CN104686803A - Compound micro-ecological preparation for promoting growth of animals and improving production performance of animals - Google Patents

Compound micro-ecological preparation for promoting growth of animals and improving production performance of animals Download PDF

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Publication number
CN104686803A
CN104686803A CN201510073890.3A CN201510073890A CN104686803A CN 104686803 A CN104686803 A CN 104686803A CN 201510073890 A CN201510073890 A CN 201510073890A CN 104686803 A CN104686803 A CN 104686803A
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animals
lactobacillus acidophilus
animal
compound micro
ecological preparation
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岳道友
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Abstract

The invention provides a compound micro-ecological preparation for promoting growth of animals and improving production performance of animals. The compound micro-ecological preparation is prepared from the following raw materials: vitamin C, a transfer factor extracted from animal serum, methionine, lysine, lactobacillus acidophilus, bacillus subtilis and rice bran. The compound micro-ecological preparation can improve the intestinal environments of the animals, improve the production performance of the animals and enhance the immunity of the animals. The selected beneficial bacteria are lactobacillus acidophilus and bacillus subtilis from animal bodies, the animal serum is collected and extracted from healthy animals after slaughtering, vitamin C, methionine and lysine are added to improve the amount of beneficial bacteria in intestinal tracts and inhibit the growth of harmful bacteria by virtue of reasonable production proportions, and the growth of the animals is promoted and the immunity of the animals is improved by providing nutrient substances including growth factors, amino acid, vitamins and the like for the animals. Moreover, the raw materials of the formula of the compound micro-ecological preparation provided by the invention are acidic, thereby greatly solving the problems that vitamin C is instable and becomes ineffective easily.

Description

A kind of compound micro-ecological preparation promoting growth of animal and improve breeding performonce fo animals
Technical field
The present invention relates to a kind of compound micro-ecological preparation promoting growth of animal and improve breeding performonce fo animals.
Background technology
Research shows, beneficial bacterium is as probiotics extensive use in animal-breeding, and beneficial bacterium has nontoxicity, free of contamination advantage in production, and beneficial bacterium growth and breeding technology is ripe, and how allowing beneficial bacterium serve the mankind better becomes hot issue.And animal blood serum is also considered to containing abundant nutriment, and kind is also quite abundant, and animal blood serum aboundresources, if animal blood serum is well utilized, the waste of animal blood would not be caused, can economize on resources.
Aquaculture is managed in fowl poultry kind large-scale production and breeding environment is significantly improved; also the disease of more complexity can be brought in breeding process; the difficult problems such as the state of an illness is not single; in order to prevent disease better; ensure the health of animal and the health of human diet, research comprehensively can improve the health status of animal, the non-toxic and non-pollution product of reduction Animal diseases Probability is necessary.
Summary of the invention
The object of this invention is to provide a kind of compound micro-ecological preparation promoting growth of animal and improve breeding performonce fo animals.
For achieving the above object, the present invention is by the following technical solutions:
A kind of compound micro-ecological preparation promoting growth of animal and improve breeding performonce fo animals, containing, for example the raw material of lower weight in every 100g compound micro-ecological preparation: transfer factor 5 ~ 10g, methionine 1 ~ 3g, lysine 1 ~ 3g that vitamin C 1 ~ 3g, animal blood serum extract, live lactobacillus acidophilus number 2x10 8-2x10 9cFU/g, bacillus subtilis viable bacteria 2x10 8-2x10 9cFU/g, all the other are rice bran.
Described animal blood serum is Swine serum.
Contain in every 100g compound formulation: vitamin C 2g, the transfer factor 6g that animal blood serum extracts, methionine 2g, lysine 2g, live lactobacillus acidophilus number 5x108CFU/g, bacillus subtilis viable count 5x108CFU/g, surplus is rice bran.
Step one, by Lactobacillus acidophilus and the independent inoculated and cultured of Lactobacillus acidophilus, Lactobacillus acidophilus's liquid anaerobic fermentation, Lactobacillus acidophilus's liquid aerobic fermentation, is cultured to exponential phase 0.5 × 10 8cFU/mL to 1.0 × 10 8during CFU/mL only, the cultivation temperature of Lactobacillus acidophilus is 29-32 DEG C, the cultivation temperature of bacillus subtilis is 35-37 DEG C;
Step 2, take rice bran as carrier, add Lactobacillus acidophilus prepared by step one, transfer factor that bacillus subtilis mixed bacteria liquid, animal blood serum extract, vitamin C and methionine, lysine, then carry out concentrate drying, make live lactobacillus acidophilus number, bacillus subtilis viable bacteria reaches 2x10 8-2x10 9cFU/g.
Methionine is sulfur-bearing essential amino acid.When lacking methionine, anorexia can be caused, growth slow down or do not put on weight, the phenomenon such as kidney enlargement and the accumulation of liver iron.Because the lysine content in cereal foods is very low, and be easily destroyed in process and lack.
The symptom lacking lysine comprises fatigue, weak, feels sick, and vomiting is dizzy, does not have appetite, hypoevolutism, anaemia etc.
The C that is deficient in vitamin can cause scurvy.In vivo, vitamin C is a kind of antioxidant, and protection health avoids the threat of free radical, can be oxidized by air and light, and its aqueous solution is unstable, is oxidized to hydroascorbic acid very soon, especially oxidized very soon in neutrality or alkaline solution.Meeting the metal ions such as light, heat, iron and copper all can accelerated oxidation.Stable slaine can be formed.Chance air, heating, alkaline solution all easily cause rotten.
Owing to slaughtering animal, beneficial bacterium selected by a large amount of serum is from the lactobacillus acidophilus in animal body and bacillus subtilis, animal blood serum is slaughtered rear collection from healthy animal and is extracted, the nutriment of animal can be used as, can also promote that the immune system of animal is perfect, add vitamin C, methionine and lysine again, by reasonable production proportioning, improve intestinal beneficial bacterium quantity, suppress the growth of harmful bacteria, by providing the nutriments such as growth factor, amino acid, vitamin to animal, improving breeding performonce fo animals, improving its immunity.The present invention utilizes beneficial bacterium can improve animal intestinal environment, the features such as metabolic nutrition material, the transfer factor that animal blood serum extracts can improve the immunity of animal, and amino acid and vitamin can supplement the deficiency of animal self content, strengthen the resistance to disease especially viral disease.Vitamin C is stablized in acid condition, and meets Sum decomposition in alkali, and the formula material attribute in the present invention is acid, this problem significantly solving vitamin C instability, easily lost efficacy.In fermentation of bacillus subtilis liquid, add rice bran carry out the uniformity coefficient that concentrate drying provides bacterium.
Detailed description of the invention
The transfer factor that raw material vitamin C in test, animal blood serum extract, methionine, lysine, lactobacillus acidophilus, bacillus subtilis are commercially available.
embodiment 1
Promote growth of animal and the compound micro-ecological preparation improving breeding performonce fo animals, containing, for example the raw material of lower weight in every 100g compound micro-ecological preparation: transfer factor 5 ~ 10g, methionine 1 ~ 3g that vitamin C 1 ~ 3g, animal blood serum extract, lysine 1 ~ 3g, live lactobacillus acidophilus number 2x10 8-2x10 9cFU/g, bacillus subtilis viable bacteria 2x10 8-2x10 9cFU/g,
All the other are rice bran.
Described animal blood serum is Swine serum.
Contain in every 100g compound formulation: vitamin C 2g, the transfer factor 6g that animal blood serum extracts, methionine 2g, lysine 2g, live lactobacillus acidophilus number 5x108CFU/g, bacillus subtilis viable count 5x108CFU/g, surplus is rice bran.
Promote growth of animal and the preparation method of the compound micro-ecological preparation of raising breeding performonce fo animals, comprise the steps:
Step one, by Lactobacillus acidophilus and the independent inoculated and cultured of bacillus subtilis, culture technique is cellar culture, and the cultivation temperature of Lactobacillus acidophilus is 29-32 DEG C, the cultivation temperature of bacillus subtilis is 35-37 DEG C; Step 2, take rice bran as carrier, add Lactobacillus acidophilus prepared by step one, transfer factor that bacillus subtilis mixed bacteria liquid, animal blood serum extract, vitamin C and methionine, lysine, then carry out concentrate drying, make live lactobacillus acidophilus number, bacillus subtilis viable bacteria reaches 2x10 8-2x10 9cFU/g.
During use, product of the present invention is added in animal feed.Every 1000kg feed adds this product 10-20kg, is applicable to all animals.
embodiment 2
Concrete steps are as embodiment 1, a kind of compound micro-ecological preparation promoting growth of animal and improve breeding performonce fo animals, containing, for example the raw material of lower weight in every 1000Kg compound micro-ecological preparation: transfer factor 60 Kg, methionine 3 0Kg, lysine 10 Kg that vitamin C 10Kg, animal blood serum extract, live lactobacillus acidophilus number 2x10 9cFU/g, bacillus subtilis viable bacteria 2x10 8cFU/g, all the other are rice bran.
Described animal blood serum is Swine serum.
Promote growth of animal and the preparation method of the compound micro-ecological preparation of raising breeding performonce fo animals, comprise the steps:
Step one, by Lactobacillus acidophilus and the independent inoculated and cultured of bacillus subtilis, culture technique is cellar culture, and the cultivation temperature of Lactobacillus acidophilus is 30 DEG C, the cultivation temperature of bacillus subtilis is 37 DEG C;
Step 2 is carrier with rice bran, add Lactobacillus acidophilus prepared by step one, transfer factor that bacillus subtilis mixed bacteria liquid, animal blood serum extract, vitamin C and methionine, lysine, then carry out concentrate drying, make live lactobacillus acidophilus number 2x10 8-2x10 9cFU/g, bacillus subtilis viable bacteria 2x10 8-2x10 9cFU/g.
embodiment 3
Concrete steps are as embodiment 1, a kind of compound micro-ecological preparation promoting growth of animal and improve breeding performonce fo animals, containing, for example the raw material of lower weight in every 100g compound micro-ecological preparation: contain in every 1000Kg compound formulation: vitamin C 20Kg, the transfer factor 60Kg that animal blood serum extracts, methionine 20Kg, lysine 20Kg, live lactobacillus acidophilus number 2x10 8-2x10 9cFU/g, bacillus subtilis viable bacteria 2x10 8-2x10 9cFU/g, surplus is rice bran.
Described animal blood serum is Swine serum.
During use, product of the present invention is added in animal feed.Every 1000kg feed adds this product 10kg, is applicable to all animals, and one week spice once.
embodiment 4
Concrete steps are as embodiment 1, a kind of compound micro-ecological preparation promoting growth of animal and improve breeding performonce fo animals, containing, for example the raw material of lower weight in every 1000Kg compound micro-ecological preparation: transfer factor 50Kg, methionine 10kg, lysine 10Kg that vitamin C 10Kg, animal blood serum extract, live lactobacillus acidophilus number 2x10 8-2x10 9cFU/g, bacillus subtilis viable bacteria 2x10 8-2x10 9cFU/g, all the other are rice bran.
Described animal blood serum is Swine serum.
During use, product of the present invention is added in animal feed.Every 1000kg feed adds this product 10kg, is applicable to growth of animal fattening period, egg-laying bird, and egg-laying peak uses two weeks continuously, also can Long-Time Service.
embodiment 5-15
Specific implementation method is as contained composition as shown in table 1 in the raw material in embodiment 2,1000kg preparation.
clinical testing 1
1, trial
Test site: breeding layer chicken field, Henan; Test period two months, wherein first week is preliminary trial period, records the egg number of each repetition, and suitably adjusts according to statistics, makes control group and test group laying rate difference between preliminary trial period not remarkable.
Select the blue laying hen 600 in the sea of 200 age in days health, be divided into 2 process at random, each process divides 3 repetitions, each repetition 100.Control group A is fed basal diet, and experiment group B is fed in daily ration and added the embodiment of the present invention 2 product by 1%.
Test laying hen hen house is the confined poultry house of manual control environment, and feeding manner is that four layers of staged are raised in cages, and every cage 3 chickens, are fed by special messenger, and day feeds 3 times, and chicken free choice feeding, drinking-water, every day, light application time was 16 hours, and light application time is constant.
2, the observation of experimental performance index counts by repeating to record egg number, egg size, feed intake, damaged number, secondary bad egg number (the uneven and lopsided egg of pigmentation, shell egg, husky lime-preserved egg, soft-shelled egg) and dead washing in a pan with record every day from just trying the phase, calculates laying rate, feedstuff-egg ratio, breakage rate and secondary egg rate.Observe the state of mind of chicken, appetite and defecation situation every day.
Be 100%.
3, result of the test test result is as shown in table 2.
Note: same column lowercase identical table shows significant difference (P<0.05); Same column lowercase difference represents difference not significantly (P>0.05).
4, discuss
In sum, add in layer diets that product of the present invention can improve laying rate, improves food conversion ratio, reduces diarrhea rate, there is obvious effect the aspect such as to increase economic efficiency.
clinical testing 2
1, experimental design
600 1 age in days AA Broiler chicks, are divided into 2 groups immediately, often organize 3 repetitions, each repetition 100, A group is control group, only feeds basal diet, adds product 1% of the present invention in the daily ration of B group respectively.
2, feeding and management
Before test, often group is weighed, and in feeding process, observes chicken health condition every day, record death toll, in time its feed consumption rate of deduction.Free choice feeding, drinking-water, carry out immunity inoculation by chicken house normal procedure.Weigh respectively in 14 ages in days, add up feed consumption, statistical analysis process is carried out to the data obtained.
3, result of the test test result is as shown in table 3.
4, discuss
In sum, add product of the present invention in broiler diet and can improve daily gain, reduce feedstuff-meat ratio, in increasing economic efficiency etc., have obvious effect.
clinical testing 3
1, experimental design
Select age, parity, age in days of giving milk, the output of milk close as far as possible, the American Houstain cow 40 healthy, body condition is good, is divided into two groups, test group and each 21 of control group at random.
One week this test preliminary trial period, from February 25th, 2013 to April 1 for just to try the phase, 35 days full phases.
2, trial control group fed basal diet, test group adds product 1% of the present invention on equal daily ration basis.Be responsible for by special messenger for examination milk cow test group and control group, feed mode is consistent with feeding and management.To off-test from preliminary trial period, every day viewing test ox appetite and health status, weigh every day and record the lactation amount (this plant is full-automatic conputer controlled) of every ox.
3, result of the test is as shown in table 4.
Product of the present invention significantly improves the milk yield of lactating cow as can be seen from Table 4.

Claims (4)

1. one kind promotes growth of animal and the compound micro-ecological preparation improving breeding performonce fo animals, it is characterized in that, containing, for example the raw material of lower weight in every 100g compound micro-ecological preparation: transfer factor 5 ~ 10g, methionine 1 ~ 3g, lysine 1 ~ 3g that vitamin C 1 ~ 3g, animal blood serum extract, live lactobacillus acidophilus number 2x10 8-2x10 9cFU/g, bacillus subtilis viable bacteria 2x10 8-2x10 9cFU/g, all the other are rice bran.
2. the compound micro-ecological preparation promoting growth of animal and improve breeding performonce fo animals as claimed in claim 1, is characterized in that: described animal blood serum is Swine serum.
3. the compound micro-ecological preparation promoting growth of animal and improve breeding performonce fo animals as claimed in claim 1, it is characterized in that: contain in every 100g compound formulation: vitamin C 2g, the transfer factor 6g that animal blood serum extracts, methionine 2g, lysine 2g, live lactobacillus acidophilus number 5x10 8cFU/g, bacillus subtilis viable count 5x10 8cFU/g, surplus is rice bran.
4. the promotion growth of animal as described in claim 1,2 or 3 and the preparation method of the compound micro-ecological preparation of raising breeding performonce fo animals, comprise the steps:
Step one, by Lactobacillus acidophilus and the independent inoculated and cultured of Lactobacillus acidophilus, Lactobacillus acidophilus's liquid anaerobic fermentation, Lactobacillus acidophilus's liquid aerobic fermentation, is cultured to exponential phase 0.5 × 10 8cFU/mL to 1.0 × 10 8during CFU/mL only, the cultivation temperature of Lactobacillus acidophilus is 29 DEG C-32 DEG C, the cultivation temperature of bacillus subtilis is 35 DEG C-37 DEG C;
Step 2, take rice bran as carrier, add Lactobacillus acidophilus prepared by step one, transfer factor that bacillus subtilis mixed bacteria liquid, animal blood serum extract, vitamin C and methionine, lysine, then carry out concentrate drying, make live lactobacillus acidophilus number, bacillus subtilis viable bacteria reaches 2x10 8-2x10 9cFU/g.
CN201510073890.3A 2015-02-11 2015-02-11 Compound micro-ecological preparation for promoting growth of animals and improving production performance of animals Pending CN104686803A (en)

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