造１０１は互いに平行する複数の骨幹繊維１０１ａ及び隣接する任意の二つの骨幹繊維１０１ａに織り合わされる層間繊維１０１ｂを有することで、水平方向及び垂直方向で隣接する任意の二つの骨幹繊維１０１ａを連接し、三次元立体網状構造１０１を形成する。骨幹繊維１０１ａ及び層間繊維１０１ｂはいずれも生物繊維であり、且つ、図に示すように、骨幹繊維１０１ａの直径は層間繊維１０１ｂの直径以上である。 As shown in the figure, the three-dimensional network structure 101 extends from the second surface layer 10b to the fabric fibers 12, and the three-dimensional network structure 101 is composed of a plurality of biological fibers. Specifically, the three-dimensional network structure 101 includes a plurality of diaphyseal fibers 101a parallel to each other and an interlayer fiber 101b interwoven with any two adjacent diaphyseal fibers 101a, thereby allowing any arbitrary adjoining in the horizontal direction and the vertical direction. Two skeleton fibers 101a are connected to form a three-dimensional three-dimensional network structure 101. The diaphyseal fiber 101a and the interlayer fiber 101b are both biological fibers, and as shown in the figure, the diameter of the diaphyseal fiber 101a is equal to or greater than the diameter of the interlayer fiber 101b.
図２Ｂは生物繊維膜１の側面写真を示し、生物繊維膜１は、互いに平行するまたは生物繊維膜１の長さ方向もしくは幅方向に伸びる複数の骨幹繊維１０１ａと、骨幹繊維１０１ａに織り合わされる層間繊維１０１ｂとを有し、この実施例において、生物繊維膜１の厚さは２０〜３０μｍである。また、図に示すように、立体網状構造の密度は生物繊維膜１の両表層の密度より小さく、活性成分または薬物は両表層の間に付着し、保湿効果を提供できる。 FIG. 2B shows a side view photograph of the biological fiber membrane 1. The biological fiber membrane 1 is interwoven with the bone shaft fibers 101a and a plurality of bone shaft fibers 101a extending in the longitudinal direction or the width direction of the biological fiber membrane 1. In this embodiment, the thickness of the biological fiber membrane 1 is 20 to 30 μm. Moreover, as shown in the figure, the density of the three-dimensional network structure is smaller than the density of both surface layers of the biological fiber membrane 1, and the active ingredient or the drug adheres between both surface layers and can provide a moisturizing effect.
また、生物繊維膜の製造では、基材を使用し、膜体を一時的にまたは恒久的に結合できるが、いずれにせよ、この製造方法は、上述した容器内で扁平状立体網状構造をひっくり返す工程を省略できる。例えば、図２Ａの実施例においては、布膜を基材として使用する。 Moreover, in the production of a biofiber membrane, a substrate can be used to bond the membrane body temporarily or permanently, but in any case, this production method flips the flat three-dimensional network structure in the container described above. The process can be omitted. For example, in the embodiment of FIG. 2A, a fabric film is used as the substrate.
図３Ａ及び図３Ｂは、それぞれ、本発明の生物繊維膜の５００倍で拡大したＳＥＭ写真、及び従来生物繊維膜の５００倍で拡大したＳＥＭ写真を示す。 FIG. 3A and FIG. 3B show an SEM photograph magnified by 500 times of the biological fiber membrane of the present invention and an SEM photograph magnified by 500 times of the conventional biological fiber membrane, respectively.
図３Ａに示すように、本発明の生物繊維膜の表面は平坦であり、図面で示す条状物は膜体下方の布膜基材の繊維であり、本発明の生物繊維膜の表面は非常に平坦である。しかし、図３Ｂに示す従来生物繊維膜の表面は多くのしわを有する。そのため、生物繊維膜と皮膚の付着性がよくないため、感触に影響し、薬物または活性成分の吸収がよくないという問題を起こす。 As shown in FIG. 3A, the surface of the biological fiber membrane of the present invention is flat, and the strips shown in the drawing are fibers of the cloth membrane base material below the membrane body, and the surface of the biological fiber membrane of the present invention is very It is flat. However, the surface of the conventional biological fiber membrane shown in FIG. 3B has many wrinkles. Therefore, the adhesion between the biofiber membrane and the skin is not good, which affects the touch and causes the problem of poor absorption of drugs or active ingredients.
本発明の生物繊維膜の単位面積ごとの生物繊維量及び含水量試験
本発明の生物繊維膜を、５ｃｍｘ５ｃｍのサイズに切断し、全部で１６片のサンプルを作った後、６０℃で１０分間ベーク乾燥し、ベーク乾燥したサンプルの乾燥重量を測る。サンプルの面積を各サンプルの乾燥重量で割って、求められた生物繊維膜の単位面積ごとの生物繊維量は０．００５〜０．００８ｇ／ｃｍ２である。
Biological fiber content and water content test for each unit area of the biological fiber membrane of the present invention The biological fiber membrane of the present invention was cut into a size of 5 cm x 5 cm, and a total of 16 pieces of samples were made, followed by baking at 60 ° C for 10 minutes. Weigh the dry weight of the dried and baked sample. Dividing the area of the sample by the dry weight of each sample, the amount of biological fiber per unit area of the biological fiber membrane determined is 0.005 to 0.008 g / cm 2 .
さらに、それらのサンプルを水に５分間浸して、それらのサンプルの湿重量を測り、単位面積ごとの含水量を測る際は、下記式で算出する。
単位面積ごとの含水量＝（湿重量−乾燥重量）／サンプル面積
Furthermore, when these samples are immersed in water for 5 minutes, the wet weight of these samples is measured, and the water content for each unit area is measured, the following formula is used.
Water content per unit area = (wet weight-dry weight) / sample area
検出したところ、本発明の生物繊維膜の単位面積ごとの含水量は０．０６〜０．１４ｇ／ｃｍ２であった。 As a result of detection, the water content per unit area of the biological fiber membrane of the present invention was 0.06 to 0.14 g / cm 2 .
それに対して、市販の従来生物繊維膜の単位面積ごとの生物繊維量は、通常、０．００１ｇ／ｃｍ２にとどまり、含水量は０．０４ｇ／ｃｍ２にとどまる。したがって、本発明の生物繊維膜はより多くの水分または活性成分を有し、更なる保湿効果を有する。 On the other hand, the amount of biological fibers per unit area of a commercially available conventional biological fiber membrane is usually only 0.001 g / cm 2 , and the water content is only 0.04 g / cm 2 . Therefore, the biofiber membrane of the present invention has more moisture or active ingredients and has a further moisturizing effect.
本発明の生物繊維膜の浸透性試験
本発明の生物繊維膜及び市販の従来生物繊維膜を、それぞれ底材の上に設置し、次に、本発明の生物繊維膜及び市販の従来生物繊維膜に、等量の染色されたエッセンスを滴下し、１０秒後、底材の浸透状況を目視で観察した。
The permeability test of the biological fiber membrane of the present invention The biological fiber membrane of the present invention and the commercially available conventional biological fiber membrane are respectively installed on the bottom material, and then the biological fiber membrane of the present invention and the commercially available conventional biological fiber membrane Then, an equal amount of the dyed essence was dropped, and after 10 seconds, the state of penetration of the bottom material was visually observed.
図４に示すように、図４（ａ）は本発明の生物繊維膜の試験結果であり、図４（ｂ）は市販の従来生物繊維膜の試験結果である。その中、市販の従来生物繊維膜は、エッセンスを滴下した時、そのエッセンスの拡散効果は良くなく、１０秒後にはエッセンスを底材まで自然に効果的に浸透させることができなかった。それに対して、本発明の生物繊維膜は優れたエッセンス拡散性及び顕著的な浸透性を有する。 As shown in FIG. 4, FIG. 4 (a) is a test result of the biological fiber membrane of the present invention, and FIG. 4 (b) is a test result of a commercially available conventional biological fiber membrane. Among them, commercially available conventional biological fiber membranes did not have a good effect of diffusing the essence when the essence was dropped, and after 10 seconds, the essence could not be naturally and effectively penetrated into the bottom material. In contrast, the biological fiber membrane of the present invention has excellent essence diffusibility and significant permeability.
以上のように、本発明の生物繊維膜は皮膚に貼付し、特にパックとして使用できるため、生物繊維膜はさらに活性成分または薬物を含んでもよい。菌種の発酵培養終了前に、活性成分または薬物を立体網状構造上に投下し、生物繊維膜の製作が完成したら、活性成分または薬物は膜体内に含まれる。活性成分の実例には、保湿剤、美白成分、しわ防止成分、角質除去成分、成長因子（ｇｒｏｗｔｈ ｆａｃｔｏｒｓ）または酵素（ｅｎｚｙｍｅｓ）が含まれる。薬物はマッサージ薬物であってもよく、クリーム状または液体であってもよく、例えばマッサージまたはストレス解消用精油等であってもよい。本発明の生物繊維膜が立体網状構造を有するため、活性成分または薬物は簡単に露出することはなく、本発明の生物繊維膜が体表または皮膚上に付着した後、人力マッサージまたは他の接触装置により生物繊維膜に加圧することで、活性成分または薬物の放出に寄与でき、さらに、加温することにより活性成分または薬物の放出に寄与できる。 As described above, since the biological fiber membrane of the present invention is affixed to the skin and can be used particularly as a pack, the biological fiber membrane may further contain an active ingredient or a drug. When the active ingredient or drug is dropped on the three-dimensional network structure before the fermentation culture of the bacterial species is completed and the production of the biofiber membrane is completed, the active ingredient or drug is contained in the membrane. Examples of active ingredients include humectants, whitening ingredients, wrinkle prevention ingredients, exfoliating ingredients, growth factors or enzymes. The drug may be a massage drug, may be creamy or liquid, and may be, for example, a massage or a stress relieving essential oil. Since the biofibrous membrane of the present invention has a three-dimensional network structure, the active ingredient or drug is not easily exposed, and after the biofibrous membrane of the present invention is deposited on the body surface or the skin, human massage or other contact is performed. By pressurizing the biological fiber membrane with the device, it can contribute to the release of the active ingredient or drug, and further, it can contribute to the release of the active ingredient or drug by heating.
上記実施例は例示的に本発明の原理及びその効果を説明するためのものであり、本発明を制限するものではない。当業者は本発明の主旨及び範疇に違反しない限り、上記実施例を改変できる。本発明の権利保護範囲は、特許請求の範囲の通りである。 The above-described embodiments are for illustrative purposes only and are not intended to limit the present invention. Those skilled in the art can modify the above-described embodiments without violating the spirit and scope of the present invention. The scope of rights protection of the present invention is as set forth in the claims.
１ 生物繊維膜
１０ａ 第一の表層
１０ｂ 第二の表層
１０１ 立体網状構造
１０１ａ 骨幹繊維
１０１ｂ 層間繊維
１２ 繊維
DESCRIPTION OF SYMBOLS 1 Biological fiber membrane 10a First surface layer 10b Second surface layer 101 Three-dimensional network structure 101a Skeletal fiber 101b Interlayer fiber 12 Fiber
Claims (6)
対向する第一の表層及び第二の表層と、
前記第一の表層と前記第二の表層の間に結合し、密度が前記第一の表層及び前記第二の表層の密度より小さい立体網状構造と、
を含み、
前記立体網状構造は、骨幹繊維及び層間繊維を有し、
前記骨幹繊維は、複数で存在して、互いに平行し、
前記層間繊維は、複数で存在して、隣接する任意の二つの前記骨幹繊維に織り合わされ、
前記骨幹繊維の直径は前記層間繊維の直径以上であり、
前記生物繊維膜の厚さは、２４〜２６μｍであり、
前記生物繊維膜の生物繊維の直径は、２０〜１００ｎｍであり、
前記生物繊維膜の単位面積ごとの含水量は、０．０６〜０．１４ｇ／ｃｍ２であり、
前記生物繊維膜の単位面積ごとの生物繊維量は０．００５〜０．００８ｇ／ｃｍ２である生物繊維膜。 Glucon acetoacetate in a culture solution having 5: 1: 1 to 4: 1: 1 mannitol, peptone, yeast extract in a weight part ratio, containing agar and having a pH value of 0.5-6. A biofibrous membrane formed by culturing bacteria of the genus Bacter for 24 to 96 hours ,
Opposing first and second surface layers;
A three-dimensional network structure bonded between the first surface layer and the second surface layer, the density being smaller than the density of the first surface layer and the second surface layer;
Including
The three-dimensional network structure has skeletal fibers and interlayer fibers,
The diaphyseal fiber is present in a plurality, parallel to each other,
A plurality of the interlayer fibers are interwoven with any two adjacent diaphyseal fibers,
The diameter of the diaphyseal fiber is not less than the diameter of the interlayer fiber,
The biological fiber membrane has a thickness of 24 to 26 μm,
The biological fiber diameter of the biological fiber membrane is 20-100 nm,
The water content per unit area of the biological fiber membrane is 0.06 to 0.14 g / cm 2 ,
The biological fiber membrane in which the biological fiber amount per unit area of the biological fiber membrane is 0.005 to 0.008 g / cm 2 .
前記培養液で、グルコンアセトバクター属の菌を、２４〜９６時間培養する工程と、
を含む、
請求項１〜５のいずれかに記載の生物繊維膜の製造方法。 Provided is a container for containing a culture solution containing mannitol , peptone and yeast extract in a weight ratio of 5: 1: 1 to 4: 1: 1, containing agar and having a pH value of 0.5-6. And a process of
Culturing bacterium of the genus Gluconacetobacter in the culture solution for 24 to 96 hours;
including,
The manufacturing method of the biological fiber membrane in any one of Claims 1-5 .
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