CN105982828A - Alpinia oxyphylla essential oil for protecting skin, as well as preparation method and application thereof - Google Patents

Alpinia oxyphylla essential oil for protecting skin, as well as preparation method and application thereof Download PDF

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Publication number
CN105982828A
CN105982828A CN201610571460.9A CN201610571460A CN105982828A CN 105982828 A CN105982828 A CN 105982828A CN 201610571460 A CN201610571460 A CN 201610571460A CN 105982828 A CN105982828 A CN 105982828A
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essential oil
intelligence development
heal
preparation
oil
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CN105982828B (en
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孙愉
龚忠勤
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Shenzhen Sino English Technology Co Ltd
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Shenzhen Sino English Technology Co Ltd
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/92—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof
    • A61K8/922—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof of vegetable origin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
    • A61K8/34—Alcohols
    • A61K8/345—Alcohols containing more than one hydroxy group
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/67—Vitamins
    • A61K8/678—Tocopherol, i.e. vitamin E
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
    • A61Q17/005—Antimicrobial preparations
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/08—Anti-ageing preparations
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
    • A61K2800/59—Mixtures
    • A61K2800/592—Mixtures of compounds complementing their respective functions
    • A61K2800/5922—At least two compounds being classified in the same subclass of A61K8/18

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Dermatology (AREA)
  • Birds (AREA)
  • Epidemiology (AREA)
  • Gerontology & Geriatric Medicine (AREA)
  • Emergency Medicine (AREA)
  • Chemical & Material Sciences (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Cosmetics (AREA)

Abstract

The invention belongs to the technical field of skin-protecting cosmetics, and particularly discloses alpinia oxyphylla essential oil for protecting skin, as well as a preparation method and application thereof. The essential oil is prepared from the following components in percentage by mass: 10-20 percent of alpinia oxyphylla shell essential oil, 60-70 percent of fructus alpiniae oxyphyllae essential oil, 18-22 percent of glycerol and 2-8 percent of vitamin E. The essential oil is prepared from natural substances and natural herbal oil, has stable property and cannot be easily oxidized. The essential oil contains natural unsaturated fatty acid, trans-caryophyllene and other effective ingredients, has broad-spectrum antibacterial property, has an excellent sterilizing capability on various pathogenic bacteria and fungi, has good percutaneous permeability, is beneficial to skin absorption, and is used for nourishing hair follicles, repairing skin and delaying skin senescence.

Description

Intelligence development heal-care essential oil and its preparation method and application
Technical field
The present invention relates to skin protection cosmetics technical field, particularly relate to a kind of intelligence development heal-care essential oil and preparation side thereof Method and application.
Background technology
Owing to skin is the first line of defence that human body resists the invasions such as harmful substance, bacterium, virus so that it is become For being most susceptible to outside world and most frequent by outside world and that damage is most organ.Meanwhile, skin is also It is the important component part embodying the external aesthetic feeling of human body and feature.Therefore a good local is created to skin Environment, is conducive to the injury repair of profit skin, delay skin aging and the formation reducing wrinkle.
But, with advancing age, the ability of body removing free radical constantly declines so that free radical exists Internal accumulation, causes the MDAs such as MDA to be cross-linked to form indissoluble thing with protein, nucleic acid or lipid Matter, this improper crosslinking causes Skin Cell living environment to change so that Skin Cell structural deterioration, extremely Dying, skin will show the aging phenomenons such as dry, coarse, lax.And during skin senescence, adjoint Horn cell aging, the in poor quality that epiderm skin occurs, also cause the generation of wrinkle of skin.
Therefore, various skin care item arise at the historic moment.Substantial amounts of research shows, effect of skin protection cosmetics It is mainly reflected in: keep the moisture in skin, strengthens skin oxidation resistance, reduce the damage of Skin Cell And apoptosis.
But, the usual substantial amounts of synthetics of traditional skin protection cosmetics, solvent, excitatory to skin Greatly, it is easy to injured skin.With the raising day by day of people's living standard, pursue environmental protection, back to nature Skin care theory also strengthen therewith, the natural products therefore seeking no side effects is active additive and change Cosmetic raw material becomes the focus of skin protection cosmetics industry.Zingiber Alpinia plants intelligence development (Alpiniaoxyphylla Miq.) shell in its fruit and seed contain natural plant grease, there is low iodine number, aliphatic acid composition and people The features such as body skin is similar, its essential oil is strong to the permeability of skin simultaneously, is easily absorbed by the body, without greasy feeling, It is the desirable feedstock developing into natural skin protection cosmetics.
Content of the invention
The present invention is directed to that traditional skin care cosmetics exist due to right containing a large amount of syntheticses, solvent etc. Skin irritation is big, be easy to the problems such as injured skin, provides a kind of intelligence development heal-care essential oil；And this intelligence development protects The preparation method and application of skin essential oil.
To achieve the above object of the invention, the embodiment of the present invention have employed following technical scheme:
A kind of intelligence development heal-care essential oil, including the component that mass percent is following:
And, the preparation method of a kind of intelligence development heal-care essential oil, at least comprise the following steps:
Intelligence development heal-care essential oil weighs vitamin E, glycerine, intelligence development shell essential oil and fructus alpiniae oxyphyllae essence as described above Oil；
The described vitamin E weighing, glycerine are carried out mixing treatment, obtains the first solution；
The described intelligence development shell essential oil weighing, fructus alpiniae oxyphyllae essential oil are heated, and add in the first solution, Mixing treatment.
Correspondingly, application in cosmetics for the above-mentioned intelligence development heal-care essential oil.
The intelligence development heal-care essential oil of the above embodiment of the present invention uses intelligence development shell essential oil and fructus alpiniae oxyphyllae essential oil as mainly Composition, in conjunction with glycerine and vitamin E, becomes and belongs to natural materials, crude vegetal, stable in properties, no Easily oxidized；And containing active ingredients such as natural unrighted acid, cloves alkene；There is spectrum antibacterial, right A lot of malignant bacterias and fungi have good sterilizing ability；Good to percutaneous permeability, beneficially skin absorption, Moisten hair follicle, there is repair to skin, the aging of delaying skin.
The preparation method of intelligence development heal-care essential oil that the embodiment of the present invention provides, raw material sources extensively, be not necessarily to Adding other auxiliary agents, processing technology is simple, it is thus achieved that intelligence development heal-care essential oil stable performance, without lamination.
The intelligence development heal-care essential oil that the embodiment of the present invention provides is used in cosmetics, and stable in properties is difficult to oxidized； There is spectrum antibacterial, good sterilizing ability is had to a lot of malignant bacterias and fungi；To percutaneous permeability Good, beneficially skin absorption, moisten hair follicle, there is to skin repair, can effective the declining of delaying skin Always.
Detailed description of the invention
In order to make the purpose of the present invention, technical scheme and advantage clearer, below in conjunction with embodiment, The present invention is further elaborated.It should be appreciated that specific embodiment described herein only in order to Explain the present invention, be not intended to limit the present invention.
The embodiment of the present invention provides a kind of intelligence development heal-care essential oil, including the component that mass percent is following:
In a preferred embodiment, in intelligence development heal-care essential oil, the quality of intelligence development shell essential oil and fructus alpiniae oxyphyllae essential oil Proportioning is 1:3～1:4, and this optimum ratio can effectively improve anti-skin compared with being used alone with intelligence development shell essential oil Old and feeble effect.
In a preferred embodiment, intelligence development shell essential oil is prepared by following methods:
It is placed in the intelligence development shell powder less than or equal to 400 microns for the granularity in volatile oil extractor, use water to steam At least 8h is extracted in steam distillation, obtains volatile oil；
The volatile oil of above-mentioned acquisition is placed in 0～4 DEG C of environment standing, filters, obtain oil reservoir；
The oil reservoir of above-mentioned acquisition is placed in the water-bath of 70～99 DEG C or at least 6h is extracted in oil bath distillation, then carry Take cut, the intelligence development shell essential oil required for i.e. can get after cooled process.
In a preferred embodiment, described fructus alpiniae oxyphyllae essential oil is prepared by following methods:
It is placed in the Sharpleaf Glangal Fruit less than or equal to 400 microns for the granularity in volatile oil extractor, use water to steam At least 8h is extracted in steam distillation, obtains volatile oil；
The volatile oil of above-mentioned acquisition is placed in 0～4 DEG C of environment standing, filters, obtain oil reservoir；
The oil reservoir of above-mentioned acquisition is placed in the water-bath of 70～99 DEG C or at least 6h is extracted in oil bath distillation, then carry Take cut, the fructus alpiniae oxyphyllae essential oil required for i.e. can get after cooled process.
The intelligence development heal-care essential oil that the above embodiment of the present invention provides, uses intelligence development shell essential oil and fructus alpiniae oxyphyllae essential oil to make For main component, in conjunction with glycerine and vitamin E, all the components belongs to natural materials, crude vegetal, property Matter is stable, is difficult to oxidized；And containing active ingredients such as natural unrighted acid, cloves alkene；There is spectrum A lot of malignant bacterias and fungi are had good sterilizing ability by antibiotic property；Good, favorably to percutaneous permeability In skin absorption, moisten hair follicle, there is repair to skin, the aging of delaying skin.
Correspondingly, the present invention is on the basis of above-described embodiment, it is further provided embodiment of the present invention intelligence development The preparation method of heal-care essential oil.In one embodiment, the preparation method of described intelligence development heal-care essential oil at least includes Following steps:
Intelligence development heal-care essential oil weighs vitamin E, glycerine, intelligence development shell essential oil and fructus alpiniae oxyphyllae essence as described above Oil；
The described vitamin E weighing, glycerine are carried out mixing treatment, obtains the first solution；
The described intelligence development shell essential oil weighing, fructus alpiniae oxyphyllae essential oil are heated, is subsequently adding the first solution In, mixing treatment.
Preferably, described intelligence development shell essential oil, fructus alpiniae oxyphyllae essential oil are heated to 35 DEG C～45 DEG C, then while hot slowly Add in the first solution, by improving described intelligence development shell essential oil, the temperature of fructus alpiniae oxyphyllae essential oil, to improve and the The intersolubility of one solution.
Preferably, the preparation method of above-mentioned intelligence development heal-care essential oil also includes described intelligence development shell essential oil, fructus alpiniae oxyphyllae essence The preparation method of oil.It is specific as follows,
Granularity is less than or equal to step S01. the intelligence development shell powder of 400 microns or Sharpleaf Glangal Fruit distills, Obtain volatile oil；
Described volatile oil is placed in 0～4 DEG C of environment standing, filters by step S02., obtains oil reservoir；
Described oil reservoir is distilled in 70～99 DEG C by step S03., takes cut.
It is further preferred that the extraction of intelligence development shell powder or Sharpleaf Glangal Fruit is by intelligence development shell powder or fructus alpiniae oxyphyllae Powder is placed in volatile oil extractor, uses steam distillation to extract 8～10h.
Preferably, being placed in the oil reservoir of above-mentioned acquisition in 70～99 DEG C the mode distilled is water-bath or oil bath Distillation, distillation extraction at least 6～8h, then extract cut, required for i.e. can get after cooled process Intelligence development shell essential oil.
The preparation method of intelligence development heal-care essential oil that the above embodiment of the present invention provides, raw material sources extensively, Without adding other auxiliary agents, processing technology is simple, it is thus achieved that intelligence development heal-care essential oil stable performance, existing without layering As.
Either the intelligence development heal-care essential oil of above-mentioned offer still has intelligence development heal-care essential oil prepared by above-mentioned preparation method, All can apply in the raw material of cosmetics, as being used for preparing anti aging effect, moisturizing, conditioning skin etc. Cosmetics.When being used for described intelligence development heal-care essential oil in cosmetics, due to the character of intelligence development heal-care essential oil Stable, it is difficult to oxidized；There is spectrum antibacterial, have good bacterium energy to a lot of malignant bacterias and fungi Power；Good to percutaneous permeability, beneficially skin absorption, moistens hair follicle, has repair, energy to skin The aging of effective delaying skin.
For intelligence development heal-care essential oil better illustrating embodiment of the present invention offer and preparation method thereof, lead to below Cross embodiment to be illustrated.
Embodiment 1
A kind of intelligence development heal-care essential oil, including following component:
The preparation method of the intelligence development heal-care essential oil that embodiment 1 provides is as follows:
The glycerine of 4g vitamin E and 16mL is mixed, stirs, obtain the first solution；
Will warm up the 30mL intelligence development shell essential oil of 40 DEG C, 50mL fructus alpiniae oxyphyllae essential oil is slowly added into the first solution Middle stirring, cooling, and with filling and sealing, obtain intelligence development heal-care essential oil.
Wherein, a kind of preparation method of intelligence development shell essential oil is as follows:
Take sun-dried intelligence development shell 2kg, pulverize, cross 40 mesh sieves, obtain intelligence development shell powder；
Intelligence development shell powder is put in volatile oil extractor, adds deionized water 6kg, use steam distillation Extract 8h, branch vibration layer, obtain 48.84mL volatile oil；
The volatile oil of acquisition is put 2 DEG C of stand at low temperature, filters, obtain 42.50mL oil reservoir；80 DEG C of water-baths Lower distillation 6h, point takes cut, cooling, obtains that 37.04mL is yellowish or colourless intelligence development shell essential oil.
A kind of preparation method of fructus alpiniae oxyphyllae essential oil is as follows:
Take sun-dried fructus alpiniae oxyphyllae 3kg, pulverize, cross 40 mesh sieves, obtain Sharpleaf Glangal Fruit；
Sharpleaf Glangal Fruit is put in volatile oil extractor, add deionized water 6kg, use steam distillation Extract 8h, branch vibration layer, obtain 58.26mL volatile oil；
The volatile oil of acquisition is put 2 DEG C of stand at low temperature, filters, obtain 25.11mL oil reservoir；80 DEG C of water-baths Lower distillation 8h, point takes cut, cooling, obtains that 23.46mL is yellowish or colourless fructus alpiniae oxyphyllae essential oil.Preparation is many Criticize fructus alpiniae oxyphyllae essential oil, i.e. can be used in embodiment 1.
In order to verify the performance of intelligence development heal-care essential oil prepared by the embodiment of the present invention 1, below by it to fell The impact of skin Keratiocyte growth carries out experimental verification.
1. experiment material
(1) application on human skin immortalization horn cell HaCat cell line, source country cell experiment resources bank；
(2) methylthiazol is blue (MTT): be purchased from Guangzhou Wei Jia company；
Intelligence development heal-care essential oil: provided by the method preparation of the embodiment of the present invention 1, preserve standby in 4 DEG C of environment With.
2. experimental technique
(1) cultivation of cell:
Application on human skin immortalization horn cell HaCat cell line is placed in containing 10% hyclone and 1% penicillin, It in the DMEM nutrient solution of streptomysin, is placed on CO2gas incubator (37 DEG C, 5%CO2, saturated humidity) In cultivate, changed nutrient solution once every 2～3 days, when cell confluency reaches 90%, with 0.25% Trypsin Induced, and carry out Secondary Culture, the good cell of phase growth conditions of taking the logarithm, for following reality Test use.
(2) intelligence development heal-care essential oil stimulates the impact of damage to application on human skin immortalization horn cell HaCat
Take the logarithm cell in growth period, with cell number 2 × 104/ mL is inoculated in 96 orifice plates, treats that cell grows to be paved with At the bottom of plate 70%～80% when, be classified as five groups, wherein the 1st group is blank group, the 2nd group～the 5th Group is separately added into the intelligence development heal-care essential oil of following various concentration: 1 μ L/L, 2 μ L/L, 4 μ L/L and 10 μ L/L. After all group cells cultivate 12h, detect cell survival rate, cell survival rate (%)=(real with mtt assay Test each cell mean/control group mean value of group) × 100%.
MTT result shows, the intelligence development heal-care essential oil group of above each concentration all has no have bright to HaCat cell Aobvious damage, is simply slightly decreased (P > 0.01) more than the cell survival rate of 4 μ L/L groups, but each with other Group compares no significant difference, illustrates that the intelligence development heal-care essential oil under this concentration is non-stimulated to Skin Cell, The results are shown in Table 1.
The survival results (n=5) of application on human skin immortalization horn cell HaCat cell respectively organized by table 1
(3) intelligence development heal-care essential oil is to H2O2The application on human skin immortalization horn cell HaCat cell survival rate of damage Impact
Take the logarithm cell in growth period, with cell number 2 × 104/ mL is inoculated in 96 orifice plates, treats that cell grows to be paved with At the bottom of plate 70%～80% when, be classified as 7 groups, wherein the 1st group is control group；2nd group is model group (H2O2400mmol/L), the 3rd group～the 6th group is variable concentrations intelligence development heal-care essential oil+400mmol/L respectively H2O2, the 3rd group～the 6th group intelligence development heal-care essential oil being separately added into following various concentration: 1 μ L/L, 2 μ L/L, 4 μ L/L and 10 μ L/L.7th group is intelligence development shell essential oil+400mmol/L H2O2, intelligence development shell essence Oil is the pure tone intelligence shell essential oil being prepared as described above method gained, and concentration is 10 μ L/L.
For the 1st group of cell, do not remake any process；
200mmol/L H is added in the 2nd group of cell2O2Solution, changes serum-free DMEM training into after 3h Supporting base, detecting cell survival rate with mtt assay, cell survival rate (%)=(this cell mean/control group is put down Average) × 100%；
Be separately added into the intelligence development heal-care essential oil cultivation 12h of variable concentrations to the 3rd group～the 6th group cell after, change DMEM culture medium for serum-free.Add 400mmol/L H2O2Solution, changes serum-free into after 3h DMEM culture medium, detects cell survival rate with mtt assay, and cell survival rate (%)=(experimental group is respectively organized flat Average/control group mean value) × 100%.
It after adding 10 μ L/L intelligence development shell essential oils to cultivate 12h in the 7th group of cell, is changed to serum-free DMEM culture medium.Add 400mmol/L H2O2Solution, changes serum-free DMEM into and cultivates after 3h Base, detects cell survival rate, cell survival rate (%)=(each cell mean/control group of experimental group with mtt assay Mean value) × 100%.
MTT result shows, compares with model group, and the intelligence development heal-care essential oil group of above each concentration can carry High cell survival rate, and in dose dependent, when the concentration of intelligence development heal-care essential oil reaches 10 μ L/L, carefully Born of the same parents' survival rate is the highest, is increased to 77.2% from 57.2%, illustrates that the intelligence development heal-care essential oil under this concentration can be notable Improve cell survival rate, and compared with alone intelligence development shell essential oil, cell survival rate is also significantly improved, knot Fruit is shown in Table 2.
Table 2 intelligence development heal-care essential oil is to H2O2The impact (n=7) of the application on human skin immortalization horn cell HaCat survival rate of damage
* representing that comparing P < 0.05, * * represents compare P < 0.01 with model group with model group, Δ represents and intelligence development Shell essential oil compares P < 0.05.
(4) intelligence development heal-care essential oil is to H2O2Application on human skin immortalization horn cell HaCat intracellular SOD of damage Impact with CAT enzyme
Phase cell of taking the logarithm inoculates 6 orifice plates, and inoculum density is 5 × 105/ hole, packet and processing method are with (3). After continuing to cultivate 24h, PBS washes 3 times, scrapes cell with cell scraper, splits on ice with RIPA lysate After solving 30min, 12000r/min centrifuges 10min, takes supernatant, and BCA method measures protein content.According to Kit explanation measures SOD, CAT enzyme activity respectively.The independent replication of this experiment 3 times, result with Represent relative to the percentage of control group, computing formula: enzyme activity=(each cell mean/control group of experimental group Mean value) × 100%, the results are shown in Table 3.
Table 3 intelligence heal-care essential oil is to H2O2Application on human skin immortalization horn cell HaCat intracellular SOD of damage and the impact (n=7) of CAT enzyme
* representing that comparing P < 0.05, * * represents compare P < 0.01 with model group with model group, Δ represents intelligence development Shell essential oil compares P < 0.05.
As shown in Table 3, comparing with model group, the intelligence development heal-care essential oil group of above each concentration can improve Cell SOD and the vigor of CAT enzyme, and in dose dependent, when the concentration of intelligence development heal-care essential oil reaches During 10 μ L/L, SOD and CAT enzyme activity improves the most obvious, respectively 80.7% and 79.9%, explanation Intelligence development heal-care essential oil under this concentration has the antioxidant effect of optimum, and compared with alone intelligence development shell essential oil, anti- Oxidation effectiveness is also significantly improved.
(5) intelligence development heal-care essential oil is to H2O2Application on human skin immortalization horn cell HaCat intracellular ROS of damage The impact of level
Phase cell of taking the logarithm inoculates 6 orifice plates, and inoculum density is 5 × 105/ hole, packet and processing method are same (3), Collecting cell after 24h, PBS washes 2 times, resuspended and to adjust cell number be 2 × 106/ mL, adds The DCFH-DA of 25 μm of ol/L hatches 30min, and PBS washes 3 times, with flow cytomery and analyze ROS positive rate, the results are shown in Table 4.
Table 4 intelligence development heal-care essential oil is to H2O2The impact (n=7) of the application on human skin immortalization horn cell HaCat intracellular ROS level of damage
* representing that comparing P < 0.05, * * represents compare P < 0.01 with model group with model group, Δ represents intelligence development Shell essential oil compares P < 0.05.
As shown in Table 4, comparing with model group, the intelligence development heal-care essential oil group of above each concentration can significantly drop The fluorescence intensity of low intracellular ROS, and in dose dependent, when the concentration of intelligence development heal-care essential oil reaches During 10 μ L/L, ROS fluorescence slightly reduces the most obvious, is down to 137.1% from 167.1%, this concentration is described Under intelligence development heal-care essential oil have the reduction response to oxidative stress effect of optimum, and compared with alone intelligence development shell essential oil, Its oxidative stress level also has significantly reduction.
The foregoing is only presently preferred embodiments of the present invention, not in order to limit the present invention, all at this Any modification, equivalent or the improvement etc. made within bright spirit and principle, should be included in the present invention Protection domain within.

Claims (10)

1. an intelligence development heal-care essential oil, including the component that mass percent is following:
2. intelligence development heal-care essential oil as claimed in claim 1, it is characterised in that: described intelligence development shell essential oil and institute The mass ratio stating fructus alpiniae oxyphyllae essential oil is 1:3～1:4.
3. a preparation method for intelligence development heal-care essential oil, at least comprises the following steps:
Weigh vitamin E, glycerine, intelligence development shell according to intelligence development heal-care essential oil as described in claim 1～2 is arbitrary Essential oil and fructus alpiniae oxyphyllae essential oil；
The described vitamin E weighing, glycerine are carried out mixing treatment, obtains the first solution；
The described intelligence development shell essential oil weighing, fructus alpiniae oxyphyllae essential oil are heated, is subsequently adding the first solution In, mixing treatment.
4. the preparation method of intelligence development heal-care essential oil as claimed in claim 3, it is characterised in that: described intelligence development After shell essential oil, fructus alpiniae oxyphyllae essential oil are heated to 35 DEG C～45 DEG C, carry out mixing treatment with described first solution while hot.
5. the preparation method of intelligence development heal-care essential oil as claimed in claim 3, also include described intelligence development shell essential oil, The preparation method of fructus alpiniae oxyphyllae essential oil, it is characterised in that:
Granularity is less than or equal to step S01. the intelligence development shell powder of 400 microns or Sharpleaf Glangal Fruit distills, Obtain volatile oil；
Described volatile oil is placed in 0～4 DEG C of environment standing, filters by step S02., obtains oil reservoir；
Described oil reservoir is distilled in 70～99 DEG C by step S03., takes cut.
6. the preparation method of intelligence development heal-care essential oil as claimed in claim 5, it is characterised in that: described step Distillation mode in S01 is steam distillation.
7. the preparation method of intelligence development heal-care essential oil as claimed in claim 5, it is characterised in that: described step Distillation mode in S03 is water-bath or oil bath distillation.
8. the preparation method of intelligence development heal-care essential oil as claimed in claim 5, it is characterised in that: described step Distillation time in S03 is 6～8h.
9. the preparation method of intelligence development heal-care essential oil as claimed in claim 3, it is characterised in that: described suddenly Distillation time in S02 is 8～10h.
10. the intelligence development heal-care essential oil as described in claim 1～2 or the intelligence development as described in claim 3～9 Intelligence development heal-care essential oil prepared by the preparation method of the heal-care essential oil application in cosmetics.
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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20190012642A (en) * 2017-07-28 2019-02-11 주식회사 코리아나화장품 Cosmetic Composition Comprising Extract of Alpinia oxyphylla Miquel As Active Ingredient
CN112544732A (en) * 2020-11-30 2021-03-26 田红远 Brain-invigorating oil

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH05178754A (en) * 1991-12-27 1993-07-20 Tsumura & Co Controlling agent for consciousness level
CN102688163A (en) * 2012-06-13 2012-09-26 中国医学科学院药用植物研究所海南分所 Anti-skin aging skin care compound essential oil

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH05178754A (en) * 1991-12-27 1993-07-20 Tsumura & Co Controlling agent for consciousness level
CN102688163A (en) * 2012-06-13 2012-09-26 中国医学科学院药用植物研究所海南分所 Anti-skin aging skin care compound essential oil

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20190012642A (en) * 2017-07-28 2019-02-11 주식회사 코리아나화장품 Cosmetic Composition Comprising Extract of Alpinia oxyphylla Miquel As Active Ingredient
KR102009201B1 (en) * 2017-07-28 2019-08-09 주식회사 코리아나화장품 Cosmetic Composition Comprising Extract of Alpinia oxyphylla Miquel As Active Ingredient
CN112544732A (en) * 2020-11-30 2021-03-26 田红远 Brain-invigorating oil

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