CN104703700B - 用于核酸测序的方法和试剂盒 - Google Patents

用于核酸测序的方法和试剂盒 Download PDF

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Publication number
CN104703700B
CN104703700B CN201380051830.0A CN201380051830A CN104703700B CN 104703700 B CN104703700 B CN 104703700B CN 201380051830 A CN201380051830 A CN 201380051830A CN 104703700 B CN104703700 B CN 104703700B
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nanochannel
sensitive
polynucleotide
dna
mensuration region
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Chinese (zh)
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CN104703700A (zh
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乔纳森·奥汉隆
克里斯托弗·亚当斯
乔·赫德利
萨姆·怀特豪斯
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QuantumDx Group Ltd
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
    • G01N33/48721—Investigating individual macromolecules, e.g. by translocation through nanopores
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads or physically stretching molecules
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y15/00—Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Nanotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Hematology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Food Science & Technology (AREA)
  • Urology & Nephrology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Fluid Mechanics (AREA)
  • Dispersion Chemistry (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CN201380051830.0A 2012-08-06 2013-08-05 用于核酸测序的方法和试剂盒 Expired - Fee Related CN104703700B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261680212P 2012-08-06 2012-08-06
US61/680,212 2012-08-06
PCT/IB2013/002168 WO2014024041A1 (en) 2012-08-06 2013-08-05 Method and kit for nucleic acid sequencing

Publications (2)

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CN104703700A CN104703700A (zh) 2015-06-10
CN104703700B true CN104703700B (zh) 2018-01-12

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Country Link
US (1) US20150276709A1 (https=)
EP (1) EP2879793A1 (https=)
JP (1) JP2015528282A (https=)
CN (1) CN104703700B (https=)
HK (1) HK1211260A1 (https=)
IN (1) IN2015DN01300A (https=)
WO (1) WO2014024041A1 (https=)

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US9857328B2 (en) 2014-12-18 2018-01-02 Agilome, Inc. Chemically-sensitive field effect transistors, systems and methods for manufacturing and using the same
US10020300B2 (en) 2014-12-18 2018-07-10 Agilome, Inc. Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids
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KR102713216B1 (ko) * 2015-06-25 2024-10-02 로스웰 엠이 아이엔씨. 바이오분자 센서들 및 방법들
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KR102763291B1 (ko) 2016-01-28 2025-02-04 로스웰 엠이 아이엔씨. 대량 병렬 ｄｎａ 시퀀싱 장치
CN106191230A (zh) * 2016-02-02 2016-12-07 杭州格磊思沃科技有限公司 用氨基酸结合脱氧核苷酸进行dna测序的方法
CA3053103A1 (en) 2016-02-09 2017-08-17 Roswell Biotechnologies, Inc. Electronic label-free dna and genome sequencing
US10597767B2 (en) 2016-02-22 2020-03-24 Roswell Biotechnologies, Inc. Nanoparticle fabrication
CN109661274A (zh) * 2016-04-04 2019-04-19 卡尤迪生物科技（北京）有限公司 用于加热生物样品的系统和方法
WO2017201081A1 (en) 2016-05-16 2017-11-23 Agilome, Inc. Graphene fet devices, systems, and methods of using the same for sequencing nucleic acids
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CN110431148A (zh) 2017-01-10 2019-11-08 罗斯威尔生命技术公司 用于dna数据存储的方法和系统
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EP3622086A4 (en) 2017-05-09 2021-04-21 Roswell Biotechnologies, Inc BINDING PROBE CIRCUITS FOR MOLECULAR SENSORS
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CN107475072B (zh) * 2017-08-24 2020-12-11 浙江理工大学 基于三维石墨烯界面电极的细胞动态特性监测系统及方法
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US11099136B2 (en) * 2017-12-22 2021-08-24 Regents Of The University Of Minnesota 3D graphene optical sensors and methods of manufacture
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DE102018207106B4 (de) * 2018-05-08 2024-06-13 Robert Bosch Gmbh Mikrofluidische Sequenzierungsvorrichtung zum Vervielfältigen und Trennen von Molekülketten und Verfahren zum Trennen von aus einer Amplifikationsreaktion gewonnen Molekülketten
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Also Published As

Publication number Publication date
IN2015DN01300A (https=) 2015-07-03
HK1211260A1 (en) 2016-05-20
EP2879793A1 (en) 2015-06-10
CN104703700A (zh) 2015-06-10
US20150276709A1 (en) 2015-10-01
WO2014024041A1 (en) 2014-02-13
JP2015528282A (ja) 2015-09-28

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