CN104782663B - A kind of microbial insecticide composition for preventing and treating red fire ant - Google Patents
A kind of microbial insecticide composition for preventing and treating red fire ant Download PDFInfo
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- CN104782663B CN104782663B CN201510103305.XA CN201510103305A CN104782663B CN 104782663 B CN104782663 B CN 104782663B CN 201510103305 A CN201510103305 A CN 201510103305A CN 104782663 B CN104782663 B CN 104782663B
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Abstract
The invention discloses a kind of microbial insecticide composition for preventing and treating red fire ant.It includes muscardine and thiacloprid, and the number of beauveria bassiana spore and the grams ratio of thiacloprid are：(8~1) × 20,000,000,000：2~9g.The present invention is found by experiment that, muscardine and thiacloprid compounding have significant synergistic effect, prevention effect is very good, there is efficient, quick advantage to red fire ant, it is nontoxic to people and animals, it is free of contamination to soil and groundwater resources, and its safety, so as to both improve the insecticidal effect of muscardine, delay the drug-fast enhancing of red fire ant again, and the usage amount of chemical pesticide thiacloprid can be reduced, environmental pollution is reduced, so as to improve preventive effect, the consumption of active ingredient is reduced, it is cost-effective, delay the generation of pest resistance to insecticide.
Description
Technical field：
The invention belongs to Solenopsis invicta field, and in particular to a kind of microbial insecticide composition of preventing and treating red fire ant.
Background technology：
Muscardine is that a more class insect pathogenic fungus is applied in countries in the world research, can infect the section of 15 mesh 149 kind more than 700
Insect (Feng et al., 1994).It is external to carry out the research that application muscardine prevents and treats red fire ant already, Stimac etc. (1993)
Successively various concentrations are have studied with field indoors respectively with Oi etc. (1994), and the muscardine of different administration method is right in ant nest
The control situation of red fire ant.Therefore disease fungus provides hope (Liu Xiaoyan etc., 2006) for the sustainable control of red fire ant.Together
When muscardine be considered as one of more promising pathogenic microorganism.
Thiacloprid：The mechanism of action of thiacloprid and other conventional pesticides are different.It mainly acts on insect nerve
Caudacoria is engaged, by being combined with nAChR, interference insect nervous system normal conduction causes the resistance of neural channel
Plug, causes a large amount of accumulation of acetylcholine, so that insect exaltation, general spasticity, paralysis and it is dead.In stronger
Inhale, tag and stomach poison function, do not interacted with common insecticides such as pyrethroid, organic phosphates and carbamates anti-
Property, thus available for resistance management.Thiacloprid has very high security to people and animals, and medicament is destitute of smell or excitant,
To dispenser operating personnel and applying area resident safety, and warding off property of drive will not be caused to red fire ant.In addition, thiacloprid is pacified to environment
Entirely.Because the steam of its active ingredient is forced down, thiacloprid will not pollute air.Due to half-life short, the residual matter of thiacloprid is entered to bury
Influence very little also rapid decomposable after earth and river, being caused to environment.In addition, thiacloprid is to fish and other aquatiles
Toxicity is also very low, there is no influence on aquatile under normal circumstances.
Often there are different journeys in a kind of preventing and treating for the red fire ant that nest is done in soil containing insecticide of active component
The defect of degree：It is continuous easily to be developed immunity to drugs using insect during chemical pesticide, and using the stronger chemical pesticide of toxicity or apply
With method is improper the nest of removing of red fire ant, or even prevention and control medicament can be caused to cause serious dirt to soil and groundwater resources
Dye.In addition, the speed that pathogenic microorganism muscardine preventing and treating red fire ant is administered alone is relatively slow.
So far there are no report that insecticide that muscardine combines with thiacloprid applies on prevention and control red fire ant.
The content of the invention：
It is an object of the invention to provide a kind of microorganism of efficient, safely and quickly killing red ant preventing and treating red fire ant
Insecticides.
The inventors discovered that, by muscardine and thiacloprid compounding after, it has synergistic effect, to red fire ant have efficiently,
Quick advantage, it is nontoxic to people and animals, it is free of contamination to soil and groundwater resources, and its safety, so as to both improve muscardine
Insecticidal effect, delayed the drug-fast enhancing of red fire ant again.
The microbial insecticide composition of the preventing and treating red fire ant of the present invention, it is characterised in that including muscardine and thiacloprid,
The number of beauveria bassiana spore and the grams ratio of thiacloprid are：(8~1) × 20,000,000,000：2~9g.
It is preferred that, the number of beauveria bassiana spore and the grams ratio of thiacloprid are：8 × 20,000,000,000：2g.
The microbial insecticide composition of the preventing and treating red fire ant of the present invention, can also add conventional use of assistant agent, and make
Into different formulations, preferably water-dispersible granules, based on mass fraction 100%, including muscardine pulvis and thiacloprid is total
Mass fraction is 30%, and muscardine pulvis and thiacloprid mass ratio are 8~1：2~9, described muscardine pulvis contains muscardine
20,000,000,000/g of spore, wetting agent pull open powder 8%, dispersant sodium lignin sulfonate 18%, disintegrant calcium chloride 5%, stabilizer phosphorus
Sour disodium hydrogen 2%, adhesive Sodium Polyacrylate 5% and carrier diatomaceous earth 32%.
The present invention is found by experiment that muscardine and thiacloprid compounding have significant synergistic effect, and prevention effect is very good,
There is efficient, quick advantage to red fire ant, it is nontoxic to people and animals, it is free of contamination to soil and groundwater resources, and its safety, from
And the insecticidal effect of muscardine had both been improved, delay the drug-fast enhancing of red fire ant again, and chemical pesticide thiacloprid can be reduced
Usage amount, reduce environmental pollution, so as to improve preventive effect, reduce the consumption of active ingredient, it is cost-effective, delay pest resistance to insecticide
Generation.
Embodiment：
Following examples are that the present invention is further illustrated, rather than limitation of the present invention.
Embodiment 1：Muscardine compounds host with thiacloprid and the Toxicity Determination of red fire ant worker ant is tested
During Toxicity Determination, weigh muscardine pulvis and thiacloprid amounts to 1.5g (wherein muscardine pulvis and thiacloprid
Mass ratio is respectively：1：0、8：2、6：4、5：5、4：6、1：9 and 0：1, muscardine pulvis used containing beauveria bassiana spore 20,000,000,000/
G, described thiacloprid active compound content is mass fraction 95%, when actual preparation is calculated with muscardine quality ratio, with pure thiacloprid
To calculate), wetting agent pull open powder 0.4g, dispersant sodium lignin sulfonate 0.9g, disintegrant calcium chloride 0.25g, stabilizer phosphoric acid
Disodium hydrogen 0.1g, adhesive Sodium Polyacrylate 0.25g, carrier diatomaceous earth 1.6g.Above raw material presses the preparation of water-dispersible granules
Method input mixing (1, according to formula raw material is well mixed；2nd, it is sanded after being well mixed through sand mill and detects qualified laggard
Row mist projection granulating；3rd, sieved after mist projection granulating by vibratory sieve, profile defective work returns to sand mill and is sanded again, outside
Shape certified products further quality testing, if product off quality also return to sand mill and are sanded again；4th, up-to-standard product are detected,
Packed；5th, the packaged water dispersible granules are put in storage), the muscardine thiacloprid water of mass fraction 30% is finally made
Dispersible granule (the microbial insecticide composition of preventing and treating red fire ant).
Virulence of the above-mentioned muscardine thiacloprid water dispersible granules to healthy red fire ant worker ant is determined respectively.Each processing 3
Secondary repetition, each repeats 1 culture dish, and each culture dish puts 100 red fire ant worker ants.Red fire ant worker ant is immersed into decoction first
Take out, be put into after drying indoors in rearing-box after (each medicine to be measured diluted with the water of 100 times of its quality after solution) about 5s,
Raise daily with fresh honey, be placed in 26 ± 1 DEG C, humidity 70-90% is raised under the conditions of photoperiod 14L/10hD, in 72h after medicine
The survival condition of red fire ant worker ant is observed, the death rate, the statistics lethal concentration of 50 (LC is calculated50).Calculated altogether according to Sun methods (1960)
Malicious coefficient (CTC).When co-toxicity coefficient CTC is less than 80, there is decreasing effect effect therebetween；There is synergistic effect during more than 120；Between
It is summation action when between 80-120.
The toxicity test result of each single dose of table 1 and its mixture to red fire ant worker ant 72h
| Muscardine：Thiacloprid (w/w) | LC50(mg/kg) | ATI | TTI | Co-toxicity coefficient (CTC) |
| 1:0 | 6.55 | 100 | - | - |
| 0:1 | 0.81 | 808.64 | - | - |
| 8：2 | 0.76 | 861.84 | 241.73 | 356.53 |
| 6：4 | 0.68 | 963.24 | 383.46 | 251.20 |
| 5：5 | 0.79 | 829.11 | 454.32 | 182.50 |
| 4：6 | 0.58 | 1129.31 | 525.19 | 215.03 |
| 1：9 | 0.62 | 1056.45 | 737.78 | 143.19 |
As shown in table 1, from table 1, muscardine pulvis is with thiacloprid with mass ratio 8 for concrete outcome：2、6：4、5：5、4：
6、1：When 9 ratios are mixed, its co-toxicity coefficient is up to 356.53,251.20,182.50,215.03,143.19, with synergistic effect.
Especially muscardine pulvis and thiacloprid is with mass ratio 8：During 2 mixing, its co-toxicity coefficient is up to 356.53, and synergistic effect shows very much
Write.
Embodiment 2：
Prepare 30% muscardine thiacloprid (thiacloprid of 1.2% muscardine+0.3%) water dispersible granules, the matter of each component
Amount is respectively：Weigh muscardine pulvis 1.2g, thiacloprid 0.3g, wetting agent pull open powder 0.4g, dispersant sodium lignin sulfonate
0.9g, disintegrant calcium chloride 0.25g, stabilizer disodium hydrogen phosphate 0.1g, adhesive Sodium Polyacrylate 0.25g, carrier diatomaceous earth
1.6g.30% muscardine thiophene is made by preparation method (with reference to embodiment 1) input mixing of water-dispersible granules in above raw material
Worm quinoline water dispersible granules (the microbial insecticide composition of preventing and treating red fire ant).Muscardine pulvis used contains beauveria bassiana spore 200
Hundred million/gram, thiacloprid active compound content is 95%, when actual preparation is calculated with muscardine quality ratio, is calculated with pure thiacloprid.Should
The microbial insecticide composition for preventing and treating red fire ant dilutes 72 hours inspection drug effects after spraying, medicine by 100-150 times.The preventing and treating is red
The microbial insecticide composition of fiery ant is very good to the prevention effect of red fire ant, has efficient, quick advantage to red fire ant.
Embodiment 3：
30% muscardine thiacloprid (thiacloprid of 0.75% muscardine+0.75%) water dispersible granules are prepared, each component
Quality is respectively：Weigh muscardine pulvis 0.75g, thiacloprid 0.75g, wetting agent pull open powder 0.4g, dispersant lignin sulfonic acid
Sodium 0.9g, disintegrant calcium chloride 0.25g, stabilizer disodium hydrogen phosphate 0.1g, adhesive Sodium Polyacrylate 0.25g, carrier diatomaceous
Native 1.6g.30% muscardine is made by the preparation method input mixing (with reference to embodiment 1) of water-dispersible granules in above raw material
Thiacloprid water dispersible granules.Muscardine pulvis used/gram containing beauveria bassiana spore 20,000,000,000, thiacloprid active compound content is 95%, real
When calculating is prepared with muscardine quality ratio in border, calculated with pure thiacloprid.The microbial insecticide composition of the preventing and treating red fire ant
Diluted 72 hours after spraying, medicine by 100-150 times and check drug effect.The microbial insecticide composition of the preventing and treating red fire ant is to flourishing
The prevention effect of ant is very good, has efficient, quick advantage to red fire ant.
Embodiment 4：
30% muscardine thiacloprid (thiacloprid of 0.15% muscardine+1.35%) water dispersible granules are prepared, each component
Quality is respectively：Weigh muscardine pulvis 0.15g, thiacloprid 1.35g, wetting agent pull open powder 0.4g, dispersant lignin sulfonic acid
Sodium 0.9g, disintegrant calcium chloride 0.25g, stabilizer disodium hydrogen phosphate 0.1g, adhesive Sodium Polyacrylate 0.25g, carrier diatomaceous
Native 1.6g.30% muscardine is made by the preparation method input mixing (with reference to embodiment 1) of water-dispersible granules in above raw material
Thiacloprid water dispersible granules.Muscardine pulvis used/gram containing beauveria bassiana spore 20,000,000,000, thiacloprid active compound content is 95%, real
When calculating is prepared with muscardine quality ratio in border, calculated with pure thiacloprid.The microbial insecticide composition of the preventing and treating red fire ant
Diluted 72 hours after spraying, medicine by 100-150 times and check drug effect.The microbial insecticide composition of the preventing and treating red fire ant is to flourishing
The prevention effect of ant is very good, has efficient, quick advantage to red fire ant.
Claims (3)
1. a kind of microbial insecticide composition for preventing and treating red fire ant, it is characterised in that including muscardine and thiacloprid, muscardine
The number of spore and the grams ratio of thiacloprid are：(8~1) × 20,000,000,000：2~9g.
2. the microbial insecticide composition of preventing and treating red fire ant according to claim 1, it is characterised in that beauveria bassiana spore
Number and the grams ratio of thiacloprid be：8 × 20,000,000,000：2g.
3. the microbial insecticide composition of preventing and treating red fire ant according to claim 1, it is characterised in that described preventing and treating
The microbial insecticide composition of red fire ant is water-dispersible granules, based on mass fraction 100%, including muscardine pulvis and thiophene
The total mass fraction of worm quinoline is 30%, and muscardine pulvis and thiacloprid mass ratio are 8~1：2~9, described muscardine pulvis contains
Have 20,000,000,000/g of beauveria bassiana spore, wetting agent pull open powder 8%, dispersant sodium lignin sulfonate 18%, disintegrant calcium chloride 5%,
Stabilizer disodium hydrogen phosphate 2%, adhesive Sodium Polyacrylate 5% and carrier diatomaceous earth 32%.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107624797B (en) | 2017-09-15 | 2020-08-04 | 广东省生物资源应用研究所 | Preparation method of biopesticide preparation for preventing and controlling red imported fire ants |
| CN107455389B (en) * | 2017-09-15 | 2020-04-14 | 广东省生物资源应用研究所 | Synergistic composition of biopesticide preparation for preventing and controlling red imported fire ants |
| CN107668074B (en) * | 2017-09-15 | 2020-12-01 | 广东省科学院动物研究所 | Biological prevention and control method for longhorn beetles |
| CN115152789A (en) * | 2022-07-21 | 2022-10-11 | 华南农业大学 | Application of activated clay in preventing and treating red imported fire ants |
Citations (3)
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|---|---|---|---|---|
| CN1792148A (en) * | 2005-12-30 | 2006-06-28 | 广州新天地化学实业有限公司 | Bait formulation for killing red ants |
| CN1909789A (en) * | 2004-01-09 | 2007-02-07 | Fmc有限公司 | Insecticidal compositions for control of general household pests |
| CN101069518A (en) * | 2007-04-30 | 2007-11-14 | 珠海经济特区瑞农植保技术有限公司 | Bait for killing solenopsis invicta Buren |
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2015
- 2015-03-09 CN CN201510103305.XA patent/CN104782663B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1909789A (en) * | 2004-01-09 | 2007-02-07 | Fmc有限公司 | Insecticidal compositions for control of general household pests |
| CN1792148A (en) * | 2005-12-30 | 2006-06-28 | 广州新天地化学实业有限公司 | Bait formulation for killing red ants |
| CN101069518A (en) * | 2007-04-30 | 2007-11-14 | 珠海经济特区瑞农植保技术有限公司 | Bait for killing solenopsis invicta Buren |
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| 入侵红火蚁预防控制及其防治研究;杨天赐等;《中国媒介生物学及控制杂志》;20060220;第17卷(第1期);第72-74页；第73页左栏第4段 * |
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Address after: 510260 No. 105 West Xingang Road, Guangzhou, Guangdong, Haizhuqu District Patentee after: Institute of zoology, Guangdong Academy of Sciences Address before: 510260 No. 105 West Xingang Road, Guangzhou, Guangdong, Haizhuqu District Patentee before: Guangdong Institute of Applied Biological Resources |