CN216365387U

CN216365387U - Laboratory is with supplementary blood sampling device of mouse model

Info

Publication number: CN216365387U
Application number: CN202122591933.0U
Authority: CN
Inventors: 李兰娟; 苏元帅; 褚清飞
Original assignee: First Affiliated Hospital of Zhejiang University School of Medicine
Current assignee: First Affiliated Hospital of Zhejiang University School of Medicine
Priority date: 2021-10-27
Filing date: 2021-10-27
Publication date: 2022-04-26
Anticipated expiration: 2031-10-27

Abstract

The utility model discloses a laboratory mouse model auxiliary blood sampling device, which comprises a body fixing part and a blood sampling frame; the body position fixing part comprises a body position fixing barrel which is of a cylindrical structure and is internally provided with a through cavity channel; the cover plate is movably covered on the upper port of the through cavity; a head fixing sleeve which is in a conical structure with a large upper part and a small lower part and is connected to the lower port of the through cavity; the pair of eye blood sampling holes are symmetrically formed in the head fixing sleeve; the clamping and fixing component is arranged on the body position fixing cylinder and is provided with a clamping end and an operating end; and the abdomen jacking component is arranged on the other side of the position fixing barrel opposite to the clamping and fixing component. The utility model has the advantages that the eye blood sampling operation of the mouse model is easily completed, the mouse model is prevented from shaking, the eye is accurately punctured, meanwhile, the direct contact between an operator and the mouse model is avoided, and the risk that the operator is bitten by the puncture needle when the operator fingers or the mouse model are punctured is effectively prevented.

Description

Laboratory is with supplementary blood sampling device of mouse model

Technical Field

The utility model relates to the technical field of animal model blood collection, in particular to a laboratory mouse model auxiliary blood collection device.

Background

In the field of biomedical basic research, animal models are generally required to be used for verifying experiments, and mouse models are the most commonly used experimental animal models; in the experiment, some experimental conditions are usually applied to a mouse model, and the influence of the experimental conditions on the living body is judged by observing the behavior change, the blood index change and the pathophysiological change in a tissue specimen. When the index change in the blood of the mouse model is judged, a blood sample needs to be reserved, and the volume of the mouse model is small, so that the blood content is small; in order to obtain more blood samples, the blood sampling methods adopted at present are: the blood sampling method comprises tail blood sampling (tail vein puncture, tail clipping blood sampling and the like), eye blood sampling and great vessel blood sampling (blood vessels such as femoral artery, carotid artery and the like), wherein the blood sampling by eye is large in volume, about a few milliliters, so the eye blood sampling is a common blood sampling method for mouse models.

Currently, when taking blood from an eye, an operator usually holds the mouse model with one hand to fix the posture (for example, the skin of the neck of the mouse is pinched between the thumb and the index finger of the left hand, and the abdomen of the mouse is grasped and squeezed by the middle finger, the ring finger and the little finger of the left hand), stands upside down to fill the eyeball with blood, punctures the eye of the mouse model with a blood taking needle by the right hand, and then connects the distal end of the blood taking needle to a blood taking tube to take blood. The eye blood sampling method is difficult to operate by a single person, the mouse model is easy to shake left and right when being held by a single hand, the eye is difficult to accurately puncture for blood sampling, and sometimes even the risk of finger puncture of an operator or bite injury by the mouse model occurs; in addition, due to the difficulty of one-hand operation, the mouse model can be inverted for too long time and the eyeball is congested, so that once the puncture is successful, blood can flow out in a splash shape and cannot be collected into the blood taking needle in time, and precious blood resources are lost and wasted.

Disclosure of Invention

The utility model aims to provide an auxiliary blood sampling device for a laboratory mouse model.

In order to achieve the purpose, the utility model can adopt the following technical scheme:

the utility model discloses a laboratory mouse model auxiliary blood sampling device, which comprises:

the integrated fixing part is used for fixing the body position of the mouse model and facilitating the blood sampling of eyes; and

the blood sampling frame is used for clamping and longitudinally supporting the body position fixing part;

wherein, the position fixed part includes:

the integrated fixed cylinder is of a cylindrical structure and is internally provided with a through cavity channel matched with the mouse model;

the cover plate is movably covered on the upper port of the through cavity;

a head fixing sleeve which is of a conical structure with a large upper part and a small lower part and is connected to the lower port of the through cavity;

the pair of eye blood sampling holes are symmetrically formed in the head fixing sleeve and used for penetrating blood sampling to sample blood of mouse model eyes;

the clamping and fixing assembly is arranged on the body position fixing barrel and is provided with a clamping end for clamping the skin of the neck of the mouse model and an operating end for driving the clamping end to draw the tail end of the mouse model; and

and the abdomen jacking component is arranged on the other side of the body position fixing barrel opposite to the clamping and fixing component and is used for repeatedly extruding the abdomen of the mouse model.

Furthermore, a pair of parallel rotating arms is arranged on the left side and the right side of the lower end of the body position fixing cylinder, and the head fixing sleeve is in clearance connection with the body position fixing cylinder through the rotating arms.

Furthermore, the head fixing sleeve is uniformly distributed with ventilating through holes.

Furthermore, the eye blood sampling hole is a strip-shaped hole formed along the height direction of the head fixing sleeve, a sliding block is arranged in the eye blood sampling hole in a sliding mode, and a positioning sleeve used for being matched with a puncture needle to penetrate into the center of the outer side face of the sliding block is vertically arranged in a penetrating mode.

Further, the clamping and fixing assembly comprises:

a clamp body;

the wire rewinding device is fixed on the inner wall of the body position fixing cylinder, a rotating wire rewinding rod of the wire rewinding device vertically penetrates out of the body position fixing cylinder, and a rotating handle is arranged at the outer end of the rotating wire rewinding rod;

one end of the pull wire is connected in the wire collector and is driven by the wire collector to collect and release wires, and the other end of the pull wire is connected with the clamp body.

Furthermore, a clamping operation hole for the clamp body to pass through is formed in the head fixing sleeve.

Further, the abdomen pressing component comprises:

the pressing rod vertically penetrates through the cylinder wall of the body position fixing cylinder;

the abdominal wall pressing plate is vertically and fixedly connected to the inner end of the pressing rod and is used for pressing the abdomen of the mouse model;

the operating plate is arranged at the outer end of the pressure rod and used for driving the pressure rod to push the abdominal wall pressure plate inwards; and

the top supporting spring is sleeved on the pressure rod between the body position fixing cylinder and the operating plate;

the operation plate is an inclined plate obliquely hinged to the upper end of the pressure rod, and the oblique lower end of the operation plate is hinged to the outer wall of the body position fixing barrel.

Further, the blood collection holder includes:

a horizontal support;

the vertical supporting rod is vertically and fixedly connected to the horizontal supporting seat;

the horizontal supporting rod is vertically and fixedly connected to the vertical supporting rod; and

and the sleeve clamping component is fixedly connected on the horizontal supporting rod and used for clamping the body position fixing cylinder so as to enable the body position fixing cylinder to be longitudinally suspended above the horizontal supporting seat.

Further, the sleeve clamping assembly includes:

the C-shaped clamp plate is horizontally and fixedly connected with the outer end of the horizontal supporting rod;

the arc-shaped top pressure plate is matched and attached to the inner side wall of one side of the opening of the C-shaped clamping plate;

the jacking screw is vertically screwed on and penetrates through the C-shaped clamping plate, the inner end of the jacking screw is fixedly connected with the center of the arc-shaped jacking plate, and the arc-shaped jacking plate is driven to move inwards to clamp the position fixing part arranged in the C-shaped clamping plate; and

and the operating handle is fixedly connected with the outer end of the jacking screw rod.

Furthermore, be provided with one on the top surface of horizontal support just to heparin tube fixing base of sleeve clamping subassembly below, a pair of heparin tube slot has been seted up on the heparin tube fixing base side by side.

The utility model has the advantages that the body position fixing part designed by utilizing the rat drilling habit can ensure that a mouse model can be easily drilled into the body position fixing cylinder, the head fixing sleeve with a conical structure is matched with one end of the body position fixing cylinder, and the clamping and fixing assembly is arranged on the body position fixing cylinder, so that the head of the mouse model can be stably clamped in the head fixing sleeve, and the eye of the mouse model just faces an eye blood sampling hole formed in the head fixing sleeve, thereby easily completing the eye blood sampling operation of the mouse model, preventing the mouse model from shaking, ensuring accurate eye puncture, avoiding direct contact between an operator and the mouse model, and effectively preventing the risk that the operator is bitten by the puncture needle for puncturing the fingers of the operator or the mouse model; meanwhile, the abdomen jacking assembly arranged on the body position fixing barrel can repeatedly squeeze the abdomen of the mouse model in the blood sampling process, so that blood flow is promoted, and the blood sampling amount is guaranteed to the maximum extent; in addition, with the supporting blood sampling frame that uses of position fixed part can also vertically prop up solid position fixed part, make mouse model in the position fixed part take a blood sample of head down, utilize the light messenger's eye blood of the action of gravity of blood to flow into in the blood sampling pipe, reduce the blood sample of blood sampling in-process and lose, liberation operating personnel both hands, light realization one-man blood sampling operation.

Drawings

Fig. 1 is a schematic structural view of the present invention.

Fig. 2 is an enlarged view of the body part fixing portion of fig. 1.

FIG. 3 is a top view of the blood collection holder of FIG. 1.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

It should be noted that all the directional indicators (such as up, down, left, right, front, and rear … …) in the embodiment of the present invention are only used to explain the relative position relationship between the components, the movement situation, etc. in a specific posture (as shown in the drawing), and if the specific posture is changed, the directional indicator is changed accordingly.

As shown in fig. 1, the laboratory mouse model auxiliary blood sampling device of the present invention includes a body position fixing portion and a blood sampling frame used in cooperation with the body position fixing portion; wherein, this position fixed part is used for fixed mouse model position, is convenient for carry out the eye blood sampling to mouse model, and this blood sampling frame is used for the centre gripping and vertically props up solid position fixed part.

Specifically, as shown in fig. 2, the body position fixing portion includes a body position fixing cylinder 1, a cover plate 2, a head fixing sleeve 3, a pair of eye blood sampling holes 4, a clamping fixing component 5 and an abdomen pressing component 6.

The body position fixing barrel 1 is of a cylindrical structure, a through cavity matched with a mouse model is formed in the body position fixing barrel 1, the body position fixing barrel 1 of various models can be designed according to mouse models of different sizes, and therefore the body position fixing barrel 1 of the corresponding model can be selected according to the size of the mouse model to conduct eye blood sampling operation.

The cover plate 2 is movably covered on the upper port of the through cavity of the body position fixing barrel 1 in a sealing manner, the cover plate 2 can be directly buckled on the body position fixing barrel 1 in a sealing manner, and the most preferable manner is to hinge the cover plate 2 on the upper port of the body position fixing barrel 1 in a hinging manner.

The head fixing sleeve 3 is connected to the lower port of the through cavity of the body position fixing cylinder 1, and the shape of the head fixing sleeve is a conical structure with a large upper part and a small lower part, so that the head fixing sleeve can be just matched with the shape of the head of a mouse model. Specifically, be provided with a pair of rotor arm 7 that are parallel to each other in the lower extreme left and right sides of position fixed cylinder 1, the fixed cover of head 3 passes through

rotor arm

7 and 1 clearance connection of position fixed cylinder to make the fixed cover of head 3 can rotate as required level, and then accomplish and carry out the rotation adjustment about boring the mouse model head in the fixed cover of head 3. In addition, the head fixing sleeve 3 can be uniformly distributed with ventilation through holes 8 to ensure that the mouse model can breathe smoothly.

The two eye blood sampling holes 4 are symmetrically arranged on the head fixing sleeve 3 and are used for penetrating blood sampling to perform puncture blood sampling on the eyes of the mouse model. Specifically, the positions of the two eye blood sampling holes 4 correspond to the positions of the two eyes of the mouse model drilled into the head fixing sleeve 3, and the two eye blood sampling holes 4 are respectively positioned on the left side and the right side of the rotation direction of the two rotating arms 7, so that the eye blood sampling hole 4 on one side can be vertically downward by adjusting the rotation position of the head fixing sleeve 3. In addition, the two eye blood sampling holes 4 are strip-shaped holes which are arranged along the height direction of the head fixing sleeve 3, a sliding block 9 is arranged in each eye blood sampling hole 4 in a sliding mode, and a positioning sleeve 10 which is used for being matched with a puncture needle to penetrate into the center of the outer side face of each sliding block 9 vertically penetrates through the center of the outer side face of each eye blood sampling hole.

The clamping and fixing component 5 is arranged on the body position fixing barrel 1 and is provided with a clamping end for clamping the skin of the neck and the neck of the mouse model and an operating end for driving the clamping end to draw the tail end of the mouse model. Specifically, the clamping and fixing component 5 comprises a clamp body 5.1, a wire rewinding device 5.2 and a pull wire 5.3; the wire rewinding device 5.2 is fixed on the inner wall of the body position fixing barrel 1 and is provided with a rotary wire rewinding rod 5.4 capable of rotating to complete wire rewinding and unwinding, the rotary wire rewinding rod 5.4 vertically penetrates out of the body position fixing barrel 1, and a rotary handle 5.5 convenient for an operator to grasp and operate can be arranged at the outer end of the rotary wire rewinding rod 5.4; one end of the pull wire 5.3 is connected in the wire-rewinding device 5.2, the wire-rewinding device 5.2 drives the wire to rewind and pay, and the other end is connected with the clamp 5.1. In addition, in order to facilitate the operation of clamping the neck skin of the clamp 5.1 after the mouse model is drilled into the head fixing sleeve 3, a clamping operation hole 11 for the clamp 5.1 to pass through is formed in the head fixing sleeve 3 corresponding to the neck skin of the mouse model, so that the clamp 5.1 can be firstly paid off to penetrate out from the clamping operation hole 11 before the mouse model is drilled, and then the clamp 5.1 is operated outside after the mouse model is drilled into the head fixing sleeve 3, so that the clamp 5.1 can extend into the head fixing sleeve 3 to easily clamp the neck skin of the mouse model.

The abdomen jacking component 6 is arranged on the other side of the body position fixing barrel 1 opposite to the clamping fixing component 5 and used for repeatedly extruding the abdomen of the mouse model, so that blood flow is promoted in the blood sampling process, and the blood sampling amount is guaranteed to the maximum extent. Specifically, the abdomen jacking component 6 comprises a pressure lever 6.1, an abdominal wall pressure plate 6.2, an operation plate 6.3 and a jacking spring 6.4; the pressing rod 6.1 vertically penetrates through the wall of the body position fixing barrel 1, the abdominal wall pressing plate 6.2 is vertically and fixedly connected to the inner end of the pressing rod 6.1 and used for pressing the abdomen of a mouse model, the operating plate 6.3 is arranged at the outer end of the pressing rod 6.1 and used for driving the pressing rod 6.1 to inwards push the abdominal wall pressing plate 6.2, and the jacking spring 6.4 is sleeved on the pressing rod 6.1 between the body position fixing barrel 1 and the operating plate 6.3, wherein the operating plate 6.3 is an inclined plate obliquely hinged to the upper end of the pressing rod 6.1, and the oblique lower end of the operating plate 6.3 is hinged to the outer wall of the body position fixing barrel 1.

As shown in fig. 3, the blood collecting rack comprises a horizontal support 12, a vertical support rod 13, a horizontal support rod 14 and a set of collet fixing components 15; wherein, the upper surface of horizontal support 12 has a horizontal bearing surface, and vertical branch 13 links firmly on horizontal support 12 perpendicularly, and horizontal branch 14 links firmly on vertical branch 13 perpendicularly, and sleeve clamping subassembly 15 links firmly on horizontal branch 14 for the fixed section of thick bamboo 1 of centre gripping position makes its vertical suspension in horizontal support 12 top. Specifically, the sleeve clamping component 15 comprises a C-shaped clamping plate 15.1, an arc-shaped top pressing plate 15.2, a top pressing screw 15.3 and an operating handle 15.4; the C-shaped clamping plate 15.1 is horizontally fixedly connected to the outer end of the horizontal supporting rod 14, the arc-shaped jacking plate 15.2 is suitable for being attached to the inner side wall of one side of the opening of the C-shaped clamping plate 15.1 in a matching mode, the jacking screw 15.3 is vertically screwed on the C-shaped clamping plate 15.1 in a penetrating mode, and the inner end of the arc-shaped jacking plate is fixedly connected with the center of the arc-shaped jacking plate 15.2, so that the arc-shaped jacking plate 15.2 can be driven to move inwards by screwing the jacking screw 15.3, a body position fixing portion in the C-shaped clamping plate 15.1 is clamped, the operating handle 15.4 is fixedly connected to the outer end of the jacking screw 15.3, and an operator can conveniently manually screw the jacking screw 15.3 in a screwing mode. In addition, in order to facilitate the blood collection of the mouse model when the body position fixing part is clamped on the sleeve clamping component 15, a blood collection tube for receiving blood can be just placed under the body position fixing part, a blood collection tube fixing seat 16 which is just opposite to the lower part of the sleeve clamping component 15 is further arranged on the top surface of the horizontal support 12, and a pair of blood collection tube slots 17 are arranged on the blood collection tube fixing seat 16 side by side.

When in use, firstly the wire is wound by the wire winding device 5.2, so that the clamp body 5.1 penetrates out of the clamping operation hole 11 on the head fixing sleeve 3; then, a position fixing part is transversely arranged, a mouse model is drilled into a through cavity channel from the end, connected with a cover plate 2, of a position fixing cylinder 1, the mouse model can climb to a head fixing sleeve 3 by utilizing the drilling habit of rats, at the moment, cotton wool and other articles are filled into the through cavity channel, and the cover plate 2 is buckled, so that the way of the mouse model is blocked; then the clamp body 5.1 is operated to clamp the neck skin of the mouse model, and a wire is wound up through a wire winder 5.2 to draw the neck of the mouse model, so that the eyes of the mouse model are right opposite to the eye blood collecting holes 4 on the head fixing sleeve 3; then, the body position fixing cylinder 1 is longitudinally arranged, the head fixing sleeve 3 faces downwards (namely, a mouse model is inverted), the head fixing sleeve is inserted into a C-shaped clamping plate 15.1 of the blood sampling frame, the jacking screw 15.3 is rotated to enable an arc-shaped jacking plate 15.2 to jack the outer wall of the body position fixing cylinder 1, and the body position fixing cylinder 1 is clamped in the C-shaped clamping plate 15.1; then, a blood collection tube is placed in a blood collection tube slot 17 on the blood collection tube fixing seat 16, and the head fixing sleeve 3 is rotated according to the position of the blood collection tube, so that the eyes on one side of the mouse model are opposite to the blood collection tube below the mouse model; then, sliding the slide block 9 on the eye blood sampling hole 4 to ensure that the slide block is opposite to the mouse model eye, and at the moment, ensuring that the mouse model eye, the positioning sleeve 10 on the slide block 9 and the blood sampling tube are positioned on the same axis; then, the blood sampling puncture needle can be operated to be inserted into the positioning sleeve 10, so that the eyes of the mouse model are punctured, the eye blood flows into the blood sampling tube along the positioning sleeve 10, and the eye blood sampling is completed; when blood is collected from the eyes of the mouse model, an operator can manually and repeatedly press the operating plate 6.3 to repeatedly press the abdominal wall pressing plate 6.2 to repeatedly press the abdomen of the mouse model, so that blood flow is promoted in the blood collecting process, and the blood collecting amount is guaranteed to the maximum extent; after blood of eyes at one side of the mouse model is collected, the head fixing sleeve 3 can be rotated to collect blood of eyes at the other side.

Claims

1. The utility model provides a laboratory is with supplementary blood sampling device of mouse model which characterized in that includes:

wherein, the position fixed part includes:

the cover plate is movably covered on the upper port of the through cavity;

2. The laboratory mouse model assisted blood sampling device according to claim 1, wherein a pair of parallel rotating arms are arranged on the left and right sides of the lower end of the body position fixing cylinder, and the head fixing sleeve is in clearance connection with the body position fixing cylinder through the rotating arms.

3. The laboratory mouse model assisted blood sampling device as claimed in claim 1, wherein the head fixing sleeve is uniformly provided with air-permeable through holes.

4. The laboratory mouse model assisted blood sampling device according to claim 1, wherein the eye blood sampling hole is a strip-shaped hole formed along the height direction of the head fixing sleeve, a sliding block is slidably arranged in the eye blood sampling hole, and a positioning sleeve adapted to penetrate a puncture needle is vertically arranged at the center of the outer side surface of the sliding block in a penetrating manner.

5. The laboratory mouse model assisted lancing device of claim 1, wherein the clamping fixture assembly comprises:

a clamp body;

6. The laboratory mouse model assisted blood sampling device as claimed in claim 5, wherein a clamping operation hole for the clamp body to pass through is formed on the head fixing sleeve.

7. The laboratory mouse model assisted blood collection device of claim 1, wherein the abdominal compression assembly comprises:

8. The laboratory mouse model assisted lancing device of claim 1, wherein the lancing rack comprises:

a horizontal support;

9. The laboratory mouse model assisted blood sampling device of claim 8, wherein the sleeve holder assembly comprises:

10. The laboratory mouse model assisted blood sampling device according to claim 8 or 9, wherein a blood sampling tube fixing seat is disposed on the top surface of the horizontal support and aligned with the lower portion of the sleeve clamping assembly, and a pair of blood sampling tube insertion grooves are formed in the blood sampling tube fixing seat side by side.