CN104799051A - Preparation method of ecological razor clam feed - Google Patents
Preparation method of ecological razor clam feed Download PDFInfo
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- CN104799051A CN104799051A CN201510127970.2A CN201510127970A CN104799051A CN 104799051 A CN104799051 A CN 104799051A CN 201510127970 A CN201510127970 A CN 201510127970A CN 104799051 A CN104799051 A CN 104799051A
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Abstract
The invention discloses a preparation method of ecological razor clam feed and belongs to the field of aquaculture. The quality and the quantity of the feed are bottleneck factors restricting high yield and stable yield of razor clams in the culture process of the razor clams. According to a traditional razor clam feed preparation method, equipment is complicated, feed is prone to rot and go bad, ingredients are monotonous, nutrition is incomplete, the preparation process is complicated, and the cost is higher. According to the razor clam feed prepared with the method, the ecological razor clam feed is prepared by mixing five kinds of marine microalgae serving as main materials and auxiliary materials comprising the fish meal, blood meal, cow manure, fowl manure, chlorella powder, beer yeast, silkworm chrysalis meal, peanut cakes, tenebrio molitor powder and earthworm powder, has complete nutrition, facilitates quick growth and development of the razor clams, is seldom lost after scattered in water and can be conveniently scattered into water for feeding, raw materials are easy to obtain, the preparation method and equipment are simple, and the cost is low.
Description
Technical field the invention belongs to aquaculture field, particularly relates to a kind of preparation method of razor clam ecological bait.
Background technology
Razor clam has another name called razor clam king, is subordinate to Mollusca, Bivalvia, Eulamellibranchia, razor clam section, and razor clam belongs to, and is marine products bivalve mollusks, and body is in extending shape, and two shells are thick bamboo tube shape after filling the span of a man's arms, therefore razor clam of gaining the name.Body surface smooth, drapes over one's shoulders yellowish-brown crust, and glossy, chitin is thin and brittle, and old individual former crust often comes off and in white; Face is given prominence to, and back of the body edge is parallel with ventral margin, and the pure circle in rear end, is rectangle, and growth line is obvious, is about 11 centimetres, as the bamboo chip that two pieces break.Shell inner face white, what have can see pale red colored ribbon.Muscle of foot yellow-white, very flourishing; Anterior adductor muscle trace is long, posterior adductor muscle trace triangle.Outlet pipe and oral siphon often stretch out outside shell, short and thick.Perch in shallow sea and intertidal zone sand, perch of burrowing, as put a little salt solution in cave, razor clam can jump out by cave, and time frightened, water pipe is easily autotomyed.Razor clam happiness inhabits the husky mud bank in Zhong Xia tidal zone, intertidal zone to shallow sea, the silt substrate of outstanding happiness silt content 70-80%, uprightly lives with powerful anchor shape abdominal foot, health major part is imbedded sand mud, if meet dangerous or environment is bad, autotomy its go out, oral siphon and rapidly health is all imbedded sand mud.Razor clam is a kind of marine products high-quality shellfish, and perch in the lower and depth of water 20 to the five ten centimetres of marine sites of salinity, China is coastal from north to south all has distribution.Kind is more, and continent southeastern coast output is higher, and then fecund is in the middle and south for TaiWan, China, and especially in platform and Yun Jiayi band silt marine site, kind mainly contains razor shell, Solen strictus, Solen gracilis and short razor clam etc.Suitable temperature range 2-32 DEG C, the suitableeest 15-27 DEG C, mainly ingest phytoplankton and organic debris.Razor clam gonochorism, 5-7 month breeding period, embryonated egg marine spend one section of life of swimming after, develop into veliger successively, after progressively transfer the end to and dwell.Razor clam, also can artificial fecundation and cultivation except self-sow, and razor shell and Solen strictus are because individuality is large, growth is fast and become main breed variety.Cultivate 2 years razor clam body length and can reach 8 centimetres, large person 22 centimetres.Razor clam muscle of foot is flourishing, and be rich in the nutrition such as protein, fat, carbohydrate, ash content and calcium, phosphorus, iron, iodine and vitamin, be a kind of delicious flavour, nutritious precious marine product, breeding prospect is wide.Razor clam also has medical value, and according to medicine, secretary is carried, razor clam shell have dissipating bind, anti-inflammatory, treating stranguria, be only with effect, razor clam meat then have bring down a fever, improving eyesight, quench the thirst, relieving alcoholism and cure women and to strain the function such as lower blood and logical breast.
In razor clam breeding process, food quality and quantity are restriction razor clam high yield and stable yields bottleneck factor.Past, Wang Baoshan discloses one and to hang razor clam biological feed and preparation method (application number CN200910262879), described bait is made up of following weight proportion raw material: fish meal 20-40, blood meal 3-7, dregs of beans 2-4, Testa Glycines 3-7, bean dregs 8-12, dish dregs of rice 10-20, cotton dregs 3-17, maize gluten feed 10-15, rice bran 2-4, wheat bran 1-3, betaine 0.02-0.05, calcium monohydrogen phosphate 0.08-0.15, mixed bacteria powder 3-8, and this invention also relates to this razor clam biological feed preparation method that hangs.This bait contains a large amount of mycoprotein, outer mould, the amylomyces isoreactivity bioprotein of born of the same parents, and bait protein content reaches 38-40%, and finished product is rich in animal protein, vegetable protein and mycoprotein and has special yeast fragrance.This food nutrition is comprehensive, and in albumen, amino acid composition is in a basic balance, and adapt to razor clam breeding environment and biological nature, in water, suspension is good, can directly be assimilated by razor clam, but making trouble, equipment is complicated, and cost is higher, easily rots, goes bad.In addition, Wang Xing has invented by force one and to have hung constricta zoology mixed feed (application number CN200710021209), described feed is made up of less than 16 kinds percentage by weight natural materials: white fish meal 15-25%, Soybean Meal 5-15%, peanut meal 3-8%, scallop edge 2-10%, shrimp head powder 2-10%, brewer's yeast 2-8%, Kelp Powder 10-25%, laver 8-15%, sea lettuce 1-8%, spirulina powder 1-2%, Dunaliella salina 2-5%, ocean fish oil 1-2%, soybean lecithin 0.5-1%, attapulgite clay 3-12%, eupatorium lindleynun var. trifoliolatum 0.1-0.2%, mashed garlic 0.1-0.2%.Product does not contain chemical addition agent, feed good palatability, and conversion ratio is high, little to water pollution; Feed and hang razor clam growth soon, survival rate is high; Energy adsorbed water body and harmful substance in razor clam body of hanging; This feedstuff is easy to get, and production technology is simple, and steady quality, efficiently solves razor clam of hanging and propagate feed problem artificially and be conducive to environmental protection, but this bait composition is dull, and not comprehensive nutrition, complex manufacturing technology, cost is higher.
In order to overcome the deficiency of current technology, the invention discloses a kind of preparation method of razor clam ecological bait.Compared with conventional art, novelty of the present invention shows: 1) razor clam bait is by 5 kinds of micro-algaes and fish meal, blood meal, cow dung, chicken manure, chlorella powder, beer yeast powder, dried silkworm chrysalis meal, peanut cake, yellow meal worm powder, earthworm powder mixed preparing forms, nutrition more comprehensively, science, rationally, be conducive to the quick g and D of razor clam; 2) utilize magnetized drinking water and green light also to use the micro-algae of medium culture optimized, micro algae growth is fast, and output is high, and in micro-algae, the contour unsaturated fatty acid content of EPA and DHA is high, is conducive to razor clam and grows and breed; 3) break traditions micro-algae monoculture pattern, 5 kinds of micro-algaes are adopted to raise together with, improve illumination and space availability ratio, 5 kinds of micro-algaes are mutually promoted, in microdisk electrode process, respect micro algae growth rule, the early, middle and late phase of cultivating provides different temperatures and intensity of illumination and light application time, early stage temperature is lower, illumination is more weak, and the light injury avoiding high light to cause frustule, is conducive to micro algae growth, higher temperature and comparatively intense light irradiation are provided late period, are conducive to the nutritious substances accumulation such as EPA and DHA in cell; 4) secondary cultivation is adopted to cultivate micro-algae, be conducive to the raising of micro-algae output, particularly the second level adopts two bottle,suction series connection air-charging incubation, the gas be filled with obtains secondary and utilizes, and saved energy and resource, and equipment is simple, not easily pollute, scrub conveniently, growth is fast, micro-algae output increased 140%; 5) micro-algae is collected and adopts centrifugal process traditionally, and centrifuge is expensive, and power consumption is many, expends huge, and this technology adopts compound precipitants, and fast, thoroughly, equipment is simple, and cost is low, easy and simple to handle for precipitation.
The biological property of table 15 kinds of bait micro-algaes
Summary of the invention
In order to overcome the deficiency of current technology, the invention discloses a kind of preparation method of razor clam ecological bait, the algae cream that this bait is made with 5 kinds of marine microalgaes is for main component, be aided with fish meal, blood meal, cow dung, chicken manure, chlorella powder, beer yeast powder, dried silkworm chrysalis meal, peanut cake, yellow meal worm powder, earthworm powder by a certain percentage mixing manufacture forms, and concrete grammar is as follows:
The preparation IBDU of step 1 micro algae culturing liquid 0.3 part, oxamides 0.1 part, ammonium magnesium phosphate 0.02 part, APP 0.01 part, 0.05 part, calcium polyphosphate potassium, 0.02 part, ammonium sulfate, 0.04 part, ammonium nitrate, 0.1 part, urea, potassium sulfate 0.02 part, 0.6 part, potassium chloride, ammonium carbonate 8 parts, gibberellin 0.005 part, spermidine 0.008 part, f/2 trace element solution 1.2 parts, f/2 vitamin solution 1.2 parts, scalding and magnetization after 1000 parts, clean seawater.Acetic acid solution (volume ratio) with 15% and NaOH solution (weight ratio) adjust ph of 10% are to 7.8-8.2.Wherein f/2 trace element solution formula: ZnSO
44H
2o 23 milligrams, MnCl
24H
2o 178 milligrams, CuSO
45H
2o 10 milligrams, FeC
6h
5o
75H
2o 3.9 grams, Na
2moO
42H
2o 7.3 milligrams, CoCl
26H
2o 12 milligrams, Na
2eDTA 4.35 grams, distilled water 1000 milliliters; F/2 vitamin solution is filled a prescription: Cobastab
120.5 milligram, Cobastab
1100 milligrams, biotin 0.5 milligram, distilled water 1000 milliliters.
The one-level of the micro-algae of step 2 is cultivated and is got 1000 milliliters of circular flat bottom flasks, wash, add 200 ml distilled waters, electric furnace boils 3 minutes, sterilization, after outwell distilled water, cooling, add nutrient solution described in step 1 500 milliliters, inoculate respectively with rainbow rotary strainer algae that is pollution-free, pure, that be in exponential phase of growth, spot hailian seaweed, Skeletonema Costatum, tip boat-shaped algae, Phaeodactylum tricornutum, 5 kinds of micro-algae Mixed culture.Inoculum density is rainbow rotary strainer algae 2.5 ten thousand/milliliter respectively, spot hailian seaweed 30,000/milliliter, Skeletonema Costatum 1.5 ten thousand/milliliter, tip boat-shaped algae 40,000/milliliter, Phaeodactylum tricornutum 1.8 ten thousand/milliliter; Shake up gently, cover bottleneck with the clean newspaper of individual layer, rubber band ties, and puts in illumination box and cultivates.Do not inflate, every day manual shaking flask 6 times, each 3 minutes.First 3 days that cultivate, temperature was set to 18 DEG C, intensity of illumination 2500lux, light dark period L: D=12h: 12h, i.e. 12 h light, and 12 h dark, so circulate; The 4-6 days cultivated, temperature is adjusted to 24 DEG C, and intensity of illumination is set to 6000lux, and light dark period is set to L: D=14h: 10h, i.e. 14 h light, then 10 h dark, so moved in circles, by the 6th day, the growth of micro-algae reaches the peak of exponential phase of growth, density sum can reach 1,200 ten thousand/milliliters, stops cultivating, and proceeds to next stage and cultivates.
The secondary of the micro-algae of step 3 cultivates the bottle,suction getting 2 3000 milliliters, and (chemical apparatuses company is on sale, this bottle is similar to common conical flask, just with upper and lower two smallmouths), after washing, use distilled water rinse again 3 times, the high mouth of first bottle is connected with emulsion tube or plastic tube with the low mouth of second bottle, junction does not leak gas, each bottle adds the micro algae culturing liquid 1500 milliliters described in step 1, then the algae liquid after cultivating by step 2 does seed liquor inoculation, inoculum concentration is 6: 1 (volume ratios), and namely 6 parts of nutrient solutions add 1 part of seed liquor; Then the low mouth of first bottle is connected with air compressor, and bottleneck rubber stopper clogs, and like this, the gas be filled with can obtain secondary and utilize; Be filled with mist, gas componant and volume ratio are V
air: V
purity oxygen: V
pure carbon dioxide: V
pure chlorine=97: 1.2: 1.3: 0.5, aeration quantity should not be too large, frustule can be allowed to suspend and do not precipitate.Between-line spacing is inflated, and namely inflates 1 hour, stops 1 hour, repeat.With green plastic film, bottle,suction is covered, irradiate the mainly green spectral of algae liquid like this.Be placed on microdisk electrode room by these 2 bottles to cultivate, culturing room is by air-conditioning temperature control, and fluorescent tube provides illumination, first 4 days that cultivate, set temperature 20 DEG C, intensity of illumination 3600lux, light dark period L: D=12h: 12h, 5-7 days, adjusts the temperature to 23 DEG C, intensity of illumination 4500lux, light dark period L: D=16h: 8h, i.e. illumination 16 hours, dark 8 hours, repeats; 8-10 days, adjust the temperature to 25 DEG C, intensity of illumination 9000lux, whole day is set to illumination, does not have dark.By the 8th day, the nutrient solution 100 milliliters described in step 1 added by each bottle, with the consumption of the salt that supplements the nutrients, and adds 50 ml distilled waters, and by the 10th day, micro-algae density sum can reach 1,800 ten thousand/milliliters, stopped cultivating, and carried out frustule collection.
Collection preparing quick lime, copper sulphate, the chitin of the micro-algae of step 4, in quick lime: copper sulphate: chitin=1: the ratio mixing of 0.8: 2 (weight ratio), with mixer mix and blend 20 minutes, to even, make compound precipitants, get algae liquid described in step 3, the ratio adding 120 milligrams of compound precipitantses in 1 liter of algae liquid adds precipitating reagent, and glass bar stirs, and leaves standstill, precipitates 24 hours, frustule sinks to bottom and forms toothpaste-like algae cream, abandon supernatant, obtain algae cream, load in the polybag of dress food, insert-4 DEG C of Refrigerator stores, for subsequent use.
The preparation bait formula of step 5 razor clam ecological bait: algae cream 28 parts, fish meal 0.6 part, blood meal 1.2 parts, cow dung 0.8 part, chicken manure 1.5 parts, chlorella powder 0.3 part, beer yeast powder 0.2 part, dried silkworm chrysalis meal 1 part, 0.1 part, peanut cake, 0.2 part, yellow meal worm powder, earthworm powder 0.7 part.Wherein powder particles size is 25-80 micron, and mixing and blending machine stirs, and bait completes, razor clam of can throwing something and feeding.
Beneficial outcomes
In order to overcome the deficiency of current technology, the invention discloses a kind of preparation method of razor clam ecological bait.Compared with conventional art, novelty of the present invention shows: 1) razor clam bait is by 5 kinds of micro-algaes and fish meal, blood meal, cow dung, chicken manure, chlorella powder, beer yeast powder, dried silkworm chrysalis meal, peanut cake, yellow meal worm powder, earthworm powder mixed preparing forms, nutrition more comprehensively, science, rationally, be conducive to the quick g and D of razor clam; 2) utilize magnetized drinking water and green light also to use the micro-algae of medium culture optimized, micro algae growth is fast, and output is high, and in micro-algae, the contour unsaturated fatty acid content of EPA and DHA is high, is conducive to razor clam and grows and breed; 3) break traditions micro-algae monoculture pattern, 5 kinds of micro-algaes are adopted to raise together with, improve illumination and space availability ratio, 5 kinds of micro-algaes are mutually promoted, in microdisk electrode process, respect micro algae growth rule, the early, middle and late phase of cultivating provides different temperatures and intensity of illumination and light application time, early stage temperature is lower, illumination is more weak, and the light injury avoiding high light to cause frustule, is conducive to micro algae growth, higher temperature and comparatively intense light irradiation are provided late period, are conducive to the nutritious substances accumulation such as EPA and DHA in cell; 4) secondary cultivation is adopted to cultivate micro-algae, be conducive to the raising of micro-algae output, particularly the second level adopts two bottle,suction series connection air-charging incubation, the gas be filled with obtains secondary and utilizes, and saved energy and resource, and equipment is simple, not easily pollute, scrub conveniently, growth is fast, micro-algae output increased 140%; 5) micro-algae is collected and adopts centrifugal process traditionally, and centrifuge is expensive, and power consumption is many, expends huge, and this technology adopts compound precipitants, and fast, thoroughly, equipment is simple, and cost is low, easy and simple to handle for precipitation.
Accompanying drawing explanation
Fig. 1 is rainbow rotary strainer algae form;
Fig. 2 is spot hailian seaweed form;
Fig. 3 is Skeletonema Costatum form;
Fig. 4 is tip boat-shaped algae form;
Fig. 5 is Phaeodactylum tricornutum form;
Fig. 6 is micro-algae one-level cultural method;
Fig. 7 is micro-algae secondary cultural method, and arrow is depicted as gas trend.
Detailed description of the invention
A preparation method for razor clam ecological bait, the algae cream that this bait is made with five kinds of marine microalgaes, for main component, is aided with fish meal, blood meal, cow dung, chicken manure, chlorella powder, beer yeast powder, dried silkworm chrysalis meal, peanut cake, yellow meal worm powder, earthworm powder in proportion mixing manufacture forms, and concrete steps are as follows:
The preparation of step 1 micro algae culturing liquid
By weight: IBDU 0.3 part, oxamides 0.1 part, ammonium magnesium phosphate 0.02 part, APP 0.01 part, 0.05 part, calcium polyphosphate potassium, 0.02 part, ammonium sulfate, 0.04 part, ammonium nitrate, 0.1 part, urea, potassium sulfate 0.02 part, 0.6 part, potassium chloride, ammonium carbonate 8 parts, gibberellin 0.005 part, spermidine 0.008 part, f/2 trace element solution 1.2 parts, f/2 vitamin solution 1.2 parts, scalding and magnetization after 1000 parts, clean seawater; Acetic acid solution (volume ratio) with 15% and NaOH solution (weight ratio) adjust ph of 10% are to 7.8-8.2; Wherein f/2 trace element solution formula: ZnSO
44H
2o 23 milligrams, MnCl
24H
2o 178 milligrams, CuSO
45H
2o 10 milligrams, FeC
6h
5o
75H
2o 3.9 grams, Na
2moO
42H
2o 7.3 milligrams, CoCl
26H
2o 12 milligrams, Na
2eDTA 4.35 grams, distilled water 1000 milliliters; F/2 vitamin solution is filled a prescription: Cobastab
120.5 milligram, Cobastab
1100 milligrams, biotin 0.5 milligram, distilled water 1000 milliliters;
The one-level of the micro-algae of step 2 is cultivated
Get 1000 milliliters of circular flat bottom flasks, wash, add 200 ml distilled waters, electric furnace boils 3 minutes, sterilization, after outwell distilled water, cooling, add nutrient solution described in step 1 500 milliliters, inoculate respectively with rainbow rotary strainer algae that is pollution-free, pure, that be in exponential phase of growth, spot hailian seaweed, Skeletonema Costatum, tip boat-shaped algae, Phaeodactylum tricornutum, 5 kinds of micro-algae Mixed culture; Inoculum density is rainbow rotary strainer algae 2.5 ten thousand/milliliter respectively, spot hailian seaweed 30,000/milliliter, Skeletonema Costatum 1.5 ten thousand/milliliter, tip boat-shaped algae 40,000/milliliter, Phaeodactylum tricornutum 1.8 ten thousand/milliliter; Shake up gently, cover bottleneck with the clean newspaper of individual layer, rubber band ties, and puts in illumination box and cultivates; Do not inflate, every day manual shaking flask 6 times, each 3 minutes.First 3 days that cultivate, temperature was set to 18 DEG C, intensity of illumination 2500lux, light dark period L: D=12h: 12h, i.e. 12 h light, and 12 h dark, so circulate; The 4-6 days cultivated, temperature is adjusted to 24 DEG C, and intensity of illumination is set to 6000lux, and light dark period is set to L: D=14h: 10h, i.e. 14 h light, then 10 h dark, so moved in circles, by the 6th day, the growth of micro-algae reaches the peak of exponential phase of growth, density sum can reach 1,200 ten thousand/milliliters, stops cultivating, and proceeds to next stage and cultivates;
The secondary of the micro-algae of step 3 is cultivated
Get the bottle,suction of 2 3000 milliliters, after washing, use distilled water rinse again 3 times, the high mouth of first bottle is connected with emulsion tube or plastic tube with the low mouth of second bottle, junction does not leak gas, and each bottle adds the micro algae culturing liquid 1500 milliliters described in step 1, and the algae liquid after then cultivating by step 2 does seed liquor inoculation, inoculum concentration is 6: 1 (volume ratios), and namely 6 parts of nutrient solutions add 1 part of seed liquor; Then the low mouth of first bottle is connected with air compressor, and bottleneck rubber stopper clogs, and like this, the gas be filled with can obtain secondary and utilize; Be filled with mist, gas componant and volume ratio are V
air: V
purity oxygen: V
pure carbon dioxide: V
pure ammonia=97: 1.2: 1.3: 0.5, aeration quantity should not be too large, frustule can be allowed to suspend and do not precipitate; Between-line spacing is inflated, and namely inflates 1 hour, stops 1 hour, repeat; With green plastic film, bottle,suction is covered, irradiate the mainly green spectral of algae liquid like this; Be placed on microdisk electrode room by these 2 bottles to cultivate, culturing room is by air-conditioning temperature control, and fluorescent tube provides illumination, first 4 days that cultivate, set temperature 20 DEG C, intensity of illumination 3600lux, light dark period L: D=12h: 12h, 5-7 days, adjusts the temperature to 23 DEG C, intensity of illumination 4500lux, light dark period L: D=16h: 8h, i.e. illumination 16 hours, dark 8 hours, repeats; 8-10 days, adjust the temperature to 25 DEG C, intensity of illumination 9000lux, whole day is set to illumination, does not have dark; By the 8th day, the nutrient solution 100 milliliters described in step 1 added by each bottle, with the consumption of the salt that supplements the nutrients, and adds 50 ml distilled waters, and by the 10th day, micro-algae density sum reached 1,800 ten thousand/milliliters, stopped cultivating, and carried out frustule collection;
The collection of the micro-algae of step 4
Preparing quick lime, copper sulphate, chitin, in quick lime: copper sulphate: chitin=1: the ratio mixing of 0.8: 2 (weight ratio), with mixer mix and blend 20 minutes, to even, make compound precipitants, get algae liquid described in step 3, the ratio adding 120 milligrams of compound precipitantses in 1 liter of algae liquid adds precipitating reagent, and glass bar stirs, and leaves standstill, precipitates 24 hours, frustule sinks to bottom and forms toothpaste-like algae cream, abandon supernatant, obtain algae cream, load in the polybag of dress food, insert-4 DEG C of Refrigerator stores, for subsequent use;
Step 5
The preparation bait formula of razor clam ecological bait: algae cream 28 parts, fish meal 0.6 part, blood meal 1.2 parts, cow dung 0.8 part, chicken manure 1.5 parts, chlorella powder 0.3 part, beer yeast powder 0.2 part, dried silkworm chrysalis meal 1 part, 0.1 part, peanut cake, 0.2 part, yellow meal worm powder, earthworm powder 0.7 part.Wherein powder particles size is 25-80 micron, and mixing and blending machine stirs, and bait completes, razor clam of can throwing something and feeding.
Prepare IBDU in nutrient solution process in step 1, oxamides, ammonium magnesium phosphate, APP, PA 800K, calcium polyphosphate potassium, ammonium sulfate, ammonium nitrate, the nutritive salt such as urea will add by the order provided in formula, to avoid chemical reaction;
In step 1, f/2 trace element solution and f/2 vitamin solution can be made into mother liquor in advance and be placed in 4 DEG C of refrigerator cold-storages and save backup, and do not exceed 30 days standing time;
Prepare micro algae culturing liquid IBDU used in step 1, oxamides, ammonium magnesium phosphate, APP, PA 800K, calcium polyphosphate potassium, ammonium sulfate, ammonium nitrate, urea, potassium sulfate, potassium chloride, the chemicals purity such as ammonium carbonate all need chemical pure rank;
Cultivate micro-algae magnetized drinking water preparation method used in step 1: after seawater boils, cooling, after flowing through water magnetizing appts, is magnetized drinking water.Water magnetizing appts magnetic induction intensity is 0.3T, and flow is 2t/h, and flow velocity is 0.3m/s;
The FeC in micro algae culturing liquid f/2 trace element solution used is prepared in step 1
6h
5o
75H
2o (ironic citrate), because of comparatively indissoluble solution, can add a small amount of running water low-grade fever on stove and, to 80-90 DEG C, and constantly be stirred to whole thawing;
Bait finished product should in ventilation, dry, low temperature place preservation;
Add the pure seawater (weight ratio) of 15-18 times during bait feeding and stir into scattered paste shape full pool spilling head to razor clam culturing pool.
Last it is noted that above embodiment is only in order to illustrate technical scheme of the present invention, be not intended to limit; Although with reference to previous embodiment to invention has been detailed description, those of ordinary skill in the art is to be understood that: it still can be modified to the technical scheme described in previous embodiment, or carries out equivalent replacement to wherein portion of techniques feature; And these amendments or replacement, do not make the essence of appropriate technical solution depart from the spirit and scope of embodiment of the present invention technical scheme.
Claims (1)
1. a preparation method for razor clam ecological bait, is characterized in that: the algae cream that this bait is made with five kinds of marine microalgaes, for main component, is aided with fish meal, blood meal, cow dung, chicken manure, chlorella powder, beer yeast powder, dried silkworm chrysalis meal, peanut cake, yellow meal worm powder, earthworm powder in proportion mixing manufacture forms, and concrete steps are as follows:
The preparation of step 1 micro algae culturing liquid
By weight: IBDU 0.3 part, oxamides 0.1 part, ammonium magnesium phosphate 0.02 part, APP 0.01 part, 0.05 part, calcium polyphosphate potassium, 0.02 part, ammonium sulfate, 0.04 part, ammonium nitrate, 0.1 part, urea, potassium sulfate 0.02 part, 0.6 part, potassium chloride, ammonium carbonate 8 parts, gibberellin 0.005 part, spermidine 0.008 part, f/2 trace element solution 1.2 parts, f/2 vitamin solution 1.2 parts, scalding and magnetization after 1000 parts, clean seawater; Acetic acid solution (volume ratio) with 15% and NaOH solution (weight ratio) adjust ph of 10% are to 7.8-8.2; Wherein f/2 trace element solution formula: ZnSO
44H
2o 23 milligrams, MnCl
24H
2o 178 milligrams, CuSO
45H
2o 10 milligrams, FeC
6h
5o
75H
2o 3.9 grams, Na
2moO
42H
2o 7.3 milligrams, CoCl
26H
2o 12 milligrams, Na
2eDTA 4.35 grams, distilled water 1000 milliliters; F/2 vitamin solution is filled a prescription: Cobastab
120.5 milligram, Cobastab
1100 milligrams, biotin 0.5 milligram, distilled water 1000 milliliters;
The one-level of the micro-algae of step 2 is cultivated
Get 1000 milliliters of circular flat bottom flasks, wash, add 200 ml distilled waters, electric furnace boils 3 minutes, sterilization, after outwell distilled water, cooling, add nutrient solution described in step 1 500 milliliters, inoculate respectively with rainbow rotary strainer algae that is pollution-free, pure, that be in exponential phase of growth, spot hailian seaweed, Skeletonema Costatum, tip boat-shaped algae, Phaeodactylum tricornutum, 5 kinds of micro-algae Mixed culture; Inoculum density is rainbow rotary strainer algae 2.5 ten thousand/milliliter respectively, spot hailian seaweed 30,000/milliliter, Skeletonema Costatum 1.5 ten thousand/milliliter, tip boat-shaped algae 40,000/milliliter, Phaeodactylum tricornutum 1.8 ten thousand/milliliter; Shake up gently, cover bottleneck with the clean newspaper of individual layer, rubber band ties, and puts in illumination box and cultivates; Do not inflate, every day manual shaking flask 6 times, each 3 minutes.First 3 days that cultivate, temperature was set to 18 DEG C, intensity of illumination 2500lux, light dark period L: D=12h: 12h, i.e. 12 h light, and 12 h dark, so circulate; The 4-6 days cultivated, temperature is adjusted to 24 DEG C, and intensity of illumination is set to 6000lux, and light dark period is set to L: D=14h: 10h, i.e. 14 h light, then 10 h dark, so moved in circles, by the 6th day, the growth of micro-algae reaches the peak of exponential phase of growth, density sum can reach 1,200 ten thousand/milliliters, stops cultivating, and proceeds to next stage and cultivates;
The secondary of the micro-algae of step 3 is cultivated
Get the bottle,suction of 2 3000 milliliters, after washing, use distilled water rinse again 3 times, the high mouth of first bottle is connected with emulsion tube or plastic tube with the low mouth of second bottle, junction does not leak gas, and each bottle adds the micro algae culturing liquid 1500 milliliters described in step 1, and the algae liquid after then cultivating by step 2 does seed liquor inoculation, inoculum concentration is 6: 1 (volume ratios), and namely 6 parts of nutrient solutions add 1 part of seed liquor; Then the low mouth of first bottle is connected with air compressor, and bottleneck rubber stopper clogs, and like this, the gas be filled with can obtain secondary and utilize; Be filled with mist, gas componant and volume ratio are V
air: V
purity oxygen: V
pure carbon dioxide: V
pure ammonia=97: 1.2: 1.3: 0.5, aeration quantity should not be too large, frustule can be allowed to suspend and do not precipitate; Between-line spacing is inflated, and namely inflates 1 hour, stops 1 hour, repeat; With green plastic film, bottle,suction is covered, irradiate the mainly green spectral of algae liquid like this; Be placed on microdisk electrode room by these 2 bottles to cultivate, culturing room is by air-conditioning temperature control, and fluorescent tube provides illumination, first 4 days that cultivate, set temperature 20 DEG C, intensity of illumination 3600lux, light dark period L: D=12h: 12h, 5-7 days, adjusts the temperature to 23 DEG C, intensity of illumination 4500lux, light dark period L: D=16h: 8h, i.e. illumination 16 hours, dark 8 hours, repeats; 8-10 days, adjust the temperature to 25 DEG C, intensity of illumination 9000lux, whole day is set to illumination, does not have dark; By the 8th day, the nutrient solution 100 milliliters described in step 1 added by each bottle, with the consumption of the salt that supplements the nutrients, and adds 50 ml distilled waters, and by the 10th day, micro-algae density sum reached 1,800 ten thousand/milliliters, stopped cultivating, and carried out frustule collection;
The collection of the micro-algae of step 4
Preparing quick lime, copper sulphate, chitin, in quick lime: copper sulphate: chitin=1: the ratio mixing of 0.8: 2 (weight ratio), with mixer mix and blend 20 minutes, to even, make compound precipitants, get algae liquid described in step 3, the ratio adding 120 milligrams of compound precipitantses in 1 liter of algae liquid adds precipitating reagent, and glass bar stirs, and leaves standstill, precipitates 24 hours, frustule sinks to bottom and forms toothpaste-like algae cream, abandon supernatant, obtain algae cream, load in the polybag of dress food, insert-4 DEG C of Refrigerator stores, for subsequent use;
Step 5
The preparation bait formula of razor clam ecological bait: algae cream 28 parts, fish meal 0.6 part, blood meal 1.2 parts, cow dung 0.8 part, chicken manure 1.5 parts, chlorella powder 0.3 part, beer yeast powder 0.2 part, dried silkworm chrysalis meal 1 part, 0.1 part, peanut cake, 0.2 part, yellow meal worm powder, earthworm powder 0.7 part.Wherein powder particles size is 25-80 micron, and mixing and blending machine stirs, and bait completes, razor clam of can throwing something and feeding.
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| CN105533292A (en) * | 2016-03-08 | 2016-05-04 | 临沂大学 | Culture method of bait microalgae for D-shaped larvae of Scapharca broughtonii |
| CN107900097A (en) * | 2017-11-14 | 2018-04-13 | 中国石油大学(北京) | Nitrogen and phosphorus sustained release agent and preparation method and the application in remedying oil-polluted intertidal zone |
| CN114164117A (en) * | 2021-12-31 | 2022-03-11 | 宁波浮田生物技术有限公司 | Culture medium suitable for bait microalgae, culture method and application |
| CN115956639A (en) * | 2023-01-10 | 2023-04-14 | 东北林业大学 | Feed for freshwater mussel breeding |
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