CN104770398A - Biocontrol bacterium and trifloxystrobin composition - Google Patents
Biocontrol bacterium and trifloxystrobin composition Download PDFInfo
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- CN104770398A CN104770398A CN201510042877.1A CN201510042877A CN104770398A CN 104770398 A CN104770398 A CN 104770398A CN 201510042877 A CN201510042877 A CN 201510042877A CN 104770398 A CN104770398 A CN 104770398A
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- oxime bacterium
- bacillus subtilis
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Abstract
The invention discloses a biocontrol bacterium and trifloxystrobin composition, and belongs to the technical field of bactericides. The composition contains the following active components: Bacillus subtilis and trifloxystrobin. The composition can effectively control rice sheath blight, rice false smut, rice blast, seedling blight, tomato late blight, tomato botrytis, wheat powdery mildew, wheat sheath blight, cotton Rhizoctonia solani Kuhn, cotton fusarium wilt, cotton verticillium wilt, soybean root rot, corn sheath blight, cucumber botrytis, cucumber wilt, tobacco brown spot, tobacco bacterial wilt disease, various field and garden crop root rot, seedling blight and other various diseases, and substantially reduces the pesticide cost, so the pesticide cost is reduced, the control range is enlarged, environmental pollution is reduced, and the pesticide harm and the pesticide resistance risk are reduced.
Description
Technical field
The invention belongs to bactericide technical field, be specifically related to the composition of a kind of biocontrol bacteria and oxime bacterium ester.
Background technology
Bacillus subtilis is the one in bacillus, extensively distributes in natural environment, has application to be a kind of beneficial microbe at industrial agriculture field more.This bacterium of plant protection field mainly by competition, bacteriolyze, secretion antibiotics, induction of resistance, the mechanisms of action such as short host's growth are to multiple Fusarium, aspergillus, the multiple fungi of Alternaria and Rhizoctonia has Biocontrol Effect, to rice sheath blight disease, rice green smut, rice blast, rice seedling damping off, tomato late blight, graw mold of tomato, wheat powdery mildew, wheat sharp eyespot, cotton rhizoctonia solani, cotton-wilt fusarium, cotton verticillium wilt, root rot, maize sheath blight, gray mold of cucumber, cucumber fusarium axysporum, Alternaria alternate, tobacco bacterial wilt, all kinds of land for growing field crops and horticultural crop root rot, the diseases such as damping off have fine control efficiency.In addition its to person poultry safety, conform, pollute relatively little, be easy to the plurality of advantages such as fermentation, make this bacterium become one of important biocontrol microorganisms.The purposes of this bacterium in plant protection field is more and more extensive, and all carried out large quantifier elimination to this bacterium both at home and abroad, the commercialization of multiple bacterial strain also obtains extensive use.The U.S. oneself have 4 strain bacillus subtilis biocontrol bacterial strains (QST713, MB1600, GB03 and FZB24) obtain Environmental Protection Agency's (EPA) commercialization or limited merchandized handling application license.China has also successfully developed hundred anti-, wheat Fengning, line Qu Ning, sub-multiple bacillus subtilis commercial formulations such as precious, and obtains propagation and employment widely, achieves good economic and social benefit.
Oxime bacterium ester (Trifloxystrobin), chemical name is: (2Z)-2-methoxyimino-2-[2-[[1-[3-(trifluoromethyl) phenyl] ethyleneimino] oxygen methyl] phenyl] methyl acetate), it is the fungicides that a kind of class successfully developed as bactericide lead compound from natural products Strobilurins is new, have efficient, wide spectrum, the good characteristics such as infiltration, Crop is fast, in addition it has interior absorption upwards, therefore resistance of rainwater washing against performance is good, lasting period is long, therefore second generation methoxy acrylic bactericide is considered to.Be mainly used in cauline leaf process, prolection is excellent, and has certain therapeutic activity, and activity is not affected by environment, and application optimal period is spore germination and their early stage stage.Good activity is all had to nearly all Mycophytes (Ascomycetes, Basidiomycetes, Oomycete and Fungi Imperfecti) disease such as powdery mildew, rust, Ying's rot, net blotch, downy mildew, rice blast etc.Except having special efficacy to powdery mildew, leaf spot, there is good activity to rust, downy mildew, damping off, apple apple scab, sclerotinia rot of colza.
Although oxime bacterium ester has, efficient, quick-acting, interior absorption is strong, the advantage of resistance of rainwater washing against, also there is many defects such as resistance risk, production cost height.Bacillus subtilis, as a kind of biopesticide, has environmental friendliness, and production cost is low, not easily produces the advantage of resistance and lasting period length, but also there is effective slow, high to requirement for environmental conditions shortcoming.
Therefore, the pluses and minuses of bacillus subtilis and oxime bacterium ester have good complementarity, and bacillus subtilis belongs to prokaryotes in addition, does not have mitochondria, not by the suppression of oxime bacterium ester.Both combinations are passable, can greatly reduce the consumption of oxime bacterium ester, thus reduce drug cost, expanding prevention scope, and can reduce environmental pollution, reduce poisoning and resistance risk.
Summary of the invention
For prior art Problems existing, the object of the invention is to design the technical scheme of the composition that a kind of biocontrol bacteria and oxime bacterium ester are provided.
The composition of described a kind of biocontrol bacteria and oxime bacterium ester, is characterized in that containing active component bacillus subtilis and oxime bacterium ester.
The composition of described a kind of biocontrol bacteria and oxime bacterium ester, is characterized in that the proportional quantity of described bacillus subtilis and oxime bacterium ester is 1 gram of oxime bacterium ester: 0.001 hundred million ~ 10,000 hundred million bacillus subtilis spore.
The composition of described a kind of biocontrol bacteria and oxime bacterium ester, is characterized in that the proportional quantity of described bacillus subtilis and oxime bacterium ester is 1 gram of oxime bacterium ester: 5,000,000,000 ~ 1,000 hundred million bacillus subtilis spores.
The composition of described a kind of biocontrol bacteria and oxime bacterium ester, is characterized in that containing accounting for the bacillus subtilis of composition total weight 5 ~ 35% and account for the oxime bacterium ester of composition total weight 5 ~ 35%, described bacillus subtilis spore content is 20,000,000,000/gram.
The composition of described a kind of biocontrol bacteria and oxime bacterium ester, is characterized in that containing accounting for the bacillus subtilis of composition total weight 10 ~ 20% and account for the oxime bacterium ester of composition total weight 12.5 ~ 30%, described bacillus subtilis spore content is 20,000,000,000/gram.
The application in the water prevention rice sheath blight, false smut, rice blast, seedling blight, tomato late blight, graw mold of tomato, wheat powdery mildew, wheat sharp eyespot, cotton rhizoctonia solani, cotton-wilt fusarium, cotton verticillium wilt, root rot, maize sheath blight, gray mold of cucumber, cucumber fusarium axysporum, Alternaria alternate, tobacco bacterial wilt, all kinds of land for growing field crops and horticultural crop root rot, damping off of the composition of described a kind of biocontrol bacteria and oxime bacterium ester.
Composition of the present invention in effective prevention and control to rice sheath blight disease, false smut, rice blast, seedling blight, tomato late blight, graw mold of tomato, wheat powdery mildew, wheat sharp eyespot, cotton rhizoctonia solani, cotton-wilt fusarium, cotton verticillium wilt, root rot, maize sheath blight, gray mold of cucumber, cucumber fusarium axysporum, Alternaria alternate, tobacco bacterial wilt, all kinds of land for growing field crops and horticultural crop root rot, while the multiple diseases such as damping off, remarkable reduction drug cost, thus reduction drug cost, expanding prevention scope, and can environmental pollution be reduced, reduce poisoning and resistance risk.
Embodiment
The present invention is further illustrated below in conjunction with embodiment.
Composition of the present invention can add conven-tional adjuvants, and through common process, be made into the formulation that granule, wetting powder, water dispersible granules, microcapsule formulations etc. are conventional, method of application can for spreading fertilizer over the fields, spraying, fill with root and seed dressing.
Test example 1 oxime bacterium ester is on the impact of bacillus subtilis.
The bacillus subtilis activated is linked in the LB medium containing oxime bacterium ester 0.1mg/L, 0.2mg/L, 0.4mg/L, 0.8mg/L, 1.6mg/L and blank, 30 DEG C, 150r/min cultivates 24h, adopts the viable bacteria content of each concentration of plate dilution assay method.
The survey showed that, and oxime bacterium ester does not only have inhibitory action to bacillus subtilis, and have certain stimulating growth effect, and therefore both are mixed can not cause adverse effect to the vitality of bacillus subtilis.
The composition of test examples 1 oxime bacterium ester and bacillus subtilis is to the control effect of rice sheath blight disease
Test Summary and result as shown in the table:
As can be seen from result of the test, under the condition that active ingredient is almost identical, the composition of bacillus subtilis and oxime bacterium ester substantially increases preventive effect, and far above bacillus subtilis and two the single dose process of oxime bacterium ester in preventive effect, the combination of both explanations has drug effect synergistic effect.
The composition of test examples 2 oxime bacterium ester and bacillus subtilis is to the control effect of wheat scab
Test Summary and result as shown in the table:
As can be seen from result of the test, under the condition that active ingredient is almost identical, far above bacillus subtilis and two the single dose process of oxime bacterium ester in the composition preventive effect of bacillus subtilis and oxime bacterium ester, the preventive effect that can substantially increase wheat scab is combined in both explanations, and the combination of both explanations has drug effect synergistic effect.
The composition of test examples 3 oxime bacterium ester and bacillus subtilis is to the control effect of sclerotinia rot of colza
Test Summary and result as shown in the table:
As can be seen from result of the test, under the condition that active ingredient is almost identical, far above bacillus subtilis and two the single dose process of oxime bacterium ester in the composition preventive effect of bacillus subtilis and oxime bacterium ester, the preventive effect that can substantially increase sclerotinia rot of colza is combined in both explanations, and the combination of both explanations has drug effect synergistic effect.
The synergistic effect of composition in rice green smut control of test examples 4 oxime bacterium ester and bacillus subtilis
Test Summary and result as shown in the table:
As can be seen from result of the test, under the condition that active ingredient is almost identical, the composition of bacillus subtilis and oxime bacterium ester in preventive effect far above bacillus subtilis and two the single dose process of oxime bacterium ester, the preventive effect that can substantially increase rice green smut is combined in both explanations, and the combination of both explanations has drug effect synergistic effect.
The synergistic effect of composition in rice blast control of test examples 5 oxime bacterium ester and bacillus subtilis
Test Summary and result as shown in the table:
The composition of test examples 6 oxime bacterium ester and bacillus subtilis is to rice sheath blight disease, rice green smut, rice blast, rice seedling damping off, tomato late blight, graw mold of tomato, grey mould fruit rot of strawberry, wheat powdery mildew, wheat sharp eyespot, cotton rhizoctonia solani, cotton-wilt fusarium, cotton verticillium wilt, root rot, maize sheath blight, gray mold of cucumber, cucumber fusarium axysporum, the control of the multiple diseases such as Alternaria alternate, tobacco bacterial wilt all shows the synergistic effect in preventive effect, do not repeat them here.
Claims (6)
1. a composition for biocontrol bacteria and oxime bacterium ester, is characterized in that containing active component bacillus subtilis and oxime bacterium ester.
2. the composition of a kind of biocontrol bacteria as claimed in claim 1 and oxime bacterium ester, is characterized in that the proportional quantity of described bacillus subtilis and oxime bacterium ester is 1 gram of oxime bacterium ester: 0.001 hundred million ~ 10,000 hundred million bacillus subtilis spore.
3. the composition of a kind of biocontrol bacteria as claimed in claim 1 and oxime bacterium ester, is characterized in that the proportional quantity of described bacillus subtilis and oxime bacterium ester is 1 gram of oxime bacterium ester: 5,000,000,000 ~ 1,000 hundred million bacillus subtilis spores.
4. the composition of a kind of biocontrol bacteria as claimed in claim 1 and oxime bacterium ester, it is characterized in that containing accounting for the bacillus subtilis of composition total weight 5 ~ 35% and account for the oxime bacterium ester of composition total weight 5 ~ 35%, described bacillus subtilis spore content is 20,000,000,000/gram.
5. the composition of a kind of biocontrol bacteria as claimed in claim 1 and oxime bacterium ester, it is characterized in that containing accounting for the bacillus subtilis of composition total weight 10 ~ 20% and account for the oxime bacterium ester of composition total weight 12.5 ~ 30%, described bacillus subtilis spore content is 20,000,000,000/gram.
6. the application of composition in the water prevention rice sheath blight, false smut, rice blast, seedling blight, tomato late blight, graw mold of tomato, wheat powdery mildew, wheat sharp eyespot, cotton rhizoctonia solani, cotton-wilt fusarium, cotton verticillium wilt, root rot, maize sheath blight, gray mold of cucumber, cucumber fusarium axysporum, Alternaria alternate, tobacco bacterial wilt, all kinds of land for growing field crops and horticultural crop root rot, damping off of a kind of biocontrol bacteria as claimed in claim 1 and oxime bacterium ester.
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| CN201510042877.1A CN104770398B (en) | 2015-01-28 | 2015-01-28 | A kind of composition of biocontrol bacteria and trifloxystrobin |
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| CN201510042877.1A CN104770398B (en) | 2015-01-28 | 2015-01-28 | A kind of composition of biocontrol bacteria and trifloxystrobin |
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| CN104770398A true CN104770398A (en) | 2015-07-15 |
| CN104770398B CN104770398B (en) | 2018-04-10 |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101990398A (en) * | 2008-04-07 | 2011-03-23 | 拜耳作物科学有限公司 | Stable aqueous spore-containing formulation |
| CN103260410A (en) * | 2010-12-08 | 2013-08-21 | 巴斯夫欧洲公司 | Pesticidal mixtures |
| WO2013178650A1 (en) * | 2012-05-30 | 2013-12-05 | Bayer Cropscience Ag | Composition comprising a biological control agent and a fungicide selected from inhibitors of the respiratory chain at complex iii |
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- 2015-01-28 CN CN201510042877.1A patent/CN104770398B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101990398A (en) * | 2008-04-07 | 2011-03-23 | 拜耳作物科学有限公司 | Stable aqueous spore-containing formulation |
| CN103260410A (en) * | 2010-12-08 | 2013-08-21 | 巴斯夫欧洲公司 | Pesticidal mixtures |
| WO2013178650A1 (en) * | 2012-05-30 | 2013-12-05 | Bayer Cropscience Ag | Composition comprising a biological control agent and a fungicide selected from inhibitors of the respiratory chain at complex iii |
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| CN104770398B (en) | 2018-04-10 |
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