CN104662170A - 通过使用流式细胞术的rna测量快速检测t细胞活化 - Google Patents

通过使用流式细胞术的rna测量快速检测t细胞活化 Download PDF

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Publication number
CN104662170A
CN104662170A CN201380045036.5A CN201380045036A CN104662170A CN 104662170 A CN104662170 A CN 104662170A CN 201380045036 A CN201380045036 A CN 201380045036A CN 104662170 A CN104662170 A CN 104662170A
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China
Prior art keywords
cell
cells
probe
cytokine
peptide
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CN201380045036.5A
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English (en)
Chinese (zh)
Inventor
Y·布希金
M·L·真纳罗
桑杰·泰尔吉
理查德·皮纳
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Rutgers State University of New Jersey
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Rutgers State University of New Jersey
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Priority to CN201810454480.7A priority Critical patent/CN108624654A/zh
Publication of CN104662170A publication Critical patent/CN104662170A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6841—In situ hybridisation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1404—Handling flow, e.g. hydrodynamic focusing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6863—Cytokines, i.e. immune system proteins modifying a biological response such as cell growth proliferation or differentiation, e.g. TNF, CNF, GM-CSF, lymphotoxin, MIF or their receptors
    • G01N33/6866—Interferon
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6863—Cytokines, i.e. immune system proteins modifying a biological response such as cell growth proliferation or differentiation, e.g. TNF, CNF, GM-CSF, lymphotoxin, MIF or their receptors
    • G01N33/6869—Interleukin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/107—Nucleic acid detection characterized by the use of physical, structural and functional properties fluorescence

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Cell Biology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Dispersion Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
CN201380045036.5A 2012-06-27 2013-06-26 通过使用流式细胞术的rna测量快速检测t细胞活化 Pending CN104662170A (zh)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201810454480.7A CN108624654A (zh) 2012-06-27 2013-06-26 通过使用流式细胞术的rna测量快速检测t细胞活化

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201261665231P 2012-06-27 2012-06-27
US61/665,231 2012-06-27
US201361784802P 2013-03-14 2013-03-14
US61/784,802 2013-03-14
PCT/US2013/047774 WO2014004609A2 (en) 2012-06-27 2013-06-26 Rapid assays for t-cell activation by rna measurements using flow cytometry

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CN201810454480.7A Division CN108624654A (zh) 2012-06-27 2013-06-26 通过使用流式细胞术的rna测量快速检测t细胞活化

Publications (1)

Publication Number Publication Date
CN104662170A true CN104662170A (zh) 2015-05-27

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CN201380045036.5A Pending CN104662170A (zh) 2012-06-27 2013-06-26 通过使用流式细胞术的rna测量快速检测t细胞活化
CN201810454480.7A Pending CN108624654A (zh) 2012-06-27 2013-06-26 通过使用流式细胞术的rna测量快速检测t细胞活化

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Country Status (12)

Country Link
US (2) US10900072B2 (https=)
EP (2) EP2867374B1 (https=)
JP (1) JP6464081B2 (https=)
KR (1) KR20150036186A (https=)
CN (2) CN104662170A (https=)
AU (2) AU2013280416B2 (https=)
BR (1) BR112014032677A2 (https=)
CA (1) CA2877933A1 (https=)
IN (1) IN2015KN00073A (https=)
RU (1) RU2671707C2 (https=)
WO (1) WO2014004609A2 (https=)
ZA (1) ZA201500239B (https=)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108732082A (zh) * 2018-06-07 2018-11-02 北京唯公医疗技术有限公司 细胞分群方法
CN111999235A (zh) * 2020-07-03 2020-11-27 青岛农业大学 一种利用流式细胞术快速检测有活力的食用菌原生质体数量的方法

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114717296B (zh) 2012-02-03 2025-04-04 加州理工学院 多路生化测定中信号的编码和解码
EP3901243A1 (en) 2012-08-03 2021-10-27 California Institute of Technology Multiplexing and quantification in pcr with reduced hardware and requirements
CN106198358B (zh) * 2016-06-24 2019-02-15 北京大学 基于"石英杯中激发型"流式细胞分选仪的染色体分选方法
US12454720B2 (en) 2018-04-17 2025-10-28 ChromaCode, Inc. Methods and systems for multiplex analysis
US12203129B2 (en) * 2018-07-03 2025-01-21 ChromaCode, Inc. Formulations and signal encoding and decoding methods for massively multiplexed biochemical assays
JP7457572B2 (ja) * 2019-05-27 2024-03-28 住友化学株式会社 光学フィルムの反りの測定方法、及び、測定装置
US20220308061A1 (en) * 2019-09-03 2022-09-29 The Regents Of The University Of California Method to sequence mrna in single cells in parallel with quantification of intracellular phenotype
WO2021083807A1 (en) * 2019-10-29 2021-05-06 Universitaet Heidelberg Artificial antigen presenting molecules and their uses
RU2766781C1 (ru) * 2021-03-03 2022-03-15 Федеральное государственное бюджетное учреждение "Национальный медицинский исследовательский центр кардиологии" Министерства здравоохранения Российской Федерации (ФГБУ "НМИЦ кардиологии" Минздрава России) Способ выявления поражения сосудистого эндотелия
WO2022271272A1 (en) * 2021-06-22 2022-12-29 Nantcell, Inc. Rapid test for patient cd8 t-cell activation, systems and methods

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005118788A2 (en) * 2004-05-27 2005-12-15 The Trustees Of The University Of Pennsylvania Novel artificial antigen presenting cells and uses therefor
US7879609B2 (en) * 1996-10-01 2011-02-01 Geron Corporation Regulatory segments of the human gene for telomerase reverse transcriptase

Family Cites Families (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4994369A (en) * 1987-12-15 1991-02-19 The Board Of Trustees Of The Leland Stanford Jr. Univ. T-cell activation related gene
US5866331A (en) 1995-10-20 1999-02-02 University Of Massachusetts Single molecule detection by in situ hybridization
IL129222A0 (en) * 1996-10-01 2000-02-17 Geron Corp Telomerase reverse transcriptase
US7514232B2 (en) * 1996-12-06 2009-04-07 Becton, Dickinson And Company Method for detecting T cell response to specific antigens in whole blood
RU2258710C2 (ru) 1999-03-09 2005-08-20 Займодженетикс, Инк. Новый цитокин zalpha11-лиганд
KR100743640B1 (ko) 1999-03-09 2007-07-27 지모제넥틱스, 인코포레이티드 신규한 사이토킨 ｚａｌｐｈａ 11 리간드
US20040033517A1 (en) 2002-04-19 2004-02-19 Wolfram Ruf Compositions and methods relating to endothelial cell signaling using the protease activated receptor (PAR1)
WO2004015070A2 (en) * 2002-08-08 2004-02-19 Baylor College Of Medicine Isolation and identification of t cells
DE102005020754B3 (de) 2005-05-02 2007-01-11 Altana Pharma Ag Zelluläres Assayverfahren zur Identifizierung von PKCtheta-Inhibitoren
DK2500439T4 (da) 2005-06-20 2017-11-13 Advanced Cell Diagnostics Inc Kits og produkter til detektering af nukleinsyrer i individuelle celler og til identifikation af sjældne celler fra store heterogene cellepopulationer
US20090081688A1 (en) * 2005-06-20 2009-03-26 Advanced Cell Diagnostics Methods of detecting nucleic acids in individual cells and of identifying rare cells from large heterogeneous cell populations
EP1760089B1 (en) 2005-09-05 2009-08-19 Immatics Biotechnologies GmbH Tumor-associated peptides binding to human leukocyte antigen (HLA) class I or II molecules and related anti-cancer vaccine
US8114982B2 (en) * 2006-12-06 2012-02-14 Albert Einstein College Of Medicine Of Yeshiva University Multi-microRNA methods and compositions
EP3620465B1 (en) 2007-07-03 2025-02-19 Dako Denmark A/S Improved methods for generation, labeling and use of mhc multimers
JP2010004750A (ja) * 2008-06-24 2010-01-14 Institute For Rheumatic Diseases Co Ltd 自己免疫疾患による組織傷害の発症または発症可能性の診断方法、およびその利用
EP2186827A1 (en) * 2008-11-14 2010-05-19 HS LifeSciences Ltd. Surrogate marker directed cDNA cloning of selectively induced mRNAs
ES2363545B2 (es) * 2010-01-26 2012-04-18 Universidade De Santiago De Compostela Método para identificar y purificar células t reguladoras naturales humanas (ntreg).
DE102010037622B4 (de) * 2010-09-17 2012-07-12 Lophius Biosciences Gmbh Verfahren zum Nachweis, Differenzieren und Quantifizieren von T-Zellpopulationen mittels der Reverse Transkription quantitativen Real-Time PCR (RT-qPCR) Technologie
AR084754A1 (es) * 2010-12-30 2013-06-05 Anthrogenesis Corp Composiciones que comprenden celulas madre de placenta y plasma rico en plaquetas, y sus metodos de uso
US9902990B2 (en) * 2011-05-27 2018-02-27 The University Of British Columbia Microfluidic cell trap and assay apparatus for high-throughput analysis
JP5344007B2 (ja) * 2011-06-30 2013-11-20 株式会社デンソー 近距離無線通信装置

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7879609B2 (en) * 1996-10-01 2011-02-01 Geron Corporation Regulatory segments of the human gene for telomerase reverse transcriptase
WO2005118788A2 (en) * 2004-05-27 2005-12-15 The Trustees Of The University Of Pennsylvania Novel artificial antigen presenting cells and uses therefor

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108732082A (zh) * 2018-06-07 2018-11-02 北京唯公医疗技术有限公司 细胞分群方法
CN111999235A (zh) * 2020-07-03 2020-11-27 青岛农业大学 一种利用流式细胞术快速检测有活力的食用菌原生质体数量的方法

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US10900072B2 (en) 2021-01-26
AU2013280416B2 (en) 2017-06-15
JP6464081B2 (ja) 2019-02-06
AU2017228677A1 (en) 2017-10-05
US20160201122A1 (en) 2016-07-14
EP3828278A1 (en) 2021-06-02
CN108624654A (zh) 2018-10-09
JP2015522284A (ja) 2015-08-06
AU2017228677B2 (en) 2019-02-14
IN2015KN00073A (https=) 2015-07-31
KR20150036186A (ko) 2015-04-07
ZA201500239B (en) 2015-12-23
EP2867374A4 (en) 2016-05-11
US11767551B2 (en) 2023-09-26
BR112014032677A2 (pt) 2019-01-15
EP2867374A2 (en) 2015-05-06
RU2015102447A (ru) 2016-08-20
WO2014004609A3 (en) 2014-02-27
AU2013280416A1 (en) 2015-02-05
RU2671707C2 (ru) 2018-11-06
US20210139965A1 (en) 2021-05-13
EP2867374B1 (en) 2020-10-28
WO2014004609A2 (en) 2014-01-03
CA2877933A1 (en) 2014-01-03

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Application publication date: 20150527