CN104641896A - Cottage propagation method of stem of rumex hastatus - Google Patents
Cottage propagation method of stem of rumex hastatus Download PDFInfo
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- CN104641896A CN104641896A CN201510043487.6A CN201510043487A CN104641896A CN 104641896 A CN104641896 A CN 104641896A CN 201510043487 A CN201510043487 A CN 201510043487A CN 104641896 A CN104641896 A CN 104641896A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
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Abstract
The invention discloses a cottage propagation method of a stem of rumex hastatus. The method comprises the following steps of: (1) preparing cutting slips; (2) preparing cottage matrix; (3) preparing rooting agent; (4) processing the cutting slips and cottage; (5) managing and transplanting after inserting. According to the cottage method of the stem of the rumex hastatus, cottage survival rate is more than 92%, a large amount of seedlings can be obtained within a short time, and requirements on large amount of seedlings for ecological rehabilitation project of five mining areas are met. The method is simple and convenient to operate, low in production cost, simple in technical requirements and easy to popularize and use; and the method has important application and popularization value on cottage propagation and production of the stem of the rumex hastatus.
Description
Technical field
The invention belongs to plant cultivation method technical field, relate to a Plants asexual reproduction method, particularly a kind of cuttage breeding method of Rheum hastatus D. Don stem section.
Background technology
Rheum hastatus D. Don (Rumex hastatus D.Don), another name: large garden sorrel, ephedra sinica, native Chinese ephedra, Rumex madaio, Yunnan, river garden sorrel.For Liao Ke (Polygonaceae) Rumex (Rumex) perennial plant.Stem multi-branched, old branch is wooden, dark violet brown; Annual branch herbaceous stem, green.Leaf alternate or cluster, halberd shape.Be born in the places such as the dry-hot valley of height above sea level 300 ~ 2700m, shrubbery, Lin Zhong, small stream limit, dry roadside, stoneledge, be common in hillside abandoned land; Its happiness environment on the sunny side, drought-resistant, barren-resistant.Crack of stone all energy normal growths of the hillside fields that, soil layer barren at soil drought is very thin or exposed bedrock, the Pioneer plant community of Chang Zuowei In Limestone Area occurs.Its well developed root system, elongated and in depth under, can through the loose weathered layer of slope body shallow-layer, anchor on the more stable rock-soil layer in depths, there is good anchoring to Soil Slope and prevent corrosion function, can administer for the revegetation in " five exploiting fields " such as " quarry, adopt sand, dig up mine, fetch earth, brick and tile kiln ground " in a large number, be the good plant of restoration of the ecosystem.By literature search, the domestic cuttage breeding method that there is no Rheum hastatus D. Don stem section.
Summary of the invention
For solving above-mentioned prior art Problems existing, the object of the present invention is to provide a kind of cuttage breeding method of Rheum hastatus D. Don stem section, the method can obtain a large amount of Rheum hastatus D. Don seedling within a short period of time, is beneficial to the Ecological Restoration Project of mine and side slope.The object of the present invention is to provide a kind of easy to operation, with low cost, reproduction rate is high.
For achieving the above object, technical scheme of the present invention is:
A cuttage breeding method for Rheum hastatus D. Don stem section, comprises the steps:
(1) cuttings is prepared: select the healthy life in 2 years without damage by disease and insect or triennial lignification branch as Rheum hastatus D. Don cuttings, clip length is 6 ~ 8cm, and the upper and lower clip of cuttings is truncate, and clip is all apart from bud 0.5 ~ 1cm, and all blades are all cut;
(2) cutting medium is equipped with: perlite, laterite, humus soil are hybridly prepared into cutting medium according to the volume ratio of 1: 1: 1 ~ 1: 2: 1, with 50% carbendazol wettable powder dilution, 500 ~ 750 times of medicine liquid spray sterilization cutting mediums;
(3) root-growing agent is prepared: the kind of root-growing agent adopts the built agent of indolebutyric acid and naa, mass ratio is: 1: 4, concentration is 30 ~ 50mg/L, indolebutyric acid 6.0 ~ 10.0mg and chemical pure methyl α-naphthyl acetate 10.0 ~ 40.0mg is dissolved in the ethanol of 95% of 10 ~ 20ml respectively, after dissolving completely, mixing and water adding, to 1000ml, makes composite root-growing agent after shaking up again;
(4) cuttings process and cuttage: by the base portion of cuttings respectively clear water and 0.1% disinfecting solution of potassium permanganate process 2 ~ 3min after, insert in the root-growing agent that step (3) prepares and soak 10min, puncture on the cutting medium be equipped with in step (2) with bamboo let, finally cuttings is inserted in the hole, compacting;
(5) management and transplanting after inserting: the cuttings that step (4) is plugged is placed in Small plastic shed, bow member can with the bamboo chip of wide 2 ~ 3cm, length is determined, general sagitta 60 ~ 80cm, the white film that plastic film 0.08mm is thick according to sagitta and seedbed height; Cover the sunshade net that one deck light transmittance is 50% again, reduce the rising dehydration of cuttings, after 10d, remove sunshade net; Every 3 ~ 5d checks the subenvironment of cuttings in a shed, and humidity film there to be the globule, be advisable by not landing.If cross in shed wet or temperature is too high, will take off film at two and ventilate, overdrying waters in time, but crosses wet in order to avoid rotten seedling.After 20 ~ 25d, cuttings base portion starts to form callus; Callus director from cuttings base portion after 30 ~ 35d goes out fibrous root; Remove Small plastic shed after 50 ~ 60d, start to carry out hardening, reduce humidity, avoid sprouting excessive growth, promote root growth; Can transplant after 80 ~ 90d.
Further, in described step (3), the root-growing agent of preparation, according to the ratio of 1: 800, adds 50% carbendazol wettable powder simultaneously.
Further, in described step (4), cuttings depth requirements: cuttings 1/2 to be inserted in cutting medium and watered permeable.
Relative to prior art, beneficial effect of the present invention is:
There is no the relevant report of the cuttage breeding method of Rheum hastatus D. Don stem section at present, initiative of the present invention first time comprehensive system provide the cuttage breeding method of Rheum hastatus D. Don stem section, fill up the blank of Rheum hastatus D. Don Technique on Cuttage Propagation method aspect.The cuttage breeding method of Rheum hastatus D. Don stem section of the present invention, its cuttage survival rate can reach more than 92%, this method is easy to operation, have that cost is low, technical requirement is simple, the feature such as easily to apply, on Rheum hastatus D. Don seedling plant modification is produced, there is important application and promotional value.
Embodiment
Below in conjunction with embodiment, technical solution of the present invention is described in further detail:
Embodiment 1
A cuttage breeding method for Rheum hastatus D. Don stem section, comprises the following steps:
(1) cuttings is prepared: select the healthy life in 2 years without damage by disease and insect of Rheum hastatus D. Don or triennial lignification branch, clip length is 6 ~ 8cm, and the upper and lower clip of cutting is truncate, and clip is all apart from bud 0.5 ~ 1cm, and all blades are all cut.
(2) cutting medium is equipped with: perlite, laterite, humus soil are hybridly prepared into cutting medium according to the volume ratio of 1: 1: 1, with 50% carbendazol wettable powder dilution, 500 ~ 750 times of medicine liquid spray sterilization cutting mediums.
(3) root-growing agent is prepared: the kind of root-growing agent adopts the built agent of indolebutyric acid and naa, and mass ratio is: 1: 4, and concentration is 30mg/L.Concrete preparation is dissolved in the ethanol of 95% of 10 ~ 20ml respectively by indolebutyric acid 6.0mg and chemical pure methyl α-naphthyl acetate 24.0mg, and after dissolving completely, mixing and water adding, to 1000ml, makes composite root-growing agent after shaking up again.For disease prevention, according to the ratio of 1: 800, add 50% carbendazol wettable powder simultaneously.
(4) cuttings process and cuttage: by the base portion of cuttings respectively clear water and 0.1% disinfecting solution of potassium permanganate process 2 ~ 3min after, insert in the root-growing agent that step (3) prepares and soak 10min, puncture on the cutting medium be equipped with in step (2) with bamboo let, finally insert in the hole 1/2 of cuttings, compacting is also watered permeable.
(5) management and transplanting after inserting: the cuttings that step (4) is plugged is placed in Small plastic shed, bow member can with the bamboo chip of wide 2 ~ 3cm, length is determined, general sagitta 60 ~ 80cm, the white film that plastic film 0.08mm is thick according to sagitta and seedbed height; Cover the sunshade net that one deck light transmittance is 50% again, after 10d, remove sunshade net; Every 3 ~ 5d checks the subenvironment of cuttings in a shed, and humidity film there to be the globule, be advisable by not landing.If cross in shed wet or temperature is too high, will take off film at two and ventilate, overdrying waters in time, but crosses wet in order to avoid rotten seedling.After 25d, cuttings base portion starts to form callus; Callus director from cuttings base portion after 35d goes out fibrous root; Remove Small plastic shed after 50 ~ 60d, start to carry out hardening, reduce humidity, avoid sprouting excessive growth, promote root growth; Can transplant after 80 ~ 90d.Cuttage survival rate reaches 92.6%.
Embodiment 2
A cuttage breeding method for Rheum hastatus D. Don stem section, comprises the following steps:
(1) cuttings is prepared: select the healthy life in 2 years without damage by disease and insect of Rheum hastatus D. Don or triennial lignification branch, clip length is 6 ~ 8cm, and the upper and lower clip of cutting is truncate, and clip is all apart from bud 0.5 ~ 1cm, and all blades are all cut.
(2) cutting medium is equipped with: perlite, laterite, humus soil are hybridly prepared into cutting medium according to the volume ratio of 1: 1: 1, with 50% carbendazol wettable powder dilution, 500 ~ 750 times of medicine liquid spray sterilization cutting mediums.
(3) root-growing agent is prepared: the kind of root-growing agent adopts the built agent of indolebutyric acid and naa, and mass ratio is: 1: 4, and concentration is 40mg/L.Concrete preparation is dissolved in the ethanol of 95% of 10 ~ 20ml respectively by indolebutyric acid 8.0mg and chemical pure methyl α-naphthyl acetate 32.0mg, and after dissolving completely, mixing and water adding, to 1000ml, makes composite root-growing agent after shaking up again.For disease prevention, according to the ratio of 1: 800, add 50% carbendazol wettable powder simultaneously.
(4) cuttings process and cuttage: by the base portion of cuttings respectively clear water and 0.1% disinfecting solution of potassium permanganate process 2 ~ 3min after, insert in the root-growing agent that step (3) prepares and soak 10min, puncture on the cutting medium be equipped with in step (2) with bamboo let, finally insert in the hole 1/2 of cuttings, compacting is also watered permeable.
(5) management and transplanting after inserting: the cuttings that step (4) is plugged is placed in Small plastic shed, bow member can with the bamboo chip of wide 2 ~ 3cm, length is determined, general sagitta 60 ~ 80cm, the white film that plastic film 0.08mm is thick according to sagitta and seedbed height; Cover the sunshade net that one deck light transmittance is 50% again, after 10d, remove sunshade net; Every 3 ~ 5d checks the subenvironment of cuttings in a shed, and humidity film there to be the globule, be advisable by not landing.If cross in shed wet or temperature is too high, will take off film at two and ventilate, overdrying waters in time, but crosses wet in order to avoid rotten seedling.After 20d, cuttings base portion starts to form callus; Callus director from cuttings base portion after 30d goes out fibrous root; Remove Small plastic shed after 50 ~ 60d, start to carry out hardening, reduce humidity, avoid sprouting excessive growth, promote root growth; Can transplant after 80d.Cuttage survival rate reaches 95.7%.
Embodiment 3
A cuttage breeding method for Rheum hastatus D. Don stem section, comprises the following steps:
(1) cuttings is prepared: select the healthy life in 2 years without damage by disease and insect of Rheum hastatus D. Don or triennial lignification branch, clip length is 6 ~ 8cm, and the upper and lower clip of cutting is truncate, and clip is all apart from bud 0.5 ~ 1cm, and all blades are all cut.
(2) cutting medium is equipped with: perlite, laterite, humus soil are hybridly prepared into cutting medium according to the volume ratio of 1: 1: 1, with 50% carbendazol wettable powder dilution, 500 ~ 750 times of medicine liquid spray sterilization cutting mediums.
(3) root-growing agent is prepared: the kind of root-growing agent adopts the built agent of indolebutyric acid and naa, and mass ratio is: 1: 4, and concentration is 50mg/L.Concrete preparation is dissolved in the ethanol of 95% of 10 ~ 20ml respectively by indolebutyric acid 10.0mg and chemical pure methyl α-naphthyl acetate 40.0mg, and after dissolving completely, mixing and water adding, to 1000ml, makes composite root-growing agent after shaking up again.For disease prevention, according to the ratio of 1: 800, add 50% carbendazol wettable powder simultaneously.
(4) cuttings process and cuttage: by the base portion of cuttings respectively clear water and 0.1% disinfecting solution of potassium permanganate process 2 ~ 3min after, insert in the root-growing agent that step (3) prepares and soak 10min, puncture on the cutting medium be equipped with in step (2) with bamboo let, finally insert in the hole 1/2 of cuttings, compacting is also watered permeable.
(5) management and transplanting after inserting: the cuttings that step (4) is plugged is placed in Small plastic shed, bow member can with the bamboo chip of wide 2 ~ 3cm, length is determined, general sagitta 60 ~ 80cm, the white film that plastic film 0.08mm is thick according to sagitta and seedbed height; Cover the sunshade net that one deck light transmittance is 50% again, after 10d, remove sunshade net; Every 3 ~ 5d checks the subenvironment of cuttings in a shed, and humidity film there to be the globule, be advisable by not landing.If cross in shed wet or temperature is too high, will take off film at two and ventilate, overdrying waters in time, but crosses wet in order to avoid rotten seedling.After 23d, cuttings base portion starts to form callus; Callus director from cuttings base portion after 35d goes out fibrous root; Remove Small plastic shed after 50 ~ 60d, start to carry out hardening, reduce humidity, avoid sprouting excessive growth, promote root growth; Can transplant after 80d.Cuttage survival rate reaches 93.3%.
Embodiment 4
A cuttage breeding method for Rheum hastatus D. Don stem section, comprises the following steps:
(1) cuttings is prepared: select the healthy life in 2 years without damage by disease and insect of Rheum hastatus D. Don or triennial lignification branch, clip length is 6 ~ 8cm, and the upper and lower clip of cutting is truncate, and clip is all apart from bud 0.5 ~ 1cm, and all blades are all cut.
(2) cutting medium is equipped with: perlite, laterite, humus soil are hybridly prepared into cutting medium according to the volume ratio of 1: 2: 1, with 50% carbendazol wettable powder dilution, 500 ~ 750 times of medicine liquid spray sterilization cutting mediums.
(3) root-growing agent is prepared: the kind of root-growing agent adopts the built agent of indolebutyric acid and naa, and mass ratio is: 1: 4, and concentration is 40mg/L.Concrete preparation is dissolved in the ethanol of 95% of 10 ~ 20ml respectively by indolebutyric acid 8.0mg and chemical pure methyl α-naphthyl acetate 32.0mg, and after dissolving completely, mixing and water adding, to 1000ml, makes composite root-growing agent after shaking up again.For disease prevention, according to the ratio of 1: 800, add 50% carbendazol wettable powder simultaneously.
(4) cuttings process and cuttage: by the base portion of cuttings respectively clear water and 0.1% disinfecting solution of potassium permanganate process 2 ~ 3min after, insert in the root-growing agent that step (3) prepares and soak 10min, puncture on the cutting medium be equipped with in step (2) with bamboo let, finally insert in the hole 1/2 of cuttings, compacting is also watered permeable.
(5) management and transplanting after inserting: the cuttings that step (4) is plugged is placed in Small plastic shed, bow member can with the bamboo chip of wide 2 ~ 3cm, length is determined, general sagitta 60 ~ 80cm, the white film that plastic film 0.08mm is thick according to sagitta and seedbed height; Cover the sunshade net that one deck light transmittance is 50% again, after 10d, remove sunshade net; Every 3 ~ 5d checks the subenvironment of cuttings in a shed, and humidity film there to be the globule, be advisable by not landing.If cross in shed wet or temperature is too high, will take off film at two and ventilate, overdrying waters in time, but crosses wet in order to avoid rotten seedling.After 20d, cuttings base portion starts to form callus; Callus director from cuttings base portion after 30d goes out fibrous root; Remove Small plastic shed after 50 ~ 60d, start to carry out hardening, reduce humidity, avoid sprouting excessive growth, promote root growth; Can transplant after 90d.Cuttage survival rate reaches 93.7%.
Wherein with embodiment 2 for preferred embodiment.
The above, be only the specific embodiment of the present invention, but protection scope of the present invention is not limited thereto, and any change of expecting without creative work or replacement, all should be encompassed within protection scope of the present invention.Therefore, the protection domain that protection scope of the present invention should limit with claims is as the criterion.
Claims (3)
1. a cuttage breeding method for Rheum hastatus D. Don stem section, is characterized in that, comprises the steps:
(1) cuttings is prepared: select the healthy life in 2 years without damage by disease and insect or triennial lignification branch as Rheum hastatus D. Don cuttings, clip length is 6 ~ 8cm, and the upper and lower clip of cuttings is truncate, and clip is all apart from bud 0.5 ~ 1cm, and all blades are all cut;
(2) cutting medium is equipped with: perlite, laterite, humus soil are hybridly prepared into cutting medium according to the volume ratio of 1: 1: 1 ~ 1: 2: 1, with 50% carbendazol wettable powder dilution, 500 ~ 750 times of medicine liquid spray sterilization cutting mediums;
(3) root-growing agent is prepared: the kind of root-growing agent adopts the built agent of indolebutyric acid and naa, mass ratio is: 1: 4, concentration is 30 ~ 50mg/L, indolebutyric acid 6.0 ~ 10.0mg and chemical pure methyl α-naphthyl acetate 10.0 ~ 40.0mg is dissolved in the ethanol of 95% of 10 ~ 20ml respectively, after dissolving completely, mixing and water adding, to 1000ml, makes composite root-growing agent after shaking up again;
(4) cuttings process and cuttage: by the base portion of cuttings respectively clear water and 0.1% disinfecting solution of potassium permanganate process 2 ~ 3min after, insert in the root-growing agent that step (3) prepares and soak 10min, puncture on the cutting medium be equipped with in step (2) with bamboo let, finally cuttings is inserted in the hole, compacting;
(5) management and transplanting after inserting: the cuttings that step (4) is plugged is placed in Small plastic shed, bow member can with the bamboo chip of wide 2 ~ 3cm, length is determined, general sagitta 60 ~ 80cm, the white film that plastic film 0.08mm is thick according to sagitta and seedbed height; Cover the sunshade net that one deck light transmittance is 50% again, reduce the rising dehydration of cuttings, after 10d, remove sunshade net; Every 3 ~ 5d checks the subenvironment of cuttings in a shed, and humidity film there to be the globule, be advisable by not landing, if the interior mistake of shed is wet or temperature is too high, film to be taken off ventilate at two, overdrying waters in time, but does not cross wet in order to avoid rotten seedling, and after 20 ~ 25d, cuttings base portion starts to form callus; Callus director from cuttings base portion after 30 ~ 35d goes out fibrous root; Remove Small plastic shed after 50 ~ 60d, start to carry out hardening, reduce humidity, avoid sprouting excessive growth, promote root growth; Can transplant after 80 ~ 90d.
2. method according to claim 1, is characterized in that, in described step (3), the root-growing agent of preparation, according to the ratio of 1: 800, adds 50% carbendazol wettable powder simultaneously.
3. method according to claim 1, is characterized in that, in described step (4), and cuttings depth requirements: cuttings 1/2 to be inserted in cutting medium and watered permeable.
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CN108966881A (en) * | 2018-08-24 | 2018-12-11 | 昆明市濒危动植物收容拯救中心 | Endangered species Poncirus polyandra cuttage breeding method |
CN108966881B (en) * | 2018-08-24 | 2020-09-18 | 昆明市濒危动植物收容拯救中心 | Cutting propagation method for citrus aurantium in endangered species |
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