CN104755630B - 多阶段核酸扩增 - Google Patents

多阶段核酸扩增 Download PDF

Info

Publication number
CN104755630B
CN104755630B CN201380053605.0A CN201380053605A CN104755630B CN 104755630 B CN104755630 B CN 104755630B CN 201380053605 A CN201380053605 A CN 201380053605A CN 104755630 B CN104755630 B CN 104755630B
Authority
CN
China
Prior art keywords
amplification
stage
nucleic acid
target
sequence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201380053605.0A
Other languages
English (en)
Chinese (zh)
Other versions
CN104755630A (zh
Inventor
N·C·纳尔逊
小莱尔·J·阿诺德
L·戴
S·S·菲尔普斯
J·切利塞里
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Gen Probe Inc
Original Assignee
Gen Probe Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gen Probe Inc filed Critical Gen Probe Inc
Publication of CN104755630A publication Critical patent/CN104755630A/zh
Application granted granted Critical
Publication of CN104755630B publication Critical patent/CN104755630B/zh
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6865—Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Virology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
CN201380053605.0A 2012-08-30 2013-08-30 多阶段核酸扩增 Active CN104755630B (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201261695106P 2012-08-30 2012-08-30
US61/695,106 2012-08-30
US201361846538P 2013-07-15 2013-07-15
US61/846,538 2013-07-15
PCT/US2013/057458 WO2014036369A1 (en) 2012-08-30 2013-08-30 Multiphase nucleic acid amplification

Publications (2)

Publication Number Publication Date
CN104755630A CN104755630A (zh) 2015-07-01
CN104755630B true CN104755630B (zh) 2019-01-11

Family

ID=49162273

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201380053605.0A Active CN104755630B (zh) 2012-08-30 2013-08-30 多阶段核酸扩增

Country Status (9)

Country Link
US (4) US9139870B2 (enExample)
EP (2) EP3640348A1 (enExample)
JP (2) JP6464086B2 (enExample)
CN (1) CN104755630B (enExample)
AU (1) AU2013308647B2 (enExample)
CA (1) CA2883219C (enExample)
DK (1) DK2890814T3 (enExample)
ES (1) ES2761920T3 (enExample)
WO (1) WO2014036369A1 (enExample)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3640348A1 (en) 2012-08-30 2020-04-22 Gen-Probe Incorporated Multiphase nucleic acid amplification
JP7167013B2 (ja) * 2016-10-19 2022-11-08 ジェン－プローブ・インコーポレーテッド Ｃ型肝炎ウイルスを検出または定量するための組成物および方法
CA3042451A1 (en) 2016-11-21 2018-05-24 Gen-Probe Incorporated Compositions and methods for detecting or quantifying hepatitis b virus
WO2019099629A1 (en) 2017-11-17 2019-05-23 Gen-Probe Incorporated COMPOSITIONS AND METHODS FOR DETECTING C1orf43 NUCLEIC ACID
CA3089277A1 (en) 2018-02-06 2019-08-15 Gen-Probe Incorporated Far-red dye probe formulations
AU2019326462B2 (en) * 2018-08-21 2025-11-27 Gen-Probe Incorporated Compositions and methods for amplifying, detecting or quantifying human cytomegalovirus
JP7716995B2 (ja) * 2019-07-03 2025-08-01 ジェン－プローブ・インコーポレーテッド 試料中のＴｒｉｃｈｏｍｏｎａｓ ｖａｇｉｎａｌｉｓの存在を決定する際に使用するためのオリゴヌクレオチド
EP4018004B1 (en) 2019-08-23 2025-06-18 Gen-Probe Incorporated Compositions, methods and kits for detecting treponema pallidum
EP4114985B1 (en) 2020-03-04 2025-05-07 Gen-Probe Incorporated Compositions and methods for detecting sars-cov-2 nucleic acid
WO2024054924A1 (en) 2022-09-08 2024-03-14 Gen-Probe Incorporated Method of detecting nucleic acid analytes using dual-specificity primers

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007146154A1 (en) * 2006-06-06 2007-12-21 Gen-Probe Incorporated Tagged oligonucleotides and their use in nucleic acid amplification methods
WO2010126913A1 (en) * 2009-04-27 2010-11-04 Gen-Probe Incorporated Methods and kits for use in the selective amplification of target sequences
WO2011003020A1 (en) * 2009-07-01 2011-01-06 Gen-Probe Incorporated Methods and compositions for nucleic acid amplification

Family Cites Families (79)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2422956A1 (fr) 1978-04-13 1979-11-09 Pasteur Institut Procede de detection et de caracterisation d'un acide nucleique ou d'une sequence de celui-ci, et reactif enzymatique pour la mise en oeuvre de ce procede
US4786600A (en) 1984-05-25 1988-11-22 The Trustees Of Columbia University In The City Of New York Autocatalytic replication of recombinant RNA
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4868105A (en) 1985-12-11 1989-09-19 Chiron Corporation Solution phase nucleic acid sandwich assay
US4800159A (en) 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
ATE88762T1 (de) 1986-08-11 1993-05-15 Siska Diagnostics Inc Methoden und zusammensetzungen fuer tests mit nukleinsaeuresonden.
US5541308A (en) 1986-11-24 1996-07-30 Gen-Probe Incorporated Nucleic acid probes for detection and/or quantitation of non-viral organisms
IL86724A (en) 1987-06-19 1995-01-24 Siska Diagnostics Inc Methods and kits for amplification and testing of nucleic acid sequences
CA1340843C (en) 1987-07-31 1999-12-07 J. Lawrence Burg Selective amplification of target polynucleotide sequences
US5639604A (en) 1987-09-21 1997-06-17 Gen-Probe Incorporated Homogeneous protection assay
US5283174A (en) 1987-09-21 1994-02-01 Gen-Probe, Incorporated Homogenous protection assay
US5585481A (en) 1987-09-21 1996-12-17 Gen-Probe Incorporated Linking reagents for nucleotide probes
US5124246A (en) 1987-10-15 1992-06-23 Chiron Corporation Nucleic acid multimers and amplified nucleic acid hybridization assays using same
CA1323293C (en) 1987-12-11 1993-10-19 Keith C. Backman Assay using template-dependent nucleic acid probe reorganization
US5130238A (en) 1988-06-24 1992-07-14 Cangene Corporation Enhanced nucleic acid amplification process
US5118801A (en) 1988-09-30 1992-06-02 The Public Health Research Institute Nucleic acid process containing improved molecular switch
US5656207A (en) 1989-06-24 1997-08-12 Gen Probe Incorporated Detecting or quantifying multiple analytes using labelling techniques
CA2020958C (en) 1989-07-11 2005-01-11 Daniel L. Kacian Nucleic acid sequence amplification methods
US5856088A (en) 1989-07-11 1999-01-05 Gen-Probe Incorporated Detection of human immunodeficiency virus type 1
US6589734B1 (en) 1989-07-11 2003-07-08 Gen-Probe Incorporated Detection of HIV
US5104792A (en) 1989-12-21 1992-04-14 The United States Of America As Represented By The Department Of Health And Human Services Method for amplifying unknown nucleic acid sequences
US5516663A (en) 1990-01-26 1996-05-14 Abbott Laboratories Ligase chain reaction with endonuclease IV correction and contamination control
US5849481A (en) 1990-07-27 1998-12-15 Chiron Corporation Nucleic acid hybridization assays employing large comb-type branched polynucleotides
US5378825A (en) 1990-07-27 1995-01-03 Isis Pharmaceuticals, Inc. Backbone modified oligonucleotide analogs
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
DK1695979T3 (da) 1991-12-24 2011-10-10 Isis Pharmaceuticals Inc Gappede modificerede oligonukleotider
US5424413A (en) 1992-01-22 1995-06-13 Gen-Probe Incorporated Branched nucleic acid probes
CA2128891A1 (en) 1992-01-29 1993-08-05 Cylia Keller Polynucleotide immobilized support
CA2135073C (en) 1992-05-06 2002-11-19 Daniel L. Kacian Nucleic acid sequence amplification method, composition and kit
JP4108118B2 (ja) 1993-03-26 2008-06-25 ジェン−プローブ・インコーポレイテッド ヒト・免疫不全ウイルス１型の検出
US5714320A (en) 1993-04-15 1998-02-03 University Of Rochester Rolling circle synthesis of oligonucleotides and amplification of select randomized circular oligonucleotides
US5422252A (en) 1993-06-04 1995-06-06 Becton, Dickinson And Company Simultaneous amplification of multiple targets
US5470723A (en) 1993-05-05 1995-11-28 Becton, Dickinson And Company Detection of mycobacteria by multiplex nucleic acid amplification
CA2167838C (en) 1993-07-23 1999-11-23 Thomas B. Ryder Methods for enhancing nucleic acid amplification
US5925517A (en) 1993-11-12 1999-07-20 The Public Health Research Institute Of The City Of New York, Inc. Detectably labeled dual conformation oligonucleotide probes, assays and kits
US5834252A (en) 1995-04-18 1998-11-10 Glaxo Group Limited End-complementary polymerase reaction
US5547861A (en) 1994-04-18 1996-08-20 Becton, Dickinson And Company Detection of nucleic acid amplification
US6169169B1 (en) 1994-05-19 2001-01-02 Dako A/S PNA probes for detection of Neisseria gonorrhoeae and Chlamydia trachomatis
DE69535240T2 (de) 1994-10-28 2007-06-06 Gen-Probe Inc., San Diego Zusammensetzungen und Verfahren für die gleichzeitige Detektion und Quantifizierung von einer Mehrheit spezifischer Nuklein Säure Sequenzen
US5882856A (en) 1995-06-07 1999-03-16 Genzyme Corporation Universal primer sequence for multiplex DNA amplification
US5981180A (en) 1995-10-11 1999-11-09 Luminex Corporation Multiplexed analysis of clinical specimens apparatus and methods
US5612473A (en) 1996-01-16 1997-03-18 Gull Laboratories Methods, kits and solutions for preparing sample material for nucleic acid amplification
DE69738687D1 (de) 1996-04-12 2008-06-26 Phri Properties Inc Sonden, kits und assays
US5955268A (en) 1996-04-26 1999-09-21 Abbott Laboratories Method and reagent for detecting multiple nucleic acid sequences in a test sample
DK0975807T3 (da) 1997-05-02 2007-01-29 Gen Probe Inc To-trins hybridisering og indfangning af et polynukleotid
US6534273B2 (en) 1997-05-02 2003-03-18 Gen-Probe Incorporated Two-step hybridization and capture of a polynucleotide
US6844158B1 (en) 1997-12-22 2005-01-18 Hitachi Chemical Co., Ltd. Direct RT-PCR on oligonucleotide-immobilized PCR microplates
US6949367B1 (en) 1998-04-03 2005-09-27 Epoch Pharmaceuticals, Inc. Modified oligonucleotides for mismatch discrimination
WO2000001850A2 (en) 1998-07-02 2000-01-13 Gen-Probe Incorporated Molecular torches
US7060813B1 (en) 1999-04-07 2006-06-13 E. I. Du Pont De Nemours And Company Plant RNA-directed RNA polymerase proteins
US6531300B1 (en) 1999-06-02 2003-03-11 Saigene Corporation Target amplification of nucleic acid with mutant RNA polymerase
EP1187939B9 (en) 1999-07-09 2009-08-12 Gen-Probe Incorporated Detection of hiv-1 by nucleic acid amplification
US6828098B2 (en) 2000-05-20 2004-12-07 The Regents Of The University Of Michigan Method of producing a DNA library using positional amplification based on the use of adaptors and nick translation
US7087414B2 (en) 2000-06-06 2006-08-08 Applera Corporation Methods and devices for multiplexing amplification reactions
US6605451B1 (en) 2000-06-06 2003-08-12 Xtrana, Inc. Methods and devices for multiplexing amplification reactions
DE60141087D1 (de) * 2000-06-26 2010-03-04 Nugen Technologies Inc Methoden und zusammensetzungen zur auf transkription basierenden vervielfältigung von nukleinsäuren
US6582920B2 (en) 2000-09-01 2003-06-24 Gen-Probe Incorporated Amplification of HIV-1 RT sequences for detection of sequences associated with drug-resistance mutations
AU2002236524A1 (en) 2000-11-28 2002-06-11 Rosetta Inpharmatics, Inc. In vitro transcription method for rna amplification
WO2002059353A2 (en) 2001-01-26 2002-08-01 Bio S & T Two-step amplification using a program primer followed by specific primers
EP1279736A1 (en) 2001-07-27 2003-01-29 Université de Nantes Methods of RNA and protein synthesis
WO2003044177A2 (en) * 2001-11-19 2003-05-30 Parallele Bioscience, Inc. Multiplex pcr
GB0130955D0 (en) 2001-12-24 2002-02-13 Cancer Res Ventures Expression system
US7135202B2 (en) 2003-07-23 2006-11-14 Hormel Foods, Llc Method of vacuum tumbling for processing meat
EP1508624A1 (en) 2003-08-22 2005-02-23 Institut National De La Sante Et De La Recherche Medicale (Inserm) A quantification method for integrated viruses
US7851148B2 (en) 2003-10-13 2010-12-14 Qiagen Gmbh Method and kit for primer based multiplex amplification of nucleic acids employing primer binding tags
EP2251442B9 (en) 2003-12-19 2012-04-25 Gen-Probe Incorporated Compositions, methods and kits for detecting the nucleic acids of HIV-1 and HIV-2
US20060141518A1 (en) 2004-03-24 2006-06-29 Lao Kai Q Detection of gene expression
JP5020818B2 (ja) 2004-07-01 2012-09-05 ジェン−プローブ・インコーポレーテッド 生物学的試料中の核酸を検出するための方法及び組成物
US7696337B2 (en) * 2004-08-27 2010-04-13 Gen-Probe Incorporated Composition kits and methods for performing amplification reactions
CA2929741A1 (en) 2004-09-30 2006-04-13 Gen-Probe Incorporated Assay for detecting and quantifying hiv-1
EP1853732A2 (en) 2005-02-28 2007-11-14 Gen-Probe Incorporated Compositions and methods of detecting an analyte by using a nucleic acid hybridization switch probe
WO2008016988A1 (en) 2006-08-01 2008-02-07 Gen-Probe Incorporated Methods of nonspecific target capture of nucleic acids
EP1921156A1 (en) 2006-11-10 2008-05-14 bioMerieux B.V. Improved multiplex nucleic acid amplification using blocked primers
AU2007348215A1 (en) * 2007-03-01 2008-09-12 Gen-Probe Incorporated Methods and kits for amplifying DNA
EP2288726B1 (en) * 2008-05-13 2012-06-13 Gen-Probe Incorporated Inactivatable target capture oligomers for use in the selective hybridization and capture of target nucleic acid sequences
US8815515B1 (en) * 2009-09-24 2014-08-26 University Of Utah Research Foundation Methods, compositions, and kits for rare allele detection
EP2607495A1 (en) * 2011-12-23 2013-06-26 Genomica S.A.U. Method for detection of KRAS mutations
EP3640348A1 (en) * 2012-08-30 2020-04-22 Gen-Probe Incorporated Multiphase nucleic acid amplification

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007146154A1 (en) * 2006-06-06 2007-12-21 Gen-Probe Incorporated Tagged oligonucleotides and their use in nucleic acid amplification methods
WO2010126913A1 (en) * 2009-04-27 2010-11-04 Gen-Probe Incorporated Methods and kits for use in the selective amplification of target sequences
WO2011003020A1 (en) * 2009-07-01 2011-01-06 Gen-Probe Incorporated Methods and compositions for nucleic acid amplification

Also Published As

Publication number Publication date
JP2015528294A (ja) 2015-09-28
EP2890814B1 (en) 2019-11-13
US20190185911A1 (en) 2019-06-20
US9139870B2 (en) 2015-09-22
EP2890814A1 (en) 2015-07-08
AU2013308647B2 (en) 2018-10-18
JP2018038424A (ja) 2018-03-15
US12351858B2 (en) 2025-07-08
ES2761920T3 (es) 2020-05-21
US11859238B2 (en) 2024-01-02
CA2883219A1 (en) 2014-03-06
CA2883219C (en) 2020-12-29
US20210403982A1 (en) 2021-12-30
EP3640348A1 (en) 2020-04-22
US10196674B2 (en) 2019-02-05
WO2014036369A1 (en) 2014-03-06
AU2013308647A1 (en) 2015-04-16
HK1211988A1 (en) 2016-06-03
DK2890814T3 (da) 2019-12-16
CN104755630A (zh) 2015-07-01
JP6464086B2 (ja) 2019-02-06
US20160002709A1 (en) 2016-01-07
US20140066326A1 (en) 2014-03-06

Similar Documents

Publication Publication Date Title
CN104755630B (zh) 多阶段核酸扩增
JP6416356B2 (ja) 核酸増幅のための方法および組成物
US20250137034A1 (en) Sample preparation method and system
JP2019509724A (ja) ヌクレアーゼ保護を使用する直接標的シーケンシングの方法
WO2023170144A1 (en) Method of detection of a target nucleic acid sequence
EP4490321A1 (en) Method of detection of a target nucleic acid sequence in a single reaction vessel
HK1211988B (en) Multiphase nucleic acid amplification
HK1219117B (zh) 使用拼装序列扩增片段化的目标核酸的方法

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant