CN104739968A - Method for extracting total saponins of semen ziziphi spinosae - Google Patents
Method for extracting total saponins of semen ziziphi spinosae Download PDFInfo
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- CN104739968A CN104739968A CN201510134107.XA CN201510134107A CN104739968A CN 104739968 A CN104739968 A CN 104739968A CN 201510134107 A CN201510134107 A CN 201510134107A CN 104739968 A CN104739968 A CN 104739968A
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- ziziphi spinosae
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Abstract
The invention discloses a method for extracting total saponins of semen ziziphi spinosae. The method comprises the following steps: carrying out degreasing treatment on semen ziziphi spinosae powder with petroleum ether; soaking with ethanol; finally, carrying out microwave-assisted extraction twice, filtering, and concentrating to obtain the total saponins of the semen ziziphi spinosae. According to the method for extracting the total saponins of the semen ziziphi spinosae, the semen ziziphi spinosae powder soaked with ethanol is extracted under the help of microwaves so that the extraction rate of the medicinal material is improved; the extraction method is simple and feasible; the extraction time can be effectively shortened; and energy sources are saved.
Description
Technical field
The invention belongs to technical field of extraction of Chinese traditional medicine, be specifically related to a kind of extracting method of Semen Ziziphi Spinosae total saponins.
Background technology
Semen Ziziphi Spinosae is the dry mature seed of Rhamnaceae plant Ziziphi Spinosae, and medicinal history is long, is just classified as top grade in the Shennong's Herbal of Han dynasty.Semen Ziziphi Spinosae has mind calming, calms the nerves, nourishing the liver, arresting sweating effect, be usually used in the treatment of the diseases such as restlessness of asrhenia type and insomnia, palpitation with fear dreaminess, the empty hyperhidrosis of body, Tianjin wound be thirsty, belong to first integration of edible and medicinal herbs dual purpose product that Ministry of Public Health is promulgated.Research shows that Semen Ziziphi Spinosae has obvious regulating action to cardiovascular system immune system of unifying, and has arteriosclerosis, reduces blood pressure, the effect such as adjusting blood lipid, anti-heart rhythm disorders, and toxicity is very little.The primary efficacy of Semen Ziziphi Spinosae is mind tranquilizing and the heart calming, main tonifying five ZANG-organs; wherein Semen Ziziphi Spinosae total saponins is one of main component of Semen Ziziphi Spinosae sedative-hypnotic effect, has suppression central nervous system, anti peroxidation of lipid; resist myocardial ischemia, the pharmacologically actives such as protection ischemic brain injury.
The extractive technique of current Semen Ziziphi Spinosae total saponins mainly contains water bath reflux method, ultrasonic extraction, high speed adverse current chromatogram partition method and Enzymatic Extraction etc., and the major drawbacks of these methods is that gained Semen Ziziphi Spinosae total saponins purity is low, extraction energy consumption is large, organic solvent environmental pollution is serious.
Summary of the invention
The object of this invention is to provide a kind of extracting method of Semen Ziziphi Spinosae total saponins, solve the problem of the large and organic solvent environmental pollution of extraction energy consumption that existing extracting method exists.
The technical solution adopted in the present invention is, a kind of extracting method of Semen Ziziphi Spinosae total saponins, and Spine Date Seed is used soak with ethanol after defat with petroleum ether process, finally uses microwave radiation exaraction twice, filtration, concentrates, obtain Semen Ziziphi Spinosae total saponins.
Feature of the present invention is also,
Spine Date Seed obtains in the following manner: Semen Ziziphi Spinosae is removed impurity, puts into the 37 DEG C of dryings of constant temperature blast drier; Then pulverize with high speed Universalpulverizer and cross 40 mesh sieves, obtaining Spine Date Seed.
Soak with ethanol adopts concentration to be the soak with ethanol 30min of 70%, and liquid ratio is 12 ~ 20:1ml/g.
The technological parameter of microwave radiation exaraction is: power 1000W, and temperature is 40 ~ 60 DEG C, and extraction time is 4 ~ 8min.
The invention has the beneficial effects as follows, the extracting method of Semen Ziziphi Spinosae total saponins of the present invention, adopt microwave-assisted to extract the Spine Date Seed after soak with ethanol, improve the extraction ratio of medical material, extracting method is simple, and can effectively shorten extraction time, energy savings.
Detailed description of the invention
Below in conjunction with detailed description of the invention, the present invention is described in detail.
The extracting method of Semen Ziziphi Spinosae total saponins of the present invention, uses soak with ethanol by Spine Date Seed after defat with petroleum ether process, finally uses microwave radiation exaraction twice, filtration, concentrates, obtain Semen Ziziphi Spinosae total saponins.
Specifically implement according to following steps:
Step 1, removes impurity by Semen Ziziphi Spinosae, puts into the 37 DEG C of dryings of constant temperature blast drier; Then pulverize with high speed Universalpulverizer and cross 40 mesh sieves, obtaining Spine Date Seed;
Step 2, adds petroleum ether in Spine Date Seed, uses cable type extractor according reflux, extract, to extracting liquid colourless;
Step 3, the Spine Date Seed after step 2 being processed adopts concentration to be the soak with ethanol 30min of 70%, and liquid ratio is 12 ~ 20:1ml/g;
Step 4, by the Spine Date Seed after soak with ethanol through microwave radiation exaraction twice, power 1000W, temperature is 40 ~ 60 DEG C, and extraction time is 4 ~ 8min, filters, concentrates, obtain Semen Ziziphi Spinosae total saponins.
The extracting method of Semen Ziziphi Spinosae total saponins of the present invention, adopt microwave-assisted to extract the Spine Date Seed after soak with ethanol, improve the extraction ratio of medical material, extracting method is simple, and can effectively shorten extraction time, energy savings.
Microwave-assisted extraction technique is different from traditional solvent extraction, as a kind of new and effective abstraction technique, is research heat subject in recent years.Microwave can penetrate spe medium, direct heating material, can shorten extraction time and improve extraction efficiency.And in traditional mode of heating, because practical operation needs, chamber wall is made up of the non-conductor of heat mostly, and heat is transmitted to solution inside by wall needs the time; On the contrary, microwave heating is an inner heating process, and it is different from common external heat mode by the transmission of heat ecto-entad, but directly acts on inside and outside medium molecule simultaneously, be that whole material is heated simultaneously, thus ensure that the quick conduction of energy and make full use of.
Embodiment 1
Step 1, removes impurity by Semen Ziziphi Spinosae, puts into the 37 DEG C of dryings of constant temperature blast drier; Then pulverize with high speed Universalpulverizer and cross 40 mesh sieves, obtaining Spine Date Seed;
Step 2, adds petroleum ether in Spine Date Seed, uses cable type extractor according reflux, extract, to extracting liquid colourless;
Step 3, the Spine Date Seed after step 2 being processed adopts concentration to be the soak with ethanol 30min of 70%, and liquid ratio is 20:1ml/g;
Step 4, by the Spine Date Seed after soak with ethanol through microwave radiation exaraction twice, power 1000W, temperature is 50 DEG C, and extraction time is 4min, filters, concentrates, obtain Semen Ziziphi Spinosae total saponins.
Its extraction ratio is 2.622% after testing.
Embodiment 2 ~ 17
See embodiment 1, other conditions are constant, and the relevant parameter of microwave radiation exaraction is as shown in table 1.
Embodiment 2 ~ 17 microwave radiation exaraction relevant parameter and extraction ratio
| Embodiment | Extracting temperature/DEG C | Extraction time/min | Liquid ratio | Extraction ratio/% |
| 2 | 50 | 4 | 12 | 2.598 |
| 3 | 50 | 6 | 16 | 2.642 |
| 4 | 40 | 6 | 20 | 2.584 |
| 5 | 60 | 8 | 16 | 2.608 |
| 6 | 40 | 4 | 16 | 2.580 |
| 7 | 50 | 6 | 20 | 2.604 |
| 8 | 50 | 6 | 16 | 2.642 |
| 9 | 50 | 6 | 16 | 2.638 |
| 10 | 50 | 6 | 16 | 2.636 |
| 11 | 40 | 8 | 16 | 2.586 |
| 12 | 60 | 4 | 16 | 2.634 |
| 13 | 60 | 6 | 12 | 2.617 |
| 14 | 50 | 8 | 12 | 2.598 |
| 15 | 60 | 6 | 20 | 2.632 |
| 16 | 50 | 6 | 16 | 2.634 |
| 17 | 40 | 6 | 12 | 2.582 |
The comparison of Different Extraction Method:
Comparative example 1, reflux extraction:
Step 1, removes impurity by Semen Ziziphi Spinosae, puts into the 37 DEG C of dryings of constant temperature blast drier; Then pulverize with high speed Universalpulverizer and cross 40 mesh sieves, obtaining Spine Date Seed;
Step 2, adds petroleum ether in Spine Date Seed, uses cable type extractor according reflux, extract, to extracting liquid colourless;
Step 3, the Spine Date Seed after step 2 being processed adopts concentration to be the soak with ethanol 30min of 70%, and liquid ratio is 20:1ml/g;
Step 4, by the Spine Date Seed after soak with ethanol through reflux, extract, 3 times, temperature is 61 DEG C, and extraction time is 30min, filters, concentrated, obtains Semen Ziziphi Spinosae total saponins.
Its extraction ratio is 2.35% after testing.
Comparative example 2, ultrasonic extraction:
Step 1, removes impurity by Semen Ziziphi Spinosae, puts into the 37 DEG C of dryings of constant temperature blast drier; Then pulverize with high speed Universalpulverizer and cross 40 mesh sieves, obtaining Spine Date Seed;
Step 2, adds petroleum ether in Spine Date Seed, uses cable type extractor according reflux, extract, to extracting liquid colourless;
Step 3, the Spine Date Seed after step 2 being processed adopts concentration to be the soak with ethanol 30min of 70%, and liquid ratio is 20:1ml/g;
Step 4, by the Spine Date Seed after soak with ethanol through ultrasonic extraction 2 times, temperature is 61 DEG C, and extraction time is 30min, filters, concentrated, obtains Semen Ziziphi Spinosae total saponins.
Its extraction ratio is 2.45% after testing.
Result shows, compares with ultrasonic extracting method with traditional backflow, and the extraction ratio of microwave radiation exaraction is higher, and can effectively shorten extraction time, energy savings.
Claims (4)
1. an extracting method for Semen Ziziphi Spinosae total saponins, is characterized in that, Spine Date Seed is used soak with ethanol after defat with petroleum ether process, finally uses microwave radiation exaraction twice, filters, concentrates, obtain Semen Ziziphi Spinosae total saponins.
2. the extracting method of Semen Ziziphi Spinosae total saponins according to claim 1, is characterized in that, Spine Date Seed obtains in the following manner: Semen Ziziphi Spinosae is removed impurity, puts into the 37 DEG C of dryings of constant temperature blast drier; Then pulverize with high speed Universalpulverizer and cross 40 mesh sieves, obtaining Spine Date Seed.
3. the extracting method of Semen Ziziphi Spinosae total saponins according to claim 1, is characterized in that, soak with ethanol adopts concentration to be the soak with ethanol 30min of 70%, and liquid ratio is 12 ~ 20:1ml/g.
4. the extracting method of the Semen Ziziphi Spinosae total saponins according to claim 1 or 3, is characterized in that, the technological parameter of microwave radiation exaraction is: power 1000W, and temperature is 40 ~ 60 DEG C, and extraction time is 4 ~ 8min.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107412371A (en) * | 2017-09-01 | 2017-12-01 | 安徽省芬格欣生物药业有限公司 | It is a kind of that there is mental-tranquilization, the jujuboside extracting method of tranquilizing soporific function |
| CN109907263A (en) * | 2019-04-11 | 2019-06-21 | 山西中医药大学 | A kind of wild jujube fruit powder and preparation method thereof with antioxidation |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102793767A (en) * | 2012-09-04 | 2012-11-28 | 云南云药科技股份有限公司 | Radix notoginseng pharmaceutical composition for treating insomnia as well as preparation method and application of pharmaceutical composition |
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2015
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102793767A (en) * | 2012-09-04 | 2012-11-28 | 云南云药科技股份有限公司 | Radix notoginseng pharmaceutical composition for treating insomnia as well as preparation method and application of pharmaceutical composition |
Non-Patent Citations (2)
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| 房信胜等: "微波辅助提取酸枣仁皂苷A的工艺优化", 《林产化学与工业》 * |
| 马琦: "酸枣仁抗氧化成分提取及其活性", 《过程工程学报》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107412371A (en) * | 2017-09-01 | 2017-12-01 | 安徽省芬格欣生物药业有限公司 | It is a kind of that there is mental-tranquilization, the jujuboside extracting method of tranquilizing soporific function |
| CN109907263A (en) * | 2019-04-11 | 2019-06-21 | 山西中医药大学 | A kind of wild jujube fruit powder and preparation method thereof with antioxidation |
| CN109907263B (en) * | 2019-04-11 | 2022-04-26 | 山西中医药大学 | Wild jujube fruit powder with antioxidation effect and preparation method thereof |
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Application publication date: 20150701 |