CN104789450B - Connecting device for fermented container and extracellular fermented product researching container - Google Patents
Connecting device for fermented container and extracellular fermented product researching container Download PDFInfo
- Publication number
- CN104789450B CN104789450B CN201510259352.3A CN201510259352A CN104789450B CN 104789450 B CN104789450 B CN 104789450B CN 201510259352 A CN201510259352 A CN 201510259352A CN 104789450 B CN104789450 B CN 104789450B
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- pipe
- attachment means
- container
- tube
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/08—Flask, bottle or test tube
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M43/00—Combinations of bioreactors or fermenters with other apparatus
Abstract
The invention discloses a connecting device for a fermented container and an extracellular fermented product researching container. The fermented container is a flat bottom flask with an aeration device and a stirring device, wherein a connecting pipe is arranged at the bottom of the flat bottom flask; the extracellular fermented product researching container is a test tube with an aeration device, wherein a connecting pipe is arranged at the bottom of the test tube; the connecting device is composed of a freely detachable glass sleeve coaxially melted with a G1 sand chip, coaxially arranged regenerated cellulose membrane, an O-shaped silica gel gasket and an inserting pipe inserted into the glass sleeve; the cross section at the bottom end of the inserting pipe is closely contacted with the O-shaped silica gel gasket; the G1 sand chip, the regenerated cellulose membrane and the O-shaped silica gel gasket are closely contacted by squeezing the cross section of the bottom end of the inserting tube; extending pipes with screw or dull polish sorted with the flat bottom flask and the bottom of the test tube are arranged at two ends of the device. The device provided by the invention is simple and rapid in assembly, freely detachable, convenient to use and is widely applied in an extracellular active component system for connecting the fermentation and researching the fermentation generation.
Description
Technical field
The present invention relates to a kind of fermentation and the attachment means of Study on product system thereof, particularly relate to a kind of outside round with born of the same parents
The attachment means of tunning research container.
Background technology
It is active component (albumen, the polysaccharide this fermentation produced that existing research has the method for given activity fermentation system
Or small-molecule substance etc.) separate from the extracellular fluid of fermentation, it is purified.The defect of this research method is to ignore fermentation body
Microorganism or the effect of cell itself in system, it is impossible to feed active substance, particularly some active components continuously and very likely dividing
Activity is lost during from.Additionally research has the method for given activity fermentation system is active component in direct research system, but
Because fermentation system extracellular fraction is complicated, the activity of some component meeting direct interference target components.Continuous in order to realize fermentation system
Supply active component, gets rid of the interference of the outer non-target components of born of the same parents, saves the purification procedures of complexity simultaneously, and exploitation one is used for
Round and the attachment means of cell free fermentation Study on product container, select the active component in the outer system of complicated born of the same parents of fermentation generation
Select and penetrate into that directly to carry out character research in research system the most necessary and significant.But find relevant described company through retrieval
Connection device and pertinent literature have no report.
Summary of the invention
For the deficiency of existing research method, it is an object of the invention to provide one and grind with cell free fermentation product for round
Study carefully the attachment means of container.
In order to achieve the above object, the technical solution used in the present invention is:
Attachment means for round Yu cell free fermentation Study on product container of the present invention, described round is band ventilation
The bottom of device and agitating device is provided with the boiling flask of connecting tube, and described extracellular products research container is the end of band breather
Portion is provided with the test tube of connecting tube, and wherein said breather includes air inlet pipe, escape pipe and aseptic air filter, described in stir
Mixing device and include magnetic stir bar and magnetic agitation oar, bottom described boiling flask, external diameter and the internal diameter of connecting tube are equal to bottom test tube
The external diameter of connecting tube and internal diameter and two connecting tubes are coaxial, bottom connecting tube outer wall bottom boiling flask or test tube the height of extended line
Degree is for 2-3mm, and connecting tube one end, bottom is connected with respective container, the other end with screw socket or ground form and attachment means its
The extension compact siro spinning technology of middle one end, described attachment means two ends are provided with extension and seam are provided with ground folder；
It is characterized in that: described attachment means by the coaxial molten glass bushing being embedded with G1 core sheet that can freely dismantle and is total to successively
The regenerated cellulose diaphragm that axle is laid, " O " type silicone gasket and the insertion pipe composition inserted in glass bushing, wherein insert pipe
Cross section, bottom is in close contact with " O " type silicone gasket, and G1 core sheet, regenerated cellulose diaphragm and " O " type silicone gasket
By inserting pipe bottom sectional crush compact siro spinning technology；Described attachment means two ends be provided with boiling flask bottom at the bottom of connecting tube and test tube
The extension of the threaded or frosted of the supporting connection of portion's connecting tube；Described G1 sand chip thickness is 4-6mm, outside distance glass bushing
Pipe in frosted inclined plane bottom 0.6-2mm, its diameter of section equal with glass bushing inner tube diameter and with glass bushing inner tube top
Cross section is coaxial coplanar；Described " O " type silicone gasket is plain washer, and its external diameter is equal with inserting pipe bottom face external profile diameter, interior
Footpath is equal with inserting the interior center-hole diameter coaxial with inserting pipe of pipe bottom face, and the ratio G1 core little 4-5mm of sheet diameter, its thickness
For 0.4-0.6mm；The regenerated cellulose diaphragm diameter arranged between G1 core sheet and " O " type silicone gasket is equal in glass bushing
Pipe external diameter, its molecular weight ranges that can retain is 100-300KDa；Insert pipe outer wall and have supporting with frosted in glass bushing outer tube
Outer frosted, outer frosted inclined plane lower edge has the non-ground glass district of 0.15-1.45mm length range.
In the above-mentioned attachment means for round with cell free fermentation Study on product container: described regenerated cellulose diaphragm can retain
Molecular weight ranges be preferably: 100-500Da, 500-1000Da, 1000Da, 2000Da, 3500Da, 3500-5000Da,
8KDa, 8K-10KDa, 10KDa, 20KDa, 50KDa, 100KDa or 300KDa.
Above-mentioned in the attachment means of round and cell free fermentation Study on product container: according to connecting tube bottom boiling flask with
The amplification of connecting tube quantity bottom test tube, described attachment means quantity can corresponding supporting extension.
Attachment means for round and cell free fermentation Study on product container of the present invention be connected fermentation and research this
Application in the outer active component system of the born of the same parents that ferment produces.
The invention has the beneficial effects as follows:
Attachment means for round with cell free fermentation Study on product container the most of the present invention assembles simple and fast, can be certainly
By dismantling, application is convenient.
The application of attachment means the most of the present invention eliminates the purification procedures complicated to target extracellular fraction.
3. can the non-target components interference to target substance measurement result in the outer system of the born of the same parents to a certain degree avoiding fermentation to produce.
4., when using the supporting connection of many body system, several active components in born of the same parents' external system that fermentation produces can be studied simultaneously.
5. can avoid omitting have synergistic not clear component with main research material.
6. can study the active component in born of the same parents' external system that fermentation produces continuously.
Accompanying drawing explanation
Fig. 1 attachment means of the present invention structural representation.
The concrete device of active component system outer with the born of the same parents studying this fermentation generation that the attachment means of Fig. 2 present invention connects fermentation shows
It is intended to.
Fig. 3 Double bottom portion connects the schematic diagram of container application attachment means of the present invention.
In above-mentioned Fig. 1～Fig. 3: 1. glass bushing, 2. glass bushing outer tube, 3. glass bushing inner tube, 4. outside glass bushing
Tube wall, 5. glass bushing inner tubal wall, 6.G1 core sheet, 7. glass bushing inner tube tip section, 8.G1 core sheet tip section,
9. glass bushing outer tube frosted sloped bottom edges cross section, 10. regenerated cellulose diaphragm, 11. " O " type silicone gasket, 12. insert pipe
Cross section, bottom, 13. insert Guan Fei ground glass district, frosted in 14. glass bushing outer tubes, and 15. insert the outer frosted of pipe, and 16. insert
Pipe, 17. extensions, 18. screw threads, 19. ground folders, 20. screw socket junctions, the attachment means shown in 21. Fig. 1,22. ends
The boiling flask (round) of portion's band connecting tube, the test tube (research container) of 23. bottom belt connecting tubes, 24. air inlet pipe, 25.
Magnetic stir bar, 26. magnetic agitation oars, 27. disc type respiratory organs or title aseptic air filter, 28. rubber stoppers, 29. give vent to anger
Pipe, connecting tube bottom 30. boiling flasks or test tube.
Detailed description of the invention
Below in conjunction with the accompanying drawings the attachment means for round with cell free fermentation Study on product container of the present invention is entered
One step elaborates.
Embodiment 1
Regenerated cellulose diaphragm (10) that the molecular weight that can retain is 5KDa is pressed in G1 core sheet (6) tip section (8)
On, compress " O " type silicone gasket (11), insertion pipe (16) is inserted in glass bushing (1), insert pipe bottom and cut
" O " type silicone gasket that the extruding of face (12) consolidation ground is placed in glass bushing makes " O " type silicone gasket (11), regeneration
Cellulose diaphragm (10) and G1 core sheet (6) compact siro spinning technology, insert pipe (16) and glass bushing (1) in assembling process
By frosted in glass bushing outer tube (14) and insertion pipe outer frosted (15) compact siro spinning technology, ground folder (19) clamping seam
(as shown in Figure 2) so that attachment means shown in Fig. 1 (21) compact siro spinning technology, i.e. obtain of the present invention for round
Attachment means (as shown in Figure 1) with cell free fermentation Study on product container.
Embodiment 2
Regenerated cellulose diaphragm (10) that the molecular weight that can retain is 1KDa is pressed in G1 core sheet (6) tip section (8)
On, compress " O " type silicone gasket (11), insertion pipe (16) is inserted in glass bushing (1), insert pipe bottom and cut
" O " type silicone gasket that the extruding of face (12) consolidation ground is placed in glass bushing makes " O " type silicone gasket (11), regeneration
Cellulose diaphragm (10) and G1 core sheet (6) compact siro spinning technology, insert pipe (16) and glass bushing (1) in assembling process
By frosted in glass bushing outer tube (14) and insertion pipe outer frosted (15) compact siro spinning technology, ground folder (19) clamps Fig. 2 institute
The seam shown so that attachment means shown in Fig. 1 (21) compact siro spinning technology.
Again by the attachment means (21) shown in Fig. 1 by the screw thread (18) at its extension (17) two ends and boiling flask (22)
Corresponding screw thread upper with test tube (23) bottom connecting tube (30) is at screw socket junction (20) spiral compact siro spinning technology as shown in Figure 2.
Boiling flask (22) covers band agitating device (magnetic stir bar (25)+magnetic agitation oar (26)) and breather (enters
Trachea (24)+disc type respiratory organ (27)+escape pipe (29)) rubber stopper (28).Band breather (dish is covered on test tube (23)
Formula respiratory organ (27)+escape pipe (29)) rubber stopper (28).Whole device is as shown in Figure 2.
The attachment means for round Yu cell free fermentation Study on product container of above-mentioned assembling, described round is band ventilation
The bottom of device and agitating device is provided with the boiling flask of connecting tube, and described extracellular products research container is the end of band breather
Portion is provided with the test tube of connecting tube, and wherein said breather includes air inlet pipe, escape pipe and aseptic air filter, described in stir
Mixing device and include magnetic stir bar and magnetic agitation oar, bottom described boiling flask, external diameter and the internal diameter of connecting tube are equal to bottom test tube
The external diameter of connecting tube and internal diameter and two connecting tubes are coaxial, bottom connecting tube outer wall bottom boiling flask or test tube the height of extended line
Degree is 3mm, and connecting tube one end, bottom is connected with respective container, and the other end is with screw socket form and attachment means wherein one end
Extension compact siro spinning technology, described attachment means two ends are provided with extension and seam are provided with ground folder；Wherein, described company
Connection device is embedded with the glass bushing of G1 core sheet and the most coaxial regenerated cellulose diaphragm laid by coaxial the melting that can freely dismantle,
" O " type silicone gasket and the insertion pipe composition inserted in glass bushing, wherein insert cross section, pipe bottom and " O " type silicone gasket
It is in close contact, and G1 core sheet, regenerated cellulose diaphragm and " O " type silicone gasket are closely connected by inserting pipe bottom sectional crush
Connect；Described attachment means two ends be provided with supporting with connecting tube bottom connecting tube bottom boiling flask and test tube be connected threaded
Extension；Described G1 sand chip thickness is 5mm, frosted inclined plane bottom 2mm in distance glass bushing outer tube, its diameter of section
Equal with glass bushing inner tube diameter and coaxial coplanar with glass bushing inner tube tip section；Described " O " type silicone gasket is flat
Packing ring, its external diameter is equal with inserting pipe bottom face external profile diameter, and internal diameter manages coaxial centre bore with inserting in inserting pipe bottom face
Equal diameters, and the ratio G1 core little 4mm of sheet diameter, its thickness is 0.5mm；Set between G1 core sheet and " O " type silicone gasket
The regenerated cellulose diaphragm diameter put is equal to glass bushing inner tube external diameter；Insertion pipe outer wall has joins with frosted in glass bushing outer tube
The outer frosted of set, outer frosted inclined plane lower edge has the non-ground glass district of 1.45mm.
By package unit high pressure wet-hot steam sterilizing as shown in Figure 2, after end device subject to sterilization cooling, in aseptic technique
Under, boiling flask (22) is poured into the kirk liquid synthesis of the most sterilized Phanerochaete chrysosporium
Culture medium, Phanerochaete chrysosporium spore suspension prepared by inoculation PDA culture medium preculture, in examination
Pipe (23) is poured the aseptic lignin solution with the synthesis culture fluid preparation of sterilizing kirk liquid, during then entirety puts into incubator into
Implement to cultivate.
The molecular weight that in incubation, Phanerochaete chrysosporium secretion the produces component less than 1KDa is due to dense
Degree gradient can retain 1KDa by of the present invention in the attachment means of round and cell free fermentation Study on product container
Molecular weight membrane diffusion enters test tube.
Before sampling, the material in test tube is mixed, from test tube, take the sample of partial volume, with 1ml 5M NaOH, pH is adjusted
To 13, centrifugal 13900rpm, 4 DEG C, 10min, take the super filter tube 4000g of supernatant 5k, 4 DEG C, be centrifuged 30min.
The material mixing taken on super filter tube in pipe, measures with gel permeation chromatography (GPC) > molecular weight of the lignin part of 5kDa
Distribution.
Take off pipe < part LC-MS (LC-MS) detection of 5kDa.
Judged the degraded situation of lignin by the result of GPC and LC-MS detection, thus judge that whether active component is less than 1KDa
Molecular weight ranges in.
Continuous sampling detects, and checks the change of the active substance xylogen degradation ability that Phanerochaete chrysosporium secretes
Change situation.
Embodiment 3
Regenerated cellulose diaphragm (10) that the molecular weight that can retain is 1KDa and 5KDa is press respectively against two set glass bushings (1)
G1 core sheet (6) tip section (8) on, then press two " O " type silicone gaskets (11) respectively, by two insertions
Pipe (16) inserts in two glass bushings (1) respectively, inserts the extruding of cross section, pipe bottom (12) consolidation ground and is placed on glass sock
" O " type silicone gasket in pipe, makes " O " type silicone gasket (11), regenerated cellulose diaphragm (10) and G1 core sheet (6)
Compact siro spinning technology, inserts pipe (16) and glass bushing (1) and by frosted (14) in glass bushing outer tube and inserts the outer frosted of pipe
(15) compact siro spinning technology, two grounds folder (19) clamp seam as shown in Figure 3 respectively so that two set of connection devices (21)
Compact siro spinning technology.
Again by the attachment means (21) shown in two set Fig. 1 by the screw thread (18) at its extension (17) two ends and bottom with
The boiling flask (22) of two connecting tubes and two bottoms with connecting tube test tube (23) by corresponding screw thread at screw socket even
Connect place (20) spiral compact siro spinning technology as shown in Figure 3.
Boiling flask (22) covers band agitating device (magnetic stir bar (25)+magnetic agitation oar (26)) and breather (enters
Trachea (24)+disc type respiratory organ (27)+escape pipe (29)) rubber stopper (28).Band is covered logical on two test tubes (23)
The rubber stopper (28) of device of air (disc type respiratory organ (27)+escape pipe (29)).Whole device is as shown in Figure 3.
By package unit high pressure wet-hot steam sterilizing as shown in Figure 3, after end device subject to sterilization cooling, in aseptic technique
Under, boiling flask (22) is poured into the kirk liquid synthesis of the most sterilized Phanerochaete chrysosporium
Culture medium, Phanerochaete chrysosporium spore suspension prepared by inoculation PDA culture medium preculture, in examination
Pipe (23) is poured the aseptic lignin solution with the synthesis culture fluid preparation of sterilizing kirk liquid, during then entirety puts into incubator into
Implement to cultivate.
The molecular weight that in incubation, Phanerochaete chrysosporium secretion produces is less than the component of 1KDa and is less than
The molecule of 5KDa overlaps the company for round with cell free fermentation Study on product container due to Concentraton gradient by of the present invention two
The membrane diffusion that can retain 1KDa in connection device respectively and retain 5KDa respectively enters two corresponding test tubes.
Before sampling, the material in test tube is mixed, from test tube, take the sample of partial volume, with 1ml 5M NaOH, pH is adjusted
To 13, centrifugal 13900rpm, 10 DEG C, 10min, take the super filter tube 4000g of supernatant 5k, 4 DEG C, be centrifuged 30min.
The material mixing taken on super filter tube in pipe, measures with gel permeation chromatography (GPC) > molecular weight of the lignin part of 5kDa
Distribution.
Take off pipe < part LC-MS (LC-MS) detection of 5kDa.Sentenced by GPC and LC-MS sampling test result
Determine the degraded situation of lignin, thus judge that active component is whether more than in the 1KDa molecular weight ranges less than 5KDa.
Continuous sampling detects, and checks the outer active substance xylogen degradation ability of born of the same parents that Phanerochaete chrysosporium secretes
Situation of change.
Change the regenerated cellulose film of less scope, repeat the above steps, reduce Phanerochaete chrysosporium
The molecular weight ranges of the outer xylogen degradation active component of the born of the same parents of secretion.
Claims (4)
1., for an attachment means for round with cell free fermentation Study on product container, described round is band breather
With the boiling flask that the bottom of agitating device is provided with connecting tube, described extracellular products research container is that the bottom of band breather sets
Being equipped with the test tube of connecting tube, the breather of wherein said round includes air inlet pipe, escape pipe and aseptic air filter,
Described agitating device includes magnetic stir bar and magnetic agitation oar, and bottom described boiling flask, external diameter and the internal diameter of connecting tube are equal to examination
Bottom pipe, the external diameter of connecting tube and internal diameter and two connecting tubes are coaxial, extend bottom connecting tube outer wall bottom boiling flask or test tube
The height of line is 2-3mm, and connecting tube one end, bottom is connected with respective container, the other end with screw socket or ground form be connected
The extension compact siro spinning technology of device wherein one end, described attachment means two ends are provided with extension and seam are provided with ground
Folder；
It is characterized in that: described attachment means by the coaxial molten glass bushing being embedded with G1 core sheet that can freely dismantle and is total to successively
The regenerated cellulose diaphragm that axle is laid, " O " type silicone gasket and the insertion pipe composition inserted in glass bushing, wherein insert at the bottom of pipe
End section is in close contact with " O " type silicone gasket, and G1 core sheet, regenerated cellulose diaphragm and " O " type silicone gasket are by inserting
Pipe bottom sectional crush compact siro spinning technology；Described attachment means two ends are provided with and are connected bottom connecting tube bottom boiling flask and test tube
Manage the extension of the threaded of supporting connection or frosted；Described G1 sand chip thickness is 4-6mm, in distance glass bushing outer tube
Frosted inclined plane bottom 0.6-2mm, its diameter of section equal with glass bushing inner tube diameter and with glass bushing inner tube tip section
Coaxial coplanar；Described " O " type silicone gasket is plain washer, and its external diameter is equal with inserting pipe bottom face external profile diameter, and internal diameter is with slotting
Center-hole diameter coaxial with inserting pipe in entering pipe bottom face is equal, and the ratio G1 core little 4-5mm of sheet diameter, and its thickness is
0.4-0.6mm；The regenerated cellulose diaphragm diameter arranged between G1 core sheet and " O " type silicone gasket is equal to outside glass bushing inner tube
Footpath, its molecular weight ranges that can retain is 100Da-300KDa；Insert pipe outer wall and have supporting with frosted in glass bushing outer tube
Outer frosted, outer frosted inclined plane lower edge has the non-ground glass district of 0.15-1.45mm length range.
The most according to claim 1 for the attachment means of round with cell free fermentation Study on product container, it is characterised in that:
The molecular weight ranges that described regenerated cellulose diaphragm can retain is: 100-500Da, 500-1000Da, 1000Da, 2000Da,
3500Da, 3500-5000Da, 8KDa, 8K-10KDa, 10KDa, 20KDa, 50KDa, 100KDa or 300KDa.
The most according to claim 1 for the attachment means of round with cell free fermentation Study on product container, it is characterised in that:
According to the amplification of connecting tube quantity bottom connecting tube bottom boiling flask and test tube, described attachment means quantity can corresponding supporting expansion
Exhibition.
4. it is being connected fermentation and research for round with the attachment means of cell free fermentation Study on product container described in claim 1
Application in the outer active component system of the born of the same parents that this fermentation produces.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510259352.3A CN104789450B (en) | 2015-05-19 | 2015-05-19 | Connecting device for fermented container and extracellular fermented product researching container |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510259352.3A CN104789450B (en) | 2015-05-19 | 2015-05-19 | Connecting device for fermented container and extracellular fermented product researching container |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104789450A CN104789450A (en) | 2015-07-22 |
| CN104789450B true CN104789450B (en) | 2017-01-11 |
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| CN201510259352.3A Expired - Fee Related CN104789450B (en) | 2015-05-19 | 2015-05-19 | Connecting device for fermented container and extracellular fermented product researching container |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201634664U (en) * | 2009-12-23 | 2010-11-17 | 罗建峰 | Sampling device on grape wine fermentation tank |
| CN102112594A (en) * | 2008-08-01 | 2011-06-29 | 智能生物系统公司 | A sample port of a cell culture system |
| CN103952296A (en) * | 2014-01-14 | 2014-07-30 | 卢建熙 | Multifunctional medical treatment substance body combiner |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20060014260A1 (en) * | 2004-05-07 | 2006-01-19 | Zhiliang Fan | Lower cellulase requirements for biomass cellulose hydrolysis and fermentation |
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2015
- 2015-05-19 CN CN201510259352.3A patent/CN104789450B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102112594A (en) * | 2008-08-01 | 2011-06-29 | 智能生物系统公司 | A sample port of a cell culture system |
| CN201634664U (en) * | 2009-12-23 | 2010-11-17 | 罗建峰 | Sampling device on grape wine fermentation tank |
| CN103952296A (en) * | 2014-01-14 | 2014-07-30 | 卢建熙 | Multifunctional medical treatment substance body combiner |
Non-Patent Citations (1)
| Title |
|---|
| H-2过滤装置的制作与应用;黄雪斐，洪碧君，张勤;《护理学杂志》;20010531;第16卷(第5期);278-280 * |
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