CN104738068B - A kind of Herba Dendrobii tillering agent and its application - Google Patents
A kind of Herba Dendrobii tillering agent and its application Download PDFInfo
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- CN104738068B CN104738068B CN201510075186.1A CN201510075186A CN104738068B CN 104738068 B CN104738068 B CN 104738068B CN 201510075186 A CN201510075186 A CN 201510075186A CN 104738068 B CN104738068 B CN 104738068B
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- herba dendrobii
- tillering
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- water
- tillering agent
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Abstract
The invention discloses a kind of Herba Dendrobii tillering agent, it is made up of 6 benzyl aminoadenines (6 BA), Thidiazuron (TDZ), sodium hydroxide and water, described 6 benzyl aminoadenines, the mass volume ratio of Thidiazuron, sodium hydroxide and water are 4mg: 0.5mg: 1 2mg: 10ml.Present invention also offers a kind of application process in terms of promoting Herba Dendrobii tiller and growth for Herba Dendrobii tillering agent, this application process is：From the Herba Dendrobii Seedling of 0.5 1 years, described Herba Dendrobii tillering agent is sprayed using the processing method that root is applied, drenched dendrobium officinale culture medium with water after 24h, terminate the continuous action of tillering agent.Using tillering agent and its application process of the present invention, tillering ability and the speed of growth of Herba Dendrobii being significantly improved, thus promoting Herba Dendrobii division propagation efficiency, improving Herba Dendrobii yield.
Description
Technical field
The present invention relates to a kind of agriculture and forestry technical field of cultivation and in particular to a kind of Herba Dendrobii tillering agent and its promote ferrum
Skin Herba Dendrobii tiller, growth application process.
Background technology
Herba Dendrobii (Dendrobium officinale) is orchid family (Orchidaceae) Dendrobium (Dendrobium)
Plant, also known as Herba hedyotis costatae, are the traditional rare Chinese medicines of China." Taoist Scriptures " is listed in first of " Chinese nine big Herba mesonae chinensis ".Ferrum
Skin Herba Dendrobii is Herba Dendrobii unique Herba Dendrobii plant origin, is embodied in 2010 editions《Pharmacopoeia of People's Republic of China》, mainly effectively
Composition is Herba Dendrobii class polysaccharide, alkaloid, phenols, aminoacid etc., has antitumor, defying age, enhancing human body immunity power, blood sugar lowering
Mitigate the effect such as hepatic injury with effective.
Although Herba Dendrobii can realize seedling by tissue culture method producing in a large number, artificial culture also succeeds,
There is poor growth, yield poorly, artificial culture Herba Dendrobii still cannot meet the market demand, one of reason is iron sheet
Herba Dendrobii nature tillering ability is poor, and natural division propagation efficiency is low, and the yield per unit area after candidum tissue culturing seedling plantation is limited.
Generally require after realizing the tiller of Herba Dendrobii under lab, then be transplanted in natural environment, carry out nature cultivation.Laboratory
Herba Dendrobii tiller needs tissue culture environment and condition, has high demands and wastes time and energy with respect to Herba Dendrobii nature tiller.Accordingly, it would be desirable to set up ferrum
The technology of skin Herba Dendrobii nature tiller, also needs to improve the effective tillering of Herba Dendrobii simultaneously.Herba Dendrobii tiller includes effective tillering
And ineffective tillering, effective tillering refers to the tiller bud that the axillary bud sprouting at place near the ground for the Herba Dendrobii stem is formed, effective tillering
Bud forms a new plant by nature division propagation, can improve the root yield in unit area；Ineffective tillering refers to ferrum
Skin Herba Dendrobii stem middle-end or the axillary bud sprouting on top, also referred to as high-order bud, ineffective tillering can consume maternal nutrient, reduces maternal
Medical value and the speed of growth, destroy Herba Dendrobii medical material commodity.
Content of the invention
For overcoming above-mentioned technological deficiency, the invention provides a kind of Herba Dendrobii tillering agent and its application process.
A kind of Herba Dendrobii tillering agent of the present invention, by 6- benzyl aminoadenine (6-BA), Thidiazuron (TDZ), hydroxide
Sodium and water composition, described 6- benzyl aminoadenine, the mass volume ratio of Thidiazuron, sodium hydroxide and water are 4mg: 0.5mg: 1-
2mg∶10ml.
Preferably, described tillering agent presses following group assignment system：6- benzyl aminoadenine：400mg；Thidiazuron：50mg；Hydrogen-oxygen
Change sodium：100-200mg；Water：1000ml；Fully mix standby.
Present invention also offers a kind of application side in terms of promoting Herba Dendrobii tiller and growth for Herba Dendrobii tillering agent
Method, this application process is：The processing method applied using root, sprays described tillering agent to described Herba Dendrobii, is drenched with water after 24h
Dendrobium officinale culture medium, terminates the continuous action of tillering agent.
Described Herba Dendrobii is the Herba Dendrobii Seedling of plantation 0.5-1, and the cultivation matrix of described Herba Dendrobii is bark, plane
Flower and the mixture of sawdust.
Herba Dendrobii is a kind of preciousness Chinese herbal medicine, and while improving tillering ability, the present invention considers Herba Dendrobii
Medical value and commodity, ineffective tillering is that occurred in the form of forming high-order bud, not only reduces the medical value of female parent, and
And destroy the commodity of Herba Dendrobii medical material；Using tillering agent and the using method of the present invention, do not destroying medical value
Effective tillering ability and the speed of growth of Herba Dendrobii while with commodity, can be significantly improved, promote the plant division of Herba Dendrobii
Reproductive efficiency, improves the yield in Herba Dendrobii unit area.
Brief description
Fig. 1 is that in tillering agent, 6-BA is 800mg L-1When tiller design sketch；
Fig. 2 is that in tillering agent, 6-BA is 400mg L-1When tiller design sketch.
Specific embodiment
For making the present invention easier to understand, with reference to specific embodiment, the present invention is expanded on further.It should be understood that this
A little embodiments are only illustrative of the invention and is not intended to limit the scope of the invention.
Embodiment 1：The tillering ability that the Herba Dendrobii of different growth seedling ages is applied after tillering agent compares
Tillering agent：6-BA 200mg, sodium hydroxide 100mg, water 1000ml, mix homogeneously.
Application process：Root is applied, and described tillering agent is directly acted on Herba Dendrobii root.
Material：Select the Herba Dendrobii of three kinds of different seedling ages, respectively：0.5-1 gives birth to Seedling, 1-2 gives birth to Seedling, 2-3 life
Seedling, the Herba Dendrobii of every kind of seedling age is each to process 18 plants, and each process is repeated 3 times.
Data statisticss：The tiller bud number of each process is counted after 50 days.
When tillering agent is processed 15 days about, all have at the Herba Dendrobii plant basal internode of different seedling ages and expand phenomenon and go out
Existing, the tiller bud of 50 days visible 1-3cm length, the tiller bud number of different seedling age Herba Dendrobii is different.Table 1 is different seedling age
The tillering ability of Herba Dendrobii compares, and with the increase of seedling age, Herba Dendrobii tillering ability is gradually reduced, the raw ferrum of 0.5-1
The tiller bud number that skin Herba Dendrobii is formed is up to 1.71, and 2-3 life only has 0.66.In actual production, 0.5-1 gives birth to
Herba Dendrobii on the occasion of the growth busy season, by the process of tillering agent, promote tillering ability, yield can be effectively improved, also can simultaneously
There is provided more materials for division propagation method, and the raw Herba Dendrobii of 2-3 is close to the harvesting period, the tiller bud of formation is big
It mostly is ineffective tillering bud, i.e. high-order bud, ineffective tillering can consume the nutrition of female parent, reduce the medical value of female parent, and broken
It is broken the commodity of Herba Dendrobii medical material.Therefore, the present invention selects the Herba Dendrobii that planting time is 0.5-1.
Table 1
Embodiment 2：Select the different impacts to tillering ability for the tillering agent application process
From the raw Herba Dendrobii of 0.5-1.
Tillering agent：6-BA 200mg, sodium hydroxide 100mg, water 1000ml, mix homogeneously.
Application process：Root is divided to apply and 2 kinds of process of foliar spray, every kind of method processes 18 plants of Herba Dendrobii, and each processes repetition 3
Secondary.Herba Dendrobii is the raw Herba Dendrobii of 0.5-1.
Data statisticss：The tiller bud number of each process is counted after 50 days.
Table 2 is the different impact contrast table to Herba Dendrobii tiller for the spraying method, and as shown in Table 2, tillering agent passes through foliar spray
When method processes 0.5-1 raw Herba Dendrobii, tiller bud number is only 0.3, and the tiller bud number that root applying method is formed is 1.71.
As can be seen here, root is applied tillering agent and is more beneficial for stimulating the formation of tiller bud.Therefore, in the present invention, the using method of tillering agent selects
The mode that root is applied.
Table 2
Embodiment 3：The optimization that tillering agent is prepared
Material：The raw Herba Dendrobii of 0.5-1.
Application process：Root is applied.
Tillering agent 1：6-BA selects 0,50mg, 200mg, 400mg, 800mg, and sodium hydroxide 100mg, water 1000ml mixing is all
Even, every kind of application process processes 18 plants of Herba Dendrobii, and each process is repeated 3 times.
Tillering agent 2：From the optimal 6-BA mass filtering out in tillering agent 1, add quality be 0,25,50,75,100mg
TDZ, all mix homogeneously with sodium hydroxide 100mg, water 1000ml, 18 plants of every kind of disposal methods Herba Dendrobii, at each
Reason is repeated 3 times.
Data statisticss：Tiller bud number and the plant strain growth length of each process is counted after 50 days
Table 3 be variable concentrations 6-BA to Herba Dendrobii tiller and growth impact contrast table, data display, 6-BA be promote
Enter Herba Dendrobii tiller, the main factor of growth, when being processed with the tillering agent without 6-BA, Herba Dendrobii does not form tiller
Bud；With the increase of 6-BA concentration in tillering agent, tiller bud number is in rising trend, and concentration is 800mg L-1When, tiller bud number
Up to 3.85, but actual observation finds, now Herba Dendrobii defines high-order bud in a large number in stem upper-end part of driving, that is, invalid point
Tiller bud, is shown in that Fig. 1, Fig. 1 are that in tillering agent, 6-BA is 800mg L-1When tiller design sketch, thus L containing 800mg-16-BA's
Tillering agent should not use；6-BA concentration is 400mg L-1When, tiller bud number more a height of 2.33, and now no high-order bud is formed,
See that Fig. 2, Fig. 2 are that in tillering agent, 6-BA is 400mg L-1When tiller design sketch.Secondly, the growth of plant is also subject to difference dense
Degree 6-BA impact, plant strain growth length with 6-BA concentration increase be in first rise after downward trend, 6-BA be 400mg L-1
When, the plant strain growth length of 50 days is 0.2969cm to the maximum.Therefore select L containing 400mg-1The tillering agent of 6-BA can effectively facilitate
The tiller of Herba Dendrobii and growth.
Table 3
Add the TDZ of variable concentrations in the tillering agent 1 containing optium concentration 6-BA, equal to Herba Dendrobii tiller and growth
There is facilitation, the results are shown in Table 4, tiller bud number is in rising trend with the increase of TDZ concentration, add low concentration TDZ (0-
50mg·L-1) tillering agent Herba Dendrobii can be promoted to form effective tiller bud, when TDZ concentration be 50mg L-1When, average effective
Tiller bud number is 3.02, and adds TDZ (the 75-100mg L of high concentration-1) Herba Dendrobii can be made to form high-order bud, unfavorable
In growth；Secondly, add 50mg L-1TDZ tillering agent process Herba Dendrobii when, plant strain growth length reaches within 50 days
0.3128cm, effect is significant.
Therefore, from 400mg 6- benzyl aminoadenine, 50mg Thidiazuron, 100-200mg sodium hydroxide and 1000ml
When the tillering agent root that water is configured to applies process 0.5-1 raw Herba Dendrobii Seedling, promote effective tillering and the best results of growth.
Table 4
Last should be noted that above example is only in order to illustrate technical scheme rather than to present invention guarantor
The restriction of shield scope, although being explained in detail to the present invention with reference to preferred embodiment, those of ordinary skill in the art should
Understand, technical scheme can be modified or equivalent, without deviating from the essence of technical solution of the present invention
And scope.
Claims (3)
1. a kind of Herba Dendrobii tillering agent is it is characterised in that by 6- benzyl aminoadenine (6-BA), Thidiazuron (TDZ), hydroxide
Sodium and water composition, described 6- benzyl aminoadenine, the mass volume ratio of Thidiazuron, sodium hydroxide and water are respectively 4mg: 0.5mg:
1-2mg∶10ml；
Described Herba Dendrobii is the Herba Dendrobii Seedling of plantation 0.5-1, and the cultivation matrix that described Herba Dendrobii adopts is bark, plane
Flower and the mixture of sawdust.
2. Herba Dendrobii tillering agent according to claim 1 is it is characterised in that described tillering agent presses following group assignment system：
6- benzyl aminoadenine：400mg；
Thidiazuron：50mg；
Sodium hydroxide：100-200mg；
Water：1000ml；
Fully mix standby.
3. application process in terms of promoting Herba Dendrobii tiller and growth for the Herba Dendrobii tillering agent described in claim 1, its
It is characterised by that the processing method applied using root sprays described tillering agent to described Herba Dendrobii, drenched iron sheet stone with water after 24h
Dry measure used in former times cultivation matrix.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510075186.1A CN104738068B (en) | 2015-02-12 | 2015-02-12 | A kind of Herba Dendrobii tillering agent and its application |
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