CN111700062B - Intelligent rodent heart perfusion device - Google Patents
Intelligent rodent heart perfusion device Download PDFInfo
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- CN111700062B CN111700062B CN202010563158.5A CN202010563158A CN111700062B CN 111700062 B CN111700062 B CN 111700062B CN 202010563158 A CN202010563158 A CN 202010563158A CN 111700062 B CN111700062 B CN 111700062B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0236—Mechanical aspects
- A01N1/0242—Apparatuses, i.e. devices used in the process of preservation of living parts, such as pumps, refrigeration devices or any other devices featuring moving parts and/or temperature controlling components
- A01N1/0247—Apparatuses, i.e. devices used in the process of preservation of living parts, such as pumps, refrigeration devices or any other devices featuring moving parts and/or temperature controlling components for perfusion, i.e. for circulating fluid through organs, blood vessels or other living parts
Abstract
The utility model provides an intelligence rodent heart perfusion device, relates to animal experiment device technical field, including perfusion chamber, dissection table and intelligent perfusion ware, be equipped with stock solution storehouse, transport storehouse, control storehouse in the intelligence perfusion ware, dissection table on be equipped with the animal fixer for fixed laboratory animal, be equipped with drawing liquid pump B in the transport storehouse, the liquid input of drawing liquid pump B and the liquid intercommunication in the stock solution storehouse, liquid output end has heart fixing container through liquid delivery hose connection, still is equipped with electromagnetic blood flow sensor in the heart fixing container, is equipped with the controller in the control storehouse, electromagnetic blood flow sensor passes through the signal line and is connected with the controller, the controller passes through the electric wire and is connected with drawing liquid pump B, controller still electric connection has the power, intelligent perfusion ware uses with the cooperation of dissection table, the dissection table sets up in the perfusion chamber. The invention has simple operation and convenient use, can realize the intelligent operation of multi-channel perfusion, reduces the labor intensity of experiment operators, improves the perfusion efficiency and ensures the perfusion quality.
Description
Technical Field
The invention relates to the technical field of animal experiment devices, in particular to an intelligent rodent heart perfusion device.
Background
In animal experiments, heart perfusion material drawing is an essential step, and perfusion results are closely related to the state of tissues. The heart perfusion is to expose the animal heart, insert the perfusion needle into the left ventricle, fix the perfusion needle, and perfuse the normal saline, pbs and paraformaldehyde sequentially. At present, no special animal heart perfusion device exists in the market, most experimenters adopt a flat-mouth needle head and a hose which are assembled by self, and the needle head is inserted into the heart and then clamps and closes the cardiac apex and the perfusion needle by using vascular clamps.
The traditional self-assembled perfusion device has the following defects:
1. the perfusion device and the surgical instrument are scattered, the assembly procedure is complex, and the preparation needs a long time before the operation;
2. must rely on the vascular pincers to fix, to the littleer small-size animal of heart, like mouse, young rat etc. usually need many times to try, can press from both sides tightly fixedly, not only the time-wasting, and the animal state also receives the influence, accurately pierces the left ventricle, pierces the depth and also hardly guarantees, and the perfusion success rate is lower.
3. The vascular clamp needs to clamp the apex of the heart and the perfusion needle, and the heart is easily damaged by slight struggle or shake of the animal, so that perfusion failure is caused.
4. The perfusion needs to use paraformaldehyde irritability great, and traditional flowing device does not have effective exhaust and recovery unit, and is very big to the human body injury.
5. The conventional perfusion apparatus lacks a corresponding dissecting table 20, and animal fixation and dissecting operations are complicated.
6. At present, a heart mould without a perfusion device is lack of a mould integrating fixation and automatic penetration, so that the experimental efficiency is low and the success rate of 100 percent cannot be guaranteed.
7. The traditional perfusion device judges the perfusion state only by observing the tissue color through naked eyes, cannot accurately master the state of a perfusion animal, is difficult to ensure the accuracy of an experimental result, and wastes buffer solution and fixing solution.
8. The traditional perfusion device still cannot effectively perform multi-channel experiments by using a peristaltic pump, the operation flow is complex, and the experiment efficiency is low.
Disclosure of Invention
To solve the problems of the prior art, the present invention provides an intelligent rodent heart perfusion device which systematically improves the disadvantages of the prior art.
In order to achieve the purpose, the technical scheme of the invention is as follows:
the utility model provides an intelligence rodent heart perfusion device, includes perfusion chamber, dissection table and intelligent perfusion ware, intelligent perfusion ware include the casing, the casing in be equipped with stock solution storehouse, transport storehouse, control bin, the dissection table on be equipped with the animal fixer that is used for fixed laboratory animal, the transport storehouse in be equipped with drawing liquid pump B, the liquid input of drawing liquid pump B and the liquid intercommunication in the stock solution storehouse, liquid output end has the heart fixed container through liquid delivery hose connection, liquid delivery hose's end connection have the perfusion needle, the point thorn end of perfusion needle pierce through the outer wall of heart fixed container and extend to the heart fixed container inside, the heart fixed container in still be equipped with electromagnetic blood flow sensor, the control bin in be equipped with the controller, electromagnetic blood flow sensor pass through the signal line and be connected with the controller, the controller is connected with the liquid pump B through an electric wire, the controller is also electrically connected with a power supply, the intelligent perfusion device is matched with an anatomical table for use, and the anatomical table is arranged in the perfusion chamber.
Preferably, a washing bottle for containing cleaning liquid, a buffer bottle for containing buffer liquid and a fixing bottle for containing fixing liquid are arranged in the liquid storage bin; the transfer bin is internally provided with 1 liquid pump A and a plurality of liquid pumps B, the bottoms of the flushing bottle, the buffer bottle and the fixed bottle are all provided with liquid outlets, the washing bottle is provided with 1 liquid outlet and is connected with the liquid input end of the liquid pump A through a liquid conveying pipe, the liquid output end of the liquid pump A is connected with a three-way joint, one end of the three-way joint is connected with the liquid pump A, the other two ends are respectively communicated with the buffer bottle and the fixed bottle through liquid conveying pipes which run through the top ends of the buffer bottle and the fixed bottle, the bottom of buffer bottle, fixed bottle be equipped with a plurality of liquid outlets, the liquid outlet of buffer bottle or fixed bottle be connected with the liquid input of drawing liquid pump B of difference through the liquid conveying pipe respectively, arbitrary the liquid outlet in all be equipped with the solenoid valve, the controller respectively with solenoid valve, drawing liquid pump A electric connection.
Preferably, the control cabin is further provided with a touch screen, a display opening of the touch screen is arranged on the front end face of the shell where the control cabin is located, a power switch button is further arranged on the front end face, and the controller is connected with the touch screen through an electric wire and a signal wire.
Preferably, the touch screen is further provided with an experiment control unit, the experiment control unit classifies operation modes by subdividing according to different types, strains, ages in days and weights of experimental animals, the operation modes are set to specific perfusion time and perfusion speed, and the controller controls the electromagnetic valve, the liquid drawing pump a and the liquid drawing pump B to implement the operation modes, and the acquisition modes of the perfusion time and the perfusion speed are as follows: selecting perfusion time and perfusion speed which can obtain optimal perfusion effect as perfusion operation modes of any small animal according to the result of multiple perfusion experiments of the small animal subdivided into the same subclass and data of relevant perfusion time and perfusion speed, wherein the experiment control unit displays a control interface on a touch screen, the control interface comprises a first interface and a second interface, the first interface is provided with type selection, strain selection, age-per-day input, weight input, a cleaning button and a perfusion button, the perfusion button is touched to enter the second interface, the second interface is provided with a channel selection button, and a start button, a pause button and a stop button are arranged above the channel selection button; the channel selection button is used for selecting perfusion operation of animal hearts in different heart fixing containers, and the controller controls different liquid pumps B and electromagnetic valves matched with the liquid pumps B to perfuse.
Preferably, the heart fixing container is of a shell-like structure, the shell-like structure comprises a shell cover A and a shell cover B which are hinged with each other, inner cavities matched with the placing shapes of the hearts of the animals are arranged in the shell cover A and the shell cover B, clamping pieces A matched with each other are respectively arranged at the edges of the shell cover A and the shell cover B and are connected in a clamping mode through the clamping pieces A, and overflow ports are also arranged at the edges of the shell cover A and the shell cover B; the puncture needle of the electromagnetic blood flow sensor is positioned in the inner mold cavity, the puncture needle, the perfusion needle and the inner mold cavity are matched with the animal heart placed in the inner mold cavity, when the animal heart is placed in the inner mold cavity and the heart fixing container is closed, the puncture needle of the electromagnetic blood flow sensor punctures the pericardium, and the perfusion needle punctures the left ventricle; the heart fixing containers are multiple, and inner mold cavities with different shapes and sizes are arranged to meet the fixing requirements of different animal hearts.
Preferably, the animal fixer set up in the middle part of dissection table, including the recess that matches with the animal size and thorax press from both sides, the thorax press from both sides one end and the cell wall top of one side of recess articulated, the other end passes through fastener B and is connected with the cell wall top buckle of recess opposite side, is equipped with the operation mouth in the middle part of thorax press from both sides.
Preferably, the table-board of the dissection table at the periphery place of recess on still be equipped with the drainage groove, the drainage groove surround in the recess, and the tank bottom of drainage groove is to the gradual downward sloping extension of one end to be equipped with the drainage tube in the lowest department of tank bottom, the drainage tube be connected with waste liquid collection container through the connecting pipe.
Preferably, the top of perfusion chamber be equipped with transparent cage, be equipped with the light at the lower surface of cage, be equipped with the blow vent on the lateral wall of the both sides of perfusion chamber respectively, the outer wall of the one end of perfusion chamber on still be equipped with the gas vent, install the air discharge fan in gas vent department, the gas vent be located the outdoor one end of perfusion chamber and be connected with the formaldehyde purification ware through the breather pipe, the atmosphere is discharged to the gas after purifying.
Preferably, the connecting pipe is connected with a waste liquid collecting container through a waste liquid conveying hose, and the waste liquid collecting container is arranged outside the perfusion chamber; and a liquid leakage hole is formed at the bottom of the groove and is connected with the connecting pipe through a connecting pipe.
Preferably, the controller is a control chip or a control circuit board or a PLC controller.
The intelligent rodent heart perfusion device has the following beneficial effects:
1. the perfusion device is simple to operate and convenient to use, and perfusion can be easily performed at the optimal perfusion time and perfusion speed through the interface button on the touch screen, so that the labor intensity of an experiment operator is greatly improved, the perfusion efficiency is improved, and the experiment quality is ensured;
2. the invention realizes the unified control of the multi-channel perfusion operation and can perform the perfusion operation on a plurality of animal hearts at the same time;
3. the heart fixing container is provided with the inner cavity adaptive to the animal heart, so that the puncture needle and the perfusion needle of the electromagnetic blood flow sensor can be respectively punctured into corresponding parts of the heart while the heart fixing container is closed, the difficulty of heart fixing operation is greatly reduced, and the experimental efficiency and the success rate are improved;
4. by arranging the drainage groove, the drainage tube, the leakage hole, the connecting tube and the waste liquid collecting container, residual liquid of blood, buffer solution, flushing fluid and stationary liquid in the experimental process can be smoothly collected into the waste liquid collecting container, so that the cleanness and the sanitation of the dissecting table are facilitated;
5. the invention can quickly discharge the air containing formaldehyde in the perfusion chamber by arranging the exhaust fan, the vent pipe and the formaldehyde purifier, and the air is discharged into the atmosphere after being purified, thereby ensuring the environmental protection and protecting the health of experiment operators.
6. The optimal perfusion time and the optimal perfusion speed related by the invention are obtained based on a large number of perfusion experiments of animals of the same subclass, and the optimal perfusion time and the optimal perfusion speed are arranged in the perfusion process of the intelligent perfusion device through the controller, so that perfusion can be carried out in an optimal perfusion mode under the simplest operation.
Drawings
FIG. 1: the invention discloses a schematic view of a top-down structure of an intelligent perfusion device;
FIG. 2: the invention discloses a schematic side view structure diagram of an intelligent perfusion device;
FIG. 3: the invention discloses a front view structure schematic diagram of an intelligent perfusion device;
FIG. 4: the invention discloses a top view of the matching relationship between a perfusion chamber and an dissecting table;
FIG. 5: the invention discloses a structural schematic diagram of a heart fixing container;
FIG. 6: a first interface schematic of the present invention;
FIG. 7: a second interface schematic of the invention;
1: housing, 1-1: a liquid storage bin, 1-2: a transfer bin, 1-3: control cabin, 2: touch screen, 3: power switch button, 4: rinse bottle, 5: buffer bottle, 6: fixed bottle, 7: liquid pump a, 8: liquid pump B, 9: liquid delivery pipe, 10: three-way connection, 11: heart fixation vessel, 11-1: case cover A, 11-2: case cover B, 11-3: inner cavity, 11-4: electromagnetic blood flow sensor, 11-5: perfusion needle, 11-6: an overflow port, 11-7: a hinged shaft, 11-8: fastener a, 12: liquid outlet, 13: controller, 14: signal line, 15: electric wire, 16: delivery pipe, 17: cage, 18: vent, 19: illumination lamp, 20: dissecting table, 20-1: groove, 20-2: chest clip, 20-3: drainage groove, 20-4: fastener B, 20-5: drainage tube, 20-6: surgical operation port, 21: connecting pipe, 22: waste liquid conveying hose, 23: waste liquid collection container, 24: breather pipe, 25: formaldehyde purifier, 26: an exhaust fan.
Detailed Description
In the following, embodiments of the present invention are described in detail in a stepwise manner, which is merely a preferred embodiment of the present invention and is not intended to limit the scope of the present invention, and any modifications, equivalents, improvements, etc. made within the spirit and principle of the present invention should be included in the scope of the present invention.
In the description of the present invention, it should be noted that the terms "upper", "lower", "left", "right", "top", "bottom", "inner", "outer", and the like indicate orientations and positional relationships based on the orientations and positional relationships shown in the drawings, and are only used for describing the present invention and simplifying the description, but do not indicate or imply that the referred device or element must have a specific orientation and a specific orientation configuration and operation, and thus, the present invention is not to be construed as being limited thereto.
Referring to FIGS. 1 to 7:
in one embodiment, an intelligent rodent heart perfusion device is shown in fig. 1-3 and comprises a perfusion chamber, an dissecting table 20 and an intelligent perfusion device, wherein the intelligent perfusion device comprises a housing 1, a liquid storage bin 1-1, a transfer bin 1-2 and a control bin 1-3 are arranged in the housing 1, an animal fixer for fixing an experimental animal is arranged on the dissecting table 20, a liquid pump B8 is arranged in the transfer bin 1-2, a liquid input end of the liquid pump B8 is communicated with liquid in the liquid storage bin 1-1, a liquid output end is connected with a heart fixing container 11 through a liquid conveying hose 9, as shown in fig. 5, an end of the liquid conveying hose 9 is connected with a perfusion needle 11-5, a sharp piercing end of the perfusion needle 11-5 penetrates through the outer wall of the heart fixing container 11 and extends into the heart fixing container 11, the heart fixing container 11 is further internally provided with an electromagnetic blood flow sensor 11-4, as shown in fig. 2, a controller 13 is arranged in the control bin 1-3, the electromagnetic blood flow sensor 11-4 is connected with the controller through a signal line 14, the controller 13 is connected with a liquid pump B8 through an electric wire, the controller 13 is further electrically connected with a power supply, the intelligent perfusion device is used in cooperation with the dissecting table 20, as shown in fig. 4, the dissecting table 20 is arranged in the perfusion chamber.
In a further embodiment, as shown in fig. 1, a washing bottle 4 for containing a cleaning solution, a buffer bottle 5 for containing a buffer solution, and a fixing bottle 6 for containing a fixing solution are arranged in the liquid storage bin 1-1; the transfer bin 1-2 is internally provided with 1 liquid pump A7 and a plurality of liquid pumps B8, the bottoms of the flushing bottle 4, the buffer bottle 5 and the fixed bottle 6 are respectively provided with a liquid outlet 12, the flushing bottle 4 is provided with 1 liquid outlet 12 and is connected with the liquid input end of the liquid pump A7 through a liquid conveying pipe 9, the liquid output end of the liquid pump A7 is connected with a three-way joint, one end of the three-way joint is connected with the liquid pump A7, the other two ends are respectively communicated with the buffer bottle 5 and the fixed bottle 6 through the liquid conveying pipe 9 penetrating through the top ends of the buffer bottle 5 and the fixed bottle 6, the bottoms of the buffer bottle 5 and the fixed bottle 6 are provided with a plurality of liquid outlets 12, the liquid outlets 12 of the buffer bottle 5 or the fixed bottle 6 are respectively connected with the liquid input ends of different liquid pumps B8 through the liquid conveying pipe 9, any one of the liquid outlets 12 is internally provided with an electromagnetic valve (not shown in the figure, the controller 13 is respectively electrically connected with the electromagnetic valve and the liquid pump A; in order to facilitate the maintenance and the replacement of liquid, door opening and closing structures can be arranged at the tops of the liquid storage bin 1-1 and the transfer bin 1-2.
In a further embodiment, as shown in fig. 2 and fig. 3, the control cabin 1-3 is further provided with a touch screen 2, a display opening of the touch screen 2 is located on a front end face of the housing 1 where the control cabin 1-3 is located, the front end face is further provided with a power switch button 3, and the controller 13 is connected with the touch screen 2 through an electric wire and a signal wire.
In a further embodiment, as shown in fig. 6 and 7, the touch panel 2 is further provided with an experiment control unit, the experiment control unit classifies operation modes by subdividing according to the type, strain, age and weight of the experimental animal, the operation modes are set as specific perfusion time and perfusion speed, and the controller 13 controls the electromagnetic valve, the liquid drawing pump a and the liquid drawing pump B to implement the operation modes, and the acquisition modes of the perfusion time and the perfusion speed are as follows: selecting perfusion time and perfusion speed which can obtain optimal perfusion effect as perfusion operation modes of any small animal according to the result of multiple perfusion experiments of the small animal subdivided into the same subclass and data of relevant perfusion time and perfusion speed, wherein the experiment control unit displays a control interface on a touch screen, the control interface comprises a first interface and a second interface, the first interface is provided with type selection, strain selection, age-per-day input, weight input, a cleaning button and a perfusion button, the perfusion button is touched to enter the second interface, the second interface is provided with a channel selection button, and a start button, a pause button and a stop button are arranged above the channel selection button; the channel selection button is used for selecting perfusion operation of animal hearts in different heart fixing containers, and the controller 13 is used for controlling different liquid pumps B8 and electromagnetic valves matched with the liquid pump B8 to perfuse.
In a further embodiment, as shown in fig. 5, the heart fixing container 11 is a shell-like structure, the shell-like structure includes a housing cover a11-1 and a housing cover B11-2 hinged to each other, an inner cavity 11-3 matched with the placing shape of the animal heart is arranged in the housing cover a11-1 and the housing cover B11-2, the edges of the housing cover a11-1 and the housing cover B11-2 are respectively provided with a snap fastener a11-8 matched with each other and are connected by the snap fastener a11-8, and the edges of the housing cover a11-1 and the housing cover B11-2 are further provided with an overflow port 11-6; the puncture needle of the electromagnetic blood flow sensor 11-4 is positioned in the inner mold cavity, the puncture needle, the perfusion needle 11-5 and the inner mold cavity 11-3 are matched with the animal heart placed in the inner mold cavity, when the animal heart is placed in the inner mold cavity 11-3 and the heart fixing container 11 is closed, the puncture needle of the electromagnetic blood flow sensor 11-4 punctures the pericardium, and the perfusion needle 11-5 punctures the left ventricle; the heart fixing container 11 is provided with a plurality of inner mold cavities 11-3 with different shapes and sizes so as to adapt to the fixing requirements of different animal hearts.
In a further embodiment, as shown in fig. 4, the animal fixer is arranged in the middle of the dissecting table 20 and comprises a groove 20-1 matched with the animal body, and a chest clamp 20-2, one end of the chest clamp 20-2 is hinged with the top of one side of the groove 20-1, the other end is connected with the top of the other side of the groove 20-1 through a buckle B20-4 in a buckling mode, and the middle of the chest clamp 20-2 is provided with an operation opening 20-6.
In a further embodiment, as shown in fig. 4, a drainage groove 20-3 is further provided on the table surface of the dissecting table 20 where the periphery of the groove 20-1 is located, the drainage groove 20-3 surrounds the groove 20-1, the bottom of the drainage groove 20-3 is gradually inclined downwards towards one end, a drainage tube 20-5 is provided at the lowest part of the groove bottom, and the drainage tube 20-5 is connected with a waste liquid collecting container 23 through a connecting tube 21.
In a further embodiment, as shown in fig. 4, a transparent isolation cover 17 is disposed on the top of the perfusion chamber, an illuminating lamp 19 is disposed on the lower surface of the isolation cover 17, vent holes 18 are respectively disposed on the side walls of the two sides of the perfusion chamber, an exhaust port is further disposed on the outer wall of one end of the perfusion chamber, an exhaust fan 26 is installed at the exhaust port, one end of the exhaust port located outside the perfusion chamber is connected to a formaldehyde purifier 25 through a vent pipe 24, and the purified gas is exhausted into the atmosphere.
In a further embodiment, as shown in fig. 4, the connection tube 21 is connected to a waste liquid collection container 23 through a waste liquid delivery hose 22, and the waste liquid collection container 23 is disposed outside the perfusion chamber; the bottom of the groove 20-1 is provided with a leakage hole, and the leakage hole is connected with the connecting pipe 21 through a connecting pipe.
In a further embodiment, the controller 13 is a control chip or a control circuit board or a PLC controller.
The working principle of the invention is as follows: in a perfusion experiment, an experimental animal is anesthetized and placed in a groove 20-1 on an dissecting table 20, a chest clamp 20-2 is buckled, the heart of the animal is dissected and exposed at an operation opening 20-6, the heart of the animal is placed in a heart fixing container 11, in the process of buckling the heart fixing container, a puncture needle of an electromagnetic blood flow sensor 11-4 punctures the pericardium and enables blood to flow out, a perfusion needle 11-5 punctures the left ventricle and introduces perfusion liquid, because the experimental animal perfusion device subdivides the experimental animal according to different types, strains and weights, and further sets corresponding inner mold cavities 11-3 according to different experimental animals, the inner mold cavity 11-3, the puncture needle of the electromagnetic blood flow sensor 11-4, the perfusion needle 11-5 and the heart of the animal are matched with each other, when the heart is placed in the inner mold cavity 11-3 and the heart fixing container 11 is closed, the pericardium can be accurately punctured by the puncture needle of the electromagnetic blood flow sensor 11-4, the left ventricle can be accurately punctured by the perfusion needle 11-5, when the left ventricle is closed, the heart is tightly wrapped, and perfusion liquid and blood flow out through the overflow port 11-6.
Further, the perfusion liquid and the blood flowing out through the overflow port 11-6 flow into the drainage tube 20-5 through the drainage groove 20-3 and are finally discharged into the waste liquid collecting container 23.
Furthermore, when the intelligent perfusion device needs to be cleaned, a cleaning button is selected on the first interface, at this time, the controller 13 starts the liquid pumping pump a7 and the liquid pumping pump B8, the liquid pumping pump a7 guides deionized water in the washing bottle 4 into the buffer bottle 5 and the fixing bottle 6, and conveys the liquid to different heart fixing containers 11 through the liquid pumping pump B8, and the liquid in the heart fixing containers 11 is discharged through the overflow ports 11-6, so that the cleaning work of the intelligent perfusion device is quickly completed.
Further, when the perfusion operation is performed according to the type, strain, age in day and weight of the experimental animal, the selection is performed through the first interface displayed on the touch screen 2, the perfusion button is selected and then continuously selected, the experimental animal enters the second interface, the channel button capable of being operated by perfusion is selected according to the subdivided subclasses of the animal, after the channel button is selected, the heart of the animal is fixed in the heart fixing container 11 corresponding to the channel, and the perfusion operation is performed by pressing the channel button, when the perfusion operation is performed, the controller automatically operates according to the preset optimal perfusion speed and perfusion time of the subclass animal, specifically, the pumping time of the pumping pump B8 is perfusion time, whether the blood is flushed completely is detected through the electromagnetic blood flow sensor 11-4, the signal of flushing completely is transmitted to the controller 13, the controller 13 sends a signal of pumping the fixed liquid to the pumping pump B8 at this time, meanwhile, the controller 13 controls the solenoid valve in the liquid outlet 12 to realize liquid pumping selection.
Furthermore, the liquid pump B8 of the present invention can precisely control the input, flow rate, volume and time of liquid under the control of the controller 13, so that the perfusion efficiency is higher, and waste caused by insufficient or excessive input of liquid is avoided.
Furthermore, the buffer bottle 5 is used for containing buffer solution such as PBS, normal saline, sodium citrate solution and the like, the fixing bottle 6 is used for containing paraformaldehyde, and the washing bottle 4 is used for containing deionized water for washing paraformaldehyde, PBS and the like, so that the device can be effectively protected and prevented from being corroded.
Further, the outer surface of the side wall of the invention is also provided with a sound device, when the perfusion is finished, the controller 13 sends a signal to the sound device to remind an experimental operator of the completion of the perfusion.
Furthermore, the invention can arrange different dissecting tables 20 according to experimental animals of different types, strains, ages in days and weights, and the grooves 20-1 on the dissecting tables 20 are matched with the body types of the animals, thereby ensuring that the bodies of the animals are effectively fixed.
Further, the dissecting table 20 is arranged in the perfusion chamber, the dissecting table 20 can be used under the condition that the animal is completely anesthetized, and can also be used in cooperation with a small animal respirator, and only a respirator is required to be added.
Further, the perfusion chamber is internally provided with an exhaust fan 26, air containing formaldehyde can be discharged into the formaldehyde purifier 25 through the vent pipe 24, and the purified air is discharged into the atmosphere, so that the environmental protection is ensured, and the harm of formaldehyde gas to experimenters can be prevented.
Claims (9)
1. An intelligent rodent heart perfusion device characterized by: the intelligent perfusion device comprises a perfusion chamber, an dissecting table and an intelligent perfusion device, wherein the intelligent perfusion device comprises a shell, a liquid storage bin, a transfer bin and a control bin are arranged in the shell, an animal fixer for fixing experimental animals is arranged on the dissecting table, a liquid pump B is arranged in the transfer bin, a liquid input end of the liquid pump B is communicated with liquid in the liquid storage bin, a liquid output end of the liquid pump B is connected with a heart fixing container through a liquid conveying hose, the end part of the liquid conveying hose is connected with a perfusion needle, a sharp prick end of the perfusion needle penetrates through the outer wall of the heart fixing container and extends into the heart fixing container, an electromagnetic blood flow sensor is further arranged in the heart fixing container, a controller is arranged in the control bin, the electromagnetic blood flow sensor is connected with the controller through a signal line, and the controller is connected with the liquid pump B through an electric wire, the controller is also electrically connected with a power supply, the intelligent perfusion device is matched with an anatomical table for use, and the anatomical table is arranged in the perfusion chamber;
the heart fixing container is of a shell-like structure, the shell-like structure comprises a shell cover A and a shell cover B which are hinged with each other, inner cavities matched with the placing shapes of animal hearts are arranged in the shell cover A and the shell cover B, buckling pieces A which are matched with each other are respectively arranged at the edges of the shell cover A and the shell cover B and are connected in a buckling mode through the buckling pieces A, and overflow ports are also arranged at the edges of the shell cover A and the shell cover B; the puncture needle of the electromagnetic blood flow sensor is positioned in the inner mold cavity, the puncture needle, the perfusion needle and the inner mold cavity are matched with the animal heart placed in the inner mold cavity, when the animal heart is placed in the inner mold cavity and the heart fixing container is closed, the puncture needle of the electromagnetic blood flow sensor punctures the pericardium, and the perfusion needle punctures the left ventricle; the heart fixing containers are multiple, and inner mold cavities with different shapes and sizes are arranged to meet the fixing requirements of different animal hearts.
2. The intelligent rodent heart perfusion device of claim 1, wherein: a washing bottle for containing cleaning liquid, a buffer bottle for containing buffer liquid and a fixing bottle for containing fixing liquid are arranged in the liquid storage bin; the transfer bin is internally provided with 1 liquid pump A and a plurality of liquid pumps B, the bottoms of the flushing bottle, the buffer bottle and the fixed bottle are all provided with liquid outlets, the washing bottle is provided with 1 liquid outlet and is connected with the liquid input end of the liquid pump A through a liquid conveying pipe, the liquid output end of the liquid pump A is connected with a three-way joint, one end of the three-way joint is connected with the liquid pump A, the other two ends are respectively communicated with the buffer bottle and the fixed bottle through liquid conveying pipes which run through the top ends of the buffer bottle and the fixed bottle, the bottom of buffer bottle, fixed bottle be equipped with a plurality of liquid outlets, the liquid outlet of buffer bottle or fixed bottle be connected with the liquid input of drawing liquid pump B of difference through the liquid conveying pipe respectively, arbitrary the liquid outlet in all be equipped with the solenoid valve, the controller respectively with solenoid valve, drawing liquid pump A electric connection.
3. The intelligent rodent heart perfusion device of claim 2, wherein: the control cabin still be equipped with the touch-sensitive screen, the display opening of touch-sensitive screen in the preceding terminal surface of the casing at control cabin place still be equipped with switch button before on the terminal surface, the controller pass through the electric wire with the touch-sensitive screen and be connected with the signal line.
4. The intelligent rodent heart perfusion device of claim 3, wherein: the touch screen is also internally provided with an experiment control unit, the experiment control unit subdivides the operation modes according to the types, strains, ages in days and weights of experimental animals to classify the operation modes, the operation modes are set to be specific perfusion time and perfusion speed, the electromagnetic valve, the liquid pump A and the liquid pump B are controlled by the controller to be specifically implemented, and the acquisition modes of the perfusion time and the perfusion speed are as follows: selecting perfusion time and perfusion speed which can obtain optimal perfusion effect as perfusion operation modes of any small animal according to the result of multiple perfusion experiments of the small animal subdivided into the same subclass and data of relevant perfusion time and perfusion speed, wherein the experiment control unit displays a control interface on a touch screen, the control interface comprises a first interface and a second interface, the first interface is provided with type selection, strain selection, age-per-day input, weight input, a cleaning button and a perfusion button, the perfusion button is touched to enter the second interface, the second interface is provided with a channel selection button, and a start button, a pause button and a stop button are arranged above the channel selection button; the channel selection button is used for selecting perfusion operation of animal hearts in different heart fixing containers, and the controller controls different liquid pumps B and electromagnetic valves matched with the liquid pumps B to perfuse.
5. The intelligent rodent heart perfusion device of any one of claims 1-4, wherein: the animal fixer is arranged in the middle of the dissecting table and comprises a groove matched with the animal body and a chest clamp, one end of the chest clamp is hinged with the top of the groove wall on one side of the groove, the other end of the chest clamp is connected with the top of the groove wall on the other side of the groove through a fastener B in a buckling mode, and an operation opening is formed in the middle of the chest clamp.
6. The intelligent rodent heart perfusion device of claim 5, wherein: the table-board of the dissection table at the periphery of the groove is also provided with a drainage groove, the drainage groove surrounds the groove, the groove bottom of the drainage groove gradually inclines downwards to one end and extends, and a drainage tube is arranged at the lowest part of the groove bottom and is connected with a waste liquid collecting container through a connecting pipe.
7. The intelligent rodent heart perfusion device of claim 6, wherein: the top of perfusion chamber be equipped with transparent cage, be equipped with the light at the lower surface of cage, be equipped with the blow vent on the lateral wall of the both sides of perfusion chamber respectively, the outer wall of the one end of perfusion chamber on still be equipped with the gas vent, install the air discharge fan in gas vent department, the gas vent be located the outdoor one end of perfusion chamber and be connected with the formaldehyde purification ware through the breather pipe, the atmosphere is discharged to the gas after the purification.
8. The intelligent rodent heart perfusion device of claim 6, wherein: the connecting pipe is connected with a waste liquid collecting container through a waste liquid conveying hose, and the waste liquid collecting container is arranged outside the perfusion chamber; and a liquid leakage hole is formed at the bottom of the groove and is connected with the connecting pipe through a connecting pipe.
9. The intelligent rodent heart perfusion device of any one of claims 1-4 and 6-8, wherein: the controller is a control chip or a control circuit board or a PLC controller.
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| CN102614030B (en) * | 2012-04-18 | 2014-12-17 | 新疆维吾尔自治区人民医院 | Device for fixing experimental animal during perfusion and application thereof |
| CN202637184U (en) * | 2012-07-26 | 2013-01-02 | 黑河学院 | Device for vein haemospasia and fixation of rat |
| CN102813559B (en) * | 2012-08-27 | 2014-11-05 | 山东大学 | Full-automatic hemoperfusion device |
| CN104568542A (en) * | 2014-12-30 | 2015-04-29 | 燕山大学 | Method for fixing fish brain tissue specimen through systemic heart perfusion |
| CN104644284B (en) * | 2015-02-26 | 2017-01-18 | 中国人民解放军第二军医大学 | Small animal perfusion sampling fixing device and small animal perfusion sampling fixing method |
| CN204890252U (en) * | 2015-07-01 | 2015-12-23 | 上海钰森生物技术有限公司 | Small -size experimental animals is system of filling in batches |
| DE102016225167B4 (en) * | 2016-12-15 | 2018-10-11 | Otto-Von-Guericke-Universität Magdeburg | Ex vivo pulmonary circulation and ventilation device and method for training surgical and minimally invasive surgical procedures |
| CN206443022U (en) * | 2017-01-19 | 2017-08-29 | 昆明医科大学 | A kind of device for casting of experimental animal |
| CN208160675U (en) * | 2017-08-10 | 2018-11-30 | 武汉瑞福宁科技有限公司 | The perfusion device of self-retaining animal hearts |
| CN208426261U (en) * | 2017-12-11 | 2019-01-25 | 齐齐哈尔医学院 | A kind of automatic filling system fixed suitable for rats and mice intracerebral |
| CN208808723U (en) * | 2018-04-03 | 2019-05-03 | 华中科技大学苏州脑空间信息研究院 | Toy perfusion autopsy table |
| CN208218830U (en) * | 2018-04-03 | 2018-12-11 | 华中科技大学苏州脑空间信息研究院 | A kind of automatic perfusion device |
| CN208799368U (en) * | 2018-04-16 | 2019-04-30 | 新乡医学院 | Synchronize mouse heart perfusion combination unit |
| CN210131647U (en) * | 2019-04-16 | 2020-03-10 | 天津市康婷生物工程集团有限公司 | Be applicable to special dissection table of brain is got in rat heart perfusion |
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