CN104719163B - A kind of method for improving blueberry tissue culture test tube seedling seedling exercising planting percent - Google Patents
A kind of method for improving blueberry tissue culture test tube seedling seedling exercising planting percent Download PDFInfo
- Publication number
- CN104719163B CN104719163B CN201510133364.1A CN201510133364A CN104719163B CN 104719163 B CN104719163 B CN 104719163B CN 201510133364 A CN201510133364 A CN 201510133364A CN 104719163 B CN104719163 B CN 104719163B
- Authority
- CN
- China
- Prior art keywords
- seedling
- culture
- root
- taking root
- miniature
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 235000003095 Vaccinium corymbosum Nutrition 0.000 title claims abstract description 54
- 235000017537 Vaccinium myrtillus Nutrition 0.000 title claims abstract description 54
- 235000021014 blueberries Nutrition 0.000 title claims abstract description 54
- 240000000851 Vaccinium corymbosum Species 0.000 title claims abstract description 51
- 238000000034 method Methods 0.000 title claims abstract description 32
- 238000012360 testing method Methods 0.000 title claims abstract description 17
- 239000001963 growth medium Substances 0.000 claims abstract description 21
- 239000002609 medium Substances 0.000 claims abstract description 8
- 235000019362 perlite Nutrition 0.000 claims abstract description 8
- 239000010451 perlite Substances 0.000 claims abstract description 7
- 239000000243 solution Substances 0.000 claims description 37
- 241000196324 Embryophyta Species 0.000 claims description 21
- 239000005556 hormone Substances 0.000 claims description 12
- 229940088597 hormone Drugs 0.000 claims description 12
- 239000011159 matrix material Substances 0.000 claims description 11
- 235000016709 nutrition Nutrition 0.000 claims description 11
- 230000001954 sterilising effect Effects 0.000 claims description 10
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 9
- 239000004033 plastic Substances 0.000 claims description 8
- 229920003023 plastic Polymers 0.000 claims description 8
- 238000004659 sterilization and disinfection Methods 0.000 claims description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 6
- 150000001875 compounds Chemical class 0.000 claims description 5
- 238000010790 dilution Methods 0.000 claims description 5
- 239000012895 dilution Substances 0.000 claims description 5
- 230000007613 environmental effect Effects 0.000 claims description 5
- 238000005286 illumination Methods 0.000 claims description 5
- 238000011081 inoculation Methods 0.000 claims description 5
- 239000002689 soil Substances 0.000 claims description 5
- 238000012546 transfer Methods 0.000 claims description 5
- 238000002360 preparation method Methods 0.000 claims description 4
- 239000003755 preservative agent Substances 0.000 claims description 4
- 230000002335 preservative effect Effects 0.000 claims description 4
- 239000007921 spray Substances 0.000 claims description 4
- 239000012879 subculture medium Substances 0.000 claims description 4
- 238000012549 training Methods 0.000 claims description 4
- 240000007594 Oryza sativa Species 0.000 claims description 3
- 235000007164 Oryza sativa Nutrition 0.000 claims description 3
- 238000013270 controlled release Methods 0.000 claims description 3
- 239000003337 fertilizer Substances 0.000 claims description 3
- 239000003415 peat Substances 0.000 claims description 3
- 238000004080 punching Methods 0.000 claims description 3
- 235000009566 rice Nutrition 0.000 claims description 3
- 238000002791 soaking Methods 0.000 claims description 3
- 239000000758 substrate Substances 0.000 claims description 3
- 238000003973 irrigation Methods 0.000 claims description 2
- 230000002262 irrigation Effects 0.000 claims description 2
- 230000003020 moisturizing effect Effects 0.000 claims description 2
- 230000008569 process Effects 0.000 claims description 2
- 238000007789 sealing Methods 0.000 claims description 2
- 239000000126 substance Substances 0.000 claims description 2
- 238000001816 cooling Methods 0.000 claims 1
- 238000002156 mixing Methods 0.000 claims 1
- 229910001562 pearlite Inorganic materials 0.000 claims 1
- 238000005516 engineering process Methods 0.000 abstract description 5
- 239000000463 material Substances 0.000 abstract description 5
- 238000013459 approach Methods 0.000 abstract description 2
- 230000008859 change Effects 0.000 abstract description 2
- 238000001000 micrograph Methods 0.000 description 5
- 238000009395 breeding Methods 0.000 description 4
- 230000001488 breeding effect Effects 0.000 description 4
- 244000077233 Vaccinium uliginosum Species 0.000 description 3
- 230000002378 acidificating effect Effects 0.000 description 3
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 238000009835 boiling Methods 0.000 description 2
- 238000005520 cutting process Methods 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 239000010903 husk Substances 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 230000000050 nutritive effect Effects 0.000 description 2
- 239000006259 organic additive Substances 0.000 description 2
- 230000004083 survival effect Effects 0.000 description 2
- 230000007704 transition Effects 0.000 description 2
- 244000283207 Indigofera tinctoria Species 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 150000007513 acids Chemical class 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 230000003064 anti-oxidating effect Effects 0.000 description 1
- 238000010923 batch production Methods 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 235000021028 berry Nutrition 0.000 description 1
- 235000015165 citric acid Nutrition 0.000 description 1
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid group Chemical class C(CC(O)(C(=O)O)CC(=O)O)(=O)O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 1
- 238000013329 compounding Methods 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000007598 dipping method Methods 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 239000010931 gold Substances 0.000 description 1
- 229910052737 gold Inorganic materials 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 238000007654 immersion Methods 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 239000012499 inoculation medium Substances 0.000 description 1
- 238000002386 leaching Methods 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 239000002777 nucleoside Substances 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 210000001747 pupil Anatomy 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
- 239000011435 rock Substances 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
- UZKQTCBAMSWPJD-UQCOIBPSSA-N trans-Zeatin Natural products OCC(/C)=C\CNC1=NC=NC2=C1N=CN2 UZKQTCBAMSWPJD-UQCOIBPSSA-N 0.000 description 1
- -1 trans-zeatin nucleoside Chemical class 0.000 description 1
Landscapes
- Cultivation Of Plants (AREA)
Abstract
The invention discloses a kind of method of the method for improving blueberry tissue culture test tube seedling seedling exercising planting percent, including minimal medium progressively add strong seedling culture in Fructus Lycopersici esculenti bottle, in the miniature stem section room of healthy and strong tissue cultured seedling the outer high density root culture of bottle and Miniature tissue-culture take root Seedling seedling exercising and transplanting.The Fructus Lycopersici esculenti culture medium of addition stabilizes its low ph value growing environment, and the Seedling of cultivation is more healthy and stronger than the Seedling of prior art；Change outdoor rooting for indoor outside sprout-cultivating-bottle, operational approach simplicity is taken root steady decrease management difficulty；Host material adopts perlite, and easily, when taking root and transplanting, operating efficiency is high, while material is easily obtained for lightweight carrying.Through the miniature Seedling of taking root of blue berry obtained in the technical program, effectively Seedling quantity is 2.5～3 times of former technology (using complete Seedling), and per Seedling root quantity up to 10, nursery stock is healthy and strong, rooting rate reaches more than 95%, greatly reduces seedling cost when blueberry tissue culture seedling cultivation and rooting is transplanted.
Description
Technical field
The invention belongs to plant seedling tissue culture raising technology field, and in particular to a kind of to improve blueberry tissue culture test tube seedling seedling exercising
The method of planting percent.
Background technology
Blue berry have the good reputation of " king of antioxidation, U.S. pupil fruit ", more next for the people from modern metropolitan cities of fast pace life
More there is health value.China's blueberry industry has begun to take shape through the fast development over nearly 15 years, but area and yield are also
The demand of domestic market far can not be met.At present, domestic blue berry seeling industry relies primarily on tissue culture technology, its technological difficulties collection
In in the bottle link such as strong sprout, outside sprout-cultivating-bottle, acclimatization and transplantses.Generally existing breeding coefficient is low, rooting rate is low, operation of transplanting seedlings
Efficiency low grade " three is low " problem (hereinafter referred to as " three is low " problem), substantially increases blueberry tissue culture seedling cost and nursery stock valency
Lattice, so as to constrain the development of blueberry industry.
The content of the invention
It is contemplated that a kind of method of the method for improving blueberry tissue culture test tube seedling seedling exercising planting percent of integrated innovation, Solutions of Systems
" three is low " problem that certainly prior art is present.
The technical solution adopted in the present invention is, a kind of side of the method for improving blueberry tissue culture test tube seedling seedling exercising planting percent
Method, aseptically carries out initial culture and subculture multiplication culture, is carried out on the basis of culture obtains aseptic Regenerated plant again
Hereinafter operate：
Step 1, the WPM culture medium to Regenerated plant employing addition Fructus Lycopersici esculenti carry out strong seedling culture, obtain healthy and strong bottle seedling；
Step 2, the outer miniature stem section high density root culture of indoor bottle is carried out to healthy and strong bottle seedling, prepare miniature taking root
Seedling；
Step 3, outdoor seedling exercising and transplanting and management are carried out to miniature Seedling of taking root, cultivate criterion group culture container Seedling.
The characteristics of of the invention, also resides in,
Strong seedling culture is carried out specifically according to following steps using the WPM culture medium of addition Fructus Lycopersici esculenti to Regenerated plant in step 1
Implement:
Step 1.1, Regenerated plant is inoculated on blue berry subculture medium, blue berry successive transfer culture based formulas are WPM+
TZR1mg/L+ Fructus Lycopersici esculenti 5g/L, need not adjust pH value, transfer 1 time；
Step 1.2, by blue berry subculture medium formula adjustment be WPM+TZR 0.5mg/L+ Fructus Lycopersici esculenti 10g/L, without the need for adjust
Section pH value, obtains sufficient amount of healthy and strong bottle seedling Jing after many switchings.
Regenerated plant is carried out cutting with 1～2 simple bud when strong seedling culture is operated using the WPM culture medium of addition Fructus Lycopersici esculenti
In stem section inoculation medium, culture vessel adopts 250ml Cans, per bottle of inoculation quantity 10～15.
The outer miniature stem section high density root culture of indoor bottle is carried out in step 2 to healthy and strong bottle seedling specifically according to following steps reality
Apply：
Step 2.1,2 kinds of solution of taking root of preparation：Take root nutritional solution (1/5WPM nutritive solutions) and hormone solution (1mg/mL's
IBA solution), the clip of blue berry strong sprout stem section and process：Bottle cap is opened in atmosphere, test tube seedling is pressed from both sides out with tweezers, cuts off base portion
Part with culture medium, then the stem section with 2 buds is cut into, collected with clean container, 3s is soaked in hormone solution at once and dragged for
Go out；
The cuttage of step 2.2, stem section：Matrix of taking root is inserted in plastics cutlery box, is taken root blue berry obtained in step 2.1 miniature
Stem section is placed in the plastics cutlery box for having contained matrix of taking root, per 100 plants or so of box, changes cuttage for punching cuttage；
Step 2.3, sealing are taken root：Fresh-keeping film phonograph seal is used immediately, temperature is placed in for 21 DEG C～28 DEG C, and illumination condition is 2000
Cultivate 21 days under the conditions of～3000lux photoenvironments.
Take root the preparation of nutritional solution in step 2 and sterilizing methods are：The WPM culture medium for preparing 1L is diluted with water to 5L, dilute
Solution after releasing adjusts pH value to 4.5, sterilizes.Cool down after sterilizing and obtain nutritional solution of taking root, it is standby；The hormone solution is matched somebody with somebody
Method processed is：IBA 1g or NAA 1g are weighed, is dissolved with the NaOH solution of a small amount of 1N, is settled to 1L, the solution after dilution is adjusted
Section pH value is cooled down after sterilizing standby to 5.0；The compound method of the matrix of taking root is：According to perlite and nutritional solution weight of taking root
Amount ratio 1：5, both is mixed and be obtained matrix of taking root.
The seedling exercising of the miniature Seedling of taking root of step 3 blueberry tissue culture and transplanting are specifically implemented according to following steps：
Step 3.1, seedling exercising：In 20～25 DEG C of temperature, illumination 2000lux, under 70～90% environmental condition of relative humidity, step
Rapid 2.3 Seedling cutlery box preservative films of taking root scratch one openning, and seedling exercising 1～2 day throws off preservative film afterwards completely, and water continuation seedling exercising 1
～2 days；
Step 3.2, transplanting：The miniature Seedling of taking root of blue berry is collected after completing seedling exercising in plastics box container, tweezers during transplanting
The miniature Seedling of taking root of blue berry that gripping is taken root is directly placed in the hole tray for containing transplanting medium, is placed in the seedbed with spray irrigation system
It is interior, moisturizing of irregularly spraying water；
Step 3.3, upper basin：When Plug seedling young sprout grows 4-5 blade, basin on tape base replanting, wherein, plastic tub mouth
Footpath 8-10cm, that is, moving into nursery carries out normal management.
The concrete preparation method of transplanting medium is as follows：70 parts of peat, 10 parts of perlite, gold zone are weighed according to mass percent
10 parts of soil, 8 parts of rice husk, 2 parts of Controlled Release Fertilizer are puddled uniformly, and soaking disinfection pours permeable standby before transplanting.
The invention has the beneficial effects as follows：In the bottle that the technical program is adopted the outer high density of strong sprout, indoor bottle concentrate take root, room
The methods such as outer acclimatization and transplantses, improve planting percent and work efficiency, and system solves " three is low " of blue berry industrialized tissue culture nursery and asks
Topic.Compared with existing similar technique, advantage is：1. step 1 Regenerated plant culture medium addition Fructus Lycopersici esculenti Organic substance carries out strong sprout training
Support, improve the robustness of test tube seedling, be more beneficial for taking root and strengthening its resistivity to poor environment；Blue berry is naturally raw
Belong to the plant of happiness strongly acidic soil condition when long, as Fructus Lycopersici esculenti is rich in organic acid, the culture medium for adding Fructus Lycopersici esculenti remains low
The buffer capacity of pH value is greatly enhanced, and the adverse effect for reducing un-added culture medium later stage pH rising can also save training every time
The trouble of acid-base value is adjusted during foster basigamy.2. the outer high density rooting technique of indoor bottle that step 2 is adopted, changes sterilizing in method
Mode, carries out in-house operation, simple and convenient；On environmental Kuznets Curves, indoor controllability is strong, and convenient management is intensive, so as to ensure which
The stability taken root；3. the outdoor acclimatization and transplantses of the miniature Seedling of taking root of step 3, should fit when taming without root than similar technique
Environment is answered to take root again, its adaptability to environmental change is greatly enhanced, and rooting rate reaches 95%, is higher by than domestic average level
More than 10 percentage points；4. step 3 takes root stem section only with 2 buds, and only this stage is just than the nursery stock of prior art (5～6 buds)
Breeding coefficient improves more than 2 times.5. take root using perlite as holder, material easily obtains lightweight, operation side during transplanting
Just, 0.8～10,000 plants can be transplanted for each person every day, transplanting efficiency is 2 times of domestic prior art, significantly reduces cost of labor.
Description of the drawings
Fig. 1 is miniature seedling rooting microphotograph, wherein, a is miniature seedling rooting microphotograph for one kind；B is another
Kind be miniature seedling rooting microphotograph, c be take root after survive situation picture, d is that interior is taken root picture on a large scale.
Specific embodiment
The present invention is described in detail with reference to the accompanying drawings and detailed description.
The present invention provides a kind of method for improving blueberry tissue culture test tube seedling seedling exercising planting percent, specifically according to following steps reality
Apply：Taking blue berry semi-lignified or non-lignifying tender tip carries out aseptic inoculation, Jing initial culture and subculture multiplication culture, obtain after
Carry out following operation on the basis of Dai Miao again：
The strong seedling culture of step 1, Regenerated plant；By the blue berry aseptic seedling into the fast breeding stage, strong seedling culture is carried out.It is strong
Seedling culture is divided into two stages to be carried out：The culture medium of first stage is consisted of：Minimal medium, hormone and organic additive.Base
Basal culture medium adopts WPM culture medium, and addition hormone is trans-zeatin nucleoside (TZR), and concentration is 1mg/L, and organic additive is
Fructus Lycopersici esculenti, concentration are 5g/L, need not adjust pH value.Hormone in medium formula adjustment is TZR 0.5mg/L, organic by second stage
Additive Fructus Lycopersici esculenti concentration is adjusted to 10g/L, need not adjust pH value.First stage transfers 1 time, and second stage switching is multiple, often
Secondary 1 month interval time of switching, until culture obtains sufficient amount of healthy and strong bottle seedling.Every time during switch over operation, it is desirable to cut band 2
The stem section of individual simple bud, culture vessel adopt 250ml Cans, per bottle of inoculation quantity 10-15.
In step 2, healthy and strong bottle seedling room, the outer high density root culture of bottle, prepares 2 kinds of solution of taking root：Take root nutritional solution (1/
5WPM nutritive solutions) and hormone solution (the IBA solution of 1mg/mL).1/5WPM compounding methods are：Prepare the WPM trainings of 1L
Foster base is diluted with water to 5L, and the solution after dilution adjusts pH value to 4.5, cools down after boiling sterilization, standby.Hormone solution
Compound method is：IBA 1g are weighed, is dissolved with the NaOH solution of a small amount of 1N, is settled to 1L, the solution after dilution is adjusted into pH value
To 5.0, cool down after boiling sterilization standby.
By clean perlite with nutritional solution of taking root according to part by weight 1:5 mix homogeneously, are put into disposable transparent cutlery box
Interior, volume is the 1/3 of cutlery box volume, that is, obtain matrix of taking root.By the blue berry aseptic seedling young sprout for growing fine, without the need for ultra-clean work
Platform is directly taken out from culture bottle, is cut off part of the base portion with culture medium, then is cut into the stem section with 1～2 bud, big after cutting
Amount stem section is put into immersion 3s in hormone solution and pulls out, is first punched with the waddy of pencil thickness, the stem section after leaching is placed directly within hole
Interior, cuttage is in matrix of taking root, per one plant of hole, common about 100 plants.Fresh-keeping film phonograph seal, is placed in temperature for 25 ± 1 DEG C, 2000 immediately
Cultivate 21 days under the conditions of～3000lux photoenvironments；The miniature Seedling of high density is obtained, as shown in figure 1, wherein a is miniature for one kind
Seedling rooting microphotograph；B is miniature seedling rooting microphotograph for another kind.
The seedling exercising of the miniature Seedling of taking root of step 3, blue berry and transplanting, in 25 ± 1 DEG C of temperature, illumination 2000lux, relative humidity 70
～90%, carry out transition seedling exercising 3～5 days.Transplanted after seedling exercising, specially：Peat 70% is weighed according to mass percent, it is precious
Pearl rock 15%, yellow soil 8%, rice husk 8%, 2% ratio of Controlled Release Fertilizer are puddled uniformly, fill 50 or 72 hole disks after soaking disinfection,
Pour permeable standby before transplanting.The blue berry Miniature tissue-culture Seedling cuttage taken root is gripped in hole tray with tweezers during transplanting, is placed in spray
In the seedbed of fill system, treat within 20～30 days or so that Seedling young sprout transplants seedbed normal management by growing.
In vitro culture is colonized to outdoor will be transplanted to during sufficient amount blue berry seedling under aseptic condition, in transplanting
When due in test tube seedling previously to have grown very fast and plant young tender, it is necessary to through strict slow humiture transition, ability
Adapt to external environmental condition.The healthy and strong degree of nursery stock growing way and whether is taken root extreme influence is tamed survival rate.
Blue berry grows suitable strongly acidic soil condition (pH value 4.2-5.5) naturally, and the culture of blueberry tissue culture Seedling is also required to low
PH value condition is maintaining the absorption of ferrum element etc., therefore culture medium requires the culture medium condition of stronger acids.In medium sterilization
When and incubation in, the excessive propagation for just having influence on blue berry Seedling of amplitude of variation of Medium Culture pH value condition and growth.In Fructus Lycopersici esculenti
Containing Acidic organic matters such as citric acids, the buffer capacity of the low pH of its maintenance is greatly enhanced, and the present invention is by blueberry tissue culture Seedling
In Subculture, progressively increment adds Fructus Lycopersici esculenti organic matter, maintains low ph condition in culture medium, stabilizes indigo plant in substrate
The nutrients such as the ferrum that the certain kind of berries needs, while the unknown organic nutrient in Fructus Lycopersici esculenti provides active nutritional for the growth of blue berry, improve
The robustness of blueberry tissue culture seedling, so as to improve the rooting rate of blueberry tissue culture Seedling.
Rooting rate and breeding coefficient are improve using indoor rooting technique, is easily taken using materials such as Fructus Lycopersici esculenti, perlites
Must, steady quality is tamed with complete seedling, improves resistance of the seedling to poor environment, has both solved former culturing young plants
Take root the double combination obstacle tamed again, so as to improve transplanting survival rate and planting percent.Before thoroughly solving, blueberry tissue culture is educated
The problems such as dipping in " three is low " that run into during root-inducing powder cuttage seedling exercising during seedling rooting outside room, suitable batch production is simply produced.
Through the miniature Seedling of taking root of blue berry obtained in the technical program, effective Seedling quantity is former technology (using complete Seedling)
2.5～3 times, per Seedling root quantity up to 10, nursery stock is healthy and strong, and rooting rate reaches more than 95%, due to changing cuttage for punching
Cuttage, matrix of taking root holder adopt perlite, and root system adhesion substrate is light, and when taking root and transplanting, operational approach is simple, efficiency
It is high, while material is easily obtained, the cost of transplanting of taking root when greatly reducing blueberry tissue culture nursery.
Claims (6)
1. a kind of method for improving blueberry tissue culture test tube seedling seedling exercising planting percent, aseptically carries out initial culture and subculture increases
Culture is grown, following operation is carried out on the basis of culture obtains aseptic Regenerated plant again：
Step 1, the WPM culture medium to Regenerated plant employing addition Fructus Lycopersici esculenti carry out strong seedling culture, obtain healthy and strong bottle seedling；
Step 2, the outer miniature stem section high density root culture of indoor bottle is carried out to healthy and strong bottle seedling, prepare miniature Seedling of taking root；
Step 3, outdoor seedling exercising and transplanting and management are carried out to miniature Seedling of taking root, cultivate criterion group culture container Seedling；
Characterized in that, in the step 1 to Regenerated plant using addition Fructus Lycopersici esculenti WPM culture medium carry out strong seedling culture specifically by
Implement according to following steps:
Step 1.1, Regenerated plant is inoculated on blue berry subculture medium, blue berry successive transfer culture based formulas are WPM+TZR 1mg/L+
Fructus Lycopersici esculenti 5g/L, need not adjust pH value, transfer 1 time；
Step 1.2, by blue berry subculture medium formula adjustment be WPM+TZR 0.5mg/L+ Fructus Lycopersici esculenti 10g/L, pH need not be adjusted
Value, obtains sufficient amount of healthy and strong bottle seedling Jing after many switchings.
2. it is according to claim 1 improve blueberry tissue culture test tube seedling seedling exercising planting percent method, it is characterised in that it is described right
Regenerated plant carries out the inoculation training of the stem section with 1～2 simple bud is cut when strong seedling culture is operated using the WPM culture medium of addition Fructus Lycopersici esculenti
In foster base, culture vessel adopts 250ml Cans, per bottle of inoculation quantity 10～15.
3. it is according to claim 1 improve blueberry tissue culture test tube seedling seedling exercising planting percent method, it is characterised in that the step
Carry out the outer miniature stem section high density root culture of indoor bottle specifically to implement according to following steps in rapid 2 to healthy and strong bottle seedling：
Step 2.1, the clip of blue berry strong sprout stem section and process：Prepare 2 kinds of solution of taking root：Take root nutritional solution and hormone solution；
Bottle cap is opened in air, test tube seedling is pressed from both sides out with tweezers, cut off part of the base portion with culture medium, then be cut into the stem section with 2 buds,
Collected with clean container, 3s is soaked in hormone solution at once and pulled out；
The cuttage of step 2.2, stem section：Matrix of taking root is inserted in plastics cutlery box, by blue berry obtained in step 2.1 miniature stem section of taking root
It is placed in the plastics cutlery box for having contained matrix of taking root, per 100 plants or so of box, changes cuttage for punching cuttage；
Step 2.3, sealing are taken root：Fresh-keeping film phonograph seal to be used immediately, is placed in temperature for 21 DEG C～28 DEG C, illumination condition is 2000～
Cultivate 21 days under the conditions of 3000lux photoenvironments.
4. it is according to claim 3 improve blueberry tissue culture test tube seedling seedling exercising planting percent method, it is characterised in that the step
The compound method of nutritional solution of taking root in rapid 2 is：The WPM culture medium for preparing 1L is diluted with water to 5L, and the solution after dilution adjusts pH
It is worth 4.5, sterilizing is cooled down after sterilizing and obtains nutritional solution of taking root, standby；The compound method of the hormone solution is：Weigh IBA
1g or NAA 1g, are dissolved with the NaOH solution of a small amount of 1N, are settled to 1L, the solution after dilution are adjusted pH value to 5.0, after sterilizing
Cooling is standby；The compound method of the matrix of taking root is：According to pearlite interstitial substance and nutritional solution part by weight 1 of taking root：5, by two
Person's mixing is obtained matrix of taking root.
5. it is according to claim 4 improve blueberry tissue culture test tube seedling seedling exercising planting percent method, it is characterised in that the step
The seedling exercising of the miniature Seedling of taking root of rapid 3 blueberry tissue culture and transplanting specifically is implemented according to following steps：
Step 3.1, seedling exercising：In 20～25 DEG C of temperature, illumination 2000lux, under 70～90% environmental condition of relative humidity, step
2.3 Seedling cutlery box preservative films of taking root scratch one openning, and seedling exercising 1～2 day throws off preservative film afterwards completely, and the continuation seedling exercising 1 of watering～
2 days；
Step 3.2, transplanting：The miniature Seedling of taking root of blue berry is collected completely after seedling exercising in plastics box container, is gripped with tweezers during transplanting
The miniature Seedling of taking root of blue berry taken root is directly placed in the hole tray for containing transplanting medium, is placed in the seedbed with spray irrigation system, no
Periodically water spray moisturizing；
Step 3.3, upper basin：When Plug seedling young sprout grows 4-5 blade, basin on tape base replanting, wherein, plastic tub bore 8-
10cm, that is, moving into nursery carries out normal management.
6. it is according to claim 5 improve blueberry tissue culture test tube seedling seedling exercising planting percent method, it is characterised in that the shifting
The concrete preparation method for planting substrate is as follows：70 parts of peat, 10 parts of perlite, 10 parts of yellow soil, rice are weighed according to mass percent
8 parts of shell, 2 parts of Controlled Release Fertilizer are puddled uniformly, and soaking disinfection pours permeable standby before transplanting.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510133364.1A CN104719163B (en) | 2015-03-25 | 2015-03-25 | A kind of method for improving blueberry tissue culture test tube seedling seedling exercising planting percent |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510133364.1A CN104719163B (en) | 2015-03-25 | 2015-03-25 | A kind of method for improving blueberry tissue culture test tube seedling seedling exercising planting percent |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104719163A CN104719163A (en) | 2015-06-24 |
| CN104719163B true CN104719163B (en) | 2017-04-05 |
Family
ID=53444038
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510133364.1A Expired - Fee Related CN104719163B (en) | 2015-03-25 | 2015-03-25 | A kind of method for improving blueberry tissue culture test tube seedling seedling exercising planting percent |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104719163B (en) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105802632A (en) * | 2016-03-17 | 2016-07-27 | 凯里学院 | Pollution-free blueberry soil pH regulating solution and use method thereof |
| CN106613859A (en) * | 2017-02-08 | 2017-05-10 | 合肥师范学院 | Cultivation method of sound seedlings of blueberry tissue culture seedlings |
| CN106888903A (en) * | 2017-02-08 | 2017-06-27 | 合肥师范学院 | A kind of blueberry tissue culture bare-root seeding field-transplanting method |
| CN109122319A (en) * | 2018-09-03 | 2019-01-04 | 江苏高航农业科技有限公司 | A kind of method that high clump blueberry Anther Culture obtains haplobiont |
| CN108935088A (en) * | 2018-09-17 | 2018-12-07 | 温州科技职业学院 | A method of improving blueberry explant radioinduction rate |
| CN109863932A (en) * | 2018-10-28 | 2019-06-11 | 陕西海棠生态农林股份有限公司 | A kind of method of nutritive cube cultivation Tissue-cultured apple seedling |
| CN111133982A (en) * | 2020-03-10 | 2020-05-12 | 肇庆市恒发农业科技有限公司 | Blueberry culture medium, preparation method and application |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102165920A (en) * | 2011-02-21 | 2011-08-31 | 浙江省萧山棉麻研究所 | Tissue culturing method for regenerating somatic cells of aquatic iris |
| CN104255463A (en) * | 2014-09-12 | 2015-01-07 | 南京通泽农业科技有限公司 | Rapid propagation method of suspension cells of Epilobium angustifolium Linn. |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103548681B (en) * | 2013-10-31 | 2016-02-24 | 浙江农林大学天目学院 | The substratum of rapid evoking adventive bud in a kind of spring stem of noble dendrobium tissue culture |
-
2015
- 2015-03-25 CN CN201510133364.1A patent/CN104719163B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102165920A (en) * | 2011-02-21 | 2011-08-31 | 浙江省萧山棉麻研究所 | Tissue culturing method for regenerating somatic cells of aquatic iris |
| CN104255463A (en) * | 2014-09-12 | 2015-01-07 | 南京通泽农业科技有限公司 | Rapid propagation method of suspension cells of Epilobium angustifolium Linn. |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104719163A (en) | 2015-06-24 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104719163B (en) | A kind of method for improving blueberry tissue culture test tube seedling seedling exercising planting percent | |
| CN102550411B (en) | Method for producing pre-basic seeds of potatoes | |
| CN103931492B (en) | The tissue culture fast seedling-cultivating method of apple rootstock M9 | |
| CN102422816A (en) | Method for rapidly culturing shoot tips in vitro | |
| CN102577976B (en) | Simple tissue culture method for broussonetia papyrifera | |
| CN105284620B (en) | A kind of method that Superearly peach bybrid embryo saves seedling | |
| CN102144547A (en) | Method for quickly breeding and transplanting grape stock unit | |
| CN103734009B (en) | Tissue culture method of anthurium | |
| CN103875515B (en) | A kind of blueberry tissue culture outside sprout-cultivating-bottle radication method | |
| CN112189566B (en) | Rapid breeding method of cherry seedlings for stocks | |
| CN104686338A (en) | In-vitro culture technique of anther of angelica dahurica | |
| CN104396759B (en) | The method that ash tree tissue cultures is bred fast | |
| CN104137779A (en) | Method for regenerating sapium japonicum plant by inducing sapium japonicum stem rapidly | |
| CN105638455B (en) | A kind of method and culture medium that Drying pepper haplobiont is obtained by Anther Culture | |
| CN105532467B (en) | Endangered rhododendron molle in-vitro tissue culture propagation and preservation method | |
| CN101695280B (en) | Tissue culture and rapid propagation method of raspberries | |
| CN102907326A (en) | Tissue culture propagation method for Medicagao Sativa L. | |
| CN105145363B (en) | It is a kind of to significantly improve the method that China fir tissue culture produces emergence rate | |
| CN106973796A (en) | A kind of tissue cultivating and seedling method of Idesia polycarpa | |
| CN106258993A (en) | A kind of blueberry tissue culture method | |
| CN107278686A (en) | A kind of American red-maple tissue culture outside sprout-cultivating-bottle radication agent and method for culturing seedlings | |
| CN103843664B (en) | Lycium exsertum tissue is cultivated and method for quickly breeding | |
| CN103229721B (en) | Tissue culture propagation method of Gynura formosana | |
| CN103039360B (en) | Method for quickly propagating leeka through tissue culture | |
| CN103155869A (en) | Sweet cherry rootstock Colt tissue culture method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| EXSB | Decision made by sipo to initiate substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20170405 Termination date: 20210325 |