CN104644284B - Small animal perfusion sampling fixing device and small animal perfusion sampling fixing method - Google Patents
Small animal perfusion sampling fixing device and small animal perfusion sampling fixing method Download PDFInfo
- Publication number
- CN104644284B CN104644284B CN201510087688.6A CN201510087688A CN104644284B CN 104644284 B CN104644284 B CN 104644284B CN 201510087688 A CN201510087688 A CN 201510087688A CN 104644284 B CN104644284 B CN 104644284B
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- perfusion
- operating platform
- fixing device
- small animal
- toy
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D7/00—Devices or methods for introducing solid, liquid, or gaseous remedies or other materials into or onto the bodies of animals
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D3/00—Appliances for supporting or fettering animals for operative purposes
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- Veterinary Medicine (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
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- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
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- Sampling And Sample Adjustment (AREA)
Abstract
The invention relates to the technical field of laboratory equipment, in particular to a small animal perfusion sampling fixing device and a small animal perfusion sampling fixing method. The device comprises a telescopic rod, a support vertical pillar and a base which are sequentially connected, wherein one side of the support vertical pillar is provided with mechanical arms and an operating platform, the operating platform is provided with limb fixing clamps and a heart fixing clamp, and the bottom of the operating platform is provided with a waste liquor collecting box. The small animal perfusion sampling fixing device has the beneficial effects that once the heart of a laboratory animal is punctured, the puncturing location is not likely to deviate, and positioning is relatively stable and accurate; by virtue of multi-azimuth adjusting, the laboratory animal is positioned on a relative position which ensures that an operator can perform test operation conveniently, and when the perfusion is performed, and the view of the operating platform is clean; experimental waste liquor is effectively collected, and environmental pollution is avoided; the multiple mechanical arms can be matched, so that perfusion of multiple laboratory animals can be realized at the same time; one man operation can be realized, and the manpower and time are saved; the perfusion technology can be rapidly mastered by experimenters, the waste of the laboratory animals is avoided, and the cost is saved.
Description
Technical field
The present invention relates to laboratory equlpment technical field, specifically a kind of toy perfusion is drawn materials fixing device and method.
Background technology
Laboratory animal perfusion draw materials be obtain good morphological observations and preserve brain, kidney, heart and other permitted
Multiple organ institute is required.Mainly comprise the following steps: after putting to death animal, cut rib rapidly, cut off diaphragm, expose heart, pipe of threading a needle
Pierce left ventricle, cut right atrium discharge opeing open, generally require the paraformaldehyde continuous perfusion 15 minutes using 4%, the time is more sometimes
Long.This operation requires experimenter to have preferably to start technical ability.Particularly with meiofauna such as mice, tire Mus, neonatal rat etc.
Perfusion difficulty big, have high demands, be embodied in: 1) mice has heart small volume, puncture needle easily passes left ventricle or wears
Broken interventricular septum leads to the failure；2) continuous perfusion whole process needs the hand-held piercing needle of experimenter, and the shake of handss is easily caused syringe needle and wears
Go out left ventricle or wear out interventricular septum failure；3) whole needs one people fixes puncture needle, injects perfusate for one, at least needs two people
Cooperation completes, and manpower and time are relatively costly.
Application No. " 201310061398.5 ", Authorization Notice No. are " cn 103070736 a ", entitled " toy is real
The Chinese invention patent of the perfusion fixing device tested and method " discloses a kind of perfusion fixing device of toy experiment and side
Method, including base, described base upper surface is provided with the first syringe fixed seat and the second syringe fixed seat, and is provided with first
Guide chute is corresponding with the first syringe fixed seat, is provided with that the second guide chute is corresponding with the second syringe fixed seat, and first leads
It is sliding combined with the first slide block in chute, be sliding combined with the second slide block in the second guide chute, set respectively in described base
There are the first motor and the second motor, described first motor passes through the first leading screw and nut mechanism and the first slide block connects
Connect, described second motor is connected with the second slide block by the second leading screw and nut mechanism, described first motor, second step
Stepper motor all electrically connects circuit for controlling motor, and base is provided with perfusion flow rate regulation button and motor control switch, described perfusion
Flow rate regulation button and motor control switch are all electrically connected with circuit for controlling motor.The defect of this patent of invention is: actual
Operating platform area is little, and the visual field is not good；The waste liquid that experiment produces can not be collected in time, pollute environment.
Content of the invention
It is an object of the invention to overcoming the deficiencies in the prior art, a kind of achievable one man operation being provided, has saved manpower
And the time, experimenter can grasp rapidly Perfusion, it is to avoid the waste of laboratory animal, saved a kind of toy of cost
Perfusion is drawn materials fixing device.
In order to achieve the above object, the fixing device the invention provides a kind of toy perfusion is drawn materials, including being sequentially connected
Expansion link, support post and base, wherein: the side of described support post is provided with mechanical arm and operating platform；
Described operating platform is provided with extremity fixation clamp and heart fixation clamp, and the bottom of this operating platform is provided with waste collection
Case.Puncture needle is used under the cooperation of extremity fixation clamp and heart fixation clamp is fixing, after to cardiac puncture, punctures position
Put and be not susceptible to offset.
Preferably, described operating platform is connected on support post by platform adjustment mechanism；
Described platform adjustment mechanism is provided with before and after's adjusting knob and left and right adjusting knob.By rotation before and after adjusting knob and
Left and right adjusting knob is adjusted to the position of platform, makes mice be in the relative position being easy to that operator carry out test operation
Put.
Preferably, cross bar is stretched out at the top of described expansion link, and the end of this cross bar is provided with hook.Hang on described hook
There is perfusate bag, perfusion fluid catheter is stretched out in the bottom of perfusate bag, this perfusion fluid catheter is provided with perfusate switch, described perfusion
Puncture needle connects the end of perfusion fluid catheter.The height of perfusate bag is adjusted by expansion link, to coordinate puncture.
Preferably, described support post is provided with up-down adjustment track, and this up-down adjustment track configures magnifier.Logical
Overregulate after up-down adjustment track makes magnifier be in proper height, the more concrete angle of magnifier is finely adjusted, until light
Source direction is radiated at mouse heart position；The optically focused that amplifies of magnifier acts on for the perfusion operation to mice especially tire Mus
It is more prone to.
Further, the side of described magnifier is provided with light source；
Described base is provided with the light source switch connecting with this light source.
Preferably, the number of described extremity fixation clamp is 4.4 extremity fixation clamps are used for gripping experiment respectively
The extremity of animal.
Preferably, described mechanical arm includes two sections of mutually hinged telescopic arms, and the free end of mechanical arm connects holds pin folder.
Described puncture needle is clipped in this and holds in pin folder, and puncture needle is fixed by mechanical arm, it is possible to achieve 360 ° of orientation rotations, is related to operating platform
The optional position of top, and under the fixation holding pin folder, positioning is more stable, accurate.
Preferably, described operating platform is connected by waste collection hole with waste liquid collecting box.Waste liquid collecting box can
The formaldehyde that overflows when collecting perfusion, it is to avoid environmental pollution.
A kind of toy perfusion is drawn materials fixing meanss, and the method comprises the steps:
The height of expansion link is adjusted；
Adjusting mechanical arm is at suitable position；
Perfusate bag is arranged on expansion link and by puncture needle retaining on the robotic arm, on adjustment operating platform, extremity are solid
The position of clamp and heart fixation clamp and angle；
Perfusion waste liquid flows in waste liquid collecting box through operating platform.
Perfusate in above-mentioned perfusate bag passes through puncture needle perfusion to toy heart.
Further, a kind of toy perfusion is drawn materials fixing meanss, and the method comprises the steps:
By the light source switch on lower bottom base, open light source；
The height of expansion link is adjusted, by adjusting knob before and after the rotation of platform adjustment mechanism and left and right adjusting rotation
Button is adjusted to the position of operating platform, and adjusting mechanical arm is at suitable position；
Perfusate bag is hung on the hook of cross bar, the pin of holding that perfusion puncture needle is fixed on mechanical arm presss from both sides, and perfusion is worn
Pricker is connected with perfusate bag by perfusion fluid catheter, and perfusate switch is in closure state；
The position of extremity fixation clamp and heart fixation clamp and angle on adjustment operating platform；
By adjusting after up-down adjustment track makes magnifier be in proper height, the more concrete angle of magnifier is carried out micro-
Adjust；
Observed from the magnifier visual field, open perfusate switch and be at opening, start perfusion；
Perfusion waste liquid can be flowed into the waste liquid collecting box of lower section through waste collection hole by operating platform, and continuous perfusion is to terminating.
Described toy can be the animals such as experiment mice, experimental rat, experimental rabbit.
The invention has the beneficial effects as follows, for puncture needle that laboratory animal heart is punctured in extremity fixation clamp and the heart
Used under the cooperation of dirty fixation clamp is fixing, after to cardiac puncture, puncture position is not susceptible to offset；Before rotation
Adjusting knob and left and right adjusting knob are adjusted to the position of platform afterwards, make mice be in be easy to operator carry out test behaviour
The relative position made；The height of perfusate bag can be adjusted by expansion link, to coordinate puncture；By adjusting up-down adjustment rail
After road makes magnifier be in proper height, the more concrete angle of magnifier is finely adjusted, until light source direction is radiated at mice
At cardiac position；The optically focused effect of amplifying of magnifier is more prone to for the operation of the perfusion of mice especially tire Mus；Puncture needle
Fixed by mechanical arm, it is possible to achieve 360 ° of orientation rotations, be related to the optional position above operating platform, and holding the fixation of pin folder
Under, positioning is more stable, accurate；Waste liquid collecting box can collect the formaldehyde overflowing during perfusion, it is to avoid environmental pollution；Can arrange in pairs or groups many
Mechanical arm, realizes perfusion while many laboratory animals.The present invention can achieve one man operation, has saved manpower and time, real
The personnel of testing can grasp rapidly Perfusion, it is to avoid the waste of laboratory animal, saved cost.
Brief description
Fig. 1 is the structural representation of the present invention；
Wherein:
1- expansion link 11- cross bar 12- links up with
13- perfusate bag 14- perfusion fluid catheter 15- perfusate switchs
2- support post 21- up-down adjustment track 22- magnifier
23- light source 231- light source switch 3- mechanical arm
31- holds pin folder 4- operating platform 41- extremity fixation clamp
42- heart fixation clamp 43- waste collection hole 44- waste liquid collecting box
Adjusting knob 52- left and right adjusting knob before and after 5- platform adjustment mechanism 51-
6- base 7- perfusion puncture needle
Specific embodiment
Below in conjunction with the drawings and specific embodiments, the present invention will be further described.
Embodiment 1:
A kind of toy perfusion as shown in Figure 1 is drawn materials fixing device, including the expansion link 1 being sequentially connected, support post 2
With base 6, the side of described support post 2 is provided with mechanical arm 3 and operating platform 4；
Described mechanical arm 3 includes two sections of mutually hinged telescopic arms, and the free end of mechanical arm 3 connects holds pin folder 31, wears
Pricker 7 is clipped in this and holds in pin folder 31.Puncture needle 7 is fixed by mechanical arm 3, it is possible to achieve 360 ° of orientation rotations, is related to operating platform 4
The optional position of top, and under the fixation holding pin folder 31, positioning is more stable, accurate.Using many mechanical arms, can achieve many
Perfusion while laboratory animal.
Described operating platform 4 is provided with four extremity fixation clamps 41 and heart fixation clamp 42.Puncture needle 7 is in extremity fixation clamp
41 and heart fixation clamp 42 cooperation fixing under used, after to cardiac puncture, position is not susceptible to offset.
The bottom of this operating platform 4 is provided with waste liquid collecting box 44, and operating platform 4 and waste liquid collecting box 44 pass through waste collection
Hole 43 is connected.Waste liquid collecting box 44 can collect the formaldehyde overflowing during perfusion, it is to avoid environmental pollution.
Described operating platform 4 is connected on support post 2 by platform adjustment mechanism 5, before and after platform regulation machine 5 is provided with
Adjusting knob 51 and left and right adjusting knob 52.
Cross bar 11 is stretched out at the top of described expansion link 1, and the end of this cross bar 11 is provided with hook 12.On described hook 12
Hang with perfusate bag 13, perfusion fluid catheter 14 is stretched out in the bottom of perfusate bag 13, and this perfusion fluid catheter 14 is provided with perfusate and opens
Close 15.Perfusion puncture needle 7 connects the end of perfusion fluid catheter 14.
Described support post 2 is provided with up-down adjustment track 21, and this up-down adjustment track 21 configures magnifier 22.Put
Big mirror 22 is more prone to for the perfusion operation of mice especially tire Mus.
The side of described magnifier 22 is provided with light source 23, and base 6 is provided with the light source switch connecting with this light source 23
231.
Actually used example:
Carry out perfusion from experiment mice to draw materials fixing experiment, method is as follows:
1st, press the light source switch 231 on lower bottom base 6, open light source 23；
2nd, the height of expansion link 1 is adjusted, by adjusting knob 51 and left and right before and after the rotation of platform adjustment mechanism 5
Adjusting knob 52 is adjusted to the position of operating platform 4, and adjusting mechanical arm 3 is at suitable position；
3rd, perfusate bag 13 is hung on the hook 12 of cross bar 11, the pin of holding that perfusion puncture needle 7 is fixed on mechanical arm 3 presss from both sides
On 31, perfusion puncture needle 7 is connected with perfusate bag 13 by perfusion fluid catheter 14, and perfusate switch 15 is in closure state；
4th, the mice anaesthetized is placed on operating platform 4, on adjustment operating platform 4, extremity fixation clamp 41 and heart are fixed
The position of folder 42 and angle, fix the extremity of mice respectively, so that mice is in and be easy to operator with four extremity fixation clamps 41
Carry out the relative position of test operation；
5th, cut off mice thoracic cavity and so that its heart is completely exposed, so that magnifier 22 is in by adjusting up-down adjustment track 21
After proper height, the more concrete angle of magnifier 22 is finely adjusted, until light source direction is radiated at mouse heart position, uses
Heart fixation clamp 42 is fixed to the cardiac position of mice；
6th, observed from magnifier 22 visual field, puncture needle 7 is pierced the left ventricle of mice by handheld mechanical arm 3, until
It can be seen that the blood back flow of beating is to pipeline；
7th, cut off right atrium, open perfusate switch 15 and be at opening, start perfusion.Flowed out by right atrium
Perfusate can be flowed into the waste liquid collecting box 44 of lower section through waste collection hole 43 by operating platform 4, and continuous perfusion is to terminating.
Below the preferred embodiment to the invention is illustrated, but the invention be not limited to described
Embodiment, those of ordinary skill in the art can also make without prejudice on the premise of the invention spirit a variety of etc.
Same modification or replacement, these equivalent modifications or replacement are all contained in the application claim limited range.
Claims (4)
1. a kind of toy perfusion draw materials fixing device it is characterised in that: include be sequentially connected expansion link (1), support post
(2) and base (6), wherein: the side of described support post (2) is provided with mechanical arm (3) and operating platform (4)；
Described mechanical arm (3) includes two sections of mutually hinged telescopic arms, and the free end of mechanical arm (3) connects holds pin folder (31)；
Described operating platform (4) is provided with extremity fixation clamp (41) and heart fixation clamp (42), and the bottom of this operating platform (4) sets
There is waste liquid collecting box (44)；
Described operating platform (4) is connected on support post (2) by platform adjustment mechanism (5)；
Described platform adjustment mechanism (5) is provided with before and after's adjusting knob (51) and left and right adjusting knob (52)；
Described support post (2) is provided with up-down adjustment track (21), and this up-down adjustment track (21) is upper to configure magnifier
(22)；
The side of described magnifier (22) is provided with light source (23)；
Described base (6) is provided with the light source switch (231) connecting with this light source (23).
2. a kind of toy perfusion according to claim 1 draw materials fixing device it is characterised in that: described expansion link
(1) cross bar (11) is stretched out at top, and the end of this cross bar (11) is provided with hook (12).
3. a kind of toy perfusion according to claim 1 draw materials fixing device it is characterised in that: described extremity are fixed
The number of folder (41) is 4.
4. a kind of toy perfusion according to claim 1 draw materials fixing device it is characterised in that: described operating platform
(4) connected by waste collection hole (43) with waste liquid collecting box (44).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510087688.6A CN104644284B (en) | 2015-02-26 | 2015-02-26 | Small animal perfusion sampling fixing device and small animal perfusion sampling fixing method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510087688.6A CN104644284B (en) | 2015-02-26 | 2015-02-26 | Small animal perfusion sampling fixing device and small animal perfusion sampling fixing method |
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| Publication Number | Publication Date |
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| CN104644284A CN104644284A (en) | 2015-05-27 |
| CN104644284B true CN104644284B (en) | 2017-01-18 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201510087688.6A Expired - Fee Related CN104644284B (en) | 2015-02-26 | 2015-02-26 | Small animal perfusion sampling fixing device and small animal perfusion sampling fixing method |
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Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106940333A (en) * | 2017-04-17 | 2017-07-11 | 山西医科大学 | The outer Electrophysiology research perfusion system of isolated cells |
| CN108514462A (en) * | 2018-06-11 | 2018-09-11 | 新乡医学院 | A kind of mouse perfusion fixing device and its perfusion fixing means |
| CN111700062B (en) * | 2020-06-19 | 2021-04-16 | 山东中医药大学 | Intelligent rodent heart perfusion device |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101953719A (en) * | 2010-08-20 | 2011-01-26 | 秦国秀 | Multifunctional experimental animal (rat) blood taking and dissection amplifier |
| CN102614030B (en) * | 2012-04-18 | 2014-12-17 | 新疆维吾尔自治区人民医院 | Device for fixing experimental animal during perfusion and application thereof |
| CN203425059U (en) * | 2013-07-17 | 2014-02-12 | 上海市同济医院 | Rat heart perfusion fixation device |
| CN203609541U (en) * | 2013-11-25 | 2014-05-28 | 成都大学 | Small animal whole-body tissue corrosion-prevention pouring system |
| CN203953871U (en) * | 2014-07-31 | 2014-11-26 | 王晓萃 | Experiment is poured into fixture with rat |
| CN204581591U (en) * | 2015-02-26 | 2015-08-26 | 中国人民解放军第二军医大学 | A kind of toy perfusion is drawn materials fixture |
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| CN104644284A (en) | 2015-05-27 |
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Granted publication date: 20170118 Termination date: 20180226 |