CN104622722B - Anti-ultraviolet radiation sun cream - Google Patents
Anti-ultraviolet radiation sun cream Download PDFInfo
- Publication number
- CN104622722B CN104622722B CN201510064458.8A CN201510064458A CN104622722B CN 104622722 B CN104622722 B CN 104622722B CN 201510064458 A CN201510064458 A CN 201510064458A CN 104622722 B CN104622722 B CN 104622722B
- Authority
- CN
- China
- Prior art keywords
- skin
- ultraviolet
- ultraviolet radiation
- group
- cream
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Cosmetics (AREA)
Abstract
The invention discloses an anti-ultraviolet radiation sun cream, which comprises the following active components: (7-acetoxyl-2-(acetoxyl methyl)-1-((4-acetoxyl benzene) acetenyl)-9H-fluorene-9,9-di-substituted) bi(4,1-phenylene) glacial acetic acid or a derivative thereof. The antioxidant capacity of ultraviolet-radiated skin can be improved; removal of oxygen free radicals can be accelerated; amino acid metabolism of skin tissues after ultraviolet radiation is adjusted; and the content of skin collagen is increased, s that the anti-ultraviolet radiation sun cream plays a protecting role in the ultraviolet-radiated skin, and skin photoaging is delayed.
Description
Technical field
The invention belongs to technical field of skin care, in particular to a kind of sunscreen cream of ultra-violet radiation resisting.
Background technology
As ozone layer is increasingly thinning, the ultraviolet for reaching ground is on the increase.The uitraviolet intensity that sunlight is contained within is very
Height, summer direct sunlight skin, ultraviolet easy penetrating skin surface, ultraviolet irradiation can not only produce melanin, make skin
Blackening, also makes the possibility of skin ageing, after skin receives ultraviolet overexposure, can damage epidermis cell.Sunlight draws
The great majority that damage for rising are caused by the ultraviolet irradiation that wavelength is 200 to 400nm.UVC base of its medium wavelength less than 290nm
Absorbed by ozone layer on this, it is impossible to reach ground；It is heavy with skin pigment that wave-length coverage is that the UVB of 290 to 320nm can cause to burn
；Wave-length coverage be the UVA of 320 to 400nm can cause slightly to burn with it is tanned.Sun-prevention component generally used in cosmetics
Such as nano titanium oxide, Zinc Oxide, benzophenone, due to there are problems that such as skin irritation, blocking pore,
There is strict consumption to limit.The sunscreen of natural botanical source enjoys consumers because of its wide spectrum, safe sun-proof result.
CN103588616A discloses a kind of Selaginella pulvinata extract and its preparation method and application, carries for Selaginella pulvinata
Take thing to conduct in-depth research, with various column chromatography separation technologies and half preparative high-performance liquid chromatographic separation method, extract
It is separated to the compound that a class has the new framing structure of 9,9- diphenyl -1- (phenylacetylene base) -9H- fluorenes（DZJB1-13）, and drape over one's shoulders
Revealing these compounds can significantly inhibit 4 type phosphodiesterases（PDE4）Activity, can be used for prepare PDE4 inhibitor, treatment roar
Breathe heavily, chronic obstructive pneumonia, inflammation etc..At present, still cause in preventing and treating ultraviolet without after this kind of compound external of document report
Skin injury in terms of biological activity.
The content of the invention
It is an object of the invention to be studied by the biological activity to native compound monomer, there is provided a kind of uvioresistant
The sunscreen cream of beta radiation.The sunscreen cream is with (7- acetoxyl group -2- (acetoxy-methyl) -1- ((4- acetyloxy phenyls) acetylene
Base) replacements of -9H- fluorenes -9,9- two) two (4,1- phenylenes) diacetate esters（DZJB-13）Or derivatives thereof be active component, to purple
Mice Photoaging model caused by outside line has the protective action of highly significant, can be developed into the sun-proof of ultra-violet radiation resisting
Frost.
In order to realize the purpose of the present invention, inventor is studied and persistent exploration by a large amount of animal experiments, is finally obtained
Following technical scheme：
A kind of sunscreen cream of ultra-violet radiation resisting, the sunscreen cream is prepared from by active component and adjuvant, described activity
Composition includes that (7- acetoxyl group -2- (acetoxy-methyl) -1- ((4- acetyloxy phenyls) acetenyl) -9H- fluorenes -9,9- two takes
Generation) two (4,1- phenylenes) diacetate esters or derivatives thereof.
In addition, in embodiment preferred for this invention, the activity in the sunscreen cream of ultra-violet radiation resisting as described above into
Divide by (7- acetoxyl group -2- (acetoxy-methyl) -1- ((4- acetyloxy phenyls) acetenyl) replacements of -9H- fluorenes -9,9- two) two
(4,1- phenylenes) diacetate esters are prepared from as sole component.
The sunscreen cream of ultra-violet radiation resisting of the present invention, is conventional work of the those skilled in the art using this area
Skill, is prepared from by not spending on the basis of creative work.Especially, sunscreen cream of the invention can adopt following work
It is prepared by skill step：
（1）Take glycerol to mix with appropriate distilled water, when being heated to about 70 DEG C, addition sodium sulfite, ethyl hydroxybenzoate, ten
Sodium dialkyl sulfate, stirs to dissolving, makes water phase.
（2）Take methyl-silicone oil, liquid paraffin, Cera Flava, 1- hexadecanols, glyceryl monostearate, stearic acid, plus hot melt
Change, during when the temperature to about 70 DEG C, sequentially add Vitamin E, DZJB-13, make oil phase.
（3）Oil phase is slowly added in water phase in the case where being stirred continuously, emulsifying is made, is stirred to solidifying, obtain final product faint yellow oil-in-water
Type cream.
UVA and UVB wave bands in ultraviolet be human body skin produce photoaging the material impact factor, ultraviolet radiation
The generation of oxygen-derived free radicals can be increased, cause tissue oxidation resistance to reduce, ultimately result in skin tissue aging, this becomes skin and declines
Old major reason.Free radical can induce biomembrane unsaturated fatty acid --- arachidonic acid peroxidating, generate the lipids such as MDA
The metabolite of peroxide, the rising of its content can reflect the increase of skin injury degree.The antioxidation such as SOD, CAT, GSH-Px
Active enzyme material can adjust the generation of skin free radical, remove unnecessary oxygen-derived free radicals, it is to avoid too early skin aging.Collagen
Fiber is the important component in corium, predominantly collagen protein, and its content can be decreased obviously with the growth at age, so as to cause
Skin elasticity declines, cutis laxa, therefore, the degree of the change reflection skin aging of collagen content.Hyp is that intradermal content is steady
Fixed aminoacid, its content directly reflects the changes of contents of intradermal collagen fiber, so as to point out the degree of corium aging.Skin
Hyp changes of contents is the sensitive indicator for judging skin aging degree in skin tissue.
The present inventor is had found by the Mice Photoaging model investigation caused by ultraviolet, after ultraviolet radiation,
Model control group compares with Normal group, and two groups of skin histology SOD, GSH-Px, CAT, MDA levels and Hyp contents have
Have pole significant difference (P＜ 0.01)；Compare with model control group, DZJB-13 group skin histology SOD, GSH-Px, CAT levels
With Hyp contents be remarkably decreased (P＜ 0.05 orP＜ 0.01), MDA levels significantly raise (P＜ 0. is 01).This imply that DZJB-
13 oxidation resistances that can improve ultraviolet radiation skin, accelerate scavenging activated oxygen, and adjust skin after ultraviolet radiation
Tissue amino acid metabolism, increases the content of skin collagen, makees so as to play protection to ultraviolet irradiation hairless mouse skin
With delaying skin photoaging.Therefore, the present invention also provides a kind of compound new application, i.e.,：(7- acetoxyl group -2- (acetyl oxygen
Ylmethyl) -1- ((4- acetyloxy phenyls) acetenyl) replacements of -9H- fluorenes -9,9- two) two (4,1- phenylenes) diacetate esters or its
Application of the derivant in the skin care item for preparing ultra-violet radiation resisting.
Compared with prior art, the invention provides (7- acetoxyl group -2- (acetoxy-methyl) -1- ((4- acetyl oxygen
Base benzene) acetenyl) replacements of -9H- fluorenes -9,9- two) two (4,1- phenylenes) diacetate esters and its derivant be used to prepare uvioresistant
The sunscreen cream of beta radiation, the sunscreen cream can improve the oxidation resistance of ultraviolet radiation skin, accelerate scavenging activated oxygen, and
Skin histology amino acid metabolism after ultraviolet radiation is adjusted, increases the content of skin collagen, so as to irradiate skin to ultraviolet
Skin plays protective action, delaying skin photoaging.
Specific embodiment
The preparation embodiment and effete test embodiment of sunscreen cream of the present invention is the following is, the description of these embodiments is for ease of this
The those of ordinary skill of technical field is understood that and applies the present invention.Person skilled in the art obviously can be easily right
These embodiments make various modifications, and General Principle described herein is applied in other embodiment without through creating
The work of property.Therefore, protection scope of the present invention is not limited to these embodiments, and those skilled in the art are of the invention to take off
Show, the improvement and modification made without departing from scope all should be within protection scope of the present invention.
Embodiment 1：The preparation of DZJB-13 cream
Prescription：Vitamin E 3g, methyl-silicone oil 50g, liquid paraffin 80g, Cera Flava 7.5g, 1- hexadecanol 40g, stearic acid
50g, glyceryl monostearate 20g, DZJB-13 15g, glycerol 100g, sodium sulfite 3g, ethyl hydroxybenzoate 1g, dodecyl
Sodium sulfate 5g, distilled water adds to full dose 1000g.
Preparation technology：
（1）Take glycerol to mix with appropriate distilled water, when being heated to about 70 DEG C, addition sodium sulfite, ethyl hydroxybenzoate, ten
Sodium dialkyl sulfate, stirs to dissolving, makes water phase.
（2）Take methyl-silicone oil, liquid paraffin, Cera Flava, 1- hexadecanols, glyceryl monostearate, stearic acid, plus hot melt
Change, during when the temperature to about 70 DEG C, sequentially add Vitamin E, DZJB-13, make oil phase.
（3）Oil phase is slowly added in water phase in the case where being stirred continuously, emulsifying is made, is stirred to solidifying, obtain final product faint yellow oil-in-water
Type cream.
Quality control：Prepared DZJB-13 cream is homogeneous, and denseness is suitable, and stretchability is good.Jing centrifugal tests and resistance to
Hot low temperature resistant test, without lamination, without oil-water separation phenomenon, outward appearance is also without significant change for cream.In room temperature 6 months, Jing
The projects such as outward appearance, color and luster, denseness, uniformity, pH value, the content of observation cream have no significant change.
Comparative example 1：The preparation of blank cream
Except being added without DZJB-13 in step 2, other steps and supplementary product kind and consumption are with embodiment 1.
Embodiment 2：DZJB-13 cream is studied the controlling experiment of Mice Photoaging model
Kunming kind healthy mice 36 is chosen, body weight 20-25g, female is randomly divided into following three groups：Normal group, mould
Type matched group and DZJB-13 groups, 12 per group.Mice dorsal body setae is shaved only, area 2cm × 2cm.In addition to Normal group, its with
(UVA and UVB ultraviolet lamp tubes are produced two groups of mices by Hefei Sai Fan testing equipments company limited, model UVA- under uviol lamp
340 and UVB-313 types) simulation sun exposure, irradiation distance 50cm, 1h/d, 5 times a week, continuous 8 weeks.Ultraviolet irradiates
Before 20min, the DZJB-13 cream that DZJB-13 groups mice prepares in hair removal section uniform application embodiment 1, Normal group and mould
Type matched group smears the blank cream of the preparation of comparative example 1.After off-test, every animal UV-irradiating portion skin is taken complete
Layer 1g, prepares skin histology homogenate, and MDA, SOD, GSH-Px, CAT, Hyp level is detected respectively.In addition, taking ultraviolet irradiation
Area skin 0.3cm × 0.3cm, 10% formalin is fixed, paraffin embedding, section, HE dyeing, using observation by light microscope
Skin histopathology's form.
UV-irradiating portion skin histology SOD, GSH-Px, CAT and MDA content statistical result is shown in Table 1.By table 1
Result of the test can be seen that model control group and Normal group this two groups of skin histologies SOD, GSH-Px, CAT and MDA
Level be respectively provided with pole significant difference (P＜ 0.01)；Compare with model control group, DZJB-13 group skin histology SOD, GSH-Px,
CAT levels be remarkably decreased (P＜ 0.05 orP＜ 0.01), MDA levels significantly raise (P＜ 0. is 01).
Each group mice skin tissue SOD, GSH-Px, the CAT and MDA comparision contents of table 1
| Group | n | SOD（μmol/g） | GSH-Px（μmol/g） | CAT（μmol/g） | MDA（μmol/g） |
| Normal group | 12 | 138.5±15.3** | 128.0±13.6** | 319.6±32.5** | 21.7±2.8** |
| Model control group | 12 | 101.3±12.8 | 96.6±11.3 | 255.8±26.0 | 29.0±3.5 |
| DZJB-13 groups | 12 | 125.0±14.9* | 121.7±10.5** | 304.7±31.3* | 20.2±2.6** |
Compare with model control group,* P＜ 0.05,** P＜ 0.01.
UV-irradiating portion skin histology Hyp content statistical results are shown in Table 2.Can be seen by the result of the test of table 2
Go out, model control group compares with Normal group, two groups of skin histology Hyp contents have pole significant difference (P＜ 0.01)；With
Model control group compares, DZJB-13 group skin histology Hyp contents be remarkably decreased (P＜ 0.01).
The each group mice skin tissue Hyp comparision contents of table 2
| Group | n | Hyp contents（mg/g） |
| Normal group | 12 | 3.42±0.50** |
| Model control group | 12 | 2.51±0.33 |
| DZJB-13 groups | 12 | 3.27±0.48** |
Compare with model control group,* P＜ 0.05,** P＜ 0.01.
In addition, observation shows under skin texture optical microscope (10 × 40), model control group epidermis is substantially thickened,
Organizational structure is imperfect, fibrous tissue arrangement disorder, phenomenon of rupture occurs, it is seen that inflammatory cell infiltration, sebaceous gland irregularly increases
It is raw.Normal group and DZJB-13 group horny layer are thin, epidermal tissue's structural integrity, and each confluent monolayer cells are clear, skin corium fibrous tissue
It is uniformly distributed in wavy.
Claims (1)
1. (7- acetoxyl group -2- (acetoxy-methyl) -1- ((4- acetyloxy phenyls) acetenyl) replacements of -9H- fluorenes -9,9- two)
Two (4,1- phenylenes) applications of diacetate esters in the skin care item for preparing ultra-violet radiation resisting.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510064458.8A CN104622722B (en) | 2015-02-09 | 2015-02-09 | Anti-ultraviolet radiation sun cream |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510064458.8A CN104622722B (en) | 2015-02-09 | 2015-02-09 | Anti-ultraviolet radiation sun cream |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104622722A CN104622722A (en) | 2015-05-20 |
| CN104622722B true CN104622722B (en) | 2017-04-19 |
Family
ID=53202291
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510064458.8A Active CN104622722B (en) | 2015-02-09 | 2015-02-09 | Anti-ultraviolet radiation sun cream |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104622722B (en) |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103588616A (en) * | 2013-10-18 | 2014-02-19 | 中山大学 | Selaginella pulvinata extract as well as preparation method and applications thereof |
-
2015
- 2015-02-09 CN CN201510064458.8A patent/CN104622722B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103588616A (en) * | 2013-10-18 | 2014-02-19 | 中山大学 | Selaginella pulvinata extract as well as preparation method and applications thereof |
Non-Patent Citations (3)
| Title |
|---|
| 卷柏属植物化学成分及药理活性研究进展;邹辉等;《天然产物研究与开发》;20121231;第24卷;第1655-1670页 * |
| 卷柏属药用植物生物学活性研究进展;张艺轩等;《中成药》;20110430;第33卷(第4期);第664-668页 * |
| 基质金属蛋白酶与皮肤光老化;郑锦芬等;《中华医学美学美容杂志》;20120229;第18卷(第1期);第75-77页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104622722A (en) | 2015-05-20 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Ichihashi et al. | Preventive effect of antioxidant on ultraviolet-induced skin cancer in mice | |
| CN105102073B (en) | Part lightening composition and its application method | |
| CN106176259B (en) | Sunscreen cream with double isolation and after-sun repair effects | |
| JPH10291929A (en) | Wrinkle suppressive preparation for external use for skin | |
| CN107638331A (en) | A kind of maintenance skin barrier and the moisturizing emulsion for resisting free radical | |
| CN108030718A (en) | A kind of sun-proof whitening and skin-protecting composition and preparation method thereof | |
| CN111067838A (en) | Whitening and freckle-removing composition, whitening and freckle-removing cream and preparation method thereof | |
| Kamel et al. | Diosmin/essential oil combination for dermal photo-protection using a lipoid colloidal carrier | |
| CN106619227B (en) | Compound grease, using suncream of the compound grease and preparation method thereof | |
| JP2018162325A (en) | Cosmetic compositions comprising marine plants | |
| CN111714400A (en) | Cosmetic with anti-aging and repairing effects and preparation method thereof | |
| US10493018B1 (en) | Topical moisturizing composition | |
| CN110547991A (en) | aloe whitening and freckle removing cream and preparation method thereof | |
| KR101856480B1 (en) | Cosmetic composition with microalgae extract for anti-UV and skin-irritation alleviation effect | |
| KR20150025680A (en) | EXTERNAL APPLICATING COMPOITION COMPRISING OF PPARΑ/γ DUAL ACTIVATOR FOR INHIBITING AND IMPROVING OF SKIN AGING | |
| KR20130024316A (en) | Cosmetic composition for improving skin condition comprising growth factor and cytokine induced human placenta | |
| CN104622722B (en) | Anti-ultraviolet radiation sun cream | |
| CN111632018A (en) | Composition containing hydrolyzed algae extract and application thereof | |
| JPH08337510A (en) | Melanin formation inhibitor | |
| CN102258434A (en) | Application of polygonin in preparing sunscreen cosmetics | |
| CN103491943B (en) | Comprise the compositions (aging) of Ficus microcarpa Linn. f, nelumbium and Herba Trifolii Pratentis serum fraction | |
| KR20200021709A (en) | Melanocyte migration promoting composition comprising asparagus extract | |
| JP2004331602A (en) | Skin care preparation for external use | |
| RU2660359C2 (en) | Cosmetic or dermatological compositions combining retinaldehyde and glycylglycine oleamide and cosmetic or dermatological uses thereof | |
| CN108969465B (en) | Refreshing, relaxing and isolating sun block and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| CB03 | Change of inventor or designer information | ||
| CB03 | Change of inventor or designer information |
Inventor after: Zhang Fengjiao Inventor before: Li Jian |
|
| TA01 | Transfer of patent application right | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20170315 Address after: High tech Industrial Park Road 510900 Hong Kong Guangdong Conghua Economic Development Zone No. 22 building 3 Applicant after: Guangdong Chaocai Biological Technology Co.,Ltd. Address before: Jinghu District of Anhui city of Wuhu province Jiuhua Road 241000 No. 263 in the Department of Dermatology of Wuhu Second People's Hospital Applicant before: Li Jian |
|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20200421 Address after: 510000 room 105, building 1, zone a, green headquarters center, Zhongjin Road, East Industrial Park, Lishui Town, Nanhai District, Foshan City, Guangdong Province Patentee after: Guangdong Mishang Biotechnology Co.,Ltd. Address before: High tech Industrial Park Road 510900 Hong Kong Guangdong Conghua Economic Development Zone No. 22 building 3 Patentee before: Guangdong Chaocai Biological Technology Co.,Ltd. |