CN104610423B - Utilize the method for Holothurian machining blanching solution extraction glycoprotein - Google Patents
Utilize the method for Holothurian machining blanching solution extraction glycoprotein Download PDFInfo
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- CN104610423B CN104610423B CN201510061478.XA CN201510061478A CN104610423B CN 104610423 B CN104610423 B CN 104610423B CN 201510061478 A CN201510061478 A CN 201510061478A CN 104610423 B CN104610423 B CN 104610423B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/54—Improvements relating to the production of bulk chemicals using solvents, e.g. supercritical solvents or ionic liquids
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Abstract
A kind of method using Holothurian machining blanching solution extraction glycoprotein, it is characterized in that it includes the following steps:It collects sea cucumber blanching solution and is cooled to 4 10 DEG C rapidly, removing the insoluble matter in sea cucumber blanching solution using centrifugation obtains centrifuged supernatant；Centrifuged supernatant obtains desalination clear liquid using electrically-actuated device electrodialysis desalination；Desalination clear liquid uses vacuum concentration mode, is concentrated into solid content between 20 45%, obtains concentrate；Concentrate is freeze-dried or is spray-dried to obtain the first dry solid；First dry solid is utilized into petroleum ether or n-hexane or ethanol solution or supercritical CO2Fluid extraction means carry out ungrease treatment, obtain the second dry solid；Second dry solid is added in pure water, heating extraction obtains extract solution；After extraction, extract solution is removed through centrifugation and is precipitated, and supernatant collects trapped fluid by the membrane filtration of 10KDa, filtered solution ultrafiltration；Sea cucumber glycoprotein is obtained after trapped fluid drying.
Description
Technical field
The present invention relates to a kind of methods using Holothurian machining blanching solution extraction glycoprotein.
Background technology
For fresh sea cucumber after fishing, due to its own, there are the enzyme of proteolysis such as autolytic enzyme, it is easy to change skin, self-dissolving.For
Convenient for preserving, fresh sea cucumber will usually preserve after the hot water scalding treatment plus as raw material.According to incompletely statistics, 1 is often processed
Ton fresh sea cucumber can generate 0.6-1.2 tons of sea cucumber blanching solution.Along with the scale of Holothurian machining, intensive, collectable sea
It is increasingly abundanter to join blanching solution resource, how the higher value application resource, turn waste into wealth, develop into high utility value
Functional food extends sea cucumber industrial chain, is increasingly total to as scale Holothurian machining enterprise with the scientific research personnel for being engaged in sea cucumber research
With the subject of concern.
Contain a large amount of active material such as selenka in sea cucumber blanching solution（Several selenkas in imitative stichopus japonicus processing-waste
The separation identification journal of Shandong university of compound（Edition）7th phase in 2011）, sea cucumber glycoprotein（ZL 2011 1
0384722.8）Deng.There is sea cucumber glycoprotein the biology such as good raising immunity, increasing leukocyte quantity, adjunct antineoplastic to live
Property.At present, glycoprotein how is extracted in the more efficiently blanching solution from sea cucumber, more inexpensive foundation is suitble to industrialization production
Novel technical method is an important research direction.
Invention content
It is an object of the invention to provide a kind of methods using Holothurian machining blanching solution extraction glycoprotein.
The technical measures taken of the present invention are:A kind of method using Holothurian machining blanching solution extraction glycoprotein, it is special
Sign is that it includes the following steps:（1）It collects sea cucumber blanching solution and is cooled to 4-10 DEG C rapidly, using centrifugation, removal sea
Insoluble matter in ginseng blanching solution obtains centrifuged supernatant；
（2）Centrifuged supernatant uses electrically-actuated device electrodialysis, and desalination makes conductance reach 300-1000 μ S/cm stoppings, obtaining
Desalination clear liquid；
（3）Desalination clear liquid uses vacuum concentration mode, is concentrated into solid content between 20-45%, obtains concentrate；
（4）Concentrate is freeze-dried or is spray-dried to obtain the first dry solid；
（5）First dry solid for 90-95% ethanol solutions or is surpassed using petroleum ether or n-hexane or mass percent
Critical CO2The means such as fluid extraction carry out ungrease treatment, obtain the second dry solid；
（6）Second dry solid is added in pure water, heating extraction obtains extract solution；
（7）After extraction, extract solution centrifuges successively through centrifuging Horizontal helical type centrifuge, disc centrifuge, tubular type
Machine removal precipitation, supernatant are surpassed by the membrane filtration of 10KDa, filtered solution by the filter membrane that molecular cut off is 5000Da
Trapped fluid is collected in filter.
（8）Sea cucumber glycoprotein is obtained after trapped fluid drying, between 80-90%, sugar contains protein content in the component after measured
Amount is between 10-20%.
The specific feature of the present invention also has, and Holothurian machining blanching solution refers to using produced during fresh sea cucumber processing blanching
Processing blanching solution as extraction material.The concrete operation step that Holothurian machining blanching solution obtains is cut away intestines for fresh sea cucumber through solution
Afterwards, it is put into boiling water and boils 10-30min, pull gained liquid after sea cucumber out.
Present invention has the advantages that by cooling down rapidly, using electrically-actuated device removing salinity, concentration after, freeze-drying or
Spray drying obtains dry matter, sea cucumber blanching solution fast eliminating salinity in the case of not putrid and deteriorated has been effectively ensured, later
Utilize petroleum ether or n-hexane or 90-95% ethanol solutions or supercritical CO2The means such as fluid extraction are effectively stripped of sea cucumber blanching
The ingredients such as the lipid in liquid；Membrane technology separation is recycled to obtain glycoprotein component of the molecular weight between 5000-10000Da,
The step has effectively removed the ingredients such as albumen, saponin(e, taurine, small peptide in sea cucumber blanching solution；Each processing step of the new method
Combination, greatly improve using sea cucumber blanching solution extraction glycoprotein efficiency, greatly reduce ethanol solution usage amount,
By this method extraction obtain sea cucumber suger protein content in protein content between 80-90%, sugared content 10-20% it
Between, caused quantity of leucocyte, which reduces, after pharmacological evaluation is measured to Chemotherapy of Tumor Patients has significant raising effect, dialogue
The normal patient of cell quantity has no effect.The technical method more reduces the extraction glycoprotein from Holothurian machining blanching solution
Production cost and equipment investment, product have high health care and medical value.
Specific embodiment
Embodiment 1：
A kind of method using Holothurian machining blanching solution extraction glycoprotein, it includes the following steps:（1）Collect Holothurian machining
Blanching solution 5000L is cooled to rapidly 4 DEG C, later successively through decanter centrifuge 3000rpm, disc centrifuge
6000rpm, tube centrifuge 12000rpm, centrifugation collect supernatant after removing the insoluble matter in sea cucumber blanching solution.
（2）Supernatant uses electrically-actuated device（The HC-EDR-3 types of Taicang HuaChen Water Treatment Facilities Co., Ltd.'s production）It carries out
Electrodialysis desalination stops to obtain desalination clear liquid when solution conductivity reaches 600 μ S/cm.
（3）For desalination clear liquid using being concentrated in vacuo, it is 30% to be concentrated into solid content, obtains concentrate；
（4）The first solid powder is obtained after concentrate is spray-dried.
（5）Above-mentioned first solid powder is added in 95% ethanol solutions of 600L, and for 24 hours, removing fat utilizes suction filtration for stirring
Equipment filters, and collects filter residue, the second solid powder is obtained after filter residue and drying.
（6）Second solid powder is added in 2000L pure water, is heated to 100 DEG C and 60min is kept to obtain extract solution,
Extract solution is rapidly cooled to 4 DEG C, first through Horizontal helical type centrifuge（3000rpm）, then through disk plate centrifuge（Rotating speed
6000rpm）, most afterwards through tube centrifuge（Rotating speed 12000rpm）, supernatant is collected after centrifugation.
（7）Supernatant obtains filtered solution by the membrane filtration of molecular cut off 10KDa, and filtered solution is by molecular cut off
The filter membrane of 5000Da carries out ultrafiltration, collects trapped fluid.
（8）Trapped fluid is sea cucumber glycoprotein after drying, after testing protein content 81%, polyoses content 12%.
Embodiment 2:
A kind of method using Holothurian machining blanching solution extraction glycoprotein, it includes the following steps:（1）Sea cucumber is collected to add
Work blanching solution 5000L, is cooled to rapidly 4 DEG C, later successively through decanter centrifuge 3000rpm, disc centrifuge
6000rpm, tube centrifuge 12000rpm, centrifugation are removed in sea cucumber blanching solution not after removing the insoluble matter in sea cucumber blanching solution
Supernatant is collected after molten object.
（2）Supernatant carries out electrodialysis desalination using electrically-actuated device, stops de- when solution conductivity reaches 500 μ S/cm
Salt clear liquid.
（3）For desalination clear liquid using being concentrated in vacuo, it is 35% to be concentrated into solid content, obtains concentrate.
（4）Concentrate obtains the first dry solid after vacuum freeze drying.
（5）First dry solid utilizes supercritical CO2Fluid extraction（Nantong Huaan HA420-40-200 types）, extraction temperature
At 50 DEG C, extraction pressure tank removes fat, must remove the second dry solid of fat degree in 30Mpa, extraction 1h.
（6）Second dry solid of above-mentioned removing fat is added in 2000L pure water, is heated to 100 DEG C of holdings
60min obtains extract solution, 4 DEG C after the rapid cooling of extract solution, first through Horizontal helical type centrifuge（3000rpm）, then through disk
Centrifuge（Rotating speed 6000rpm）, most afterwards through tube centrifuge（Rotating speed 12000rpm）, supernatant is collected after centrifugation.
（7）For supernatant by the membrane filtration of molecular cut off 10KDa, filtered solution passes through the filter that molecular cut off is 5000Da
Film carries out ultrafiltration, collects trapped fluid.
（8）Trapped fluid is sea cucumber glycoprotein after drying, after testing protein content 83%, polyoses content 13%.
Claims (2)
1. a kind of method using Holothurian machining blanching solution extraction glycoprotein, it is characterized in that it includes the following steps:（1）Collect sea
Ginseng blanching solution is cooled to rapidly 4-10 DEG C, and using centrifugation, the insoluble matter removed in sea cucumber blanching solution must centrifuge supernatant
Liquid；
（2）Centrifuged supernatant uses electrically-actuated device electrodialysis, and desalination makes conductance reach 300-1000 μ S/cm stoppings, obtaining desalination
Clear liquid；
（3）Desalination clear liquid uses vacuum concentration mode, is concentrated into solid content between 20-45%, obtains concentrate；
（4）Concentrate is freeze-dried or is spray-dried to obtain the first dry solid；
（5）Using petroleum ether or n-hexane or mass percent it is 90-95% ethanol solutions or overcritical by the first dry solid
CO2Fluid extraction means carry out ungrease treatment, obtain the second dry solid；
（6）Second dry solid is added in pure water, heating extraction obtains extract solution；
（7）After extraction, extract solution removes precipitation through centrifuge mode, supernatant by the membrane filtration of 10KDa,
Filtered solution carries out ultrafiltration by the filter membrane that molecular cut off is 5000Da, collects trapped fluid；
（8）Sea cucumber glycoprotein is obtained after trapped fluid drying.
2. the method according to claim 1 using Holothurian machining blanching solution extraction glycoprotein, it is characterized in that step（7）In
Centrifuge mode be by extract solution successively through centrifuge Horizontal helical type centrifuge, disc centrifuge, tube centrifuge
Removal precipitation.
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| CN201510061478.XA CN104610423B (en) | 2015-02-06 | 2015-02-06 | Utilize the method for Holothurian machining blanching solution extraction glycoprotein |
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| CN201510061478.XA CN104610423B (en) | 2015-02-06 | 2015-02-06 | Utilize the method for Holothurian machining blanching solution extraction glycoprotein |
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| CN104610423A CN104610423A (en) | 2015-05-13 |
| CN104610423B true CN104610423B (en) | 2018-06-19 |
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Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105029513A (en) * | 2015-08-20 | 2015-11-11 | 大连润伟海参研发科技有限公司 | Method for extracting saponin, polysaccharide, peptide and melanin in wastewater obtained by boiling sea cucumbers |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102406048A (en) * | 2011-11-29 | 2012-04-11 | 山东好当家海洋发展股份有限公司 | Method for preparing sea cucumber glycoprotein by using sea cucumber blanching solution |
| CN102657320A (en) * | 2012-04-16 | 2012-09-12 | 浙江工业大学 | Cryo-concentration method of juice obtained by processing organisms |
| CN103725740A (en) * | 2013-12-06 | 2014-04-16 | 山东好当家海洋发展股份有限公司 | Method for extracting glycoprotein by using scollop blanching liquid |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102406048A (en) * | 2011-11-29 | 2012-04-11 | 山东好当家海洋发展股份有限公司 | Method for preparing sea cucumber glycoprotein by using sea cucumber blanching solution |
| CN102657320A (en) * | 2012-04-16 | 2012-09-12 | 浙江工业大学 | Cryo-concentration method of juice obtained by processing organisms |
| CN103725740A (en) * | 2013-12-06 | 2014-04-16 | 山东好当家海洋发展股份有限公司 | Method for extracting glycoprotein by using scollop blanching liquid |
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