A kind of sunscreen cream of ultra-violet radiation resisting
Technical field
The invention belongs to technical field of skin care, in particular to a kind of sunscreen cream of ultra-violet radiation resisting.
Background technology
Along with ozone layer is day by day thinning, the ultraviolet arriving ground is on the increase.The uitraviolet intensity contained in sunlight is very high, summer direct sunlight skin, ultraviolet easy penetrating skin surface, ultraviolet radiation can not only produce melanin, makes skin darkening, makes the possibility of skin ageing in addition, after skin accepts ultraviolet overexposure, can lesional epidermis cell.The damage great majority that sunlight causes are that the ultraviolet radiation being 200 to 400nm by wavelength causes.The UVC that its medium wavelength is less than 290nm is absorbed by ozone layer substantially, cannot arrive ground; Wave-length coverage is that the UVB of 290 to 320nm can cause burning and cutaneous pigmentation; Wave-length coverage is that the UVA of 320 to 400nm can cause slightly burning with tanned.The sun-prevention component used in usual cosmetics, as nano titanium oxide, zinc oxide, benzophenone etc., owing to there is such as skin irritation, problem such as blocking pore, heliosensitivity etc., has strict consumption restriction.The sunscreen of natural botanical source, because of its wide spectrum, safe sun-proof result, enjoys consumers.
CN103588616A discloses a kind of Selaginella pulvinata extract and its preparation method and application, conduct in-depth research for Selaginella pulvinata extract, use multiple column chromatography separation technology and half preparative high-performance liquid chromatographic separation method, extraction and isolation has 9 to a class, the compound (DZJB1-13) of the new framing structure of 9-diphenyl-1-(phenylacetylene base)-9H-fluorenes, and disclose the activity that these compounds significantly can suppress 4 type phosphodiesterases (PDE4), can be used for preparation PDE4 inhibitor, treatment asthma, chronic obstructive pneumonia, inflammation etc.At present, the biological activity after still not having this compounds external of bibliographical information in the skin injury that causes of control ultraviolet.
Summary of the invention
The object of the invention is to, by studying the biological activity of native compound monomer, provide a kind of sunscreen cream of ultra-violet radiation resisting.This sunscreen cream is with (7-acetoxyl group-2-(acetoxy-methyl)-1-((4-acetyloxy phenyl) acetenyl)-9H-fluorenes-9,9-bis-replaces) two (4,1-phenylene) diacetate esters (DZJB-13) or derivatives thereof is active component, Mice Photoaging model caused by ultraviolet is had to the protective action of highly significant, can be developed into the sunscreen cream of ultra-violet radiation resisting.
In order to realize object of the present invention, inventor is studied and persistent exploration by a large amount of animal experiment, finally obtains following technical scheme:
A kind of sunscreen cream of ultra-violet radiation resisting, this sunscreen cream is prepared from by active component and adjuvant, described active component comprises (7-acetoxyl group-2-(acetoxy-methyl)-1-((4-acetyloxy phenyl) acetenyl)-9H-fluorenes-9,9-bis-replaces) two (4,1-phenylene) diacetate esters or derivatives thereof.
In addition, in embodiment preferred for this invention, active component in the sunscreen cream of ultra-violet radiation resisting described above is by (7-acetoxyl group-2-(acetoxy-methyl)-1-((4-acetyloxy phenyl) acetenyl)-9H-fluorenes-9,9-bis-replaces) two (4,1-phenylene) diacetate esters is prepared from as sole component.
The sunscreen cream of ultra-violet radiation resisting of the present invention, is the common process that those skilled in the art utilize this area, the basis not spending creative work can be prepared from.Especially, sunscreen cream of the present invention can adopt following processing step to prepare:
(1) get glycerol to mix with appropriate distilled water, when being heated to about 70 DEG C, adding sodium sulfite, ethyl hydroxybenzoate, sodium lauryl sulphate, be stirred to dissolving, make aqueous phase.
(2) get methyl-silicone oil, liquid paraffin, Cera Flava, 1-hexadecanol, glyceryl monostearate, stearic acid, heat fused, during when the temperature to about 70 DEG C, add vitamin E, DZJB-13 successively, make oil phase.
(3) oil phase is slowly added in aqueous phase under constantly stirring, make emulsifying, be stirred to solidifying, obtain faint yellow oil-in-water type cream.
UVA and UVB wave band in ultraviolet is the material impact factor that human body skin produces photoaging, and ultraviolet radiation can increase the generation of oxygen-derived free radicals, causes and organizes oxidation resistance to reduce, and finally causes skin tissue aging, and this becomes the major reason of skin aging.Free radical can bring out biomembrane unsaturated fatty acid---and arachidonic acid peroxidating, generate the metabolite of the lipid peroxide such as MDA, the rising of its content can reflect the increase of skin injury degree.The antioxidant activity enzyme materials such as SOD, CAT, GSH-Px can the generation of regulation of skin free radical, removes unnecessary oxygen-derived free radicals, avoids too early skin aging.Collagen fiber are the important component in corium, are mainly collagen protein, and its content obviously can decline with the growth at age, thus cause skin elasticity to decline, cutis laxa, therefore, and the degree of the change reflection skin aging of collagen content.Hyp is the aminoacid of intradermal stable content, and its content directly reflects the changes of contents of intradermal collagen fiber, thus the degree of prompting corium aging.In skin histology, Hyp changes of contents is the sensitive indicator judging skin aging degree.
The present inventor is found by the Mice Photoaging model investigation caused by ultraviolet, after ultraviolet radiation, model control group compares with Normal group, two groups of skin histology SOD, GSH-Px, CAT, MDA levels and Hyp content all have pole significant difference (
p< 0.01); Compare with model control group, DZJB-13 group skin histology SOD, GSH-Px, CAT level and Hyp content significantly decline (
p< 0.05 or
p< 0.01), MDA level significantly raises (
p< 0. 01).This imply that DZJB-13 can improve the oxidation resistance of ultraviolet radiation skin, accelerate scavenging activated oxygen, and skin histology amino acid metabolism after regulating ultraviolet radiation, increase the content of skin collagen, thus protective action is played to ultraviolet radiation hairless mouse skin, delaying skin photoaging.Therefore, the present invention also provides a kind of compound novelty teabag, that is: (7-acetoxyl group-2-(acetoxy-methyl)-1-((4-acetyloxy phenyl) acetenyl)-9H-fluorenes-9,9-bis-replaces) two application of (4,1-phenylene) diacetate esters or derivatives thereof in the skin care item preparing ultra-violet radiation resisting.
Compared with prior art, the invention provides (7-acetoxyl group-2-(acetoxy-methyl)-1-((4-acetyloxy phenyl) acetenyl)-9H-fluorenes-9, 9-bis-replaces) two (4, 1-phenylene) diacetate esters and derivant thereof be for the preparation of the sunscreen cream of ultra-violet radiation resisting, this sunscreen cream can improve the oxidation resistance of ultraviolet radiation skin, accelerate scavenging activated oxygen, and skin histology amino acid metabolism after regulating ultraviolet radiation, increase the content of skin collagen, thus protective action is played to ultraviolet radiation skin, delaying skin photoaging.
Detailed description of the invention
Be below preparation embodiment and the effect test example of sunscreen cream of the present invention, the description of these embodiments can understand and apply the invention for ease of those skilled in the art.Person skilled in the art obviously easily can make various amendment to these embodiments, and General Principle described herein is applied in other embodiments and need not through performing creative labour.Therefore, protection scope of the present invention is not limited to these embodiments, and those skilled in the art, according to announcement of the present invention, do not depart from improvement that scope makes and amendment all should within protection scope of the present invention.
the preparation of embodiment 1:DZJB-13 cream
Prescription: vitamin E 3g, methyl-silicone oil 50g, liquid paraffin 80g, Cera Flava 7.5g, 1-hexadecanol 40g, stearic acid 50g, glyceryl monostearate 20g, DZJB-13 15g, glycerol 100g, sodium sulfite 3g, ethyl hydroxybenzoate 1g, sodium lauryl sulphate 5g, distilled water is added into full dose 1000g.
Preparation technology:
(1) get glycerol to mix with appropriate distilled water, when being heated to about 70 DEG C, adding sodium sulfite, ethyl hydroxybenzoate, sodium lauryl sulphate, be stirred to dissolving, make aqueous phase.
(2) get methyl-silicone oil, liquid paraffin, Cera Flava, 1-hexadecanol, glyceryl monostearate, stearic acid, heat fused, during when the temperature to about 70 DEG C, add vitamin E, DZJB-13 successively, make oil phase.
(3) oil phase is slowly added in aqueous phase under constantly stirring, make emulsifying, be stirred to solidifying, obtain faint yellow oil-in-water type cream.
Quality control: prepared DZJB-13 cream is homogeneous, denseness is suitable for, and stretchability is good.Through centrifugal test and heat-resisting low temperature resistant test, cream is without lamination, and without oil-water separation phenomenon, outward appearance is also without significant change.Place 6 months in room temperature, the project such as outward appearance, color and luster, denseness, uniformity, pH value, content through observing cream has no significant change.
comparative example 1: the preparation of blank cream
Except not adding DZJB-13 in step 2, other steps and supplementary product kind and consumption are all with embodiment 1.
embodiment 2:DZJB-13 cream is studied the controlling experiment of Mice Photoaging model
Choose Kunming kind healthy mice 36, body weight 20-25g, female, be divided into following three groups at random: Normal group, model control group and DZJB-13 group, often organize 12.Mice dorsal body setae is shaved only, area 2cm × 2cm.Except Normal group, itself and two groups of mices (UVA and UVB ultraviolet lamp tube is produced by Hefei Sai Fan testing equipment company limited, model UVA-340 and UVB-313 type) solar simulated under uviol lamp are irradiated, irradiation distance 50cm, 1h/d, 5 times weekly, continuous 8 weeks.Before ultraviolet radiation 20min, the DZJB-13 cream that DZJB-13 group mice is prepared in depilation district uniform application embodiment 1, Normal group and model control group all smear blank cream prepared by comparative example 1.After off-test, get every animal UV-irradiating portion skin holostrome 1g, prepare skin histology homogenate, detect MDA, SOD, GSH-Px, CAT, Hyp level respectively.In addition, get UV-irradiating portion skin 0.3cm × 0.3cm, 10% formalin is fixed, and paraffin embedding, section, HE dyeing, adopt observation by light microscope skin histopathology form.
The statistical result of UV-irradiating portion skin histology SOD, GSH-Px, CAT and MDA content is in table 1.Can be found out by the result of the test of table 1, model control group and these two groups of skin histology SOD, GSH-Px, CAT and MDA levels of Normal group all have pole significant difference (
p< 0.01); Compare with model control group, DZJB-13 group skin histology SOD, GSH-Px, CAT level significantly declines (
p< 0.05 or
p< 0.01), MDA level significantly raises (
p< 0. 01).
Table 1 is group mice skin tissue SOD, GSH-Px, CAT and MDA comparision contents respectively
Group |
n |
SOD(μmol/g) |
GSH-Px(μmol/g) |
CAT(μmol/g) |
MDA(μmol/g) |
Normal group |
12 |
138.5±15.3
** |
128.0±13.6
** |
319.6±32.5
** |
21.7±2.8
** |
Model control group |
12 |
101.3±12.8 |
96.6±11.3 |
255.8±26.0 |
29.0±3.5 |
DZJB-13 group |
12 |
125.0±14.9
* |
121.7±10.5
** |
304.7±31.3
* |
20.2±2.6
** |
Compare with model control group,
* p< 0.05,
* p< 0.01.
The statistical result of UV-irradiating portion skin histology Hyp content is in table 2.Can be found out by the result of the test of table 2, model control group compares with Normal group, two groups of skin histology Hyp content have pole significant difference (
p< 0.01); Compare with model control group, DZJB-13 group skin histology Hyp content significantly declines (
p< 0.01).
Table 2 is group mice skin tissue Hyp comparision contents respectively
Group |
n |
Hyp content (mg/g) |
Normal group |
12 |
3.42±0.50
** |
Model control group |
12 |
2.51±0.33 |
DZJB-13 group |
12 |
3.27±0.48
** |
Compare with model control group,
* p< 0.05,
* p< 0.01.
In addition, observe display under skin texture optical microscope (10 × 40), model control group epidermis obviously thickens, and organizational structure is imperfect, and phenomenon of rupture, appears in fibrous tissue arrangement disorder, visible inflammatory cell infiltration, the irregular hypertrophy of sebaceous gland.Normal group and DZJB-13 group horny layer thin, epidermal tissue's structural integrity, each confluent monolayer cells is clear, and skin corium fibrous tissue is wavy being uniformly distributed.