CN104756753A - Artificial cultivation method of cordyceps sobolifera - Google Patents
Artificial cultivation method of cordyceps sobolifera Download PDFInfo
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- CN104756753A CN104756753A CN201510039103.3A CN201510039103A CN104756753A CN 104756753 A CN104756753 A CN 104756753A CN 201510039103 A CN201510039103 A CN 201510039103A CN 104756753 A CN104756753 A CN 104756753A
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- cicada
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- cordyceps sobolifera
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Abstract
The invention discloses an artificial cultivation method of cordyceps sobolifera. The method comprises the following steps: soaking mature nymphs of cicadas obtained by either field acquisition or artificial cultivation with pure water for 5 min, washing, and drying on a filter screen; uniformly spraying paecilomyces cicadae strain diluted solution, of which the total active bacteria number is 1*108, obtained by the culture and expanding propagation in a culture chamber, to the dried nymphs by a sprayer according to 5% of the weight of the nymphs; moving the re-dried nymphs into a cordyceps sobolifera cultivation plate, covering a plate cover, and cultivating in a sterile room at the temperature of 24-26 DEG C and the relative air humidity of 80-90%; after the heads of the nymphs grow cordyceps sobolifera which is similar to flowers, and taking out and drying the cordyceps sobolifera to obtain the artificially cultivated cordyceps sobolifera. Meanwhile, the invention discloses a cultivation plate for artificially cultivating the cordyceps sobolifera; in addition, by adopting the artificial cultivation method of cordyceps sobolifera to cultivate the cordyceps sobolifera, the industrial production of the cordyceps sobolifera can be realized, and the increasingly grown dietary therapy and clinical demands can be met.
Description
Technical field
The present invention relates to Chinese caterpillar fungus artificial cultivation technique field, the artificial cultivation method of especially a kind of cicada fungus.
Background technology
Cicada fungus belongs to worm natural disposition medicinal fungi, is that Paecilomyces cicadae bacterium colonizes in the entomogenous fungi complex that the nymph of Homoptera cicada is formed.Modern scientific research shows, cicada fungus is applied to the field such as control of male sexual disorder, cancer of pancreas, cervical carcinoma, cancer of the stomach, leukemia, insomnia, diabetes and complication thereof, there is effect similar with Cordyceps sinensis, and its content of beary metal is also lower than Cordyceps sinensis, there is very large exploitation, value.
the nymph of cicada lives in soil, and sucking plant root juice, whole nymph reaches 2-16.Under field conditions (factors), need specific ecotope, nymph just can be infected by Paecilomyces cicadae bacterium.The natural resources of cicada fungus is very rare, and add unordered for a long time excavating, natural production reduces year by year, and resource is increasingly exhausted, can not meet growing dietotherapy and clinical demand.So far also there is no the report of artificial cultivation cicada fungus both at home and abroad, be not applicable to the relevant appliance of artificial cultivation cicada fungus yet.
Summary of the invention
The object of this invention is to provide a kind of method of artificial cultivation cicada fungus, to meet growing dietotherapy and clinical demand, realize industrialization and produce.
technical scheme of the present invention is: the artificial cultivation method of a kind of cicada fungus, adopts following steps cultivation to form:
A. from the ripe nymph of field acquisition cicada or propagate the ripe nymph obtaining cicada artificially;
B. with pure water polypide soaked 5 minutes and rinse well, being placed on filter screen and draining;
C. adopt in bacterial classification room liquid nutrient medium to cultivate, expand numerous Paecilomyces cicadae bacterium, obtain Paecilomyces cicadae bacterium bacterial classification;
D. Paecilomyces cicadae bacterium bacterial classification pure water is diluted, obtained total viable count 1 × 10
8paecilomyces cicadae bacterium bacterial classification dilution;
E. on the polypide adopting sprayer to be evenly sprayed at by Paecilomyces cicadae bacterium bacterial classification dilution to drain by 5% of body weight;
F. the polypide of being infected with Paecilomyces cicadae bacterium is reapposed on filter screen and drains;
G. the polypide again drained is moved in cicada fungus dish for cultivating, build disk cover;
H. in aseptic indoor growing, set temperature is 24-26 ° of C, and relative air humidity is 80-90%;
I. treat that polypide head grows the cicada fungus of likeness in form flower, can take out;
J. the cicada fungus of taking-up is dried in time at the temperature of 55-75 ° of C, pack by weight, namely obtain tame cicada fungus.
: in the season of the nymph maturation of cicada catch at the host plant base portion of cicada the ripe nymph just getting out, prepare to climb up host plant stem from soil from the method for the ripe nymph of field acquisition cicada.
The method propagating the ripe nymph of cicada artificially is:
A. from the branch of field acquisition band cicada ovum, every bar branch has 50-100 grain cicada ovum;
B. by being with, the branch of cicada ovum is placed on temperature 20-28 ° of C, the indoor of relative moisture 65-70% carry out hatching process for one month;
C. radially excavate shallow ridges at the base portion of host plant, the branch of band cicada ovum is nuzzled in soil;
D. catch for subsequent use when the nymph of cicada is ripe in time.
The method that numerous Paecilomyces cicadae bacterium bacterial classification was cultivated, expanded in bacterial classification room is: adopt Richard medium to carry out liquid culture, and early stage, temperature was 24-26 DEG C, and pH value is 5-6, can obtain conidium sum and reach 3.9 × 10 after 14 days
11the Paecilomyces cicadae bacterium bacterial classification of individual/ml.
Described cicada fungus dish for cultivating comprises by the circular disk body of transparent glass or plastic processing and disk cover, main feature of the present invention is, described disk cover is provided with the circular hole of band boss, described boss is provided with circle support, is provided with ePTFE varicosity politef water proof and dust proof ventilated membrane in described circle support; The inner face of described disk cover and the dish mouth contact surface of circular disk body are provided with circular seal ring; The tray bottom of described circular disk body is provided with circular water suction pad; The diameter of described circular disk body is 5-50cm, is highly 5-10cm.
Adopt the method cultivation cicada fungus of artificial cultivation cicada fungus provided by the invention, the industrialization that can realize cicada fungus is produced, and meets growing dietotherapy and clinical demand.
Accompanying drawing explanation
Concrete structure of the present invention is provided by following embodiment and accompanying drawing thereof.
Fig. 1 is the structural representation of concrete structure of the cicada fungus dish for cultivating proposed according to the present invention.
In figure, (1) is boss, and (2) are circular hole, and (3) are ePTFE varicosity politef water proof and dust proof ventilated membrane, and (4) are circle support, and (5) are disk cover, and (6) are circular seal ring, and (7) are circular disk body, and (8) are circular water suction pad.
Embodiment
Below in conjunction with embodiment, the present invention is described further, and wherein said raw material number, except specified otherwise, is parts by weight.
ripe nymph from field acquisition cicada: in the season of the nymph maturation of cicada, catches at the host plant base portion of cicada the ripe nymph just getting out, prepare to climb up host plant stem from soil.
propagate the ripe nymph of cicada artificially:
A. from the branch of field acquisition band cicada ovum, every bar branch has 50-100 grain cicada ovum;
B. by being with, the branch of cicada ovum is placed on temperature 20-28 ° of C, the indoor of relative moisture 65-70% carry out hatching process for one month;
C. radially excavate shallow ridges at the base portion of host plant, the branch of band cicada ovum is nuzzled in soil;
D. catch for subsequent use when the nymph of cicada is ripe in time.
Bacterial classification room is cultivated, is expanded numerous Paecilomyces cicadae bacterium bacterial classification: adopt Richard medium to carry out liquid culture, and early stage, temperature was 24-26 DEG C, and pH value is 5-6, can obtain conidium sum and reach 3.9 × 10 after 14 days
11the Paecilomyces cicadae bacterium bacterial classification of individual/ml.
Embodiment
:following steps cultivation is adopted to form:
A. from the ripe nymph of field acquisition cicada or propagate the ripe nymph obtaining cicada artificially;
B. with pure water polypide soaked 5 minutes and rinse well, being placed on filter screen and draining;
C. adopt in bacterial classification room liquid nutrient medium to cultivate, expand numerous Paecilomyces cicadae bacterium, obtain Paecilomyces cicadae bacterium bacterial classification;
D. Paecilomyces cicadae bacterium bacterial classification pure water is diluted, obtained conidium sum 1 × 10
8the Paecilomyces cicadae bacterium bacterial classification dilution of individual/ml;
E. on the polypide adopting sprayer to be evenly sprayed at by Paecilomyces cicadae bacterium bacterial classification dilution to drain by 5% of body weight;
F. the polypide of being infected with Paecilomyces cicadae bacterium is reapposed on filter screen and drains;
G. the polypide again drained is moved in cicada fungus dish for cultivating, build disk cover;
H. in aseptic indoor growing, set temperature is 24-26 ° of C, and relative air humidity is 80-90%;
I. treat that polypide head grows the cicada fungus of likeness in form flower, can take out;
J. the cicada fungus of taking-up is dried in time at the temperature of 55-75 ° of C, pack by weight, namely obtain tame cicada fungus.
The cicada fungus dish for cultivating that the present invention adopts comprises by the circular disk body (7) of transparent glass or plastic processing and disk cover (5), main feature of the present invention is, disk cover (5) is provided with the circular hole (2) of band boss (1), boss (1) is provided with circle support (4), is provided with ePTFE varicosity politef water proof and dust proof ventilated membrane (3) in circle support (4); The inner face of disk cover (5) and the dish mouth contact surface of circular disk body (7) are provided with circular seal ring (6); The tray bottom of circular disk body (7) is provided with circular water suction pad (8); The diameter of circular disk body (7) is 10cm, is highly 6cm.
Claims (5)
1. an artificial cultivation method for cicada fungus, is characterized in that, adopts following steps cultivation to form:
A. from the ripe nymph of field acquisition cicada or propagate the ripe nymph obtaining cicada artificially;
B. with pure water polypide soaked 5 minutes and rinse well, being placed on filter screen and draining;
C. adopt in bacterial classification room liquid nutrient medium to cultivate, expand numerous Paecilomyces cicadae bacterium, obtain Paecilomyces cicadae bacterium bacterial classification;
D. Paecilomyces cicadae bacterium bacterial classification pure water is diluted, obtained conidium sum 1 × 10
8the Paecilomyces cicadae bacterium bacterial classification dilution of individual/ml;
E. on the polypide adopting sprayer to be evenly sprayed at by Paecilomyces cicadae bacterium bacterial classification dilution to drain by 5% of body weight;
F. the polypide of being infected with Paecilomyces cicadae bacterium is reapposed on filter screen and drains;
G. the polypide again drained is moved in cicada fungus dish for cultivating, build disk cover;
H. in aseptic indoor growing, set temperature is 24-26 ° of C, and relative air humidity is 80-90%;
I. treat that polypide head grows the cicada fungus of likeness in form flower, can take out;
J. the cicada fungus of taking-up is dried in time at the temperature of 55-75 ° of C, pack by weight, namely obtain tame cicada fungus.
2. according to the artificial cultivation method of a kind of cicada fungus described in claim 1, it is characterized in that,: in the season of the nymph maturation of cicada catch at the host plant base portion of cicada the ripe nymph just getting out, prepare to climb up host plant stem from soil from the method for the ripe nymph of field acquisition cicada.
3. according to the artificial cultivation method of a kind of cicada fungus described in claim 1, it is characterized in that, the method propagating the ripe nymph of cicada artificially is:
A. from the branch of field acquisition band cicada ovum, every bar branch has 50-100 grain cicada ovum;
B. by being with, the branch of cicada ovum is placed on temperature 20-28 ° of C, the indoor of relative moisture 65-70% carry out hatching process for one month;
C. radially excavate shallow ridges at the base portion of host plant, the branch of band cicada ovum is nuzzled in soil;
D. catch for subsequent use when the nymph of cicada is ripe in time.
4. according to the artificial cultivation method of a kind of cicada fungus described in claim 1, it is characterized in that, the method that numerous Paecilomyces cicadae bacterium bacterial classification was cultivated, expanded in bacterial classification room is: adopt Richard medium to carry out liquid culture, early stage, temperature was 24-26 DEG C, pH value is 5-6, can obtain conidium sum and reach 3.9 × 10 after 14 days
11the Paecilomyces cicadae bacterium bacterial classification of individual/ml.
5. according to the artificial cultivation method of a kind of cicada fungus described in claim 1, it is characterized in that, cicada fungus dish for cultivating comprises by the circular disk body (7) of transparent glass or plastic processing and disk cover (5), main feature of the present invention is, described disk cover (5) is provided with the circular hole (2) of band boss (1), described boss (1) is provided with circle support (4), is provided with ePTFE varicosity politef water proof and dust proof ventilated membrane (3) in described circle support (4);
The inner face of described disk cover (5) and the dish mouth contact surface of circular disk body (7) are provided with circular seal ring (6);
The tray bottom of described circular disk body (7) is provided with circular water suction pad (8);
The diameter of described circular disk body (7) is 5-50cm, is highly 5-10cm.
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Cited By (6)
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| CN106242682A (en) * | 2016-08-04 | 2016-12-21 | 贵州宏华农业发展有限公司 | Agaricus blazei Murrill Primary spawn material and manufacture method |
| WO2017202293A1 (en) * | 2016-05-23 | 2017-11-30 | 浙江泛亚生物医药股份有限公司 | Artificial cultivation method for cordyceps sobolifera |
| CN109247188A (en) * | 2018-08-29 | 2019-01-22 | 广西南宁秀珀生物科技有限公司 | Golden cicada spends artificial culturing method |
| CN109247186A (en) * | 2018-08-29 | 2019-01-22 | 广西南宁秀珀生物科技有限公司 | The method for cultivating golden cicada flower using the olive woods |
| CN109247187A (en) * | 2018-08-29 | 2019-01-22 | 广西南宁秀珀生物科技有限公司 | The high-efficient method for cultivating of golden cicada flower |
| CN109247185A (en) * | 2018-08-29 | 2019-01-22 | 广西南宁秀珀生物科技有限公司 | Imitate the breeding method of wild golden cicada flower |
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Cited By (6)
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| CN109247186A (en) * | 2018-08-29 | 2019-01-22 | 广西南宁秀珀生物科技有限公司 | The method for cultivating golden cicada flower using the olive woods |
| CN109247187A (en) * | 2018-08-29 | 2019-01-22 | 广西南宁秀珀生物科技有限公司 | The high-efficient method for cultivating of golden cicada flower |
| CN109247185A (en) * | 2018-08-29 | 2019-01-22 | 广西南宁秀珀生物科技有限公司 | Imitate the breeding method of wild golden cicada flower |
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Effective date of registration: 20190925 Address after: 221000 Ma Zhuang Village, Pan'an Lake Scenic Area Management Office, Xuzhou City, Jiangsu Province Patentee after: XUZHOU LIANGHAN AGRICULTURE DEVELOPMENT CO., LTD. Address before: Liannan Liancheng County of Fujian province Longyan city 366299 district two building 4, 619 Patentee before: Luo Fu Zhong |
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