CN104745669A - 用于对单个细胞所具有的细胞毒性进行评价的组合物以及方法 - Google Patents

用于对单个细胞所具有的细胞毒性进行评价的组合物以及方法 Download PDF

Info

Publication number
CN104745669A
CN104745669A CN201510140107.0A CN201510140107A CN104745669A CN 104745669 A CN104745669 A CN 104745669A CN 201510140107 A CN201510140107 A CN 201510140107A CN 104745669 A CN104745669 A CN 104745669A
Authority
CN
China
Prior art keywords
cells
cell
hiv
immunodeficiency virus
human immunodeficiency
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201510140107.0A
Other languages
English (en)
Chinese (zh)
Inventor
J.C.洛夫
N.范阮达若詹
B.居尔格
B.沃克
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
General Hospital Corp
Massachusetts Institute of Technology
Original Assignee
General Hospital Corp
Massachusetts Institute of Technology
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by General Hospital Corp, Massachusetts Institute of Technology filed Critical General Hospital Corp
Publication of CN104745669A publication Critical patent/CN104745669A/zh
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells
    • G01N33/56972—White blood cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G01N33/5047—Cells of the immune system
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/563—Immunoassay; Biospecific binding assay; Materials therefor involving antibody fragments
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B30/00—Methods of screening libraries
    • C40B30/06—Methods of screening libraries by measuring effects on living organisms, tissues or cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/005—Assays involving biological materials from specific organisms or of a specific nature from viruses
    • G01N2333/08—RNA viruses
    • G01N2333/15—Retroviridae, e.g. bovine leukaemia virus, feline leukaemia virus, feline leukaemia virus, human T-cell leukaemia-lymphoma virus
    • G01N2333/155—Lentiviridae, e.g. visna-maedi virus, equine infectious virus, FIV, SIV
    • G01N2333/16—HIV-1, HIV-2

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Cell Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Toxicology (AREA)
  • Virology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
CN201510140107.0A 2009-01-21 2009-07-13 用于对单个细胞所具有的细胞毒性进行评价的组合物以及方法 Pending CN104745669A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US14610609P 2009-01-21 2009-01-21
US61/146106 2009-01-21
CN2009801551526A CN102388145A (zh) 2009-01-21 2009-07-13 用于对单个细胞所具有的细胞毒性进行评价的组合物以及方法

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
CN2009801551526A Division CN102388145A (zh) 2009-01-21 2009-07-13 用于对单个细胞所具有的细胞毒性进行评价的组合物以及方法

Publications (1)

Publication Number Publication Date
CN104745669A true CN104745669A (zh) 2015-07-01

Family

ID=42356144

Family Applications (2)

Application Number Title Priority Date Filing Date
CN201510140107.0A Pending CN104745669A (zh) 2009-01-21 2009-07-13 用于对单个细胞所具有的细胞毒性进行评价的组合物以及方法
CN2009801551526A Pending CN102388145A (zh) 2009-01-21 2009-07-13 用于对单个细胞所具有的细胞毒性进行评价的组合物以及方法

Family Applications After (1)

Application Number Title Priority Date Filing Date
CN2009801551526A Pending CN102388145A (zh) 2009-01-21 2009-07-13 用于对单个细胞所具有的细胞毒性进行评价的组合物以及方法

Country Status (11)

Country Link
US (1) US9244071B2 (https=)
EP (1) EP2389446B1 (https=)
JP (2) JP2012515548A (https=)
KR (1) KR20110134386A (https=)
CN (2) CN104745669A (https=)
AU (1) AU2009338127B2 (https=)
CA (1) CA2689681C (https=)
IL (1) IL213938A0 (https=)
RU (1) RU2532228C2 (https=)
SG (2) SG172889A1 (https=)
WO (1) WO2010085275A1 (https=)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113614532A (zh) * 2019-01-16 2021-11-05 烟台澳斯邦生物工程有限公司 沉积用于显微镜检查的生物样品的自动液体处理系统和方法

Families Citing this family (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9404924B2 (en) 2008-12-04 2016-08-02 Massachusetts Institute Of Technology Method of performing one-step, single cell RT-PCR
EP2370814B1 (en) 2008-12-04 2015-04-29 Massachusetts Institute of Technology Method for diagnosing allergic reactions
US9244071B2 (en) 2009-01-21 2016-01-26 Massachusetts Institute Of Technology Compositions and methods for assessing cytotoxicity of single cells
EP2646830B1 (en) * 2010-12-03 2016-04-13 Cellply S.R.L. Rapid screening of monoclonal antibodies
WO2012072822A1 (en) 2010-12-03 2012-06-07 Mindseeds Laboratories Srl Microanalysis of cellular function
CA2856868C (en) 2011-12-13 2020-07-07 Single Cell Technology, Inc. Method of screening a plurality of single secreting cells for functional activity
US20150191772A1 (en) 2012-06-29 2015-07-09 Danmarks Tekniske Universitet Method of charging a test carrier and a test carrier
CN108267581A (zh) * 2012-08-24 2018-07-10 耶鲁大学 用于高通量多重检测的系统、装置和方法
EP3039162B1 (en) * 2013-08-26 2019-01-02 President and Fellows of Harvard College Determination of immune cells and other cells
PT3099172T (pt) 2014-01-31 2021-11-08 Agbiome Inc Agentes modificados de controlo biológico e seus usos
US9877486B2 (en) 2014-01-31 2018-01-30 AgBiome, Inc. Methods of growing plants using modified biological control agents
CN112782140A (zh) 2014-12-03 2021-05-11 伊索普莱西斯公司 细胞分泌特征的分析和筛选
US10488321B2 (en) 2015-03-19 2019-11-26 The Board Of Trustees Of The Leland Stanford Junior University Devices and methods for high-throughput single cell and biomolecule analysis and retrieval in a microfluidic chip
EP4306955B1 (en) 2015-03-26 2025-05-14 University of Houston System Integrated functional and molecular profiling of cells
WO2017124101A2 (en) 2016-01-15 2017-07-20 The Broad Institute Inc. Semi-permeable arrays for analyzing biological systems and methods of using same
US12345708B2 (en) 2016-03-25 2025-07-01 President And Fellows Of Harvard College Microfluidic determination of immune and other cells
US20190106666A1 (en) * 2016-03-30 2019-04-11 Sony Corporation Cell observation device, method for evaluating activity level of immune cells, and method for controlling quality of immune cells
EP3258240B1 (en) 2016-06-14 2023-06-07 Cellply S.R.L. Screening kit and methods
EP3472351B1 (de) 2016-06-15 2020-10-28 Ludwig-Maximilians-Universität München Einzelmolekülnachweis bzw. -quantifizierung durch dna-nanotechnologie
CN106086171A (zh) * 2016-06-15 2016-11-09 广州中大南沙科技创新产业园有限公司 一种抑制肿瘤细胞增殖的药物筛选方法
EP3510398B1 (en) 2016-09-12 2026-02-25 Isoplexis Corporation System and methods for multiplexed analysis of cellular and other immunotherapeutics
AU2017345402A1 (en) * 2016-10-19 2019-05-23 General Automation Lab Technologies Inc. High resolution systems, kits, apparatus, and methods for screening microorganisms and other high throughput microbiology applications
EP4036578A1 (en) * 2016-11-11 2022-08-03 Isoplexis Corporation Compositions and methods for the simultaneous genomic, transcriptomic and proteomic analysis of single cells
US11085912B2 (en) * 2016-12-14 2021-08-10 University Of Cincinnati Methods of diagnosing Clostridium difficile infection or recurrence in a subject
SE543211C2 (en) * 2017-06-29 2020-10-27 Mabtech Production Ab Method and system for analyzing Fluorospot assays
WO2019113457A1 (en) 2017-12-07 2019-06-13 Massachusetts Institute Of Technology Single cell analyses
CN108728356B (zh) * 2018-05-23 2022-02-22 北京科技大学 用于不同三维细胞团配对的装置及共培养方法
WO2020171020A1 (ja) * 2019-02-18 2020-08-27 株式会社エヌビィー健康研究所 細胞の選抜方法、核酸の製造方法、組換え細胞の製造方法、目的物質の製造方法、医薬組成物の製造方法、及び試薬
US12064768B2 (en) 2020-02-21 2024-08-20 Northeastern University Single cell isolation and processing system with reversible well shape
US20240182849A1 (en) 2022-11-07 2024-06-06 Shennon Biotechnologies Inc. Microfluidic Devices for High Throughput Screening of Cell-Cell Interactions

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL68507A (en) 1982-05-10 1986-01-31 Univ Bar Ilan System and methods for cell selection
SU1650094A1 (ru) * 1987-12-29 1991-05-23 Научно-исследовательский институт проктологии Способ диагностики прот женности активного воспалени в толстой кишке при неспецифическом звенном колите
US6410252B1 (en) 1995-12-22 2002-06-25 Case Western Reserve University Methods for measuring T cell cytokines
US6210910B1 (en) 1998-03-02 2001-04-03 Trustees Of Tufts College Optical fiber biosensor array comprising cell populations confined to microcavities
JP2003501011A (ja) * 1999-04-09 2003-01-14 ヒューマン ジノーム サイエンシーズ， インコーポレイテッド ４９個のヒト分泌タンパク質
JP2003502062A (ja) * 1999-06-11 2003-01-21 ヒューマン ジノーム サイエンシーズ， インコーポレイテッド ４９個のヒト分泌タンパク質
US7332286B2 (en) 2001-02-02 2008-02-19 University Of Pennsylvania Peptide or protein microassay method and apparatus
US20020141906A1 (en) 2001-03-30 2002-10-03 American Type Culture Collection Microtiter plate sealing cover with well identifiers
WO2007035633A2 (en) 2005-09-16 2007-03-29 President & Fellows Of Harvard College Screening assays and methods
DK2297333T3 (en) 2008-05-30 2015-04-07 Massachusetts Inst Technology Method for spatial separation and for screening cells
EP2370814B1 (en) 2008-12-04 2015-04-29 Massachusetts Institute of Technology Method for diagnosing allergic reactions
US9404924B2 (en) 2008-12-04 2016-08-02 Massachusetts Institute Of Technology Method of performing one-step, single cell RT-PCR
US9244071B2 (en) 2009-01-21 2016-01-26 Massachusetts Institute Of Technology Compositions and methods for assessing cytotoxicity of single cells
US8569046B2 (en) 2009-02-20 2013-10-29 Massachusetts Institute Of Technology Microarray with microchannels

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113614532A (zh) * 2019-01-16 2021-11-05 烟台澳斯邦生物工程有限公司 沉积用于显微镜检查的生物样品的自动液体处理系统和方法

Also Published As

Publication number Publication date
KR20110134386A (ko) 2011-12-14
JP2012515548A (ja) 2012-07-12
JP6017620B2 (ja) 2016-11-02
RU2532228C2 (ru) 2014-10-27
IL213938A0 (en) 2011-07-31
CA2689681A1 (en) 2010-07-21
US9244071B2 (en) 2016-01-26
AU2009338127B2 (en) 2015-11-26
CN102388145A (zh) 2012-03-21
SG172889A1 (en) 2011-08-29
AU2009338127A1 (en) 2011-07-28
RU2011134898A (ru) 2013-02-27
US20120149592A1 (en) 2012-06-14
EP2389446A4 (en) 2012-10-03
JP2015144617A (ja) 2015-08-13
WO2010085275A1 (en) 2010-07-29
SG10201401643PA (en) 2014-08-28
EP2389446A1 (en) 2011-11-30
CA2689681C (en) 2017-08-29
EP2389446B1 (en) 2017-07-05

Similar Documents

Publication Publication Date Title
JP6017620B2 (ja) 単一細胞の細胞傷害性を査定するための組成物および方法
CN114616467B (zh) 筛选原代人类细胞中的同源t细胞和表位反应性的高通量方法
US8975069B2 (en) Method for identifying antigen-specific regulatory T cells
JP2015144617A5 (https=)
Heutinck et al. Virus-specific CD8+ T cells cross-reactive to donor-alloantigen are transiently present in the circulation of kidney transplant recipients infected with CMV and/or EBV
Sharma et al. Mucosal-associated invariant T cells develop an innate-like transcriptomic program in anti-mycobacterial responses
WO2019183610A9 (en) Tissue resident memory cell profiles, and uses thereof
WO2016086029A1 (en) Characterization of adaptive immune response to vaccination or infection using immune repertoire sequencing
WO2017144621A1 (en) T cell receptors from the hiv-specific repertoire, means for their production and therapeutic uses thereof
Nyanhete et al. HLA class II-restricted CD8+ T cells in HIV-1 virus controllers
Kocher et al. Vaccination-induced T cell responses maintain polyclonality with high antigen receptor avidity
Mahlobo et al. The impact of HIV infection on the frequencies, function, spatial localization and heterogeneity of T follicular regulatory cells (TFRs) within human lymph nodes
Kocher et al. Quality of vaccination-induced T cell responses is conveyed by polyclonality and high, but not maximum, antigen receptor avidity
Richard Toll-like receptor (TLR) 7 and TLR8 stimulation of mucosal-associated invariant T cells and gamma delta T cells: a role in HIV susceptibility
Meckiff Evolution of the human CD4+ T cell response to Epstein-Barr virus infection-analysis of systemic and local immune responses
Kahia Defining early CD8+ T cell responses during acute and chronic viral infection using single-cell RNA-seq analysis
Lee et al. Robust effector memory features of human T-bethi B cells induced by repeated mRNA vaccination
Phetsouphanh Novel approaches to the characterization of antigen-specific CD4+ T cells in HIV-1 infection
De Simone Dissecting Functional Heterogeneity in the Human CD8+ T Cell Compartment to Characterize Superior Effector Responses
Catry Specific T-and B-cell responses against Human cytomegalovirus after Hematopoietic stem cell transplantation
Wragg et al. High CD26 and Low CD94 Expression Identifies an IL-23 Responsive Vd2 T Cell Subset with a MAIT Cell-like Transcriptional Profile
Clutton HIV-specific interleukin-10 responses and immune modulation
Nguyen Characterization of naïve immune cell subsets important in HIV/SIV pathogenesis as a baseline for RNAseq deconvolution

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
WD01 Invention patent application deemed withdrawn after publication
WD01 Invention patent application deemed withdrawn after publication

Application publication date: 20150701