CN103191070A - Oral tablet of superoxide dismutase and preparation method thereof - Google Patents
Oral tablet of superoxide dismutase and preparation method thereof Download PDFInfo
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- CN103191070A CN103191070A CN2013101062778A CN201310106277A CN103191070A CN 103191070 A CN103191070 A CN 103191070A CN 2013101062778 A CN2013101062778 A CN 2013101062778A CN 201310106277 A CN201310106277 A CN 201310106277A CN 103191070 A CN103191070 A CN 103191070A
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Abstract
The invention provides an oral tablet of superoxide dismutase and a preparation method thereof. The oral tablet comprises superoxide dismutase and a feed additive, wherein each g of oral tablet contains 14000-45000U of active superoxide dismutase; and proper superoxide dismutase dry powder of which the activity is 100000-500000 U/g is adopted in preparation, and pressed and molded after being mixed with the food additive. The SOD (superoxide dismutase) is slowly dissolved by spittle to gradually release the activity when the oral tablet is taken. Thus, the phenomenon that the SOD is inactivated under a gastric acid environment after entering into a stomach is avoided. The SOD dry powder in the oral tablet is extracted from corn; the cost of the SOD dry powder is greatly reduced when the activity is reserved to the maximal extent.
Description
Technical field
The present invention relates to a kind of oral biological preparation and preparation method, particularly a kind of superoxide dismutase oral buccal tablet and preparation method.
Background technology
Superoxide dismutase (English be called for short " SOD ") is the title with human body waste " street cleaner ", " anti-ageing king ", " beauty treatment favourite son " of generally acknowledging in the world, is antagonism " sources of all kinds of diseases and ailments " the strongest material of reactive oxygen free radical.Replenish needed by human body SOD by external source, can improve body immunity, slow down aging; Effective blood fat reducing, cholesterol, blood pressure; Resisting fatigue, radioprotective; Suppress cardiovascular and cerebrovascular disease etc.
Nowadays occur SOD health product very salable in a large number on the market, comprised SOD capsule, SOD oral liquid, SOD liquid preparation etc.We know that SOD is a kind of to the very sensitive metalloenzyme of pH value according to the characteristic of SOD, when SOD is taken by people with tablet or liquid reagent, can directly enter the stomach of human body, and the gastric acid of human body can destroy the three dimensional structure of SOD, and it is decomposed into peptide chain and aminoacid, thoroughly destroy the activity of SOD, so be difficult to guarantee to bring into play the SOD effect.For fear of this problem, SOD enteric coatel tablets and enteric coated capsule have appearred again in the world, the decomposition that these enteric coatel tablets or capsule can be avoided gastric acid directly reaches disintegrate behind the small intestinal, absorb by the small intestinal inwall, the blood of human body of entering is brought into play the effect of SOD, but in small intestinal under the complex environment, whether SOD can fully absorb and bring into play its effect and be difficult to determine.
Summary of the invention
The present invention aims to provide a kind of can fully the absorption and the tangible oral superoxide dismutase buccal tablet of effect by oral cavity and esophageal mucosa membrane injury, and a kind of lower-cost method for preparing this buccal tablet is provided.The present invention realizes by following scheme:
A kind of superoxide dismutase oral buccal tablet comprises superoxide dismutase and food additive, contains 1.4～4.5 ten thousand U active superoxide dismutase in the oral buccal tablet of every gram.
For strengthening mouthfeel, the food additive total amount accounts for 80%～90% of buccal tablet gross mass.
These food additive are the conventional additives of general buccal tablet for oral use, comprise: lubricated, releasing agent, (as magnesium stearate, sodium stearate etc.), the additive (as silicon dioxide) of anti-caking, the moisture absorption, disintegrating agent (as microcrystalline cellulose), binding agent (as hydroxypropyl fiber, hydroxypropyl amide etc.) and sweeting agent (as xylitol, mannitol etc.).The purity of all additives is food stage, and namely main constituent content is more than 99%.The addition of above-mentioned each food additive gets final product by the amount of common preparation oral buccal tablet.
The preparation method of above-mentioned superoxide dismutase oral buccal tablet, getting an amount of activity is the superoxide dismutase dry powder of 10U/g～500,000 U/g, after food additive is mixed, compression molding; The superoxide dismutase dry powder quality that adds during preparation is to account for 8%～15% of gross mass.
Above-mentioned superoxide dismutase dry powder adopts following steps to make:
The first step: Semen Maydis is carried out pretreatment obtain the Semen Maydis serosity;
Second step: in the Semen Maydis serosity, add water the dissolubility mantoquita, keep pH value 7～9, afterwards through solid-liquid separation, obtain superoxide dismutase stock solution;
The 3rd step: with the superoxide dismutase stock solution membrane filtration more than two-stage again of the second step gained, remove molecular weight successively〉120,000 and the impurity of molecular weight＜10,000, obtain the superoxide dismutase concentrated solution;
The 4th step: in the superoxide dismutase concentrated solution, add and to obtain precipitate with the Organic substance liquid that water dissolves each other through separating, the above-mentioned precipitate of lyophilization.
Be further to improve the purity of superoxide dismutase, can above-mentioned the 4th step gained dry powder water-soluble again after, adding can obtain precipitate with the Organic substance liquid that water dissolves each other through separating, the above-mentioned precipitate of lyophilization again.
The Organic substance liquid that can dissolve each other with water is preferred acetone, dehydrated alcohol or methanol on commercial Application generally.
For strengthening mouthfeel, add the ratio 8%～15% that described superoxide dismutase dry powder accounts for gross mass during preparation, all the other food additive total amounts for adding.
Compare with prior art, the present invention has the following advantages:
1, SOD is a kind of to the very sensitive metalloenzyme of pH value, can inactivation under sour environment.It is soluble in water, and passes through mucosa absorption easily.Of the present invention SOD dry powder is made the SOD buccal tablet, buccal tablet is when taking, SOD progressively discharges its activity by the slow dissolving of saliva, fully absorbed by oral mucosa and esophageal mucosa membrane injury again, thereby avoided SOD to enter stomach and under gastric acid environment the phenomenon of inactivation, directly enter human body and give full play to its special efficacy.
2, the SOD dry powder among the present invention is to extract from Semen Maydis.In leaching process, do not add any chemical substance that is harmful to health, make that SOD maximizes the retention in the Semen Maydis.As everyone knows, material extracts more pure, and its cost is more high.The price of the pure product of SOD is 1～1.5 ten thousand dollar/grams on the international market, and the price of the pure product of SOD is 5.9～120,000 RMB/grams on the domestic market.Because the costliness of SOD has limited its comprehensive popularization to a great extent.Among the present invention with the made SOD buccal tablet price of corn SOD dry powder well below the product price of utilizing the pure product of SOD to produce, can be accepted by the numerous consumer groups.
The specific embodiment
Embodiment 1
A kind of superoxide dismutase oral buccal tablet, formed by superoxide dismutase and food additive, every buccal tablet net weight 680mg contains 2.4 ten thousand U active superoxide dismutase in every tablet of buccal tablet, namely contain 3.5 ten thousand U active superoxide dismutase approximately in every gram buccal tablet; Food additive comprises xylitol, hydroxypropyl fiber, magnesium stearate, silicon dioxide and microcrystalline cellulose, and the additive total amount accounts for buccal tablet gross mass 84%.
Embodiment 2
The preparation method of the superoxide dismutase oral buccal tablet of embodiment 1, with 25g, activity is the superoxide dismutase dry powder of 300,000 U/g and the food additive of following food stage purity: after 75g xylitol, 28g hydroxypropyl fiber, 11g magnesium stearate, 9g silicon dioxide, 63g microcrystalline cellulose fully mix, be divided into buccal tablet with tablet machine, make that the quality of every tablet of buccal tablet is 680mg, aseptic packaging gets final product again.
Embodiment 3
Superoxide dismutase dry powder among the embodiment 2 adopts following steps to make:
The first step: get corn distiller's dried grain 120Kg, clean, behind warm water (30 ℃) immersion 24h, taking-up drains, and obtains wet corn 140Kg.This Semen Maydis is added 180L water mill slurry, obtain containing the Semen Maydis serosity 350L of superoxide dismutase.
Second step: regulate pH to 7～9 with ammonia behind the 24.5g anhydrous cupric sulfate in above-mentioned Semen Maydis serosity, carry out solid-liquid separation by filter pressing, obtaining superoxide dismutase activity is the proenzyme liquid 210L of 468U/mL.
The 3rd step: will go up the superoxide dismutase stock solution that obtains of step and concentrate by ceramic membrane filter and ultrafilter membrane successively, ceramic membrane is removed molecular weight〉120,000 impurity, ultrafilter membrane is then removed the impurity of molecular weight＜10,000, obtain the superoxide dismutase concentrated solution, the activity of superoxide dismutase is 9691U/mL in the concentrated solution, volume is 10L, and active conservation rate is 98.6%.
The 4th step: upwards go on foot in the superoxide dismutase concentrated solution of gained and add acetone, obtain precipitate through separation, the above-mentioned precipitate of lyophilization namely obtains required dry powder.
Embodiment 4
A kind of superoxide dismutase oral buccal tablet, formed by superoxide dismutase and food additive, every buccal tablet net weight 680mg contains 1.5 ten thousand U active superoxide dismutase in every tablet of buccal tablet, namely contain 2.2 ten thousand U active superoxide dismutase approximately in every gram buccal tablet; Food additive comprises mannitol, hydroxypropyl amide, sodium stearate, silicon dioxide and microcrystalline cellulose, and the additive total amount accounts for buccal tablet gross mass 93%.
Embodiment 5
The preparation method of the superoxide dismutase oral buccal tablet of embodiment 4, with 25g, activity is the superoxide dismutase dry powder of 300,000 U/g and the food additive of following food stage purity: after 150g xylitol, 56g hydroxypropyl fiber, 18g magnesium stearate, 6g silicon dioxide, 82g microcrystalline cellulose fully mix, be divided into buccal tablet with tablet machine, make that the quality of every tablet of buccal tablet is 680mg, aseptic packaging gets final product again.
Embodiment 6
A kind of superoxide dismutase oral buccal tablet, formed by superoxide dismutase and food additive, every buccal tablet net weight 680mg contains 3.1 ten thousand U active superoxide dismutase in every tablet of buccal tablet, namely contain 4.5 ten thousand U active superoxide dismutase approximately in every gram buccal tablet; Food additive comprises mannitol, hydroxypropyl amide, sodium stearate, silicon dioxide and microcrystalline cellulose, and the additive total amount accounts for buccal tablet gross mass 93%.
Embodiment 7
The preparation method of the superoxide dismutase oral buccal tablet of embodiment 6, with 30g, activity is the superoxide dismutase dry powder of 500,000 U/g and the food additive of following food stage purity: after 150g xylitol, 56g hydroxypropyl fiber, 18g magnesium stearate, 6g silicon dioxide, 82g microcrystalline cellulose fully mix, be divided into buccal tablet with tablet machine, make that the quality of every tablet of buccal tablet is 680mg, aseptic packaging gets final product again.
Embodiment 8
Superoxide dismutase dry powder among the embodiment 7 adopts following steps to make:
The first step: get corn distiller's dried grain 120Kg, clean, behind warm water (30 ℃) immersion 24h, taking-up drains, and obtains wet corn 140Kg.This Semen Maydis is added 180L water mill slurry, obtain containing the Semen Maydis serosity 350L of superoxide dismutase.
Second step: regulate pH to 7～9 with ammonia behind the 24.5g anhydrous cupric sulfate in above-mentioned Semen Maydis serosity, carry out solid-liquid separation by filter pressing, obtaining superoxide dismutase activity is the proenzyme liquid 210L of 468U/mL.
The 3rd step: will go up the superoxide dismutase stock solution that obtains of step and concentrate by ceramic membrane filter and ultrafilter membrane successively, ceramic membrane is removed molecular weight〉120,000 impurity, ultrafilter membrane is then removed the impurity of molecular weight＜10,000, obtain the superoxide dismutase concentrated solution, the activity of superoxide dismutase is 9691U/mL in the concentrated solution, volume is 10L, and active conservation rate is 98.6%.
The 4th step: upwards go on foot in the superoxide dismutase concentrated solution of gained and add acetone, obtain precipitate through separation, the above-mentioned precipitate of lyophilization gets elementary dry powder.
The 5th step: after the elementary dry powder of the 4th step gained is water-soluble, add acetone again, obtain precipitate through separation, the above-mentioned precipitate of lyophilization makes superoxide dismutase dry powder.
Claims (8)
1. a superoxide dismutase oral buccal tablet comprises superoxide dismutase and food additive, it is characterized in that: contain 1.4～4.5 ten thousand U active superoxide dismutase in the oral buccal tablet of every gram.
2. superoxide dismutase oral buccal tablet as claimed in claim 1, it is characterized in that: food additive accounts for 80%～90% of buccal tablet gross mass.
3. the preparation method of a superoxide dismutase oral buccal tablet as claimed in claim 1 or 2, it is characterized in that: getting an amount of activity is the superoxide dismutase dry powder of 10U/g～500,000 U/g, after food additive is mixed, compression molding.
4. the preparation method of superoxide dismutase oral buccal tablet as claimed in claim 3 is characterized in that: described superoxide dismutase dry powder adopts following steps to make,
The first step: Semen Maydis is carried out pretreatment obtain the Semen Maydis serosity;
Second step: in the Semen Maydis serosity, add water the dissolubility mantoquita, keep pH value 7～9, afterwards through solid-liquid separation, obtain superoxide dismutase stock solution;
The 3rd step: with the superoxide dismutase stock solution membrane filtration more than two-stage again of the second step gained, remove molecular weight successively〉120,000 and the impurity of molecular weight＜10,000, obtain the superoxide dismutase concentrated solution;
The 4th step: in the superoxide dismutase concentrated solution, add and to obtain precipitate with the Organic substance liquid that water dissolves each other through separating, the described precipitate of lyophilization.
5. the preparation method of superoxide dismutase oral buccal tablet as claimed in claim 4, it is characterized in that: after described the 4th step gained dry powder is water-soluble again, add again and can obtain precipitate with the Organic substance liquid that water dissolves each other through separating, the described precipitate of lyophilization.
6. as the preparation method of claim 4 or 5 described superoxide dismutase oral buccal tablets, it is characterized in that: the described preferred acetone of Organic substance liquid, dehydrated alcohol or the methanol that can dissolve each other with water.
7. as the preparation method of claim 4 or 5 described superoxide dismutase oral buccal tablets, it is characterized in that: add the ratio 8%～15% that described superoxide dismutase dry powder accounts for gross mass during preparation.
8. the preparation method of superoxide dismutase oral buccal tablet as claimed in claim 6 is characterized in that: add the ratio 8%～15% that described superoxide dismutase dry powder accounts for gross mass during preparation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN2013101062778A CN103191070A (en) | 2013-03-29 | 2013-03-29 | Oral tablet of superoxide dismutase and preparation method thereof |
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| Application Number | Priority Date | Filing Date | Title |
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| CN2013101062778A CN103191070A (en) | 2013-03-29 | 2013-03-29 | Oral tablet of superoxide dismutase and preparation method thereof |
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| CN103191070A true CN103191070A (en) | 2013-07-10 |
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| CN2013101062778A Pending CN103191070A (en) | 2013-03-29 | 2013-03-29 | Oral tablet of superoxide dismutase and preparation method thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104774812A (en) * | 2014-10-09 | 2015-07-15 | 湖南一九生物科技有限公司 | Processing method for extraction of superoxide dismutase from corn germ |
| CN110075280A (en) * | 2019-04-30 | 2019-08-02 | 王丛飞 | A kind of compound oral agents of laminin peptide and preparation method thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4842846A (en) * | 1986-11-22 | 1989-06-27 | Minoru Nakano | Superoxide dismutase composition for periodontal use |
| CN1387913A (en) * | 2001-05-25 | 2003-01-01 | 义乌市金达康生物制品有限公司 | Buccal tablet containing SOD |
| CN1570095A (en) * | 2004-05-09 | 2005-01-26 | 沈阳五爱康田生物制品有限责任公司 | Superoxide dismutase complex enzyme industrialized production method using corn as material and its special device for germination |
| CN102517264A (en) * | 2011-12-28 | 2012-06-27 | 湖南大学 | Method for extracting superoxide dismutase from plants |
-
2013
- 2013-03-29 CN CN2013101062778A patent/CN103191070A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4842846A (en) * | 1986-11-22 | 1989-06-27 | Minoru Nakano | Superoxide dismutase composition for periodontal use |
| CN1387913A (en) * | 2001-05-25 | 2003-01-01 | 义乌市金达康生物制品有限公司 | Buccal tablet containing SOD |
| CN1570095A (en) * | 2004-05-09 | 2005-01-26 | 沈阳五爱康田生物制品有限责任公司 | Superoxide dismutase complex enzyme industrialized production method using corn as material and its special device for germination |
| CN102517264A (en) * | 2011-12-28 | 2012-06-27 | 湖南大学 | Method for extracting superoxide dismutase from plants |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104774812A (en) * | 2014-10-09 | 2015-07-15 | 湖南一九生物科技有限公司 | Processing method for extraction of superoxide dismutase from corn germ |
| CN110075280A (en) * | 2019-04-30 | 2019-08-02 | 王丛飞 | A kind of compound oral agents of laminin peptide and preparation method thereof |
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Application publication date: 20130710 |