CN104738068A - Dendrobium officinale tillering agent and application thereof - Google Patents
Dendrobium officinale tillering agent and application thereof Download PDFInfo
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- CN104738068A CN104738068A CN201510075186.1A CN201510075186A CN104738068A CN 104738068 A CN104738068 A CN 104738068A CN 201510075186 A CN201510075186 A CN 201510075186A CN 104738068 A CN104738068 A CN 104738068A
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Abstract
The invention discloses a dendrobium officinale tillering agent. The dendrobium officinale tillering agent is prepared from 6-benzylamino adenine (6-BA), thidiazuron (TDZ), sodium hydroxide and water, of which the mass-volume ratio is 4mg:0.5mg:1-2mg:10ml. The invention also provides an application method of the dendrobium officinale tillering agent in the aspects of promoting dendrobium officinale to be tillered and to grow. The application method comprises the steps of selecting a dendrobium officinale seedling growing for 0.5-1 years, spraying the dendrobium officinale tillering agent by a root application method, drenching dendrobium officinale culture medium with water after 24h, and terminating the sustained action of the tillering agent. According to the tillering agent and the application method of the tillering agent, the tillering ability and the growth speed of the dendrobium officinale are improved remarkably, the division propagation efficiency of the dendrobium officinale is increased and the dendrobium officinale yield is improved.
Description
Technical field
The present invention relates to a kind of agriculture and forestry technical field of cultivation, be specifically related to a kind of dendrobium candidum tillering agent and promote that dendrobium candidum is tillered, grown application method.
Background technology
Dendrobium candidum (Dendrobium officinale) is the orchid family (Orchidaceae) Dendrobium (Dendrobium) plant, has another name called ribbed hedyotis herb, is the traditional rare traditional Chinese medicine of China.Be listed in " Taoist Scriptures " first of " Chinese nine immortal grass ".Dendrobium candidum is the unique stem of noble dendrobium plant origin of Tiepi Fengdou, include in 2010 editions Pharmacopoeias of the People's Republic of China, principle active component is stem of noble dendrobium class polysaccharide, alkaloid, phenols, amino acid etc., have antitumor, anti-ageing, strengthen immunity of organisms, hypoglycemic and effectively alleviate the effects such as hepatic injury.
Produce in a large number although dendrobium candidum can realize seedling by tissue culture method, artificial cultivation also succeeds, but the problem such as there is poor growth, yield poorly, artificial cultivation dendrobium candidum still cannot be met the need of market, one of reason is that dendrobium candidum nature tillering ability is poor, nature division propagation efficiency is low, and the yield per unit area after candidum tissue culturing seedling plantation is limited.After generally needing under lab to realize the tillering of dendrobium candidum, then be transplanted in natural environment, carry out nature cultivation.The laboratory stem of noble dendrobium is tillered needs group training environment and condition, high and waste time and energy relative to stem of noble dendrobium requirement of naturally tillering.Therefore, need to set up the technology that dendrobium candidum is tillered naturally, also need the effective tiller improving dendrobium candidum simultaneously.Dendrobium candidum is tillered and is comprised effective tiller and ineffective tillering, effective tiller refers to the tiller bud that the axillary bud sprouting of dendrobium candidum stem at place near the ground is formed, effective tiller bud forms a new plant by nature division propagation, can improve the root yield in unit are; Ineffective tillering refers to the axillary bud sprouting on dendrobium candidum stem middle-end or top, is also referred to as high-order bud, and ineffective tillering can consume maternal nutrient, reduces maternal medical value and growth rate, destroys the commodity of dendrobium candidum medicinal material.
Summary of the invention
For overcoming above-mentioned technological deficiency, the invention provides a kind of dendrobium candidum tillering agent and application method thereof.
A kind of dendrobium candidum tillering agent of the present invention, be made up of 6-benzyl aminoadenine (6-BA), Thidiazuron (TDZ), sodium hydroxide and water, the mass volume ratio of described 6-benzyl aminoadenine, Thidiazuron, sodium hydroxide and water is 4mg: 0.5mg: 1-2mg: 10ml.
Preferably, described tillering agent is by following component preparation: 6-benzyl aminoadenine: 400mg; Thidiazuron: 50mg; Sodium hydroxide: 100-200mg; Water: 1000ml; Abundant mixing is for subsequent use.
Present invention also offers a kind of dendrobium candidum tillering agent at the application method promoting dendrobium candidum to tiller and in growth, this application method is: adopt the processing method that root is executed, described tillering agent is sprayed to described dendrobium candidum, to drench dendrobium officinale culture medium with water after 24h, stop the continuous action of tillering agent.
Described dendrobium candidum is the dendrobium candidum seedling of plantation 0.5-1, and the cultivation matrix of described dendrobium candidum is the mixture of bark, wood shavings and sawdust.
Dendrobium candidum is a kind of precious Chinese herbal medicine, while raising tillering ability, contemplated by the invention medical value and the commodity of dendrobium candidum, ineffective tillering is that the form forming high-order bud occurs, not only reduce maternal medical value, and destroy the commodity of dendrobium candidum medicinal material; Utilize tillering agent of the present invention and using method, while not destroying medical value and commodity, effective tiller ability and the growth rate of dendrobium candidum can be significantly improved, promote the division propagation efficiency of dendrobium candidum, improve the output in dendrobium candidum unit are.
Accompanying drawing explanation
Fig. 1 is that in tillering agent, 6-BA is 800mgL
-1time design sketch of tillering;
Fig. 2 is that in tillering agent, 6-BA is 400mgL
-1time design sketch of tillering.
Embodiment
For making the present invention easier to understand, below in conjunction with specific embodiment, set forth the present invention further.Should be understood that these embodiments are only not used in for illustration of the present invention to limit the scope of the invention.
Embodiment 1: the tillering ability after tillering agent is used to the dendrobium candidum of difference growth seedling age and compares
Tillering agent: 6-BA 200mg, sodium hydroxide 100mg, water 1000ml, mixes.
Application process: root is executed, directly acts on dendrobium candidum root by described tillering agent.
The raw seedling of material: the dendrobium candidum selecting three kinds of different seedling ages, is respectively: 0.5-1, the raw seedling of 1-2, the raw seedling of 2-3, often the dendrobium candidum of kind of seedling age processes 18 strains, and each process repeats 3 times.
Data statistics: the tiller bud number adding up each process for 50 days afterwards.
During tillering agent process about 15 days, the dendrobium candidum plant basal internode place of different seedling age all has expands phenomenon and occurs, the tiller bud that 50 days visible 1-3cm grow, and the tiller bud number of different seedling age dendrobium candidum is different.The tillering ability that table 1 is different seedling age dendrobium candidum compares, and along with the increase of seedling age, dendrobium candidum tillering ability declines gradually, and the tiller bud number that the dendrobium candidum that 0.5-1 is raw is formed can reach 1.71, and 2-3 life only has 0.66.In actual production, the dendrobium candidum of 0.5-1 life, on the occasion of the growth busy season, by the process of tillering agent, promotes tillering ability, effectively can improve output, also can provide more material for division propagation method, and the dendrobium candidum of 2-3 life is close to gathering the period, the tiller bud of formation is mostly ineffective tillering bud simultaneously, i.e. high-order bud, ineffective tillering can consume maternal nutrition, reduces maternal medical value, and destroys the commodity of dendrobium candidum medicinal material.Therefore, the present invention selects planting time to be the dendrobium candidum of 0.5-1.
Table 1
Embodiment 2: select different tillering agent application process on the impact of tillering ability
Select the dendrobium candidum that 0.5-1 is raw.
Tillering agent: 6-BA 200mg, sodium hydroxide 100mg, water 1000ml, mixes.
Application process: a point root is executed and foliar spray 2 kinds process, and the strain of often kind of method process dendrobium candidum 18, each process repeats 3 times.Dendrobium candidum is the dendrobium candidum that 0.5-1 is raw.
Data statistics: the tiller bud number adding up each process for 50 days afterwards.
What table 2 was tillered on dendrobium candidum for different spraying method affects contrast table, and as shown in Table 2, when tillering agent passes through the dendrobium candidum of foliar spray method process 0.5-1 life, tiller bud number is only 0.3, and the tiller bud number that root applying method is formed is 1.71.As can be seen here, root executes the formation that tillering agent is more conducive to stimulating tiller bud.Therefore, the mode that in the present invention, the using method of tillering agent selects root to execute.
Table 2
Embodiment 3: the optimization of tillering agent preparation
The dendrobium candidum that material: 0.5-1 is raw.
Application process: root is executed.
Tillering agent 1:6-BA selection 0,50mg, 200mg, 400mg, 800mg, sodium hydroxide 100mg, water 1000ml mix, and the strain of often kind of application process process dendrobium candidum 18, each process repeats 3 times.
Tillering agent 2: select the best 6-BA quality filtered out in tillering agent 1, add quality be 0,25,50,75, the TDZ of 100mg, all mix with sodium hydroxide 100mg, water 1000ml, the strain of often kind of disposal methods dendrobium candidum 18, each process repeats 3 times.
Data statistics: tiller bud number and the plant strain growth length of adding up each process for 50 days afterwards
Table 3 be the 6-BA of variable concentrations dendrobium candidum is tillered and grow affect contrast table, data show, and 6-BA promotes the dendrobium candidum main factor of tillering, grow, and when use does not add the tillering agent process of 6-BA, dendrobium candidum does not form tiller bud; Along with the increase of 6-BA concentration in tillering agent, tiller bud number is in rising trend, and concentration is 800mgL
-1time, tiller bud number can reach 3.85, but actual observation finds, now dendrobium candidum defines a large amount of high-order bud at stem upper-end part of driving, and namely ineffective tillering bud, is shown in Fig. 1, and Fig. 1 is that in tillering agent, 6-BA is 800mgL
-1time design sketch of tillering, thus containing 800mgL
-1the tillering agent of 6-BA should not use; 6-BA concentration is 400mgL
-1time, tiller bud number is higher is 2.33, and now without high-order Buds formation, sees Fig. 2, and Fig. 2 is that in tillering agent, 6-BA is 400mgL
-1time design sketch of tillering.Secondly, the growth of plant is also subject to the 6-BA impact of variable concentrations, and plant strain growth length is the rear downward trend that first rises with the increase of 6-BA concentration, and 6-BA is 400mgL
-1time, the plant strain growth length of 50 days is 0.2969cm to the maximum.Therefore select containing 400mgL
-1the tillering agent of 6-BA effectively can promote tillering and growing of dendrobium candidum.
Table 3
In the tillering agent 1 containing optium concentration 6-BA, add the TDZ of variable concentrations, tillering to dendrobium candidum and grow all has facilitation, and the results are shown in Table 4, tiller bud number is in rising trend with the increase of TDZ concentration, adds low concentration TDZ (0-50mgL
-1) tillering agent can promote that dendrobium candidum forms effective tiller bud, when TDZ concentration is 50mgL
-1time, average effective tiller bud number is 3.02, and adds the TDZ (75-100mgL of high concentration
-1) dendrobium candidum can be made to form high-order bud, be unfavorable for growth; Secondly, 50mgL is added
-1the tillering agent process dendrobium candidum of TDZ time, within 50 days, plant strain growth length reaches 0.3128cm, Be very effective.
Therefore, when the tillering agent root selecting 400mg 6-benzyl aminoadenine, 50mg Thidiazuron, 100-200mg sodium hydroxide and 1000ml water to be mixed with executes process 0.5-1 life dendrobium candidum seedling, the best results of effective tiller and growth is promoted.
Table 4
Finally to should be noted that; above embodiment is only in order to illustrate technical scheme of the present invention but not limiting the scope of the invention; although be explained in detail the present invention with reference to preferred embodiment; those of ordinary skill in the art is to be understood that; can modify to technical scheme of the present invention or equivalent replacement, and not depart from essence and the scope of technical solution of the present invention.
Claims (4)
1. a dendrobium candidum tillering agent, it is characterized in that, be made up of 6-benzyl aminoadenine (6-BA), Thidiazuron (TDZ), sodium hydroxide and water, the mass volume ratio of described 6-benzyl aminoadenine, Thidiazuron, sodium hydroxide and water is respectively 4mg: 0.5mg: 1-2mg: 10ml.
2. dendrobium candidum tillering agent according to claim 1, is characterized in that, described tillering agent is by following component preparation:
6-benzyl aminoadenine: 400mg;
Thidiazuron: 50mg;
Sodium hydroxide: 100-200mg;
Water: 1000ml;
Abundant mixing is for subsequent use.
3. dendrobium candidum tillering agent according to claim 1 is at the application method promoting dendrobium candidum to tiller and in growth, it is characterized in that, adopt the processing method executed of root, spray described tillering agent to described dendrobium candidum, to drench dendrobium officinale culture medium with water after 24h.
4. application according to claim 3, is characterized in that, described dendrobium candidum is the dendrobium candidum seedling of plantation 0.5-1, and the cultivation matrix of described dendrobium candidum is the mixture of bark, wood shavings and sawdust.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106171995A (en) * | 2016-07-20 | 2016-12-07 | 徐伟明 | A kind of culture medium increasing Herba Dendrobii tillering ability |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH08107730A (en) * | 1994-10-12 | 1996-04-30 | Aisin Seiki Co Ltd | Proliferation of paphiopedilum |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH08107730A (en) * | 1994-10-12 | 1996-04-30 | Aisin Seiki Co Ltd | Proliferation of paphiopedilum |
Non-Patent Citations (2)
| Title |
|---|
| 关杰敏: "铁皮石斛良种繁育与GAP种植关键技术研究", 《广州中医药大学博士毕业论文》 * |
| 金银兵: "6-BA在促进铁皮石斛侧芽萌发中的应用", 《中国农学通报》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106171995A (en) * | 2016-07-20 | 2016-12-07 | 徐伟明 | A kind of culture medium increasing Herba Dendrobii tillering ability |
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