CN104622998A - Applications of fructus sorbus sibirica extract in preparation of lipid-lowering medicament - Google Patents

Abstract

The invention relates to applications of fructus sorbus sibirica extract in preparation of a lipid-lowering medicament. The fructus sorbus sibirica extract can be used alone or combined with other lipid-lowering medicaments, to prepare medicaments or healthy products of any dosage form suitable for oral administration. The fructus sorbus sibirica extract has strong lipid-lowering capacity.

Description

The application of Sorbus sibirica fruit extract in preparation treatment fat-reducing medicament

Technical field

The invention belongs to the purposes field of medicine, relate to Sorbus sibirica fruit extract particularly and preparing the application in the medicine or health product with effect for reducing fat.

Background technology

The content of one or more lipids that hyperlipidemia refers in blood exceedes disease during normal high limit, also can be described as hyperlipoproteinemia, epidemiologic data shows, be 18.6% in the prevalence of Chinese adult dyslipidemia, that is, the number of patients with dyslipidemia reaches 100,000,000 6 thousand ten thousand, also it was reported that the whole world every year nearly 1,200 ten thousand people die from cardiovascular diseases and apoplexy, hyperlipemia is the pathological changes basis of AS, main manifestations is plasma TC and (or) TG, and (or) HDL-C level is too low, therefore prevention and therapy hyperlipemia receives the extensive attention of medical circle.

And existing Therapeutic Method mostly adopts the medicine of chemosynthesis, its cost is large, and toxicity is large, and side effect is strong, and such as statins has similar myalgia, affects coenzyme Q10 synthesis, affects the side effect such as health of heart further.Therefore, find from natural plants and there is material that is bioactive, antilipemic effect be applied in medicine, food and health product and have great importance.

Sorbus sibirica is Rosaceae (Rosaceae) Fructus Sorbi Pohuashanensis, belongs to dungarunga, is only distributed in upstream, Burqin river in China, under liking being grown on PiceameyeriRehd. Et Wils. and fir mixed forest, its tree-like grace, figure hold directly, and leaf is large-scale winglike compound leaf, is rich in ornamental value.Spring, snow-white flower completely set fragrance, and the scarlet wish of mellow fruit in autumn is dripped.It is edible that fruit is rich in multivitamin, has ascorbic acid (200mg/100g), and carotene (18mg/100g), VP, Sorbitol, organic acid, VC etc., its fresh fruit Polyphenols content reaches 25% ~ 35%.

Summary of the invention

The present invention is for the purpose of the exploitation for seeking new and effective blood fat reducing medicine or health product, proposes the application of Sorbus sibirica fruit extract in the medicine preparing effect for reducing blood fat or health product.

The present invention adopts Sorbus sibirica fruit extract as raw material, alone or with other, there is the drug regimen of effect for reducing blood fat, the preparation method of preparation routinely, be prepared into the medicine with effect for reducing blood fat or the health product of applicable oral any dosage form, as oral liquid, tablet, capsule, granule etc.; Or other injection dosage form.

What the present invention adopted is Sorbus sibirica fruit alcohol extraction extract, drawn by hyperlipemia model mice experiment in vivo, Sorbus sibirica fruit extract can reduce cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), HDL-C (HDL-C) content in hyperlipemia in mice serum, and liver is heavy.Therefore, Sorbus sibirica fruit extract has effect of blood fat reducing regulating liver-QI fat, is a good selection of the efficient natural fat-reducing medicament of development of new or health product.

Detailed description of the invention

The application of embodiment 1 Sorbus sibirica fruit extract in preparation treatment fat-reducing medicament

(1) preparation method

The preparation of Sorbus sibirica fruit extract: take Sorbus sibirica fruit 100g, ethanol with 60% soaks 1h at 60-70 DEG C, put into the ultrasonic 30min of ultrasonic cleaner, continuous 2 times, use Rotary Evaporators concentrated extracting solution again, obtain Sorbus sibirica fruit extract dry powder through vacuum lyophilization.

(2) test of pesticide effectiveness

Animal feeding and process: within 1 week, to adapt to this laboratory condition, sub-cage rearing, indoor Aseptic sterilisation, changes bedding and padding in every two days to experimental animal feeding.Bright-the 12h of Control release room illumination 12h is dark, and indoor temperature is 20 ± 1 DEG C, and humidity is 55 ± 10%.

Laboratory animal divide into groups: male mice 60, body weight 30 ± 2g, be divided at random 5 groups as follows, often group 10.

(1) Normal group (Normal group, N);

(2) hyperlipidemia model group (High-lipid diet model group, H);

(3) Fructus Sorbi Pohuashanensis fruit extract low dose group (polyphenol lower dose group, PL) (0.3mg/d);

(4) dosage group (polyphenol middle dose group, PM) (1.5mg/d) in Fructus Sorbi Pohuashanensis fruit extract;

(5) Fructus Sorbi Pohuashanensis fruit extract high dose group (polyphenol higher dose group, PH) (3mg/d);

After conforming one week, except normal control, other is respectively organized mice and all changes and feed high lipid food (10% Adeps Sus domestica, 1% cholesterol, 15% sucrose) and bring out hyperlipemia, often organizes ad lib and drinking-water, Fructus Sorbi Pohuashanensis fruit extract doses group gastric infusion.In test, a body weight was claimed to change single administration dosage every 7 days.When experiment the 21st day, fasting 12h (can't help water), extract eyeball and get blood, dislocation is put to death, get liver, detect TC, TG, LDL-C, HDL-C level content in blood and liver homogenate, according to TC, TG, LDL-C, HDL-C index in serum in Shenyang Ding Guo Bioisystech Co., Ltd test kit description method mensuration Mice Body.Result is as table 1.

The impact that table 1 Sorbus sibirica fruit polyphenol weighs lipid of mice level and liver

As can be seen from Table 1, compared with normal group mice, TC, TG, LDL-C level content in model group mice serum and liver weight average are significantly higher than Normal group (P<0.05), HDL-C is significantly lower than matched group (P<0.05), show to feed high lipid food by this test method to mice, can successfully set up hyperlipidemia mouse model.

Compare with hyperlipidemia model group, the basic, normal, high dosage group of Sorbus sibirica fruit extract, can significantly reduce TC, TG, LDL-C, HDL-C level content, and liver is heavy and body weight.Fructus Sorbi Pohuashanensis fruit extract using dosage is not high simultaneously, and Be very effective is described.

Result shows that Sorbus sibirica fruit extract has the effect obviously reducing TC, TG, LDL-C, HDL-C content, reduces liver and weighs and body weight, illustrate that it has effect of blood fat reducing regulating liver-QI fat.Conclusion: the ability of Sorbus sibirica fruit extract blood fat reducing is strong is a good selection of the efficient natural fat-reducing medicament of development of new or health product.

Claims (4)

1. Sorbus sibirica fruit extract is alone or preparing the application for the treatment of in fat-reducing medicament with other drug regimens with effect for reducing fat.

2. apply as claimed in claim 1, it is characterized in that: described Sorbus sibirica fruit extract is alcohol extraction compound.

3. apply as claimed in claim 1 or 2, it is characterized in that: the preparation of described Sorbus sibirica fruit extract: take Sorbus sibirica fruit 100g, ethanol with 60% soaks 1h at 60-70 DEG C, put into the ultrasonic 30min of ultrasonic cleaner, continuous 2 times, use Rotary Evaporators concentrated extracting solution again, obtain Sorbus sibirica fruit extract dry powder through vacuum lyophilization.

4. apply as claimed in claim 1 or 2, it is characterized in that: the dosage of described Sorbus sibirica fruit extract is 0.3-3mg/d.