CN104774812A - Processing method for extraction of superoxide dismutase from corn germ - Google Patents
Processing method for extraction of superoxide dismutase from corn germ Download PDFInfo
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- C12Y115/00—Oxidoreductases acting on superoxide as acceptor (1.15)
- C12Y115/01—Oxidoreductases acting on superoxide as acceptor (1.15) with NAD or NADP as acceptor (1.15.1)
- C12Y115/01001—Superoxide dismutase (1.15.1.1)
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Abstract
The invention discloses a processing method for extraction of superoxide dismutase from corn germ, the corn germ is used as a raw material, by germ extraction, raw material production cost and manpower and material resources of subsequent process manpower and material resources can be greatly reduced, the raw material is pre processed by soaking in anhydrous ethanol, the membrane loss can be reduced; germ slurry is slowly frozen and then clarified, after solid-liquid separation, polysaccharides can be removed; and no acid, alkali, inorganic salt and reagents harmful to the body are added, the anhydrous ethanol used in oil and impurity removing and precipitation can be removed in the drying process because of easy volatility of the anhydrous ethanol, the anhydrous ethanol also can be effectively recycled, production cost is reduced, meanwhile the quality of superoxide dismutase dry powder can be ensured.
Description
Technical field
The present invention relates to superoxide-dismutase extraction process, particularly relate to the working method extracting superoxide-dismutase in a kind of maize germ.
Background technology
Superoxide-dismutase SOD is a kind of metalloenzyme (cupric, zinc, iron and manganese) be extensively formed in the bodies of aminal and plant, and being topmost antioxidant in human body, is the jinx of oxyradical and single-minded scavenging agent.Exogenous supplementary SOD, can improve the content of SOD in blood, decomposes in time and eliminates superfluous free radical, thus protecting organism normal cell tissue, avoiding the generation of disease, people is kept fit, full of physical strength.
The production of current superoxide-dismutase is mainly derived from animal blood and liver, and extracts SOD and easily produce viral cross infection from animal blood or tissue.Therefore the extraction exploitation of plant SOD to medical treatment, the new drug that keeps healthy, overcome the virulence factor that animal source goods may exist and have very important significance.
Countries in the world are to the effect identification of " superoxide-dismutase ":
The U.S. Register patent No. is (CN1152337A): for prevention and therapy brain local hemorrhage, ulcer, diminish inflammation, heart disorder, edema, poisoning, the disease such as rheumatism, similar rheumatism, rheumatic arthritis, radiation injury, drug intoxication.
France's registered patent number is (CN1167441A): for prevention and therapy specificity brain injury, non-specific brain injury and the brain function cognitive disorders that causes.
The European registration patent No. is (499621): the brain injury brought for prevention and therapy cancer and postoperative Radiotherapy chemotherapy, and anticancer is formed or spreads.
Japanese Registered Patent number is (4327541,4312533): be used for the treatment of the anti-immune response after organ transplantation and have special effect.Be used for the treatment of brain local hemorrhage and cause nerve necrosis, prevent the medicine of hemiplegia.
Taibei hospital " superoxide-dismutase (SOD) " treatment burn, scald, comparative experiments succeed, and report: smear 3 times that affected part healing is routine treatment with " superoxide-dismutase (SOD) ", and scar obviously alleviates.
The Anderson of houston, u.s.a University of Texas, doctor Chinese Huang Peng and his colleagues of Cancer center find that superoxide-dismutase (SOD) optionally can kill leukemia cell, and do not damage normal tissue cell.
Superoxide-dismutase (SOD) is free-radical scavengers, it is extensively present in the various tissues of organism, energy scavenging free radicals O2(ultra-oxygen anion free radical), and O2 has cytotoxicity, can make lipid peroxidation, damaging cells film, causes inflammation, tumour and autoimmune disorder, and may body aging be impelled.Superoxide-dismutase (SOD) normal value is enzyme rate method (37 DEG C) (1): serum: 242 ~ 620U/L; Red corpuscle: 5375 ~ 7975 μ g/gHb are the search of pyrogallol method health (2): cardiac muscle: 300.3 ~ 406.3U/g; Red corpuscle health search: 1567 ~ 2241U/g.(3) dianisidine method: cardiac muscle: 1745 ~ 2231U/g; Red corpuscle: 2906 ~ 4654U/g.
1, cardiovascular and cerebrovascular diseases is suppressed:
In the aging of body and body, the generation of oxyradical is closely related with accumulation, and SOD can remove harmful oxygen too much in human body freely, is the useful functional component to health.There is the health-care effect of adjusting blood lipid, can prevention of arterial atherosis, the cardiovascular and cerebrovascular diseases that causes of prevention hyperlipidemia.Reduce the content of lipid peroxide.
2, anti-ageing:
The growth at age and some extrinsic factor body and skin histology free radical can be caused to produce exceed the normal scavenging free radicals of body ability, thus skin histology is damaged, causes aging.Because SOD can scavenging free radicals, thus can delay senility.Why people can be old and feeble, aging sign goes out to see little by little, and as pigementation, flag, be because produce oxygenizement in body, what is called " oxygenizement " is just similar to gets rusty, being supplemented with of antioxidant helps fall protoxydic speed, and slow down old and feeble step.
3, autoimmune disorder is prevented and treated:
SOD has certain curative effect to all kinds of autoimmune disorder.As lupus erythematosus, scleroderma, dermatomyositis etc.Should not form front use in acute phase pathology for rheumatoid arthritis patients, curative effect is better.
4, pulmonary emphysema:
Emphysema patient also can use SOD, but should use when the pathology initial stage, lung elastance fiber not yet suffered damage, and curative effect is better.
5, Radiation sickness and radio-protective:
These product can be used to treat the diseases such as urocystitis, dermatomyositis, lupus erythematosus and the oligoleukocythemia caused because of radiotherapy, and to the personnel being likely subject to ionizing rays, also injectable SOD is as preventive measures.
6, senile cataract:
Should namely start often to take antioxidant before entering the senium to this kind of disease, often inject SOD in other words.Once formation cataract, then should extract, because now use SOD invalid.
7, anti-oxidant:
Medical report is pointed out, the paracme of resistance of oxidation is advanced to about 40 years old, depends vegetables and fruits alone and has been not enough to eliminate the endectoplastic oxidative pressure of human body.
8, Chronic disease prevention:
Free radical is that scientist has found to cause various chronic disease and aging arch-criminal, therefore say that it is " sources of ten thousand diseases ", the formidable enemy of HUMAN HEALTH, the injury of radical pair health is accumulated over a long period, especially the disease of diabetes and cardiovascular aspect, Lin Tian send doctor to say: " look after your cardiovascular, just can live 90 years old." form the good custom the more absorbing polyphenoils, ensure to allow you away from the threat of chronic disease.
9, antifatigue:
Too much free radical is remaining in vivo, just the same in vivo just as toxin accumulation, can allow people: easily fatigue, be weary of, absent minded, usually have dizzy spells, yawn.SOD to go up for one's examination produced fatigue to stay up late overtime work, student of working clan, improving spirit and achieving noticeable achievement in focusing one's attention on, contributes to the lifting of job performance, and the progress of total marks of the examination.
10, side effect is eliminated:
The resistance of oxidation accepted in the cancer patient of chemotherapy can reduce widely, just in case low to certain degree, free radical will damage cell, mucous membrane, the vital organs of the human body, brain, nervus centralis etc. so cancer patients should supplement antioxidant in time to maintain physical stamina.Japanese health ministry and american cancer center (NCI) all advise using antioxidant to carry out preventing cancer or treat because oxyradical destroys pathology caused by cell, reduce caused by cancer therapy drug as the side effect such as vomiting, poor appetite, hair loss.
11, secondary injury is avoided:
Operation can cause a large amount of free radical, therefore the oral antioxidant of suggestion perioperatively regains one's strength rapidly, and accelerated in wounds is restored.
12, the crisis of women is dissolved:
The oxidation crisis of women has three:
A. skin spots wrinkle: because oxyradical cannot effectively be eliminated, destroy collagen protein, spandex albumen, make skin moisture-keeping and maintain elastic afunction, wrinkle grows wild, and accelerates melanic precipitation;
B. have some setbacks in poor circulation, menstrual period, the gloomy tarnish of black eye, the colour of skin;
C. climacterium obstacle: because estrogenic shortage, resistance of oxidation in body reduce, often have following symptom to occur: paroxysmal towards heat, have a sleepless night, night sweats, have a headache, emotional lability, have no peace of mind.
13, blood fat, cholesterol, blood pressure is effectively reduced;
14, radioprotective; 15, hepatic and renal function is strengthened; 16, obvious restitution is had to diabetes.
Based on described above, superoxide-dismutase is powerful, and demand is widely used, and current known extractive technique can not satisfy the demands.
Summary of the invention
For effectively solving the problems of the technologies described above, extracting the working method of superoxide-dismutase in maize germ provided by the invention, can meet technical need, concrete technical scheme is as described below:
Extract the working method of superoxide-dismutase in maize germ, the method comprises the following steps:
Step (1): choose maize germ and pulverize generation corn plumule powder, described corn plumule powder being inserted after soaking in dehydrated alcohol and drain;
Step (2): by the corn plumule powder separate solid waste residue after solid-liquid separation process after draining, gathers plumule stoste;
Step (3): freeze rear clarification by slow for described plumule stoste, gather the thick enzyme of liquid superoxide-dismutase;
Step (4): by the thick enzyme of described liquid superoxide-dismutase by generating superoxide-dismutase concentrated solution after ceramic membrane filter classified filtering;
Step (5): gradation adds dehydrated alcohol by volume in described superoxide-dismutase concentrated solution, by obtaining throw out after separating for several times, this throw out is the thick product of superoxide-dismutase;
Step (6): by water-soluble for thick for described superoxide-dismutase product rear solid-liquid separation, remove water-fast impurity, generate liquid superoxide-dismutase, obtains superoxide-dismutase dry powder by after described liquid superoxide-dismutase lyophilize.
One of preferably, the method comprises the following steps:
In described step (1): soaked in organic reagent by described corn plumule powder, after removing a large amount of oil substances in germ meal, in the maize germ pulverized, add dehydrated alcohol, soak about 30min and constantly stir evenly, then draining.
One of preferably, the method comprises the following steps:
In described step (2): carry out solid-liquid separation process again after need first being soaked by the corn plumule powder after draining.
One of preferably, the method comprises the following steps:
In described step (3): described plumule magma is slow freezes process time and control at 12 hours, more the more will be separated out by glucide, and effectively remove impurity by low temperature.
One of preferably, the method comprises the following steps:
In described step (4): in the process of ceramic membrane filter classified filtering, first by thick for liquid superoxide-dismutase enzyme through ceramic membrane filter, remove the visible suspended substance of molecular weight more than 250,000 and macromole impurity, obtain clear liquid; By described clear liquid again through twice filtering membrane, remove the impurity of molecular weight > 100,000 and molecular weight < 10,000 successively, obtain superoxide-dismutase concentrated solution.
One of preferably, the method comprises the following steps:
In described step (5): adopt dehydrated alcohol to carry out fractionation precipitation, remove impurity protein, the dehydrated alcohol that volume ratio is 30% is added in superoxide-dismutase concentrated solution, be separated and remove throw out, add in gained solution for continuous the dehydrated alcohol that volume ratio is 3:1, realize throw out afterwards and be separated.
Beneficial effect of the present invention:
1. reduce the cost, economize on resources.
In method provided by the invention, select maize germ to be extract raw material, superoxide-dismutase is mainly distributed in the plumule of corn grain, and plumule only accounts for 6% ~ 7% of whole corn grain.From output, the superoxide-dismutase dry powder weight of the maize germ gained of homogenous quantities is 8 ~ 10 times of the extraction gained from corn, and equally, obtain the superoxide-dismutase dry powder of equal in quality, the treatment capacity of corn is 8 ~ 10 times of plumule; Therefore from cost, plumule extracts can reduce the raw materials cost of production and the manpower and materials of subsequent technique greatly.
2. reduce film loss.
In the inventive method, the method for soaked in absolute ethyl alcohol is adopted to anticipate raw material.Although superoxide-dismutase is mainly distributed in the plumule of corn grain, also contain the fat of about 50% in plumule, the lipid content in solution is high, makes solution be milkiness state, causes very large puzzlement to membrane concentration simultaneously.Superoxide-dismutase belongs to water-soluble protein, is insoluble to organic reagent.According to similar dissolve mutually theory, fat can be dissolved in organic reagent, by soaked in absolute ethyl alcohol thus can reach remove fat effect, reduce the loss of film.
3. Polysaccharide removing.
In the inventive method, plumule slurries are carried out slow freezing and do clarifying treatment again.Slow freezing can make to produce thicker ice crystal in vegetable cell, and ice crystal is thicker, and cell tissue is easily injured, is even poked by sharp keen ice crystal, thus accelerates the precipitation of polysaccharide, can reach the object of Polysaccharide removing after solid-liquid separation.
4. quality guarantee.
The inventive method does not add any acid, alkali, inorganic salt and insalubrious reagent, and the dehydrated alcohol of degrease and removal of impurities, precipitation can remove in drying process because of its easy volatile.Dehydrated alcohol can also carry out efficient recovery, also ensure that the quality of superoxide-dismutase dry powder while reducing production cost.
Below in conjunction with accompanying drawing, the present invention is described in detail.
Accompanying drawing explanation
Fig. 1 is step (1) in the method for the invention-(4) schema;
Fig. 2 is step (4) in the method for the invention-(6) schema.
Embodiment
Embodiment 1:
As shown in Figures 1 and 2, the working method extracting superoxide-dismutase in this embodiment in maize germ comprises the following steps:
Step (1): choose maize germ and pulverize generation corn plumule powder, described corn plumule powder being inserted after soaking in dehydrated alcohol and drain; Concrete soaks described corn plumule powder in organic reagent, after removing a large amount of oil substances in germ meal, in the maize germ pulverized, adds dehydrated alcohol, soaks about 30min and constantly stir evenly, then draining.
Step (2): carry out solid-liquid separation process again, separate solid waste residue after solid-liquid separation process after need first being soaked by the corn plumule powder after draining, gather plumule stoste;
Step (3): freeze rear clarification by slow for described plumule stoste, gather the thick enzyme of liquid superoxide-dismutase; Described plumule magma is slow freezes process time and controls at 12 hours, more the more will be separated out by glucide, and effectively remove impurity by low temperature.
Step (4): by the thick enzyme of described liquid superoxide-dismutase by generating superoxide-dismutase concentrated solution after ceramic membrane filter classified filtering; In the process of ceramic membrane filter classified filtering, first by thick for liquid superoxide-dismutase enzyme through ceramic membrane filter, remove the visible suspended substance of molecular weight more than 250,000 and macromole impurity, obtain clear liquid; By described clear liquid again through twice filtering membrane, remove the impurity of molecular weight > 100,000 and molecular weight < 10,000 successively, obtain superoxide-dismutase concentrated solution.
Step (5): gradation adds dehydrated alcohol by volume in described superoxide-dismutase concentrated solution, by obtaining throw out after separating for several times, this throw out is the thick product of superoxide-dismutase; Dehydrated alcohol is adopted to carry out fractionation precipitation, remove impurity protein, in superoxide-dismutase concentrated solution, add the dehydrated alcohol that volume ratio is 30%, be separated and remove throw out, add in gained solution for continuous the dehydrated alcohol that volume ratio is 3:1, realize throw out afterwards and be separated.
Step (6): by water-soluble for thick for described superoxide-dismutase product rear solid-liquid separation, remove water-fast impurity, generate liquid superoxide-dismutase, obtains superoxide-dismutase dry powder by after described liquid superoxide-dismutase lyophilize.
Embodiment 2:
Extract the working method of superoxide-dismutase in this embodiment in maize germ, step is as follows:
The first step: get 50Kg maize germ and pulverize, add soaked in absolute ethyl alcohol, and constantly stir evenly, takes out after soaking 30min and drains.In the corn plumule powder processed, add 150L water, obtain the plumule stoste containing 120L superoxide-dismutase by flat whizzer.
Second step: freeze by tubular-bowl centrifuge solid-liquid separation again after 12h by slow in refrigerator for above-mentioned plumule stoste, the superoxide-dismutase crude enzyme liquid comparatively clarified.
3rd step: the superoxide-dismutase crude enzyme liquid upper step obtained is through a ceramic membrane filter and two times of ultrafiltration membrane filtration, ceramic membrane filter can remove the visible suspended substance of molecular weight more than 250,000 and macromole impurity, solution is more clarified, clear liquid is again through two times of ultrafiltration film, remove the impurity of molecular weight > 100,000 and molecular weight < 10,000 successively, obtain 8L superoxide-dismutase concentrated solution.
4th step: add 2.4L dehydrated alcohol in 8L superoxide-dismutase concentrated solution, centrifugal segregation throw out; In 9.2L superoxide-dismutase solution, add dehydrated alcohol 27.6L again, be isolated to throw out, be the thick product of superoxide-dismutase.
5th step: by again water-soluble for thick for the superoxide-dismutase of above-mentioned gained product, remove water-fast foreign protein after solid-liquid separation, obtain superoxide-dismutase enzyme liquid, enzyme liquid obtains superoxide-dismutase dry powder through lyophilize.
Embodiment 3:
Extract the working method of superoxide-dismutase in this embodiment in maize germ, step is as follows:
The first step: get 200Kg maize germ and pulverize, add soaked in absolute ethyl alcohol, and constantly stir evenly, takes out after soaking 30min and drains.In the corn plumule powder processed, add 600L water, obtain plumule stoste 500 L containing superoxide-dismutase by flat whizzer.
Second step: freeze by tubular-bowl centrifuge solid-liquid separation again after 12h by slow in refrigerator for above-mentioned plumule stoste, the superoxide-dismutase crude enzyme liquid comparatively clarified.
3rd step: the superoxide-dismutase crude enzyme liquid upper step obtained is through a ceramic membrane filter and two times of ultrafiltration membrane filtration, ceramic membrane filter can remove the visible suspended substance of molecular weight more than 250,000 and macromole impurity, solution is more clarified, clear liquid is again through two times of ultrafiltration film, remove the impurity of molecular weight > 100,000 and molecular weight < 10,000 successively, obtain superoxide-dismutase concentrated solution 25L.
4th step: add 7.5L dehydrated alcohol in superoxide-dismutase concentrated solution, centrifugal segregation throw out; Superoxide-dismutase solution adds 90L dehydrated alcohol again, is isolated to throw out, is the thick product of superoxide-dismutase.
5th step: by again water-soluble for thick for the superoxide-dismutase of above-mentioned gained product, remove water-fast foreign protein after solid-liquid separation, obtain superoxide-dismutase enzyme liquid, enzyme liquid obtains superoxide-dismutase dry powder through lyophilize.
The present invention is relative to the difference of conventional machining process:
1., in method provided by the invention, select maize germ to be extract raw material, plumule extracts can reduce the raw materials cost of production and the manpower and materials of subsequent technique greatly.
2., in the inventive method, adopt the method for soaked in absolute ethyl alcohol to anticipate raw material, reduce the loss of film.
3., in the inventive method, plumule slurries are carried out slow freezing and do clarifying treatment again, the object of Polysaccharide removing after solid-liquid separation, can be reached.
4. the inventive method does not add any acid, alkali, inorganic salt and insalubrious reagent, and the dehydrated alcohol of degrease and removal of impurities, precipitation can remove in drying process because of its easy volatile.Dehydrated alcohol can also carry out efficient recovery, also ensure that the quality of superoxide-dismutase dry powder while reducing production cost.
The invention is not restricted to above-mentioned enforcement, the numerous embodiments that all employing same principle and method thereof realize constructed effect all duplicates with this patent.
Claims (6)
1. extract the working method of superoxide-dismutase in maize germ, it is characterized in that, the method comprises the following steps:
Step (1): choose maize germ and pulverize generation corn plumule powder, described corn plumule powder being inserted after soaking in dehydrated alcohol and drain;
Step (2): by the corn plumule powder separate solid waste residue after solid-liquid separation process after draining, gathers plumule stoste;
Step (3): freeze rear clarification by slow for described plumule stoste, gather the thick enzyme of liquid superoxide-dismutase;
Step (4): by the thick enzyme of described liquid superoxide-dismutase by generating superoxide-dismutase concentrated solution after ceramic membrane filter classified filtering;
Step (5): gradation adds dehydrated alcohol by volume in described superoxide-dismutase concentrated solution, by obtaining throw out after separating for several times, this throw out is the thick product of superoxide-dismutase;
Step (6): by water-soluble for thick for described superoxide-dismutase product rear solid-liquid separation, remove water-fast impurity, generate liquid superoxide-dismutase, obtains superoxide-dismutase dry powder by after described liquid superoxide-dismutase lyophilize.
2. extract the working method of superoxide-dismutase in maize germ, it is characterized in that, the method comprises the following steps:
In described step (1): soaked in organic reagent by described corn plumule powder, after removing a large amount of oil substances in germ meal, in the maize germ pulverized, add dehydrated alcohol, soak about 30min and constantly stir evenly, then draining.
3. extract the working method of superoxide-dismutase in maize germ, it is characterized in that, the method comprises the following steps:
In described step (2): carry out solid-liquid separation process again after need first being soaked by the corn plumule powder after draining.
4. extract the working method of superoxide-dismutase in maize germ, it is characterized in that, the method comprises the following steps:
In described step (3): described plumule magma is slow freezes process time and control at 12 hours, more the more will be separated out by glucide, and effectively remove impurity by low temperature.
5. extract the working method of superoxide-dismutase in maize germ, it is characterized in that, the method comprises the following steps:
In described step (4): in the process of ceramic membrane filter classified filtering, first by thick for liquid superoxide-dismutase enzyme through ceramic membrane filter, remove the visible suspended substance of molecular weight more than 250,000 and macromole impurity, obtain clear liquid; By described clear liquid again through twice filtering membrane, remove the impurity of molecular weight > 100,000 and molecular weight < 10,000 successively, obtain superoxide-dismutase concentrated solution.
6. extract the working method of superoxide-dismutase in maize germ, it is characterized in that, the method comprises the following steps:
In described step (5): adopt dehydrated alcohol to carry out fractionation precipitation, remove impurity protein, the dehydrated alcohol that volume ratio is 30% is added in superoxide-dismutase concentrated solution, be separated and remove throw out, add in gained solution for continuous the dehydrated alcohol that volume ratio is 3:1, realize throw out afterwards and be separated.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106858592A (en) * | 2017-03-07 | 2017-06-20 | 青海极域生物资源开发有限公司 | Lycium ruthenicum pure powder tablet preparation method |
| CN111041007A (en) * | 2019-12-04 | 2020-04-21 | 上海润日生物科技发展有限公司 | Preparation method of superoxide dismutase |
| CN112646788A (en) * | 2020-12-28 | 2021-04-13 | 刘越伟 | Processing method for extracting superoxide dismutase from corn germ |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1215169A (en) * | 1997-06-30 | 1999-04-28 | 利顿系统有限公司 | Offset optical fibre rotary sensor coil |
| CN1428422A (en) * | 2001-12-24 | 2003-07-09 | 徐文中 | Large-scale production process of superoxide dismutase |
| CN1215169C (en) * | 2003-06-20 | 2005-08-17 | 王建英 | Production method of corn superoxide dismutase multienzyme |
| WO2007000619A1 (en) * | 2005-06-28 | 2007-01-04 | Life Science Investments Ltd | Use of a mixture of superoxide dismutase and catalase for treating inflammatory skin lesions |
| CN103191070A (en) * | 2013-03-29 | 2013-07-10 | 湖南大学 | Oral tablet of superoxide dismutase and preparation method thereof |
-
2014
- 2014-10-09 CN CN201410527113.7A patent/CN104774812A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1215169A (en) * | 1997-06-30 | 1999-04-28 | 利顿系统有限公司 | Offset optical fibre rotary sensor coil |
| CN1428422A (en) * | 2001-12-24 | 2003-07-09 | 徐文中 | Large-scale production process of superoxide dismutase |
| CN1215169C (en) * | 2003-06-20 | 2005-08-17 | 王建英 | Production method of corn superoxide dismutase multienzyme |
| WO2007000619A1 (en) * | 2005-06-28 | 2007-01-04 | Life Science Investments Ltd | Use of a mixture of superoxide dismutase and catalase for treating inflammatory skin lesions |
| CN103191070A (en) * | 2013-03-29 | 2013-07-10 | 湖南大学 | Oral tablet of superoxide dismutase and preparation method thereof |
Non-Patent Citations (2)
| Title |
|---|
| 廖传华等: "《超临界CO2流体萃取技术——工艺开发及其应用》", 31 July 2004 * |
| 王海灵: "玉米超氧化物歧化酶（SOD）提取及纯化技术的研究", 《中国优秀硕士学位论文全文数据库 工程科技I辑》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106858592A (en) * | 2017-03-07 | 2017-06-20 | 青海极域生物资源开发有限公司 | Lycium ruthenicum pure powder tablet preparation method |
| CN111041007A (en) * | 2019-12-04 | 2020-04-21 | 上海润日生物科技发展有限公司 | Preparation method of superoxide dismutase |
| CN112646788A (en) * | 2020-12-28 | 2021-04-13 | 刘越伟 | Processing method for extracting superoxide dismutase from corn germ |
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Application publication date: 20150715 |