Content of the invention
The purpose of the present invention is to propose to a kind of have that synergistic function, use cost be low, preventive effect is good containing acequinocyl
High-efficient pesticide composition.
The present invention is to be achieved through the following technical solutions:
A kind of high-efficient pesticide composition containing acequinocyl, containing active components A and active component B, active components A and activity
For 1 60 ~ 80 1, described active components A is selected from acequinocyl to composition B weight ratio, and active component B is selected from avilamycin, first cyanogen chrysanthemum
One of ester, Biphenthrin are planted;Active components A with active component B preferred weight than for 1 40 ~ 60 1;More preferably go out demodicid mite
The weight of quinone and avilamycin than for 15 ~ 40 1, the weight of acequinocyl and Fenpropathrin than for 1 30 ~ 20 1, acequinocyl and connection
The weight of benzene chrysanthemum ester is than for 1 10 ~ 30 1;The weight ratio of most preferably acequinocyl and avilamycin for 11 ~ 15 1, acequinocyl with
Than for 1 15 ~ 10 1, the weight of acequinocyl and Biphenthrin is than for 15 ~ 15 1 for the weight of Fenpropathrin.
The described high-efficient pesticide composition containing acequinocyl, compositionss make wettable powder, water dispersible granules, suspension
Agent, suspension emulsion, aqueous emulsion, microemulsion, microcapsule suspending agent, microcapsule suspension-suspendinagent agent, can also make soluble liquid.
The described high-efficient pesticide composition containing acequinocyl is used for preventing and treating the application of crop pests, described crops bag
Include cereal crops, legume crop, fibre crops, sugar crop, melon crop, fruits crop, dry fruit crop, hobby work
Thing, root crop, oil crop, flower crop, medicinal crop, crop, green manure pasture crop;Described insect includes
Red spider, tetranychus viennensis, Tetranychus urticae, Panonychus citri, beginning tetranychid, Tetranychus cinnabarinus, Folium Camelliae sinensis demodicid mite, Shenmu-Yanan railway, Fructus Lycopersici esculenti wart demodicid mite, cut
Type tetranychid, rust mite, tetranychus telariuses, wart demodicid mite, diamondback moth, Pieris rapae, leaf miner, blister mite, bollworm, small heart-eating peach worm, tea are little
Greenery Cicadae, tea geometrid, weevil, trialeurodes vaporariorum, liriomyza bryoniae, pear sucker, rice leaf roller.
In the present composition, the content of active component depends on amount of application when being used alone, and also depends on a kind of chemical combination
The mixed ratio of thing and another kind of compound and potentiation degree, simultaneously also relevant with target pest.Generally in compositionss
The weight percentage of active component is the 1%~90% of gross weight, preferably 5%~80%.According to different preparation types, live
Property component content scope is different.Generally, liquid preparation contains by weight 1%~60% active substance, and preferably 5%
~50%;Solid preparation contains by weight 5%~80% active substance, and preferably 10%~80%.
At least contain a kind of surfactant in the composition pesticide of the present invention, be beneficial to during administration active component in water
Dispersion.Surface-active contents are the 2%~30% of total formulation weight, balance of solid or liquid diluent.
Surfactant selected by the composition pesticide of the present invention is known in those skilled in the art:Can be selected from
One or more of dispersant, wetting agent, thickening agent or defoamer.According to different dosage form, this area in preparation, can also be contained
Stabilizer well known to technical staff, antifreeze etc..
The composition pesticide of the present invention can be by user before use through dilution or directly use.Its preparation can be by ability
Processing method preparation well known to field technique personnel, will add after active component mixed with liquid flux or solid carrier
Surfactant such as one or more of dispersant, stabilizer, wetting agent, binding agent, defoamer etc..
Compositionss are made and are comprised following component and content during wettable powder:Active components A 0.1
% ~ 80%, active component B0.1% ~ 60%, dispersant 1% ~ 12%, wetting agent 1% ~ 8%, filler surplus.
Compositionss are made and are included following component and content during water dispersible granules:Active components A 0.5% ~ 80%, active component
B0.5% ~ 60%, dispersant 1% ~ 12%, wetting agent 1% ~ 8%, disintegrating agent 1% ~ 10%, binding agent 0 ~ 8%, filler surplus.
Compositionss are made and are included following component and content during suspending agent:Active components A 0.5% ~ 50%, active component B0.5%
~ 50%, dispersant 1% ~ 10%, wetting agent 1% ~ 10%, defoamer 0.01% ~ 2%, thickening agent 0 ~ 2%, antifreeze 0 ~ 8%, deionized water
Add to 100%.
Compositionss are made and are included following component and content during suspension emulsion:Active components A 0.5% ~ 50%, active component B0.5%
~ 50%, emulsifying agent 1% ~ 10%, dispersant 1% ~ 10%, solvent 1% ~ 20%, defoamer 0.01% ~ 2%, thickening agent 0 ~ 2%, antifreeze 0 ~
8%th, deionized water adds to 100%.
Compositionss are made and are included following component and content during microemulsion:Active components A 0.5% ~ 50%, active component B0.5% ~
50%th, emulsifying agent 3% ~ 25%, solvent 1% ~ 20%, antifreeze 0 ~ 8%, defoamer 0.01% ~ 2%, deionized water add to 100%.
Compositionss are made and are included following component and content during aqueous emulsion:Active components A 0.5% ~ 50%, active component B0.5%
~ 50%, solvent 1% ~ 20%, emulsifying agent 1% ~ 12%, antifreeze 0 ~ 8%, defoamer 0.01% ~ 2%, thickening agent 0 ~ 2%, deionized water add
To 100%.
Compositionss are made and are included following component and content during microcapsule suspending agent:Active components A 0.5% ~ 50%, active component
B0.5% ~ 50%, macromolecule cyst material 1% ~ 10%, dispersant 2% ~ 10%, solvent 1% ~ 10%, emulsifying agent 1% ~ 7%, pH adjusting agent
0.01% ~ 5%, defoamer 0.01% ~ 2%, deionized water add to 100%.
Compositionss are made and are included following component and content during microcapsule suspension-suspendinagent agent:Active components A 0.5% ~ 50%, activity
Composition B0.5% ~ 50%, macromolecule cyst material 1% ~ 12%, dispersant 1% ~ 12%, wetting agent 1% ~ 8%, solvent 1% ~ 15%, emulsifying agent
1% ~ 8%, defoamer 0.01% ~ 2%, thickening agent 0 ~ 2%, pH adjusting agent 0.01% ~ 5%, deionized water add to 100%.
The wettable powder the key technical indexes of the present invention:
The water dispersible granules the key technical indexes of the present invention:
The suspending agent the key technical indexes of the present invention:
The suspension emulsion the key technical indexes of the present invention:
The microemulsion the key technical indexes of the present invention:
The aqueous emulsion the key technical indexes of the present invention:
The microcapsule suspending agent the key technical indexes of the present invention:
The microcapsule suspension-suspendinagent agent the key technical indexes of the present invention:
It is an advantage of the current invention that:
(1)The present composition has good potentiation and lasting effect within the specific limits, and preventive effect is higher than single dose, thus subtracting
Few pesticide usage amount, while reducing peasant's drug cost, reduces residual quantity on crop for the pesticide, mitigates environmental pollution;
(2)Between kind, structure difference is larger, and the resistance such that it is able to delay single dose occurs and development;(3)Expand insecticidal spectrum, to farming
Various pests on thing have greater activity;(4)To person poultry safety, Environmental compatibility is good;And preparation adhesion strength strengthens, resistance to rain
Water washes away.
Specific embodiment
With reference to embodiment to further instruction of the present invention, the percentage ratio in embodiment is all weight percentage, but
The invention is not limited in this.
Application Example one
Example 1 ~ 8 wettable powder
By acequinocyl, active component B, dispersant, wetting agent, filler mixing, mix homogeneously in mixed cylinder, through air-flow powder
Broken machine remixes uniformly after pulverizing, you can make wettable powder product of the present invention.Specifically it is shown in Table 1.
The each component of table 1 example 1 ~ 8 and content
Example 9 ~ 16 water dispersible granules
Acequinocyl, active component B, dispersant, wetting agent, disintegrating agent, filler are obtained needs through comminution by gas stream together
Particle diameter, adds binding agent(Can plus can be not added with)Deng other auxiliary agents, obtain granulation materials.Item is quantitatively sent into fluidized bed granulation
In drying machine after granulation and drying, of the present invention water dispersible granules product is obtained.Specifically it is shown in Table 2.
The each component of table 2 example 9 ~ 16 and content
Example 17 ~ 24 suspending agent
By dispersant, wetting agent, defoamer, thickening agent(Can plus can be not added with), antifreeze(Can plus can be not added with)Through at a high speed
Shear-mixed uniformly, adds acequinocyl, active component B, and surplus deionized water is supplied, and in ball mill, ball milling 2 ~ 3 hours, make
Diameter of particle, all below 5 μm, is obtained suspending agent product of the present invention.Specifically it is shown in Table 3.
The each component of table 3 example 17 ~ 24 and content
Example 25 ~ 28 suspension emulsion
By dispersant, defoamer, thickening agent(Can plus can be not added with), antifreeze(Can plus can be not added with)Through high speed shear mixing
Uniformly, add acequinocyl, ball milling 2 ~ 3 hours in ball mill, make diameter of particle all below 5 μm, prepared acequinocyl suspends
Agent, then by active component B, solvent, emulsifying agent and various auxiliary agent with the direct emulsifying of high speed agitator in suspending agent, be obtained this
The described suspension emulsion product of invention.Specifically it is shown in Table 4.
The each component of table 4 example 25 ~ 28 and content
Example 29 ~ 36 aqueous emulsion
Acequinocyl, active component B, solvent, emulsifying agent are added together, make to be dissolved into homogeneous oil phase;By deionized water, resist
Freeze agent(Can plus can be not added with), thickening agent(Can plus can be not added with), defoamer mix, become homogeneous aqueous phase.Under high velocity agitation,
Aqueous phase is added oil phase, of the present invention aqueous emulsion product is obtained.Specifically it is shown in Table 5.
The each component of table 5 example 29 ~ 36 and content
Avilamycin, Fenpropathrin, Biphenthrin in table 1 to table 5 are exchanged, novel formulation can be obtained.
Example 37 ~ 39 microemulsion
Acequinocyl, active component B are dissolved in equipped with the homogenizer of solvent, by emulsifying agent, antifreeze(Can plus can not
Plus), defoamer be added in the homogenizer equipped with above-mentioned solution, surplus deionized water mixes and even after supplying strongly
Change, finally obtain the transparent microemulsion product of the present invention of clear appearance.Specifically it is shown in Table 6.
The each component of table 6 example 37 ~ 39 and content
Example 40 ~ 42 microcapsule suspending agent
By acequinocyl, active component B, macromolecule cyst material, solvent mixing, make to be dissolved into homogeneous oil phase, in shearing bar
Under part, oil phase is added to containing in emulsifying agent, pH adjusting agent, dispersant, the aqueous phase solution of defoamer, surplus deionized water
Supply, bi-material reacts in oil-water interfaces, form high score ascus wall, make the finely disseminated microcapsule of the present composition
Suspending agent product.Specifically it is shown in Table 7.
The each component of table 7 example 40 ~ 42 and content
Example 43 ~ 45 microcapsule suspension-suspendinagent agent
By active component B, macromolecule cyst material, solvent mixing, make to be dissolved into homogeneous oil phase, by oil phase in shearing condition
Under be added to containing in emulsifying agent, the aqueous phase solution of pH adjusting agent, make finely disseminated microcapsule suspending agent.By dispersant, wet
Profit agent, defoamer, thickening agent(Can plus can be not added with)Through high speed shear mix homogeneously, add acequinocyl, ball milling 2 in ball mill
~ 3 hours, make diameter of particle all below 5 μm, prepared suspending agent, then suspending agent is added to the water of micro-capsule suspension
In phase solution, deionized water supplies surplus, makes the present composition finely disseminated microcapsule suspension-suspendinagent agent product.Specifically
It is shown in Table 8.
The each component of table 8 example 43 ~ 45 and content
The embodiment of the present invention is the method being combined using Toxicity Determination and field test.First pass through indoor virulence to survey
Fixed, after clear and definite two kinds of medicaments are compounding by a certain percentage co-toxicity coefficient(CTC), CTC < 80 is antagonism, 80≤CTC≤120
For summation action, CTC > 120 is potentiation, on this basis, then carries out field test.
Test method:Respectively the mother solution of each mixed agent is diluted to five series concentration during test, is respectively placed in beaker
Standby.Using first soaking the method that leaf is followed by worm, by the blade of the same size not in contact with any medicament in the medicinal liquid having configured
Take out after soaking 5s, naturally dry, put in insect box, then connect for examination larva, raise under the conditions of 25 DEG C, often process 3
Secondary repetition, often repeats test worm number used and is 50, set blank simultaneously, check dead borer population in 72h, calculate mortality rate and correction
Mortality rate, tries to achieve virulence regression equation and calculates LC50Value.If comparison mortality rate is more than 10%, it is considered as invalid test.Calculate public
Formula is as follows:
Insect corrected mortality is converted into probit value(y), concentration for the treatment of(μg/ml)It is converted into logarithm value(x), with minimum
Square law draws virulence regression equation, and thus calculates the value of every kind of medicament.Calculate common poison system according to the abundant equation of Sun Yun
Number CTC.Computing formula is as follows(With acequinocyl as standard agent, its toxicity index is 100):
The theoretical toxicity index of M(TTI)TI × the P of=acequinocylAcequinocylTI × the P of+active component BEffective active composition B
In formula:M is the mixture of different ratio
PEffective active composition BFor effective active composition B ratio shared in the composition
PAcequinocylFor acequinocyl ratio shared in the composition
B is selected from one of avilamycin, Fenpropathrin, Biphenthrin and plants.
Application Example two:
For trying insect:Citrus red mite.
Test medicine is provided by Shaanxi Meibang Pesticide Co., Ltd..
EXPERIMENTAL DESIGN:Determine the effective of acequinocyl and the former medicine of AVM and the two different ratio mixture through pilot study
Lethasl concentration scope.
Toxicity test result
Table 9 acequinocyl and the abamectin compounded toxicity test analysis of results table to citrus red mite
As shown in Table 9, acequinocyl and the proportioning of abamectin compounded preventing and treating citrus red mite are when 1 60 ~ 80 1, malicious altogether
Coefficient is all higher than 120, illustrates that both all show potentiation, when acequinocyl and Avermectin at mixture in the range of 1 60 ~ 80 1
, when 15 ~ 40 1, potentiation is more prominent, and co-toxicity coefficient is all higher than 225 for the proportioning of element, goes out demodicid mite through applicant's test discovery
Quinone is 40 with the optimum ratio of avilamycin:1、35:1、30:1、25:1、20:1、15:1、14:1、13:1、12:1、11:1、10:
1、9:1、8:1、7:1、6:1、5:1、4:1、3:1、2:1、1:1、1:2、1:3、1:4、1:5, wherein when acequinocyl and avilamycin
Weight is more maximum than for co-toxicity coefficient when 81, and potentiation is the most obvious.
Application Example three:
For trying insect:European red mite.
Test medicine is provided by Shaanxi Meibang Pesticide Co., Ltd..
EXPERIMENTAL DESIGN:Determine the effective of acequinocyl and Fenpropathrin active compound and the two different ratio mixture through pilot study
Lethasl concentration scope.
Toxicity test result
Table 10 acequinocyl compounds the toxicity test analysis of results table to European red mite with Fenpropathrin
As shown in Table 10, acequinocyl and Fenpropathrin compound the proportioning of preventing and treating European red mite when 1 60 ~ 80 1, poison altogether
Coefficient is all higher than 120, illustrates that both all show potentiation at mixture in the range of 1 60 ~ 80 1, when acequinocyl and first cyanogen chrysanthemum
, when 1 30 ~ 20 1, potentiation is more prominent, and co-toxicity coefficient is all higher than 220 for the proportioning of ester, goes out demodicid mite through applicant's test discovery
Quinone is 20 with the optimum ratio of Fenpropathrin:1、15:1、10:1、9:1、8:1、7:1、6:1、5:1、4:1、3:1、2:1、1:1、2:
3、1:2、1:3、1:4、1:5、1:6、1:7、1:8、1:9、1:10、1:15、1:20、1:25、1:30, wherein when acequinocyl and first cyanogen
Chrysanthemum ester weight is more maximum than for co-toxicity coefficient when 23, and potentiation is the most obvious.
Application Example four:
For trying insect:Cotton red spider.
Test medicine is provided by Shaanxi Meibang Pesticide Co., Ltd..
EXPERIMENTAL DESIGN:Determine the effective of acequinocyl and Biphenthrin active compound and the two different ratio mixture through pilot study
Lethasl concentration scope.
Toxicity test result
Table 11 acequinocyl compounds the toxicity test analysis of results table to cotton red spider with Biphenthrin
As shown in Table 11, acequinocyl and Biphenthrin compound the proportioning of preventing and treating cotton red spider when 1 60 ~ 80 1, poison altogether
Coefficient is all higher than 120, illustrates that both all show potentiation at mixture in the range of 1 60 ~ 80 1, when acequinocyl and biphenyl chrysanthemum
, when 1 10 ~ 30 1, potentiation is more prominent, and co-toxicity coefficient is all higher than 225 for the proportioning of ester, goes out demodicid mite through applicant's test discovery
Quinone is 30 with the optimum ratio of Biphenthrin:1、25:1、20:1、15:1、10:1、9:1、8:1、7:1、6:1、5:1、4:1、3:1、
2:1、1:1、1:2、1:3、1:4、1:5、1:6、1:7、1:8、1:9、1:10, wherein when acequinocyl and Biphenthrin weight are than for 3
When 1, co-toxicity coefficient is maximum, and potentiation is the most obvious.
Find through test:Acequinocyl and active component B compound after to red spider, tetranychus viennensis, Tetranychus urticae, Panonychus citri,
Beginning tetranychid, Tetranychus cinnabarinus, Folium Camelliae sinensis demodicid mite, Shenmu-Yanan railway, Fructus Lycopersici esculenti wart demodicid mite, section type tetranychid, rust mite, tetranychus telariuses, wart demodicid mite, pickles
Moth, Pieris rapae, leaf miner, blister mite, bollworm, small heart-eating peach worm, tea lesser leafhopper, tea geometrid, weevil, trialeurodes vaporariorum, speckle are dived
Fly, pear sucker, the preventing and treating of rice leaf roller have obvious potentiation, and co-toxicity coefficient is all higher than 120.
Application Example five acequinocyl and active component B and its compounding preventing and treating citrus red mite test of pesticide effectiveness
This experimental establishment in Shaanxi Province's Hanzhong City, researched and developed by Shaanxi Meibang Pesticide Co., Ltd., provide, comparison by test medicine
Medicament 10% acequinocyl suspending agent(Autogamy), 1% abamectin emulsifiable concentrate(Commercial), 20% Fenpropathrin wettable powder(Commercial)、5%
Biphenthrin suspending agent(Commercial).
Investigate citrus red mite insect pest index before medicine, in insect pest early period of origination dispenser, 10 days after dispenser, adjust within 30 days, 45 days
Look into insect pest index and calculate preventive effect.Result of the test is as follows:
Table 12 acequinocyl and active component B and its compounding preventing and treating citrus red mite test of pesticide effectiveness
As can be seen from Table 12, acequinocyl can effectively prevent and treat citrus red mite after compounding with active component B, and prevention effect is equal
Better than the preventive effect of single dose, and preventive effect phase is long.In test scope of medication, target crop is had no adverse effects.
Application Example six acequinocyl and active component B and its compounding preventing and treating Fructus Mali pumilae Tetranychus urticae test of pesticide effectiveness
This experimental establishment in Xianyang, Shanxi province city, researched and developed by Shaanxi Meibang Pesticide Co., Ltd., provide, comparison by test medicine
Medicament 10% acequinocyl suspending agent(Autogamy), 1% abamectin emulsifiable concentrate(Commercial), 20% Fenpropathrin wettable powder(Commercial)、5%
Biphenthrin suspending agent(Commercial).
Fructus Mali pumilae Tetranychus urticae insect pest index is investigated, in insect pest early period of origination dispenser, 10 days after dispenser, 30 days, 45 days before medicine
Investigation insect pest index simultaneously calculates preventive effect.Result of the test is as follows:
Table 13 acequinocyl and active component B and its compounding preventing and treating Fructus Mali pumilae Tetranychus urticae test of pesticide effectiveness
As can be seen from Table 13, acequinocyl can effectively prevent and treat Fructus Mali pumilae Tetranychus urticae, prevention effect after compounding with active component B
It is superior to the preventive effect of single dose, and preventive effect phase is long.In test scope of medication, target crop is had no adverse effects.
Application Example seven acequinocyl and active component B and its compounding preventing and treating cotton red spider test of pesticide effectiveness
This experimental establishment is researched and developed, is carried by Shaanxi Meibang Pesticide Co., Ltd. in Shaanxi Province Weinan City Dali County, test medicine
For comparison medicament 10% acequinocyl suspending agent(Autogamy), 1% abamectin emulsifiable concentrate(Commercial), 20% Fenpropathrin wettable powder
(Commercial), 5% Biphenthrin suspending agent(Commercial).
Investigate cotton red spider insect pest index before medicine, in insect pest early period of origination dispenser, 10 days after dispenser, adjust within 20 days, 30 days
Look into insect pest index and calculate preventive effect.Result of the test is as follows:
Table 14 acequinocyl and active component B and its compounding preventing and treating cotton red spider test of pesticide effectiveness
As can be seen from Table 14, acequinocyl can effectively prevent and treat cotton red spider after compounding with active component B, and prevention effect is equal
Better than the preventive effect of single dose, and preventive effect phase is long.In test scope of medication, target crop is had no adverse effects.
Application Example eight acequinocyl and active component B and its compounding preventing and treating Fructus Lycopersici esculenti wart demodicid mite test of pesticide effectiveness
This experimental establishment in Xian District, Shanxi Province suburban area, researched and developed by Shaanxi Meibang Pesticide Co., Ltd., provide by test medicine,
Comparison medicament 10% acequinocyl suspending agent(Autogamy), 1% abamectin emulsifiable concentrate(Commercial), 20% Fenpropathrin wettable powder(City
Purchase), 5% Biphenthrin suspending agent(Commercial).
Before medicine, investigation Fructus Lycopersici esculenti wart acarid does harm to index, in insect pest early period of origination dispenser, 10 days after dispenser, investigates within 20 days, 30 days
Insect pest index simultaneously calculates preventive effect.Result of the test is as follows:
Table 15 acequinocyl and active component B and its compounding preventing and treating Fructus Lycopersici esculenti wart demodicid mite test of pesticide effectiveness
As can be seen from Table 15, acequinocyl can effectively prevent and treat Fructus Lycopersici esculenti wart demodicid mite after compounding with active component B, and prevention effect is all excellent
In the preventive effect of single dose, and preventive effect phase is long.In test scope of medication, target crop is had no adverse effects.
Application Example nine acequinocyl and active component B and its compounding preventing and treating brassicaceous vegetable diamondback moth test of pesticide effectiveness
This experimental establishment in Xian District, Shanxi Province suburban area, researched and developed by Shaanxi Meibang Pesticide Co., Ltd., provide by test medicine,
Comparison medicament 10% acequinocyl suspending agent(Autogamy), 1% abamectin emulsifiable concentrate(Commercial), 20% Fenpropathrin wettable powder(City
Purchase), 5% Biphenthrin suspending agent(Commercial).
Brassicaceous vegetable diamondback moth insect pest index is investigated, in insect pest early period of origination dispenser, 10 days after dispenser, 20 before medicine
My god, 30 days investigation insect pest indexes calculate preventive effect.Result of the test is as follows:
Table 16 acequinocyl and active component B and its compounding preventing and treating brassicaceous vegetable diamondback moth test of pesticide effectiveness
As can be seen from Table 16, acequinocyl can effectively prevent and treat brassicaceous vegetable diamondback moth after compounding with active component B, prevents
Control the preventive effect that effect is superior to single dose, and preventive effect phase is long.In test scope of medication, target crop is had no adverse effects.
Test through different places throughout the country draws afterwards, acequinocyl and avilamycin, Fenpropathrin, Biphenthrin
To the red spider on crops, tetranychus viennensis, Tetranychus urticae, Panonychus citri, beginning tetranychid, Tetranychus cinnabarinus, Folium Camelliae sinensis demodicid mite, Shen Ze after compounding
Family name tetranychid, Fructus Lycopersici esculenti wart demodicid mite, section type tetranychid, rust mite, tetranychus telariuses, wart demodicid mite, diamondback moth, Pieris rapae, leaf miner, blister mite, cotton boll
The common worm such as worm, small heart-eating peach worm, tea lesser leafhopper, tea geometrid, weevil, trialeurodes vaporariorum, liriomyza bryoniae, pear sucker, rice leaf roller
, all more than 95%, better than single dose preventive effect, potentiation is obvious for the preventive effect of evil.